Cp4.1LG06g08750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG06g08750 vs. ExPASy Swiss-Prot
Match: D2WL32 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SBE3 PE=1 SV=1)

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 645/905 (71.27%), Postives = 737/905 (81.44%), Query Frame = 0

Query: 9   MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KL+I C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDRHANLQDLS 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER+K+LKDLKDEI+ RH + +D +
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           SGF  LGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LEDKL+ GE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGSEPKV 428
           R+PAWATYVQP+ EGKQ +AIHWEP PE AYKWK + P VP++L+IYECHVGISGSEPKV
Sbjct: 361 RVPAWATYVQPEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISGSEPKV 420

Query: 429 SSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLV 488
           S+F+ F +KVLPHVK AGYNAIQL GV EHKDYFT+GYRVTNFFA SSRYGTP+DFKRLV
Sbjct: 421 STFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDDFKRLV 480

Query: 489 DEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQD 548
           DEAHGLGLLVFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFKYGD D
Sbjct: 481 DEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLD 540

Query: 549 VLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLM 608
           VLHFL+SNLNWW+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DAL+YL+
Sbjct: 541 VLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDALMYLI 600

Query: 609 LANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDHE 668
           LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L NVPD+E
Sbjct: 601 LANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNE 660

Query: 669 WSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETLLRGC 728
           WSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE L RG 
Sbjct: 661 WSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRGI 720

Query: 729 SLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEEIHRD 788
           SLHKMIR ITFT GG+AYLNFMGNEFGHP+RVEFP  SNN SFSLANR+WD LE  +H  
Sbjct: 721 SLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHH 780

Query: 789 LFVFDK---------------------------VISYIRGPFLFICNFHPTDSYESYSVG 848
           LF FDK                           VIS+ RGPFLFI NFHP++SYE Y VG
Sbjct: 781 LFSFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVG 840

Query: 849 VEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLS 883
           VEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLEV LP RTAQVYKL+
Sbjct: 841 VEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLEVFLPSRTAQVYKLT 899

BLAST of Cp4.1LG06g08750 vs. ExPASy Swiss-Prot
Match: Q8NKE1 (1,4-alpha-glucan-branching enzyme OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=GLC3 PE=2 SV=2)

HSP 1 Score: 484.6 bits (1246), Expect = 2.4e-135
Identity = 258/643 (40.12%), Postives = 378/643 (58.79%), Query Frame = 0

Query: 268 LEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNGKEYDIFNVVIDPVTREKFKVKKPPIPY 327
           L  +P+ +  Y+ W       P++V+  +I    ++D       P+ ++ F V +  IP 
Sbjct: 63  LNVLPNGDIIYREWA------PNAVAASLIGEFNDWDRSK---HPMKKDSFGVWEVHIP- 122

Query: 328 WLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAEGKQGF-AI 387
                       K   PTIPH +K ++   TP+G  ++R+PAW   V  D      + AI
Sbjct: 123 -----------AKNGIPTIPHNTKIKISMTTPEGECIDRLPAWIKRVTQDLNVSLAYDAI 182

Query: 388 HWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGSEPKVSSFDYFREKVLPHVKEAGYNA 447
            W PP +  Y+WKN SP  P +L+IYE HVGIS +E +V +++ F + VL  +K+ GYNA
Sbjct: 183 FWNPPQK--YQWKNNSPKKPTSLRIYEAHVGISTNEGRVGTYNEFTDNVLKRIKDLGYNA 242

Query: 448 IQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLLVFLDIVHSYAAA 507
           IQL  ++EH  Y + GY+VT+FF VSSRYGTPE+  RL+D AHG+GL V LD+VHS+A  
Sbjct: 243 IQLMAIMEHAYYASFGYQVTSFFGVSSRYGTPEELMRLIDTAHGMGLYVLLDVVHSHACK 302

Query: 508 DEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVLHFLLSNLNWWVVEYRIDGF 567
           + + GL+ FDGS+ CYFH G +G H  W +R+F YG  +VL FLLSNL +++ EYR DGF
Sbjct: 303 NVLDGLNMFDGSDHCYFHEGGKGRHDLWDSRLFNYGHWEVLRFLLSNLRFFMEEYRFDGF 362

Query: 568 RFHSLSSMIYTHNGFAS-FTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDA 627
           RF  ++SM+Y H+G  + F+G   E+    VD+  ++YLMLAN++LH L+P IIT++ED 
Sbjct: 363 RFDGVTSMMYHHHGIGTGFSGGYHEYFGDTVDEGGVVYLMLANDMLHKLYPRIITVSEDV 422

Query: 628 TYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDHEWSMNKIVSSLIDNRHYVNKM 687
           +  PGLC P  +GG+GFDY + ++  +MW   L+   D +W M  I  +L + RH + K 
Sbjct: 423 SGMPGLCLPVEEGGIGFDYRLAMAIPDMWIKLLKEQRDDDWDMGNICWTLTNRRH-MEKT 482

Query: 688 LLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETLL-----RGCSLHKMIRFITFTMGG 747
           + YAE+H Q++ G ++ A  L  D + +   S  T L     RG +LHKMIR +T  +GG
Sbjct: 483 IAYAESHDQALVGDKTLAFWLM-DKEMYTHMSDMTPLTPIIDRGLALHKMIRLLTHGLGG 542

Query: 748 QAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEEIHRDLFV--FD-------- 807
           + YLNF GNEFGHP+ ++FP   NN SF  A RQW+ +++++ R  ++  FD        
Sbjct: 543 EGYLNFEGNEFGHPEWLDFPRAGNNNSFHYARRQWNVVDDDLLRYKYLNEFDKAMQHLEE 602

Query: 808 -------------------KVISYIRGPFLFICNFHPTDSYESYSVGVEEAGEYRIILNT 867
                              K++++ RG  L+I NFHPT S+  Y +G E AG+Y I LNT
Sbjct: 603 QYGWLSSPQAYISLKHNENKLVAFERGNLLWIFNFHPTQSFADYKIGTEWAGKYSIALNT 662

Query: 868 DETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 874
           D   +GG   I  +     +     DG +N ++V +PCR A V
Sbjct: 663 DRKIFGGHDRI-DESISYHSQPHEWDGRKNYIQVYIPCRVALV 679

BLAST of Cp4.1LG06g08750 vs. ExPASy Swiss-Prot
Match: P30924 (1,4-alpha-glucan-branching enzyme OS=Solanum tuberosum OX=4113 GN=SBE1 PE=2 SV=2)

HSP 1 Score: 473.8 bits (1218), Expect = 4.3e-132
Identity = 234/541 (43.25%), Postives = 327/541 (60.44%), Query Frame = 0

Query: 344 PTIPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAE--GKQGFAIHWEPPPEYAYKWKNT 403
           P IPH S+ +  F   +G  ++RIPAW  Y   DA         ++W+PPP   Y +K  
Sbjct: 199 PVIPHNSRVKFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWDPPPSERYHFKYP 258

Query: 404 SPNVPKALKIYECHVGISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTL 463
            P  P+A +IYE HVG+S SEP+V+S+  F + VLP +K   YN +QL  ++EH  Y + 
Sbjct: 259 RPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQLMAIMEHSYYGSF 318

Query: 464 GYRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFD---GS 523
           GY VTNFFAVSSRYG PED K L+D+AH LGL V +D+VHS+A+ +   GL+ FD   GS
Sbjct: 319 GYHVTNFFAVSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTDGLNGFDIGQGS 378

Query: 524 NDCYFHTGKRGHHKYWGTRMFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTH 583
            + YFH G+RG+HK W +R+F Y + +VL FLLSNL WW+ EY  DGFRF  ++SM+Y H
Sbjct: 379 QESYFHAGERGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGFRFDGITSMLYVH 438

Query: 584 NGF-ASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQ 643
           +G    FTG+  E+ ++  D DA++YLMLAN ++H + P+   IAED +  PGL  P S+
Sbjct: 439 HGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDVSGMPGLGRPVSE 498

Query: 644 GGLGFDYYVNLSASEMWSSFLQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSIS 703
           GG+GFDY + ++  + W  +L+N  D +WSM ++ SSL  NR Y  K + YAE+H QSI 
Sbjct: 499 GGIGFDYRLAMAIPDKWIDYLKNKNDEDWSMKEVTSSL-TNRRYTEKCIAYAESHDQSIV 558

Query: 704 GGRSYAEILFGDTKEHG----AGSKETLLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGH 763
           G ++ A +L       G      +   + RG +LHKMI F T  +GG+ YLNFMGNEFGH
Sbjct: 559 GDKTIAFLLMDKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEGYLNFMGNEFGH 618

Query: 764 PKRVEFPMPSNNLSFSLANRQWDFLEEEIHRDLFV--FD--------------------- 823
           P+ ++FP   NN S+    RQW+  + E  R  F+  FD                     
Sbjct: 619 PEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLDEKFSFLASGKQIVS 678

Query: 824 ------KVISYIRGPFLFICNFHPTDSYESYSVGVEEAGEYRIILNTDETKYGGQGIIKH 845
                 KV+ + RG  +F+ NFHP ++YE Y VG +  G+YR+ L++D  ++GG G   H
Sbjct: 679 SMDDDNKVVVFERGDLVFVFNFHPKNTYEGYKVGCDLPGKYRVALDSDAWEFGGHGRTGH 738

BLAST of Cp4.1LG06g08750 vs. ExPASy Swiss-Prot
Match: Q6T308 (1,4-alpha-glucan-branching enzyme OS=Felis catus OX=9685 GN=GBE1 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 3.6e-131
Identity = 234/534 (43.82%), Postives = 331/534 (61.99%), Query Frame = 0

Query: 346 IPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNV 405
           +PHGSK +V   +  G  L RI  WA YV  + E       HW+  PE+ YK+K++ P  
Sbjct: 133 VPHGSKLKVVIRSKSGEILYRISPWAKYVTREGENVNYDWTHWD--PEHPYKFKHSRPKK 192

Query: 406 PKALKIYECHVGISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRV 465
           P+ ++IYE HVGIS  E K++S+ +F   VLP +K+ GYN IQ+  ++EH  Y + GY++
Sbjct: 193 PRGVRIYESHVGISSYEGKIASYKHFTYNVLPRIKDLGYNCIQMMAIMEHAYYASFGYQI 252

Query: 466 TNFFAVSSRYGTPEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHT 525
           T+FFA SSRYGTPE+ K LVD AH +G+ V LD+VHS+A+ +   GL+ FDG++ CYFH+
Sbjct: 253 TSFFAASSRYGTPEELKELVDTAHSMGITVLLDVVHSHASKNSEDGLNMFDGTDSCYFHS 312

Query: 526 GKRGHHKYWGTRMFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFA-SF 585
           G RG+H  W +R+F Y   +VL FLLSN+ WW+ EY  DGFRF  ++SM+Y H+G   +F
Sbjct: 313 GPRGNHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYGFDGFRFDGVTSMLYHHHGMGQAF 372

Query: 586 TGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDY 645
           +GD  E+    VD+DAL+YLMLAN ++H L+PN ITIAED +  P LC P SQGG+GFDY
Sbjct: 373 SGDYHEYFGLQVDEDALIYLMLANHLVHTLYPNSITIAEDVSGMPALCSPISQGGVGFDY 432

Query: 646 YVNLSASEMWSSFLQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAE 705
            + ++  + W   L+   D +W+M  IV +L  NR Y+ K + YAE+H Q++ G ++ A 
Sbjct: 433 RLAMAIPDKWIQLLKEFKDEDWNMGNIVYTL-TNRRYLEKCIAYAESHDQALVGDKTLAF 492

Query: 706 ILFGDTKEHGAGSKET-----LLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEF 765
            L  D + +   S  T     + RG  LHKMIR IT  +GG+ YLNFMGNEFGHP+ ++F
Sbjct: 493 WLM-DAEMYTNMSVLTPFTPVIDRGIQLHKMIRLITHALGGEGYLNFMGNEFGHPEWLDF 552

Query: 766 PMPSNNLSFSLANRQWDFLEEEIHRDLFV--FD--------------------------- 825
           P   NN S+  A RQ+   ++++ R  F+  FD                           
Sbjct: 553 PRKGNNESYHYARRQFHLTDDDLLRYKFLNNFDRDMNKLEERCGWLSAPQAFVSEKHEGN 612

Query: 826 KVISYIRGPFLFICNFHPTDSYESYSVGVEEAGEYRIILNTDETKYGGQGIIKH 844
           K+I++ R   +FI NFHP+ SY  Y VG    G++RI+L+TD  +YGG   + H
Sbjct: 613 KIIAFERAGLVFIFNFHPSKSYTDYRVGTTLPGKFRIVLDTDAAEYGGHQRLDH 662

HSP 2 Score: 59.3 bits (142), Expect = 2.5e-07
Identity = 31/96 (32.29%), Postives = 45/96 (46.88%), Query Frame = 0

Query: 97  KQFAHFLRERYKSLKDLKDEIYDRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYC 156
           K FA   + RYK   +  + I +    +   S G+   G+HR  +  +   EWAPGA   
Sbjct: 36  KPFALDFQRRYKKFNETLNNIGENEGGIDKFSRGYESFGVHRCADGGLYCKEWAPGAEGV 95

Query: 157 ALIGDFNDWSPRENVAREGHLGHDDYGYWFIILEDK 193
            L GDFNDW+P     ++      DYG W + +  K
Sbjct: 96  FLTGDFNDWNPFSYPYKK-----LDYGKWELYIPPK 126

BLAST of Cp4.1LG06g08750 vs. ExPASy Swiss-Prot
Match: Q9D6Y9 (1,4-alpha-glucan-branching enzyme OS=Mus musculus OX=10090 GN=Gbe1 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.8e-130
Identity = 243/568 (42.78%), Postives = 340/568 (59.86%), Query Frame = 0

Query: 343 NPTIPHGSKYRVYFNTPDGP-LERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTS 402
           +P IPHGSK +V   +  G  L RI  WA YV  +        IHW   PE  YK+K++ 
Sbjct: 133 SPLIPHGSKLKVVITSKSGEILYRISPWAKYVVRENNNVNYDWIHW--APEDPYKFKHSR 192

Query: 403 PNVPKALKIYECHVGISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLG 462
           P  P++L+IYE HVGIS  E K++S+ +F   VLP +K+ GYN IQL  ++EH  Y + G
Sbjct: 193 PKKPRSLRIYESHVGISSHEGKIASYKHFTSNVLPRIKDLGYNCIQLMAIMEHAYYASFG 252

Query: 463 YRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCY 522
           Y++T+FFA SSRYGTPE+ K LVD AH +G++V LD+VHS+A+ +   GL+ FDG++ CY
Sbjct: 253 YQITSFFAASSRYGTPEELKELVDTAHSMGIVVLLDVVHSHASKNSEDGLNMFDGTDSCY 312

Query: 523 FHTGKRGHHKYWGTRMFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFA 582
           FH+G RG H  W +R+F Y   +VL FLLSN+ WW+ EY  DGFRF  ++SM+Y H+G  
Sbjct: 313 FHSGPRGTHDLWDSRLFIYSSWEVLRFLLSNIRWWLEEYCFDGFRFDGVTSMLYHHHGMG 372

Query: 583 -SFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLG 642
             F+GD  E+    VD+DAL+YLMLAN + H L+P+ ITIAED +  P LC PTSQGG G
Sbjct: 373 QGFSGDYNEYFGLQVDEDALIYLMLANHLAHTLYPDSITIAEDVSGMPALCSPTSQGGGG 432

Query: 643 FDYYVNLSASEMWSSFLQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRS 702
           FDY + ++  + W   L+   D +W+M  IV +L  NR Y+ K + YAE+H Q++ G ++
Sbjct: 433 FDYRLAMAIPDKWIQLLKEFKDEDWNMGNIVYTL-TNRRYLEKCVAYAESHDQALVGDKT 492

Query: 703 YAEILFGDTKEHG-----AGSKETLLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKR 762
            A  L  D + +      A     + RG  LHKMIR IT  +GG+ YLNFMGNEFGHP+ 
Sbjct: 493 LAFWLM-DAEMYTNMSVLAPFTPVIDRGIQLHKMIRLITHGLGGEGYLNFMGNEFGHPEW 552

Query: 763 VEFPMPSNNLSFSLANRQWDFLEEEIHRDLFV--FD------------------------ 822
           ++FP   NN S+  A RQ++  ++++ R  F+  FD                        
Sbjct: 553 LDFPRKGNNESYHYARRQFNLTDDDLLRYKFLNNFDRDMNRLEERCGWLSAPQAYVSEKH 612

Query: 823 ---KVISYIRGPFLFICNFHPTDSYESYSVGVEEAGEYRIILNTDETKYGGQGIIKHD-Q 874
              K I++ R   LFI NFHP+ SY  Y VG    G+++I+L++D  +YGG   + H+  
Sbjct: 613 EANKTITFERAGLLFIFNFHPSKSYTDYRVGTATPGKFKIVLDSDAAEYGGHQRLDHNTN 672

BLAST of Cp4.1LG06g08750 vs. NCBI nr
Match: XP_023535701.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo subsp. pepo] >XP_023535702.1 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo subsp. pepo] >XP_023535703.1 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1826 bits (4729), Expect = 0.0
Identity = 882/909 (97.03%), Postives = 882/909 (97.03%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60
           MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180
           HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720
           PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL
Sbjct: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720

Query: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780
           LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE
Sbjct: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780

Query: 781 IHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYES 840
           IHRDLFVFDK                           VISYIRGPFLFICNFHPTDSYES
Sbjct: 781 IHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 882
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Cp4.1LG06g08750 vs. NCBI nr
Match: XP_022937279.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita moschata])

HSP 1 Score: 1805 bits (4676), Expect = 0.0
Identity = 870/909 (95.71%), Postives = 879/909 (96.70%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60
           MNQHSFPAMNALSSSPS+KFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180
           HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA+IGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILED+LK GEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EP+VSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPEVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720
           PD EWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHG+GSKETL
Sbjct: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720

Query: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780
           LRGCSLHKMIRFITFTMGG+AYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWD LE+E
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEKE 780

Query: 781 IHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYES 840
           +HRDLFVFDK                           VISYIRGPFLFICNFHPTDSYES
Sbjct: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 882
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Cp4.1LG06g08750 vs. NCBI nr
Match: KAG6592053.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1799 bits (4660), Expect = 0.0
Identity = 866/909 (95.27%), Postives = 874/909 (96.15%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60
           MNQHSFPAMNALSSSPS+KFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRI+CSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180
           HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA+IGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILED+LK GEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAY WKNT PNVPKALKIYECHVGISG 
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYNWKNTRPNVPKALKIYECHVGISGP 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGL VFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLFVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720
           PD EWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHG+GSKETL
Sbjct: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720

Query: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780
           LRGCSLHKMIRFITFTMGG+AYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWD LE+E
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEKE 780

Query: 781 IHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYES 840
           +HRDLFVFDK                           VISYIRGPFLFICNFHPTDSYE 
Sbjct: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYER 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 882
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Cp4.1LG06g08750 vs. NCBI nr
Match: XP_022976653.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita maxima] >XP_022976654.1 1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucurbita maxima])

HSP 1 Score: 1798 bits (4657), Expect = 0.0
Identity = 867/909 (95.38%), Postives = 874/909 (96.15%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60
           MNQHSFP MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRI+CSASEHSQ
Sbjct: 1   MNQHSFPPMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQ AHFLRERYKSLKDLKDEIYDR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQLAHFLRERYKSLKDLKDEIYDR 120

Query: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180
           HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA+IGDFN WSPRE+VAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNGWSPREHVAREGHLGHD 180

Query: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILEDKLK GEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDKLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFK KKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKAKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGLLV LDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVLLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720
           PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL
Sbjct: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720

Query: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780
           LRGCSLHKMIRFITFTMGG+AYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWD LEE+
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEEK 780

Query: 781 IHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYES 840
           +HRDLFVFDK                           VISYIRGPFLFICNFHPTDSYES
Sbjct: 781 MHRDLFVFDKDLMGLGENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 882
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHD YLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDHYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Cp4.1LG06g08750 vs. NCBI nr
Match: XP_004139870.1 (1,4-alpha-glucan-branching enzyme 3, chloroplastic/amyloplastic [Cucumis sativus] >KAE8646063.1 hypothetical protein Csa_016038 [Cucumis sativus])

HSP 1 Score: 1658 bits (4294), Expect = 0.0
Identity = 805/907 (88.75%), Postives = 836/907 (92.17%), Query Frame = 0

Query: 9   MNALSSSPS----IKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQHAEP 68
           MNALSSSPS    IKF PYKT H LLLP  RSTLQSI+LS K+KL++ CSASEH QHAEP
Sbjct: 1   MNALSSSPSPSPSIKFSPYKT-HSLLLPQPRSTLQSISLSGKVKLKVSCSASEHPQHAEP 60

Query: 69  AKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDRHANL 128
            K Q SKK K+VSDGEKGVDPVGFLTKLGISHKQFA +LRERYKSLKDLKDEI++RHANL
Sbjct: 61  RKRQPSKKAKNVSDGEKGVDPVGFLTKLGISHKQFAQYLRERYKSLKDLKDEIFNRHANL 120

Query: 129 QDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHDDYGY 188
            DLSSGFMFLGMHRH+EHRVDFMEWAPGARYCA++GDFN WSPREN AREGH GHDDYGY
Sbjct: 121 TDLSSGFMFLGMHRHMEHRVDFMEWAPGARYCAVVGDFNGWSPRENAAREGHFGHDDYGY 180

Query: 189 WFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKN 248
           WFIILEDKLK GEKP+ELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKN
Sbjct: 181 WFIILEDKLKEGEKPDELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKN 240

Query: 249 RFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVIDNGKE 308
           RFEVPAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SYDVIDNGKE
Sbjct: 241 RFEVPAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSNVPSYDVIDNGKE 300

Query: 309 YDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGP 368
           YDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKY+PTIPHGSKYRVYFNTP+GP
Sbjct: 301 YDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYSPTIPHGSKYRVYFNTPNGP 360

Query: 369 LERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGSEP 428
           LERIPAWATYVQPDA GKQGFAIHWEPPPE+AYKWKNT PNVPKALKIYECHVGISGSEP
Sbjct: 361 LERIPAWATYVQPDAHGKQGFAIHWEPPPEHAYKWKNTKPNVPKALKIYECHVGISGSEP 420

Query: 429 KVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKR 488
           +VSSF+YF EKVLPHVKEAGYNAIQLFGVVEHKDYFT+GYRVTNFFAVSSRYGTPEDFKR
Sbjct: 421 RVSSFNYFIEKVLPHVKEAGYNAIQLFGVVEHKDYFTVGYRVTNFFAVSSRYGTPEDFKR 480

Query: 489 LVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGD 548
           LVDEAHGLGLLVFLDIVHSYA+ADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGD
Sbjct: 481 LVDEAHGLGLLVFLDIVHSYASADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGD 540

Query: 549 QDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLY 608
           QDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLY
Sbjct: 541 QDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLY 600

Query: 609 LMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPD 668
           L+LANEILHGLHP+IITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMWSSFLQNVPD
Sbjct: 601 LILANEILHGLHPDIITIAEDATYYPGLCEPISQGGLGFDYYVNLSASEMWSSFLQNVPD 660

Query: 669 HEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETLLR 728
            EW+MNKIVSSLI NRH  NKMLL+AENH QSISGGRSYAEILFGD KEHGAGSKETLLR
Sbjct: 661 QEWNMNKIVSSLIGNRHSTNKMLLFAENHGQSISGGRSYAEILFGDIKEHGAGSKETLLR 720

Query: 729 GCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEEIH 788
           GCSLHKMIR ITFT+GG+AYLNFMGNEFGHPKRVEFPMPSNN SFSLANRQWD LE+E+H
Sbjct: 721 GCSLHKMIRLITFTIGGRAYLNFMGNEFGHPKRVEFPMPSNNFSFSLANRQWDLLEKEMH 780

Query: 789 RDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYESYS 848
            DLF+FDK                           VISYIRGPFLFI NFHPTDS+E YS
Sbjct: 781 HDLFLFDKELMGLDENEKILTRSLPNVHHVNETTKVISYIRGPFLFIYNFHPTDSFERYS 840

Query: 849 VGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYK 882
           VGVEEAGEYRIILNTDE +YGGQG IKHDQYLQRTISRRIDGLRNCLEV LPCRTAQVYK
Sbjct: 841 VGVEEAGEYRIILNTDEIEYGGQGNIKHDQYLQRTISRRIDGLRNCLEVSLPCRTAQVYK 900

BLAST of Cp4.1LG06g08750 vs. ExPASy TrEMBL
Match: A0A6J1FAQ6 (1,4-alpha-glucan branching enzyme OS=Cucurbita moschata OX=3662 GN=LOC111443613 PE=3 SV=1)

HSP 1 Score: 1805 bits (4676), Expect = 0.0
Identity = 870/909 (95.71%), Postives = 879/909 (96.70%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60
           MNQHSFPAMNALSSSPS+KFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ
Sbjct: 1   MNQHSFPAMNALSSSPSLKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120

Query: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180
           HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA+IGDFNDWSPRENVAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNDWSPRENVAREGHLGHD 180

Query: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILED+LK GEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDRLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EP+VSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPEVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720
           PD EWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHG+GSKETL
Sbjct: 661 PDQEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGSGSKETL 720

Query: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780
           LRGCSLHKMIRFITFTMGG+AYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWD LE+E
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEKE 780

Query: 781 IHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYES 840
           +HRDLFVFDK                           VISYIRGPFLFICNFHPTDSYES
Sbjct: 781 MHRDLFVFDKDLMGLDENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 882
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Cp4.1LG06g08750 vs. ExPASy TrEMBL
Match: A0A6J1IP54 (1,4-alpha-glucan branching enzyme OS=Cucurbita maxima OX=3661 GN=LOC111476992 PE=3 SV=1)

HSP 1 Score: 1798 bits (4657), Expect = 0.0
Identity = 867/909 (95.38%), Postives = 874/909 (96.15%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQ 60
           MNQHSFP MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRI+CSASEHSQ
Sbjct: 1   MNQHSFPPMNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIICSASEHSQ 60

Query: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDR 120
           HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQ AHFLRERYKSLKDLKDEIYDR
Sbjct: 61  HAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQLAHFLRERYKSLKDLKDEIYDR 120

Query: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHD 180
           HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA+IGDFN WSPRE+VAREGHLGHD
Sbjct: 121 HANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVIGDFNGWSPREHVAREGHLGHD 180

Query: 181 DYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240
           DYGYWFIILEDKLK GEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR
Sbjct: 181 DYGYWFIILEDKLKEGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDR 240

Query: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300
           FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG
Sbjct: 241 FIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSVSYDVIDNG 300

Query: 301 KEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360
           KEYDIFNVVIDPVTREKFK KKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD
Sbjct: 301 KEYDIFNVVIDPVTREKFKAKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPD 360

Query: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420
           GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS
Sbjct: 361 GPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGS 420

Query: 421 EPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480
           EPKVSSF+YFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF
Sbjct: 421 EPKVSSFNYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDF 480

Query: 481 KRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540
           KRLVDEAHGLGLLV LDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY
Sbjct: 481 KRLVDEAHGLGLLVLLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKY 540

Query: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600
           GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL
Sbjct: 541 GDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDAL 600

Query: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660
           LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV
Sbjct: 601 LYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNV 660

Query: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720
           PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL
Sbjct: 661 PDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETL 720

Query: 721 LRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEE 780
           LRGCSLHKMIRFITFTMGG+AYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWD LEE+
Sbjct: 721 LRGCSLHKMIRFITFTMGGRAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDLLEEK 780

Query: 781 IHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTDSYES 840
           +HRDLFVFDK                           VISYIRGPFLFICNFHPTDSYES
Sbjct: 781 MHRDLFVFDKDLMGLGENEKLRTRSLPNVHHVNEATKVISYIRGPFLFICNFHPTDSYES 840

Query: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQV 882
           YSVGVEEAGEYRIILNTDETKYGGQGIIKHD YLQRTISRRIDGLRNCLEVPLPCRTAQV
Sbjct: 841 YSVGVEEAGEYRIILNTDETKYGGQGIIKHDHYLQRTISRRIDGLRNCLEVPLPCRTAQV 900

BLAST of Cp4.1LG06g08750 vs. ExPASy TrEMBL
Match: A0A6J1CCI0 (1,4-alpha-glucan branching enzyme OS=Momordica charantia OX=3673 GN=LOC111010409 PE=3 SV=1)

HSP 1 Score: 1648 bits (4267), Expect = 0.0
Identity = 800/903 (88.59%), Postives = 830/903 (91.92%), Query Frame = 0

Query: 9   MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQHAEPAKYQ 68
           MN +SS+PS KF PYK+N LLL  HSRST QSIALS KLKL I+CSASEH QHAEPAK+Q
Sbjct: 1   MNTISSTPSTKFYPYKSNFLLL-THSRSTPQSIALSGKLKLNIICSASEHPQHAEPAKHQ 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDRHANLQDLS 128
            S+K KSVSDGEK VDPVGFLTKLGISHKQFA +LRERYKSLKDLKDEI++RHANLQDLS
Sbjct: 61  QSRKPKSVSDGEKRVDPVGFLTKLGISHKQFAQYLRERYKSLKDLKDEIFNRHANLQDLS 120

Query: 129 SGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           SGFMFLGMHRHVEHRVDFMEWAPGARYCA++GDFN WS  EN AREGHLGHDDYGYWFII
Sbjct: 121 SGFMFLGMHRHVEHRVDFMEWAPGARYCAVVGDFNGWSVTENAAREGHLGHDDYGYWFII 180

Query: 189 LEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LEDKLK GEKPEELYFQQYNYVDDYDKGDSGI+IDEIFKRANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLKDGEKPEELYFQQYNYVDDYDKGDSGITIDEIFKRANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVIDNGKEYDIF 308
           PAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SY VID+ KEYDIF
Sbjct: 241 PAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSDLPSYHVIDSEKEYDIF 300

Query: 309 NVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLERI 368
           NVVIDP TREKFKVKKPPI YWLETRKGRKAWLKKY+PTIPHGSKYRVYFNTP+GPLERI
Sbjct: 301 NVVIDPKTREKFKVKKPPIAYWLETRKGRKAWLKKYSPTIPHGSKYRVYFNTPNGPLERI 360

Query: 369 PAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGSEPKVSS 428
           PAWATYVQPDA+GKQ FAIHWEPPPEYAYKWKN+ P+VPKALKIYECHVGISGSEPKVSS
Sbjct: 361 PAWATYVQPDADGKQAFAIHWEPPPEYAYKWKNSKPSVPKALKIYECHVGISGSEPKVSS 420

Query: 429 FDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLVDE 488
           F+YF EKVLPHVKEAGYNAIQL G+VEHKDYFT+GYRVTNFFAVSSRYGTPEDFKRLVDE
Sbjct: 421 FNYFIEKVLPHVKEAGYNAIQLIGLVEHKDYFTVGYRVTNFFAVSSRYGTPEDFKRLVDE 480

Query: 489 AHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVL 548
           AHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVL
Sbjct: 481 AHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQDVL 540

Query: 549 HFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLMLA 608
           HFLLSNLNWWVVEY IDGF FHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYL+LA
Sbjct: 541 HFLLSNLNWWVVEYGIDGFHFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLILA 600

Query: 609 NEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDHEWS 668
           NEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFL+NVPDHEWS
Sbjct: 601 NEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLENVPDHEWS 660

Query: 669 MNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETLLRGCSL 728
           MNKIVSSL DNRHY NKMLLYAENH+QSISGGRSYAEILFGD KEHG GSKETLLRGCSL
Sbjct: 661 MNKIVSSLTDNRHYANKMLLYAENHNQSISGGRSYAEILFGDIKEHGPGSKETLLRGCSL 720

Query: 729 HKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEEIHRDLF 788
           HKMIR ITFT+GG AYLNFMGNEFGHPKRVEFPMPSNN SFSLANRQWD LE+E+HRDLF
Sbjct: 721 HKMIRTITFTIGGCAYLNFMGNEFGHPKRVEFPMPSNNFSFSLANRQWDLLEKEVHRDLF 780

Query: 789 VFDK---------------------------VISYIRGPFLFICNFHPTDSYESYSVGVE 848
           VFDK                           VISYIRGPFLFI NFHPTDSYE YSVGVE
Sbjct: 781 VFDKDLMGLDENERLLTRSLPNIHHINETTKVISYIRGPFLFIFNFHPTDSYERYSVGVE 840

Query: 849 EAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLSRI 882
           EAGEYRIILNTDE KYGG+GIIKHD YLQRTIS+RIDGLRNCLEV LP RTAQVYKLSRI
Sbjct: 841 EAGEYRIILNTDEIKYGGRGIIKHDHYLQRTISQRIDGLRNCLEVSLPSRTAQVYKLSRI 900

BLAST of Cp4.1LG06g08750 vs. ExPASy TrEMBL
Match: A0A1S3BIP3 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 1647 bits (4265), Expect = 0.0
Identity = 801/913 (87.73%), Postives = 833/913 (91.24%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPS--IKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEH 60
           MN    P MNALS SPS  IKF  YKT H LLLP  RSTLQSI+LS KLKL+I CSASEH
Sbjct: 1   MNHQHSPTMNALSHSPSPSIKFSSYKT-HSLLLPQPRSTLQSISLSGKLKLKISCSASEH 60

Query: 61  SQHAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIY 120
            +HA P K+Q SKK K+VSDGEKGVDPVGFLTKLGISHKQF+ +LRERYKSLKDLK+EI+
Sbjct: 61  PEHAAPRKHQPSKKAKNVSDGEKGVDPVGFLTKLGISHKQFSQYLRERYKSLKDLKEEIF 120

Query: 121 DRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLG 180
           +RHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA++GDFN WSP EN AREGH G
Sbjct: 121 NRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVVGDFNGWSPTENAAREGHFG 180

Query: 181 HDDYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGE 240
           HDDYGYWFIILEDKLK GEKP+ELYFQQYNYVDDYDKGDSG+SIDEIFKRANDEYWEPGE
Sbjct: 181 HDDYGYWFIILEDKLKEGEKPDELYFQQYNYVDDYDKGDSGVSIDEIFKRANDEYWEPGE 240

Query: 241 DRFIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDV 300
           DRFIKNRFEVPAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SYDV
Sbjct: 241 DRFIKNRFEVPAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSNLPSYDV 300

Query: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYF 360
           IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKY+PTIPHGSKYRVYF
Sbjct: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYSPTIPHGSKYRVYF 360

Query: 361 NTPDGPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVG 420
           NTP+GPLERIPAWATYVQPDA GKQGFAIHWEPPPEYAYKWKNT PNVPKALKIYECHVG
Sbjct: 361 NTPNGPLERIPAWATYVQPDAHGKQGFAIHWEPPPEYAYKWKNTKPNVPKALKIYECHVG 420

Query: 421 ISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGT 480
           ISGSEPKVSSF+YF EKVLPHVKEAGYNAIQLFGVVEHKDYFT+GYRVTNFFAVSSRYGT
Sbjct: 421 ISGSEPKVSSFNYFIEKVLPHVKEAGYNAIQLFGVVEHKDYFTVGYRVTNFFAVSSRYGT 480

Query: 481 PEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540
           PEDFKRLVDEAHGLGLLVFLDIVHSYA+ADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR
Sbjct: 481 PEDFKRLVDEAHGLGLLVFLDIVHSYASADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540

Query: 541 MFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600
           MFKYGDQDVLHFLLSNLNWWVVEY IDGF FHSLSSMIYTHNGFASFTGDMEEFCNQYVD
Sbjct: 541 MFKYGDQDVLHFLLSNLNWWVVEYHIDGFLFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600

Query: 601 KDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSF 660
           KDALLYL+LANEILH LHP+IITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMWSSF
Sbjct: 601 KDALLYLILANEILHALHPDIITIAEDATYYPGLCEPISQGGLGFDYYVNLSASEMWSSF 660

Query: 661 LQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGS 720
           LQNVPD EW+MNKIVSSLI NRH  NKMLL+AENHSQSISGGRSYAEILFGD KEHGAGS
Sbjct: 661 LQNVPDQEWNMNKIVSSLIGNRHSTNKMLLFAENHSQSISGGRSYAEILFGDIKEHGAGS 720

Query: 721 KETLLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDF 780
           KETLLRGCSLHKMIR ITFT+GG+AYLNFMGNEFGHPKRVEFPM SNN SFSLANRQWD 
Sbjct: 721 KETLLRGCSLHKMIRLITFTIGGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDL 780

Query: 781 LEEEIHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTD 840
           LE+E+H  LF+FDK                           VISYIRGPFLFI NFHPTD
Sbjct: 781 LEKEMHHGLFLFDKELMGLDENEKILTRSLPNVHHVNETTKVISYIRGPFLFIYNFHPTD 840

Query: 841 SYESYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCR 882
           S+E YSVGVEEAGEYRI+LNTDE +YGGQG IKHDQYLQRTISRRIDGLRNCLEV LPCR
Sbjct: 841 SFERYSVGVEEAGEYRIMLNTDEIEYGGQGNIKHDQYLQRTISRRIDGLRNCLEVSLPCR 900

BLAST of Cp4.1LG06g08750 vs. ExPASy TrEMBL
Match: A0A1S4DX00 (1,4-alpha-glucan branching enzyme OS=Cucumis melo OX=3656 GN=LOC103490131 PE=3 SV=1)

HSP 1 Score: 1630 bits (4220), Expect = 0.0
Identity = 791/903 (87.60%), Postives = 823/903 (91.14%), Query Frame = 0

Query: 1   MNQHSFPAMNALSSSPS--IKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEH 60
           MN    P MNALS SPS  IKF  YKT H LLLP  RSTLQSI+LS KLKL+I CSASEH
Sbjct: 1   MNHQHSPTMNALSHSPSPSIKFSSYKT-HSLLLPQPRSTLQSISLSGKLKLKISCSASEH 60

Query: 61  SQHAEPAKYQHSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIY 120
            +HA P K+Q SKK K+VSDGEKGVDPVGFLTKLGISHKQF+ +LRERYKSLKDLK+EI+
Sbjct: 61  PEHAAPRKHQPSKKAKNVSDGEKGVDPVGFLTKLGISHKQFSQYLRERYKSLKDLKEEIF 120

Query: 121 DRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLG 180
           +RHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCA++GDFN WSP EN AREGH G
Sbjct: 121 NRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCAVVGDFNGWSPTENAAREGHFG 180

Query: 181 HDDYGYWFIILEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGE 240
           HDDYGYWFIILEDKLK GEKP+ELYFQQYNYVDDYDKGDSG+SIDEIFKRANDEYWEPGE
Sbjct: 181 HDDYGYWFIILEDKLKEGEKPDELYFQQYNYVDDYDKGDSGVSIDEIFKRANDEYWEPGE 240

Query: 241 DRFIKNRFEVPAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDV 300
           DRFIKNRFEVPAKLYE+LFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS   SYDV
Sbjct: 241 DRFIKNRFEVPAKLYEQLFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSSNLPSYDV 300

Query: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYF 360
           IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKY+PTIPHGSKYRVYF
Sbjct: 301 IDNGKEYDIFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYSPTIPHGSKYRVYF 360

Query: 361 NTPDGPLERIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVG 420
           NTP+GPLERIPAWATYVQPDA GKQGFAIHWEPPPEYAYKWKNT PNVPKALKIYECHVG
Sbjct: 361 NTPNGPLERIPAWATYVQPDAHGKQGFAIHWEPPPEYAYKWKNTKPNVPKALKIYECHVG 420

Query: 421 ISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGT 480
           ISGSEPKVSSF+YF EKVLPHVKEAGYNAIQLFGVVEHKDYFT+GYRVTNFFAVSSRYGT
Sbjct: 421 ISGSEPKVSSFNYFIEKVLPHVKEAGYNAIQLFGVVEHKDYFTVGYRVTNFFAVSSRYGT 480

Query: 481 PEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540
           PEDFKRLVDEAHGLGLLVFLDIVHSYA+ADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR
Sbjct: 481 PEDFKRLVDEAHGLGLLVFLDIVHSYASADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTR 540

Query: 541 MFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600
           MFKYGDQDVLHFLLSNLNWWVVEY IDGF FHSLSSMIYTHNGFASFTGDMEEFCNQYVD
Sbjct: 541 MFKYGDQDVLHFLLSNLNWWVVEYHIDGFLFHSLSSMIYTHNGFASFTGDMEEFCNQYVD 600

Query: 601 KDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSF 660
           KDALLYL+LANEILH LHP+IITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMWSSF
Sbjct: 601 KDALLYLILANEILHALHPDIITIAEDATYYPGLCEPISQGGLGFDYYVNLSASEMWSSF 660

Query: 661 LQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGS 720
           LQNVPD EW+MNKIVSSLI NRH  NKMLL+AENHSQSISGGRSYAEILFGD KEHGAGS
Sbjct: 661 LQNVPDQEWNMNKIVSSLIGNRHSTNKMLLFAENHSQSISGGRSYAEILFGDIKEHGAGS 720

Query: 721 KETLLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDF 780
           KETLLRGCSLHKMIR ITFT+GG+AYLNFMGNEFGHPKRVEFPM SNN SFSLANRQWD 
Sbjct: 721 KETLLRGCSLHKMIRLITFTIGGRAYLNFMGNEFGHPKRVEFPMLSNNFSFSLANRQWDL 780

Query: 781 LEEEIHRDLFVFDK---------------------------VISYIRGPFLFICNFHPTD 840
           LE+E+H  LF+FDK                           VISYIRGPFLFI NFHPTD
Sbjct: 781 LEKEMHHGLFLFDKELMGLDENEKILTRSLPNVHHVNETTKVISYIRGPFLFIYNFHPTD 840

Query: 841 SYESYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCR 872
           S+E YSVGVEEAGEYRI+LNTDE +YGGQG IKHDQYLQRTISRRIDGLRNCLEV LPCR
Sbjct: 841 SFERYSVGVEEAGEYRIMLNTDEIEYGGQGNIKHDQYLQRTISRRIDGLRNCLEVSLPCR 900

BLAST of Cp4.1LG06g08750 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 645/905 (71.27%), Postives = 737/905 (81.44%), Query Frame = 0

Query: 9   MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KL+I C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDRHANLQDLS 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER+K+LKDLKDEI+ RH + +D +
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           SGF  LGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LEDKL+ GE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGSEPKV 428
           R+PAWATYVQP+ EGKQ +AIHWEP PE AYKWK + P VP++L+IYECHVGISGSEPKV
Sbjct: 361 RVPAWATYVQPEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISGSEPKV 420

Query: 429 SSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLV 488
           S+F+ F +KVLPHVK AGYNAIQL GV EHKDYFT+GYRVTNFFA SSRYGTP+DFKRLV
Sbjct: 421 STFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDDFKRLV 480

Query: 489 DEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQD 548
           DEAHGLGLLVFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFKYGD D
Sbjct: 481 DEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLD 540

Query: 549 VLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLM 608
           VLHFL+SNLNWW+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DAL+YL+
Sbjct: 541 VLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDALMYLI 600

Query: 609 LANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDHE 668
           LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L NVPD+E
Sbjct: 601 LANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNE 660

Query: 669 WSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETLLRGC 728
           WSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE L RG 
Sbjct: 661 WSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRGI 720

Query: 729 SLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEEIHRD 788
           SLHKMIR ITFT GG+AYLNFMGNEFGHP+RVEFP  SNN SFSLANR+WD LE  +H  
Sbjct: 721 SLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHH 780

Query: 789 LFVFDK---------------------------VISYIRGPFLFICNFHPTDSYESYSVG 848
           LF FDK                           VIS+ RGPFLFI NFHP++SYE Y VG
Sbjct: 781 LFSFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVG 840

Query: 849 VEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLS 883
           VEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLEV LP RTAQVYKL+
Sbjct: 841 VEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLEVFLPSRTAQVYKLT 899

BLAST of Cp4.1LG06g08750 vs. TAIR 10
Match: AT3G20440.3 (Alpha amylase family protein )

HSP 1 Score: 1326.6 bits (3432), Expect = 0.0e+00
Identity = 638/912 (69.96%), Postives = 730/912 (80.04%), Query Frame = 0

Query: 9   MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KL+I C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDRHANLQDLS 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER+K+LKDLKDEI+ RH + +D +
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           SGF  LGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LEDKL+ GE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQP-----DAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISG 428
           R+PAWATYVQP     + EGKQ +AIHWEP PE AYKWK + P VP++L+IYECHVGISG
Sbjct: 361 RVPAWATYVQPGMTAFEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISG 420

Query: 429 SEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPED 488
           SEPKVS+F+ F +KVLPHVK AGYNAIQL GV EHKDYFT+GYRVTNFFA SSRYGTP+D
Sbjct: 421 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDD 480

Query: 489 FKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFK 548
           FKRLVDEAHGLGLLVFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFK
Sbjct: 481 FKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFK 540

Query: 549 YGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDA 608
           YGD DVLHFL+SNLNWW+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DA
Sbjct: 541 YGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDA 600

Query: 609 LLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQN 668
           L+YL+LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L N
Sbjct: 601 LMYLILANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDN 660

Query: 669 VPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKET 728
           VPD+EWSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE 
Sbjct: 661 VPDNEWSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKEL 720

Query: 729 LLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEE 788
           L RG SLHKMIR ITFT GG+AYLNFMGNEFGHP+RVEFP  SNN SFSLANR+WD LE 
Sbjct: 721 LDRGISLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLES 780

Query: 789 EIHRDLFVFDK-----------------------------VISYIRGPFLFICNFHPTDS 848
            +H  LF FDK                             VIS+ RGPFLFI NFHP++S
Sbjct: 781 GVHHHLFSFDKVSELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNS 840

Query: 849 YESYSVGVEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRT 883
           YE Y VGVEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLE       
Sbjct: 841 YEKYDVGVEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLE------- 897

BLAST of Cp4.1LG06g08750 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 1282.3 bits (3317), Expect = 0.0e+00
Identity = 619/905 (68.40%), Postives = 710/905 (78.45%), Query Frame = 0

Query: 9   MNALSSSPSIKFPPYKTNHLLLLPHSRSTLQSIALSRKLKLRIVCSASEHSQHAEPAKYQ 68
           M +LS+     F P   N+L++    R  +  +   RK+KL+I C A+E  +  +  K  
Sbjct: 1   MVSLSNQTRFSFHP---NNLVVSEKRRLGISGVNFPRKIKLKITCFAAERPRQEKQKKKS 60

Query: 69  HSKKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEIYDRHANLQDLS 128
            S+ T   SD E GVDPVGFLT+LGI+ + FA FLRER+K+LKDLKDEI+ RH + +D +
Sbjct: 61  QSQST---SDAEAGVDPVGFLTRLGIADRIFAQFLRERHKALKDLKDEIFKRHFDFRDFA 120

Query: 129 SGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHLGHDDYGYWFII 188
           SGF  LGMHRH+EHRVDFM+W PG+RY A+IGDFN WSP EN AREG  GHDDYGYWFII
Sbjct: 121 SGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLFGHDDYGYWFII 180

Query: 189 LEDKLKGGEKPEELYFQQYNYVDDYDKGDSGISIDEIFKRANDEYWEPGEDRFIKNRFEV 248
           LEDKL+ GE+P+ELYFQQYNYVDDYDKGDSG+S +EIF++ANDEYWEPGEDRFIKNRFEV
Sbjct: 181 LEDKLREGEEPDELYFQQYNYVDDYDKGDSGVSAEEIFQKANDEYWEPGEDRFIKNRFEV 240

Query: 249 PAKLYEELFGPNGPQTLEELEEIPDAETRYKAWKEQHKDDPSS--VSYDVID--NGKEYD 308
           PAKLYE++FGPN PQTLEEL +IPDAETRYK WKE+HKDDP S     D+ID   GK YD
Sbjct: 241 PAKLYEQMFGPNSPQTLEELGDIPDAETRYKQWKEEHKDDPPSNLPPCDIIDKGQGKPYD 300

Query: 309 IFNVVIDPVTREKFKVKKPPIPYWLETRKGRKAWLKKYNPTIPHGSKYRVYFNTPDGPLE 368
           IFNVV  P   +KF  K+PPIPYWLETRKGRKAWL+KY P +PHGSKYR+YFNTPDGPLE
Sbjct: 301 IFNVVTSPEWTKKFYEKEPPIPYWLETRKGRKAWLQKYIPAVPHGSKYRLYFNTPDGPLE 360

Query: 369 RIPAWATYVQPDAEGKQGFAIHWEPPPEYAYKWKNTSPNVPKALKIYECHVGISGSEPKV 428
           R+PAWATYVQP+ EGKQ +AIHWEP PE AYKWK + P VP++L+IYECHVGISGSEPKV
Sbjct: 361 RVPAWATYVQPEDEGKQAYAIHWEPSPEAAYKWKYSKPKVPESLRIYECHVGISGSEPKV 420

Query: 429 SSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLGYRVTNFFAVSSRYGTPEDFKRLV 488
           S+F+ F +K                              VTNFFA SSRYGTP+DFKRLV
Sbjct: 421 STFEEFTKK------------------------------VTNFFAASSRYGTPDDFKRLV 480

Query: 489 DEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCYFHTGKRGHHKYWGTRMFKYGDQD 548
           DEAHGLGLLVFLDIVHSYAAAD+MVGLS FDGSNDCYFH GKRGHHK+WGTRMFKYGD D
Sbjct: 481 DEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLD 540

Query: 549 VLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFASFTGDMEEFCNQYVDKDALLYLM 608
           VLHFL+SNLNWW+ EY++DG++FHSL+SMIYTHNGFASF  D++++CNQYVD+DAL+YL+
Sbjct: 541 VLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFASFNNDLDDYCNQYVDRDALMYLI 600

Query: 609 LANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLGFDYYVNLSASEMWSSFLQNVPDHE 668
           LANEILH  HPNIITIAEDATYYPGLCEP SQGGLGFDYYVNLSASEMW S L NVPD+E
Sbjct: 601 LANEILHVQHPNIITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNE 660

Query: 669 WSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRSYAEILFGDTKEHGAGSKETLLRGC 728
           WSM+KIVS+L+ N+ Y +KML YAENH+QSISGGRS+AEILFG       G KE L RG 
Sbjct: 661 WSMSKIVSTLVANKEYADKMLSYAENHNQSISGGRSFAEILFGGVDNGSPGGKELLDRGI 720

Query: 729 SLHKMIRFITFTMGGQAYLNFMGNEFGHPKRVEFPMPSNNLSFSLANRQWDFLEEEIHRD 788
           SLHKMIR ITFT GG+AYLNFMGNEFGHP+RVEFP  SNN SFSLANR+WD LE  +H  
Sbjct: 721 SLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANRRWDLLESGVHHH 780

Query: 789 LFVFDK---------------------------VISYIRGPFLFICNFHPTDSYESYSVG 848
           LF FDK                           VIS+ RGPFLFI NFHP++SYE Y VG
Sbjct: 781 LFSFDKELMDLDKSKGILSRGLPSIHHVNDANMVISFSRGPFLFIFNFHPSNSYEKYDVG 840

Query: 849 VEEAGEYRIILNTDETKYGGQGIIKHDQYLQRTISRRIDGLRNCLEVPLPCRTAQVYKLS 883
           VEEAGEY +ILN+DE KYGGQGI+  D YLQR+IS+RIDG RNCLEV LP RTAQVYKL+
Sbjct: 841 VEEAGEYTMILNSDEVKYGGQGIVTEDHYLQRSISKRIDGQRNCLEVFLPSRTAQVYKLT 869

BLAST of Cp4.1LG06g08750 vs. TAIR 10
Match: AT2G36390.1 (starch branching enzyme 2.1 )

HSP 1 Score: 451.4 bits (1160), Expect = 1.6e-126
Identity = 233/579 (40.24%), Postives = 344/579 (59.41%), Query Frame = 0

Query: 343 NPTIPHGSKYRVYFNTPDGPLERIPAWATY-VQPDAEGKQGFAIHWEPPPEYAYKWKNTS 402
           +P IPHGS+ ++  +TP G  + IPAW  Y VQP  E      ++++PP E  Y +K+  
Sbjct: 261 SPAIPHGSRVKIRMDTPSGIKDSIPAWIKYSVQPPGEIPYN-GVYYDPPEEDKYAFKHPR 320

Query: 403 PNVPKALKIYECHVGISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLG 462
           P  P +L+IYE HVG+S +EPK++++  FR+ VLP +K+ GYNA+Q+  + EH  Y + G
Sbjct: 321 PKKPTSLRIYESHVGMSSTEPKINTYANFRDDVLPRIKKLGYNAVQIMAIQEHAYYASFG 380

Query: 463 YRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCY 522
           Y VTNFFA SSR+GTP+D K L+D+AH LGL+V +DIVHS+A+ + + GL  FDG++  Y
Sbjct: 381 YHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHASKNTLDGLDMFDGTDGQY 440

Query: 523 FHTGKRGHHKYWGTRMFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGF- 582
           FH+G RG+H  W +R+F YG  +VL +LLSN  WW+ EY+ DGFRF  ++SM+YTH+G  
Sbjct: 441 FHSGSRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQ 500

Query: 583 ASFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLG 642
             FTG+  E+     D DA++YLML N+++HGL+P  I + ED +  P  C P   GG+G
Sbjct: 501 VEFTGNYNEYFGYSTDVDAVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFCVPVEDGGVG 560

Query: 643 FDYYVNLSASEMWSSFLQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRS 702
           FDY ++++ ++ W   L+   D +W +  I  +L  NR +  K ++YAE+H Q++ G ++
Sbjct: 561 FDYRLHMAVADKWIELLKK-RDEDWQVGDITFTL-TNRRWGEKCVVYAESHDQALVGDKT 620

Query: 703 YAEILFG-DTKEHGAGSKET---LLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRV 762
            A  L   D  +  A  ++    + RG +LHKMIR IT  +GG+ YLNFMGNEFGHP+ +
Sbjct: 621 IAFWLMDKDMYDFMAVDRQATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWI 680

Query: 763 EFP-----------MPSNNLSFSLANRQWDFLEEEIHR---------------DLFVF-- 822
           +FP           +  NN S+    R++D  + E  R               + + F  
Sbjct: 681 DFPRTDQHLPDGRVIAGNNGSYDKCRRRFDLGDAEYLRYHGLQEFDRAMQNLEETYGFMT 740

Query: 823 ------------DKVISYIRGPFLFICNFHPTDSYESYSVGVEEAGEYRIILNTDETKYG 875
                       D+VI + RG  LF+ NFH T+SY  Y +G    G+Y+I+L++D + +G
Sbjct: 741 SEHQYISRKDEGDRVIVFERGNLLFVFNFHWTNSYSDYRIGCSVPGKYKIVLDSDNSLFG 800

HSP 2 Score: 62.0 bits (149), Expect = 2.8e-09
Identity = 42/138 (30.43%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 65  AKYQHS-------KKTKSVSDGEKGVDPVGFLTKLGISHKQFAHFLRERYKSLKDLKDEI 124
           AK  HS       +K     DG++  D    L     SH+   + L  RY   + L++EI
Sbjct: 136 AKMSHSVDQEVGQRKIPPPGDGKRIYDIDPMLN----SHR---NHLDYRYGQYRKLREEI 195

Query: 125 YDRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYCALIGDFNDWSPRENVAREGHL 184
                 L+  S G+   G  R     + + EWAPGA+  +LIGDFN+W+ + +V     +
Sbjct: 196 DKNEGGLEAFSRGYEIFGFTRSAT-GITYREWAPGAKAASLIGDFNNWNAKSDV-----M 255

Query: 185 GHDDYGYWFIILEDKLKG 196
             +D+G W I L +   G
Sbjct: 256 ARNDFGVWEIFLPNNADG 260

BLAST of Cp4.1LG06g08750 vs. TAIR 10
Match: AT5G03650.1 (starch branching enzyme 2.2 )

HSP 1 Score: 450.7 bits (1158), Expect = 2.8e-126
Identity = 238/586 (40.61%), Postives = 347/586 (59.22%), Query Frame = 0

Query: 343 NPTIPHGSKYRVYFNTPDGPLERIPAWATYVQPDAEGKQGF-AIHWEPPPEYAYKWKNTS 402
           +P IPHGS+ ++  +TP G  + IPAW  +    A G+  F  I+++PP E  Y +K+  
Sbjct: 226 SPAIPHGSRVKIRMDTPSGIKDSIPAWIKF-SVQAPGEIPFNGIYYDPPEEEKYVFKHPQ 285

Query: 403 PNVPKALKIYECHVGISGSEPKVSSFDYFREKVLPHVKEAGYNAIQLFGVVEHKDYFTLG 462
           P  PK+L+IYE HVG+S +EP V+++  FR+ VLP +K+ GYNA+Q+  + EH  Y + G
Sbjct: 286 PKRPKSLRIYEAHVGMSSTEPMVNTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFG 345

Query: 463 YRVTNFFAVSSRYGTPEDFKRLVDEAHGLGLLVFLDIVHSYAAADEMVGLSSFDGSNDCY 522
           Y VTNFFA SSR GTPE+ K L+D AH LGL+V +DIVHS+A+ + + GL+ FDG++  Y
Sbjct: 346 YHVTNFFAPSSRCGTPEELKSLIDRAHELGLVVLMDIVHSHASKNTLDGLNMFDGTDAHY 405

Query: 523 FHTGKRGHHKYWGTRMFKYGDQDVLHFLLSNLNWWVVEYRIDGFRFHSLSSMIYTHNGFA 582
           FH+G RG+H  W +R+F YG  +VL +LLSN  WW+ EY+ DGFRF  ++SM+YTH+G +
Sbjct: 406 FHSGPRGYHWMWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLS 465

Query: 583 -SFTGDMEEFCNQYVDKDALLYLMLANEILHGLHPNIITIAEDATYYPGLCEPTSQGGLG 642
             FTG+  E+     D DA+ YLML N+++HGL+P  IT+ ED +  P  C P   GG+G
Sbjct: 466 VGFTGNYTEYFGLETDVDAVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFCIPVQDGGVG 525

Query: 643 FDYYVNLSASEMWSSFLQNVPDHEWSMNKIVSSLIDNRHYVNKMLLYAENHSQSISGGRS 702
           FDY ++++ ++ W   L+   D +W M  I+ +L  NR +  K + YAE+H Q++ G ++
Sbjct: 526 FDYRLHMAIADKWIEMLKK-RDEDWQMGDIIYTL-TNRRWSEKCISYAESHDQALVGDKT 585

Query: 703 YAEILFG----DTKEHGAGSKETLLRGCSLHKMIRFITFTMGGQAYLNFMGNEFGHPKRV 762
            A  L      D       S   + RG +LHKMIR IT  +GG+ YLNFMGNEFGHP+ +
Sbjct: 586 IAFWLMDKDMYDFMAVDRPSTPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWI 645

Query: 763 EFP-----------MPSNNLSFSLANRQWDFLEEEI--HRDLFVF--------------- 822
           +FP           +P NN S+    R++D  + +   +R L  F               
Sbjct: 646 DFPRGEQRLSDGSVIPGNNFSYDKCRRRFDLGDADYLRYRGLQEFDQAMQHLEENYGFMT 705

Query: 823 ------------DKVISYIRGPFLFICNFHPTDSYESYSVGVEEAGEYRIILNTDETKYG 878
                       D+VI + RG  +F+ NFH T SY  Y +G  + G+Y+I+L++D+  +G
Sbjct: 706 SEHQFISRKDEADRVIVFERGDLVFVFNFHWTSSYFDYRIGCSKPGKYKIVLDSDDPLFG 765

HSP 2 Score: 60.1 bits (144), Expect = 1.1e-08
Identity = 31/99 (31.31%), Postives = 52/99 (52.53%), Query Frame = 0

Query: 97  KQFAHFLRERYKSLKDLKDEIYDRHANLQDLSSGFMFLGMHRHVEHRVDFMEWAPGARYC 156
           + + + L  RY   K L++EI      L+  S G+  LG  R  +  + + EWAPGA+  
Sbjct: 133 RTYNNHLDYRYGQYKRLREEIDKYEGGLEAFSRGYEKLGFSRS-DAGITYREWAPGAKAA 192

Query: 157 ALIGDFNDWSPRENVAREGHLGHDDYGYWFIILEDKLKG 196
           +LIGDFN+W+   ++     +  +++G W I L +   G
Sbjct: 193 SLIGDFNNWNSNADI-----MTRNEFGVWEIFLPNNTDG 225