Cp4.1LG07g09030 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG07g09030
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	J domain-containing protein
Location	Cp4.1LG07: 8111681 .. 8114110 (+)
RNA-Seq Expression	Cp4.1LG07g09030
Synteny	Cp4.1LG07g09030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGAACGAAATCAACTTCCCGCGAACGCCGACAATGGAGGCGCTGTATCTCTCCACTTCAGCACCAACCGTTCTTCCATTTCGGAAAACCGTTGGCTCTCGCACCATTTCAAAATCCTCGATTCTCTTCCCTTCTTCATCAAAGTCTCGATTCGCAACGATTTGCAGGCAAAGAATTGATGGAGAAGATACGCCTCTCTCCAATGCTATGGATGCTTACAGATTGTTCGGCGTCGACCCGACCTGCTCCGAATCCGAGCTCAAAGCCGCTTTTCGAGCTAAAGTACGTAGGTTTCTCTCGTTGCGATGAAAAATCGAATTGTAGAATCTAGATTTGAGTTTTCGGAATATGATTGGGAAGCGATTAAGTGATTGGCTACTTAGGTTGATTGTAGAATTTTTGGTTTTCTGTACCGATTTTCAATTCGACTTCGGATTTCTGGCCGTGCAGGTGAAGCAATTCCATCCAGACGTGAATAGAGATGGAAATTATTCCGATTCTATGATTCGTCGTGTAATTCAGGCGTACGAGGTAGCTATTCATCTATAGTATCAAGAAGAAAATTGCGTGGTGCTTTTATCTCATTGGTAAATGGTGTTTCTTTGATAGGATGAACATTATGGCAATCATCATTCATGATGACCTTAATTTGATTGTTATCTCCGAGTCCTTAATTACATTGTAGAATTCAAAACTATTGTTCTGTTGTTCCGAGAGAAAGTGCATAGAGAGTAATCTACGAATGGAAAAGATCCCCATTAAAGATTAACGATGAAATATTCAAGCCATAAGTACTTGTTTCGATGCTGACACCACAAGAGACTGTAACAAGCTGGAACTATTTGTGTTCTGATGAAGAAATTGTTCACTTCTATCATCTATGTTATAGTCAATTATGAAACTCAACAATCACTATGCTTTCTACAATATGCAGATGTTATCCAGTTGCAGCCGAACCGAGTTCATTGAGAGGTATCGTTGTGCTTTTGGTGTTGTTCTCAATGTGTATAGAGCTGGTTAAAACTCTTGCAGCATTCATCATAGAATAAATTGTGTAATTACCATATTCTTGGAAAGTTTAGGGGAATTCTTAATTATAAATAAAAGGTTATAGATAATATATATAACTGAACGAAGAGTTCTCATTCTTTATAAAAGTGTGGAACCTCTCCCTAGCTACGTTTTCGTTGAGGGGAAGCGGGAAAGGCCAAAGAGGACAATATCTTCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATTAGAGCCAGATACCGGACGATGTGCTAGCGAAGAGGCTGAGCCTTGAAGGGGGTGGACACAGGCGAGCGGTGTGTCAACAAGCACGCTGGGCCCCGAAGGGGGGGTGGTGGATTTAAGGGATGATGGTGTGTCAGCAGGGGGGGATGATGGTGTGTATGGGGAGGAGAACGAAACATTCTTTATAAAGGTGTGGAAACCTCTTCGACGGGGGGTGATTGTGAGGTCCAACATCGGCTGGGGAGGAGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTTCGATGTGTTTTAAAAACTTTGAGGGAAGCTCAAGCGAAAGTCCAAAAAGGACAATATCTACTACGTGCGGGCTATTACAATAACAATGAATTACTTTTATTGACATTTTTAAATTCGATGGAGTTTTATGATTTCAAGTTTCAGTTCCTCATTCATTTTCATCATTCTATTTGGTGATATCTATTGTTCATTTTTTATGGAACTCTCACAATTAAACATATGAACTCAGGGAATGTTTAGATCCTTTTGAAAACCCAGAATGCGATGCATTTGATGTCTTTGTTAATGAGTTTCTTTGTGTTGGAAGAGGTACCATCTTTGCTCCATCTTATGAATGTTACAATTTAAGTTCTTTGTTACAATACAACAATCTACATGTGTTGTGTCATAACAATATGCAGGCTGCCCATATTCGTGTGTAGACAGAGCTCCCCATGTCTTCACTTTTGCTTCGTCCACCGGGACAGCCCGGGCGACTTCTCAAGGTTCGTTCGAGTGATAATATGCAATTATAATTTCAGTATTTTGAGGACATATGAAATGTGTATTATTCCAACTACGATTTCTTGATTTAGTTTCAATAAACTAGGACACGGTGAAGATTATCAGGTTCAAATTGCAGTTGGCCAATGCCCCAGAAGCTGTATACATTATGTAACACCTTTGCAAAGAATCATCCTGGAAGAGCTACTTGACAGGTACAAAAATATTCCAACTTAGATTCATAGGTACAAAAATATTCCAACTTAGATTCATGTTCGAAATTGTGATGTCCTACGTTGGTTGGGGAGGAGAATAAACCACCATTTATAAGCGTGTGGAAATCTTCCCCTAGAAGACGCGTTTTAAAGCCTTGAGGGGAAACCCGAAGGGGAAAGTCCAAAGAGGA

mRNA sequence

TGGAACGAAATCAACTTCCCGCGAACGCCGACAATGGAGGCGCTGTATCTCTCCACTTCAGCACCAACCGTTCTTCCATTTCGGAAAACCGTTGGCTCTCGCACCATTTCAAAATCCTCGATTCTCTTCCCTTCTTCATCAAAGTCTCGATTCGCAACGATTTGCAGGCAAAGAATTGATGGAGAAGATACGCCTCTCTCCAATGCTATGGATGCTTACAGATTGTTCGGCGTCGACCCGACCTGCTCCGAATCCGAGCTCAAAGCCGCTTTTCGAGCTAAAGTGAAGCAATTCCATCCAGACGTGAATAGAGATGGAAATTATTCCGATTCTATGATTCGTCGTGTAATTCAGGCGTACGAGATGTTATCCAGTTGCAGCCGAACCGAGTTCATTGAGAGGGAATGTTTAGATCCTTTTGAAAACCCAGAATGCGATGCATTTGATGTCTTTGTTAATGAGTTTCTTTGTGTTGGAAGAGGCTGCCCATATTCGTGTGTAGACAGAGCTCCCCATGTCTTCACTTTTGCTTCGTCCACCGGGACAGCCCGGGCGACTTCTCAAGGACACGGTGAAGATTATCAGGTTCAAATTGCAGTTGGCCAATGCCCCAGAAGCTGTATACATTATGTAACACCTTTGCAAAGAATCATCCTGGAAGAGCTACTTGACAGGTACAAAAATATTCCAACTTAGATTCATAGGTACAAAAATATTCCAACTTAGATTCATGTTCGAAATTGTGATGTCCTACGTTGGTTGGGGAGGAGAATAAACCACCATTTATAAGCGTGTGGAAATCTTCCCCTAGAAGACGCGTTTTAAAGCCTTGAGGGGAAACCCGAAGGGGAAAGTCCAAAGAGGA

Coding sequence (CDS)

ATGGAGGCGCTGTATCTCTCCACTTCAGCACCAACCGTTCTTCCATTTCGGAAAACCGTTGGCTCTCGCACCATTTCAAAATCCTCGATTCTCTTCCCTTCTTCATCAAAGTCTCGATTCGCAACGATTTGCAGGCAAAGAATTGATGGAGAAGATACGCCTCTCTCCAATGCTATGGATGCTTACAGATTGTTCGGCGTCGACCCGACCTGCTCCGAATCCGAGCTCAAAGCCGCTTTTCGAGCTAAAGTGAAGCAATTCCATCCAGACGTGAATAGAGATGGAAATTATTCCGATTCTATGATTCGTCGTGTAATTCAGGCGTACGAGATGTTATCCAGTTGCAGCCGAACCGAGTTCATTGAGAGGGAATGTTTAGATCCTTTTGAAAACCCAGAATGCGATGCATTTGATGTCTTTGTTAATGAGTTTCTTTGTGTTGGAAGAGGCTGCCCATATTCGTGTGTAGACAGAGCTCCCCATGTCTTCACTTTTGCTTCGTCCACCGGGACAGCCCGGGCGACTTCTCAAGGACACGGTGAAGATTATCAGGTTCAAATTGCAGTTGGCCAATGCCCCAGAAGCTGTATACATTATGTAACACCTTTGCAAAGAATCATCCTGGAAGAGCTACTTGACAGGTACAAAAATATTCCAACTTAG

Protein sequence

MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFIERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHGEDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIPT

Homology

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BLAST of Cp4.1LG07g09030 vs. ExPASy Swiss-Prot
Match: Q9FMX6 (Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC76 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 53/210 (25.24%), Postives = 95/210 (45.24%), Query Frame = 0


Query: 24  TISKSSILFPSSSKSRFATI-----CRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKA 83
           T+  S+  +P S+  +  T+      + R     + +++  D Y L G+D +  +S++K+
Sbjct: 10  TLPPSTATWPCSTSQKLITVRSPLKFKCRATSSSSSITD-FDLYDLLGIDRSSDKSQIKS 69

Query: 84  AFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFIERECLDPFE-------- 143
           A+RA  K+ HPD+  D  +  ++I  + +AY++LS     +  ++E     E        
Sbjct: 70  AYRALQKRCHPDIAGDPGHDMAII--LNEAYQLLSDPISRQAYDKEQAKLEELRGYTGKP 129

Query: 144 ------NPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHGEDYQ 203
                  PE +    FV+E  CV  GC   C   A   F   ++ G AR  +Q    + +
Sbjct: 130 IYSVWCGPETEQRAAFVDEVKCV--GC-LKCALCAEKTFAIETAYGRARVVAQWADPESK 189

Query: 204 VQIAVGQCPRSCIHYVTPLQRIILEELLDR 215
           ++ A+  CP  CI  V       LE L+ +
Sbjct: 190 IKEAIEACPVDCISMVERSDLAPLEFLMSK 213

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BLAST of Cp4.1LG07g09030 vs. ExPASy Swiss-Prot
Match: Q8EUM4 (Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-06
Identity = 32/101 (31.68%), Postives = 54/101 (53.47%), Query Frame = 0


Query: 55  LSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSS 114
           +S+  D Y + GV    ++ ++K+AFR K  Q+HPD N++ + ++   + V QAYE+LS 
Sbjct: 1   MSSKRDYYEVLGVSKDATDDQIKSAFRKKAMQYHPDRNKEPD-AEEKFKEVNQAYEVLSD 60

Query: 115 CSRTEFIERECLDPFE-----NPECDAFDVFVNEFLCVGRG 151
             +    +R   +  +         D FD+F N+F   GRG
Sbjct: 61  PDKRANYDRFGHEGVDGQFGGGAGFDPFDIF-NQFFGGGRG 99

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BLAST of Cp4.1LG07g09030 vs. ExPASy Swiss-Prot
Match: B8I304 (Chaperone protein DnaJ OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.5e-06
Identity = 23/63 (36.51%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 55  LSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSS 114
           +++  D Y + GVD   S++ELK A+R   K++HPDVN     +++  + V +AYE+LS 
Sbjct: 1   MADKRDYYEVLGVDKNASDAELKKAYRNLAKKYHPDVNPGDTTAEAKFKEVNEAYEILSD 60

Query: 115 CSR 118
             +
Sbjct: 61  SQK 63

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BLAST of Cp4.1LG07g09030 vs. ExPASy Swiss-Prot
Match: A1B4F0 (Chaperone protein DnaJ OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-06
Identity = 28/72 (38.89%), Postives = 38/72 (52.78%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTE 119
           D Y + GV    S  E+K A+RAK KQ HPD N+D   S++  + V +AYE L    +  
Sbjct: 5   DYYEVLGVARGASADEIKKAYRAKAKQLHPDRNKDCKVSEAAFKEVNEAYECLKDDQKKA 64

Query: 120 FIERECLDPFEN 132
             +R     FEN
Sbjct: 65  AYDRFGHAAFEN 76

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BLAST of Cp4.1LG07g09030 vs. ExPASy Swiss-Prot
Match: A3DF24 (Chaperone protein DnaJ OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 5.9e-06
Identity = 21/59 (35.59%), Postives = 37/59 (62.71%), Query Frame = 0


Query: 55  LSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 114
           ++   D Y + GVD   S++E+K A+R   KQ+HPD+N     +++  + + +AYE+LS
Sbjct: 1   MAGKRDYYEILGVDRGASDAEIKKAYRKLAKQYHPDMNPGDKAAEAKFKEINEAYEVLS 59

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BLAST of Cp4.1LG07g09030 vs. NCBI nr
Match: XP_023537184.1 (uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo] >XP_023537185.1 uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo] >XP_023537187.1 uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436 bits (1122), Expect = 1.18e-153
Identity = 214/219 (97.72%), Postives = 216/219 (98.63%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180
           IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180

Query: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 219

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BLAST of Cp4.1LG07g09030 vs. NCBI nr
Match: KAG6586261.1 (Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434 bits (1115), Expect = 1.38e-152
Identity = 212/219 (96.80%), Postives = 215/219 (98.17%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180
           IERECLDPFENPECDAFDVFVNEFLCVG+GCPYSCVDRAPH FTFASSTGTARATSQGHG
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVDRAPHAFTFASSTGTARATSQGHG 180

Query: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 219

[Close]

BLAST of Cp4.1LG07g09030 vs. NCBI nr
Match: XP_022937591.1 (uncharacterized protein LOC111443953 [Cucurbita moschata] >XP_022937592.1 uncharacterized protein LOC111443953 [Cucurbita moschata] >XP_022937593.1 uncharacterized protein LOC111443953 [Cucurbita moschata])

HSP 1 Score: 431 bits (1108), Expect = 1.61e-151
Identity = 211/219 (96.35%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALYLSTSAPTVLPFRKTVGSRTISKSSI FPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSIPFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180
           IERECLDPFENPECDAFDVFVNEFLCVG+GCPYSCVDRAPH FTFASSTGTARATSQGHG
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVDRAPHAFTFASSTGTARATSQGHG 180

Query: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 219

[Close]

BLAST of Cp4.1LG07g09030 vs. NCBI nr
Match: XP_022965891.1 (uncharacterized protein LOC111465640 [Cucurbita maxima])

HSP 1 Score: 426 bits (1096), Expect = 1.08e-149
Identity = 208/219 (94.98%), Postives = 213/219 (97.26%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALY STSAP VLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYFSTSAPPVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AY+LFGVDPTCSESELKAAFRAKVK+FHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYKLFGVDPTCSESELKAAFRAKVKEFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180
           IERECLDPFENPECDAFDVFVNEFLCVG+GCPYSCV RAPHVFTFASSTGTARATSQGHG
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVGRAPHVFTFASSTGTARATSQGHG 180

Query: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 219

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BLAST of Cp4.1LG07g09030 vs. NCBI nr
Match: KAA0047985.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 366 bits (940), Expect = 7.24e-126
Identity = 187/226 (82.74%), Postives = 197/226 (87.17%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGS-------RTISKSSILFPSSSKSRFATICRQRIDGEDT 60
           MEAL  ST   T LPFRKTVGS        +ISKSSILF SSS SRF  ICRQRI+GEDT
Sbjct: 1   MEALCFST---TFLPFRKTVGSFNDTSNFPSISKSSILFSSSSTSRFTAICRQRIEGEDT 60

Query: 61  PLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 120
           PLS  M AYRL G+DPTCSESELKAAFRAKVKQFHPDV R+GN SDSMIRRVIQAYEMLS
Sbjct: 61  PLSGPMAAYRLLGLDPTCSESELKAAFRAKVKQFHPDVTRNGNDSDSMIRRVIQAYEMLS 120

Query: 121 SCSRTEFIERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTAR 180
           S SRTEFIERECLDPFENPEC+AFDVFVNEFLCVG+GCPYSCVDRAPHVFTFASSTGTAR
Sbjct: 121 SYSRTEFIERECLDPFENPECEAFDVFVNEFLCVGKGCPYSCVDRAPHVFTFASSTGTAR 180

Query: 181 ATSQGHGEDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           ATSQGH EDYQ+Q+AVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 ATSQGHSEDYQLQMAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 223

[Close]

BLAST of Cp4.1LG07g09030 vs. ExPASy TrEMBL
Match: A0A6J1FBN2 (uncharacterized protein LOC111443953 OS=Cucurbita moschata OX=3662 GN=LOC111443953 PE=4 SV=1)

HSP 1 Score: 431 bits (1108), Expect = 7.78e-152
Identity = 211/219 (96.35%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALYLSTSAPTVLPFRKTVGSRTISKSSI FPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSIPFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180
           IERECLDPFENPECDAFDVFVNEFLCVG+GCPYSCVDRAPH FTFASSTGTARATSQGHG
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVDRAPHAFTFASSTGTARATSQGHG 180

Query: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 219

[Close]

BLAST of Cp4.1LG07g09030 vs. ExPASy TrEMBL
Match: A0A6J1HMW8 (uncharacterized protein LOC111465640 OS=Cucurbita maxima OX=3661 GN=LOC111465640 PE=4 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 5.25e-150
Identity = 208/219 (94.98%), Postives = 213/219 (97.26%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALY STSAP VLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYFSTSAPPVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AY+LFGVDPTCSESELKAAFRAKVK+FHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYKLFGVDPTCSESELKAAFRAKVKEFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180
           IERECLDPFENPECDAFDVFVNEFLCVG+GCPYSCV RAPHVFTFASSTGTARATSQGHG
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVGRAPHVFTFASSTGTARATSQGHG 180

Query: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 EDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 219

[Close]

BLAST of Cp4.1LG07g09030 vs. ExPASy TrEMBL
Match: A0A5A7U2T5 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00480 PE=4 SV=1)

HSP 1 Score: 366 bits (940), Expect = 3.51e-126
Identity = 187/226 (82.74%), Postives = 197/226 (87.17%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGS-------RTISKSSILFPSSSKSRFATICRQRIDGEDT 60
           MEAL  ST   T LPFRKTVGS        +ISKSSILF SSS SRF  ICRQRI+GEDT
Sbjct: 1   MEALCFST---TFLPFRKTVGSFNDTSNFPSISKSSILFSSSSTSRFTAICRQRIEGEDT 60

Query: 61  PLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 120
           PLS  M AYRL G+DPTCSESELKAAFRAKVKQFHPDV R+GN SDSMIRRVIQAYEMLS
Sbjct: 61  PLSGPMAAYRLLGLDPTCSESELKAAFRAKVKQFHPDVTRNGNDSDSMIRRVIQAYEMLS 120

Query: 121 SCSRTEFIERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTAR 180
           S SRTEFIERECLDPFENPEC+AFDVFVNEFLCVG+GCPYSCVDRAPHVFTFASSTGTAR
Sbjct: 121 SYSRTEFIERECLDPFENPECEAFDVFVNEFLCVGKGCPYSCVDRAPHVFTFASSTGTAR 180

Query: 181 ATSQGHGEDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           ATSQGH EDYQ+Q+AVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 ATSQGHSEDYQLQMAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 223

[Close]

BLAST of Cp4.1LG07g09030 vs. ExPASy TrEMBL
Match: A0A0A0LJM6 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G022810 PE=4 SV=1)

HSP 1 Score: 365 bits (937), Expect = 1.00e-125
Identity = 187/226 (82.74%), Postives = 197/226 (87.17%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGS-------RTISKSSILFPSSSKSRFATICRQRIDGEDT 60
           MEAL  ST   TVLPFRKTVGS        +ISKSSILFPSSS SRF  ICRQR +GEDT
Sbjct: 1   MEALCFST---TVLPFRKTVGSFNDTSNFPSISKSSILFPSSSTSRFTPICRQRTEGEDT 60

Query: 61  PLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 120
            LS  M AYRL G+DPTCSESELKAAFRAKVKQFHPDVNR+GN SDSMIRRVIQAYEMLS
Sbjct: 61  LLSGPMAAYRLLGLDPTCSESELKAAFRAKVKQFHPDVNRNGNDSDSMIRRVIQAYEMLS 120

Query: 121 SCSRTEFIERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTAR 180
           S SRTEFIERECLDPFENPEC+AFDVFVNEFLCVG+GCPYSCVDRAPHVFTFASSTGTAR
Sbjct: 121 SYSRTEFIERECLDPFENPECEAFDVFVNEFLCVGKGCPYSCVDRAPHVFTFASSTGTAR 180

Query: 181 ATSQGHGEDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           A SQGH EDYQ+Q+AVGQCPRSCIHYVTPLQRIILEELLD   ++P
Sbjct: 181 AMSQGHSEDYQLQMAVGQCPRSCIHYVTPLQRIILEELLDSALDVP 223

[Close]

BLAST of Cp4.1LG07g09030 vs. ExPASy TrEMBL
Match: A0A6J1D9D8 (pentatricopeptide repeat-containing protein At3g05340 OS=Momordica charantia OX=3673 GN=LOC111018815 PE=3 SV=1)

HSP 1 Score: 363 bits (931), Expect = 2.31e-116
Identity = 185/228 (81.14%), Postives = 194/228 (85.09%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSR-------TISKSSILFPSSSKS--RFATICRQRIDGE 60
           M AL    S PT+LPFRKTVGS        + SK SILFPSSS S  RFATICRQR +GE
Sbjct: 684 MAALCFYASTPTILPFRKTVGSPNDSSNFPSKSKPSILFPSSSSSASRFATICRQRSEGE 743

Query: 61  DTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEM 120
           D PLS A+ AYR+ GVDPTCS+SELKAAFRAKVKQFHPDVN +GN SDSMIRRVIQAYEM
Sbjct: 744 DAPLSTALAAYRVLGVDPTCSQSELKAAFRAKVKQFHPDVNGNGNASDSMIRRVIQAYEM 803

Query: 121 LSSCSRTEFIERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGT 180
           LSS SRTEFIERECLDPFENPEC AFDVFVNEFLCVG+GCPYSCVD APHVFTFASSTGT
Sbjct: 804 LSSYSRTEFIERECLDPFENPECQAFDVFVNEFLCVGKGCPYSCVDTAPHVFTFASSTGT 863

Query: 181 ARATSQGHGEDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDRYKNIP 219
           ARATSQGHGEDYQV IA GQCPRSCIHYVTPLQRIILEELLD   N+P
Sbjct: 864 ARATSQGHGEDYQVLIAAGQCPRSCIHYVTPLQRIILEELLDSALNVP 911

[Close]

BLAST of Cp4.1LG07g09030 vs. TAIR 10
Match: AT3G05345.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 2.9e-64
Identity = 119/185 (64.32%), Postives = 144/185 (77.84%), Query Frame = 0


Query: 35  SSKSRFATICRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRD 94
           S+  RF   CR +    + PLS++   Y + GV+P+CS SELKAAFRAKVKQ+HPDVN+D
Sbjct: 22  STSQRFIPSCRGK--NREDPLSSS-SPYSILGVEPSCSSSELKAAFRAKVKQYHPDVNKD 81

Query: 95  GNYSDSMIRRVIQAYEMLSSCSRTEFIERECLDPFENPECDAFDVFVNEFLCVGRGCPYS 154
           G+ SD MIRR+IQAYEML++ SR+E IE ECLDPF++PEC+A DVFVNE LCVG+ C Y 
Sbjct: 82  GSNSDIMIRRIIQAYEMLTNYSRSEIIEGECLDPFDHPECEALDVFVNEVLCVGKRCSYP 141

Query: 155 CVDRAPHVFTFASSTGTARATSQGHGEDYQVQIAVGQCPRSCIHYVTPLQRIILEELLDR 214
           C + A HVF+   S+GTARA SQGHGEDY+VQ AV QCPR+CIHYVTP QRIILEELLD 
Sbjct: 142 CFETASHVFS-CDSSGTARAMSQGHGEDYRVQSAVNQCPRNCIHYVTPSQRIILEELLDS 201

Query: 215 YKNIP 220
             + P
Sbjct: 202 VVDKP 202

[Close]

BLAST of Cp4.1LG07g09030 vs. TAIR 10
Match: AT2G42750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-14
Identity = 64/211 (30.33%), Postives = 100/211 (47.39%), Query Frame = 0


Query: 18  KTVGSRTISKSSILFPSSSKSRFATICRQRIDGED-TPLSN---AMDAYRLFGVDPTCSE 77
           KT G+   S S  L   + K R  +I R R+  ED + LS    A D Y + G+ P  ++
Sbjct: 33  KTTGA---SSSWYLPRYAGKRRTDSIGRLRVATEDASSLSTGDVADDYYAVLGLLPDATQ 92

Query: 78  SELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFIER------ECLD 137
            E+K A+   +K  HPD++ +   + +    +   YE+LS   +    +         ++
Sbjct: 93  EEIKKAYYNCMKSCHPDLSGNDPETTNFCMFINDIYEILSDPVQRMVYDEIHGYTVTAIN 152

Query: 138 PFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHGEDYQVQI 197
           PF +       VFV+EF C+  GC  +C + AP +F      G ARA +Q  G    VQ 
Sbjct: 153 PFLDDSTPRDHVFVDEFACI--GCK-NCANVAPDIFQIEEDFGRARACNQ-RGNPDLVQQ 212

Query: 198 AVGQCPRSCIHYVTPLQRIILEELLDRYKNI 219
           AV  CP  CIH  +  Q  +LE+ + R + +
Sbjct: 213 AVETCPVDCIHQTSAAQLSLLEDEMRRVERV 236

[Close]

BLAST of Cp4.1LG07g09030 vs. TAIR 10
Match: AT5G23240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 65.1 bits (157), Expect = 8.1e-11
Identity = 53/210 (25.24%), Postives = 95/210 (45.24%), Query Frame = 0


Query: 24  TISKSSILFPSSSKSRFATI-----CRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKA 83
           T+  S+  +P S+  +  T+      + R     + +++  D Y L G+D +  +S++K+
Sbjct: 10  TLPPSTATWPCSTSQKLITVRSPLKFKCRATSSSSSITD-FDLYDLLGIDRSSDKSQIKS 69

Query: 84  AFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFIERECLDPFE-------- 143
           A+RA  K+ HPD+  D  +  ++I  + +AY++LS     +  ++E     E        
Sbjct: 70  AYRALQKRCHPDIAGDPGHDMAII--LNEAYQLLSDPISRQAYDKEQAKLEELRGYTGKP 129

Query: 144 ------NPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHGEDYQ 203
                  PE +    FV+E  CV  GC   C   A   F   ++ G AR  +Q    + +
Sbjct: 130 IYSVWCGPETEQRAAFVDEVKCV--GC-LKCALCAEKTFAIETAYGRARVVAQWADPESK 189

Query: 204 VQIAVGQCPRSCIHYVTPLQRIILEELLDR 215
           ++ A+  CP  CI  V       LE L+ +
Sbjct: 190 IKEAIEACPVDCISMVERSDLAPLEFLMSK 213

[Close]

BLAST of Cp4.1LG07g09030 vs. TAIR 10
Match: AT1G28210.1 (DNAJ heat shock family protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.1e-06
Identity = 22/64 (34.38%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 56  SNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSC 115
           S+A + Y + GV P  +  E+K +F    K+FHPD NR+   +    + + +AYE L + 
Sbjct: 44  SSARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRNNPSAKRKFQEIREAYETLGNS 103

Query: 116 SRTE 120
            R E
Sbjct: 104 ERRE 107

[Close]

BLAST of Cp4.1LG07g09030 vs. TAIR 10
Match: AT1G28210.2 (DNAJ heat shock family protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.1e-06
Identity = 22/64 (34.38%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 56  SNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSC 115
           S+A + Y + GV P  +  E+K +F    K+FHPD NR+   +    + + +AYE L + 
Sbjct: 44  SSARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRNNPSAKRKFQEIREAYETLGNS 103

Query: 116 SRTE 120
            R E
Sbjct: 104 ERRE 107

The following BLAST results are available for this feature:

BLAST of Cp4.1LG07g09030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FMX6	1.1e-09	25.24	Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC...	[more]
Q8EUM4	1.5e-06	31.68	Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ P...	[more]
B8I304	1.5e-06	36.51	Chaperone protein DnaJ OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / ...	[more]
A1B4F0	2.6e-06	38.89	Chaperone protein DnaJ OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN...	[more]
A3DF24	5.9e-06	35.59	Chaperone protein DnaJ OS=Hungateiclostridium thermocellum (strain ATCC 27405 / ...	[more]

BLAST of Cp4.1LG07g09030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537184.1	1.18e-153	97.72	uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo] >XP_023537185....	[more]
KAG6586261.1	1.38e-152	96.80	Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp...	[more]
XP_022937591.1	1.61e-151	96.35	uncharacterized protein LOC111443953 [Cucurbita moschata] >XP_022937592.1 unchar...	[more]
XP_022965891.1	1.08e-149	94.98	uncharacterized protein LOC111465640 [Cucurbita maxima]	[more]
KAA0047985.1	7.24e-126	82.74	pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa]	[more]

BLAST of Cp4.1LG07g09030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FBN2	7.78e-152	96.35	uncharacterized protein LOC111443953 OS=Cucurbita moschata OX=3662 GN=LOC1114439...	[more]
A0A6J1HMW8	5.25e-150	94.98	uncharacterized protein LOC111465640 OS=Cucurbita maxima OX=3661 GN=LOC111465640...	[more]
A0A5A7U2T5	3.51e-126	82.74	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0LJM6	1.00e-125	82.74	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G022810 PE=4 SV=1	[more]
A0A6J1D9D8	2.31e-116	81.14	pentatricopeptide repeat-containing protein At3g05340 OS=Momordica charantia OX=...	[more]

BLAST of Cp4.1LG07g09030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05345.1	2.9e-64	64.32	Chaperone DnaJ-domain superfamily protein	[more]
AT2G42750.1	1.6e-14	30.33	DNAJ heat shock N-terminal domain-containing protein	[more]
AT5G23240.1	8.1e-11	25.24	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G28210.1	2.1e-06	34.38	DNAJ heat shock family protein	[more]
AT1G28210.2	2.1e-06	34.38	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 62..80 score: 34.98 coord: 80..95 score: 35.33 coord: 97..117 score: 22.9
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 59..117 e-value: 2.5E-12 score: 57.0
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 60..114 e-value: 2.1E-10 score: 40.5
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 60..130 score: 13.571817
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 60..113 e-value: 7.12506E-14 score: 61.7925
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 49..130 e-value: 4.2E-13 score: 51.2
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 56..127
None	No IPR available	GENE3D	3.30.70.20		coord: 138..200 e-value: 1.1E-11 score: 46.8
None	No IPR available	PANTHER	PTHR45295	CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTIC	coord: 20..215
None	No IPR available	PANTHER	PTHR45295:SF3	CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN	coord: 20..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG07g09030.1	Cp4.1LG07g09030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0008270	zinc ion binding