Cp4.1LG07g03540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g03540
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMicrosomal prostaglandin E synthase 2
LocationCp4.1LG07: 2282500 .. 2286544 (-)
RNA-Seq ExpressionCp4.1LG07g03540
SyntenyCp4.1LG07g03540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACCACACAACGGGGGGGGTTGTGATCTACTGCTTCCCGGGCTACCGTAATCCTCTGCACTCTCCTTACATCGACGGCTGAAATCGTACCAATTTCTCTTCCCCCAAACCCTTGTAAAACCTTGCAAAAGAGTTCAACTTCCAGAGAACTGTCGGTCGCCATGAGGAGGATTAATGGAGTTCGCGTACTCAGCCGGTTTGTAAGCGGTGAGGCCAACACCGCCACTGTCACCGCTGATCGGCGGCTACTCCATCCATCGGCGGTTCACAGAACGTTCATTGGATCTGATATTCGGTGTTTCTCGCAAGCGAAGAATCCATTTGGCTTGTATTGTCCTTCTTTTGCTCGGAATGTTGCAGGGAATGGTCGGTGCTTCTCTGTGGCCTCTTCATCTTCGGTTCATGATGTGGTTAATGACCTACCTCGTTTGTCGTTCGCACCCAAGGATGTGGTTCTCTATCAATACGAGGCTTGCCCGTTCTGCAACAAAGTTAAAGGTAATTAGCATAATCTCTTAGAGGAATTCTCAGAATATGAAGTTTTTGTCAACTTGATCTTGATAAGGAAAATAGAGAACGTGGATTAAGGACTGGACAATAGATCATATATTACTGTTACCTATGAGCTGAAGCTAGAATTCTTGTCGTGAAGTTGCAATTCTATGCCATTTTTCCTTTCTAGGTCCTTAAAATTTTTGTTGATTTTCCTTTTCCTCCAGCATTCTTGGATTATTACAATGTTCCTTATAAAGTCGTGGAGGTGAATCCCATATTCAAAAAGGAGATTAAGTGGTCTGAATACAAGAAGGTTCCAATTCTGATGGTTGATGGTGTTCAAATGGTGGATTCTACAGGTTCAGTCAAATCTGTAATTACTTGGAAAATGGAGAATACATACATACATATATATCTATACACACACACACACACACACACACACACACACACATATATATATATGTTTTCAATTAGAGTAGTTGTTGATGATCGGGGCCTGTCTTGCTTGTTCTTGTACAGATATAACTGATAAGTTGTACAAAAGAATTCATCCAGAGAATTCTACCTTAATTCTATCAAATAATTTGAAATTAAATAATAATTAAACGGTAAGAGCCCAAAATATAAGAAAATAATATTTACAAACATAATTAAAAAAAATAAAATAGTATTAAACTACCTTTAAAATTAAAATAAAAAATAAAAAAAAAAAGTGAAATATCGAATTTATATATTCAGAAATATATATATACAGAAAGGGCTTTTTGGGAAATACATTACCATGGTAGGGGGTGTTTGACGCGTTACCACACAACGGGGGGGGTGTGATCTACTGCTTCCCGGGCTACCGTAATCCTCTGCACTCTCCTTACATCGACGGCTGAAATCGTACCAATTTCTCTTCCCCCAAACCCTTGTAAAACCTTGCAAAAGAGTTCAACTTCCAGAGAACTGTCGGTCGCCATGAGGAGGATTAATGGAGTTCGCGTACTCAGCCGGTTTGTAAGCGGTGAGGCCAACACCGCCACTGTCACCGCTGATCGGCGGCTACTCCATCCATCGGCGGTTCACAGAACGTTCATTGGATCTGATATTCGGTGTTTCTCGCAAGCGAAGAATCCATTTGGCTTGTATTGTCATTCTTTTGCTCGGAATGTTGCAGGGAATGGTCGGTGCTTCTCTGTGGCCTCTTCATCTTCGGTTCATGATGTGGTTAATGACCTACCTCGTTTGTCGTTCGCACCCAAGGATGTGGTTCTCTATCAATACGAGGCTTGCCCGTTCTGCAACAAAGTTAAAGGTAATTAGCATAATCTCTTAGAGGAATTCTCAGAATATGAAGTTTTTGTCAACTTGATCTTGATAAGGAAAATAGAGAAGATGGATTAAGGACTGGACAATAGATCATATATCACCTATGAGCTGAAGCTAGAATTCTTGTCGTGAATTTGCAGTTCTATGCCATTCTTCCTTTCTAGATCCTTAAAAATTTCTGTAAATTTTCTTTTTCCTCCAGCATTCTTGGATTATTACAATGTTCCTTATAAAGTTGTGGAGGTGAATCCCATATTCAAAAAGGAGATTAAGTGGTCTGAATACAAGAAGGTTCCAATTCTGATGGTTGATGGTGTTCAAATGGTGGATTCTACAGGTTCAGTAATCTGTAATTACTTGGAAAATGGAGAATACATACATACATATCTATATATATATATATACATATCTCTATATATGTGTGTGTATATATATATATATATATATATATATGTCTTCAATTAGAGTAGTTGTTGATGATCAGGGCCTGTCTTGCTTGTTCTTGTACAGATATAATTGATAAGTTGTACAAAAGAATTCATCCAGAGAATTCTACCTCGGACTCAGAGGAGGAGAGGAAGTGGCTTGGGTATACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTTACATCTCGAGGCTCTATACTTGCTTACAAGCAGCTATTATATGGACCCAATTGAGACTTTAAGGGAAACCAGCGTCTTCATTGCCAAAGAATTTGACTGACTAATTTGTCTTTCGACATTTTTGTGTATCATATGTAACTGATTGATGAGCATATATTATGTAGATGGGTGGACAATCACCTGGTGCACGTTTTATCCCCGAACATTTACAGAAATTACAAGGAGGCTCTCGAGTCTTTCAACTACATAACTACACATGGTATGATAGTAAATCAATTTATGAGCTGCTCTGTTTCCCCCCCTCCCTAGTTCTTTGTAATCTTGTCTATTCATATTGAATTATATGAGCTGGAGACGGTTTCTAGGGCCCTGTTTGTTAACAATTTGATTTTTGGTTTTTGAACATTAAGCTGTTAAACGGTATTATATATGAGCTGGAGACTCATTGGTTTATAGGGCCCCGTTTGATAACAATTTGATTTTTAAACATTAAGTTGCTAAACGGTATTATATACGAGCTGGAGACTCATTGGTTTCTTAGGTCCTGTTTGTTAACAATTTGATTTTTGGTTTTTAAACGTTAAGCTGTTAAACGGTATTATCACTTGTGGGTTTATTTCTTTTCTATTTTTTTCCAATGTTTTCAAAATCCAAGCCAAGTTTTGAATACTCACTTTCATATATTTTCAATGTTCACTATATAATTTGGATACCTTATTCCAATTATTATATTCATAAGAAAACATCCTCAACATGATTTTTTGTTTGTTTGTTTGTTTGTTGTTCTTGTTGTTGAAGCAATCGCATATATTCATGAGCTCCTGACTTGTCTGTATAACTTTAATATGGTGCAGGAAATTTTAGTTTCACTCAAAGGATAATAGCTAAGTATGGCGGGGCTACAGCCATGTATTTTGTTTCCAAGAAATTGAAAGAAAAACATAACATCACTGATGAACGAAAAGCTTTATATGGAGCTGCAGAAACATGGGTGGAAGCACTGAAAGACCGTCAGTTTCTTGGTATGCTTAACTTCATTCACGATTCTGTTCAGTTATTTAGGTTCNAAGACCGTCAGTTTCTTGGTATGCTTAACTTCATTCACGATTCTGTTCGGTTATTTAGGTTTAAGCTAAAGGTATTTGAGAAAAACAGTTCTATTTTACGACGAATTTGTCTCTCGATAGGTGGACCAAATCCTAATTTAGCTGATCTTGCTGTCTTTGGCGTCTTGAGACCAATCCGACACCTTCAATCTGGAATAGATATGGTAGAAAATACAAGAGTTGGCGAATGGTATTCTAGAATGGAGGATGCTGTAGGAGAGTCTGCAAGGATCGTCTCTTGAAGTTAGTACAATTTATAGTTTTTGTTTTTGATTACTCAATATCCTGCCAGTTTATTGATAAATATCTGAACTTTTTTTAACATCAGATCCTAAAAGATTGTCTGTGAGAATAATCTGCTAGATCCATGTAGCGAATTGCAAATTTCTCATCCTTTTAGATGATTGCTTCTATATTTGTATTTAATGTGTTGATTCCTTGTTCTACTTAACTGTATTGTGAACAACCTAAATGGTATCAAAGTGTTGACAGAACA

mRNA sequence

TACCACACAACGGGGGGGGTTGTGATCTACTGCTTCCCGGGCTACCGTAATCCTCTGCACTCTCCTTACATCGACGGCTGAAATCGTACCAATTTCTCTTCCCCCAAACCCTTGTAAAACCTTGCAAAAGAGTTCAACTTCCAGAGAACTGTCGGTCGCCATGAGGAGGATTAATGGAGTTCGCGTACTCAGCCGGTTTGTAAGCGGTGAGGCCAACACCGCCACTGTCACCGCTGATCGGCGGCTACTCCATCCATCGGCGGTTCACAGAACGTTCATTGGATCTGATATTCGGTGTTTCTCGCAAGCGAAGAATCCATTTGGCTTGTATTGTCCTTCTTTTGCTCGGAATGTTGCAGGGAATGGTCGGTGCTTCTCTGTGGCCTCTTCATCTTCGGTTCATGATGTGGTTAATGACCTACCTCGTTTGTCGTTCGCACCCAAGGATGTGGTTCTCTATCAATACGAGGCTTGCCCGTTCTGCAACAAAGTTAAAGCATTCTTGGATTATTACAATGTTCCTTATAAAGTCGTGGAGGTGAATCCCATATTCAAAAAGGAGATTAAGTGGTCTGAATACAAGAAGGTTCCAATTCTGATGGTTGATGGTGTTCAAATGGTGGATTCTACAGGTTCAGTCAAATCTAGTTCAACTTCCAGAGAACTGTCGGTCGCCATGAGGAGGATTAATGGAGTTCGCGTACTCAGCCGGTTTGTAAGCGGTGAGGCCAACACCGCCACTGTCACCGCTGATCGGCGGCTACTCCATCCATCGGCGGTTCACAGAACGTTCATTGGATCTGATATTCGGTGTTTCTCGCAAGCGAAGAATCCATTTGGCTTGTATTGTCATTCTTTTGCTCGGAATGTTGCAGGGAATGGTCGGTGCTTCTCTGTGGCCTCTTCATCTTCGGTTCATGATGTGGTTAATGACCTACCTCGTTTGTCGTTCGCACCCAAGGATGTGGTTCTCTATCAATACGAGGCTTGCCCGTTCTGCAACAAAGTTAAAGCATTCTTGGATTATTACAATGTTCCTTATAAAGTTGTGGAGGTGAATCCCATATTCAAAAAGGAGATTAAGTGGTCTGAATACAAGAAGGTTCCAATTCTGATGGTTGATGGTGTTCAAATGGTGGATTCTACAGATATAATTGATAAGTTGTACAAAAGAATTCATCCAGAGAATTCTACCTCGGACTCAGAGGAGGAGAGGAAGTGGCTTGGATGGGTGGACAATCACCTGGTGCACGTTTTATCCCCGAACATTTACAGAAATTACAAGGAGGCTCTCGAGTCTTTCAACTACATAACTACACATGGAAATTTTAGTTTCACTCAAAGGATAATAGCTAAGTATGGCGGGGCTACAGCCATGTATTTTGTTTCCAAGAAATTGAAAGAAAAACATAACATCACTGATGAACGAAAAGCTTTATATGGAGCTGCAGAAACATGGGTGGAAGCACTGAAAGACCGTCAGTTTCTTGGTATGCTTAACTTCATTCACGATTCTGTTCAGTTATTTAGGTTCNAAGACCGTCAGTTTCTTGGTGGACCAAATCCTAATTTAGCTGATCTTGCTGTCTTTGGCGTCTTGAGACCAATCCGACACCTTCAATCTGGAATAGATATGGTAGAAAATACAAGAGTTGGCGAATGGTATTCTAGAATGGAGGATGCTGTAGGAGAGTCTGCAAGGATCGTCTCTTGAAGTTAGTACAATTTATAGTTTTTGTTTTTGATTACTCAATATCCTGCCAGTTTATTGATAAATATCTGAACTTTTTTTAACATCAGATCCTAAAAGATTGTCTGTGAGAATAATCTGCTAGATCCATGTAGCGAATTGCAAATTTCTCATCCTTTTAGATGATTGCTTCTATATTTGTATTTAATGTGTTGATTCCTTGTTCTACTTAACTGTATTGTGAACAACCTAAATGGTATCAAAGTGTTGACAGAACA

Coding sequence (CDS)

ATGAGGAGGATTAATGGAGTTCGCGTACTCAGCCGGTTTGTAAGCGGTGAGGCCAACACCGCCACTGTCACCGCTGATCGGCGGCTACTCCATCCATCGGCGGTTCACAGAACGTTCATTGGATCTGATATTCGGTGTTTCTCGCAAGCGAAGAATCCATTTGGCTTGTATTGTCCTTCTTTTGCTCGGAATGTTGCAGGGAATGGTCGGTGCTTCTCTGTGGCCTCTTCATCTTCGGTTCATGATGTGGTTAATGACCTACCTCGTTTGTCGTTCGCACCCAAGGATGTGGTTCTCTATCAATACGAGGCTTGCCCGTTCTGCAACAAAGTTAAAGCATTCTTGGATTATTACAATGTTCCTTATAAAGTCGTGGAGGTGAATCCCATATTCAAAAAGGAGATTAAGTGGTCTGAATACAAGAAGGTTCCAATTCTGATGGTTGATGGTGTTCAAATGGTGGATTCTACAGGTTCAGTCAAATCTAGTTCAACTTCCAGAGAACTGTCGGTCGCCATGAGGAGGATTAATGGAGTTCGCGTACTCAGCCGGTTTGTAAGCGGTGAGGCCAACACCGCCACTGTCACCGCTGATCGGCGGCTACTCCATCCATCGGCGGTTCACAGAACGTTCATTGGATCTGATATTCGGTGTTTCTCGCAAGCGAAGAATCCATTTGGCTTGTATTGTCATTCTTTTGCTCGGAATGTTGCAGGGAATGGTCGGTGCTTCTCTGTGGCCTCTTCATCTTCGGTTCATGATGTGGTTAATGACCTACCTCGTTTGTCGTTCGCACCCAAGGATGTGGTTCTCTATCAATACGAGGCTTGCCCGTTCTGCAACAAAGTTAAAGCATTCTTGGATTATTACAATGTTCCTTATAAAGTTGTGGAGGTGAATCCCATATTCAAAAAGGAGATTAAGTGGTCTGAATACAAGAAGGTTCCAATTCTGATGGTTGATGGTGTTCAAATGGTGGATTCTACAGATATAATTGATAAGTTGTACAAAAGAATTCATCCAGAGAATTCTACCTCGGACTCAGAGGAGGAGAGGAAGTGGCTTGGATGGGTGGACAATCACCTGGTGCACGTTTTATCCCCGAACATTTACAGAAATTACAAGGAGGCTCTCGAGTCTTTCAACTACATAACTACACATGGAAATTTTAGTTTCACTCAAAGGATAATAGCTAAGTATGGCGGGGCTACAGCCATGTATTTTGTTTCCAAGAAATTGAAAGAAAAACATAACATCACTGATGAACGAAAAGCTTTATATGGAGCTGCAGAAACATGGGTGGAAGCACTGAAAGACCGTCAGTTTCTTGGTATGCTTAACTTCATTCACGATTCTGTTCAGTTATTTAGGTTCNAAGACCGTCAGTTTCTTGGTGGACCAAATCCTAATTTAGCTGATCTTGCTGTCTTTGGCGTCTTGAGACCAATCCGACACCTTCAATCTGGAATAGATATGGTAGAAAATACAAGAGTTGGCGAATGGTATTCTAGAATGGAGGATGCTGTAGGAGAGTCTGCAAGGATCGTCTCTTGA

Protein sequence

MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCPSFARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTGSVKSSSTSRELSVAMRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHSFARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKKLKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLADLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS
Homology
BLAST of Cp4.1LG07g03540 vs. ExPASy Swiss-Prot
Match: Q7ZUC7 (Prostaglandin E synthase 2 OS=Danio rerio OX=7955 GN=ptges2 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.7e-54
Identity = 122/286 (42.66%), Postives = 162/286 (56.64%), Query Frame = 0

Query: 269 VVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMVDG-VQMVD 328
           + LYQY+ CPFC+KV+AFLDY+ +PY++VEVNP+ ++EIKWS Y+KVPILMV+G VQ+ D
Sbjct: 107 LTLYQYKTCPFCSKVRAFLDYHRLPYEIVEVNPVMRQEIKWSTYRKVPILMVNGTVQLND 166

Query: 329 STDIIDKLYKRI-------------HPENSTSDS-------------------------- 388
           S+ II  L   I             +PE  + +                           
Sbjct: 167 SSVIISALKTYISSKDKKISEILACYPEMKSKNDRGKDVIEFGNKYWVMVHDADADQLYP 226

Query: 389 -----EEERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGA 448
                +EE KW  W D+ LVH++SPN+YR   EAL SF+YI   G F   +   AKY GA
Sbjct: 227 GKDSRKEEIKWRTWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGSFEGFFAKYFGA 286

Query: 449 TAMYFVSKKLKEKHNI-TDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQ 508
            AM+ +SK+LK KHN+  D R+ LY A   WV A+                      ++Q
Sbjct: 287 AAMWIISKRLKYKHNLQADVRQDLYKAVNDWVAAIG--------------------KNKQ 346

BLAST of Cp4.1LG07g03540 vs. ExPASy Swiss-Prot
Match: Q66LN0 (Prostaglandin E synthase 2 OS=Bos taurus OX=9913 GN=PTGES2 PE=1 SV=3)

HSP 1 Score: 196.4 bits (498), Expect = 7.7e-49
Identity = 110/292 (37.67%), Postives = 158/292 (54.11%), Query Frame = 0

Query: 269 VVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMV---DGVQM 328
           + LYQY+ CPFC+KV+AFLD++ +PY+VVEVNP+ + EIK+S Y+KVPI+M    + +Q 
Sbjct: 99  LTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIKFSSYRKVPIVMAQEGESLQQ 158

Query: 329 VDSTDIIDKLYKR--------------IHPENSTSDS----------------------- 388
           ++ + +I    K                 P  + +D                        
Sbjct: 159 LNDSSVIISALKTYLVSGQPLADIITYYPPMKAVNDQGKEVTEFCNKYWLMLDEKEAQRM 218

Query: 389 -------EEERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYG 448
                   EE KW  W D+ LVH++SPN+YR   EAL SF+YI   GNF   +  +AKY 
Sbjct: 219 YGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVKEGNFGTVEGAMAKYM 278

Query: 449 GATAMYFVSKKLKEKHNITDE-RKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXD 508
           GA AMYF+SK+LK +H++ D+ R+ LY AA  WV A+                      D
Sbjct: 279 GAAAMYFISKRLKRRHHLRDDVREDLYEAANKWVAAVG--------------------KD 338

Query: 509 RQFLGGPNPNLADLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGES 513
           R F+GG  PNLADLAV+GVLR +  L++  D++ +T +  WY R+E A+ E+
Sbjct: 339 RPFMGGQKPNLADLAVYGVLRVMEGLEAFDDLMRHTHIQPWYLRVEKAIAEA 370

BLAST of Cp4.1LG07g03540 vs. ExPASy Swiss-Prot
Match: Q8BWM0 (Prostaglandin E synthase 2 OS=Mus musculus OX=10090 GN=Ptges2 PE=1 SV=3)

HSP 1 Score: 192.6 bits (488), Expect = 1.1e-47
Identity = 108/295 (36.61%), Postives = 159/295 (53.90%), Query Frame = 0

Query: 269 VVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMV---DGVQM 328
           + LYQY+ CPFC+KV+AFLD++++PY+VVEVNP+ + EIK+S Y+KVPIL+    D +Q 
Sbjct: 101 LTLYQYKTCPFCSKVRAFLDFHSLPYQVVEVNPVRRTEIKFSSYRKVPILVAQEGDSLQQ 160

Query: 329 VDSTDII--------------DKLYKRIHPENSTSDS----------------------- 388
           ++ + +I              +++     P  + +D                        
Sbjct: 161 LNDSSVIISALKTYLVSGQPLEEVITYYPPMKAMNDQGKEVTEFCNKYWLMLDEKEAQQM 220

Query: 389 -------EEERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYG 448
                   EE KW  W D+ LVH++SPN+YR   EAL SF+YI   G F   +  +AKY 
Sbjct: 221 YGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVREGKFGAVEAAMAKYV 280

Query: 449 GATAMYFVSKKLKEKHNITDE-RKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXD 508
           GA AMY +SK+LK +H++ D+ R  LY AA  WV A+                      D
Sbjct: 281 GAAAMYLISKRLKSRHHLQDDVRVDLYEAANKWVTAVG--------------------KD 340

Query: 509 RQFLGGPNPNLADLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARI 516
           R F+GG  PNLADLAV+GVLR +  L++  D++ ++ +  WY RME A+ E+  +
Sbjct: 341 RPFMGGQKPNLADLAVYGVLRVMEGLEAFDDLMRHSHIQPWYLRMERAIEEAPSV 375

BLAST of Cp4.1LG07g03540 vs. ExPASy Swiss-Prot
Match: Q9N0A4 (Prostaglandin E synthase 2 OS=Macaca fascicularis OX=9541 GN=PTGES2 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.5e-47
Identity = 111/293 (37.88%), Postives = 154/293 (52.56%), Query Frame = 0

Query: 269 VVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMV----DGVQ 328
           + LYQY+ CPFC+KV+AFLD++ +PY+VVEVNP+ + EIK+S Y+KVPIL+        Q
Sbjct: 102 LTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVLRAEIKFSSYRKVPILVAQEGESSQQ 161

Query: 329 MVDSTDIIDKL----------------YKRIHPENSTSDS-------------------- 388
           + DS+ II  L                Y  +   N                         
Sbjct: 162 LNDSSVIISALKTYLVSGQPLEEIITYYPAMKAVNDQGKEVTEFGNKYWLMLNEKEAQQV 221

Query: 389 -------EEERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYG 448
                   EE KW  W D+ LVH++SPN+YR   EAL SF+YI   G F   +  +AKY 
Sbjct: 222 YSGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYM 281

Query: 449 GATAMYFVSKKLKEKHNITDE-RKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXD 508
           GA AMY +SK+LK +H + D  R+ LY AA+ WV A+                      D
Sbjct: 282 GAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVG--------------------KD 341

Query: 509 RQFLGGPNPNLADLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESA 514
           R F+GG  PNLADLAV+GVLR +  L +  D++++T +  WY R+E A+ E++
Sbjct: 342 RPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEAS 374

BLAST of Cp4.1LG07g03540 vs. ExPASy Swiss-Prot
Match: Q9H7Z7 (Prostaglandin E synthase 2 OS=Homo sapiens OX=9606 GN=PTGES2 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.3e-47
Identity = 111/293 (37.88%), Postives = 154/293 (52.56%), Query Frame = 0

Query: 269 VVLYQYEACPFCNKVKAFLDYYNVPYKVVEVNPIFKKEIKWSEYKKVPILMV----DGVQ 328
           + LYQY+ CPFC+KV+AFLD++ +PY+VVEVNP+ + EIK+S Y+KVPIL+        Q
Sbjct: 102 LTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIKFSSYRKVPILVAQEGESSQQ 161

Query: 329 MVDSTDIIDKL----------------YKRIHPENSTSDS-------------------- 388
           + DS+ II  L                Y  +   N                         
Sbjct: 162 LNDSSVIISALKTYLVSGQPLEEIITYYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQV 221

Query: 389 -------EEERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYG 448
                   EE KW  W D+ LVH++SPN+YR   EAL SF+YI   G F   +  +AKY 
Sbjct: 222 YGGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYM 281

Query: 449 GATAMYFVSKKLKEKHNITDE-RKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXD 508
           GA AMY +SK+LK +H + D  R+ LY AA+ WV A+                      D
Sbjct: 282 GAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVG--------------------KD 341

Query: 509 RQFLGGPNPNLADLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESA 514
           R F+GG  PNLADLAV+GVLR +  L +  D++++T +  WY R+E A+ E++
Sbjct: 342 RPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEAS 374

BLAST of Cp4.1LG07g03540 vs. NCBI nr
Match: XP_023536773.1 (prostaglandin E synthase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647 bits (1669), Expect = 6.77e-231
Identity = 324/345 (93.91%), Postives = 324/345 (93.91%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS
Sbjct: 1   MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNITDERKALYGAAETWVEALKDRQFLG                     GPNPNLA
Sbjct: 241 LKEKHNITDERKALYGAAETWVEALKDRQFLG---------------------GPNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS
Sbjct: 301 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 324

BLAST of Cp4.1LG07g03540 vs. NCBI nr
Match: KAG6585529.1 (Prostaglandin E synthase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 640 bits (1650), Expect = 5.27e-228
Identity = 321/345 (93.04%), Postives = 322/345 (93.33%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGVRVLSR VSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYC S
Sbjct: 1   MRRINGVRVLSRIVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNITDERKALYGAAETWVEALKDRQFLG                     GPNPNLA
Sbjct: 241 LKEKHNITDERKALYGAAETWVEALKDRQFLG---------------------GPNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGES+RIVS
Sbjct: 301 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESSRIVS 324

BLAST of Cp4.1LG07g03540 vs. NCBI nr
Match: XP_023002430.1 (prostaglandin E synthase 2-like [Cucurbita maxima])

HSP 1 Score: 633 bits (1632), Expect = 2.89e-225
Identity = 318/345 (92.17%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGVRVLSR VSGEANTATVTADRRLLHPSAVHRT IGSDIRCFSQ KNPFGLYC S
Sbjct: 1   MRRINGVRVLSRIVSGEANTATVTADRRLLHPSAVHRTSIGSDIRCFSQGKNPFGLYCPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           F RNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  FVRNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNI DERKALYGAAETWVEALKDRQFLG                     GPNPNLA
Sbjct: 241 LKEKHNIVDERKALYGAAETWVEALKDRQFLG---------------------GPNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS
Sbjct: 301 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 324

BLAST of Cp4.1LG07g03540 vs. NCBI nr
Match: XP_022951333.1 (prostaglandin E synthase 2-like [Cucurbita moschata])

HSP 1 Score: 625 bits (1613), Expect = 1.94e-222
Identity = 317/345 (91.88%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGVRVLSR VSGEANTATVTADRRLLHPSAVHR    SDIRCFSQ KNPFGLYC S
Sbjct: 1   MRRINGVRVLSRIVSGEANTATVTADRRLLHPSAVHR----SDIRCFSQGKNPFGLYCPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNITDERKALYGAAETWVEALKDRQFLG                     GPNPNLA
Sbjct: 241 LKEKHNITDERKALYGAAETWVEALKDRQFLG---------------------GPNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS
Sbjct: 301 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 320

BLAST of Cp4.1LG07g03540 vs. NCBI nr
Match: XP_022144146.1 (prostaglandin E synthase 2-like [Momordica charantia])

HSP 1 Score: 538 bits (1387), Expect = 4.61e-188
Identity = 275/345 (79.71%), Postives = 292/345 (84.64%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGV +LSR VSG+A TA     R LL  SAVHRT  GSDIRCF Q  +PFGLY  S
Sbjct: 1   MRRINGVHLLSRIVSGDATTANY---RPLLRQSAVHRTRSGSDIRCFLQGVSPFGLYDLS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           F RNVAGN RCFSVASSS + +VV+D PR SF PK+VVLYQYEACPFCNKVKAFLDYYN+
Sbjct: 61  FVRNVAGNSRCFSVASSSMLQEVVHDPPRSSFVPKEVVLYQYEACPFCNKVKAFLDYYNI 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWS YKKVPILMVDGVQMVDSTDIIDKLY+ IHPENSTS+SEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSAYKKVPILMVDGVQMVDSTDIIDKLYQGIHPENSTSNSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRN+KEALESFNYITTHGNFSF QRII+KYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNFKEALESFNYITTHGNFSFIQRIISKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKE+HNI DERKALYGAAETWV+ALKDRQFLG                     G NPNLA
Sbjct: 241 LKERHNIKDERKALYGAAETWVDALKDRQFLG---------------------GSNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIR+LQSGIDMVE+TRVGEWY+RME AVGESARI S
Sbjct: 301 DLAVFGVLRPIRYLQSGIDMVEHTRVGEWYTRMEKAVGESARIAS 321

BLAST of Cp4.1LG07g03540 vs. ExPASy TrEMBL
Match: A0A6J1KNY1 (Microsomal prostaglandin E synthase 2 OS=Cucurbita maxima OX=3661 GN=LOC111496273 PE=3 SV=1)

HSP 1 Score: 633 bits (1632), Expect = 1.40e-225
Identity = 318/345 (92.17%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGVRVLSR VSGEANTATVTADRRLLHPSAVHRT IGSDIRCFSQ KNPFGLYC S
Sbjct: 1   MRRINGVRVLSRIVSGEANTATVTADRRLLHPSAVHRTSIGSDIRCFSQGKNPFGLYCPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           F RNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  FVRNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNI DERKALYGAAETWVEALKDRQFLG                     GPNPNLA
Sbjct: 241 LKEKHNIVDERKALYGAAETWVEALKDRQFLG---------------------GPNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS
Sbjct: 301 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 324

BLAST of Cp4.1LG07g03540 vs. ExPASy TrEMBL
Match: A0A6J1GHF3 (Microsomal prostaglandin E synthase 2 OS=Cucurbita moschata OX=3662 GN=LOC111454196 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 9.37e-223
Identity = 317/345 (91.88%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGVRVLSR VSGEANTATVTADRRLLHPSAVHR    SDIRCFSQ KNPFGLYC S
Sbjct: 1   MRRINGVRVLSRIVSGEANTATVTADRRLLHPSAVHR----SDIRCFSQGKNPFGLYCPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNITDERKALYGAAETWVEALKDRQFLG                     GPNPNLA
Sbjct: 241 LKEKHNITDERKALYGAAETWVEALKDRQFLG---------------------GPNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS
Sbjct: 301 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 320

BLAST of Cp4.1LG07g03540 vs. ExPASy TrEMBL
Match: A0A6J1CSF9 (Microsomal prostaglandin E synthase 2 OS=Momordica charantia OX=3673 GN=LOC111013903 PE=3 SV=1)

HSP 1 Score: 538 bits (1387), Expect = 2.23e-188
Identity = 275/345 (79.71%), Postives = 292/345 (84.64%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGV +LSR VSG+A TA     R LL  SAVHRT  GSDIRCF Q  +PFGLY  S
Sbjct: 1   MRRINGVHLLSRIVSGDATTANY---RPLLRQSAVHRTRSGSDIRCFLQGVSPFGLYDLS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
           F RNVAGN RCFSVASSS + +VV+D PR SF PK+VVLYQYEACPFCNKVKAFLDYYN+
Sbjct: 61  FVRNVAGNSRCFSVASSSMLQEVVHDPPRSSFVPKEVVLYQYEACPFCNKVKAFLDYYNI 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWS YKKVPILMVDGVQMVDSTDIIDKLY+ IHPENSTS+SEEER
Sbjct: 121 PYKVVEVNPIFKKEIKWSAYKKVPILMVDGVQMVDSTDIIDKLYQGIHPENSTSNSEEER 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRN+KEALESFNYITTHGNFSF QRII+KYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNFKEALESFNYITTHGNFSFIQRIISKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKE+HNI DERKALYGAAETWV+ALKDRQFLG                     G NPNLA
Sbjct: 241 LKERHNIKDERKALYGAAETWVDALKDRQFLG---------------------GSNPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARIVS 517
           DLAVFGVLRPIR+LQSGIDMVE+TRVGEWY+RME AVGESARI S
Sbjct: 301 DLAVFGVLRPIRYLQSGIDMVEHTRVGEWYTRMEKAVGESARIAS 321

BLAST of Cp4.1LG07g03540 vs. ExPASy TrEMBL
Match: A0A5A7VH91 (Microsomal prostaglandin E synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001510 PE=3 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 2.86e-182
Identity = 271/343 (79.01%), Postives = 283/343 (82.51%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGV +L R VSG+A T T T  R+LL  SAV RT  GS+IRCFSQ  NPFG Y  S
Sbjct: 1   MRRINGVHLLGRLVSGDATTTTSTY-RQLLRQSAVLRTCTGSNIRCFSQVANPFGSYDPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
             R VAGN R  SV SSS   D+VN  PR SF PKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  SVRKVAGNARFVSVTSSSLAEDLVNGSPRPSFVPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDII KL++RIHPENS S+ EEE+
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIHKLHQRIHPENSGSNLEEEK 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSF QRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFAQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNITDERKALYGAAETWV+ALKDRQFLG                     G NPNLA
Sbjct: 241 LKEKHNITDERKALYGAAETWVDALKDRQFLG---------------------GANPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARI 515
           DLAVFGVLRPIRHLQSG DMVE+TRVGEWY+RME AVGESARI
Sbjct: 301 DLAVFGVLRPIRHLQSGKDMVEHTRVGEWYTRMEKAVGESARI 321

BLAST of Cp4.1LG07g03540 vs. ExPASy TrEMBL
Match: A0A1S3BDA9 (Microsomal prostaglandin E synthase 2 OS=Cucumis melo OX=3656 GN=LOC103488396 PE=3 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 2.86e-182
Identity = 271/343 (79.01%), Postives = 283/343 (82.51%), Query Frame = 0

Query: 173 MRRINGVRVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYCHS 232
           MRRINGV +L R VSG+A T T T  R+LL  SAV RT  GS+IRCFSQ  NPFG Y  S
Sbjct: 1   MRRINGVHLLGRLVSGDATTTTSTY-RQLLRQSAVLRTCTGSNIRCFSQVANPFGSYDPS 60

Query: 233 FARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYYNV 292
             R VAGN R  SV SSS   D+VN  PR SF PKDVVLYQYEACPFCNKVKAFLDYYNV
Sbjct: 61  SVRKVAGNARFVSVTSSSLAEDLVNGSPRPSFVPKDVVLYQYEACPFCNKVKAFLDYYNV 120

Query: 293 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEEER 352
           PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDII KL++RIHPENS S+ EEE+
Sbjct: 121 PYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIHKLHQRIHPENSGSNLEEEK 180

Query: 353 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVSKK 412
           KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSF QRIIAKYGGATAMYFVSKK
Sbjct: 181 KWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFAQRIIAKYGGATAMYFVSKK 240

Query: 413 LKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPNLA 472
           LKEKHNITDERKALYGAAETWV+ALKDRQFLG                     G NPNLA
Sbjct: 241 LKEKHNITDERKALYGAAETWVDALKDRQFLG---------------------GANPNLA 300

Query: 473 DLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARI 515
           DLAVFGVLRPIRHLQSG DMVE+TRVGEWY+RME AVGESARI
Sbjct: 301 DLAVFGVLRPIRHLQSGKDMVEHTRVGEWYTRMEKAVGESARI 321

BLAST of Cp4.1LG07g03540 vs. TAIR 10
Match: AT5G42150.1 (Glutathione S-transferase family protein )

HSP 1 Score: 375.2 bits (962), Expect = 8.6e-104
Identity = 202/345 (58.55%), Postives = 237/345 (68.70%), Query Frame = 0

Query: 173 MRRINGV--RVLSRFVSGEANTATVTADRRLLHPSAVHRTFIGSDIRCFSQAKNPFGLYC 232
           MRR+ G+  R +S  V+  +      A   +     + R F G       + K P     
Sbjct: 1   MRRVTGLAARTISSSVAINSRLTQSMAITTISSSEPISRRFGG-----LPEIKTP----- 60

Query: 233 HSFARNVAGNGRCFSVASSSSVHDVVNDLPRLSFAPKDVVLYQYEACPFCNKVKAFLDYY 292
            SFA  VAG     + A SS   +V        F PK+VVLYQYEACPFCNKVKAFLDY 
Sbjct: 61  -SFAGGVAGVVFFSAAAVSSLGQEVHAKEMAQKFNPKEVVLYQYEACPFCNKVKAFLDYN 120

Query: 293 NVPYKVVEVNPIFKKEIKWSEYKKVPILMVDGVQMVDSTDIIDKLYKRIHPENSTSDSEE 352
            +PYKVVEVNPI KKEIKWS+YKKVPIL VDG QMVDS+ IID L++++HPE S S+ +E
Sbjct: 121 KIPYKVVEVNPISKKEIKWSDYKKVPILTVDGEQMVDSSVIIDSLFQKMHPEISKSEDDE 180

Query: 353 ERKWLGWVDNHLVHVLSPNIYRNYKEALESFNYITTHGNFSFTQRIIAKYGGATAMYFVS 412
           E KW  WVDNHLVH+LSPNIYRN  EALESF YITTHGNFSFT+R++AKY GATAMYFVS
Sbjct: 181 ETKWRKWVDNHLVHLLSPNIYRNTSEALESFEYITTHGNFSFTERLVAKYAGATAMYFVS 240

Query: 413 KKLKEKHNITDERKALYGAAETWVEALKDRQFLGMLNFIHDSVQLFRFXDRQFLGGPNPN 472
           KKLK+K+NITDER ALY AAETWV+ALK                     +R +LGG  PN
Sbjct: 241 KKLKKKYNITDERAALYDAAETWVDALK---------------------ERPYLGGSKPN 300

Query: 473 LADLAVFGVLRPIRHLQSGIDMVENTRVGEWYSRMEDAVGESARI 516
           L DLAVFGVLRPIR+L+SG DMV+NTR+GEWYSRME+ VGE +RI
Sbjct: 301 LGDLAVFGVLRPIRYLRSGKDMVDNTRIGEWYSRMENTVGEPSRI 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7ZUC72.7e-5442.66Prostaglandin E synthase 2 OS=Danio rerio OX=7955 GN=ptges2 PE=2 SV=1[more]
Q66LN07.7e-4937.67Prostaglandin E synthase 2 OS=Bos taurus OX=9913 GN=PTGES2 PE=1 SV=3[more]
Q8BWM01.1e-4736.61Prostaglandin E synthase 2 OS=Mus musculus OX=10090 GN=Ptges2 PE=1 SV=3[more]
Q9N0A41.5e-4737.88Prostaglandin E synthase 2 OS=Macaca fascicularis OX=9541 GN=PTGES2 PE=1 SV=1[more]
Q9H7Z73.3e-4737.88Prostaglandin E synthase 2 OS=Homo sapiens OX=9606 GN=PTGES2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023536773.16.77e-23193.91prostaglandin E synthase 2-like [Cucurbita pepo subsp. pepo][more]
KAG6585529.15.27e-22893.04Prostaglandin E synthase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023002430.12.89e-22592.17prostaglandin E synthase 2-like [Cucurbita maxima][more]
XP_022951333.11.94e-22291.88prostaglandin E synthase 2-like [Cucurbita moschata][more]
XP_022144146.14.61e-18879.71prostaglandin E synthase 2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1KNY11.40e-22592.17Microsomal prostaglandin E synthase 2 OS=Cucurbita maxima OX=3661 GN=LOC11149627... [more]
A0A6J1GHF39.37e-22391.88Microsomal prostaglandin E synthase 2 OS=Cucurbita moschata OX=3662 GN=LOC111454... [more]
A0A6J1CSF92.23e-18879.71Microsomal prostaglandin E synthase 2 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A5A7VH912.86e-18279.01Microsomal prostaglandin E synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BDA92.86e-18279.01Microsomal prostaglandin E synthase 2 OS=Cucumis melo OX=3656 GN=LOC103488396 PE... [more]
Match NameE-valueIdentityDescription
AT5G42150.18.6e-10458.55Glutathione S-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 90..166
e-value: 7.2E-28
score: 98.2
coord: 262..340
e-value: 1.2E-30
score: 107.0
NoneNo IPR availableGENE3D1.20.1050.10coord: 344..514
e-value: 2.5E-58
score: 198.0
NoneNo IPR availablePANTHERPTHR12782:SF11GLUTATHIONE S-TRANSFERASE FAMILY PROTEINcoord: 459..514
coord: 1..158
coord: 173..447
NoneNo IPR availablePANTHERPTHR12782MICROSOMAL PROSTAGLANDIN E SYNTHASE-2coord: 459..514
coord: 173..447
coord: 1..158
NoneNo IPR availablePROSITEPS51354GLUTAREDOXIN_2coord: 85..180
score: 12.744536
NoneNo IPR availablePROSITEPS51354GLUTAREDOXIN_2coord: 257..354
score: 13.782504
IPR002109GlutaredoxinPFAMPF00462Glutaredoxincoord: 97..151
e-value: 2.1E-11
score: 43.8
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 271..338
e-value: 3.3E-13
score: 49.8
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 267..343
score: 10.315787
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 259..444
e-value: 0.0
score: 309.6
IPR034334Prostaglandin E synthase 2SFLDSFLDG01203Prostaglandin_E_synthase_likecoord: 259..444
e-value: 0.0
score: 309.6
IPR011767Glutaredoxin active sitePROSITEPS00195GLUTAREDOXIN_1coord: 271..287
IPR011767Glutaredoxin active sitePROSITEPS00195GLUTAREDOXIN_1coord: 99..115
IPR034335Prostaglandin E synthase 2, C-terminalCDDcd03197GST_C_mPGES2coord: 348..510
e-value: 4.24979E-70
score: 219.015
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 96..157
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 268..362
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 337..508

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g03540.1Cp4.1LG07g03540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0001516 prostaglandin biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0036134 12-hydroxyheptadecatrienoic acid synthase activity
molecular_function GO:0004362 glutathione-disulfide reductase (NADPH) activity
molecular_function GO:0097573 glutathione oxidoreductase activity
molecular_function GO:0050220 prostaglandin-E synthase activity
molecular_function GO:0005515 protein binding