Cp4.1LG07g05450 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG07g05450 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 6.1e-46
Identity = 97/261 (37.16%), Postives = 152/261 (58.24%), Query Frame = 0

Query: 253 PTNIFILAGQSNMAGRGGVEK-IQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPL 312
           P  IFIL+GQSNMAGRGGV K   N + VWD  +P EC  + SILRL+ + +WE AHEPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 313 HLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFY 372
           H+ ID     G+GPG+ FA+ +K +    + ++GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 373 QNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPV 432
           +  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R+D+    LP+
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 433 IIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWDHGH 492
           I V I+    ++          +  E  +  +L +++ +D++ LP+          D+ H
Sbjct: 201 IQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS--------DNLH 259

Query: 493 FNTETEIVLGKWLADTYLAHY 513
             TE ++ LG  LA  YL+++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Cp4.1LG07g05450 vs. NCBI nr
Match: KAG6585684.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 835 bits (2156), Expect = 2.11e-301
Identity = 413/549 (75.23%), Postives = 449/549 (81.79%), Query Frame = 0

Query: 1   MAGRGGVEKNQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDISNTPGIG 60
           MAGRGGVE N  G LVWDGKVPL+CQSDPSILRLNPERQWEIAHEPLHLGIDI  TPGIG
Sbjct: 1   MAGRGGVENNTEGNLVWDGKVPLDCQSDPSILRLNPERQWEIAHEPLHLGIDIGKTPGIG 60

Query: 61  PGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 120
           PGI FAH+   KAG+KAG+VGLVPCARGGTLIEQW KNPSN SATFYQNFIERIKTSEKE
Sbjct: 61  PGIAFAHEFIAKAGKKAGVVGLVPCARGGTLIEQWSKNPSNTSATFYQNFIERIKTSEKE 120

Query: 121 GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIVVKIALYDFFMK 180
           GGVVRALFWYQGESDAAM+DTA RYKDNLKKFITDIRNDIKPRFLPVI+VKI++YDFFMK
Sbjct: 121 GGVVRALFWYQGESDAAMSDTAHRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMK 180

Query: 181 HDTHNLPAVRATEDAVQKELPDIITIDSWTLPINLTTFEGFSWDHGHFNTETEIVLGKW- 240
           HDTH+LPAVRA EDAVQKELPDIITIDSW LPIN TT+EGFSWDHGHFN+ TEI L    
Sbjct: 181 HDTHDLPAVRAAEDAVQKELPDIITIDSWELPINFTTYEGFSWDHGHFNSATEIALALEP 240

Query: 241 ------------------LADTYLAHYG--------------ATSPTNIFILAGQSNMAG 300
                              A   LA  G              ATSP NIFILAGQSNMAG
Sbjct: 241 VHDGIDINKTVRVVSAIVFARQLLAKSGSKAGVVGLVPCARGATSPKNIFILAGQSNMAG 300

Query: 301 RGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDISNTPGIGPGI 360
           RGG+EK   G+ VWDG +P E QSDP+ILR +PE QWEIA EP+H GIDI+ T G+G  I
Sbjct: 301 RGGIEKDHTGQRVWDGYIPPEAQSDPTILRFSPEGQWEIALEPVHDGIDINKTVGVGSAI 360

Query: 361 PFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGV 420
            FA QL+ K+  KAG+VGLVPCARGGTLIE+WIKNPSNP ATFYQNFIERIK SEK+GGV
Sbjct: 361 VFARQLQAKSESKAGVVGLVPCARGGTLIEEWIKNPSNPKATFYQNFIERIKASEKDGGV 420

Query: 421 VRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDT 480
           VRAL W QGESDAA  DTA+RYKDNLKKF  DIRNDIKPRFLP+I+VKI++YD +MKHDT
Sbjct: 421 VRALIWLQGESDAAARDTARRYKDNLKKFFMDIRNDIKPRFLPIILVKIAVYDTYMKHDT 480

Query: 481 HNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLADT 516
           H+LPAVRAAEDAVQKELPDI+TIDS  L VN  T EGF+ DH HFNT+T+I LGKWLADT
Sbjct: 481 HDLPAVRAAEDAVQKELPDIVTIDSINL-VNTITHEGFNQDHIHFNTKTQIALGKWLADT 540

BLAST of Cp4.1LG07g05450 vs. NCBI nr
Match: KAE8652071.1 (hypothetical protein Csa_018776 [Cucumis sativus])

HSP 1 Score: 761 bits (1964), Expect = 1.25e-267
Identity = 405/737 (54.95%), Postives = 445/737 (60.38%), Query Frame = 0

Query: 1   MAGRGGVEKNQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDISNTPGIG 60
           MAGRGGVE N  G L WDG VP ECQ  PSILRLNP  QWEIA EPLHLGIDI  TPGIG
Sbjct: 37  MAGRGGVENNAQGNLQWDGLVPPECQPQPSILRLNPGLQWEIAREPLHLGIDIKRTPGIG 96

Query: 61  PGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 120
           PGI FAH+L  KAG  AG VGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIK S+K+
Sbjct: 97  PGIAFAHELLVKAGPNAGAVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKASDKD 156

Query: 121 GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIVVKIALYDFFMK 180
           GGVVRALFW+QGESDAAMNDTA RYKDNLKKF TDIR+DIKPRFLP+IVVKIALYDFF +
Sbjct: 157 GGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRDDIKPRFLPIIVVKIALYDFFRQ 216

Query: 181 HDTHNLPAVRATEDAVQKELPDIITIDSWTLPINLTTFEGFSWDHGHFNTETEIVLGKWL 240
           HDTHNLPAVR  ++AV KELP+++ IDS  LPIN TT EG + DHGHFNT TEI LGKWL
Sbjct: 217 HDTHNLPAVREAQEAVSKELPNVVAIDSLKLPINYTTNEGINLDHGHFNTTTEITLGKWL 276

Query: 241 ADTYLAHYGATSPTNIFILAGQSNMAGRGGVEK--------------------------- 300
           A+TYL+H+GA SP NIFILAGQSNMAGRGGVE                            
Sbjct: 277 AETYLSHFGAASPKNIFILAGQSNMAGRGGVENNAQGNLQWDGLVPPECQPQPSILRLNP 336

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 337 GLQWEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKVGPNAGAVGLVPCARASDKDGGVV 396

Query: 361 ------------------------------------------------------------ 420
                                                                       
Sbjct: 397 RALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRDDIKPRFLPIIVVKIALYDFFRQHDTH 456

Query: 421 ------------------------------------------------------------ 480
                                                                       
Sbjct: 457 NLPAVREAQEAVSKELPDVVAIDSLKLPINYTTNEGINLDHGHFNTTTEITLGAASPKNI 516

Query: 481 --------------IQN---GKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGID 513
                         ++N   G L WDG VP ECQ  PSILRLNP  QWEIA EPLHLGID
Sbjct: 517 FILAGQSNMAGRGGVENNAQGNLQWDGLVPPECQPQPSILRLNPGLQWEIAREPLHLGID 576

BLAST of Cp4.1LG07g05450 vs. NCBI nr
Match: KAG6585825.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552 bits (1422), Expect = 4.22e-194
Identity = 265/268 (98.88%), Postives = 267/268 (99.63%), Query Frame = 0

Query: 249 GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 308
           GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH
Sbjct: 10  GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 69

Query: 309 EPLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 368
           EPLHLGIDIS+TPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA
Sbjct: 70  EPLHLGIDISHTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 129

Query: 369 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 428
           TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF
Sbjct: 130 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 189

Query: 429 LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 488
           LPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD
Sbjct: 190 LPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 249

Query: 489 HGHFNTETEIVLGKWLADTYLAHYGHLL 516
           HGHFNTETEIVLGKWLADTYL HYGHLL
Sbjct: 250 HGHFNTETEIVLGKWLADTYLTHYGHLL 277

BLAST of Cp4.1LG07g05450 vs. NCBI nr
Match: KAG6585849.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 549 bits (1414), Expect = 6.94e-193
Identity = 263/268 (98.13%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 249 GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 308
           GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH
Sbjct: 10  GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 69

Query: 309 EPLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 368
           EPLHLGIDIS+TPGIGPGIPFAHQLKEKAGQKAG VGLVPCARGGTLIEQW+KNPSNPSA
Sbjct: 70  EPLHLGIDISHTPGIGPGIPFAHQLKEKAGQKAGTVGLVPCARGGTLIEQWMKNPSNPSA 129

Query: 369 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 428
           TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF
Sbjct: 130 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 189

Query: 429 LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 488
           LPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD
Sbjct: 190 LPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 249

Query: 489 HGHFNTETEIVLGKWLADTYLAHYGHLL 516
           HGHFNTETEIVLGKWLADTYL HYGHLL
Sbjct: 250 HGHFNTETEIVLGKWLADTYLTHYGHLL 277

BLAST of Cp4.1LG07g05450 vs. NCBI nr
Match: XP_023536887.1 (probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023536888.1 probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023536889.1 probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023536890.1 probable carbohydrate esterase At4g34215 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023537924.1 probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548 bits (1413), Expect = 1.48e-192
Identity = 263/268 (98.13%), Postives = 265/268 (98.88%), Query Frame = 0

Query: 249 GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 308
           GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH
Sbjct: 21  GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 80

Query: 309 EPLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 368
           EPLHLGIDIS+TPGIGPGIPFAHQ KEKAGQKAG VGLVPCARGGTLIEQWIKNPSNPSA
Sbjct: 81  EPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTLIEQWIKNPSNPSA 140

Query: 369 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 428
           TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF
Sbjct: 141 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 200

Query: 429 LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 488
           LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELP+N TTFEGFSWD
Sbjct: 201 LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPINLTTFEGFSWD 260

Query: 489 HGHFNTETEIVLGKWLADTYLAHYGHLL 516
           HGHFNTETEIVLGKWLADTYLAHYGHLL
Sbjct: 261 HGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Cp4.1LG07g05450 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 548 bits (1411), Expect = 1.44e-192
Identity = 263/268 (98.13%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 249 GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 308
           GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQ DPSILRLNPERQWEIAH
Sbjct: 21  GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQFDPSILRLNPERQWEIAH 80

Query: 309 EPLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 368
           EPLHLGIDIS+TPGIGPGIPFAHQ KEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA
Sbjct: 81  EPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 140

Query: 369 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 428
           TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF
Sbjct: 141 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 200

Query: 429 LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 488
           LPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD
Sbjct: 201 LPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 260

Query: 489 HGHFNTETEIVLGKWLADTYLAHYGHLL 516
           HGHFNTETEIVLGKWLA+TYLAHYGHLL
Sbjct: 261 HGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Cp4.1LG07g05450 vs. ExPASy TrEMBL
Match: A0A6J1GIT2 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454649 PE=4 SV=1)

HSP 1 Score: 543 bits (1399), Expect = 9.61e-191
Identity = 261/268 (97.39%), Postives = 265/268 (98.88%), Query Frame = 0

Query: 249 GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 308
           GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLEC S+PSILRLNPERQWEIAH
Sbjct: 21  GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECLSNPSILRLNPERQWEIAH 80

Query: 309 EPLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSA 368
           EPLHLGIDIS+TPGIGPGIPFAHQ KEKAGQKAGIVGLVPCARGGTLIEQW+KNPSNPSA
Sbjct: 81  EPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTLIEQWMKNPSNPSA 140

Query: 369 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 428
           TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF
Sbjct: 141 TFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRF 200

Query: 429 LPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 488
           LPVIIVKIS+YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD
Sbjct: 201 LPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWD 260

Query: 489 HGHFNTETEIVLGKWLADTYLAHYGHLL 516
            GHFNTETEIVLGKWLADTYLAHYGHLL
Sbjct: 261 RGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of Cp4.1LG07g05450 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 8.24e-183
Identity = 252/270 (93.33%), Postives = 258/270 (95.56%), Query Frame = 0

Query: 249 GATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAH 308
           GATSPTNIFILAGQSNMAGRGGVEK   G+LVWDGKVP ECQSDPSILR NPERQWEIAH
Sbjct: 21  GATSPTNIFILAGQSNMAGRGGVEKTPTGELVWDGKVPSECQSDPSILRFNPERQWEIAH 80

Query: 309 EPLHLGIDI--SNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNP 368
           EPLHLGID+  + TPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNP
Sbjct: 81  EPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNP 140

Query: 369 SATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKP 428
           SATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKP
Sbjct: 141 SATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKP 200

Query: 429 RFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFS 488
           RFLPVIIVKI++YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDS ELP+N TTFEGFS
Sbjct: 201 RFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTTFEGFS 260

Query: 489 WDHGHFNTETEIVLGKWLADTYLAHYGHLL 516
           WDHGHFNT TEI LGKWLADTYLAHYGHLL
Sbjct: 261 WDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Cp4.1LG07g05450 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 509 bits (1312), Expect = 1.59e-177
Identity = 247/267 (92.51%), Postives = 250/267 (93.63%), Query Frame = 0

Query: 250 ATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHE 309
           ATSPTNIFILAGQSNMAGRGGVE  Q GKL WDGKVPLECQSDPSILRLNP RQWEIA E
Sbjct: 22  ATSPTNIFILAGQSNMAGRGGVENNQKGKLEWDGKVPLECQSDPSILRLNPARQWEIAQE 81

Query: 310 PLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSAT 369
           PLHLGIDI  TPGIGPGIPFAHQ K KAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSAT
Sbjct: 82  PLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSAT 141

Query: 370 FYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFL 429
           FYQNFIERIKTSEKEGGVVRALFWYQGESDAAM+DTA RYKDNLKKFITDIRNDIKPRFL
Sbjct: 142 FYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFITDIRNDIKPRFL 201

Query: 430 PVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWDH 489
           PVIIVKISMYDFFMKHDTH+LPAVRAAEDAVQKELPDIITIDS ELP+NFTTFEGF  DH
Sbjct: 202 PVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWELPINFTTFEGFCLDH 261

Query: 490 GHFNTETEIVLGKWLADTYLAHYGHLL 516
           GHFNT TEI LGKWLADTYLAHY HLL
Sbjct: 262 GHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Cp4.1LG07g05450 vs. ExPASy TrEMBL
Match: A0A6J1GK48 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454647 PE=4 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 2.66e-174
Identity = 238/252 (94.44%), Postives = 243/252 (96.43%), Query Frame = 0

Query: 265 MAGRGGVEKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDISNTPGIG 324
           MAGRGGVEK QNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDI +TPGIG
Sbjct: 1   MAGRGGVEKTQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPLHLGIDIGHTPGIG 60

Query: 325 PGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 384
            GIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE
Sbjct: 61  SGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKE 120

Query: 385 GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMK 444
           GGVVRALFWYQGESDAAMNDTAQRYK+NLKKFITDIRNDIKPRFLPVIIVKI++YDFFMK
Sbjct: 121 GGVVRALFWYQGESDAAMNDTAQRYKENLKKFITDIRNDIKPRFLPVIIVKIALYDFFMK 180

Query: 445 HDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWL 504
           HDTHNLPAVR AEDAVQKELPDIITIDS ELP+N TTFEGFSWDHGH NT TEI LGKWL
Sbjct: 181 HDTHNLPAVREAEDAVQKELPDIITIDSLELPINLTTFEGFSWDHGHLNTATEIALGKWL 240

Query: 505 ADTYLAHYGHLL 516
           ADTYLAHYGHLL
Sbjct: 241 ADTYLAHYGHLL 252

BLAST of Cp4.1LG07g05450 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 186.8 bits (473), Expect = 4.4e-47
Identity = 97/261 (37.16%), Postives = 152/261 (58.24%), Query Frame = 0

Query: 253 PTNIFILAGQSNMAGRGGVEK-IQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPL 312
           P  IFIL+GQSNMAGRGGV K   N + VWD  +P EC  + SILRL+ + +WE AHEPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 313 HLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFY 372
           H+ ID     G+GPG+ FA+ +K +    + ++GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 373 QNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPV 432
           +  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R+D+    LP+
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 433 IIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWDHGH 492
           I V I+    ++          +  E  +  +L +++ +D++ LP+          D+ H
Sbjct: 201 IQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS--------DNLH 259

Query: 493 FNTETEIVLGKWLADTYLAHY 513
             TE ++ LG  LA  YL+++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Cp4.1LG07g05450 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 186.8 bits (473), Expect = 4.4e-47
Identity = 97/261 (37.16%), Postives = 152/261 (58.24%), Query Frame = 0

Query: 253 PTNIFILAGQSNMAGRGGVEK-IQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHEPL 312
           P  IFIL+GQSNMAGRGGV K   N + VWD  +P EC  + SILRL+ + +WE AHEPL
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 313 HLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFY 372
           H+ ID     G+GPG+ FA+ +K +    + ++GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 373 QNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPV 432
           +  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R+D+    LP+
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 433 IIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSWDHGH 492
           I V I+    ++          +  E  +  +L +++ +D++ LP+          D+ H
Sbjct: 201 IQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS--------DNLH 259

Query: 493 FNTETEIVLGKWLADTYLAHY 513
             TE ++ LG  LA  YL+++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Cp4.1LG07g05450 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 186.4 bits (472), Expect = 5.7e-47
Identity = 108/263 (41.06%), Postives = 153/263 (58.17%), Query Frame = 0

Query: 251 TSPTNIFILAGQSNMAGRGGV-EKIQNGKLVWDGKVPLECQSDPSILRLNPERQWEIAHE 310
           T   +IFILAGQSNMAGRGGV         VWDG +P EC+S+PSILRL  + +W+ A E
Sbjct: 26  TRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKE 85

Query: 311 PLHLGIDISNTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSAT 370
           PLH+ IDI+ T G+GPG+PFA+++  + GQ    VGLVPC+ GGT + QW K        
Sbjct: 86  PLHVDIDINKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQK-----GEF 145

Query: 371 FYQNFIERIKTSEKE--GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPR 430
            Y+  ++R K +     GG  RA+ WYQGESD      A  YK  L KF +D+RND++  
Sbjct: 146 LYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHP 205

Query: 431 FLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEGFSW 490
            LP+I V ++            L AVR A+  ++ +L ++  +D+R LP+          
Sbjct: 206 NLPIIQVALA------TGAGPYLDAVRKAQ--LKTDLENVYCVDARGLPL--------EP 263

Query: 491 DHGHFNTETEIVLGKWLADTYLA 511
           D  H  T +++ LG  +A+++LA
Sbjct: 266 DGLHLTTSSQVQLGHMIAESFLA 263