Cp4.1LG07g04560 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g04560
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionUroporphyrinogen-III synthase
LocationCp4.1LG07: 2950972 .. 2957372 (-)
RNA-Seq ExpressionCp4.1LG07g04560
SyntenyCp4.1LG07g04560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCGCATTATGATGGAAGAGAAACTAAGAACCAATGGAGAATTGACACGTGTCATTGAATGGATTAATTAATTAATTAATTAGTTGGGTCATGGGTCTTATTATGTTAACATCTGGCCTGCCCATCCTCAGTCTTTCATCTCCGATCAACACGTTTCCCCATGGCTCCAGTTTCTCTCTCTTTGCTCTCTCATTCGCCTTCAGTTCTTCTTCTTCCTTCTCCTAGTCTGCAACTCTCTCGCCCAATCTTACTTCCTCCTAGAACGCTCCCTTCTTGTTCCTCTACTTCAATTTCTTCTGTTTCGATTACGTCTTCGTCGATTTTGCCGCCGAAAGTTGTTGTTACTAGAGAGCGCGGCAAGAATGGGAAGCTCGTTAGTGCTCTGGTAATTTCTTGATTTTGTAATCTGAGGTTGTTTTGTTCGGATTGTTGATTTGTTTTATTCTCGTTGATTGCTCGAGTTTATTTTTGTTTTGTTCTTGTTCGCGAGTTAGATTTTCGTTCTACTGAGGCATTGTGTTCGTCTATTGAATTAATGTGGTGGTGCGATTAGTAAGTTTATGCGTTTGTGTAAATGTTTAAATGAATGCTGGTTGTTTGAGTGTTTTGATTCATTTTTGCCTTCTCAAGTTCTTTACCGATCGTGGAGATCTTTCCGAAATGGTTGATCATTTTGAGACTGACTGCTACTTTTACTCTTGCTGGAATATTGCTTTTAGGAGACAACAGACGCATACTTTAGTGGACGATTCTTGACATTGCGTACATTGAAACTGTAATGGTTTTTAAGCACCAATGTAGTTTTGATTTTCCAGAAAGTAAAATATCTTCTATCACCAGTCGTGAGAAAATGACGCCTCTAAAAGCAAATAGGCTGATCTCGTAAATAGCCACAATATGAGTTATATTTCAGTTCTTAGGTACACTCGGAGTCTCAGGACTGTATTGAACCATTCCAGAGCTGCGCACGGTTGTAAACTACTTGTTAATCTCGATTCCTTTTTTGATATAATAGCTTATTCGTAATAGCTGCTCTGCAATAGCAATCTTAGGAAGCATTTATATTAAAAATATTGGTGCTCTTTTCCAATATGCACGTTGTACTTGCATGGTGTTCGTGCTTTAATAGGTGGAATATTTNGTTCTCTTTTCCAATATGCACATTGTACTTGCATGGTGTTCGTGCTTTAATAGGTGGAATATTTGAATGGAATCCTGTTAATTGCTAAGACTCACATAGTTTTTCAGCCAGAGAACTGATCTTTTGTGTTAATCAAGAATAAGCAGTGTTTAGGATGTAGAATAGCCGTGATGATTTATCTTGAGCTTTTGTTCATTATCATTCAGACATCCACTCATGAATAAGTATTTCTGACATGGACGCATTGTTGCTTAAAGTAGAGTTGCTGATGTTATACCCTGCATTTTACGGCTTCTCTCCTCATTTCCCTTTTGTATCATTGATTTCATTGGCCTATTGTATTATAGACTTTTCGTGAAGTTAAAATAATGGGAAAGCAAAAGTGGTGGGCCTTGATGTTTTTGCGTGCATGAGAATGGTACAATTCAATCTCTATGTGAAATCAATAAACTGGAGCATATTGAAATTCCTGATGTTGATGACAGGCAAAACATGGAATAAACTGTTTGGAGCTTCCCCTCATTCAACACAGACAAGGACCTGACTTGAATAGGCTACAGTCTGTACTTAGTGGTAAGTCCTTCAAGATTGGGTCTCTCTTCTCGTACTCCTTCAAAGATCTCACTATTCTTGTTGAAAGGAATCGGTTCTCTCCTTCTCAAGCACGCACCCACATAATCTTGCAGGAGTGTTTCTGCTGGCATTTATTTAACAGTAGTATTTATTAGTAATTTCAATGTAGCACAGGATATTTTGAAGACTAGAAAATGGCACAAGCCTTTACAATCTTCTTCTGTTCTTCATTAACAGATTCTGTGTTTGACTGGATTGTTATAACATCCCCTGAAGCTGGCTTGGTGTTCCTAGAGGCATGGAAGTAAGGGTTAGAACTAACTCACTAGACTCTCACCAGTCTTGTATGTCACAGTGTCAAATGTCAATTTTTCTTATTGACAGGTGGTTAATATTCCTAGATCTTTGCAAGCTTTGAAGTTTCTCGAGGTTTTTAAAATATGTTTTGAAAAATTTTCCTATGTCATATACTGGCAATTGTGAAACTTGTTTTTTTTGGTAGAGGTGTTAGGACGAGAAATTCATACTCGTCATACCAGTTGATCTAGCTCTTTAGAATTTGCTTAGTTATGGACAGTTTATTTCAAGTTACAGATGATCCAGCCCATGGCAACACCATATTACCATTTGATAATATGAATTATTATACTAGTACCATCAGATAAGTTAAATTATTTTACACCATTGAGTGCATTGTTAAGGCCTTTATTTTCAACCTGTATGCCTGCATGGTACATGAACTTTTCATTCAATTTGGAATGCGTTGTTGGTCAAGTGAGTTAAAACATGTTTCTGAATTTTATTTTACTGGTCATCTGTTTCTTTCTGTTGAATTGTATTTTGCATTGTTGCTTCTATATAATCATGATCCACGGTGCTCTATGTTTACGAATGACATGTGTACCAACTAAAGATTGGTCGATACTTTCACATCTTATTTTTCCATTAGAGCTGCTTTAGGTCATCCTTAAGTGATACCTTCGCATTATTTTGTCCTTAGGGCCGCTGGAATGCCAAAAGTCAGAATAGGTGTTGTGGGAGCTGGTACAGCTAACATTTTTGAGGAAGTGTTGCAATCGTCAAAACAACAACTTGATGTGGCGTTTGCACCTTCAAAAGGTAAGACACATGTCAGGTCATGTCTCATGCCTGACACATGATATTCATTATTTGCAGATAGTATTTGTTTGCATGATGTTATTAGTCTTTTGCCTATATCATTTACAGACTGTCTTAATTCAAATCAGATGATGATAAGACCTTGAAAAAAACTGTTGTTGTTTGGAACATCAGAACTTTAAAACACAACGTGAAAAAATATGTCAAATGATTTAGATGGTACTTGTTCTAGTAGTATTTCATCTTCAATTATGTCTCTGTTATGATTGTTCTTCAACAATCACAATTTGGCAGGTATTTGTAATTTCACTATTTTTTTGGGTGGAGACTGTAATTTCACTATTACGTGTGTAGGCATCTGCATTTGATTTGGATTAGAATCCTTCTACAGAAATACATCGTTGTCCTACAAATATTAAAATTTGAATTTTATTTTCATCTATTATATCTTTCAGCAATAGGCAAGGTCTTGGCTTACGAGCTTCCAAAATTAGGGAATACGAAGTGCAGTGTCTTGTATCCTGCTTCCACTAAAGCAAGCAACGATATCGGTAGGCAATGTACTCTTTTTCTTCTACACTCTGAGGCTAGTTTCCATTAGATAATGTGGTAACAATGATGTTTCTTTTATTTATTTGTTTATTAAAATAGTTACTCTTGTTTCCTCATGCCATAACTATAAGGAATTTTATTTTGACTGGAAGTATTGTAATAAGTCCAATTTTACCTAGACCAGTAAACCTAATTTCCACAACTGAAATTTTCAAAATACTTCTAATCAGATGAAAAATTTATAATAGCATTCCCTACAAATGAGTTCGGACATCTCTATACCTTAAATTCACGCAAAATTGGTAGTGTGGATAACTGCCAAGTCTCTGTGTTCTATTCTAATCACTTCATAAGTAGCTTTTGGTCTGAAGAAATCCAAGGAAGAGGAAGTTATTTATAGAAGTACTTATAATGTGTGCGAGATAGTGTGCTTGAAACTAGATAGATGTGGGCTTGTATTGCTAAAATCATTTATTCTAGCTATAGCTCATATTTCAATGCAGCCTGATTTCTCTTCGTGTATGTATAGTATATGCATATTCGACTAACGTATAGTAGGGCTATGAGTAATTTGAACCTTGGTTGGAAATCTTAAATCAATTTTGAATCCCTGGTGAAAATTTTAAAATCAATTGCTATTGTAGTTTAGCTTAGTCATTATTAATGATAACAAGCCATTATTATTTACAAGATATACATTCCTCCCCATTGCTCCAAATTTTGTTTATCCTTTACTAACAGTTGGAAAACTGAACTGGCAAGCCTCAGTTTTATTCAACATTTATGTTTTCTTTTGTACACTTAATTTATGCGAACTGTAGTTGTGACTTGAAATTCTAATGTCAAGTGATCTGTTTGCAAGTATGAAGTGGCCGACTTGAACGAGTTCTTTTATCTGTTACTTTAAAAAGGAAAATTGAAGTTTTAGTACTTGTTCGTCTCATTGTAGCATTAGTATGCTGCTTTAGTATTGTTCTGTCTGTTCTAACTGAAAGCAAAATAATGGCTTTGAGAAATATAACCTTGGTGTCCCCTTTTGCAATCTTCAGAGGAGGGTCTTGCAAATCGTGGATTTGAGGTCACGAGGCTTAATACATACACTACGGTAAAGTTATCTTGTACACCACAAATTTAAAAACATGCCACACTGATTTTTAGAAGCGTATTCATCTACTTGATCATAAGTTCTTGATAAATATCTTGTATGCTTCAAATTTAGACCGAACCAAATTTAGACCCTCGTTGAAGAGGTTCGTTGGACTATACTTGAATTTTTATCAATTCTGTAGTACACATTTCCAAACCCGTGGGAGTCTTAAATCGTAACACCTTGTTCGTTCCTGTACTCTCAGTAACCTGAATGATGACTATGAAAAGAATATATGTTTCAAACGCAATTATCTTCGTAGGCATTATAATTATTCCTGGTATCTTTCAGGCACCCGTTGATTATGTCGATCAATCCATTCTCGAGCAAGCACTTCTGTTGCCTGTTGTCGCAGTGGCTTCACCTTCTGCAGTTCGGTAAGCACCAGTTTCTTTCTGATTAAAATTTGTTCTTTCCCGAGGGCAAGAGAATTTGTAATATTAATTAGTTCATTCCATCTTCATGGATCACAAATCTGTTGCAACAGCGCATGGATCAATCTTATTTCAGAGCCAAAGGAGTGGGAAAATTCACTTGCCTGTATTGGGGAAACAACTGCAGCAGTAGCGAAAAGATTAGGATTGAAAAACGTTTACTACCCGAAGAATCCTTGCCTTGAAGGGTGAGTTTTTCCATAATTCTACCTTACAATCTAGAAGTTAGTTCCTGGTAACCACCCACCTATAATTTTTTTAGCAACCATATTTGGTTGGGTCGACTATTGTACTATTAGATTAGTCATGCACTCACGGATATAATAATAAGAATAACAATAATAATAGTAAATCTTTAGTTGATGCATTTATTTTCATAATTGAGATTCCATGCTTTACAATAAGATCAAATAGACAAAATGTGAATTTACAAACGTGATGATTGAAATGCAGGTGGGTTGATAGTATATTGGAAGCAGTAAGAGCAGAAGAAGCACAAGTCGTTTAGGAAGTCATCTTTACTTCTTTTCCAGAGTTGCAGACGGAGACAAGAATTGCTTAAAGTTGTGGAACAAAGCATTCAACCATTGTATCATCATATGGTATGATCACAGTCGATTTGCCCGAGTTTCTATTCGAGGTTAGTCGCTTTTCCAGTAGTTTTACGTTCATTTATCTGTCTGTCCAGTTCGATATCAGATAATTAAATGACTCATTGTCATATTCTCTCTGTTGTTAATAGAATGTAAATTCGTTGAATAGGATAGGTTTGTCGTCTATTATCCAAATCTTCTTTCTTTTCTCTTCTTAATTTCCAACTTTGGTTTGAAATTTTTAGTAAACTCCAACTATAGTTTGTTAATTAATTGAGAGATTCCACCAGTAGTCATTCCTTAGCATTTATATATTAAAAAATTTAATGTTATCAACAAAGAATAAATCCATGGTTTAGCAGATCAAATACACGAGTTCAGGATTATAAACTTGTGTGAGTTATTTTATTTTTAAAATTTGAGATCATACGTAGCTTAATCTTTATGCTATTAATATTTTGTTCAACACTTTTGAGTATTGTTTAATCTGGAATCTTTATCCTTGAATTTATATAAACATCGTCTACTCTTGATGCTTTAAATTGTTGCCGCAGCCTATTATTCAACAGCAGGAAAATAAAAATTGCACCCGGATTCTAAACAAAATATGTTAAATATTGAGGTAAATAGGTGAAGGAAAATTAATTTGTATCCATTTTCTCTTTTAAAATAGTTTTTATTAAATTTATCTCTAACGTTGTGCATGTATTGCAAATTTTATCTTATGTTCAATTTTGTTCTAAATTTAATTCAATTTTTAATAAGATGTCAAGTAAACTTTGCTTAAAACATACTAATCATAAAAGAAACGTACAAAATTAACTGTAATTCATCTATAGTG

mRNA sequence

CAGCGCATTATGATGGAAGAGAAACTAAGAACCAATGGAGAATTGACACGTGTCATTGAATGGATTAATTAATTAATTAATTAGTTGGGTCATGGGTCTTATTATGTTAACATCTGGCCTGCCCATCCTCAGTCTTTCATCTCCGATCAACACGTTTCCCCATGGCTCCAGTTTCTCTCTCTTTGCTCTCTCATTCGCCTTCAGTTCTTCTTCTTCCTTCTCCTAGTCTGCAACTCTCTCGCCCAATCTTACTTCCTCCTAGAACGCTCCCTTCTTGTTCCTCTACTTCAATTTCTTCTGTTTCGATTACGTCTTCGTCGATTTTGCCGCCGAAAGTTGTTGTTACTAGAGAGCGCGGCAAGAATGGGAAGCTCGTTAGTGCTCTGGCAAAACATGGAATAAACTGTTTGGAGCTTCCCCTCATTCAACACAGACAAGGACCTGACTTGAATAGGCTACAGTCTGTACTTAGTGATTCTGTGTTTGACTGGATTGTTATAACATCCCCTGAAGCTGGCTTGGTGTTCCTAGAGGCATGGAAGGCCGCTGGAATGCCAAAAGTCAGAATAGGTGTTGTGGGAGCTGGTACAGCTAACATTTTTGAGGAAGTGTTGCAATCGTCAAAACAACAACTTGATGTGGCGTTTGCACCTTCAAAAGCAATAGGCAAGGTCTTGGCTTACGAGCTTCCAAAATTAGGGAATACGAAGTGCAGTGTCTTGTATCCTGCTTCCACTAAAGCAAGCAACGATATCGAGGAGGGTCTTGCAAATCGTGGATTTGAGGTCACGAGGCTTAATACATACACTACGGCACCCGTTGATTATGTCGATCAATCCATTCTCGAGCAAGCACTTCTGTTGCCTGTTGTCGCAGTGGCTTCACCTTCTGCAGTTCGCGCATGGATCAATCTTATTTCAGAGCCAAAGGAGTGGGAAAATTCACTTGCCTGTATTGGGGAAACAACTGCAGCAGTAGCGAAAAGATTAGGATTGAAAAACGTTTACTACCCGAAGAATCCTTGCCTTGAAGGGTGGGTTGATAGTATATTGGAAGCAGTAAGAGCAGAAGAAGCACAAGTCGTTTAGGAAGTCATCTTTACTTCTTTTCCAGAGTTGCAGACGGAGACAAGAATTGCTTAAAGTTGTGGAACAAAGCATTCAACCATTGTATCATCATATGGTATGATCACAGTCGATTTGCCCGAGTTTCTATTCGAGGTTAGTCGCTTTTCCAGTAGTTTTACGTTCATTTATCTGTCTGTCCAGTTCGATATCAGATAATTAAATGACTCATTGTCATATTCTCTCTGTTGTTAATAGAATGTAAATTCGTTGAATAGGATAGGTTTGTCGTCTATTATCCAAATCTTCTTTCTTTTCTCTTCTTAATTTCCAACTTTGGTTTGAAATTTTTAGTAAACTCCAACTATAGTTTGTTAATTAATTGAGAGATTCCACCAGTAGTCATTCCTTAGCATTTATATATTAAAAAATTTAATGTTATCAACAAAGAATAAATCCATGGTTTAGCAGATCAAATACACGAGTTCAGGATTATAAACTTGTGTGAGTTATTTTATTTTTAAAATTTGAGATCATACGTAGCTTAATCTTTATGCTATTAATATTTTGTTCAACACTTTTGAGTATTGTTTAATCTGGAATCTTTATCCTTGAATTTATATAAACATCGTCTACTCTTGATGCTTTAAATTGTTGCCGCAGCCTATTATTCAACAGCAGGAAAATAAAAATTGCACCCGGATTCTAAACAAAATATGTTAAATATTGAGGTAAATAGGTGAAGGAAAATTAATTTGTATCCATTTTCTCTTTTAAAATAGTTTTTATTAAATTTATCTCTAACGTTGTGCATGTATTGCAAATTTTATCTTATGTTCAATTTTGTTCTAAATTTAATTCAATTTTTAATAAGATGTCAAGTAAACTTTGCTTAAAACATACTAATCATAAAAGAAACGTACAAAATTAACTGTAATTCATCTATAGTG

Coding sequence (CDS)

ATGGCTCCAGTTTCTCTCTCTTTGCTCTCTCATTCGCCTTCAGTTCTTCTTCTTCCTTCTCCTAGTCTGCAACTCTCTCGCCCAATCTTACTTCCTCCTAGAACGCTCCCTTCTTGTTCCTCTACTTCAATTTCTTCTGTTTCGATTACGTCTTCGTCGATTTTGCCGCCGAAAGTTGTTGTTACTAGAGAGCGCGGCAAGAATGGGAAGCTCGTTAGTGCTCTGGCAAAACATGGAATAAACTGTTTGGAGCTTCCCCTCATTCAACACAGACAAGGACCTGACTTGAATAGGCTACAGTCTGTACTTAGTGATTCTGTGTTTGACTGGATTGTTATAACATCCCCTGAAGCTGGCTTGGTGTTCCTAGAGGCATGGAAGGCCGCTGGAATGCCAAAAGTCAGAATAGGTGTTGTGGGAGCTGGTACAGCTAACATTTTTGAGGAAGTGTTGCAATCGTCAAAACAACAACTTGATGTGGCGTTTGCACCTTCAAAAGCAATAGGCAAGGTCTTGGCTTACGAGCTTCCAAAATTAGGGAATACGAAGTGCAGTGTCTTGTATCCTGCTTCCACTAAAGCAAGCAACGATATCGAGGAGGGTCTTGCAAATCGTGGATTTGAGGTCACGAGGCTTAATACATACACTACGGCACCCGTTGATTATGTCGATCAATCCATTCTCGAGCAAGCACTTCTGTTGCCTGTTGTCGCAGTGGCTTCACCTTCTGCAGTTCGCGCATGGATCAATCTTATTTCAGAGCCAAAGGAGTGGGAAAATTCACTTGCCTGTATTGGGGAAACAACTGCAGCAGTAGCGAAAAGATTAGGATTGAAAAACGTTTACTACCCGAAGAATCCTTGCCTTGAAGGGTGGGTTGATAGTATATTGGAAGCAGTAAGAGCAGAAGAAGCACAAGTCGTTTAG

Protein sequence

MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAVRAEEAQVV
Homology
BLAST of Cp4.1LG07g04560 vs. ExPASy Swiss-Prot
Match: O48721 (Uroporphyrinogen-III synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UROS PE=2 SV=2)

HSP 1 Score: 340.5 bits (872), Expect = 2.0e-92
Identity = 183/310 (59.03%), Postives = 227/310 (73.23%), Query Frame = 0

Query: 4   VSLSLLSHSPSVLLLP----------SPSLQLSRPILLPPRTLPSCSSTSISSVSIT-SS 63
           ++L LLSH   +   P          S    LS+P+   P  + +  S+S+SS S + SS
Sbjct: 1   MALLLLSHCSILSFQPPLSSSSSFHSSHIQSLSKPVFASPSPIRNSISSSVSSSSSSVSS 60

Query: 64  SILPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIV 123
           S   P+VVVTRERGKN +++ AL K+GI+ LELPLIQH +GPD +RL SVL+D  FDWI+
Sbjct: 61  SNSIPQVVVTRERGKNNQIIKALEKNGISSLELPLIQHARGPDFDRLASVLNDKSFDWII 120

Query: 124 ITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVL 183
           ITSPEAG VFLEAWK A  P+V+IGVVGAGTA +FEE ++S+   L VAF PSKA GKVL
Sbjct: 121 ITSPEAGSVFLEAWKTASSPEVQIGVVGAGTARVFEEAMKSADGLLHVAFTPSKATGKVL 180

Query: 184 AYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQAL 243
           A ELP+    + SVLYPAS KA NDI EGL+ RGFEV RLNTYTT PV  VD  +L+QAL
Sbjct: 181 ASELPEKVGKRSSVLYPASLKAGNDIVEGLSKRGFEVVRLNTYTTVPVQSVDTVLLQQAL 240

Query: 244 LLPVVAVASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGW 303
             PV++VASPSAVRAW++LI   ++W N +ACIGETTA+ A+RLGLKNVYYP+ P LEGW
Sbjct: 241 SAPVLSVASPSAVRAWLHLIQNEEQWSNYVACIGETTASAARRLGLKNVYYPEKPGLEGW 300

BLAST of Cp4.1LG07g04560 vs. ExPASy Swiss-Prot
Match: Q10QR9 (Uroporphyrinogen-III synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=UROS PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.2e-90
Identity = 170/282 (60.28%), Postives = 203/282 (71.99%), Query Frame = 0

Query: 28  PILLPPRTLPSC-------SSTSISSVSITSSSILPPKVVVTRERGKNGKLVSALAKHGI 87
           P   PP T P              +   I  SS  PP VVVTRERGKN KL++AL KH +
Sbjct: 11  PFSRPPATFPRARHAGGGRGRAGATGRFIACSSPPPPDVVVTRERGKNAKLIAALEKHNV 70

Query: 88  NCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGLVFLEAWKAAGMPKVRIGVVG 147
             LELPLI+H +GPD +RL +VL D  FDWI ITSPEA  VFLE WKAAG PKVRI VVG
Sbjct: 71  QSLELPLIKHVEGPDTDRLSAVLRDEKFDWITITSPEAAAVFLEGWKAAGNPKVRIAVVG 130

Query: 148 AGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLGNTKCSVLYPASTKASNDIEE 207
           AGT  +F+EV+Q +   L+VAF+PSKA+GK LA ELP+   T C VLYPAS KA ++I+ 
Sbjct: 131 AGTERVFDEVIQYNDGSLEVAFSPSKAMGKFLASELPRTTETTCKVLYPASAKAGHEIQN 190

Query: 208 GLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVASPSAVRAWINLISEPKEWEN 267
           GL+NRGFEVTRLNTYTT  V  VD  IL+ AL  PVVAVASPSA+RAW+NL S+   W N
Sbjct: 191 GLSNRGFEVTRLNTYTTVSVQDVDPLILKPALSAPVVAVASPSALRAWLNLASQVDNWGN 250

Query: 268 SLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAVRA 303
           ++ACIGETTA+ AK+ GLK++YYP  P L+GWV+SILEA+RA
Sbjct: 251 AIACIGETTASAAKKFGLKSIYYPTTPGLDGWVESILEALRA 292

BLAST of Cp4.1LG07g04560 vs. NCBI nr
Match: XP_023537361.1 (uroporphyrinogen-III synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590 bits (1522), Expect = 2.61e-212
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240

Query: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300
           SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV
Sbjct: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300

Query: 301 RAEEAQVV 308
           RAEEAQVV
Sbjct: 301 RAEEAQVV 308

BLAST of Cp4.1LG07g04560 vs. NCBI nr
Match: KAG6585644.1 (Uroporphyrinogen-III synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 575 bits (1481), Expect = 4.46e-206
Identity = 304/308 (98.70%), Postives = 304/308 (98.70%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPR LPSCSSTSISSVSITSSSI PPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRMLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240

Query: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300
           SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP LEGWVDSILEAV
Sbjct: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGWVDSILEAV 300

Query: 301 RAEEAQVV 308
           RAE AQVV
Sbjct: 301 RAE-AQVV 307

BLAST of Cp4.1LG07g04560 vs. NCBI nr
Match: XP_022951382.1 (uroporphyrinogen-III synthase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 572 bits (1475), Expect = 3.66e-205
Identity = 303/308 (98.38%), Postives = 303/308 (98.38%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLS PILLPPRTLPSCSSTSISSVSITSSSI PPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSCPILLPPRTLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300
           SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP LEGWVDSILEAV
Sbjct: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGWVDSILEAV 300

Query: 301 RAEEAQVV 308
           RAE AQVV
Sbjct: 301 RAE-AQVV 307

BLAST of Cp4.1LG07g04560 vs. NCBI nr
Match: XP_023002006.1 (uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 561 bits (1446), Expect = 9.28e-201
Identity = 298/308 (96.75%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSST ISS SITSSSI PPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTLISSASITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHG+NCLELPLIQHRQGPDLNRLQSVL DSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGVNCLELPLIQHRQGPDLNRLQSVLCDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQ LDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQ-LDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300
           SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP L+GWVDSILEAV
Sbjct: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLKGWVDSILEAV 300

Query: 301 RAEEAQVV 308
           RAE AQVV
Sbjct: 301 RAE-AQVV 306

BLAST of Cp4.1LG07g04560 vs. NCBI nr
Match: KGN62543.1 (hypothetical protein Csa_021895 [Cucumis sativus])

HSP 1 Score: 484 bits (1246), Expect = 3.26e-170
Identity = 265/309 (85.76%), Postives = 278/309 (89.97%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSS-ILPPKV 60
           MA VSLS  SH PSV++ PSP LQ    ILLP RTL   SSTS+SS SITSSS I  PKV
Sbjct: 1   MASVSLSSFSHPPSVVV-PSPCLQFYGRILLPRRTLACSSSTSVSSSSITSSSSISAPKV 60

Query: 61  VVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAG 120
           VVTRERGKNGKLV+ALA+HGINCLELPLIQHRQGPDLNRL SVLSDSVFDWIVITSPEAG
Sbjct: 61  VVTRERGKNGKLVNALARHGINCLELPLIQHRQGPDLNRLHSVLSDSVFDWIVITSPEAG 120

Query: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKL 180
           LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK  L+VAFAPSKAIGKVLA ELPKL
Sbjct: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKL-LEVAFAPSKAIGKVLASELPKL 180

Query: 181 GNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAV 240
           GNTKCSVLYPASTKASNDIEE L+NRGFEVTRLNTYTTAPVDYVDQ+ILEQA  LPVV V
Sbjct: 181 GNTKCSVLYPASTKASNDIEECLSNRGFEVTRLNTYTTAPVDYVDQTILEQARSLPVVTV 240

Query: 241 ASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEA 300
           ASPSAVRAW+NLIS+PKEW+ SLACIGETTAA A RLGLKNVYYPKNP LEGWVDSILEA
Sbjct: 241 ASPSAVRAWVNLISDPKEWDKSLACIGETTAAAANRLGLKNVYYPKNPGLEGWVDSILEA 300

Query: 301 VRAEEAQVV 308
           +R+E AQVV
Sbjct: 301 LRSE-AQVV 306

BLAST of Cp4.1LG07g04560 vs. ExPASy TrEMBL
Match: A0A6J1GHG9 (Uroporphyrinogen-III synthase OS=Cucurbita moschata OX=3662 GN=LOC111454223 PE=3 SV=1)

HSP 1 Score: 572 bits (1475), Expect = 1.77e-205
Identity = 303/308 (98.38%), Postives = 303/308 (98.38%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLS PILLPPRTLPSCSSTSISSVSITSSSI PPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSCPILLPPRTLPSCSSTSISSVSITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300
           SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP LEGWVDSILEAV
Sbjct: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLEGWVDSILEAV 300

Query: 301 RAEEAQVV 308
           RAE AQVV
Sbjct: 301 RAE-AQVV 307

BLAST of Cp4.1LG07g04560 vs. ExPASy TrEMBL
Match: A0A6J1KK69 (Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111496019 PE=3 SV=1)

HSP 1 Score: 561 bits (1446), Expect = 4.49e-201
Identity = 298/308 (96.75%), Postives = 300/308 (97.40%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKVV 60
           MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSST ISS SITSSSI PPKVV
Sbjct: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTLISSASITSSSISPPKVV 60

Query: 61  VTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAGL 120
           VTRERGKNGKLVSALAKHG+NCLELPLIQHRQGPDLNRLQSVL DSVFDWIVITSPEAGL
Sbjct: 61  VTRERGKNGKLVSALAKHGVNCLELPLIQHRQGPDLNRLQSVLCDSVFDWIVITSPEAGL 120

Query: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKLG 180
           VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQ LDVAFAPSKAIGKVLAYELPKLG
Sbjct: 121 VFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQ-LDVAFAPSKAIGKVLAYELPKLG 180

Query: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAVA 240
           NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV VA
Sbjct: 181 NTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVTVA 240

Query: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEAV 300
           SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNP L+GWVDSILEAV
Sbjct: 241 SPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPGLKGWVDSILEAV 300

Query: 301 RAEEAQVV 308
           RAE AQVV
Sbjct: 301 RAE-AQVV 306

BLAST of Cp4.1LG07g04560 vs. ExPASy TrEMBL
Match: A0A0A0LLB2 (Uroporphyrinogen-III synthase OS=Cucumis sativus OX=3659 GN=Csa_2G360600 PE=3 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 1.58e-170
Identity = 265/309 (85.76%), Postives = 278/309 (89.97%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSS-ILPPKV 60
           MA VSLS  SH PSV++ PSP LQ    ILLP RTL   SSTS+SS SITSSS I  PKV
Sbjct: 1   MASVSLSSFSHPPSVVV-PSPCLQFYGRILLPRRTLACSSSTSVSSSSITSSSSISAPKV 60

Query: 61  VVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAG 120
           VVTRERGKNGKLV+ALA+HGINCLELPLIQHRQGPDLNRL SVLSDSVFDWIVITSPEAG
Sbjct: 61  VVTRERGKNGKLVNALARHGINCLELPLIQHRQGPDLNRLHSVLSDSVFDWIVITSPEAG 120

Query: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKL 180
           LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSK  L+VAFAPSKAIGKVLA ELPKL
Sbjct: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKL-LEVAFAPSKAIGKVLASELPKL 180

Query: 181 GNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAV 240
           GNTKCSVLYPASTKASNDIEE L+NRGFEVTRLNTYTTAPVDYVDQ+ILEQA  LPVV V
Sbjct: 181 GNTKCSVLYPASTKASNDIEECLSNRGFEVTRLNTYTTAPVDYVDQTILEQARSLPVVTV 240

Query: 241 ASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEA 300
           ASPSAVRAW+NLIS+PKEW+ SLACIGETTAA A RLGLKNVYYPKNP LEGWVDSILEA
Sbjct: 241 ASPSAVRAWVNLISDPKEWDKSLACIGETTAAAANRLGLKNVYYPKNPGLEGWVDSILEA 300

Query: 301 VRAEEAQVV 308
           +R+E AQVV
Sbjct: 301 LRSE-AQVV 306

BLAST of Cp4.1LG07g04560 vs. ExPASy TrEMBL
Match: A0A6J1KA58 (Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111492039 PE=3 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 2.19e-170
Identity = 262/309 (84.79%), Postives = 276/309 (89.32%), Query Frame = 0

Query: 1   MAPVSLSLLSHSPSVLLLPSPSL-QLSRPILLPPRTLPSCSSTSISSVSITSSSILPPKV 60
           MAPVSLS  SH+PSV+  PSP L QL R ILLP RTL   SST  SS SITSSSI  PKV
Sbjct: 16  MAPVSLSSFSHTPSVV--PSPCLLQLYRRILLPRRTLACFSSTLTSSASITSSSISAPKV 75

Query: 61  VVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPEAG 120
           VVTRERGKNGKL+ ALA+HGINCLELPLIQH QGPDLNRL SVLSDSVFDWI+ITSPEAG
Sbjct: 76  VVTRERGKNGKLIDALARHGINCLELPLIQHSQGPDLNRLHSVLSDSVFDWIIITSPEAG 135

Query: 121 LVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELPKL 180
           LVFL+AWKAAGMPKVRIGVVGAGTANIF+EVLQSS   L+VAFAPSKAIGKVLA ELPKL
Sbjct: 136 LVFLQAWKAAGMPKVRIGVVGAGTANIFDEVLQSSNL-LEVAFAPSKAIGKVLASELPKL 195

Query: 181 GNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVVAV 240
           GNTKCSVLYPASTKASNDIEEGL+NRGFEV RLNTYTTAPVDYVDQ ILEQA  LPVV V
Sbjct: 196 GNTKCSVLYPASTKASNDIEEGLSNRGFEVMRLNTYTTAPVDYVDQFILEQARSLPVVTV 255

Query: 241 ASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSILEA 300
           ASPSA+RAW+NLISEPKEWE SLACIGETTAA AKRLGLKN+YYPKNP LEGWVDSILEA
Sbjct: 256 ASPSAIRAWVNLISEPKEWEKSLACIGETTAAAAKRLGLKNIYYPKNPGLEGWVDSILEA 315

Query: 301 VRAEEAQVV 308
           +R+E AQVV
Sbjct: 316 LRSE-AQVV 320

BLAST of Cp4.1LG07g04560 vs. ExPASy TrEMBL
Match: A0A6J1CQT7 (Uroporphyrinogen-III synthase OS=Momordica charantia OX=3673 GN=LOC111013898 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 1.53e-169
Identity = 262/311 (84.24%), Postives = 276/311 (88.75%), Query Frame = 0

Query: 1   MAPVSLSLLS---HSPSVLLLPSPSLQLSRPILLPPRTLPSCSSTSISSVSITSSSILPP 60
           MAPVSLS LS   H PSV++ PSP LQL R ILLP RT PSCSS++  S+  TSSSI  P
Sbjct: 1   MAPVSLSSLSSLSHPPSVVV-PSPCLQLCRRILLPRRT-PSCSSSA--SIPTTSSSISAP 60

Query: 61  KVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIVITSPE 120
           KVVVTRERGKNGKLV+ALAKHGINCLELPLIQHRQGPDLNRL SVL DSVFDWIVITSPE
Sbjct: 61  KVVVTRERGKNGKLVNALAKHGINCLELPLIQHRQGPDLNRLHSVLGDSVFDWIVITSPE 120

Query: 121 AGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVLAYELP 180
           AGLVFLEAWKAAGMPKVRIGVVGAGTANIFE+VLQSS Q LDVAFAPSKAIGKVLA ELP
Sbjct: 121 AGLVFLEAWKAAGMPKVRIGVVGAGTANIFEDVLQSSNQLLDVAFAPSKAIGKVLASELP 180

Query: 181 KLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQALLLPVV 240
           KLG+TKC VLYPASTKASNDIEE L+NRGFEV RLNTYTTAPVDYVDQ++LEQA  LPVV
Sbjct: 181 KLGDTKCRVLYPASTKASNDIEEALSNRGFEVMRLNTYTTAPVDYVDQTVLEQARSLPVV 240

Query: 241 AVASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGWVDSIL 300
            VASPSAVRAW+NLISEP EWE SLACIGETTAA A+R GLKNVYYPKNP LEGWVDSIL
Sbjct: 241 TVASPSAVRAWVNLISEPNEWEKSLACIGETTAAAAERFGLKNVYYPKNPGLEGWVDSIL 300

Query: 301 EAVRAEEAQVV 308
           EA+R E AQVV
Sbjct: 301 EALRTE-AQVV 306

BLAST of Cp4.1LG07g04560 vs. TAIR 10
Match: AT2G26540.1 (uroporphyrinogen-III synthase family protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.4e-93
Identity = 183/310 (59.03%), Postives = 227/310 (73.23%), Query Frame = 0

Query: 4   VSLSLLSHSPSVLLLP----------SPSLQLSRPILLPPRTLPSCSSTSISSVSIT-SS 63
           ++L LLSH   +   P          S    LS+P+   P  + +  S+S+SS S + SS
Sbjct: 1   MALLLLSHCSILSFQPPLSSSSSFHSSHIQSLSKPVFASPSPIRNSISSSVSSSSSSVSS 60

Query: 64  SILPPKVVVTRERGKNGKLVSALAKHGINCLELPLIQHRQGPDLNRLQSVLSDSVFDWIV 123
           S   P+VVVTRERGKN +++ AL K+GI+ LELPLIQH +GPD +RL SVL+D  FDWI+
Sbjct: 61  SNSIPQVVVTRERGKNNQIIKALEKNGISSLELPLIQHARGPDFDRLASVLNDKSFDWII 120

Query: 124 ITSPEAGLVFLEAWKAAGMPKVRIGVVGAGTANIFEEVLQSSKQQLDVAFAPSKAIGKVL 183
           ITSPEAG VFLEAWK A  P+V+IGVVGAGTA +FEE ++S+   L VAF PSKA GKVL
Sbjct: 121 ITSPEAGSVFLEAWKTASSPEVQIGVVGAGTARVFEEAMKSADGLLHVAFTPSKATGKVL 180

Query: 184 AYELPKLGNTKCSVLYPASTKASNDIEEGLANRGFEVTRLNTYTTAPVDYVDQSILEQAL 243
           A ELP+    + SVLYPAS KA NDI EGL+ RGFEV RLNTYTT PV  VD  +L+QAL
Sbjct: 181 ASELPEKVGKRSSVLYPASLKAGNDIVEGLSKRGFEVVRLNTYTTVPVQSVDTVLLQQAL 240

Query: 244 LLPVVAVASPSAVRAWINLISEPKEWENSLACIGETTAAVAKRLGLKNVYYPKNPCLEGW 303
             PV++VASPSAVRAW++LI   ++W N +ACIGETTA+ A+RLGLKNVYYP+ P LEGW
Sbjct: 241 SAPVLSVASPSAVRAWLHLIQNEEQWSNYVACIGETTASAARRLGLKNVYYPEKPGLEGW 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O487212.0e-9259.03Uroporphyrinogen-III synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q10QR93.2e-9060.28Uroporphyrinogen-III synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=... [more]
Match NameE-valueIdentityDescription
XP_023537361.12.61e-212100.00uroporphyrinogen-III synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. p... [more]
KAG6585644.14.46e-20698.70Uroporphyrinogen-III synthase, chloroplastic, partial [Cucurbita argyrosperma su... [more]
XP_022951382.13.66e-20598.38uroporphyrinogen-III synthase, chloroplastic [Cucurbita moschata][more]
XP_023002006.19.28e-20196.75uroporphyrinogen-III synthase, chloroplastic-like [Cucurbita maxima][more]
KGN62543.13.26e-17085.76hypothetical protein Csa_021895 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1GHG91.77e-20598.38Uroporphyrinogen-III synthase OS=Cucurbita moschata OX=3662 GN=LOC111454223 PE=3... [more]
A0A6J1KK694.49e-20196.75Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111496019 PE=3 S... [more]
A0A0A0LLB21.58e-17085.76Uroporphyrinogen-III synthase OS=Cucumis sativus OX=3659 GN=Csa_2G360600 PE=3 SV... [more]
A0A6J1KA582.19e-17084.79Uroporphyrinogen-III synthase OS=Cucurbita maxima OX=3661 GN=LOC111492039 PE=3 S... [more]
A0A6J1CQT71.53e-16984.24Uroporphyrinogen-III synthase OS=Momordica charantia OX=3673 GN=LOC111013898 PE=... [more]
Match NameE-valueIdentityDescription
AT2G26540.11.4e-9359.03uroporphyrinogen-III synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003754Tetrapyrrole biosynthesis, uroporphyrinogen III synthasePFAMPF02602HEM4coord: 70..293
e-value: 1.2E-46
score: 159.0
IPR003754Tetrapyrrole biosynthesis, uroporphyrinogen III synthaseCDDcd06578HemDcoord: 59..296
e-value: 1.08029E-44
score: 150.535
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilyGENE3D3.40.50.10090coord: 210..301
e-value: 7.5E-8
score: 34.2
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilyGENE3D3.40.50.10090coord: 103..209
e-value: 1.5E-18
score: 68.9
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilySUPERFAMILY69618HemD-likecoord: 58..298
IPR039793Uroporphyrinogen-III synthasePANTHERPTHR38042UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTICcoord: 16..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g04560.1Cp4.1LG07g04560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0006780 uroporphyrinogen III biosynthetic process
biological_process GO:0033014 tetrapyrrole biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004852 uroporphyrinogen-III synthase activity