Cp4.1LG06g09420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g09420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG06: 9203312 .. 9204361 (-)
RNA-Seq ExpressionCp4.1LG06g09420
SyntenyCp4.1LG06g09420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGCCGTTCAGTGTTTCGTCGGAGATTTCCACCTCCCAACTTCCATGGAAGCACCGCCTTACTCTCCGATTCGCCGCCCTACTCTTCAACGCTTCTGTCCGATCAGATCTCACCGTTAATCGCCGGCTTCTCAATTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACAATCGACGCCTCTCGTGATCTCTTCCTCCGAATCTTCACCCCCACCGACGCCGCCATCAACGCCGCCGCCGTCGCCGCCACGGACTCACTCCTGCCGCCGGTAATTTTCTACTTTCACGGCGGCGGTTTCGCCTTCGGTTCTGCCGCCGCCACGTCAATGGACATGGCCGCCCGCCGTTTCAGTAAGGAACTCGGAGCTGTCATAATCTCCGTGAATTACCGTCTCGCTCCTCAGTTCCGATTCCCCTGCCAATACGACGACGGATTTGACGCCGTGAAATTCATCGACGAAATGGACGACGACGATCCATTGTTGGGTAAGGCAGATCTGAGCCGTTGCTTTATTTTGGGGGAAAGCGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCTTTCAAGAGAGTGAAAGTAATTGGGTTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATCAGCCTCCGTTAACTCTGAAACTTGCGGATTGGTTCTGGAAGGCGTTTTTGCCGGCGGGGGAAGACCGGGATCACGCGGCGGCGAATGTGAATGGACCGAACGGAAGGGATGTCTCCGGGCTGAGGAATTTTCCGGCGACGATTATTTTGGCCGGAGGATTGGATTTACTGATAGATCGGCAGAGGAGGTACTACGAGGAGCTGAAGAGAATGGGAAAGGAGGTGAAATTGGTGGTGTTTACGAACGGCGTCCATGGATTCTTTAGCTTTGAGGATCTGCCGGAATATTCTCTAATGATTAAGGAGATGAGGGATTTCATTGCAATGTTTCACCGGAATATTCTTTAA

mRNA sequence

ATGCAGCCGTTCAGTGTTTCGTCGGAGATTTCCACCTCCCAACTTCCATGGAAGCACCGCCTTACTCTCCGATTCGCCGCCCTACTCTTCAACGCTTCTGTCCGATCAGATCTCACCGTTAATCGCCGGCTTCTCAATTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACAATCGACGCCTCTCGTGATCTCTTCCTCCGAATCTTCACCCCCACCGACGCCGCCATCAACGCCGCCGCCGTCGCCGCCACGGACTCACTCCTGCCGCCGGTAATTTTCTACTTTCACGGCGGCGGTTTCGCCTTCGGTTCTGCCGCCGCCACGTCAATGGACATGGCCGCCCGCCGTTTCAGTAAGGAACTCGGAGCTGTCATAATCTCCGTGAATTACCGTCTCGCTCCTCAGTTCCGATTCCCCTGCCAATACGACGACGGATTTGACGCCGTGAAATTCATCGACGAAATGGACGACGACGATCCATTGTTGGGTAAGGCAGATCTGAGCCGTTGCTTTATTTTGGGGGAAAGCGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCTTTCAAGAGAGTGAAAGTAATTGGGTTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATCAGCCTCCGTTAACTCTGAAACTTGCGGATTGGTTCTGGAAGGCGTTTTTGCCGGCGGGGGAAGACCGGGATCACGCGGCGGCGAATGTGAATGGACCGAACGGAAGGGATGTCTCCGGGCTGAGGAATTTTCCGGCGACGATTATTTTGGCCGGAGGATTGGATTTACTGATAGATCGGCAGAGGAGGTACTACGAGGAGCTGAAGAGAATGGGAAAGGAGGTGAAATTGGTGGTGTTTACGAACGGCGTCCATGGATTCTTTAGCTTTGAGGATCTGCCGGAATATTCTCTAATGATTAAGGAGATGAGGGATTTCATTGCAATGTTTCACCGGAATATTCTTTAA

Coding sequence (CDS)

ATGCAGCCGTTCAGTGTTTCGTCGGAGATTTCCACCTCCCAACTTCCATGGAAGCACCGCCTTACTCTCCGATTCGCCGCCCTACTCTTCAACGCTTCTGTCCGATCAGATCTCACCGTTAATCGCCGGCTTCTCAATTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAATCCACGCCGTTTCCTCTTCCGATCTCACAATCGACGCCTCTCGTGATCTCTTCCTCCGAATCTTCACCCCCACCGACGCCGCCATCAACGCCGCCGCCGTCGCCGCCACGGACTCACTCCTGCCGCCGGTAATTTTCTACTTTCACGGCGGCGGTTTCGCCTTCGGTTCTGCCGCCGCCACGTCAATGGACATGGCCGCCCGCCGTTTCAGTAAGGAACTCGGAGCTGTCATAATCTCCGTGAATTACCGTCTCGCTCCTCAGTTCCGATTCCCCTGCCAATACGACGACGGATTTGACGCCGTGAAATTCATCGACGAAATGGACGACGACGATCCATTGTTGGGTAAGGCAGATCTGAGCCGTTGCTTTATTTTGGGGGAAAGCGCCGGGGGGAATCTAGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCTTTCAAGAGAGTGAAAGTAATTGGGTTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATCAGCCTCCGTTAACTCTGAAACTTGCGGATTGGTTCTGGAAGGCGTTTTTGCCGGCGGGGGAAGACCGGGATCACGCGGCGGCGAATGTGAATGGACCGAACGGAAGGGATGTCTCCGGGCTGAGGAATTTTCCGGCGACGATTATTTTGGCCGGAGGATTGGATTTACTGATAGATCGGCAGAGGAGGTACTACGAGGAGCTGAAGAGAATGGGAAAGGAGGTGAAATTGGTGGTGTTTACGAACGGCGTCCATGGATTCTTTAGCTTTGAGGATCTGCCGGAATATTCTCTAATGATTAAGGAGATGAGGGATTTCATTGCAATGTTTCACCGGAATATTCTTTAA

Protein sequence

MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL
Homology
BLAST of Cp4.1LG06g09420 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.5e-77
Identity = 153/330 (46.36%), Postives = 208/330 (63.03%), Query Frame = 0

Query: 15  LPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRD 74
           LP K R+ L   + + + + R D T+NRR L   D + PPNP  ++ VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGA 134
           L+ R++TP           + D +  PV+ +FHGGGFAF S  A   D   RRF+++L A
Sbjct: 73  LWFRLYTPH---------VSGDKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPA 132

Query: 135 VIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGH 194
            +ISVNYRLAP+ R+P QYDDGFDA+K+I+E +    L   ADLSRCF  G+SAGGN+ H
Sbjct: 133 YVISVNYRLAPEHRYPAQYDDGFDALKYIEE-NHGSILPANADLSRCFFAGDSAGGNIAH 192

Query: 195 HVAV---RASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFL 254
           +VA+   R    +F  VK+IG I+ QPFFGGEERTE+E +L   P ++    DW WKA  
Sbjct: 193 NVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM- 252

Query: 255 PAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKL 314
             G +RDH A NV GPN  D+SGL ++P T+++  G D L D QR YYE LK  GK+  L
Sbjct: 253 --GLNRDHEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATL 312

Query: 315 VVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 342
           + + N  H F+ F +LPE   +I  ++DF+
Sbjct: 313 IEYPNMFHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Cp4.1LG06g09420 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.6e-61
Identity = 130/318 (40.88%), Postives = 186/318 (58.49%), Query Frame = 0

Query: 28  LLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTDAAI 87
           L +N   R D T NR L  FLD K+P N N ++ V S D+ ID   +L  R++ P DA  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  NAAAV---AATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVNYRLA 147
           + +        D  + PVI +FHGG FA  SA +   D   RR     GAV++SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 207
           P+ R+PC YDDG+  +K++   +    L  K D   R F+ G+S+GGN+ H+VAVRA E 
Sbjct: 149 PENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE- 208

Query: 208 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 267
              R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GEDR+H A +
Sbjct: 209 --SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACS 268

Query: 268 VNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 327
             GP  + + GL +FP ++++  GLDL+ D Q +Y E LK+ G+EVKL+       GF+ 
Sbjct: 269 PFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYL 328

Query: 328 FEDLPEYSLMIKEMRDFI 342
             +   +  ++ E+  F+
Sbjct: 329 LPNNNHFHTVMDEIAAFV 339

BLAST of Cp4.1LG06g09420 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.5e-59
Identity = 138/326 (42.33%), Postives = 185/326 (56.75%), Query Frame = 0

Query: 28  LLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPT---D 87
           L +N   R+D T  R L  +LD ++P N   +  VSS D  ID S  L +RI+      D
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  AAINAAAVA------ATDSLLP---PVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAV 147
           A   AAAV        TD+      PVI +FHGG F   SA++T  D   RRF K    V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 IISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHH 207
           ++SVNYR AP+ R+PC YDDG+ A+K++  M       G    +R F+ G+S+GGN+ HH
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHH 208

Query: 208 VAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGE 267
           VAVRA++   + VKV G I     FGG ERTESE RL  +  +TL+  DW+WKA+LP   
Sbjct: 209 VAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDA 268

Query: 268 DRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFT 327
           DRDH A N  GPNGR + GL  F  ++I+  GLDL  DRQ  Y + L+  G  VK+V   
Sbjct: 269 DRDHPACNPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCE 328

Query: 328 NGVHGFFSFEDLPEYSLMIKEMRDFI 342
           N   GF+   +   Y  +++E+ DF+
Sbjct: 329 NATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Cp4.1LG06g09420 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 6.0e-53
Identity = 120/318 (37.74%), Postives = 173/318 (54.40%), Query Frame = 0

Query: 30  FNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTDAAINA 89
           +N   R D T NR L  +LD K+  N N +  V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  A-----AVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVNYRLA 149
                      D  + PVI +FHGG FA  SA +   D   RR       V++SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 209
           P+  +PC YDDG+ A+ +++       L  K D     F+ G+S+GGN+ H+VA+RA E 
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR---SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE- 210

Query: 210 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 269
               + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GEDR+H A N
Sbjct: 211 --SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACN 270

Query: 270 VNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 329
              P G+ + G+ +FP ++++  GLDL+ D Q  Y E LK+ G+EVKL+       GF+ 
Sbjct: 271 PFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYL 330

Query: 330 FEDLPEYSLMIKEMRDFI 342
             +   +  ++ E+  F+
Sbjct: 331 LPNNNHFHNVMDEISAFV 341

BLAST of Cp4.1LG06g09420 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.0e-52
Identity = 115/321 (35.83%), Postives = 179/321 (55.76%), Query Frame = 0

Query: 28  LLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTD--- 87
           L +    R D + NR L  FLD K+P N   +  V S D  +D++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ----AAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVN 147
                 +      +T  ++ PV+ +FHGG F   SA +   D   RR     G V++SV+
Sbjct: 89  QTRHGTLELTKPLSTTEIV-PVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVD 148

Query: 148 YRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAVRA 207
           YR +P+ R+PC YDDG++A+ ++          GK      ++ G+S+GGN+ H+VAVRA
Sbjct: 149 YRRSPEHRYPCAYDDGWNALNWVKSRVWLQS--GKDSNVYVYLAGDSSGGNIAHNVAVRA 208

Query: 208 SEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHA 267
           +    + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GEDRDH 
Sbjct: 209 TN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHP 268

Query: 268 AANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHG 327
           A N  GP G+ + G+ NFP ++++  GLDL+ D Q  Y + LK+ G EV L+       G
Sbjct: 269 ACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIG 328

Query: 328 FFSFEDLPEYSLMIKEMRDFI 342
           F+   +   +  +++E+  F+
Sbjct: 329 FYFLPNNDHFHCLMEELNKFV 341

BLAST of Cp4.1LG06g09420 vs. NCBI nr
Match: XP_023536262.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701 bits (1809), Expect = 1.13e-254
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120

Query: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR
Sbjct: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349
           ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349

BLAST of Cp4.1LG06g09420 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675 bits (1741), Expect = 1.94e-244
Identity = 336/349 (96.28%), Postives = 340/349 (97.42%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNAS+RSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTID SRDLFLRIFTPT        VAATDS+LPPVIFYFHGGGFAFGSAAATS
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT--------VAATDSVLPPVIFYFHGGGFAFGSAAATS 120

Query: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAV+ISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR
Sbjct: 121 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD+LIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349
           ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 341

BLAST of Cp4.1LG06g09420 vs. NCBI nr
Match: XP_022973499.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 671 bits (1731), Expect = 6.49e-243
Identity = 333/349 (95.42%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNAS+RSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTIDASRDLFLRIFTPT        VAATDSLLPPVIFYFHGGGFAFGSAAATS
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPT--------VAATDSLLPPVIFYFHGGGFAFGSAAATS 120

Query: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAV+ISVNYRL+PQFRFPCQYDDGFDAVKFIDEMDD+DPLLG+ADLSR
Sbjct: 121 MDMAARRFSKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD LIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349
           ELKRMGK+VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI MFHRNIL
Sbjct: 301 ELKRMGKDVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIGMFHRNIL 341

BLAST of Cp4.1LG06g09420 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 667 bits (1722), Expect = 1.53e-241
Identity = 334/349 (95.70%), Postives = 337/349 (96.56%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQP SVSSEISTSQLPWKHRLTLRFAALLFNAS+RSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTID SRDLFLRIFTPT        VAATDS+LPPVIFYFHGGGFAFGSA ATS
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT--------VAATDSVLPPVIFYFHGGGFAFGSADATS 120

Query: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAV+ISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR
Sbjct: 121 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349
           ELKRMGKEVKLVVFTNGVHGFFSFED PEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIAMFHRNIL 341

BLAST of Cp4.1LG06g09420 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 544 bits (1401), Expect = 1.14e-192
Identity = 270/344 (78.49%), Postives = 295/344 (85.76%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           M+  SV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLP--PVIFYFHGGGFAFGSAAA 120
           +VSSSDLTID SRDLFLRIFTP   A       A D  LP  P+IFYFHGGGFAFGSA A
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTA-------ALDESLPLLPIIFYFHGGGFAFGSADA 120

Query: 121 TSMDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADL 180
           TS DMAAR F+++L AV+ISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DL
Sbjct: 121 TSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDL 180

Query: 181 SRCFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPL 240
           SRCFILGESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PL
Sbjct: 181 SRCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPL 240

Query: 241 TLKLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRY 300
           TL + DWFW+AFLPAGEDRDHAAANVNGPNGRD+SGL NFPAT+I AGGLDLL+DRQ+ Y
Sbjct: 241 TLYMTDWFWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSY 300

Query: 301 YEELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342
           YE LKRMGK+VKLVVF+N  HGFF F DLPEYSLMI+EM DFIA
Sbjct: 301 YERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 336

BLAST of Cp4.1LG06g09420 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 671 bits (1731), Expect = 3.14e-243
Identity = 333/349 (95.42%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQPFSVSSEISTSQLPWKHRLTLRFAALLFNAS+RSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTIDASRDLFLRIFTPT        VAATDSLLPPVIFYFHGGGFAFGSAAATS
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPT--------VAATDSLLPPVIFYFHGGGFAFGSAAATS 120

Query: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAV+ISVNYRL+PQFRFPCQYDDGFDAVKFIDEMDD+DPLLG+ADLSR
Sbjct: 121 MDMAARRFSKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLD LIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349
           ELKRMGK+VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI MFHRNIL
Sbjct: 301 ELKRMGKDVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIGMFHRNIL 341

BLAST of Cp4.1LG06g09420 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 667 bits (1722), Expect = 7.40e-242
Identity = 334/349 (95.70%), Postives = 337/349 (96.56%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           MQP SVSSEISTSQLPWKHRLTLRFAALLFNAS+RSDLTVNRRLLNFLDPKIPPNPNSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120
           AVSSSDLTID SRDLFLRIFTPT        VAATDS+LPPVIFYFHGGGFAFGSA ATS
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT--------VAATDSVLPPVIFYFHGGGFAFGSADATS 120

Query: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180
           MDMAARRFSKELGAV+ISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR
Sbjct: 121 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300
           KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300

Query: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIAMFHRNIL 349
           ELKRMGKEVKLVVFTNGVHGFFSFED PEYSLMIKEMRDFIAMFHRNIL
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIAMFHRNIL 341

BLAST of Cp4.1LG06g09420 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 544 bits (1401), Expect = 5.52e-193
Identity = 270/344 (78.49%), Postives = 295/344 (85.76%), Query Frame = 0

Query: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60
           M+  SV  E+ST QLPWKH++TLRFA LLFN S+RSD TVNRRLL FLDPKIPPNPNS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLP--PVIFYFHGGGFAFGSAAA 120
           +VSSSDLTID SRDLFLRIFTP   A       A D  LP  P+IFYFHGGGFAFGSA A
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTA-------ALDESLPLLPIIFYFHGGGFAFGSADA 120

Query: 121 TSMDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADL 180
           TS DMAAR F+++L AV+ISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DL
Sbjct: 121 TSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDL 180

Query: 181 SRCFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPL 240
           SRCFILGESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PL
Sbjct: 181 SRCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPL 240

Query: 241 TLKLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRY 300
           TL + DWFW+AFLPAGEDRDHAAANVNGPNGRD+SGL NFPAT+I AGGLDLL+DRQ+ Y
Sbjct: 241 TLYMTDWFWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSY 300

Query: 301 YEELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342
           YE LKRMGK+VKLVVF+N  HGFF F DLPEYSLMI+EM DFIA
Sbjct: 301 YERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 336

BLAST of Cp4.1LG06g09420 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 540 bits (1390), Expect = 2.52e-191
Identity = 268/339 (79.06%), Postives = 293/339 (86.43%), Query Frame = 0

Query: 6   VSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSS 65
           V  +IST QLPWKHR+TLRFA LL+N S+RSDLTVNRRLLNF+DPKIP N +S H+VSSS
Sbjct: 6   VCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTHSVSSS 65

Query: 66  DLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLP--PVIFYFHGGGFAFGSAAATSMDM 125
           DL +D SRDLFLRIFTPT  A       A D  LP  P+IFYFHGGGF FGSA ATS DM
Sbjct: 66  DLIVDTSRDLFLRIFTPTPTA-------ALDESLPLLPIIFYFHGGGFVFGSADATSTDM 125

Query: 126 AARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFI 185
           AARRF+K+L AV+ISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDDD  LL + DLS CFI
Sbjct: 126 AARRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDS-LLERVDLSCCFI 185

Query: 186 LGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLA 245
           LGESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + 
Sbjct: 186 LGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMT 245

Query: 246 DWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELK 305
           DWFW+AFLPAGEDRDHAAANVNGPNGRD+SGL NFPAT+I AGGLDLLIDRQ+ YYE LK
Sbjct: 246 DWFWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLK 305

Query: 306 RMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342
           RMGK+VKLVVF+N VHG+F F DLPEYSLMIKEMRDFIA
Sbjct: 306 RMGKDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

BLAST of Cp4.1LG06g09420 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 535 bits (1379), Expect = 1.19e-189
Identity = 266/339 (78.47%), Postives = 291/339 (85.84%), Query Frame = 0

Query: 6   VSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSS 65
           V  +IST QLPWKHR+TLRFA LL+N S+RSDLTVNRRLLNF+DPKIP N +S H+VSSS
Sbjct: 6   VCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTHSVSSS 65

Query: 66  DLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLP--PVIFYFHGGGFAFGSAAATSMDM 125
           DL +D SRDLFLRIFTPT  A       A D  LP  P+IFYFHGGGF FGSA ATS DM
Sbjct: 66  DLIVDTSRDLFLRIFTPTPTA-------ALDESLPLLPIIFYFHGGGFVFGSADATSTDM 125

Query: 126 AARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFI 185
           AARRF+K+L AV+ISVNYRLAP+FRFPCQ DDGFD +KFIDEMDDD  LL + DLSRCFI
Sbjct: 126 AARRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDS-LLERVDLSRCFI 185

Query: 186 LGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLA 245
           LGESAGGNLGHHVAVRASEY FKRVK+IGFIASQPFFGG+ERTESE RLC Q PLTL + 
Sbjct: 186 LGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMT 245

Query: 246 DWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELK 305
           DWFW+AFLPAGEDRDHA ANVNGPNGRD+SGL NFPAT+I AGGLDLLIDRQ+ YYE LK
Sbjct: 246 DWFWRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLK 305

Query: 306 RMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342
           RMGK+VKLVVF+N VHG+F F DLPEYSLMIKEMRDFIA
Sbjct: 306 RMGKDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIA 336

BLAST of Cp4.1LG06g09420 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 289.7 bits (740), Expect = 3.2e-78
Identity = 153/330 (46.36%), Postives = 208/330 (63.03%), Query Frame = 0

Query: 15  LPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRD 74
           LP K R+ L   + + + + R D T+NRR L   D + PPNP  ++ VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGA 134
           L+ R++TP           + D +  PV+ +FHGGGFAF S  A   D   RRF+++L A
Sbjct: 73  LWFRLYTPH---------VSGDKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPA 132

Query: 135 VIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGH 194
            +ISVNYRLAP+ R+P QYDDGFDA+K+I+E +    L   ADLSRCF  G+SAGGN+ H
Sbjct: 133 YVISVNYRLAPEHRYPAQYDDGFDALKYIEE-NHGSILPANADLSRCFFAGDSAGGNIAH 192

Query: 195 HVAV---RASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFL 254
           +VA+   R    +F  VK+IG I+ QPFFGGEERTE+E +L   P ++    DW WKA  
Sbjct: 193 NVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM- 252

Query: 255 PAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKL 314
             G +RDH A NV GPN  D+SGL ++P T+++  G D L D QR YYE LK  GK+  L
Sbjct: 253 --GLNRDHEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATL 312

Query: 315 VVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 342
           + + N  H F+ F +LPE   +I  ++DF+
Sbjct: 313 IEYPNMFHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Cp4.1LG06g09420 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 130/318 (40.88%), Postives = 186/318 (58.49%), Query Frame = 0

Query: 28  LLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTDAAI 87
           L +N   R D T NR L  FLD K+P N N ++ V S D+ ID   +L  R++ P DA  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  NAAAV---AATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVNYRLA 147
           + +        D  + PVI +FHGG FA  SA +   D   RR     GAV++SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 207
           P+ R+PC YDDG+  +K++   +    L  K D   R F+ G+S+GGN+ H+VAVRA E 
Sbjct: 149 PENRYPCAYDDGWAVLKWV---NSSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE- 208

Query: 208 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 267
              R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GEDR+H A +
Sbjct: 209 --SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACS 268

Query: 268 VNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 327
             GP  + + GL +FP ++++  GLDL+ D Q +Y E LK+ G+EVKL+       GF+ 
Sbjct: 269 PFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYL 328

Query: 328 FEDLPEYSLMIKEMRDFI 342
             +   +  ++ E+  F+
Sbjct: 329 LPNNNHFHTVMDEIAAFV 339

BLAST of Cp4.1LG06g09420 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 4.2e-54
Identity = 120/318 (37.74%), Postives = 173/318 (54.40%), Query Frame = 0

Query: 30  FNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTDAAINA 89
           +N   R D T NR L  +LD K+  N N +  V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  A-----AVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVNYRLA 149
                      D  + PVI +FHGG FA  SA +   D   RR       V++SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLS-RCFILGESAGGNLGHHVAVRASEY 209
           P+  +PC YDDG+ A+ +++       L  K D     F+ G+S+GGN+ H+VA+RA E 
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR---SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE- 210

Query: 210 AFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN 269
               + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GEDR+H A N
Sbjct: 211 --SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACN 270

Query: 270 VNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFS 329
              P G+ + G+ +FP ++++  GLDL+ D Q  Y E LK+ G+EVKL+       GF+ 
Sbjct: 271 PFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYL 330

Query: 330 FEDLPEYSLMIKEMRDFI 342
             +   +  ++ E+  F+
Sbjct: 331 LPNNNHFHNVMDEISAFV 341

BLAST of Cp4.1LG06g09420 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.1e-53
Identity = 115/321 (35.83%), Postives = 179/321 (55.76%), Query Frame = 0

Query: 28  LLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTD--- 87
           L +    R D + NR L  FLD K+P N   +  V S D  +D++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ----AAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGAVIISVN 147
                 +      +T  ++ PV+ +FHGG F   SA +   D   RR     G V++SV+
Sbjct: 89  QTRHGTLELTKPLSTTEIV-PVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVD 148

Query: 148 YRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGHHVAVRA 207
           YR +P+ R+PC YDDG++A+ ++          GK      ++ G+S+GGN+ H+VAVRA
Sbjct: 149 YRRSPEHRYPCAYDDGWNALNWVKSRVWLQS--GKDSNVYVYLAGDSSGGNIAHNVAVRA 208

Query: 208 SEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHA 267
           +    + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GEDRDH 
Sbjct: 209 TN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHP 268

Query: 268 AANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHG 327
           A N  GP G+ + G+ NFP ++++  GLDL+ D Q  Y + LK+ G EV L+       G
Sbjct: 269 ACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIG 328

Query: 328 FFSFEDLPEYSLMIKEMRDFI 342
           F+   +   +  +++E+  F+
Sbjct: 329 FYFLPNNDHFHCLMEELNKFV 341

BLAST of Cp4.1LG06g09420 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 7.8e-40
Identity = 99/287 (34.49%), Postives = 147/287 (51.22%), Query Frame = 0

Query: 50  PKIPPNPNSIHAVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGG 109
           PK+PP         S D+ ++ + + F+RIF P +           +S L P++ YFHGG
Sbjct: 31  PKLPPTE------QSKDIPLNQTNNTFIRIFKPRN--------IPPESKL-PILVYFHGG 90

Query: 110 GFAFGSAAATSMDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEM--- 169
           GF   SAA+     +  + +  L  +I+SV YRLAP+ R P  Y+D  +A+ ++ +    
Sbjct: 91  GFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPAAYEDAVEAILWLRDQARG 150

Query: 170 -----DDDDPLLGKADLSRCFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFG 229
                D D  L    D S+C+++G S+GGN+ ++VA+R  +     VK+ G I +Q FFG
Sbjct: 151 PINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDTDLSPVKIQGLIMNQAFFG 210

Query: 230 GEERTESEIRLCNQPPLTLKLADWFWKAFLPAGEDRDHAAAN---VNGPNGRDVSGLRNF 289
           G E ++SE RL +     L      W   LP G DRDH  +N    +GP  +D  G   F
Sbjct: 211 GVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSNPIKSSGPQEKDKMG--RF 270

Query: 290 PATIILAGGLDLLIDRQRRYYEELKRMGKEVKLVVFTNGVHGFFSFE 326
           P+T+I   G D L+DRQR   E LK  G  V+     +G H    F+
Sbjct: 271 PSTLINGYGGDPLVDRQRHVAEMLKGRGVHVETRFDKDGFHACELFD 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT104.5e-7746.36Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G61.6e-6140.88Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5459.5e-5942.33Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9MAA76.0e-5337.74Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC13.0e-5235.83Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023536262.11.13e-254100.00probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
KAG6592331.11.94e-24496.28putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022973499.16.49e-24395.42probable carboxylesterase 18 [Cucurbita maxima][more]
XP_022925491.11.53e-24195.70probable carboxylesterase 18 [Cucurbita moschata][more]
XP_004141618.11.14e-19278.49probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
A0A6J1IER73.14e-24395.42probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
A0A6J1ECC87.40e-24295.70probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A0A0KXS85.52e-19378.49Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1[more]
A0A1S3CBA42.52e-19179.06probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1[more]
A0A5D3BL911.19e-18978.47Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
AT5G23530.13.2e-7846.36carboxyesterase 18 [more]
AT5G27320.11.1e-6240.88alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.14.2e-5437.74alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.12.1e-5335.83alpha/beta-Hydrolases superfamily protein [more]
AT2G45600.17.8e-4034.49alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 4..342
e-value: 1.7E-85
score: 289.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 45..341
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 103..322
e-value: 1.8E-50
score: 171.8
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 10..344
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 10..344
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 103..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g09420.1Cp4.1LG06g09420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity