Cp4.1LG07g04630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG07g04630 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.0e-104
Identity = 214/540 (39.63%), Postives = 320/540 (59.26%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           + GG  AA F+   E LEN+AF+A A +L+ Y   +M+ SL +S++ +T FM T FLL L
Sbjct: 27  RHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLAL 86

Query: 77  VGGF--------------------------------------PCKGAAAGSECKAANGGQ 136
           +GGF                                      PCK +AA   C+   G +
Sbjct: 87  LGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAA-LRCEVVGGSK 146

Query: 137 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 196
            A L+VG+YL++LG  G+K +LP+ GA+QF+EG P    + S+FFN+++F L+ G+ V +
Sbjct: 147 AAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAV 206

Query: 197 TLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIH 256
           T +VWI    GW+W F V TI++  +ILV   G  F++N +P+GSP+  I +V++ ASI 
Sbjct: 207 TFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIV 266

Query: 257 NRKLPLPDNADELHEI-CDKEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRLCTVTQV 316
           +       N     E+  + E   P + L  T+    L +A  I   T   W  CTV QV
Sbjct: 267 SCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKA--IEGKTHHIWLECTVQQV 326

Query: 317 EETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMF 376
           E+ KI+++MLPI   TI +  C+AQL T+S+ Q+ TM+   + F +P  S+PV P++FM 
Sbjct: 327 EDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFML 386

Query: 377 FFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNM 436
              P Y+ + +P ARK+T    GI HLQRIG GL+L+  +MA+A  VE +RK VA +  +
Sbjct: 387 ILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGL 446

Query: 437 VDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGY 496
           +DS+E LP++  W++ Q+   G AD+FT+ GLLEFF+ E+ + M+SL+T+++W S+A GY
Sbjct: 447 LDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGY 506

Query: 497 FTSTVVVEVVNKAS-----GGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRY 512
           + S+V+V +VN+ +       WL    LNR+ L+ FYWL+ VLSVVNF  YL  A  Y+Y
Sbjct: 507 YLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Cp4.1LG07g04630 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 3.6e-102
Identity = 214/556 (38.49%), Postives = 320/556 (57.55%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           + GG  AA F+ V E LEN+A++A A +L+ Y   YM+ S +KSA  +TNFMGT FLL L
Sbjct: 25  RHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLAL 84

Query: 77  VGGFPCKG----------------------------------AAAGSECKAANGGQEAIL 136
           +GGF                                      +     C+  +G + A+L
Sbjct: 85  LGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAML 144

Query: 137 YVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIV 196
           +VG+YL+ALG  G+K +L + GA+QF+E  P    + S+FFN+F+F L  G+ V +T +V
Sbjct: 145 FVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVV 204

Query: 197 WINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIHNRKL 256
           W+    GW+W F V TIA+  +IL+  +G  F+RN +P GSP+  IL+V++ AS+     
Sbjct: 205 WLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSS 264

Query: 257 PLPDNADELHEI-----CDKEADIPIEI---LEKTDQFRFLERAAIIRN---------DT 316
               NA     +     C  +    +E    LEK  Q   L   A + N         D 
Sbjct: 265 GSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADE 324

Query: 317 RTPWRL--CTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKI 376
           +   RL  CTV QVE+ KI+++MLPI   TI +  C+AQL TFS+QQ+ +M++     KI
Sbjct: 325 KPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKI 384

Query: 377 PGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF 436
           P  S+P+ P++F+    P Y+ + +P ARK T   TG+ HLQRIG GL+L+  +MA+A  
Sbjct: 385 PPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAAL 444

Query: 437 VETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKS 496
           VE +RK VA    ++DS+E LP++  W++ Q+   G AD+FT+ GLLE+F+ E+ + M+S
Sbjct: 445 VEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRS 504

Query: 497 LSTAIAWCSIAFGYFTSTVVVEVVNKASGG-----WLESNNLNRDNLNYFYWLLSVLSVV 514
           L+T+++W S+A GY+ S+V+V +VN  +G      WL   ++NR  L+YFYWL+ VLS  
Sbjct: 505 LATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAA 564

BLAST of Cp4.1LG07g04630 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.1e-94
Identity = 212/542 (39.11%), Postives = 308/542 (56.83%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           K GG RAA F+   +  E MA  A+  +L+TY    M+F L+KSA  +TNF+GT FLL+L
Sbjct: 38  KHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSL 97

Query: 77  VGGF--------------------------------------PCKGAAAGSECKAANGGQ 136
           +GGF                                       C   +    C  ANG +
Sbjct: 98  LGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYK 157

Query: 137 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 196
            A LY  + L+ALG+  +K  + + GA+QF+  D     KLSSFFN   F+ + G  + L
Sbjct: 158 AATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIAL 217

Query: 197 TLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHN 256
           TL+VW+ T  G D  F V    + A ++ + AG SF+RN  P GS    I QV VA+I  
Sbjct: 218 TLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITK 277

Query: 257 RKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRND--TRTPWRLCTVTQV 316
           RK   P N + +H+       + ++ L  +++FRFL++A I        +PWRLCT+ QV
Sbjct: 278 RKQICPSNPNMVHQ--PSTDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQV 337

Query: 317 EETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFL-GFKIPGPSVPVIPLLFM 376
            + KIL+ ++PI   TI   T +AQLQTFS+QQ  +M+++    F+IP  S+  IP + +
Sbjct: 338 HQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIIL 397

Query: 377 FFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHN 436
            FF+P YE  FVPLARK+TG  +GI  LQRIG GL L   SM  A  VE +R+   ++ N
Sbjct: 398 IFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQN 457

Query: 437 MVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFG 496
           ++       +S+FW++ QF +FG++++FT VGL+EFFY +SS  M+S  TA+ +CS +FG
Sbjct: 458 VM-------LSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFG 517

Query: 497 YFTSTVVVEVVNKASG--------GWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCAS 510
           ++ S+V+V  VN+ +         GWL  N+LN+D L++FYWLL+ LS +NF  YL  + 
Sbjct: 518 FYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSR 567

BLAST of Cp4.1LG07g04630 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 4.0e-93
Identity = 210/540 (38.89%), Postives = 303/540 (56.11%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           K GG RAA F+   +  E M   A+  +L+TY    M+F L+K+A  +TNF+GT F+  L
Sbjct: 41  KHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFAL 100

Query: 77  VGGF-------------------------------------PCKGAAAGSECKAANGGQE 136
           +GG+                                     P         C+ A G + 
Sbjct: 101 LGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKA 160

Query: 137 AILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLT 196
            I ++ +YL+ALG+  VK  + A GADQF +  P ++ +LSS+FN   F+ + G  + LT
Sbjct: 161 MIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALT 220

Query: 197 LIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHNR 256
           L+VW+ T  G D  F V   A+   I+ + +G  +FRN  P+ S    I  VIVA+I  R
Sbjct: 221 LLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKR 280

Query: 257 KLPLPDNADELHEICDKEAD-IPIEILEKTDQFRFLERAAIIRNDTRT---PWRLCTVTQ 316
           KL  P +   LH       D +P   L  T +FRFL++A I   DT T   PWRLCTVTQ
Sbjct: 281 KLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQ 340

Query: 317 VEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFL-GFKIPGPSVPVIPLLF 376
           VE+ K LI ++PI  STI   T +AQLQTFS+QQ  +M++     F IP  S+  IP + 
Sbjct: 341 VEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIM 400

Query: 377 MFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQH 436
           + F +P Y+   VP ARK+TG  +GI  L RIG GL L+  SM  A  +E +R++ ++  
Sbjct: 401 LIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLD 460

Query: 437 NMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAF 496
             +       +S+FW++ QF +FG++++FT VGL+EFFY +S+ GM+S   A+ +CS +F
Sbjct: 461 GRI-------LSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSF 520

Query: 497 GYFTSTVVVEVVNKA------SGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASW 509
           G++ S+V+V VVNK       S GWL  N+LN+D L+ FYWLL+VLS++NF  YL  + W
Sbjct: 521 GFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Cp4.1LG07g04630 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 338.6 bits (867), Expect = 1.3e-91
Identity = 203/532 (38.16%), Postives = 306/532 (57.52%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           + GG  AA F+ V E LEN+AF+A A +L+ Y +  M FS + +A  +T FMGT F L L
Sbjct: 56  RHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAANAVTAFMGTAFFLAL 115

Query: 77  VGGFPCKG---------AAAGSEC------------KAANGGQEAILYVGIYLIALGASG 136
           +GGF              +A  E              +        L+VG+YL+ALG  G
Sbjct: 116 LGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEPWSRVFLFVGLYLVALGVGG 175

Query: 137 VKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFV 196
           +K +LP  GA+QF+E   +   + S FFN+F+FSL+ G+ + +T++VW+    GW + F 
Sbjct: 176 IKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFG 235

Query: 197 VCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIH--NRKLPLPDNADELHEI 256
           V T A+L ++ V  AG   +R  VP GSPI  + +V+ A+++   +K          H  
Sbjct: 236 VSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTR 295

Query: 257 CDKEADIPIEILEKTDQF--RFLERAAIIRNDTRTPWRLCTVTQVEETKILIRMLPIIVS 316
            D +  +  +  +  D F   FL      R     P R CT  QV++ KI+I++LPI +S
Sbjct: 296 NDCDDSVTKQNCDGDDGFLGSFLGEVVRERESLPRPLR-CTEEQVKDVKIVIKILPIFMS 355

Query: 317 TIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPLAR 376
           TI +  C+AQL TFS+QQ+ TM++    F +P  ++PV P++FM    P Y  + +PLAR
Sbjct: 356 TIMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLAR 415

Query: 377 KITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAI-----QHNMVDSQEPLPMS 436
           K T   TGI HLQRIG GL+L+  +MA+A  VET+RK+V +      ++   S  PLP++
Sbjct: 416 KSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPIT 475

Query: 437 VFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVV 496
             W++ Q+   G AD+FT+ G++EFF+ E+ + M+SL+T+++W S+A GY+ S+V+V  V
Sbjct: 476 FLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAV 535

Query: 497 NKASG-----GWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKN 514
           N  +G      WL   NLN+ +L  FYWL+ VLS +NF  YL  AS Y Y++
Sbjct: 536 NFVTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRS 586

BLAST of Cp4.1LG07g04630 vs. NCBI nr
Match: XP_023537870.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1021 bits (2639), Expect = 0.0
Identity = 530/569 (93.15%), Postives = 531/569 (93.32%), Query Frame = 0

Query: 1   MGICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKS 60
           +GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKS
Sbjct: 15  LGICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKS 74

Query: 61  ATTLTNFMGTTFLLTLVGGF--------------------------------------PC 120
           ATTLTNFMGTTFLLTLVGGF                                      PC
Sbjct: 75  ATTLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVVFACFELLGYCLLTAQAHFRELRPKPC 134

Query: 121 KGAAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSF 180
           KGAAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSF
Sbjct: 135 KGAAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSF 194

Query: 181 FNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKG 240
           FNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKG
Sbjct: 195 FNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKG 254

Query: 241 SPILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRN 300
           SPILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRN
Sbjct: 255 SPILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRN 314

Query: 301 DTRTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKI 360
           DTRTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKI
Sbjct: 315 DTRTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKI 374

Query: 361 PGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF 420
           PGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF
Sbjct: 375 PGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF 434

Query: 421 VETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKS 480
           VETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKS
Sbjct: 435 VETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKS 494

Query: 481 LSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFY 531
           LSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFY
Sbjct: 495 LSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFY 554

BLAST of Cp4.1LG07g04630 vs. NCBI nr
Match: XP_023002887.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 518/566 (91.52%), Postives = 523/566 (92.40%), Query Frame = 0

Query: 2   GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSA 61
           GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYF+GYMNFSLTKSA
Sbjct: 15  GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFSGYMNFSLTKSA 74

Query: 62  TTLTNFMGTTFLLTLVGGF--------------------------------------PCK 121
           TT+TNFMGTTFLLTLVGGF                                      PCK
Sbjct: 75  TTMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYFLLSAQAHFRELRPEPCK 134

Query: 122 GAAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFF 181
           GA AGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFF
Sbjct: 135 GALAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFF 194

Query: 182 NWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGS 241
           NWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGS
Sbjct: 195 NWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGS 254

Query: 242 PILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRND 301
           PILRILQVIVASIHNRKLPLP+NADELHEI DKEADIPIEILEKTDQFRFLERAAIIRND
Sbjct: 255 PILRILQVIVASIHNRKLPLPENADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRND 314

Query: 302 TRTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIP 361
           TRT WRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIP
Sbjct: 315 TRTLWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIP 374

Query: 362 GPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFV 421
           GPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFV
Sbjct: 375 GPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFV 434

Query: 422 ETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSL 481
           ETRRKNVAIQHNMVDS+EPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSL
Sbjct: 435 ETRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSL 494

Query: 482 STAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYL 529
           STAIAWCSIAFGYFTSTVVVEVVNKASGGWL SNNLNRDNLNYFYWLLSVLSVVNFGFYL
Sbjct: 495 STAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYL 554

BLAST of Cp4.1LG07g04630 vs. NCBI nr
Match: KAG6585639.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 994 bits (2569), Expect = 0.0
Identity = 515/561 (91.80%), Postives = 521/561 (92.87%), Query Frame = 0

Query: 1   MGICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKS 60
           MG+CGLGAKNGDKKGPKRGGNRAAGFIYV EGLENMAFVALALSLMTYFNGYMNFSLTKS
Sbjct: 248 MGMCGLGAKNGDKKGPKRGGNRAAGFIYVIEGLENMAFVALALSLMTYFNGYMNFSLTKS 307

Query: 61  ATTLTNFMGTTFLLTLVGGF------------------------------PCKGAAAGSE 120
           ATTLTNFMGTTFLLTLVGGF                              PCKG AAGSE
Sbjct: 308 ATTLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLAHFRELRPEPCKGTAAGSE 367

Query: 121 CKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSL 180
           CKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSL
Sbjct: 368 CKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSL 427

Query: 181 TTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQ 240
           TTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVC+GKSFFRNNVPKGSPILRILQ
Sbjct: 428 TTGSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAILVVCSGKSFFRNNVPKGSPILRILQ 487

Query: 241 VIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRL 300
           VIVASI NRKLPLP+N DELHEI  KEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRL
Sbjct: 488 VIVASIRNRKLPLPNNVDELHEIRGKEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRL 547

Query: 301 CTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVI 360
           CTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMD NF+GFKIPGPSVPVI
Sbjct: 548 CTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDCNFIGFKIPGPSVPVI 607

Query: 361 PLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNV 420
           PLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNV
Sbjct: 608 PLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNV 667

Query: 421 AIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWC 480
           AIQHNMVDS+EPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWC
Sbjct: 668 AIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWC 727

Query: 481 SIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYR 531
           SIAFGYFTSTVVVEVVNKASGGWL SNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYR
Sbjct: 728 SIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYR 787

BLAST of Cp4.1LG07g04630 vs. NCBI nr
Match: XP_022951878.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 516/567 (91.01%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 62
           +CGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT
Sbjct: 1   MCGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60

Query: 63  TLTNFMGTTFLLTLVGGF--------------------------------------PCKG 122
           TLTNFMGTTFLLTLVGGF                                      PCKG
Sbjct: 61  TLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYCLLTAQAHFRELRPEPCKG 120

Query: 123 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 182
           AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 121 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180

Query: 183 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 242
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAILVVCAGKSFFRNNVPKGSP 240

Query: 243 ILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRNDT 302
           ILRILQVIVASI NRKLPLP+NADELHEI DKEADIPIEILEKTDQFRFLERAAIIRNDT
Sbjct: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRNDT 300

Query: 303 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPG 362
           RTPWRLCTVTQVEETKILIRMLPIIVSTIFMA+CMAQLQTFSIQQSLTMDSNF+GFKIPG
Sbjct: 301 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPG 360

Query: 363 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 422
           PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 361 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420

Query: 423 TRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLS 482
           TRRKNVAIQHNMVDS+EPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSA MKSLS
Sbjct: 421 TRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLS 480

Query: 483 TAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLV 531
           TAIAWCSIAFGYFTSTVVVEVVNKASGGWL SNNLNRDNLNYFYWLLSVLSVVNFGFYLV
Sbjct: 481 TAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLV 540

BLAST of Cp4.1LG07g04630 vs. NCBI nr
Match: XP_022144215.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia])

HSP 1 Score: 872 bits (2252), Expect = 2.66e-315
Identity = 455/570 (79.82%), Postives = 488/570 (85.61%), Query Frame = 0

Query: 3   ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 62
           ICGLG +NG+KK  KRGG+RAA F+ VTEGLENMAFV+LALSL+TYF+GYMNFSLTKSAT
Sbjct: 4   ICGLGNENGEKKEAKRGGSRAAVFVCVTEGLENMAFVSLALSLVTYFSGYMNFSLTKSAT 63

Query: 63  TLTNFMGTTFLLTLVGGF--------------------------------------PCKG 122
           TLTNFMGTTFLLTL+GGF                                      PCKG
Sbjct: 64  TLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIFASIEFMGYGLLTAQAHFRELRPAPCKG 123

Query: 123 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 182
           A A  EC+AA+GGQEAILY+GIYLIALG SGVK ALPALGADQF+ GDPAEAGKLSSFFN
Sbjct: 124 AVA-RECEAADGGQEAILYLGIYLIALGTSGVKSALPALGADQFDGGDPAEAGKLSSFFN 183

Query: 183 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 242
           WFLFSLTTGS VGLTLIVWINT+VGWDWAFVVC++AV+AAI+ VC+GK F+RNN PKGSP
Sbjct: 184 WFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVCSLAVVAAIVAVCSGKKFYRNNAPKGSP 243

Query: 243 ILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRNDT 302
           I RI QV VASI NRKLPLP NADELHEI DKEA +P EILEKTDQFRFL+RAAIIRN T
Sbjct: 244 IQRIFQVFVASIRNRKLPLPKNADELHEIRDKEAAMPYEILEKTDQFRFLDRAAIIRNYT 303

Query: 303 RT--------PWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSN 362
                     PWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQS+TMDSN
Sbjct: 304 SASMAAEQQGPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSITMDSN 363

Query: 363 FLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAAS 422
           FLGFKIPGPSVPVIPLLFM F IPFYERVFVPLARKITGIPTGIRHLQRIG GL+LTAAS
Sbjct: 364 FLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAAS 423

Query: 423 MAIAGFVETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAES 482
           MAIAGFVETRRKN+AI+H+MVDS+EPLPMSVFWLSFQFC+FGM D+FT+VGLLEFFYAES
Sbjct: 424 MAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFWLSFQFCIFGMGDMFTLVGLLEFFYAES 483

Query: 483 SAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSV 526
           SAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRD LNYFYWLLSVLSV
Sbjct: 484 SAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDKLNYFYWLLSVLSV 543

BLAST of Cp4.1LG07g04630 vs. ExPASy TrEMBL
Match: A0A6J1KKU7 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE=3 SV=1)

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 518/566 (91.52%), Postives = 523/566 (92.40%), Query Frame = 0

Query: 2   GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSA 61
           GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYF+GYMNFSLTKSA
Sbjct: 15  GICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFSGYMNFSLTKSA 74

Query: 62  TTLTNFMGTTFLLTLVGGF--------------------------------------PCK 121
           TT+TNFMGTTFLLTLVGGF                                      PCK
Sbjct: 75  TTMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYFLLSAQAHFRELRPEPCK 134

Query: 122 GAAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFF 181
           GA AGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFF
Sbjct: 135 GALAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFF 194

Query: 182 NWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGS 241
           NWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGS
Sbjct: 195 NWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGS 254

Query: 242 PILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRND 301
           PILRILQVIVASIHNRKLPLP+NADELHEI DKEADIPIEILEKTDQFRFLERAAIIRND
Sbjct: 255 PILRILQVIVASIHNRKLPLPENADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRND 314

Query: 302 TRTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIP 361
           TRT WRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIP
Sbjct: 315 TRTLWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIP 374

Query: 362 GPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFV 421
           GPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFV
Sbjct: 375 GPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFV 434

Query: 422 ETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSL 481
           ETRRKNVAIQHNMVDS+EPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSL
Sbjct: 435 ETRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSL 494

Query: 482 STAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYL 529
           STAIAWCSIAFGYFTSTVVVEVVNKASGGWL SNNLNRDNLNYFYWLLSVLSVVNFGFYL
Sbjct: 495 STAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYL 554

BLAST of Cp4.1LG07g04630 vs. ExPASy TrEMBL
Match: A0A6J1GIY5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 PE=3 SV=1)

HSP 1 Score: 991 bits (2561), Expect = 0.0
Identity = 516/567 (91.01%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 62
           +CGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT
Sbjct: 1   MCGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60

Query: 63  TLTNFMGTTFLLTLVGGF--------------------------------------PCKG 122
           TLTNFMGTTFLLTLVGGF                                      PCKG
Sbjct: 61  TLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYCLLTAQAHFRELRPEPCKG 120

Query: 123 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 182
           AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 121 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180

Query: 183 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 242
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAILVVCAGKSFFRNNVPKGSP 240

Query: 243 ILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRNDT 302
           ILRILQVIVASI NRKLPLP+NADELHEI DKEADIPIEILEKTDQFRFLERAAIIRNDT
Sbjct: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRNDT 300

Query: 303 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPG 362
           RTPWRLCTVTQVEETKILIRMLPIIVSTIFMA+CMAQLQTFSIQQSLTMDSNF+GFKIPG
Sbjct: 301 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPG 360

Query: 363 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 422
           PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 361 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420

Query: 423 TRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLS 482
           TRRKNVAIQHNMVDS+EPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSA MKSLS
Sbjct: 421 TRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLS 480

Query: 483 TAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLV 531
           TAIAWCSIAFGYFTSTVVVEVVNKASGGWL SNNLNRDNLNYFYWLLSVLSVVNFGFYLV
Sbjct: 481 TAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLV 540

BLAST of Cp4.1LG07g04630 vs. ExPASy TrEMBL
Match: A0A6J1CR11 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 872 bits (2252), Expect = 1.29e-315
Identity = 455/570 (79.82%), Postives = 488/570 (85.61%), Query Frame = 0

Query: 3   ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 62
           ICGLG +NG+KK  KRGG+RAA F+ VTEGLENMAFV+LALSL+TYF+GYMNFSLTKSAT
Sbjct: 4   ICGLGNENGEKKEAKRGGSRAAVFVCVTEGLENMAFVSLALSLVTYFSGYMNFSLTKSAT 63

Query: 63  TLTNFMGTTFLLTLVGGF--------------------------------------PCKG 122
           TLTNFMGTTFLLTL+GGF                                      PCKG
Sbjct: 64  TLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIFASIEFMGYGLLTAQAHFRELRPAPCKG 123

Query: 123 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 182
           A A  EC+AA+GGQEAILY+GIYLIALG SGVK ALPALGADQF+ GDPAEAGKLSSFFN
Sbjct: 124 AVA-RECEAADGGQEAILYLGIYLIALGTSGVKSALPALGADQFDGGDPAEAGKLSSFFN 183

Query: 183 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 242
           WFLFSLTTGS VGLTLIVWINT+VGWDWAFVVC++AV+AAI+ VC+GK F+RNN PKGSP
Sbjct: 184 WFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVCSLAVVAAIVAVCSGKKFYRNNAPKGSP 243

Query: 243 ILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRNDT 302
           I RI QV VASI NRKLPLP NADELHEI DKEA +P EILEKTDQFRFL+RAAIIRN T
Sbjct: 244 IQRIFQVFVASIRNRKLPLPKNADELHEIRDKEAAMPYEILEKTDQFRFLDRAAIIRNYT 303

Query: 303 RT--------PWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSN 362
                     PWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQS+TMDSN
Sbjct: 304 SASMAAEQQGPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSITMDSN 363

Query: 363 FLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAAS 422
           FLGFKIPGPSVPVIPLLFM F IPFYERVFVPLARKITGIPTGIRHLQRIG GL+LTAAS
Sbjct: 364 FLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAAS 423

Query: 423 MAIAGFVETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAES 482
           MAIAGFVETRRKN+AI+H+MVDS+EPLPMSVFWLSFQFC+FGM D+FT+VGLLEFFYAES
Sbjct: 424 MAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFWLSFQFCIFGMGDMFTLVGLLEFFYAES 483

Query: 483 SAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLSVLSV 526
           SAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRD LNYFYWLLSVLSV
Sbjct: 484 SAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDKLNYFYWLLSVLSV 543

BLAST of Cp4.1LG07g04630 vs. ExPASy TrEMBL
Match: A0A6J1CSN2 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 825 bits (2132), Expect = 6.63e-298
Identity = 431/537 (80.26%), Postives = 460/537 (85.66%), Query Frame = 0

Query: 36  MAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGF--------------- 95
           MAFV+LALSL+TYF+GYMNFSLTKSATTLTNFMGTTFLLTL+GGF               
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 96  -----------------------PCKGAAAGSECKAANGGQEAILYVGIYLIALGASGVK 155
                                  PCKGA A  EC+AA+GGQEAILY+GIYLIALG SGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 156 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 215
            ALPALGADQF+ GDPAEAGKLSSFFNWFLFSLTTGS VGLTLIVWINT+VGWDWAFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 216 TIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHNRKLPLPDNADELHEICDKE 275
           ++AV+AAI+ VC+GK F+RNN PKGSPI RI QV VASI NRKLPLP NADELHEI DKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 276 ADIPIEILEKTDQFRFLERAAIIRNDTRT--------PWRLCTVTQVEETKILIRMLPII 335
           A +P EILEKTDQFRFL+RAAIIRN T          PWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 336 VSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 395
           VSTIFMATCMAQLQTFSIQQS+TMDSNFLGFKIPGPSVPVIPLLFM F IPFYERVFVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 396 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSQEPLPMSVFW 455
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAGFVETRRKN+AI+H+MVDS+EPLPMSVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 456 LSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 515
           LSFQFC+FGM D+FT+VGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 516 SGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVLQKQGEEGLD 526
           SGGWLESNNLNRD LNYFYWLLSVLSV+NFGFYL+CASWYRYKNVE+LQ   EE  D
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKD 536

BLAST of Cp4.1LG07g04630 vs. ExPASy TrEMBL
Match: A0A0A0LLC0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1)

HSP 1 Score: 753 bits (1945), Expect = 2.65e-270
Identity = 385/459 (83.88%), Postives = 413/459 (89.98%), Query Frame = 0

Query: 81  PCKGAAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLS 140
           PCK A AGSEC+AA   QEA LY G+YLIALG SGVK ALPALGADQF++ DP+EAGK+S
Sbjct: 30  PCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPALGADQFDDKDPSEAGKVS 89

Query: 141 SFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVP 200
           SFFNWFLFSLT GS VGLTLIVWINTEVGWDWAFVVC+I+VLAAI VV  GKSF+R+NVP
Sbjct: 90  SFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVLAAIFVVFLGKSFYRHNVP 149

Query: 201 KGSPILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAII 260
           +GSPILR LQV VASI NRKLPLP NA+ELHEI DKEA IP EILEKTDQFRFL+RAAII
Sbjct: 150 QGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKEAAIPYEILEKTDQFRFLDRAAII 209

Query: 261 RNDT--------RTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLT 320
           RNDT        + PWRLCTVTQVEETKILIRMLPII+STIFMATCMAQLQTFSIQQS+T
Sbjct: 210 RNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQTFSIQQSIT 269

Query: 321 MDSNFLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLIL 380
           MDS FLGFKIPGPS+PVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIG GL+L
Sbjct: 270 MDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVL 329

Query: 381 TAASMAIAGFVETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFF 440
           TAASMAIAGFVETRRKNVAI+HNMVDS EPLP+SVFWL FQFCVFGM DIFT+VGLLEFF
Sbjct: 330 TAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFF 389

Query: 441 YAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLESNNLNRDNLNYFYWLLS 500
           YAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKASGGWL SNNLN+D L+YFYWLLS
Sbjct: 390 YAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLASNNLNKDKLDYFYWLLS 449

Query: 501 VLSVVNFGFYLVCASWYRYKNVEVLQKQG-EEGLDMVQE 530
           VLSV+NFGFYLVCASWYRYKNVE+ Q    EE +DM +E
Sbjct: 450 VLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMARE 488

BLAST of Cp4.1LG07g04630 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 7.3e-106
Identity = 214/540 (39.63%), Postives = 320/540 (59.26%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           + GG  AA F+   E LEN+AF+A A +L+ Y   +M+ SL +S++ +T FM T FLL L
Sbjct: 23  RHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLAL 82

Query: 77  VGGF--------------------------------------PCKGAAAGSECKAANGGQ 136
           +GGF                                      PCK +AA   C+   G +
Sbjct: 83  LGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAA-LRCEVVGGSK 142

Query: 137 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 196
            A L+VG+YL++LG  G+K +LP+ GA+QF+EG P    + S+FFN+++F L+ G+ V +
Sbjct: 143 AAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAV 202

Query: 197 TLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIH 256
           T +VWI    GW+W F V TI++  +ILV   G  F++N +P+GSP+  I +V++ ASI 
Sbjct: 203 TFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIV 262

Query: 257 NRKLPLPDNADELHEI-CDKEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRLCTVTQV 316
           +       N     E+  + E   P + L  T+    L +A  I   T   W  CTV QV
Sbjct: 263 SCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKA--IEGKTHHIWLECTVQQV 322

Query: 317 EETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMF 376
           E+ KI+++MLPI   TI +  C+AQL T+S+ Q+ TM+   + F +P  S+PV P++FM 
Sbjct: 323 EDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFML 382

Query: 377 FFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNM 436
              P Y+ + +P ARK+T    GI HLQRIG GL+L+  +MA+A  VE +RK VA +  +
Sbjct: 383 ILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGL 442

Query: 437 VDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGY 496
           +DS+E LP++  W++ Q+   G AD+FT+ GLLEFF+ E+ + M+SL+T+++W S+A GY
Sbjct: 443 LDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGY 502

Query: 497 FTSTVVVEVVNKAS-----GGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRY 512
           + S+V+V +VN+ +       WL    LNR+ L+ FYWL+ VLSVVNF  YL  A  Y+Y
Sbjct: 503 YLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of Cp4.1LG07g04630 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 7.3e-106
Identity = 214/540 (39.63%), Postives = 320/540 (59.26%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           + GG  AA F+   E LEN+AF+A A +L+ Y   +M+ SL +S++ +T FM T FLL L
Sbjct: 27  RHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLAL 86

Query: 77  VGGF--------------------------------------PCKGAAAGSECKAANGGQ 136
           +GGF                                      PCK +AA   C+   G +
Sbjct: 87  LGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAA-LRCEVVGGSK 146

Query: 137 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 196
            A L+VG+YL++LG  G+K +LP+ GA+QF+EG P    + S+FFN+++F L+ G+ V +
Sbjct: 147 AAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAV 206

Query: 197 TLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIH 256
           T +VWI    GW+W F V TI++  +ILV   G  F++N +P+GSP+  I +V++ ASI 
Sbjct: 207 TFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIV 266

Query: 257 NRKLPLPDNADELHEI-CDKEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRLCTVTQV 316
           +       N     E+  + E   P + L  T+    L +A  I   T   W  CTV QV
Sbjct: 267 SCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKA--IEGKTHHIWLECTVQQV 326

Query: 317 EETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMF 376
           E+ KI+++MLPI   TI +  C+AQL T+S+ Q+ TM+   + F +P  S+PV P++FM 
Sbjct: 327 EDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFML 386

Query: 377 FFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNM 436
              P Y+ + +P ARK+T    GI HLQRIG GL+L+  +MA+A  VE +RK VA +  +
Sbjct: 387 ILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGL 446

Query: 437 VDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGY 496
           +DS+E LP++  W++ Q+   G AD+FT+ GLLEFF+ E+ + M+SL+T+++W S+A GY
Sbjct: 447 LDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGY 506

Query: 497 FTSTVVVEVVNKAS-----GGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRY 512
           + S+V+V +VN+ +       WL    LNR+ L+ FYWL+ VLSVVNF  YL  A  Y+Y
Sbjct: 507 YLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Cp4.1LG07g04630 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 373.6 bits (958), Expect = 2.6e-103
Identity = 214/556 (38.49%), Postives = 320/556 (57.55%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           + GG  AA F+ V E LEN+A++A A +L+ Y   YM+ S +KSA  +TNFMGT FLL L
Sbjct: 25  RHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLAL 84

Query: 77  VGGFPCKG----------------------------------AAAGSECKAANGGQEAIL 136
           +GGF                                      +     C+  +G + A+L
Sbjct: 85  LGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAML 144

Query: 137 YVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIV 196
           +VG+YL+ALG  G+K +L + GA+QF+E  P    + S+FFN+F+F L  G+ V +T +V
Sbjct: 145 FVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVV 204

Query: 197 WINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIHNRKL 256
           W+    GW+W F V TIA+  +IL+  +G  F+RN +P GSP+  IL+V++ AS+     
Sbjct: 205 WLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSS 264

Query: 257 PLPDNADELHEI-----CDKEADIPIEI---LEKTDQFRFLERAAIIRN---------DT 316
               NA     +     C  +    +E    LEK  Q   L   A + N         D 
Sbjct: 265 GSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADE 324

Query: 317 RTPWRL--CTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKI 376
           +   RL  CTV QVE+ KI+++MLPI   TI +  C+AQL TFS+QQ+ +M++     KI
Sbjct: 325 KPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKI 384

Query: 377 PGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF 436
           P  S+P+ P++F+    P Y+ + +P ARK T   TG+ HLQRIG GL+L+  +MA+A  
Sbjct: 385 PPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAAL 444

Query: 437 VETRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKS 496
           VE +RK VA    ++DS+E LP++  W++ Q+   G AD+FT+ GLLE+F+ E+ + M+S
Sbjct: 445 VEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRS 504

Query: 497 LSTAIAWCSIAFGYFTSTVVVEVVNKASGG-----WLESNNLNRDNLNYFYWLLSVLSVV 514
           L+T+++W S+A GY+ S+V+V +VN  +G      WL   ++NR  L+YFYWL+ VLS  
Sbjct: 505 LATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAA 564

BLAST of Cp4.1LG07g04630 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 1.5e-95
Identity = 212/542 (39.11%), Postives = 308/542 (56.83%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           K GG RAA F+   +  E MA  A+  +L+TY    M+F L+KSA  +TNF+GT FLL+L
Sbjct: 38  KHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSL 97

Query: 77  VGGF--------------------------------------PCKGAAAGSECKAANGGQ 136
           +GGF                                       C   +    C  ANG +
Sbjct: 98  LGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYK 157

Query: 137 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 196
            A LY  + L+ALG+  +K  + + GA+QF+  D     KLSSFFN   F+ + G  + L
Sbjct: 158 AATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIAL 217

Query: 197 TLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHN 256
           TL+VW+ T  G D  F V    + A ++ + AG SF+RN  P GS    I QV VA+I  
Sbjct: 218 TLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITK 277

Query: 257 RKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRND--TRTPWRLCTVTQV 316
           RK   P N + +H+       + ++ L  +++FRFL++A I        +PWRLCT+ QV
Sbjct: 278 RKQICPSNPNMVHQ--PSTDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQV 337

Query: 317 EETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFL-GFKIPGPSVPVIPLLFM 376
            + KIL+ ++PI   TI   T +AQLQTFS+QQ  +M+++    F+IP  S+  IP + +
Sbjct: 338 HQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIIL 397

Query: 377 FFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHN 436
            FF+P YE  FVPLARK+TG  +GI  LQRIG GL L   SM  A  VE +R+   ++ N
Sbjct: 398 IFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQN 457

Query: 437 MVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFG 496
           ++       +S+FW++ QF +FG++++FT VGL+EFFY +SS  M+S  TA+ +CS +FG
Sbjct: 458 VM-------LSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFG 517

Query: 497 YFTSTVVVEVVNKASG--------GWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCAS 510
           ++ S+V+V  VN+ +         GWL  N+LN+D L++FYWLL+ LS +NF  YL  + 
Sbjct: 518 FYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSR 567

BLAST of Cp4.1LG07g04630 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.9e-94
Identity = 210/540 (38.89%), Postives = 303/540 (56.11%), Query Frame = 0

Query: 17  KRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 76
           K GG RAA F+   +  E M   A+  +L+TY    M+F L+K+A  +TNF+GT F+  L
Sbjct: 41  KHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFAL 100

Query: 77  VGGF-------------------------------------PCKGAAAGSECKAANGGQE 136
           +GG+                                     P         C+ A G + 
Sbjct: 101 LGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKA 160

Query: 137 AILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLT 196
            I ++ +YL+ALG+  VK  + A GADQF +  P ++ +LSS+FN   F+ + G  + LT
Sbjct: 161 MIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALT 220

Query: 197 LIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHNR 256
           L+VW+ T  G D  F V   A+   I+ + +G  +FRN  P+ S    I  VIVA+I  R
Sbjct: 221 LLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKR 280

Query: 257 KLPLPDNADELHEICDKEAD-IPIEILEKTDQFRFLERAAIIRNDTRT---PWRLCTVTQ 316
           KL  P +   LH       D +P   L  T +FRFL++A I   DT T   PWRLCTVTQ
Sbjct: 281 KLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQ 340

Query: 317 VEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFL-GFKIPGPSVPVIPLLF 376
           VE+ K LI ++PI  STI   T +AQLQTFS+QQ  +M++     F IP  S+  IP + 
Sbjct: 341 VEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIM 400

Query: 377 MFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQH 436
           + F +P Y+   VP ARK+TG  +GI  L RIG GL L+  SM  A  +E +R++ ++  
Sbjct: 401 LIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLD 460

Query: 437 NMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAF 496
             +       +S+FW++ QF +FG++++FT VGL+EFFY +S+ GM+S   A+ +CS +F
Sbjct: 461 GRI-------LSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSF 520

Query: 497 GYFTSTVVVEVVNKA------SGGWLESNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASW 509
           G++ S+V+V VVNK       S GWL  N+LN+D L+ FYWLL+VLS++NF  YL  + W
Sbjct: 521 GFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573