Cp4.1LG07g05300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG07g05300 vs. ExPASy Swiss-Prot
Match: F4JLM5 (Isoleucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g10320 PE=2 SV=1)

HSP 1 Score: 1800.4 bits (4662), Expect = 0.0e+00
Identity = 917/1443 (63.55%), Postives = 1042/1443 (72.21%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL+EK LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMVTFPV+ D  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NA F+YVKVRNK +GKV++VA+SRLSALP +K K    NGP   +KK+NPK KG+     
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDTKKANPKAKGAK---- 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
              S  DS+E+LE+  GASLVGKKYEPLFDYF +FS  AFRVVAD YVTDDSGTGIVHCAP
Sbjct: 301  PESAADSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVC+ N++I K                                          
Sbjct: 361  AFGEDDYRVCLLNKIIKK------------------------------------------ 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                    GENL
Sbjct: 541  --------------------------------------------------------GENL 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
            +VAVDDDG FT +IT FSGRYVKDADKDIIEAVKA+GRLVK+GSFTHSYPFCWRSDTPLI
Sbjct: 601  VVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKGRLVKTGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVE+LKEKLL++NE+T WVP 
Sbjct: 661  YRAVPSW------------------------------FVRVEQLKEKLLKSNEQTEWVPG 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            +VK+KRFHNWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLEKLSG KVFDL
Sbjct: 721  YVKDKRFHNWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRH IDHITIPS RG E+GVLRRV+DVFDCWFESGSMPYAYIHYPFEN ELFEKNFPG F
Sbjct: 781  HRHHIDHITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+++
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVID 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            +YGADA+RLYLINSPVVRAE LRFKKEGV GVV+DVFLPWYNAYRFLVQNAKRLE EG  
Sbjct: 901  EYGADAVRLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGV 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             F P D AT+Q S N+LDQWI+SATQSLV FVR+EMDAYRLYTVVP LLKFLDNLTNIYV
Sbjct: 961  PFVPTDLATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+
Sbjct: 1021 RFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESV 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCS P   G  GERIE SVTRMM IIDLARNIRER+  PLKTPL+EMIVVHPD DFL+D
Sbjct: 1081 HYCSIPPREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLND 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            I G LREYVLEELN+RSL+PCNDTLKYASL+AEPDFS+LGKRLGK+MG+VAKEVK MSQ+
Sbjct: 1141 ITGVLREYVLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQK 1190

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFE+ G VTIA H L+ ++IK+VR F+RPD + + EID++GDGDVLVILDLR D+SL
Sbjct: 1201 DILAFEEAGEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSL 1190

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
             EAG AREIVNRIQKLRKK+ LEPTD VEVYFQSLD+D SV+++VL +QE  I ++IGS 
Sbjct: 1261 VEAGFAREIVNRIQKLRKKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGST 1190

Query: 1321 LLPSAVLPSYAVTLAEESF----------HNVAGISFSIILARPVPVFNSDSITALYEGN 1380
            LL S+++PS+AV +A+E+F            V  +S+ I LARP   FN +++ ALY G+
Sbjct: 1321 LLLSSLMPSHAVIIADETFTPKETSDESVKKVPKLSYKISLARPALKFNEEAVLALYSGD 1190

Query: 1381 EKSARALEVYLLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKA 1434
             KSA  L+ YLLSRD SNLKSEF +G+GKI V  I+  P   VVLGEH+ L+ GD  L  
Sbjct: 1381 VKSATGLQTYLLSRDHSNLKSEFQAGDGKITVSCIENVPVATVVLGEHLHLSVGDDLLSK 1190

BLAST of Cp4.1LG07g05300 vs. ExPASy Swiss-Prot
Match: P41252 (Isoleucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=IARS1 PE=1 SV=2)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 593/1355 (43.76%), Postives = 782/1355 (57.71%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ +FP  EE +LE+W++   F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGI+  +DV KMGI +YN +CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCK 187
            Y  EW+  ++R GRWIDF NDYKT+  +FMESVWWVF QLY+KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACN 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCVNANFIYV 247
            TPLSNFE+ Q+YKDV DP + VTFP LE+    + VAWTTTPWTLPSNLA+CVN    YV
Sbjct: 187  TPLSNFESHQNYKDVQDPSVFVTFP-LEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYV 246

Query: 248  KVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKTENSVEDS 307
            K+++   G++ ++ ++RLSAL                                   +E  
Sbjct: 247  KIKDVARGRLLILMEARLSAL---------------------------------YKLESD 306

Query: 308  FEILEEVLGASLVGKKYEPLFDYF-KEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDD 367
            +EILE   GA L GKKY PLFDYF K   + AF V+ D+YV ++ GTG+VH AP FG +D
Sbjct: 307  YEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAED 366

Query: 368  YRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSDTQCSMF 427
            YRVC++  +I K                                                
Sbjct: 367  YRVCMDFNIIRK------------------------------------------------ 426

Query: 428  QHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTYWVPDFV 487
                                                                        
Sbjct: 427  ------------------------------------------------------------ 486

Query: 488  KEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKSWKIGF 547
                                                                        
Sbjct: 487  ------------------------------------------------------------ 546

Query: 548  AHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENLIVAVDD 607
                           D+LP                                   +  VD 
Sbjct: 547  ---------------DSLP-----------------------------------VCPVDA 606

Query: 608  DGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVPS 667
             GCFTT++TDF+G+YVKDADK II  +K QGRL+ + +FTHSYPFCWRSDTPLIY+AVPS
Sbjct: 607  SGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPS 666

Query: 668  WQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPDFVKEKR 727
            W                              FVRVE + ++LL NN+  YWVP+ V+EKR
Sbjct: 667  W------------------------------FVRVENMVDQLLRNNDLCYWVPELVREKR 726

Query: 728  FHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDLHRHKID 787
            F NWL++ARDW +SR+R+WGTP+P+WVS+D EE++ + S+ +LE+LSGAK+ DLHR  +D
Sbjct: 727  FGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVD 786

Query: 788  HITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLD 847
            H+TIPS+ G   G L R+ +VFDCWFESGSMPYA +HYPFEN   FE  FP  F+AEG+D
Sbjct: 787  HLTIPSRCGK--GSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGID 846

Query: 848  QTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVNDYGADA 907
            QTRGWFYTL+VL+TALFG+P F+N+I NGLVLA DG+KMSKR KNYP P+ I+  YGADA
Sbjct: 847  QTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADA 906

Query: 908  LRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLAQFSPLD 967
            LRLYLINSPVVRAE LRFK+EGV  V++DV LPWYNAYRFL+QN  RL+ E   +F   +
Sbjct: 907  LRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFL-YN 966

Query: 968  QATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYVRFNRKR 1027
            + T+++S N+ D+WI S  QSL+ F   EM AYRLYTVVP L+KF+D LTN YVR NR+R
Sbjct: 967  ENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRR 1026

Query: 1028 LKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESI 1087
            LKG  G EDC +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       V D    SI
Sbjct: 1027 LKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSI 1071

Query: 1088 HYCSYPQEGGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLD 1147
            HY   P+   +   ++ E +V++M ++I+L R IR+R   P+K PL+E++V+H D + L 
Sbjct: 1087 HYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALK 1071

Query: 1148 DIAGKLREYVLEELNIRSLIPCNDTLKYA-SLRAEPDFSLLGKRLGKAMGVVAKEVKAMS 1207
            DI   L +Y++EELN+R +    D  KY   LRAEPD  +LGKRL  A   V   +K +S
Sbjct: 1147 DIK-SLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLS 1071

Query: 1208 QEDILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDE 1267
             E++  F+K G + +  H L   +I+++  F +  G T  + +A  D   LV+LD+ PD+
Sbjct: 1207 SEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGT-AQFEAHSDAQALVLLDVTPDQ 1071

Query: 1268 SLFEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIG 1327
            S+ + G+ARE++NRIQKLRKK  L PTD + VY+++  +  +    V+ +   +I   I 
Sbjct: 1267 SMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEG-TYLNSVIESHTEFIFTTIK 1071

Query: 1328 SPLLPSAVLPSYAVTLAEESFHNVAGISFSIILAR 1354
            +PL P  V PS  V + E++   + G    I L R
Sbjct: 1327 APLKPYPVSPSDKVLIQEKT--QLKGSELEITLTR 1071

BLAST of Cp4.1LG07g05300 vs. ExPASy Swiss-Prot
Match: Q8BU30 (Isoleucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Iars1 PE=1 SV=2)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 598/1340 (44.63%), Postives = 781/1340 (58.28%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ SFP  EE +LE+WS    F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGIK  +DV KMGI +YN++CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCK 187
            Y  EW+  +TR GRWIDF NDYKT+  +FMESVWWVF QLY+KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACG 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCVNANFIYV 247
            TPLSNFE+ Q+YKDV DP + VTFP LE+  N + VAWTTTPWTLPSNLALCVN    YV
Sbjct: 187  TPLSNFESNQNYKDVQDPSVFVTFP-LEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYV 246

Query: 248  KVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKTENSVEDS 307
            K+++   GK+F++ ++RLSAL   KQ                               E  
Sbjct: 247  KIKDVARGKLFILTEARLSAL--YKQ-------------------------------ESD 306

Query: 308  FEILEEVLGASLVGKKYEPLFDYF-KEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDD 367
            +EILE   GASL GKKY+PLFDYF K   + AF V+ D YV D+ GTG+VH AP FG DD
Sbjct: 307  YEILERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADD 366

Query: 368  YRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSDTQCSMF 427
            +RVC++  +I K                                                
Sbjct: 367  HRVCMDFNIIQK------------------------------------------------ 426

Query: 428  QHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTYWVPDFV 487
                                                                        
Sbjct: 427  ------------------------------------------------------------ 486

Query: 488  KEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKSWKIGF 547
                                                     DS+                
Sbjct: 487  -----------------------------------------DSV---------------- 546

Query: 548  AHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENLIVAVDD 607
              P+C                                                    VD 
Sbjct: 547  --PVC---------------------------------------------------PVDA 606

Query: 608  DGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVPS 667
             GCFT ++T F G+YVKDADK+II  +K QGRL+ +G+FTHSYPFCWRSDTPLIY++VPS
Sbjct: 607  SGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTFTHSYPFCWRSDTPLIYKSVPS 666

Query: 668  WQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPDFVKEKR 727
            W                              FVRVE + ++LL+NN+  YWVP+FV+EKR
Sbjct: 667  W------------------------------FVRVEPMVDQLLKNNDLCYWVPEFVREKR 726

Query: 728  FHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDLHRHKID 787
            F NWL+ ARDWA+SR+R+WGTP+P+WVSED EE++ + S+ +LE+LSG K+ DLHR  ID
Sbjct: 727  FGNWLKEARDWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEELSGTKISDLHRESID 786

Query: 788  HITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLD 847
            H+TIPS+ G     LRRV +VFDCWFESGSMPYA +HYPFE+   FE  FP  F+AEG+D
Sbjct: 787  HLTIPSRCGK--APLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGID 846

Query: 848  QTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVNDYGADA 907
            QTRGWFYTL+VL+TALFG+P F+N+I NGL+LA DG+KMSKR KNYP P+ I++ YGADA
Sbjct: 847  QTRGWFYTLLVLATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADA 906

Query: 908  LRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLAQFSPLD 967
            LRLYLINSPVVRAE LRFK+EGV  V++DV LPWYNAYRF +QN  RL  E   +F   +
Sbjct: 907  LRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFL-YN 966

Query: 968  QATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYVRFNRKR 1027
            + T+++S N+ D+W+ S  QSL+ F   EM AYRLYTVVP L+KF+D LTN YVR NR+R
Sbjct: 967  EHTVRESPNITDRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRR 1026

Query: 1028 LKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESI 1087
            LKG +G EDC +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       + D    SI
Sbjct: 1027 LKGESGVEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDTLSI 1058

Query: 1088 HYCSYPQEGGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLD 1147
            HY   P+   +   ++ E +V+RM ++I+L R IR+R   P+K PL+E++V+H D + L+
Sbjct: 1087 HYLMLPRVREELIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALE 1058

Query: 1148 DIAGKLREYVLEELNIRSLIPCNDTLKYA-SLRAEPDFSLLGKRLGKAMGVVAKEVKAMS 1207
            DI   L +Y++EELN+R +    D  KY   LRAEPD  +LGKRL  A   V   +K +S
Sbjct: 1147 DIR-SLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMMAIKRLS 1058

Query: 1208 QEDILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDE 1267
             E++  F+K G++ +  H L   +I+++  F +  G T  + +A  D   LV+LD+ PD+
Sbjct: 1207 NEELERFQKSGSIVVEGHELHEEDIRLMYTFDQATGGT-AQFEAHSDAQALVLLDVTPDQ 1058

Query: 1268 SLFEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIG 1327
            S+ + G+ARE++NRIQKLRKK  L PTD + VY+ +  +       V+ +   +I   I 
Sbjct: 1267 SMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSEG-RYLNNVIESHTDFIFATIK 1058

Query: 1328 SPLLPSAVLPSYAVTLAEES 1339
            +PL P  V  S  + + E++
Sbjct: 1327 APLKPYPVPTSDNILIQEQT 1058

BLAST of Cp4.1LG07g05300 vs. ExPASy Swiss-Prot
Match: Q54YD4 (Probable isoleucine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=ileS PE=3 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 578/1323 (43.69%), Postives = 760/1323 (57.45%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EV +  DF+    E  +L+YW DIKAFET ++ +   PEY FYDGPPFATGLPHYGHI
Sbjct: 1    MEEVPQKIDFA--GEESKILKYWDDIKAFETSVKMSEGKPEYSFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKD +TRY   TGHHV RRFGWDCHGLP+E EID+  G++ ++DVLKMGI  YN+ 
Sbjct: 61   LAGTIKDTITRYAHQTGHHVERRFGWDCHGLPIEFEIDKLHGVRTKEDVLKMGIPTYNQH 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIV +Y  EWE ++ R GRWID KN+YKTMD  FMESVWWVF +L++K LVY+GFKVM
Sbjct: 121  CRSIVMKYSHEWEIVVNRMGRWIDMKNNYKTMDTPFMESVWWVFQELFKKDLVYQGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYS GC TPLSNFEA  +YKDV DP  +V+F  L+D    + +AWTTTPWTLPSNLAL V
Sbjct: 181  PYSIGCTTPLSNFEASSNYKDVSDPACVVSFQTLDD-EKVSILAWTTTPWTLPSNLALTV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            N    Y+K+ +    ++F++ K+R S L                                
Sbjct: 241  NPKMEYIKINDIKRNQIFILGKNRTSIL-------------------------------- 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDV-AFRVVADSYVTDDSGTGIVHCA 360
                +  + +LE + G  L+GKKY P+F YF   +++  F V+   +VTDDSGTGIVH A
Sbjct: 301  -YKSDKEYTVLETMKGTDLIGKKYVPMFPYFASDANMGGFVVIGGDFVTDDSGTGIVHTA 360

Query: 361  PAFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCS 420
            PA+GEDD+ VCI N VI++                                         
Sbjct: 361  PAYGEDDFNVCIANGVISRD---------------------------------------- 420

Query: 421  DTQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKT 480
                   Q K  +LN                                             
Sbjct: 421  -------QFKRPILN--------------------------------------------- 480

Query: 481  YWVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGA 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  KSWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGEN 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  LIVAVDDDGCFTTKITDFSGRYVKDAD--KDIIEAVKAQGRLVKSGSFTHSYPFCWRSDT 660
               +VD +GCFT+ +TDF+G  VKDA+  K I   +K +GR+V S +  HSYP+CWRSDT
Sbjct: 601  ---SVDANGCFTSDVTDFAGMMVKDAETTKQISIYLKNKGRMVNSANLVHSYPYCWRSDT 660

Query: 661  PLIYRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYW 720
            PLIY+AV SW                              FVRVE +++KLL NN+KTYW
Sbjct: 661  PLIYKAVGSW------------------------------FVRVESIRDKLLANNDKTYW 720

Query: 721  VPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKV 780
            VPDFVKEKRF NWL+NA DWAVSR+R+WGTP+P+W+SEDGEE++V+ SI++LE+LSG +V
Sbjct: 721  VPDFVKEKRFANWLKNATDWAVSRNRYWGTPIPLWISEDGEEVVVIGSIDELERLSGVRV 780

Query: 781  FDLHRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFP 840
             DLHR  IDHITIPS++G   G LRR++DVFDCWFESGSMPYA  HYPFEN + FEK FP
Sbjct: 781  TDLHRESIDHITIPSQKGK--GTLRRIEDVFDCWFESGSMPYAQQHYPFENKDKFEKIFP 840

Query: 841  GHFVAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPME 900
             HF+AEGLDQTRGWFYTL+VLSTALF KP F+NLI NGLVLA DGKKMSKRLKNYP PME
Sbjct: 841  AHFIAEGLDQTRGWFYTLLVLSTALFDKPPFQNLIVNGLVLAADGKKMSKRLKNYPDPME 900

Query: 901  IVNDYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIE 960
            +V+  GAD+LRLYLINSPVVRAETL+F+++GV  +++DVFLPW+NAYRF VQN  R E  
Sbjct: 901  VVSKVGADSLRLYLINSPVVRAETLKFQEKGVQDMIKDVFLPWFNAYRFFVQNCLRFEKA 960

Query: 961  GLAQFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTN 1020
                F P D      S NV+D+WI ++ QSL+ FVR EM AYRLYTVVP L++F+++LTN
Sbjct: 961  TNTTFQP-DIKVALASENVMDKWILASCQSLIAFVRAEMAAYRLYTVVPKLVRFIEDLTN 1020

Query: 1021 IYVRFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKV--SDG 1080
             +VR NRKRLKG  G+ DC  AL+ LY VL+T C  M PFTPFFTE +YQNL+K    + 
Sbjct: 1021 WFVRLNRKRLKGSNGDADCLAALNILYEVLMTICIAMGPFTPFFTEYMYQNLKKALPKEK 1031

Query: 1081 SEESIHYCSYPQEGGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPD 1140
              +S+HY  +P+   +    RIEE+++RM  +I+L R  R+R  KP+K PL+E +V+  +
Sbjct: 1081 QMDSVHYVMFPEPIQEAFNTRIEEAISRMQVVIELGRAARDRRTKPIKFPLKEFMVITEN 1031

Query: 1141 KDFLDDIAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEV 1200
            + +L D+   L+ Y+LEELNI++++  +D   +  + AE D   LG RL      ++  +
Sbjct: 1141 QQYLADLE-SLKSYILEELNIQNIVLTSDEGSFVVVTAEADNKRLGARLKNDFKKISPLI 1031

Query: 1201 KAMSQEDILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDL 1260
              ++ E +  F+K   + I  H L   ++K++R +   +G T    + SG+ ++L +LDL
Sbjct: 1201 SKLTNEQLREFQKTQTIDILGHELTSEDLKIIRKY---NGETTNS-EPSGNEEILTVLDL 1031

Query: 1261 RPDESLFEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYIS 1318
              D +L+E G+ARE++NR+Q+LRKK+ L   D V +++ + + ++  A   +     YI 
Sbjct: 1261 TVDSALYEKGLARELINRVQRLRKKSGLTFDDPVSMFYHTKEAELKTA---IENNNDYIK 1031

BLAST of Cp4.1LG07g05300 vs. ExPASy Swiss-Prot
Match: Q21926 (Isoleucine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=iars-1 PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 1.3e-292
Identity = 566/1318 (42.94%), Postives = 720/1318 (54.63%), Query Frame = 0

Query: 9    DFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDI 68
            + +F + E+ V + W D   F+  +E ++D P + FYDGPPFATGLPHYGH+L  TIKD+
Sbjct: 10   NINFAREEDKVAQKWQDENTFQRSVELSKDRPHFTFYDGPPFATGLPHYGHMLTSTIKDV 69

Query: 69   VTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEECRSIVTRY 128
            V R+    GH+V RRFGWD HGLPVE EID+ LGI    DV+KMGI  YN ECR IV RY
Sbjct: 70   VGRWAHQNGHYVERRFGWDTHGLPVEYEIDKTLGISGPQDVMKMGIANYNNECRKIVMRY 129

Query: 129  VGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKT 188
             GEWEK + R GRW+DFK+DYKT+   FMESVWW FS+L++KGLVYKG KVMP+ST C T
Sbjct: 130  SGEWEKTMGRLGRWVDFKHDYKTLYPWFMESVWWAFSELHKKGLVYKGVKVMPFSTACST 189

Query: 189  PLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCVNANFIYVK 248
            PLSNFEAGQ+YKDV DP + V F +L+ P N   VAWTTTPWTLPSNLAL V+ + +YV 
Sbjct: 190  PLSNFEAGQNYKDVVDPAVFVGFKLLDCP-NRQLVAWTTTPWTLPSNLALVVHPDMLYVV 249

Query: 249  VRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKTENSVEDSF 308
             ++K +G  +VV + RL  L                                     D+ 
Sbjct: 250  TKDKTTGIEYVVLEERLGELK-----------------------------------NDNL 309

Query: 309  EILEEVLGASLVGKKYEPLFDYFKEFSDV--AFRVVADSYVTDDSGTGIVHCAPAFGEDD 368
            E++E++ G+ L   +YEPLF YF    +   AFRV+ D++VT DSGTG+VH AP FGE D
Sbjct: 310  EVIEKLAGSQLKDLRYEPLFPYFAYMREERNAFRVLNDTFVTSDSGTGVVHQAPYFGEID 369

Query: 369  YRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSDTQCSMF 428
            ++VC+ N VI K + +I  VD+           SG+Y                       
Sbjct: 370  FQVCVANGVIAKDQKMICPVDE-----------SGKY----------------------- 429

Query: 429  QHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTYWVPDFV 488
                                                                        
Sbjct: 430  ------------------------------------------------------------ 489

Query: 489  KEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKSWKIGF 548
                                                                        
Sbjct: 490  ------------------------------------------------------------ 549

Query: 549  AHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENLIVAVDD 608
                                                                        
Sbjct: 550  ------------------------------------------------------------ 609

Query: 609  DGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVPS 668
                T+++ D+ G YVKDADK II+ +K  G LV+     HSYPFCWRSDTPL+Y+AVPS
Sbjct: 610  ----TSEVPDYQGVYVKDADKLIIKRLKEMGNLVRQAEVKHSYPFCWRSDTPLLYKAVPS 669

Query: 669  WQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPDFVKEKR 728
            W                              F+ VE L  +LL NN++TYWVP FVKEKR
Sbjct: 670  W------------------------------FINVETLIPRLLANNDETYWVPAFVKEKR 729

Query: 729  FHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDLHRHKID 788
            F NWL +ARDWAVSR+RFWGTP+ +WVSEDGEE++ + SI +LE+LSG K+ DLHR  +D
Sbjct: 730  FANWLRDARDWAVSRNRFWGTPINLWVSEDGEEVVCVGSIAELEELSGQKITDLHRESVD 789

Query: 789  HITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLD 848
             +TIPS+ G   GVL+RV +VFDCWFESGSMPYA  HYPFEN ++FE NFP  F+AEG+D
Sbjct: 790  DVTIPSRSG--RGVLKRVSEVFDCWFESGSMPYAQNHYPFENRKIFEDNFPADFIAEGID 849

Query: 849  QTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVNDYGADA 908
            QTRGWFYTL+VLSTALF KP F+NLICNGLVLA DG KMSK  KNYP PM IVN YGADA
Sbjct: 850  QTRGWFYTLLVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADA 909

Query: 909  LRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLAQFSPLD 968
            LRLYLINSPVVR E LRF++EGV  +++DVFLPW+NAYRF VQN +  E E    F   D
Sbjct: 910  LRLYLINSPVVRGENLRFREEGVRDLLKDVFLPWFNAYRFFVQNVQAYEHETGNVF---D 969

Query: 969  QATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYVRFNRKR 1028
                  S NV+D+WI S T SLV FVRKEMD+YRLY VV  L KF D LTNIY+R NRKR
Sbjct: 970  MNVHVASENVMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTKFFDTLTNIYIRLNRKR 1029

Query: 1029 LKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYP 1088
            +KG  G  +   AL+ L  VL+   ++MAPFTPFF E ++ NL+KV   +EES+H+   P
Sbjct: 1030 VKGDNGLHEQHHALAALGRVLILIVRLMAPFTPFFCEYIWLNLKKVIGSTEESVHFLMLP 1033

Query: 1089 Q-EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKL 1148
            + +     E +E  V  M  +IDL R +R+R    +K PL+EMIV++ D  FL+D+   L
Sbjct: 1090 KPDESLIDETVERRVEVMRNVIDLVRLVRDREGLAVKYPLKEMIVINRDSQFLEDVK-SL 1033

Query: 1149 REYVLEELNIRSLIPCNDTLKYA-SLRAEPDFSLLGKRLGKAMGVVAKEVK-AMSQEDIL 1208
              Y+L ELN+R L    D  KY  +L+AEP+F +LG RL      VA  +K  +++ ++ 
Sbjct: 1150 ESYILLELNVRKLTVSQDKQKYGITLKAEPNFKILGARLKGEQKKVADYLKNQITESELE 1033

Query: 1209 AFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESLFEA 1268
             F   G +T+  H L   E+ V        G   K      D   +V++D   DESL E 
Sbjct: 1210 KFLLEGKLTVLGHELSSEEVAVSYAAGSDQGHGYK---THSDAKTIVMIDTTEDESLIEE 1033

Query: 1269 GVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSPL 1322
            G+ RE+ NR+Q+LRK+A L  TDT  V+      D  +A +V+ A+   I  A G+P+
Sbjct: 1270 GLCREVTNRVQRLRKQAKLVSTDTAHVHIVVSPADSQLA-KVVAAKLNDIVSATGTPI 1033

BLAST of Cp4.1LG07g05300 vs. NCBI nr
Match: XP_023537189.1 (isoleucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2292 bits (5939), Expect = 0.0
Identity = 1184/1433 (82.62%), Postives = 1185/1433 (82.69%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKEKLLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKEKLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPS+RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSQRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
            QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1185

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1185

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1185

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY
Sbjct: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1185

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
            LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS
Sbjct: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1185

BLAST of Cp4.1LG07g05300 vs. NCBI nr
Match: XP_022951257.1 (isoleucine--tRNA ligase, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 2284 bits (5918), Expect = 0.0
Identity = 1179/1433 (82.27%), Postives = 1183/1433 (82.55%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKV+APNGPTDVSKKSNPKTKGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVSAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKE SDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEVSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKEKLLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKEKLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
            +FSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  RFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1185

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1185

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+SLDDDISVAERVLRAQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSLDDDISVAERVLRAQELYISEAIGSP 1185

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY
Sbjct: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1185

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
             LSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS
Sbjct: 1381 FLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1185

BLAST of Cp4.1LG07g05300 vs. NCBI nr
Match: XP_023001832.1 (isoleucine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 2263 bits (5864), Expect = 0.0
Identity = 1168/1423 (82.08%), Postives = 1172/1423 (82.36%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKV APNGPTDVSKKSNPKTKGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVKAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGK+YEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKRYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDII+AVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIDAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKEKLLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKEKLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAE LRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA
Sbjct: 901  DYGADALRLYLINSPVVRAEILRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
            +FSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  RFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYP EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPLEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1175

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1175

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1175

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY
Sbjct: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1175

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTA 1423
            LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTA
Sbjct: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTA 1175

BLAST of Cp4.1LG07g05300 vs. NCBI nr
Match: XP_038898074.1 (isoleucine--tRNA ligase, cytoplasmic isoform X2 [Benincasa hispida])

HSP 1 Score: 2212 bits (5731), Expect = 0.0
Identity = 1144/1433 (79.83%), Postives = 1159/1433 (80.88%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNAPN    V  KSNPK KGSSNSKT
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDSV--KSNPKKKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKY PLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKEKLLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKEKLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSG KVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVE FEKNFPGHF
Sbjct: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVEHFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             FSP+DQATLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVH DKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHSDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLI C+DTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLITCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQE 1183

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVG VTIATHCLRLSEIKVVRDFRRPDGMTDKEIDA+GDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGEVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1183

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+S D+DISVA+RVLRAQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFKSQDEDISVAQRVLRAQELYISEAIGSP 1183

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPS VLPSYAVTLAEESFHNVAGISFSIILARP   FNSD+ITALYEGNEK ARALEVY
Sbjct: 1321 LLPSTVLPSYAVTLAEESFHNVAGISFSIILARPALAFNSDAITALYEGNEKFARALEVY 1183

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
            LLSRDL+NLKSEFSSGNGKIRVDFIDGQP VEV+LGEHVFLTAGDQFLKAK+S
Sbjct: 1381 LLSRDLTNLKSEFSSGNGKIRVDFIDGQPSVEVLLGEHVFLTAGDQFLKAKAS 1183

BLAST of Cp4.1LG07g05300 vs. NCBI nr
Match: XP_008453992.2 (PREDICTED: LOW QUALITY PROTEIN: isoleucine--tRNA ligase, cytoplasmic-like [Cucumis melo])

HSP 1 Score: 2210 bits (5727), Expect = 0.0
Identity = 1140/1433 (79.55%), Postives = 1158/1433 (80.81%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNAPN   D   KSNPK KGSSNSKT
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNA-NDGINKSNPKKKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKY PLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKE LLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKENLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSG KVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPS+RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             FSP+DQATLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQE 1184

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVG +TIATHCLRLSEIKVVRDF+RPDGMTDKEIDA+GDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGEITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1184

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+S D+D SVA RVL+AQ+LYISEAIGS 
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSX 1184

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPS VLPSYAVTLAEESFHNVAGISFSIILARP PVF+SD+ITALYEGNEK ARALEVY
Sbjct: 1321 LLPSTVLPSYAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVY 1184

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
            LLSRDLSNLKSEFSSGNGKIRVDFIDGQP VEVVLGEHVFLTAGDQ+LKA SS
Sbjct: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1184

BLAST of Cp4.1LG07g05300 vs. ExPASy TrEMBL
Match: A0A6J1GH53 (Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111454148 PE=3 SV=1)

HSP 1 Score: 2284 bits (5918), Expect = 0.0
Identity = 1179/1433 (82.27%), Postives = 1183/1433 (82.55%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKV+APNGPTDVSKKSNPKTKGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVSAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKE SDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEVSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKEKLLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKEKLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
            +FSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  RFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1185

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1185

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+SLDDDISVAERVLRAQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSLDDDISVAERVLRAQELYISEAIGSP 1185

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY
Sbjct: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1185

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
             LSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS
Sbjct: 1381 FLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1185

BLAST of Cp4.1LG07g05300 vs. ExPASy TrEMBL
Match: A0A6J1KHQ9 (Isoleucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111495907 PE=3 SV=1)

HSP 1 Score: 2263 bits (5864), Expect = 0.0
Identity = 1168/1423 (82.08%), Postives = 1172/1423 (82.36%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKV APNGPTDVSKKSNPKTKGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVKAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGK+YEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKRYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDII+AVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIDAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKEKLLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKEKLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAE LRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA
Sbjct: 901  DYGADALRLYLINSPVVRAEILRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
            +FSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  RFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYP EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPLEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1175

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1175

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1175

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY
Sbjct: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1175

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTA 1423
            LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTA
Sbjct: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTA 1175

BLAST of Cp4.1LG07g05300 vs. ExPASy TrEMBL
Match: A0A1S3BX29 (Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494549 PE=3 SV=1)

HSP 1 Score: 2210 bits (5727), Expect = 0.0
Identity = 1140/1433 (79.55%), Postives = 1158/1433 (80.81%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNAPN   D   KSNPK KGSSNSKT
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNA-NDGINKSNPKKKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKY PLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKE LLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKENLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSG KVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPS+RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             FSP+DQATLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQE 1184

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVG +TIATHCLRLSEIKVVRDF+RPDGMTDKEIDA+GDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGEITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1184

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+S D+D SVA RVL+AQ+LYISEAIGS 
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAHRVLQAQDLYISEAIGSX 1184

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPS VLPSYAVTLAEESFHNVAGISFSIILARP PVF+SD+ITALYEGNEK ARALEVY
Sbjct: 1321 LLPSTVLPSYAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVY 1184

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
            LLSRDLSNLKSEFSSGNGKIRVDFIDGQP VEVVLGEHVFLTAGDQ+LKA SS
Sbjct: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1184

BLAST of Cp4.1LG07g05300 vs. ExPASy TrEMBL
Match: A0A0A0KTX5 (Isoleucyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G013540 PE=3 SV=1)

HSP 1 Score: 2205 bits (5714), Expect = 0.0
Identity = 1137/1433 (79.34%), Postives = 1157/1433 (80.74%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNA N   D   KSNPK KGSSNSKT
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANA-NDGINKSNPKKKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKY PLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCF +KITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVEKLKE LLENNEKTYWVPD
Sbjct: 661  YRAVPSW------------------------------FVRVEKLKENLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSG KVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPS+RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             FSP+DQATLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQE 1184

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVG VTIATHCL+LSEIKVVRDF+RPDGMTDKEIDA+GDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGEVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1184

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+S D+D SVA+RVL+AQELYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSQDEDTSVAQRVLQAQELYISEAIGSP 1184

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPS VLPSYAVTLAEESFHNVAGISFSIILARP PVFNSD+ITALYEGNEK AR+LEVY
Sbjct: 1321 LLPSTVLPSYAVTLAEESFHNVAGISFSIILARPAPVFNSDAITALYEGNEKFARSLEVY 1184

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
            LLSRDLSNLKSEFS+GNGKIRVDFIDGQP VEVVL EHVFLTAGDQ+LKA SS
Sbjct: 1381 LLSRDLSNLKSEFSNGNGKIRVDFIDGQPSVEVVLAEHVFLTAGDQYLKANSS 1184

BLAST of Cp4.1LG07g05300 vs. ExPASy TrEMBL
Match: A0A5A7TMQ4 (Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003320 PE=3 SV=1)

HSP 1 Score: 2203 bits (5709), Expect = 0.0
Identity = 1136/1433 (79.27%), Postives = 1155/1433 (80.60%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNAPN   D   KSNPK KGSSNSKT
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNA-NDGINKSNPKKKGSSNSKT 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            ENSVEDSFEILEEVLGASLVGKKY PLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDA             
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDA------------- 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
                                     DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI
Sbjct: 601  -------------------------DKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSWQ                                  KLKE LLENNEKTYWVPD
Sbjct: 661  YRAVPSWQ----------------------------------KLKENLLENNEKTYWVPD 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSG KVFDL
Sbjct: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRHKIDHITIPS+RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF
Sbjct: 781  HRHKIDHITIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+N
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIN 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEG A
Sbjct: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFA 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             FSP+DQATLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYV
Sbjct: 961  PFSPVDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI
Sbjct: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PLREMIVVHPDKDFLDD
Sbjct: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDD 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFS+LGKRLGKAMGVVAKEVKAMSQE
Sbjct: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQE 1180

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFEKVG +TIATHCLRLSEIKVVRDF+RPDGMTDKEIDA+GDGDVLVILDLRPDESL
Sbjct: 1201 DILAFEKVGEITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESL 1180

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
            FEAGVAREIVNRIQKLRKKAALEPTDTVEVYF+S D++ SVA RVL+AQ+LYISEAIGSP
Sbjct: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFRSQDENTSVAHRVLQAQDLYISEAIGSP 1180

Query: 1321 LLPSAVLPSYAVTLAEESFHNVAGISFSIILARPVPVFNSDSITALYEGNEKSARALEVY 1380
            LLPS VLPSYAVTLAEESFHNVAGISFSIILARP PVF+SD+ITALYEGNEK ARALEVY
Sbjct: 1321 LLPSTVLPSYAVTLAEESFHNVAGISFSIILARPAPVFDSDAITALYEGNEKFARALEVY 1180

Query: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKAKSS 1433
            LLSRDLSNLKSEFSSGNGKIRVDFIDGQP VEVVLGEHVFLTAGDQ+LKA SS
Sbjct: 1381 LLSRDLSNLKSEFSSGNGKIRVDFIDGQPSVEVVLGEHVFLTAGDQYLKANSS 1180

BLAST of Cp4.1LG07g05300 vs. TAIR 10
Match: AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 1800.4 bits (4662), Expect = 0.0e+00
Identity = 917/1443 (63.55%), Postives = 1042/1443 (72.21%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL+EK LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMVTFPV+ D  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300
            NA F+YVKVRNK +GKV++VA+SRLSALP +K K    NGP   +KK+NPK KG+     
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDTKKANPKAKGAK---- 300

Query: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
              S  DS+E+LE+  GASLVGKKYEPLFDYF +FS  AFRVVAD YVTDDSGTGIVHCAP
Sbjct: 301  PESAADSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSD 420
            AFGEDDYRVC+ N++I K                                          
Sbjct: 361  AFGEDDYRVCLLNKIIKK------------------------------------------ 420

Query: 421  TQCSMFQHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTY 480
                                                                        
Sbjct: 421  ------------------------------------------------------------ 480

Query: 481  WVPDFVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAK 540
                                                                        
Sbjct: 481  ------------------------------------------------------------ 540

Query: 541  SWKIGFAHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENL 600
                                                                    GENL
Sbjct: 541  --------------------------------------------------------GENL 600

Query: 601  IVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLI 660
            +VAVDDDG FT +IT FSGRYVKDADKDIIEAVKA+GRLVK+GSFTHSYPFCWRSDTPLI
Sbjct: 601  VVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKGRLVKTGSFTHSYPFCWRSDTPLI 660

Query: 661  YRAVPSWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPD 720
            YRAVPSW                              FVRVE+LKEKLL++NE+T WVP 
Sbjct: 661  YRAVPSW------------------------------FVRVEQLKEKLLKSNEQTEWVPG 720

Query: 721  FVKEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDL 780
            +VK+KRFHNWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLEKLSG KVFDL
Sbjct: 721  YVKDKRFHNWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDL 780

Query: 781  HRHKIDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHF 840
            HRH IDHITIPS RG E+GVLRRV+DVFDCWFESGSMPYAYIHYPFEN ELFEKNFPG F
Sbjct: 781  HRHHIDHITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDF 840

Query: 841  VAEGLDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVN 900
            VAEGLDQTRGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+++
Sbjct: 841  VAEGLDQTRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVID 900

Query: 901  DYGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLA 960
            +YGADA+RLYLINSPVVRAE LRFKKEGV GVV+DVFLPWYNAYRFLVQNAKRLE EG  
Sbjct: 901  EYGADAVRLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGV 960

Query: 961  QFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYV 1020
             F P D AT+Q S N+LDQWI+SATQSLV FVR+EMDAYRLYTVVP LLKFLDNLTNIYV
Sbjct: 961  PFVPTDLATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYV 1020

Query: 1021 RFNRKRLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESI 1080
            RFNRKRLKGRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+
Sbjct: 1021 RFNRKRLKGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESV 1080

Query: 1081 HYCSYPQEGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDD 1140
            HYCS P   G  GERIE SVTRMM IIDLARNIRER+  PLKTPL+EMIVVHPD DFL+D
Sbjct: 1081 HYCSIPPREGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLND 1140

Query: 1141 IAGKLREYVLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQE 1200
            I G LREYVLEELN+RSL+PCNDTLKYASL+AEPDFS+LGKRLGK+MG+VAKEVK MSQ+
Sbjct: 1141 ITGVLREYVLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQK 1190

Query: 1201 DILAFEKVGNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESL 1260
            DILAFE+ G VTIA H L+ ++IK+VR F+RPD + + EID++GDGDVLVILDLR D+SL
Sbjct: 1201 DILAFEEAGEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSL 1190

Query: 1261 FEAGVAREIVNRIQKLRKKAALEPTDTVEVYFQSLDDDISVAERVLRAQELYISEAIGSP 1320
             EAG AREIVNRIQKLRKK+ LEPTD VEVYFQSLD+D SV+++VL +QE  I ++IGS 
Sbjct: 1261 VEAGFAREIVNRIQKLRKKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGST 1190

Query: 1321 LLPSAVLPSYAVTLAEESF----------HNVAGISFSIILARPVPVFNSDSITALYEGN 1380
            LL S+++PS+AV +A+E+F            V  +S+ I LARP   FN +++ ALY G+
Sbjct: 1321 LLLSSLMPSHAVIIADETFTPKETSDESVKKVPKLSYKISLARPALKFNEEAVLALYSGD 1190

Query: 1381 EKSARALEVYLLSRDLSNLKSEFSSGNGKIRVDFIDGQPGVEVVLGEHVFLTAGDQFLKA 1434
             KSA  L+ YLLSRD SNLKSEF +G+GKI V  I+  P   VVLGEH+ L+ GD  L  
Sbjct: 1381 VKSATGLQTYLLSRDHSNLKSEFQAGDGKITVSCIENVPVATVVLGEHLHLSVGDDLLSK 1190

BLAST of Cp4.1LG07g05300 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-49
Identity = 237/1133 (20.92%), Postives = 390/1133 (34.42%), Query Frame = 0

Query: 16   EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
            E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123  EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76   TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEECRSIVTRYVGEWEKI 135
              + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183  QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136  ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
              R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196  GQSYKDVPDPEIMVTFPVL---------EDPHNAAFVAWTTTPWTLPSNLALCVNANFIY 255
                  +    I   F ++         E   N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303  EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256  VKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKTENSVED 315
              V  +   +      S    +P +  K N       V+    P  +     K       
Sbjct: 363  SVVEVQSFSEDESTVTSNKKKIPGKVLK-NQQKLFVIVATDLVPALEAKWGVK------- 422

Query: 316  SFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDD 375
               I +  LG+ L   +Y    D     +     V+   Y+T +SGTG+VH AP  G++D
Sbjct: 423  -LSISKTFLGSDLENCRYTHPID-----NRDCPVVIGGDYITTESGTGLVHTAPGHGQED 482

Query: 376  YRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSDTQCSMF 435
            Y                                                           
Sbjct: 483  Y----------------------------------------------------------- 542

Query: 436  QHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTYWVPDFV 495
                                      +TGL                              
Sbjct: 543  --------------------------ATGLK----------------------------- 602

Query: 496  KEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKSWKIGF 555
                                  +G P                                  
Sbjct: 603  ----------------------YGLP---------------------------------- 662

Query: 556  AHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENLIVAVDD 615
                                                                 L+  VDD
Sbjct: 663  -----------------------------------------------------LVSPVDD 722

Query: 616  DGCFTTKITDFSGRYV-KDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVP 675
            +G FT +   F G  V  + +  ++  +     LV   S+ H YP+ WR+  P I+RA  
Sbjct: 723  EGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATE 782

Query: 676  SWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPDFVKEK 735
             W                              F  VE  +   ++      WVP      
Sbjct: 783  QW------------------------------FASVEGFRTATMDAINNVKWVP-HQAVN 842

Query: 736  RFHNWLENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEKLSGAKVFDLHRHK 795
            R      +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   + 
Sbjct: 843  RISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYM 902

Query: 796  IDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEG 855
                 +P K   +     +  D  D WF+SGS  +A +    E +     +FP     EG
Sbjct: 903  SVEDLLPEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEG 962

Query: 856  LDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVND--- 915
             DQ RGWF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  ++     
Sbjct: 963  TDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKN 964

Query: 916  ------YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLE 975
                  YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     
Sbjct: 1023 SKDAPAYGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWR 964

Query: 976  IEGLAQFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKF-LDN 1035
            +         D A   +   ++DQ      +++V  +++  + Y+ + +   + +F + +
Sbjct: 1083 V---------DNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVD 964

Query: 1036 LTNIYVRFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL---R 1095
            L+N Y    + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL    
Sbjct: 1143 LSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEY 964

Query: 1096 KVSDGSEESI-----------HYCSYPQEGGKRGERIEESVTRMMTIIDLARN 1113
            +  DGS                + S+P E     +R+ E  T +  +++LARN
Sbjct: 1203 RNEDGSAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARN 964

BLAST of Cp4.1LG07g05300 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-49
Identity = 237/1133 (20.92%), Postives = 390/1133 (34.42%), Query Frame = 0

Query: 16   EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
            E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 309  EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 368

Query: 76   TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEECRSIVTRYVGEWEKI 135
              + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 369  QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 428

Query: 136  ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
              R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 429  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 488

Query: 196  GQSYKDVPDPEIMVTFPVL---------EDPHNAAFVAWTTTPWTLPSNLALCVNANFIY 255
                  +    I   F ++         E   N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 489  EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 548

Query: 256  VKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKTENSVED 315
              V  +   +      S    +P +  K N       V+    P  +     K       
Sbjct: 549  SVVEVQSFSEDESTVTSNKKKIPGKVLK-NQQKLFVIVATDLVPALEAKWGVK------- 608

Query: 316  SFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDD 375
               I +  LG+ L   +Y    D     +     V+   Y+T +SGTG+VH AP  G++D
Sbjct: 609  -LSISKTFLGSDLENCRYTHPID-----NRDCPVVIGGDYITTESGTGLVHTAPGHGQED 668

Query: 376  YRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSDTQCSMF 435
            Y                                                           
Sbjct: 669  Y----------------------------------------------------------- 728

Query: 436  QHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTYWVPDFV 495
                                      +TGL                              
Sbjct: 729  --------------------------ATGLK----------------------------- 788

Query: 496  KEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKSWKIGF 555
                                  +G P                                  
Sbjct: 789  ----------------------YGLP---------------------------------- 848

Query: 556  AHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENLIVAVDD 615
                                                                 L+  VDD
Sbjct: 849  -----------------------------------------------------LVSPVDD 908

Query: 616  DGCFTTKITDFSGRYV-KDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVP 675
            +G FT +   F G  V  + +  ++  +     LV   S+ H YP+ WR+  P I+RA  
Sbjct: 909  EGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATE 968

Query: 676  SWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPDFVKEK 735
             W                              F  VE  +   ++      WVP      
Sbjct: 969  QW------------------------------FASVEGFRTATMDAINNVKWVP-HQAVN 1028

Query: 736  RFHNWLENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEKLSGAKVFDLHRHK 795
            R      +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   + 
Sbjct: 1029 RISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYM 1088

Query: 796  IDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEG 855
                 +P K   +     +  D  D WF+SGS  +A +    E +     +FP     EG
Sbjct: 1089 SVEDLLPEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEG 1148

Query: 856  LDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVND--- 915
             DQ RGWF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  ++     
Sbjct: 1149 TDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKN 1150

Query: 916  ------YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLE 975
                  YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     
Sbjct: 1209 SKDAPAYGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWR 1150

Query: 976  IEGLAQFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKF-LDN 1035
            +         D A   +   ++DQ      +++V  +++  + Y+ + +   + +F + +
Sbjct: 1269 V---------DNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVD 1150

Query: 1036 LTNIYVRFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL---R 1095
            L+N Y    + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL    
Sbjct: 1329 LSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEY 1150

Query: 1096 KVSDGSEESI-----------HYCSYPQEGGKRGERIEESVTRMMTIIDLARN 1113
            +  DGS                + S+P E     +R+ E  T +  +++LARN
Sbjct: 1389 RNEDGSAAEFVFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARN 1150

BLAST of Cp4.1LG07g05300 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 189.5 bits (480), Expect = 1.9e-47
Identity = 224/1076 (20.82%), Postives = 368/1076 (34.20%), Query Frame = 0

Query: 16   EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
            E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123  EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76   TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEECRSIVTRYVGEWEKI 135
              + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183  QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136  ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
              R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196  GQSYKDVPDPEIMVTFPVL---------EDPHNAAFVAWTTTPWTLPSNLALCVNANFIY 255
                  +    I   F ++         E   N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303  EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256  VKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKTENSVED 315
              V  +   +      S    +P +  K N       V+    P  +     K       
Sbjct: 363  SVVEVQSFSEDESTVTSNKKKIPGKVLK-NQQKLFVIVATDLVPALEAKWGVK------- 422

Query: 316  SFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDD 375
               I +  LG+ L   +Y    D     +     V+   Y+T +SGTG+VH AP  G++D
Sbjct: 423  -LSISKTFLGSDLENCRYTHPID-----NRDCPVVIGGDYITTESGTGLVHTAPGHGQED 482

Query: 376  YRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKVCSDTQCSMF 435
            Y                                                           
Sbjct: 483  Y----------------------------------------------------------- 542

Query: 436  QHKGGLLNREASPILIPSAGDLILPLSTGLSQAGKFVRVEKLKEKLLENNEKTYWVPDFV 495
                                      +TGL                              
Sbjct: 543  --------------------------ATGLK----------------------------- 602

Query: 496  KEKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKSWKIGF 555
                                  +G P                                  
Sbjct: 603  ----------------------YGLP---------------------------------- 662

Query: 556  AHPLCTMFLPTGFDCDALPPVFDLHRHKIDHITIPSQRGPEYGVLRRVDDGENLIVAVDD 615
                                                                 L+  VDD
Sbjct: 663  -----------------------------------------------------LVSPVDD 722

Query: 616  DGCFTTKITDFSGRYV-KDADKDIIEAVKAQGRLVKSGSFTHSYPFCWRSDTPLIYRAVP 675
            +G FT +   F G  V  + +  ++  +     LV   S+ H YP+ WR+  P I+RA  
Sbjct: 723  EGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATE 782

Query: 676  SWQVHLFIYFEICYMLLCLGPYLNFVGVFAHVFVRVEKLKEKLLENNEKTYWVPDFVKEK 735
             W                              F  VE  +   ++      WVP      
Sbjct: 783  QW------------------------------FASVEGFRTATMDAINNVKWVP-HQAVN 842

Query: 736  RFHNWLENARDWAVSRSRFWGTPLPVWVS-EDGEEILVMDSIEKLEKLSGAKVFDLHRHK 795
            R      +  DW +SR R WG P+P +   +  E ++  ++I  ++ +   K  D   + 
Sbjct: 843  RISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYM 902

Query: 796  IDHITIPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEG 855
                 +P K   +     +  D  D WF+SGS  +A +    E +     +FP     EG
Sbjct: 903  SVEDLLPEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEG 907

Query: 856  LDQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVND--- 915
             DQ RGWF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  ++     
Sbjct: 963  TDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKN 907

Query: 916  ------YGADALRLYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLE 975
                  YGAD +RL+ ++S     + L   +  +   + D++       R+L+ N     
Sbjct: 1023 SKDAPAYGADVMRLW-VSSVDYTGDVLIGPQ--ILRQMSDIYRKLRGTLRYLLGNLHDWR 907

Query: 976  IEGLAQFSPLDQATLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKF-LDN 1035
            +         D A   +   ++DQ      +++V  +++  + Y+ + +   + +F + +
Sbjct: 1083 V---------DNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVD 907

Query: 1036 LTNIYVRFNRKRL-KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNL 1070
            L+N Y    + RL  G T     R   + L   LL+  +V+AP  P   E ++QNL
Sbjct: 1143 LSNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNL 907

BLAST of Cp4.1LG07g05300 vs. TAIR 10
Match: AT3G23145.1 (zinc ion binding;isoleucine-tRNA ligases;ATP binding;aminoacyl-tRNA ligases;nucleotide binding )

HSP 1 Score: 138.7 bits (348), Expect = 3.8e-32
Identity = 80/199 (40.20%), Postives = 94/199 (47.24%), Query Frame = 0

Query: 180 MPYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALC 239
           MPYSTGCKTPLSNFEAGQ+YK             ++D  N A VAWTTTPW LPSNLALC
Sbjct: 1   MPYSTGCKTPLSNFEAGQNYK-------------IQD--NTALVAWTTTPWILPSNLALC 60

Query: 240 VNANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSK 299
           VNA F+Y+K+                                                  
Sbjct: 61  VNAKFVYLKI-------------------------------------------------- 116

Query: 300 TENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCA 359
                               +G+        F +FS V FR+VAD YV +DSGTGIVHCA
Sbjct: 121 ------------------PWLGRSMNLCLITFSDFSSVTFRLVADDYVMNDSGTGIVHCA 116

Query: 360 PAFGEDDYRVCIENQVINK 379
           P FG DDYRVC+EN++I K
Sbjct: 181 PVFGADDYRVCLENEIIKK 116