Cp4.1LG07g01640 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g01640
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCarbonic anhydrase
LocationCp4.1LG07: 986064 .. 989019 (-)
RNA-Seq ExpressionCp4.1LG07g01640
SyntenyCp4.1LG07g01640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATTTGGTTCTTCCATCTCTCTCTCTCTCAATCATGGGATTTTTGGGGATTTTCTCTTTGAAATGTTAGAAAATTAGGAAAGTGGCCAAACGCAAACACGATATGATCTCTATGTCATCTCCAACGAAGTGGGGATCGATGTGGATAAGCTTGAAACGAGACGTTCATCTTTACAATTTCGCGTGTATAAATGGAACAGAAATTCAGCTGTAAGAAACTTTTCAATACACTCGATCATTTCAGTTGGGAAAGGCGGGAACCCAAATGGGTCGTTTAATTCGTAAGTTTTCTATACATTTTTCTTCATCTAATTGCTTGATTTCGTGCCTGTGTGCTTTTTGGGTACGTTTCAGATTCAATTTCTTTGTGAAATTATCGTTGCTGATAGGTCCAAGAGCTTGCTGGAATTGGTTATTTTCGGAAATGGCTGCAATTAGACCGACCTTAATCCACAAGAATCCTTTCCTTGCTTCATCATTTTCAGTCGGGACAAACTCTCTTTCTGTCAGTTCTTGTATCTCTCTGTTTTTCGTTTCTAATTCCTTGTTAATTCCAGTTTTAAATAGGTTTCTTAATGGACTTTGAATTTGAATCGACAGATGAAGGAAGAAATCGACGAAAACCATCTGGAAGTGCCATCTAATCGCAATCACGAGTGGGTTCTTTCTGCTAATTTACTTTATGAATCTGTTAATGGAAACTCCCATGAATTTTCTCTTCTGGTTCTTGATTTTTTTTTCTCTTGATTACAAGGCTGGAGGTCTCAAGAAACTCATTAGGGCTTGCCCAAGAACGATCATCAAGGATGGGAGTGAAAAATGTGGCTCAAGTAAAAGATGCAGATAAGGTGTTTGATGAAATGAAAATGAGATTTCTGAGTTTTAAGAAGCACAAGTACTTGTAAGTAGCAGTGGATACACGATTAGCTAATCTTTGGTTCTGTTAGGAAAGATCAAAATCAAATTGTGTTTGGTTTTTAATCCAATGATTTCATTGAAACAGGAAAAATTTAGAGCATTTTCAAGCTCTGGCAGAGCTACAAGCCCCTAAGGTAGTTAAGATGATGAACAAGTCCCTGTGATTTCTCTTTGATTCTTGTGTCCTGTTTGTACTATCTCTGCTCATTATCTTGAATATTTCAACACAAGCTCACAAGTAGAACAGTTCATGGTGATTTCTTGTGTCGATTCTAGAGTATGCCCATCTAACATTCTTGGATTTCAACCCGGAGAAGCTTTCACTGTTCGCAACGTAGCGAACATCGTTCCGTCATGGGAGGTATATATATAGTCTAGTGGGAATGTCTTGTATTATCTAAGTCAGACAGGCAAATAACTAACTTCAAGTTCTTATGCAGAATGGACCAACTGAAACCAATGCAGCCCTTGAATTTGCAGTAAATACACTTAAAGTGGGTTGTTTTCCGTTCTTCATTCAGTTAAATTTGATCTTTATAATCTCTAGACTTCATATTAAGAGAGCTATTTGTATACTGAAGGTTGAGAACATTTTAGTCATAGGCCACAGTAGCTGTGCTGGAATTCAGAGCCTTATGAGCATGGAAGATGATGCTACCGACTCGAGGTTTCACTTTCTCGAACTCCATCATGTCTTCTTAATCTGTTTTTCTCGATTGTTCTCGTTTTCGATCTGTAATTTATAGCTTATTCTGCTATGGTAGCTTTGTTCATAAATGGATGGTGAATGCAAAAGTTGCTAAATTAAGAGCAAAAGCTGCTGCTGCACATCTTAATTTTGACCAGCAATGTAAACATTGTGAGAAGGTATTGATCTTCTTGTTGCATGAACTTTTAGATTAGAATCCATTTCTTTATTAGTTCGTATTCATAATTCCTTACTCTGTTACCCCGTTGCGTTAGTCCACAAGCTAAGACTCTATGACTGATGATGCTATATTCTCGGGCCATGGTAAAAGGCTCGAAGGGAATGAGTTCATGTCATGATAACTAATTGCTTAGAATGTTATTCTACGAGTTTCCTCAGCAAGCGAGTGTAGTGGGGTTGATATGGTATTTAGTGCAAATAGTCGAGGTGCACACAAGTTAACTCAAAAGATTACCAATCTCGGTTCCCCTTTTGTCACCGAAGAATCAATTTCCTTATTAGTTCGTAAGCATAATTCCTTACTCTATTACCCTCTTGAGTTAGTCCAAAAGATTTATATTGATTTGACAAGCTTAGACTATATGCTTGATGTTATATTCTCGGGCCATAGTAAAAGGCTCGAAGGGAGTGAGTTCATGTCATGAAGACTCGTTGCCTAGAATATTATTCTACGAGTTTCCTCGGAAAGTGAGCATAGTGGGGCTGATCCGTTATCTGGTGCAAATAGTCGAGGTGCATACAAGTTAACTCAAAAGATTACTAATCTCGGTTCCCGTTTTGCCGCCAAGAATCAATCAACTTTTAGATTACAATCCATTTCTTTACTAGTTCGTATGCATAATTCCTTACTCTGTTATCCTGTTGAGTTAGTCCAAAAGCTTTATATTTCTTTTGACAAGCTAAGTCTACATGACCAATGCTATATTCTCGAGTCATAGTAAAAGGCTCGAAGGGAATGAGTTCATGTCATGATGACTCATTGCCTAGGATATTATTCTACGAGTTTCCTCAGCAAGCGAGTGTAGTAGGGTTGATCTGTTATTCGGTGCATATAGTCGAGACGCACACGAGTTAACTCAAAAGATTACTAATCTCAACGGTTTTCCTTTTTGCCACCAGGAATCGATCAACCTTTCATTGAAGAACTTAATGACGTATCCATGGATCGAAGAGCGGTTAAAACAGGAATCGCTTGCTGTTCATGGAGGATACTATGATTTCTTGAGTTGTACATTTGAGAAGTGGACTCTTGATTACAAGAACACCTGCAAAGCTGCTGGTGATGATGATCATGGATTTCAGATCAAAAATCAATCAGTATGGTGTTGA

mRNA sequence

TAATTTGGTTCTTCCATCTCTCTCTCTCTCAATCATGGGATTTTTGGGGATTTTCTCTTTGAAATGTTAGAAAATTAGGAAAGTGGCCAAACGCAAACACGATATGATCTCTATGTCATCTCCAACGAAGTGGGGATCGATGTGGATAAGCTTGAAACGAGACGTTCATCTTTACAATTTCGCGTGTATAAATGGAACAGAAATTCAGCTGTAAGAAACTTTTCAATACACTCGATCATTTCAGTTGGGAAAGGCGGGAACCCAAATGGGTCGTTTAATTCGTCCAAGAGCTTGCTGGAATTGGTTATTTTCGGAAATGGCTGCAATTAGACCGACCTTAATCCACAAGAATCCTTTCCTTGCTTCATCATTTTCAGTCGGGACAAACTCTCTTTCTATGAAGGAAGAAATCGACGAAAACCATCTGGAAGTGCCATCTAATCGCAATCACGAGCTGGAGGTCTCAAGAAACTCATTAGGGCTTGCCCAAGAACGATCATCAAGGATGGGAGTGAAAAATGTGGCTCAAGTAAAAGATGCAGATAAGGTGTTTGATGAAATGAAAATGAGATTTCTGAGTTTTAAGAAGCACAAGTACTTGAAAAATTTAGAGCATTTTCAAGCTCTGGCAGAGCTACAAGCCCCTAAGGTAGTTAAGATGATGAACAAGTCCCTCTCACAAGTAGAACAGTTCATGGTGATTTCTTGTGTCGATTCTAGAGTATGCCCATCTAACATTCTTGGATTTCAACCCGGAGAAGCTTTCACTGTTCGCAACGTAGCGAACATCGTTCCGTCATGGGAGAATGGACCAACTGAAACCAATGCAGCCCTTGAATTTGCAGTAAATACACTTAAAGTTGAGAACATTTTAGTCATAGGCCACAGTAGCTGTGCTGGAATTCAGAGCCTTATGAGCATGGAAGATGATGCTACCGACTCGAGCTTATTCTGCTATGGTAGCTTTGTTCATAAATGGATGGTGAATGCAAAAGTTGCTAAATTAAGAGCAAAAGCTGCTGCTGCACATCTTAATTTTGACCAGCAATGTAAACATTGTGAGAAGAACTTAATGACGTATCCATGGATCGAAGAGCGGTTAAAACAGGAATCGCTTGCTGTTCATGGAGGATACTATGATTTCTTGAGTTGTACATTTGAGAAGTGGACTCTTGATTACAAGAACACCTGCAAAGCTGCTGGTGATGATGATCATGGATTTCAGATCAAAAATCAATCAGTATGGTGTTGA

Coding sequence (CDS)

ATGGGTCGTTTAATTCGTCCAAGAGCTTGCTGGAATTGGTTATTTTCGGAAATGGCTGCAATTAGACCGACCTTAATCCACAAGAATCCTTTCCTTGCTTCATCATTTTCAGTCGGGACAAACTCTCTTTCTATGAAGGAAGAAATCGACGAAAACCATCTGGAAGTGCCATCTAATCGCAATCACGAGCTGGAGGTCTCAAGAAACTCATTAGGGCTTGCCCAAGAACGATCATCAAGGATGGGAGTGAAAAATGTGGCTCAAGTAAAAGATGCAGATAAGGTGTTTGATGAAATGAAAATGAGATTTCTGAGTTTTAAGAAGCACAAGTACTTGAAAAATTTAGAGCATTTTCAAGCTCTGGCAGAGCTACAAGCCCCTAAGGTAGTTAAGATGATGAACAAGTCCCTCTCACAAGTAGAACAGTTCATGGTGATTTCTTGTGTCGATTCTAGAGTATGCCCATCTAACATTCTTGGATTTCAACCCGGAGAAGCTTTCACTGTTCGCAACGTAGCGAACATCGTTCCGTCATGGGAGAATGGACCAACTGAAACCAATGCAGCCCTTGAATTTGCAGTAAATACACTTAAAGTTGAGAACATTTTAGTCATAGGCCACAGTAGCTGTGCTGGAATTCAGAGCCTTATGAGCATGGAAGATGATGCTACCGACTCGAGCTTATTCTGCTATGGTAGCTTTGTTCATAAATGGATGGTGAATGCAAAAGTTGCTAAATTAAGAGCAAAAGCTGCTGCTGCACATCTTAATTTTGACCAGCAATGTAAACATTGTGAGAAGAACTTAATGACGTATCCATGGATCGAAGAGCGGTTAAAACAGGAATCGCTTGCTGTTCATGGAGGATACTATGATTTCTTGAGTTGTACATTTGAGAAGTGGACTCTTGATTACAAGAACACCTGCAAAGCTGCTGGTGATGATGATCATGGATTTCAGATCAAAAATCAATCAGTATGGTGTTGA

Protein sequence

MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEKNLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Homology
BLAST of Cp4.1LG07g01640 vs. ExPASy Swiss-Prot
Match: Q94CE3 (Beta carbonic anhydrase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA5 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 7.8e-71
Identity = 143/275 (52.00%), Postives = 188/275 (68.36%), Query Frame = 0

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK KY+ + EH++ 
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYMDDFEHYKN 111

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LA+ QAPK              F+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E
Sbjct: 112 LADAQAPK--------------FLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYE 171

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           +GPTET AALEF+VNTL VENILVIGHS C GIQ+LM MED+  DS      SF+H W+V
Sbjct: 172 SGPTETKAALEFSVNTLNVENILVIGHSRCGGIQALMKMEDEG-DSR-----SFIHNWVV 231

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
             K AK   KA A++L+FD QC+HCEK         L+ YPWIEE+++Q SL++HGGYY+
Sbjct: 232 VGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIEEKVRQGSLSLHGGYYN 291

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 328
           F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 FVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 300

BLAST of Cp4.1LG07g01640 vs. ExPASy Swiss-Prot
Match: Q9C6F5 (Beta carbonic anhydrase 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCA6 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-60
Identity = 130/315 (41.27%), Postives = 183/315 (58.10%), Query Frame = 0

Query: 21  IRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      L   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAELQAPKVVKMMNKSLSQV 140
                    + +    DEM+ RFL FK+ KYL  +E F+ALA  Q+PKV           
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKV----------- 132

Query: 141 EQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNTLKVE 200
              MVI C DSRVCPS +LGFQPGEAFT+RNVAN+V   +NGPTETN+ALEFAV TL+VE
Sbjct: 133 ---MVIGCADSRVCPSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTTLQVE 192

Query: 201 NILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQ 260
           NI+V+GHS+C GI +LMS ++     S     S V +W++N K AKLR + A++HL+FD+
Sbjct: 193 NIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAAKLRTQLASSHLSFDE 252

Query: 261 QCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAA 320
           QC++CEK        NL+TY WI +R+K+  + +HG YY+   C+ EKW L    T    
Sbjct: 253 QCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCSLEKWRLSSDKT---- 289

Query: 321 GDDDHGFQIKNQSVW 328
              ++GF I ++ +W
Sbjct: 313 ---NYGFYISDREIW 289

BLAST of Cp4.1LG07g01640 vs. ExPASy Swiss-Prot
Match: P17067 (Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 114/308 (37.01%), Postives = 165/308 (53.57%), Query Frame = 0

Query: 9   ACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSL--SMKEEIDENHLEVPSNRNHELEV 68
           A  N   S  ++  P+LI   P  ASS  + T  L   M +  DE   E+      + E+
Sbjct: 32  ASLNTSSSSSSSTFPSLIQDKPVFASSSPIITPVLREEMGKGYDEAIEELQKLLREKTEL 91

Query: 69  SRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAELQA 128
              +    ++ ++++G  + +      +  + +K  FL FKK KY KN   +  LA+ Q+
Sbjct: 92  KATAAEKVEQITAQLGTTSSSDGIPKSEASERIKTGFLHFKKEKYDKNPALYGELAKGQS 151

Query: 129 PKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGP-TE 188
           P               FMV +C DSRVCPS++L FQPGEAF VRNVAN+VP ++      
Sbjct: 152 P--------------PFMVFACSDSRVCPSHVLDFQPGEAFVVRNVANLVPPYDQAKYAG 211

Query: 189 TNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVA 248
           T AA+E+AV  LKV NI+VIGHS+C GI+ L+S   D T S+      F+ +W+     A
Sbjct: 212 TGAAIEYAVLHLKVSNIVVIGHSACGGIKGLLSFPFDGTYST-----DFIEEWVKIGLPA 271

Query: 249 KLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYDFLSCT 306
           K + KA      F + C HCEK        NL+TYP++ E L  ++LA+ GGYYDF+  +
Sbjct: 272 KAKVKAQHGDAPFAELCTHCEKEAVNASLGNLLTYPFVREGLVNKTLALKGGYYDFVKGS 320

BLAST of Cp4.1LG07g01640 vs. ExPASy Swiss-Prot
Match: Q94CE4 (Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-43
Identity = 99/235 (42.13%), Postives = 137/235 (58.30%), Query Frame = 0

Query: 87  AQVKDADK----VFDEMKMRFLSFKKHKYLKNLEHFQALAELQAPKVVKMMNKSLSQVEQ 146
           A++K+ D       + +K  F  FK  KYLKN   F  LA+ Q PK              
Sbjct: 58  AELKELDSSNSDAIERIKTGFTQFKTEKYLKNSTLFNHLAKTQTPK-------------- 117

Query: 147 FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSW-ENGPTETNAALEFAVNTLKVEN 206
           F+V +C DSRVCPS+IL FQPGEAF VRN+AN+VP + +   +   AA+E+AV  LKVEN
Sbjct: 118 FLVFACSDSRVCPSHILNFQPGEAFVVRNIANMVPPFDQKRHSGVGAAVEYAVVHLKVEN 177

Query: 207 ILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQ 266
           ILVIGHS C GI+ LMS+EDDA  +       F+  W+     A+ + K     L++D Q
Sbjct: 178 ILVIGHSCCGGIKGLMSIEDDAAPTQ----SDFIENWVKIGASARNKIKEEHKDLSYDDQ 237

Query: 267 CKHCEK--------NLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNT 309
           C  CEK        NL++YP++   + + +LA+ GG+Y+F+  TF+ W LD+K T
Sbjct: 238 CNKCEKEAVNVSLGNLLSYPFVRAEVVKNTLAIRGGHYNFVKGTFDLWELDFKTT 274

BLAST of Cp4.1LG07g01640 vs. ExPASy Swiss-Prot
Match: P16016 (Carbonic anhydrase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-41
Identity = 108/310 (34.84%), Postives = 162/310 (52.26%), Query Frame = 0

Query: 5   IRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHEL 64
           +RP    N   +  +++ P+LI   P  A+   + T +L      +E    +    + + 
Sbjct: 24  LRPTFIANSRVNPSSSVPPSLIRNQPVFAAPAPIITPTLKEDMAYEEAIAALKKLLSEKG 83

Query: 65  EVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAEL 124
           E+   +     + +S +          A      +K  F+ FKK KY KN   +  L++ 
Sbjct: 84  ELENEAASKVAQITSELA---DGGTPSASYPVQRIKEGFIKFKKEKYEKNPALYGELSKG 143

Query: 125 QAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGP- 184
           QAPK              FMV +C DSRVCPS++L FQPGEAF VRN+AN+VP ++    
Sbjct: 144 QAPK--------------FMVFACSDSRVCPSHVLDFQPGEAFMVRNIANMVPVFDKDKY 203

Query: 185 TETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAK 244
               AA+E+AV  LKVENI+VIGHS+C GI+ LMS  D    ++      F+  W+    
Sbjct: 204 AGVGAAIEYAVLHLKVENIVVIGHSACGGIKGLMSFPDAGPTTT-----DFIEDWVKICL 263

Query: 245 VAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYDFLS 304
            AK +  A   +  F +QC HCEK        NL+TYP++ + L +++LA+ GGYYDF++
Sbjct: 264 PAKHKVLAEHGNATFAEQCTHCEKEAVNVSLGNLLTYPFVRDGLVKKTLALQGGYYDFVN 311

Query: 305 CTFEKWTLDY 306
            +FE W L+Y
Sbjct: 324 GSFELWGLEY 311

BLAST of Cp4.1LG07g01640 vs. NCBI nr
Match: XP_023537827.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627 bits (1618), Expect = 2.29e-226
Identity = 314/336 (93.45%), Postives = 314/336 (93.45%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR
Sbjct: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 121 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV
Sbjct: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 300

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

BLAST of Cp4.1LG07g01640 vs. NCBI nr
Match: XP_022951348.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 621 bits (1601), Expect = 8.94e-224
Identity = 311/336 (92.56%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           MGRLIRPRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENHLEV SNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 121 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV
Sbjct: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 300

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

BLAST of Cp4.1LG07g01640 vs. NCBI nr
Match: XP_023537825.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619 bits (1595), Expect = 3.49e-222
Identity = 310/336 (92.26%), Postives = 311/336 (92.56%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           +  LI PRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR
Sbjct: 43  LSLLIGPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 102

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 103 NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 162

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 163 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 222

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV
Sbjct: 223 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 282

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 283 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 342

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 343 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

BLAST of Cp4.1LG07g01640 vs. NCBI nr
Match: XP_023001870.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 611 bits (1575), Expect = 8.21e-220
Identity = 305/336 (90.77%), Postives = 310/336 (92.26%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           MGRLIRPRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENH+EVPSNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
            HELEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKHKYL+NLEHFQA
Sbjct: 61  YHELEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQA 120

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 121 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWM 
Sbjct: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMA 240

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 300

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 301 FLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 322

BLAST of Cp4.1LG07g01640 vs. NCBI nr
Match: XP_022951346.1 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 612 bits (1578), Expect = 1.36e-219
Identity = 307/336 (91.37%), Postives = 309/336 (91.96%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           +  LI PRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENHLEV SNR
Sbjct: 43  LSLLIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 102

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 103 NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 162

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 163 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 222

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV
Sbjct: 223 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 282

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 283 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 342

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 343 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

BLAST of Cp4.1LG07g01640 vs. ExPASy TrEMBL
Match: A0A6J1GHG7 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=3 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 4.33e-224
Identity = 311/336 (92.56%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           MGRLIRPRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENHLEV SNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 121 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV
Sbjct: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 300

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 322

BLAST of Cp4.1LG07g01640 vs. ExPASy TrEMBL
Match: A0A6J1KMD2 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111495926 PE=3 SV=1)

HSP 1 Score: 611 bits (1575), Expect = 3.97e-220
Identity = 305/336 (90.77%), Postives = 310/336 (92.26%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           MGRLIRPRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENH+EVPSNR
Sbjct: 1   MGRLIRPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNR 60

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
            HELEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKHKYL+NLEHFQA
Sbjct: 61  YHELEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQA 120

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 121 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWM 
Sbjct: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMA 240

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 300

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 301 FLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 322

BLAST of Cp4.1LG07g01640 vs. ExPASy TrEMBL
Match: A0A6J1GHC0 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=4 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 6.58e-220
Identity = 307/336 (91.37%), Postives = 309/336 (91.96%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           +  LI PRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENHLEV SNR
Sbjct: 43  LSLLIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 102

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 103 NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 162

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 163 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 222

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV
Sbjct: 223 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 282

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 283 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 342

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 343 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 364

BLAST of Cp4.1LG07g01640 vs. ExPASy TrEMBL
Match: A0A6J1KHU8 (beta carbonic anhydrase 5, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495926 PE=4 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 3.87e-216
Identity = 301/336 (89.58%), Postives = 307/336 (91.37%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           +  LI PRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENH+EVPSNR
Sbjct: 31  LSLLIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHMEVPSNR 90

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
            HELEVSRNSLGLAQERSS MGVKNVAQVKDADKVFDEMKMRFLSFKKHKYL+NLEHFQA
Sbjct: 91  YHELEVSRNSLGLAQERSSWMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLENLEHFQA 150

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 151 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 210

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWM 
Sbjct: 211 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMA 270

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 271 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 330

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAG+DDHGFQIKNQS+WC
Sbjct: 331 FLSCTFEKWTLDYKNTCKAAGNDDHGFQIKNQSLWC 352

BLAST of Cp4.1LG07g01640 vs. ExPASy TrEMBL
Match: A0A6J1GHD3 (beta carbonic anhydrase 5, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=4 SV=1)

HSP 1 Score: 592 bits (1525), Expect = 6.26e-212
Identity = 301/336 (89.58%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MGRLIRPRACWNWLFSEMAAIRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNR 60
           +  LI PRACW+WLFSEMAAIRPTLIHKNPFL SSFSVGTNSLSMKEEIDENHLEV SNR
Sbjct: 43  LSLLIGPRACWSWLFSEMAAIRPTLIHKNPFLVSSFSVGTNSLSMKEEIDENHLEVTSNR 102

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA
Sbjct: 103 NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 162

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LAELQAPK              FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE
Sbjct: 163 LAELQAPK--------------FMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 222

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSS      FVHKWMV
Sbjct: 223 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSS------FVHKWMV 282

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
           NAKVAKLRAKAAAAHLNFDQQCKHCEK        NLMTYPWIEERLKQESLAVHGGYYD
Sbjct: 283 NAKVAKLRAKAAAAHLNFDQQCKHCEKESINLSLKNLMTYPWIEERLKQESLAVHGGYYD 342

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 328
           FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC
Sbjct: 343 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVWC 358

BLAST of Cp4.1LG07g01640 vs. TAIR 10
Match: AT4G33580.1 (beta carbonic anhydrase 5 )

HSP 1 Score: 268.9 bits (686), Expect = 5.5e-72
Identity = 143/275 (52.00%), Postives = 188/275 (68.36%), Query Frame = 0

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQA 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK KY+ + EH++ 
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYMDDFEHYKN 111

Query: 121 LAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWE 180
           LA+ QAPK              F+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +E
Sbjct: 112 LADAQAPK--------------FLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPYE 171

Query: 181 NGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMV 240
           +GPTET AALEF+VNTL VENILVIGHS C GIQ+LM MED+  DS      SF+H W+V
Sbjct: 172 SGPTETKAALEFSVNTLNVENILVIGHSRCGGIQALMKMEDEG-DSR-----SFIHNWVV 231

Query: 241 NAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYD 300
             K AK   KA A++L+FD QC+HCEK         L+ YPWIEE+++Q SL++HGGYY+
Sbjct: 232 VGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIEEKVRQGSLSLHGGYYN 291

Query: 301 FLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 328
           F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 FVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 300

BLAST of Cp4.1LG07g01640 vs. TAIR 10
Match: AT4G33580.2 (beta carbonic anhydrase 5 )

HSP 1 Score: 265.4 bits (677), Expect = 6.1e-71
Identity = 143/276 (51.81%), Postives = 189/276 (68.48%), Query Frame = 0

Query: 61  NHELEVSRNSLGLAQERSSRMGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLK-NLEHFQ 120
           N ++  S  + GL QE +   GV  + +  + D VFD+MK RFL+FKK KY++ + EH++
Sbjct: 52  NLQVMASGKTPGLTQEAN---GVA-IDRQNNTD-VFDDMKQRFLAFKKLKYIRDDFEHYK 111

Query: 121 ALAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSW 180
            LA+ QAPK              F+VI+C DSRVCPS +LGFQPG+AFTVRN+AN+VP +
Sbjct: 112 NLADAQAPK--------------FLVIACADSRVCPSAVLGFQPGDAFTVRNIANLVPPY 171

Query: 181 ENGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWM 240
           E+GPTET AALEF+VNTL VENILVIGHS C GIQ+LM MED+  DS      SF+H W+
Sbjct: 172 ESGPTETKAALEFSVNTLNVENILVIGHSRCGGIQALMKMEDEG-DSR-----SFIHNWV 231

Query: 241 VNAKVAKLRAKAAAAHLNFDQQCKHCEK--------NLMTYPWIEERLKQESLAVHGGYY 300
           V  K AK   KA A++L+FD QC+HCEK         L+ YPWIEE+++Q SL++HGGYY
Sbjct: 232 VVGKKAKESTKAVASNLHFDHQCQHCEKASINHSLERLLGYPWIEEKVRQGSLSLHGGYY 291

Query: 301 DFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 328
           +F+ CTFEKWT+DY    +    +  G  +K++SVW
Sbjct: 292 NFVDCTFEKWTVDYA-ASRGKKKEGSGIAVKDRSVW 301

BLAST of Cp4.1LG07g01640 vs. TAIR 10
Match: AT1G58180.2 (beta carbonic anhydrase 6 )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 130/315 (41.27%), Postives = 183/315 (58.10%), Query Frame = 0

Query: 21  IRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      L   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAELQAPKVVKMMNKSLSQV 140
                    + +    DEM+ RFL FK+ KYL  +E F+ALA  Q+PKV           
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKV----------- 132

Query: 141 EQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNTLKVE 200
              MVI C DSRVCPS +LGFQPGEAFT+RNVAN+V   +NGPTETN+ALEFAV TL+VE
Sbjct: 133 ---MVIGCADSRVCPSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTTLQVE 192

Query: 201 NILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQ 260
           NI+V+GHS+C GI +LMS ++     S     S V +W++N K AKLR + A++HL+FD+
Sbjct: 193 NIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAAKLRTQLASSHLSFDE 252

Query: 261 QCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAA 320
           QC++CEK        NL+TY WI +R+K+  + +HG YY+   C+ EKW L    T    
Sbjct: 253 QCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCSLEKWRLSSDKT---- 289

Query: 321 GDDDHGFQIKNQSVW 328
              ++GF I ++ +W
Sbjct: 313 ---NYGFYISDREIW 289

BLAST of Cp4.1LG07g01640 vs. TAIR 10
Match: AT1G58180.4 (beta carbonic anhydrase 6 )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 130/315 (41.27%), Postives = 183/315 (58.10%), Query Frame = 0

Query: 21  IRPTLIHKNPFLASSFSVGTNSLSMKEEIDENHLEVPSNRNHELEVSRNSLGLAQERSSR 80
           +  T +H+N  L     +G++   + E      L   +N   EL +    + L +E    
Sbjct: 13  VSATSVHQNGCLHKLQQIGSDRFQLGEAKAIRLLPRRTNMVQELGIREEFMDLNRE---- 72

Query: 81  MGVKNVAQVKDADKVFDEMKMRFLSFKKHKYLKNLEHFQALAELQAPKVVKMMNKSLSQV 140
                    + +    DEM+ RFL FK+ KYL  +E F+ALA  Q+PKV           
Sbjct: 73  --------TETSYDFLDEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKV----------- 132

Query: 141 EQFMVISCVDSRVCPSNILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNTLKVE 200
              MVI C DSRVCPS +LGFQPGEAFT+RNVAN+V   +NGPTETN+ALEFAV TL+VE
Sbjct: 133 ---MVIGCADSRVCPSYVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTTLQVE 192

Query: 201 NILVIGHSSCAGIQSLMSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQ 260
           NI+V+GHS+C GI +LMS ++     S     S V +W++N K AKLR + A++HL+FD+
Sbjct: 193 NIIVMGHSNCGGIAALMSHQNHQGQHS-----SLVERWVMNGKAAKLRTQLASSHLSFDE 252

Query: 261 QCKHCEK--------NLMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAA 320
           QC++CEK        NL+TY WI +R+K+  + +HG YY+   C+ EKW L    T    
Sbjct: 253 QCRNCEKESIKDSVMNLITYSWIRDRVKRGEVKIHGCYYNLSDCSLEKWRLSSDKT---- 289

Query: 321 GDDDHGFQIKNQSVW 328
              ++GF I ++ +W
Sbjct: 313 ---NYGFYISDREIW 289

BLAST of Cp4.1LG07g01640 vs. TAIR 10
Match: AT1G58180.3 (beta carbonic anhydrase 6 )

HSP 1 Score: 233.0 bits (593), Expect = 3.4e-61
Identity = 118/239 (49.37%), Postives = 158/239 (66.11%), Query Frame = 0

Query: 97  DEMKMRFLSFKKHKYLKNLEHFQALAELQAPKVVKMMNKSLSQVEQFMVISCVDSRVCPS 156
           DEM+ RFL FK+ KYL  +E F+ALA  Q+PKV              MVI C DSRVCPS
Sbjct: 26  DEMRHRFLKFKRQKYLPEIEKFKALAIAQSPKV--------------MVIGCADSRVCPS 85

Query: 157 NILGFQPGEAFTVRNVANIVPSWENGPTETNAALEFAVNTLKVENILVIGHSSCAGIQSL 216
            +LGFQPGEAFT+RNVAN+V   +NGPTETN+ALEFAV TL+VENI+V+GHS+C GI +L
Sbjct: 86  YVLGFQPGEAFTIRNVANLVTPVQNGPTETNSALEFAVTTLQVENIIVMGHSNCGGIAAL 145

Query: 217 MSMEDDATDSSLFCYGSFVHKWMVNAKVAKLRAKAAAAHLNFDQQCKHCEK--------N 276
           MS ++     S     S V +W++N K AKLR + A++HL+FD+QC++CEK        N
Sbjct: 146 MSHQNHQGQHS-----SLVERWVMNGKAAKLRTQLASSHLSFDEQCRNCEKESIKDSVMN 205

Query: 277 LMTYPWIEERLKQESLAVHGGYYDFLSCTFEKWTLDYKNTCKAAGDDDHGFQIKNQSVW 328
           L+TY WI +R+K+  + +HG YY+   C+ EKW L    T       ++GF I ++ +W
Sbjct: 206 LITYSWIRDRVKRGEVKIHGCYYNLSDCSLEKWRLSSDKT-------NYGFYISDREIW 238

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94CE37.8e-7152.00Beta carbonic anhydrase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BCA5... [more]
Q9C6F53.6e-6041.27Beta carbonic anhydrase 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCA6... [more]
P170671.1e-4337.01Carbonic anhydrase, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=1[more]
Q94CE41.4e-4342.13Beta carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=BCA4 PE=1 SV=1[more]
P160161.7e-4134.84Carbonic anhydrase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023537827.12.29e-22693.45beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita pepo subsp. ... [more]
XP_022951348.18.94e-22492.56beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita moschata][more]
XP_023537825.13.49e-22292.26beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita pepo subsp. ... [more]
XP_023001870.18.21e-22090.77beta carbonic anhydrase 5, chloroplastic-like isoform X3 [Cucurbita maxima][more]
XP_022951346.11.36e-21991.37beta carbonic anhydrase 5, chloroplastic-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1GHG74.33e-22492.56Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111454204 PE=3 SV=1[more]
A0A6J1KMD23.97e-22090.77Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111495926 PE=3 SV=1[more]
A0A6J1GHC06.58e-22091.37beta carbonic anhydrase 5, chloroplastic-like isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1KHU83.87e-21689.58beta carbonic anhydrase 5, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1GHD36.26e-21289.58beta carbonic anhydrase 5, chloroplastic-like isoform X2 OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
AT4G33580.15.5e-7252.00beta carbonic anhydrase 5 [more]
AT4G33580.26.1e-7151.81beta carbonic anhydrase 5 [more]
AT1G58180.22.6e-6141.27beta carbonic anhydrase 6 [more]
AT1G58180.42.6e-6141.27beta carbonic anhydrase 6 [more]
AT1G58180.33.4e-6149.37beta carbonic anhydrase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001765Carbonic anhydraseSMARTSM00947Pro_CA_2coord: 136..302
e-value: 6.1E-33
score: 125.4
IPR001765Carbonic anhydrasePFAMPF00484Pro_CAcoord: 144..293
e-value: 1.4E-33
score: 116.4
IPR036874Carbonic anhydrase superfamilyGENE3D3.40.1050.10Carbonic anhydrasecoord: 85..311
e-value: 3.9E-52
score: 178.8
IPR036874Carbonic anhydrase superfamilySUPERFAMILY53056beta-carbonic anhydrase, cabcoord: 89..307
NoneNo IPR availablePANTHERPTHR11002:SF19CARBONIC ANHYDRASEcoord: 33..328
NoneNo IPR availablePANTHERPTHR11002CARBONIC ANHYDRASEcoord: 33..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g01640.1Cp4.1LG07g01640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015976 carbon utilization
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding