Cp4.1LG06g07750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG06g07750 vs. ExPASy Swiss-Prot
Match: G0S8G9 (Eukaryotic translation initiation factor 5B OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) OX=759272 GN=CTHT_0029840 PE=1 SV=2)

HSP 1 Score: 396.0 bits (1016), Expect = 1.3e-108
Identity = 325/741 (43.86%), Postives = 430/741 (58.03%), Query Frame = 0

Query: 348  KNKRKKKKSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEA--------------- 407
            KNK K+KK G +   ED LD    E  EG A +   +P LS+++A               
Sbjct: 58   KNKEKRKKKGLS---EDWLD---GETPEGQAPAAAPEPDLSAKQAEEANLEDEWALPDKK 117

Query: 408  -----KVESQPKSQPEVVAPPEKEVEEESTESAAARKK-KKKKEKEKEKKAAAAAAAAAE 467
                 K +   K Q E     ++EV E    + A +++ KK++EK+++K+ AA   AA  
Sbjct: 118  GKKGGKQQQNKKQQQEEKKKDDEEVPEIRVLTKAEKERLKKEREKQRKKEQAAKKKAAGP 177

Query: 468  GNDEKIEEVKTEIIEPK------------KGAAKGKVVEKKVPKHVR---EMQEAMARRK 527
               +K E  K    E K            + AA G   +KK+P H+R   + QE + RR+
Sbjct: 178  AQPQKAEPAKPAEEEKKPEEPAPAPAPAPEPAAGGS--KKKIPAHLRLIQKQQEELRRRQ 237

Query: 528  EEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRKKQEGKLLTGKQ 587
            EEE+RR   EEE+ R EEEERR +EE +R+ EE K RKK++EKEK+ + K+EGK LT  Q
Sbjct: 238  EEEQRRL--EEERRRIEEEERRAEEERKRK-EEEKARKKQKEKEKIEQLKREGKYLTKAQ 297

Query: 588  KEE----QRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQTNGSAQTK 647
            +EE    QR LE MR   +  A     +  + +A  ++ K + KK +          Q  
Sbjct: 298  REEKARNQRMLEQMRAAGIKIAALEEKNKEEGAAEGEKEKKEKKKPEKKRRPNKVDEQKA 357

Query: 648  VVEHVEERIQEKDVAETEILESEKIEAVESTLEEE-KSDTIEATVDNEIQEDEDDDEWDA 707
            + E  E   Q+ + A  E  E  ++E  ++  EE+ K    E +VD + +   + D+ D 
Sbjct: 358  LEEAAERARQQAEAAAREAEEKARLEREKAEAEEKAKQAAAEESVDEDWEAAAESDKEDV 417

Query: 708  -KSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASP-AQKSLPSQ 767
              SWD A  D   K    +EE E E     K ++K    T     A+P  P A+K    +
Sbjct: 418  PDSWDAAASD-EEKEEEEEEEEEEEKPAPKKTEKKPEPKTEEKKKAEPKKPEAKKEEVKK 477

Query: 768  PIKSQD----IENTKKQHEVEVVDKGKGKD-----DAVKKKTSIPDATPKQQEENLRSPI 827
            P   ++     E   K     +  + K ++     +A KK+ +     P   ++NLRSPI
Sbjct: 478  PEPKKEEPKKAEANGKPATTTLPTRSKEEEKPKAAEAEKKEVAARPKKPVVNKDNLRSPI 537

Query: 828  CCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAK--L 887
            CCI+GHVDTGKTKLLD IR TNVQEGEAGGITQQIGATYFP E I+++T  +  D K   
Sbjct: 538  CCILGHVDTGKTKLLDKIRQTNVQEGEAGGITQQIGATYFPVEAIKQKTAVVNKDGKFEF 597

Query: 888  KVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFII 947
            KVPGLLIIDTPGHESF+NLRSRGS LC++AILVVDIMHGLEPQTIESL LLR R T F++
Sbjct: 598  KVPGLLIIDTPGHESFSNLRSRGSSLCNIAILVVDIMHGLEPQTIESLRLLRERKTPFVV 657

Query: 948  ALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKE 1007
            ALNK+DRLYGWK I N    ++   Q+K VQNEF  RL Q+  QF EQG N+EL+Y+NK 
Sbjct: 658  ALNKIDRLYGWKKIENNGFRESFALQNKAVQNEFRNRLDQVKLQFAEQGFNSELFYENKN 717

Query: 1008 MGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTI 1035
                 S+VPTSA TGEGIPDML L+VQ  Q+ M   L Y  E+Q TVLEVK +EG G TI
Sbjct: 718  FARYVSLVPTSAHTGEGIPDMLKLIVQLCQERMASSLMYLSELQATVLEVKAIEGFGVTI 777

BLAST of Cp4.1LG06g07750 vs. ExPASy Swiss-Prot
Match: P39730 (Eukaryotic translation initiation factor 5B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FUN12 PE=1 SV=2)

HSP 1 Score: 340.1 bits (871), Expect = 8.7e-92
Identity = 294/752 (39.10%), Postives = 409/752 (54.39%), Query Frame = 0

Query: 299  KKSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGR 358
            KKS K + +       ++ A  +EI  T + N +S +    T   A +S           
Sbjct: 3    KKSKKNQQNYWDEEFEEDAAQNEEISATPTPNPESSAGADDTSREASASAEG-------- 62

Query: 359  TAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVE----- 418
                        AE  EG   S         ++  +E +   +P + +  EKE E     
Sbjct: 63   ------------AEAIEGDFMSTLKQSKKKQEKKVIEEKKDGKPILKSKKEKEKEKKEKE 122

Query: 419  -EESTESAAARKKKKKKEKEKEK---KAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKG 478
             ++  E AA +K +++ +KEK K   K     AAA +   EK ++ K E  +P   +A  
Sbjct: 123  KQKKKEQAARKKAQQQAQKEKNKELNKQNVEKAAAEKAAAEKSQKSKGESDKP---SASA 182

Query: 479  KVVEKKVPKHVREMQEAMARRK--EEEERRKREEEEKLRKEEEERRRQEELERQAEEAKR 538
            K   KKVP  +  ++  +  +K  EE+E+ +REEEE+L KEEEER   E  E+  EEAK 
Sbjct: 183  KKPAKKVPAGLAALRRQLELKKQLEEQEKLEREEEERLEKEEEERLANE--EKMKEEAKA 242

Query: 539  RKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSN----AGGLPLSTSDPSAPA 598
             KKE+EK K  ++K EGKLLT KQKEE++ LE  R  +LS+      GL     + + P 
Sbjct: 243  AKKEKEKAKREKRKAEGKLLTRKQKEEKKLLERRRAALLSSGNVKVAGLAKKDGEENKP- 302

Query: 599  KRPKYQTKKTKPAHHQTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEK 658
            K+  Y  KK +               E+  E I+     ++E++  +++        +E 
Sbjct: 303  KKVVYSKKKKRTTQ------------ENASEAIKSDSKKDSEVVPDDEL--------KES 362

Query: 659  SDTIEATVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSA 718
             D +    +N    D+D++  + ++ +      +   +  +EE E E E   +++R +  
Sbjct: 363  EDVLIDDWENLALGDDDEEGTNEETQESTASHENEDQNQGEEEEEGEEEEEEEEERAHVH 422

Query: 719  PTSGGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDAT 778
              +    A  A+PA    PS                                      A+
Sbjct: 423  EVAKSTPA--ATPAATPTPS-------------------------------------SAS 482

Query: 779  PKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRER 838
            P +++  LRSPICCI+GHVDTGKTKLLD IR TNVQ GEAGGITQQIGATYFP + I+ +
Sbjct: 483  PNKKD--LRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPIDAIKAK 542

Query: 839  TRELKADAK--LKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESL 898
            T+ +    K    VPGLL+IDTPGHESF+NLRSRGS LC++AILV+DIMHGLE QTIES+
Sbjct: 543  TKVMAEYEKQTFDVPGLLVIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLEQQTIESI 602

Query: 899  NLLRMRNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQ 958
             LLR R   F++ALNK+DRLY WK I N     +  +QS+ VQ EF  R  +I  +  EQ
Sbjct: 603  KLLRDRKAPFVVALNKIDRLYDWKAIPNNSFRDSFAKQSRAVQEEFQSRYSKIQLELAEQ 662

Query: 959  GLNTELYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVL 1018
            GLN+ELY++NK M +  SIVPTSAVTGEG+PD+L LL++  QK M+K+L Y   V+ T+L
Sbjct: 663  GLNSELYFQNKNMSKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLMYLSHVEATIL 667

Query: 1019 EVKVVEGHGTTIDVILVNGVLHEGDQIVVCGM 1034
            EVKVVEG GTTIDVIL NG L EGD+IV+CGM
Sbjct: 723  EVKVVEGFGTTIDVILSNGYLREGDRIVLCGM 667

BLAST of Cp4.1LG06g07750 vs. ExPASy Swiss-Prot
Match: Q10251 (Eukaryotic translation initiation factor 5B OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC56F8.03 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 5.7e-91
Identity = 308/851 (36.19%), Postives = 447/851 (52.53%), Query Frame = 0

Query: 197  GKKKKSSQGGKKAGNAFTGFSGLDD------EDEDRNDDMDDGEDVTTITFSGKKKKSSK 256
            GKK K S       +     SG ++      +D  +ND++ +  +   ++      K  K
Sbjct: 2    GKKGKKSGYADWEDDLGEDISGQNEYLDNTSQDSPQNDELAEKSENLAVSSEKTTSKKKK 61

Query: 257  GSKKSGNLFSAALADEENDGDDSISKPDKLD-IDGVEEDDVNVISFSGKKKSSKKKSSST 316
            G K  GN     ++D+E+   +S   P +L  +  +++D+ +     GKK    KK    
Sbjct: 62   GKKNKGN--KNQVSDDESQELESPQGPKELTAVTELDDDEFDYKPKKGKKGKKSKKVEED 121

Query: 317  FTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRTAQEEDDLDKI 376
                  E+  G + L TA + LD D    K            KK K G+ AQ  ++    
Sbjct: 122  DEPQEIESPQGPKEL-TAVTELDDDEFDYKP-----------KKGKKGKKAQNNNE---- 181

Query: 377  LAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTESAAARKKKKKK 436
                      S+ A PP               PEV    +KE E E  E    R+K +KK
Sbjct: 182  ----------SEAAAPP-------------EIPEVRVKTKKEKEREKKE----REKLRKK 241

Query: 437  EKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVV--EKKVPKHVREMQEAM 496
            +++ +KK +      A  + E   EV          +AKGK     K+   +V  +Q+ +
Sbjct: 242  QQQAKKKGSTGEDTLA--SSEVSSEVDISTPAENDSSAKGKQAAGSKRKGPNVTALQKML 301

Query: 497  ARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRKKQEGKLL 556
              ++  EE  +R  EE+ R  EEE+R  E  E + EEA+ +KKE+E++K    K +GK L
Sbjct: 302  EEKRAREEEEQRIREEEARIAEEEKRLAEVEEARKEEARLKKKEKERKKKEEMKAQGKYL 361

Query: 557  TGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQTNGSAQTK 616
            + KQKE+Q   +    Q+L +  G+ ++        ++P Y  KK      ++N S  + 
Sbjct: 362  SKKQKEQQALAQRRLQQMLES--GVRVAGLSNGEKKQKPVYTNKK------KSNRSGTSS 421

Query: 617  VVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDEDDDEWDAK 676
            +       I E   A +  ++  + ++ + + + EK   +E   +NE + +   D+W+A 
Sbjct: 422  I---SSSGILESSPATSISVDEPQKDSKDDSEKVEKETEVERKEENEAEAEAVFDDWEA- 481

Query: 677  SWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQKSLPSQPIK 736
                           A EE E    N +  ++K +                       IK
Sbjct: 482  ---------------ALEEPEVAENNEVVTEKKET----------------------DIK 541

Query: 737  SQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQ---QEENLRSPICCIMGHVDT 796
            S  +E++ K  E          D    K   IP A P +    E +LRSPICCI+GHVDT
Sbjct: 542  SDAVEHSIKDKE----------DSKTDKVDDIPQAAPAESNVSESDLRSPICCILGHVDT 601

Query: 797  GKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLK--VPGLLIID 856
            GKTKLLD +R +NVQEGEAGGITQQIGATYFP E+I+++T+ +    KL+  +PGLLIID
Sbjct: 602  GKTKLLDNLRRSNVQEGEAGGITQQIGATYFPIESIKQKTKVVNKKGKLQYNIPGLLIID 661

Query: 857  TPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVDRLY 916
            TPGHESFTNLRSRG+ LC++AILV+DIMHGLEPQTIES+ LLR + T F++ALNKVDRLY
Sbjct: 662  TPGHESFTNLRSRGTSLCNIAILVIDIMHGLEPQTIESIRLLRDQKTPFVVALNKVDRLY 721

Query: 917  GWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSIVP 976
            GW +I++  I  ++ +Q K +Q EF  R+  II Q  EQGLN  LY++NK +G   S+VP
Sbjct: 722  GWHSIKDNAIQDSLSKQKKAIQREFRDRVESIILQLNEQGLNAALYFENKNLGRYVSLVP 746

Query: 977  TSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNGVL 1034
            TSA +GEG+PD++ LL+   Q  M+ ++ Y   ++CTVLEVKV+EG G TIDVIL NGVL
Sbjct: 782  TSAQSGEGVPDLVALLISLTQTRMSDRIKYITTLECTVLEVKVIEGLGATIDVILSNGVL 746

BLAST of Cp4.1LG06g07750 vs. ExPASy Swiss-Prot
Match: O60841 (Eukaryotic translation initiation factor 5B OS=Homo sapiens OX=9606 GN=EIF5B PE=1 SV=4)

HSP 1 Score: 322.8 bits (826), Expect = 1.4e-86
Identity = 355/1012 (35.08%), Postives = 516/1012 (50.99%), Query Frame = 0

Query: 61   GKKGNSKASQLKEDDDDDVDDVSEIVITG--------KKKGKSKKGGSSSAFTASSFGLL 120
            GKK  +K+    +DD D     +EI   G        K KGK KK      F        
Sbjct: 2    GKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKE 61

Query: 121  EEEGKDGANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALD 180
             EE    A     D E+V     +++EEE        KKK  +  KK  F          
Sbjct: 62   LEELSLEAQGIKADRETVAVKPTENNEEE---FTSKDKKKKGQKGKKQSFD--------- 121

Query: 181  DEKDEDVIDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDD 240
                    D+++EE++D           K S+  K A      +SG DD+D+        
Sbjct: 122  --------DNDSEELED-----------KDSKSKKTAKPKVEMYSGSDDDDD-------- 181

Query: 241  GEDVTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGV--EEDDV 300
                    F+   KK+   ++KS   +    ++E+ D    I +  +++  G   +E D 
Sbjct: 182  --------FNKLPKKAKGKAQKSNKKWDG--SEEDEDNSKKIKERSRINSSGESGDESDE 241

Query: 301  NVISFSGKKKSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSS-KAKTEAVAESSKN 360
             + S  G+KK+ K K                   N  S N D D+S K KT A  ++ K 
Sbjct: 242  FLQSRKGQKKNQKNKPGP----------------NIESGNEDDDASFKIKTVAQKKAEKK 301

Query: 361  KRKKKKSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEV---VA 420
            +R++KK      EE    + L E  E     K       SQ    E   KS+  V   V 
Sbjct: 302  ERERKK----RDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVI 361

Query: 421  PPEKEVEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGA 480
            P  +E  E  T +    +  KKK+ +K+KK             EK E+ K    E KKG 
Sbjct: 362  PASEEKAETPTAAEDDNEGDKKKKDKKKKKG------------EKEEKEK----EKKKGP 421

Query: 481  AKGKVVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAK 540
            +K           V+ MQEA+A+ KEEEER+KREEEE++++ EE   +++E ER  +E +
Sbjct: 422  SKAT---------VKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKR 481

Query: 541  RRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRP 600
             RKK++EKE+  R K+EGKLLT  Q+E + R EA     L  A G+ + + D S P KRP
Sbjct: 482  ERKKQKEKERKERLKKEGKLLTKSQREARARAEATLK--LLQAQGVEVPSKD-SLPKKRP 541

Query: 601  KYQTKKTKPAHHQTNGSAQTK------VVEHVEERIQEKDVA-------ETEILESEKIE 660
             Y+ KK K    Q      ++       VE +E+ + EK+         E E  E   ++
Sbjct: 542  IYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLD 601

Query: 661  AVESTLEEEKSDTIEATV--------DNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADE 720
              E+   +E+++ +E             E +E+E+++E D +S ++            +E
Sbjct: 602  DWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEE-----------EEE 661

Query: 721  ELESESENGMKKDRK-NSAPTSGGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVD 780
            E ESE   G ++D K +    SG  + K  S   K + S      D + TK   E    D
Sbjct: 662  EGESEGSEGDEEDEKVSDEKDSGKTLDKKPS---KEMSSDSEYDSDDDRTK---EERAYD 721

Query: 781  KGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAG 840
            K K +   ++K+    + +     E LR+PI C++GHVDTGKTK+LD +R T+VQ+GEAG
Sbjct: 722  KAKRR---IEKRRL--EHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAG 781

Query: 841  GITQQIGATYFPAENIRERTRELK--ADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDL 900
            GITQQIGAT  P E I E+T+ +K      +++PG+LIIDTPGHESF+NLR+RGS LCD+
Sbjct: 782  GITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDI 841

Query: 901  AILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKY 960
            AILVVDIMHGLEPQTIES+NLL+ +   FI+ALNK+DRLY WK   ++ +  T+K+Q K 
Sbjct: 842  AILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKN 894

Query: 961  VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWA 1020
             ++EF  R   II +F +QGLN  L+Y+NK+     S+VPTSA TG+G+  ++ LLV+  
Sbjct: 902  TKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELT 894

Query: 1021 QKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1035
            Q  ++K+L + +E++  V+EVK + G GTTIDVIL+NG L EGD I+V G++
Sbjct: 962  QTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVE 894

BLAST of Cp4.1LG06g07750 vs. ExPASy Swiss-Prot
Match: Q05D44 (Eukaryotic translation initiation factor 5B OS=Mus musculus OX=10090 GN=Eif5b PE=1 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 1.5e-83
Identity = 344/982 (35.03%), Postives = 502/982 (51.12%), Query Frame = 0

Query: 113  EEEGKDGANDDDDDDESVLTAEKD--DDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGA 172
            +++ K   +  DD D   L AE +     +E    K  GKKK  K  KK  F        
Sbjct: 4    KQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKSKGKKK--KEKKKQDFDENDILRE 63

Query: 173  LDD--------EKDEDVIDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDE 232
            L++          D D    +  E ++E   S   KKKK  +G K         +  D+ 
Sbjct: 64   LEELSLEAQGIRADRDAAAVKPTENNEEESASKQDKKKKGQKGKK---------TSFDEN 123

Query: 233  DEDRNDDMDDGEDVTT-----ITFSGKK------KKSSKGSKKSGNLFSAALADEENDGD 292
            D +  +D D     T         SG +      K S KG K   +      ++E+ D  
Sbjct: 124  DSEELEDKDSKSKKTARPNSEAPLSGSEDADDSNKLSKKGKKAQKSTKKRDGSEEDEDNS 183

Query: 293  DSISKPDKLDIDGVE--EDDVNVISFSGKKKSSKKKSSSTFTALSDENALGDEILNTASS 352
                +  +++  G    E D  + S  G+KK+ K KS  T                  S 
Sbjct: 184  KRSKERSRVNSSGESGGESDEFLQSRKGQKKNQKNKSVPT----------------VDSG 243

Query: 353  NLDSDSS-KAKTEAVAESSKNKRKKKKSG------RTAQEEDDLDKILAELGEGPATSKP 412
            N D DSS K KT A  ++ K +R+KKK        R  +E+++L+K   E          
Sbjct: 244  NEDDDSSFKIKTVAQKKAEKKEREKKKRDEEKAKLRKMKEKEELEKGKKE---------- 303

Query: 413  ADPPLSSQEAKVESQPKSQPEVV----APPEKEVEEESTESAAARK-----KKKKKEKEK 472
                   Q  + E Q + + EV+     P      EE  ++AAA +      KKKK+K+K
Sbjct: 304  -------QSKQREPQKRPEEEVLTLRGTPDTGAASEEKGDTAAALEDDNEGDKKKKDKKK 363

Query: 473  EKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKHVREMQEAMARRKEE 532
            +K              EK E+ K    E KKG +K           V+ +QEA+A+ KEE
Sbjct: 364  KK-------------TEKDEKEK----EKKKGPSKST---------VKAIQEALAKLKEE 423

Query: 533  EERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRKKQEGKLLTGKQKE 592
            EER+KREEEE++++ EE   +++E ER  +E + RKK++EKE+  R K+EGKLLT  Q+E
Sbjct: 424  EERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQRE 483

Query: 593  EQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQTNG---------SA 652
             + R E     + +    +P   S  S P KRP Y+ KK K    Q            SA
Sbjct: 484  ARARAEVTLRHLQAQGVEVP---SKDSLPKKRPVYEDKKKKKTPQQLESKEVSETLEISA 543

Query: 653  QTKVV-------EHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDT---IEATVDNE 712
              + V       E     ++ ++  +TE    +  EA+ S  E EK      IE   + E
Sbjct: 544  PVEAVDQGGPEKEETPPSVEPEEEEDTEDAGLDDWEAMASDEEREKEGNMIHIEVEENPE 603

Query: 713  IQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPA 772
             +E+E+++E + +S D+            +EE +SE  +G ++D K S     G  A   
Sbjct: 604  EEEEEEEEEEEEESEDE------------EEEGDSEGSDGDEEDCKLSDEKDSGK-AGDT 663

Query: 773  SPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSP 832
             P++ +       S D + TK   E    DK K +   ++K+    +       E LR+P
Sbjct: 664  KPSKDASSDSEYDSDD-DRTK---EERAYDKAKRR---IEKRRL--EHGKNVNTEKLRAP 723

Query: 833  ICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELK--ADAK 892
            I C++GHVDTGKTK+LD +R T+VQ+GEAGGITQQIGAT  P E I E+T+ +K      
Sbjct: 724  IICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDREN 783

Query: 893  LKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFI 952
            +++PG+LIIDTPGHESF+NLR+RGS LCD+AILVVDIMHGLEPQTIES+N+L+ +   FI
Sbjct: 784  VRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINILKSKKCPFI 843

Query: 953  IALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNK 1012
            +ALNK+DRLY WK   ++ +  T+K+Q K  ++EF  R   II +F +QGLN  L+Y+NK
Sbjct: 844  VALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENK 890

Query: 1013 EMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTT 1035
            +     S+VPTSA TG+G+  ++ LLV+  Q  ++K+L + +E++  V+EVK + G GTT
Sbjct: 904  DPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTT 890

BLAST of Cp4.1LG06g07750 vs. NCBI nr
Match: XP_023535151.1 (eukaryotic translation initiation factor 5B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1832 bits (4745), Expect = 0.0
Identity = 1034/1034 (100.00%), Postives = 1034/1034 (100.00%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDDVDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDGA 120
            GKKGNSKASQLKEDDDDDVDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDGA
Sbjct: 61   GKKGNSKASQLKEDDDDDVDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDGA 120

Query: 121  NDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDVI 180
            NDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDVI
Sbjct: 121  NDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDVI 180

Query: 181  DDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTIT 240
            DDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTIT
Sbjct: 181  DDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTIT 240

Query: 241  FSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKKK 300
            FSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKKK
Sbjct: 241  FSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKKK 300

Query: 301  SSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRTA 360
            SSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRTA
Sbjct: 301  SSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRTA 360

Query: 361  QEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTESA 420
            QEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTESA
Sbjct: 361  QEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTESA 420

Query: 421  AARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKHV 480
            AARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKHV
Sbjct: 421  AARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKHV 480

Query: 481  REMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRK 540
            REMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRK
Sbjct: 481  REMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRK 540

Query: 541  KQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQT 600
            KQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQT
Sbjct: 541  KQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQT 600

Query: 601  NGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDED 660
            NGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDED
Sbjct: 601  NGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDED 660

Query: 661  DDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQKS 720
            DDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQKS
Sbjct: 661  DDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQKS 720

Query: 721  LPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIMG 780
            LPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIMG
Sbjct: 721  LPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960
            LYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI
Sbjct: 901  LYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020
            VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIVVCGMQ 1034
            VLHEGDQIVVCGMQ
Sbjct: 1021 VLHEGDQIVVCGMQ 1034

BLAST of Cp4.1LG06g07750 vs. NCBI nr
Match: KAG6592168.1 (hypothetical protein SDJN03_14514, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1793 bits (4645), Expect = 0.0
Identity = 1019/1035 (98.45%), Postives = 1025/1035 (99.03%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDD-VDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKEDDDDD VD VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG
Sbjct: 61   GKKGNSKASQLKEDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180
            ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV
Sbjct: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180

Query: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTI 240
            IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLD EDEDRNDDMDDGEDVTTI
Sbjct: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDYEDEDRNDDMDDGEDVTTI 240

Query: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKK 300
            TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGV+EDDVNVISFSGKK
Sbjct: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVDEDDVNVISFSGKK 300

Query: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRT 360
            KSSKKKSSSTFTALSDENALGDEILNTASSNLDSD SKAKTEAVAE+SKNK+KKKKSGRT
Sbjct: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDLSKAKTEAVAETSKNKKKKKKSGRT 360

Query: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTES 420
            AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVE+QP    EVVAPPEKEVEEESTES
Sbjct: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVENQP----EVVAPPEKEVEEESTES 420

Query: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480
            AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH
Sbjct: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480

Query: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540
            VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR
Sbjct: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540

Query: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600
            KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ
Sbjct: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600

Query: 601  TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDE 660
            TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSD IEATVDNEIQEDE
Sbjct: 601  TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDAIEATVDNEIQEDE 660

Query: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQK 720
            DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPA+PAQK
Sbjct: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPAAPAQK 720

Query: 721  SLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780
            SLPSQPIKSQDIENTKKQHEVEVV+KGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM
Sbjct: 721  SLPSQPIKSQDIENTKKQHEVEVVEKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780

Query: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840
            GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL
Sbjct: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840

Query: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900
            IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD
Sbjct: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900

Query: 901  RLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960
            RLYGWKTIRNAPILKTMKQQSK VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS
Sbjct: 901  RLYGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960

Query: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020
            IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN
Sbjct: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020

Query: 1021 GVLHEGDQIVVCGMQ 1034
            GVLHEGDQIVVCGMQ
Sbjct: 1021 GVLHEGDQIVVCGMQ 1031

BLAST of Cp4.1LG06g07750 vs. NCBI nr
Match: KAG7025029.1 (SPAC56F8.03, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1793 bits (4645), Expect = 0.0
Identity = 1019/1035 (98.45%), Postives = 1025/1035 (99.03%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDD-VDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKEDDDDD VD VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG
Sbjct: 61   GKKGNSKASQLKEDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180
            ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV
Sbjct: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180

Query: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTI 240
            IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLD EDEDRNDDMDDGEDVTTI
Sbjct: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDYEDEDRNDDMDDGEDVTTI 240

Query: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKK 300
            TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGV+EDDVNVISFSGKK
Sbjct: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVDEDDVNVISFSGKK 300

Query: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRT 360
            KSSKKKSSSTFTALSDENALGDEILNTASSNLDSD SKAKTEAVAE+SKNK+KKKKSGRT
Sbjct: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDLSKAKTEAVAETSKNKKKKKKSGRT 360

Query: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTES 420
            AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVE+QP    EVVAPPEKEVEEESTES
Sbjct: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVENQP----EVVAPPEKEVEEESTES 420

Query: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480
            AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH
Sbjct: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480

Query: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540
            VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR
Sbjct: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540

Query: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600
            KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ
Sbjct: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600

Query: 601  TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDE 660
            TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSD IEATVDNEIQEDE
Sbjct: 601  TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDAIEATVDNEIQEDE 660

Query: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQK 720
            DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPA+PAQK
Sbjct: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPAAPAQK 720

Query: 721  SLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780
            SLPSQPIKSQDIENTKKQHEVEVV+KGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM
Sbjct: 721  SLPSQPIKSQDIENTKKQHEVEVVEKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780

Query: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840
            GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL
Sbjct: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840

Query: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900
            IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD
Sbjct: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900

Query: 901  RLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960
            RLYGWKTIRNAPILKTMKQQSK VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS
Sbjct: 901  RLYGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960

Query: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020
            IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN
Sbjct: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020

Query: 1021 GVLHEGDQIVVCGMQ 1034
            GVLHEGDQIVVCGMQ
Sbjct: 1021 GVLHEGDQIVVCGMQ 1031

BLAST of Cp4.1LG06g07750 vs. NCBI nr
Match: XP_022936059.1 (eukaryotic translation initiation factor 5B-like [Cucurbita moschata])

HSP 1 Score: 1792 bits (4642), Expect = 0.0
Identity = 1018/1035 (98.36%), Postives = 1025/1035 (99.03%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDD-VDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKEDDDDD VD VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG
Sbjct: 61   GKKGNSKASQLKEDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180
            ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV
Sbjct: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180

Query: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTI 240
            IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLD EDEDRNDDMDDGEDVTTI
Sbjct: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDYEDEDRNDDMDDGEDVTTI 240

Query: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKK 300
            TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGV+EDDVNVISFSGKK
Sbjct: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVDEDDVNVISFSGKK 300

Query: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRT 360
            KSSKKKSSSTFTALSDENALGDEILNTASSNLDSD SKAKTEAVAE+SKNK+KKKKSGRT
Sbjct: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDLSKAKTEAVAETSKNKKKKKKSGRT 360

Query: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTES 420
            AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVE+QP    EVVAPPEKEVEEESTES
Sbjct: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVENQP----EVVAPPEKEVEEESTES 420

Query: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480
            AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH
Sbjct: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480

Query: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540
            VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR
Sbjct: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540

Query: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600
            KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ
Sbjct: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600

Query: 601  TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDE 660
            TNGSAQTKVVEHVEERIQEKD+AETEILESEKIEAVESTLEEEKSD IEATVDNEIQEDE
Sbjct: 601  TNGSAQTKVVEHVEERIQEKDLAETEILESEKIEAVESTLEEEKSDAIEATVDNEIQEDE 660

Query: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQK 720
            DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPA+PAQK
Sbjct: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPAAPAQK 720

Query: 721  SLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780
            SLPSQPIKSQDIENTKKQHEVEVV+KGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM
Sbjct: 721  SLPSQPIKSQDIENTKKQHEVEVVEKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780

Query: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840
            GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL
Sbjct: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840

Query: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900
            IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD
Sbjct: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900

Query: 901  RLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960
            RLYGWKTIRNAPILKTMKQQSK VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS
Sbjct: 901  RLYGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960

Query: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020
            IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN
Sbjct: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020

Query: 1021 GVLHEGDQIVVCGMQ 1034
            GVLHEGDQIVVCGMQ
Sbjct: 1021 GVLHEGDQIVVCGMQ 1031

BLAST of Cp4.1LG06g07750 vs. NCBI nr
Match: XP_022974922.1 (eukaryotic translation initiation factor 5B-like [Cucurbita maxima])

HSP 1 Score: 1749 bits (4531), Expect = 0.0
Identity = 1002/1037 (96.62%), Postives = 1014/1037 (97.78%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKV+ITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVMITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDD--VDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD 120
            GKKGNSKASQLK+DDDDD  VD VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD
Sbjct: 61   GKKGNSKASQLKDDDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD 120

Query: 121  GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180
            GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED
Sbjct: 121  GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180

Query: 181  VIDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTT 240
            VIDDENEEIDDEPVISFTGKKK SSQGGKKAGNAFTGFSGLD EDEDRNDD DDGED TT
Sbjct: 181  VIDDENEEIDDEPVISFTGKKK-SSQGGKKAGNAFTGFSGLDYEDEDRNDDKDDGEDFTT 240

Query: 241  ITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGK 300
            +TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKP KLDIDGV+EDDVNVISFSGK
Sbjct: 241  MTFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPGKLDIDGVDEDDVNVISFSGK 300

Query: 301  KKSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGR 360
            KKSSKK SSSTFTALSDENALGDEILNTASSNLDS+ SKAKTEAVAE+SKNK+KKKKSGR
Sbjct: 301  KKSSKKNSSSTFTALSDENALGDEILNTASSNLDSNLSKAKTEAVAETSKNKKKKKKSGR 360

Query: 361  TAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTE 420
            TAQEEDDLDKILAELGEGPATSK ADPPLSSQEAKVE+QP    E+VAPPEKEVEEESTE
Sbjct: 361  TAQEEDDLDKILAELGEGPATSKSADPPLSSQEAKVENQP----ELVAPPEKEVEEESTE 420

Query: 421  SAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPK 480
            SAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPK
Sbjct: 421  SAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPK 480

Query: 481  HVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLL 540
            HVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLL
Sbjct: 481  HVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLL 540

Query: 541  RKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHH 600
            RKKQEGKLLTGKQKEEQRRLEAMRNQILSNAG LPLSTSDPSAPAKRPKYQTKKTKPAHH
Sbjct: 541  RKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGALPLSTSDPSAPAKRPKYQTKKTKPAHH 600

Query: 601  QTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIE-ATVDNEIQE 660
            QTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVES L EEKSDTIE ATVDNEIQE
Sbjct: 601  QTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESMLVEEKSDTIEEATVDNEIQE 660

Query: 661  DEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPA 720
            DEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKK+RKNSAPTSGGAVAKPA+PA
Sbjct: 661  DEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKNRKNSAPTSGGAVAKPAAPA 720

Query: 721  QKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICC 780
            QKSLPSQPIKSQDIENTKKQHEVEVVDKGK KDDAVK +TSIPDATPKQQEENLRSPICC
Sbjct: 721  QKSLPSQPIKSQDIENTKKQHEVEVVDKGKRKDDAVKNRTSIPDATPKQQEENLRSPICC 780

Query: 781  IMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPG 840
            IMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPG
Sbjct: 781  IMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPG 840

Query: 841  LLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNK 900
            LLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNK
Sbjct: 841  LLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNK 900

Query: 901  VDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGET 960
            VDRLYGWKT RNAPILKTMKQQSK VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGET
Sbjct: 901  VDRLYGWKTTRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGET 960

Query: 961  FSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVIL 1020
            FSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVIL
Sbjct: 961  FSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVIL 1020

Query: 1021 VNGVLHEGDQIVVCGMQ 1034
            VNGVLHEGDQIVVCGMQ
Sbjct: 1021 VNGVLHEGDQIVVCGMQ 1032

BLAST of Cp4.1LG06g07750 vs. ExPASy TrEMBL
Match: A0A6J1F778 (Eukaryotic translation initiation factor 5B OS=Cucurbita moschata OX=3662 GN=LOC111442774 PE=4 SV=1)

HSP 1 Score: 1792 bits (4642), Expect = 0.0
Identity = 1018/1035 (98.36%), Postives = 1025/1035 (99.03%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDD-VDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKEDDDDD VD VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG
Sbjct: 61   GKKGNSKASQLKEDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180
            ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV
Sbjct: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180

Query: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTI 240
            IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLD EDEDRNDDMDDGEDVTTI
Sbjct: 181  IDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDYEDEDRNDDMDDGEDVTTI 240

Query: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKK 300
            TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGV+EDDVNVISFSGKK
Sbjct: 241  TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVDEDDVNVISFSGKK 300

Query: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRT 360
            KSSKKKSSSTFTALSDENALGDEILNTASSNLDSD SKAKTEAVAE+SKNK+KKKKSGRT
Sbjct: 301  KSSKKKSSSTFTALSDENALGDEILNTASSNLDSDLSKAKTEAVAETSKNKKKKKKSGRT 360

Query: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTES 420
            AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVE+QP    EVVAPPEKEVEEESTES
Sbjct: 361  AQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVENQP----EVVAPPEKEVEEESTES 420

Query: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480
            AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH
Sbjct: 421  AAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKH 480

Query: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540
            VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR
Sbjct: 481  VREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLR 540

Query: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600
            KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ
Sbjct: 541  KKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQ 600

Query: 601  TNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEATVDNEIQEDE 660
            TNGSAQTKVVEHVEERIQEKD+AETEILESEKIEAVESTLEEEKSD IEATVDNEIQEDE
Sbjct: 601  TNGSAQTKVVEHVEERIQEKDLAETEILESEKIEAVESTLEEEKSDAIEATVDNEIQEDE 660

Query: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQK 720
            DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPA+PAQK
Sbjct: 661  DDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPAAPAQK 720

Query: 721  SLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780
            SLPSQPIKSQDIENTKKQHEVEVV+KGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM
Sbjct: 721  SLPSQPIKSQDIENTKKQHEVEVVEKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIM 780

Query: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840
            GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL
Sbjct: 781  GHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLL 840

Query: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900
            IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD
Sbjct: 841  IIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVD 900

Query: 901  RLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960
            RLYGWKTIRNAPILKTMKQQSK VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS
Sbjct: 901  RLYGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFS 960

Query: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020
            IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN
Sbjct: 961  IVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVN 1020

Query: 1021 GVLHEGDQIVVCGMQ 1034
            GVLHEGDQIVVCGMQ
Sbjct: 1021 GVLHEGDQIVVCGMQ 1031

BLAST of Cp4.1LG06g07750 vs. ExPASy TrEMBL
Match: A0A6J1IBK8 (Eukaryotic translation initiation factor 5B OS=Cucurbita maxima OX=3661 GN=LOC111473749 PE=4 SV=1)

HSP 1 Score: 1749 bits (4531), Expect = 0.0
Identity = 1002/1037 (96.62%), Postives = 1014/1037 (97.78%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKV+ITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVMITGKKK 60

Query: 61   GKKGNSKASQLKEDDDDD--VDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD 120
            GKKGNSKASQLK+DDDDD  VD VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD
Sbjct: 61   GKKGNSKASQLKDDDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD 120

Query: 121  GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180
            GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED
Sbjct: 121  GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180

Query: 181  VIDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTT 240
            VIDDENEEIDDEPVISFTGKKK SSQGGKKAGNAFTGFSGLD EDEDRNDD DDGED TT
Sbjct: 181  VIDDENEEIDDEPVISFTGKKK-SSQGGKKAGNAFTGFSGLDYEDEDRNDDKDDGEDFTT 240

Query: 241  ITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISFSGK 300
            +TFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKP KLDIDGV+EDDVNVISFSGK
Sbjct: 241  MTFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPGKLDIDGVDEDDVNVISFSGK 300

Query: 301  KKSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGR 360
            KKSSKK SSSTFTALSDENALGDEILNTASSNLDS+ SKAKTEAVAE+SKNK+KKKKSGR
Sbjct: 301  KKSSKKNSSSTFTALSDENALGDEILNTASSNLDSNLSKAKTEAVAETSKNKKKKKKSGR 360

Query: 361  TAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKEVEEESTE 420
            TAQEEDDLDKILAELGEGPATSK ADPPLSSQEAKVE+QP    E+VAPPEKEVEEESTE
Sbjct: 361  TAQEEDDLDKILAELGEGPATSKSADPPLSSQEAKVENQP----ELVAPPEKEVEEESTE 420

Query: 421  SAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPK 480
            SAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPK
Sbjct: 421  SAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPK 480

Query: 481  HVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLL 540
            HVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLL
Sbjct: 481  HVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLL 540

Query: 541  RKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKPAHH 600
            RKKQEGKLLTGKQKEEQRRLEAMRNQILSNAG LPLSTSDPSAPAKRPKYQTKKTKPAHH
Sbjct: 541  RKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGALPLSTSDPSAPAKRPKYQTKKTKPAHH 600

Query: 601  QTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDTIE-ATVDNEIQE 660
            QTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVES L EEKSDTIE ATVDNEIQE
Sbjct: 601  QTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESMLVEEKSDTIEEATVDNEIQE 660

Query: 661  DEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPA 720
            DEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKK+RKNSAPTSGGAVAKPA+PA
Sbjct: 661  DEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKNRKNSAPTSGGAVAKPAAPA 720

Query: 721  QKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICC 780
            QKSLPSQPIKSQDIENTKKQHEVEVVDKGK KDDAVK +TSIPDATPKQQEENLRSPICC
Sbjct: 721  QKSLPSQPIKSQDIENTKKQHEVEVVDKGKRKDDAVKNRTSIPDATPKQQEENLRSPICC 780

Query: 781  IMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPG 840
            IMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPG
Sbjct: 781  IMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPG 840

Query: 841  LLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNK 900
            LLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNK
Sbjct: 841  LLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNK 900

Query: 901  VDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGET 960
            VDRLYGWKT RNAPILKTMKQQSK VQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGET
Sbjct: 901  VDRLYGWKTTRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGET 960

Query: 961  FSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVIL 1020
            FSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVIL
Sbjct: 961  FSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVIL 1020

Query: 1021 VNGVLHEGDQIVVCGMQ 1034
            VNGVLHEGDQIVVCGMQ
Sbjct: 1021 VNGVLHEGDQIVVCGMQ 1032

BLAST of Cp4.1LG06g07750 vs. ExPASy TrEMBL
Match: A0A6J1E170 (Eukaryotic translation initiation factor 5B OS=Momordica charantia OX=3673 GN=LOC111025005 PE=4 SV=1)

HSP 1 Score: 1545 bits (4001), Expect = 0.0
Identity = 907/1054 (86.05%), Postives = 961/1054 (91.18%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTEL+EEAQ QE KVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELSEEAQVQEEKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDDD-DVDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKE++D+ D D VSEIVITGKKKGKSKKGGSSSAFTAS+FGLL+EEG DG
Sbjct: 61   GKKGNSKASQLKEEEDEEDGDGVSEIVITGKKKGKSKKGGSSSAFTASAFGLLDEEGNDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDEDV 180
            A DDDD+ ESV+T EKDDDE +DSVI FSGKKKSSKSSKK+GFSA  AF ALDDE DEDV
Sbjct: 121  AADDDDE-ESVVTGEKDDDEGDDSVINFSGKKKSSKSSKKTGFSA--AFSALDDENDEDV 180

Query: 181  IDDE---NEEIDDEPVISFTGKKKKSSQGGKKAGNAFT--GFSGLDDEDEDRNDDMDDGE 240
            ID+E   +E+IDDEPVI+FTGKKK SS+GGKKAGN FT  GFSGLD+EDED +   D+ E
Sbjct: 181  IDNEIRVDEDIDDEPVIAFTGKKK-SSKGGKKAGNTFTASGFSGLDNEDEDGDAKKDEDE 240

Query: 241  DVTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVIS 300
            D+ +I+FSGKKKKSSK SKKSGNLFSAA  DEENDGD SIS+P+KL++DGV+EDD  VI+
Sbjct: 241  DIASISFSGKKKKSSKASKKSGNLFSAAFPDEENDGDASISEPNKLNVDGVDEDDATVIA 300

Query: 301  FSGKKKSSKKKSSSTFTALSDENALGDE--------ILNTASSNLDSDSSKA-KTEAVAE 360
            FSGKKKSSKKK S   TALSDE+ LG+E        ILNTASSN+DSD SKA K E VAE
Sbjct: 301  FSGKKKSSKKKGSGMVTALSDESVLGNEVRDVVGSEILNTASSNIDSDLSKANKMEGVAE 360

Query: 361  SSKNKRKKKKSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVV 420
            +SKNK+KKKKSGRTAQEEDDLDKILAELGEGP TSKPADPPL SQE KVE+ P    E+V
Sbjct: 361  TSKNKKKKKKSGRTAQEEDDLDKILAELGEGPPTSKPADPPLPSQEDKVENPP----ELV 420

Query: 421  APP----EKEVEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIE 480
            APP    EKE EEESTESAAARKKKKKKEKEKEKKAAAAAAAAA   DEKIEEV TEIIE
Sbjct: 421  APPDASAEKEAEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAA--DEKIEEVNTEIIE 480

Query: 481  PKKGAAKGKVVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQ 540
            PKKGAAK KV EKKVPKHVREMQEAMARRKEEEERRKREEEE+LRKEEEERRRQEELERQ
Sbjct: 481  PKKGAAKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRQEELERQ 540

Query: 541  AEEAKRRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSA 600
            AEEAKRRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQIL+NAGGLPL+TSDPSA
Sbjct: 541  AEEAKRRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILANAGGLPLATSDPSA 600

Query: 601  PAKRPKYQTKKTKPAHHQTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLE- 660
            PAKRPKYQ+KKTKPAHHQTNGSAQTKVVEH+EE+ QEKDV ETE+LESEKIE VE  +  
Sbjct: 601  PAKRPKYQSKKTKPAHHQTNGSAQTKVVEHMEEKTQEKDVVETELLESEKIEDVEELMPV 660

Query: 661  EEKSDTIEATVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRK 720
            EEKSD +EAT DNE+QEDED+DEWDAKSWDDAVVDLSLKSSFADEELESE ENGMKKDRK
Sbjct: 661  EEKSDVVEATEDNEVQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDRK 720

Query: 721  NSAPTSGGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIP 780
            N AP +  A AKPA+PAQK+LPSQP+KSQDIEN KKQHE E+VDK K KDDAVKKK  IP
Sbjct: 721  NGAPAARDAGAKPAAPAQKNLPSQPLKSQDIENKKKQHEAEIVDKDKRKDDAVKKKAPIP 780

Query: 781  DATPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENI 840
            D TPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENI
Sbjct: 781  DVTPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENI 840

Query: 841  RERTRELKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIES 900
            RERTRELKADAKLKVPGLL+IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIES
Sbjct: 841  RERTRELKADAKLKVPGLLVIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIES 900

Query: 901  LNLLRMRNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKE 960
            LNLLRMRNTEFI+ALNKVDRLYGWKTIRNAPILKTMKQQSK VQNEFNMRLIQIITQFKE
Sbjct: 901  LNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKE 960

Query: 961  QGLNTELYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTV 1020
            QGLNTELYYKNKEMGETFSIVPTSAVTGEGIPD+LLLLVQWAQKTMT+KLTYSDEVQCTV
Sbjct: 961  QGLNTELYYKNKEMGETFSIVPTSAVTGEGIPDLLLLLVQWAQKTMTEKLTYSDEVQCTV 1020

Query: 1021 LEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1034
            LEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ
Sbjct: 1021 LEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1044

BLAST of Cp4.1LG06g07750 vs. ExPASy TrEMBL
Match: A0A5D3BXH6 (Eukaryotic translation initiation factor 5B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00600 PE=4 SV=1)

HSP 1 Score: 1539 bits (3984), Expect = 0.0
Identity = 914/1048 (87.21%), Postives = 957/1048 (91.32%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDD APAAA GGGKSKKKTFAVDDDEYSIGTEL+EEAQ QE KVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDD-DDVDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKEDDD DD D VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEG DG
Sbjct: 61   GKKGNSKASQLKEDDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDS-VIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180
            A   DDD+ESVLTAEKDDD+EE+   IKFSGKKKSSKSSKKSGFSAVSAF ALDDE DED
Sbjct: 121  A---DDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED 180

Query: 181  VIDDE---NEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGED 240
             ID+E   +E+ID EPV+ FTGKKK SS+GGKKA +AF+GFSGLD ED+DR DD  D ED
Sbjct: 181  AIDNEIRVDEDID-EPVVEFTGKKK-SSKGGKKALSAFSGFSGLDYEDDDR-DDQKDEED 240

Query: 241  VTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISF 300
            V +I+FSGKKKKS+K SKKSGNLFSAALADEENDGD S+S+ +KLD DGV+EDDVNVI+F
Sbjct: 241  VASISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAF 300

Query: 301  SGKKKSSKKKSSSTFTALSDENALGDE--------ILNTASSNLDSDSSKA-KTEAVAES 360
            SGKKKSSKKK++STFTALSDENA G+E        I NT SSNLDSD S A KTEA+AE+
Sbjct: 301  SGKKKSSKKKNNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAET 360

Query: 361  SKNKRKKKKSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVA 420
            SKNK+KKKKSGRTAQEEDDLDKILAELGEGPA SKPADPPL SQEAKVE+ P    E+VA
Sbjct: 361  SKNKKKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPP----ELVA 420

Query: 421  PPEKEVEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGA 480
            PPEKE EE+STESAAARKKKKKKEKEKEKKAAAAA AAAEGNDEKIEEVKTEIIEPKKGA
Sbjct: 421  PPEKEAEEDSTESAAARKKKKKKEKEKEKKAAAAA-AAAEGNDEKIEEVKTEIIEPKKGA 480

Query: 481  AKGKVVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAK 540
            AK KV EKKVPKHVREMQEAMARRKEEEERRKREEEE+L+KEEEER R EELERQAEEAK
Sbjct: 481  AKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAK 540

Query: 541  RRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRP 600
            RRKKEREKEKLLRKK EGKLLTGKQKEEQRRLEAMR QILSN GGLPLSTSDPSAPAKRP
Sbjct: 541  RRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRP 600

Query: 601  KYQTKKTKPAHHQTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDT 660
            KYQTKKTKP+HHQTNG+AQTK VEH+EE+IQEKDVAETE+LESEKIEAVE    EEKS  
Sbjct: 601  KYQTKKTKPSHHQTNGNAQTKAVEHIEEKIQEKDVAETEVLESEKIEAVELMHVEEKSGI 660

Query: 661  IEATVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTS 720
            +EAT DNEIQEDED+DEWDAKSWDDAVVDLSLKSSFADEELESE EN MKKDRKN     
Sbjct: 661  LEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKN----- 720

Query: 721  GGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQ 780
             GA AK A+PAQK LPSQ IKSQDIEN KKQ EVEV DKGK K+DAV+KK SIPDATP Q
Sbjct: 721  -GAGAKLAAPAQKGLPSQSIKSQDIENKKKQDEVEVPDKGKRKEDAVRKKASIPDATPVQ 780

Query: 781  QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE 840
            QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE
Sbjct: 781  QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE 840

Query: 841  LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM 900
            LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM
Sbjct: 841  LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM 900

Query: 901  RNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTE 960
            RNTEFI+ALNKVDRLYGWKTIRNAPILKTMKQQ+K VQNEFNMRLIQIITQFKEQGLNTE
Sbjct: 901  RNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTE 960

Query: 961  LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVV 1020
            LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVV
Sbjct: 961  LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVV 1020

Query: 1021 EGHGTTIDVILVNGVLHEGDQIVVCGMQ 1034
            EGHGTTIDVILVNGVLHEGDQIVVCGMQ
Sbjct: 1021 EGHGTTIDVILVNGVLHEGDQIVVCGMQ 1031

BLAST of Cp4.1LG06g07750 vs. ExPASy TrEMBL
Match: A0A5A7U6Q9 (Eukaryotic translation initiation factor 5B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2606G00100 PE=4 SV=1)

HSP 1 Score: 1539 bits (3984), Expect = 0.0
Identity = 914/1048 (87.21%), Postives = 957/1048 (91.32%), Query Frame = 0

Query: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60
            MGRKKPTARDDD APAAA GGGKSKKKTFAVDDDEYSIGTEL+EEAQ QE KVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQLKEDDD-DDVDDVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKDG 120
            GKKGNSKASQLKEDDD DD D VSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEG DG
Sbjct: 61   GKKGNSKASQLKEDDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMDG 120

Query: 121  ANDDDDDDESVLTAEKDDDEEEDS-VIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180
            A   DDD+ESVLTAEKDDD+EE+   IKFSGKKKSSKSSKKSGFSAVSAF ALDDE DED
Sbjct: 121  A---DDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED 180

Query: 181  VIDDE---NEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGED 240
             ID+E   +E+ID EPV+ FTGKKK SS+GGKKA +AF+GFSGLD ED+DR DD  D ED
Sbjct: 181  AIDNEIRVDEDID-EPVVEFTGKKK-SSKGGKKALSAFSGFSGLDYEDDDR-DDQKDEED 240

Query: 241  VTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVISF 300
            V +I+FSGKKKKS+K SKKSGNLFSAALADEENDGD S+S+ +KLD DGV+EDDVNVI+F
Sbjct: 241  VASISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAF 300

Query: 301  SGKKKSSKKKSSSTFTALSDENALGDE--------ILNTASSNLDSDSSKA-KTEAVAES 360
            SGKKKSSKKK++STFTALSDENA G+E        I NT SSNLDSD S A KTEA+AE+
Sbjct: 301  SGKKKSSKKKNNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAET 360

Query: 361  SKNKRKKKKSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVA 420
            SKNK+KKKKSGRTAQEEDDLDKILAELGEGPA SKPADPPL SQEAKVE+ P    E+VA
Sbjct: 361  SKNKKKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPP----ELVA 420

Query: 421  PPEKEVEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGA 480
            PPEKE EE+STESAAARKKKKKKEKEKEKKAAAAA AAAEGNDEKIEEVKTEIIEPKKGA
Sbjct: 421  PPEKEAEEDSTESAAARKKKKKKEKEKEKKAAAAA-AAAEGNDEKIEEVKTEIIEPKKGA 480

Query: 481  AKGKVVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAK 540
            AK KV EKKVPKHVREMQEAMARRKEEEERRKREEEE+L+KEEEER R EELERQAEEAK
Sbjct: 481  AKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAK 540

Query: 541  RRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRP 600
            RRKKEREKEKLLRKK EGKLLTGKQKEEQRRLEAMR QILSN GGLPLSTSDPSAPAKRP
Sbjct: 541  RRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRP 600

Query: 601  KYQTKKTKPAHHQTNGSAQTKVVEHVEERIQEKDVAETEILESEKIEAVESTLEEEKSDT 660
            KYQTKKTKP+HHQTNG+AQTK VEH+EE+IQEKDVAETE+LESEKIEAVE    EEKS  
Sbjct: 601  KYQTKKTKPSHHQTNGNAQTKAVEHIEEKIQEKDVAETEVLESEKIEAVELMHVEEKSGI 660

Query: 661  IEATVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTS 720
            +EAT DNEIQEDED+DEWDAKSWDDAVVDLSLKSSFADEELESE EN MKKDRKN     
Sbjct: 661  LEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKN----- 720

Query: 721  GGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQ 780
             GA AK A+PAQK LPSQ IKSQDIEN KKQ EVEV DKGK K+DAV+KK SIPDATP Q
Sbjct: 721  -GAGAKLAAPAQKGLPSQSIKSQDIENKKKQDEVEVPDKGKRKEDAVRKKASIPDATPVQ 780

Query: 781  QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE 840
            QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE
Sbjct: 781  QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE 840

Query: 841  LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM 900
            LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM
Sbjct: 841  LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM 900

Query: 901  RNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTE 960
            RNTEFI+ALNKVDRLYGWKTIRNAPILKTMKQQ+K VQNEFNMRLIQIITQFKEQGLNTE
Sbjct: 901  RNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTE 960

Query: 961  LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVV 1020
            LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVV
Sbjct: 961  LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVV 1020

Query: 1021 EGHGTTIDVILVNGVLHEGDQIVVCGMQ 1034
            EGHGTTIDVILVNGVLHEGDQIVVCGMQ
Sbjct: 1021 EGHGTTIDVILVNGVLHEGDQIVVCGMQ 1031

BLAST of Cp4.1LG06g07750 vs. TAIR 10
Match: AT1G76810.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 864.4 bits (2232), Expect = 9.4e-251
Identity = 589/1059 (55.62%), Postives = 721/1059 (68.08%), Query Frame = 0

Query: 1    MGRKKPTARDDDG---APAAA-QGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVK-VVI 60
            MGRKKP+AR  D     PA++  G  KSKKK   +DDDEYSIGTEL+EE++ +E K VVI
Sbjct: 1    MGRKKPSARGGDAEQQPPASSLVGATKSKKKGAQIDDDEYSIGTELSEESKVEEEKVVVI 60

Query: 61   TGKKKGKKGNSKASQLKEDDDDDV----------DDVSEIVITGKKKGKSKKGGSSSAFT 120
            TGKKKGKKGN K +Q  +DDDDD           DDV EI   GKKK K KKGG S    
Sbjct: 61   TGKKKGKKGNKKGTQ--QDDDDDFSDKVSAAGVKDDVPEIAFVGKKKSKGKKGGGS---- 120

Query: 121  ASSFGLLEEEGKDGANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAV 180
              SF LL+                      D+DE+E                        
Sbjct: 121  -VSFALLD----------------------DEDEKE------------------------ 180

Query: 181  SAFGALDDEKDEDVIDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAF--TGFSGLDDED 240
                  D+E D D         DDEPVISFTG KK +S+ GKK GN+F  + F  L  +D
Sbjct: 181  ------DNESDGDK--------DDEPVISFTG-KKHASKKGKKGGNSFAASAFDALGSDD 240

Query: 241  EDRNDDMDDGEDVTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDID 300
            +D  +  +D E+ + ITFSGKKKKSSK SKK+ N F+A L DEE   D S S+ D+   +
Sbjct: 241  DDTEEVHEDEEEESPITFSGKKKKSSKSSKKNTNSFTADLLDEEEGTDASNSRDDE---N 300

Query: 301  GVEEDDVNVISFSGKKKSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAV 360
             +E+++   ++FSGKKKSSKKK  S   ++ D+ ++ DE          + +S  K   V
Sbjct: 301  TIEDEESPEVTFSGKKKSSKKKGGSVLASVGDD-SVADE----------TKTSDTKNVEV 360

Query: 361  AESSKNKRKKK--KSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQ 420
             E+ K+K+KKK  KSGRT QEE+DLDK+LA LGE PA  +PA      ++A       +Q
Sbjct: 361  VETGKSKKKKKNNKSGRTVQEEEDLDKLLAALGETPAAERPASSTPVEEKA-------AQ 420

Query: 421  PEVVAPPEKEVEEE-STESAAARKKKKKKEKEKEKKAAAAAAA--AAEGNDEKIEEVKTE 480
            PE VAP E   E+E   E+AAA+KKKKKKEKEKEKKAAAAAAA  + E  +EK EE  TE
Sbjct: 421  PEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKKAAAAAAATSSVEVKEEKQEESVTE 480

Query: 481  IIEPKKGAAKGKVVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEEL 540
             ++PKK  AKGK  EKK+PKHVREMQEA+ARR+E EER+K+EEEEKLRKEEEERRRQEEL
Sbjct: 481  PLQPKKKDAKGKAAEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEEERRRQEEL 540

Query: 541  ERQAEEAKRRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSD 600
            E QAEEAKR++KE+EKEKLLRKK EGKLLT KQK E ++ EA +NQ+L+  GGLP++ +D
Sbjct: 541  EAQAEEAKRKRKEKEKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAGGGLPVADND 600

Query: 601  PSA-PAKRPKYQTKKTKPAHHQTNGSAQTK-VVEHVEERIQEKD-VAETEILESEKIEAV 660
              A  +KRP Y  KK        + S Q +  VE  E +  E+D + E  + ++ K++ +
Sbjct: 601  GDATSSKRPIYANKKKSSRQKGIDTSVQGEDEVEPKENQADEQDTLGEVGLTDTGKVDLI 660

Query: 661  ESTLEEEKSDTIEATVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGM 720
            E    +E S   +   +N ++ED+++DEWDAKSW    VDL+LK  F DE  E E++  +
Sbjct: 661  ELVNTDENSGPADVAQENGVEEDDEEDEWDAKSW--GTVDLNLKGDFDDE--EEEAQPVV 720

Query: 721  KKDRKNSAPTSGGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKK 780
            KK+ K++   +  +  +   P  K   +       ++ T +  +     +     DA KK
Sbjct: 721  KKELKDAISKAHDSEPEAEKPTAKPAGTGKPLIAAVKATPEVEDATRTKRATRAKDASKK 780

Query: 781  KTSIPDATPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYF 840
               +  +   + EENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYF
Sbjct: 781  GKGLAPSESIEGEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYF 840

Query: 841  PAENIRERTRELKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEP 900
            PAENIRERT+ELKADAKLKVPGLL+IDTPGHESFTNLRSRGS LCDLAILVVDIMHGLEP
Sbjct: 841  PAENIRERTKELKADAKLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDIMHGLEP 900

Query: 901  QTIESLNLLRMRNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQII 960
            QTIESLNLLRMRNTEFI+ALNKVDRLYGWKT +NAPI+K MKQQ+K V NEFN+RL  II
Sbjct: 901  QTIESLNLLRMRNTEFIVALNKVDRLYGWKTCKNAPIVKAMKQQNKDVINEFNLRLKNII 960

Query: 961  TQFKEQGLNTELYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDE 1020
             +F+EQGLNTELYYKNK+MG+TFSIVPTSA++GEG+PD+LL LVQWAQKTM +KLTY DE
Sbjct: 961  NEFQEQGLNTELYYKNKDMGDTFSIVPTSAISGEGVPDLLLWLVQWAQKTMVEKLTYVDE 966

Query: 1021 VQCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1035
            VQCTVLEVKV+EGHGTTIDV+LVNG LHEGDQIVVCG+Q
Sbjct: 1021 VQCTVLEVKVIEGHGTTIDVVLVNGELHEGDQIVVCGLQ 966

BLAST of Cp4.1LG06g07750 vs. TAIR 10
Match: AT1G76720.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 729.9 bits (1883), Expect = 2.8e-210
Identity = 549/1047 (52.44%), Postives = 670/1047 (63.99%), Query Frame = 0

Query: 1    MGRKKPTAR---DDDGAPAAAQ-GGGKSKKKTFAVD-DDEYSIGTELTEEAQDQEVKVVI 60
            MGRKKP++    DD+  PAA+   G +SKKK    D +D+YSI TE        E KVVI
Sbjct: 1    MGRKKPSSSRGGDDEQPPAASSLVGAESKKKAVQSDEEDKYSINTE--------EEKVVI 60

Query: 61   TGKKKGKKGNSKASQLKEDDDDDVDDVSEIVITG-KKKGKSKKGGSSSAFTASSFGLLEE 120
            TGK   KK N K +Q  +DDDD  + V E    G KKK K K  G S +F   S      
Sbjct: 61   TGK---KKSNKKVTQKHDDDDDFTEAVPENGFVGKKKKSKGKNRGGSVSFALLS------ 120

Query: 121  EGKDGANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDE 180
                   ++ DD+ES      + D++++ VI F+GKK +S   KK GF AVSAF AL  +
Sbjct: 121  -----GKEETDDNES------NGDKDDEPVISFTGKKNASNKGKK-GF-AVSAFDALGGD 180

Query: 181  KDEDVIDDENEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDDEDEDRNDDMDDGE 240
            KD++ +D + E++     I+F       S+G                        M D E
Sbjct: 181  KDDEEVDGDEEQVSP---ITF-------SEG-----------------------TMGD-E 240

Query: 241  DVTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVEEDDVNVIS 300
            +   ITFSGKKK         G++  A+L      GDDS++                   
Sbjct: 241  ESLEITFSGKKK---------GSIVLASL------GDDSVA------------------- 300

Query: 301  FSGKKKSSKKKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVAESSKNKRKKK 360
                 ++S+ K+  T                             K+  V E+ K K+KKK
Sbjct: 301  ----DETSQAKTPDT-----------------------------KSVEVIETGKIKKKKK 360

Query: 361  ---KSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVESQPKSQPEVVAPPEKE 420
               K  RT +EEDDLDK+LAELGE PA  +PA    + +  KV++QP     V    EKE
Sbjct: 361  NKNKVARTLEEEDDLDKLLAELGETPAAERPASS--TPEVEKVQAQPGPVAPVENAGEKE 420

Query: 421  VEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGKV 480
             E+E+ E+AAA+KKKKKKEK+KEKKAAAAA ++ E  +EK EE  TE ++PKK  AKGK 
Sbjct: 421  GEKETVETAAAKKKKKKKEKDKEKKAAAAATSSVEAKEEKQEESVTEPLQPKKKDAKGKA 480

Query: 481  VEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKKE 540
             EKK+PKHVREMQEA+ARR+E EER+K+EEEEKLRKEEEERRRQEELE QAEEAKR++KE
Sbjct: 481  AEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKE 540

Query: 541  REKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTS-DPSAPAKRPKYQT 600
            +EKEKLLRKK EGKLLT KQK E ++ EA +NQ+L+   GLP++     +  +KRP Y  
Sbjct: 541  KEKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAGRGLPVADDVGDATSSKRPIYAN 600

Query: 601  KKTKPAHHQTNGSAQTKVVEHVE-ERIQEKDVAETEILESEKIEAVESTLEEEKSDTIEA 660
            KK KP+  + N ++  +V + VE +      + E    ++EK++ +ES    EKS   + 
Sbjct: 601  KK-KPSRQKGNDTS-VQVEDEVEPQENHAATLGEVGSEDTEKVDLLESANTGEKSGPADV 660

Query: 661  TVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGA 720
              +N +   E+DDEWDAKSWD+  VDL +      ++ E E++  +KK+ K  A  S   
Sbjct: 661  AQENGV---EEDDEWDAKSWDN--VDLKI------DDKEEEAQPVVKKELK--AHDSDHE 720

Query: 721  VAKP-ASPAQKS-LPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAVKKKTSIPDATPKQQ 780
              KP A PA  S L +  +K+            EV D       A K K   P    K+ 
Sbjct: 721  TEKPTAKPAGMSKLTTGAVKAIS----------EVEDAATQTKRAKKGKCLAPSEFIKEG 780

Query: 781  EENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTREL 840
             ENLRS ICCIMGHVD+GKTKLLDCIRGTNVQEGEAGGITQQIGATYFPA+NIRERTREL
Sbjct: 781  GENLRSIICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAKNIRERTREL 840

Query: 841  KADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMR 900
            KADAKLKVPGLL+IDTPGHESFTNLRSRGS LCDLAILVVDI HGLEPQTIESLNLLRMR
Sbjct: 841  KADAKLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDIKHGLEPQTIESLNLLRMR 889

Query: 901  NTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTEL 960
            NTEFIIALNKVDRLYGWKT +NAPI+K MKQQ+K V NEFN+RL  IITQFKEQGLNTE+
Sbjct: 901  NTEFIIALNKVDRLYGWKTCKNAPIVKAMKQQNKDVTNEFNIRLTDIITQFKEQGLNTEI 889

Query: 961  YYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVE 1020
            YYKNKEMGETFSIVPTSA +GEG+PD+LL LVQWAQKTM +KLTY DEVQCTVLEVKV+E
Sbjct: 961  YYKNKEMGETFSIVPTSAKSGEGVPDLLLWLVQWAQKTMVEKLTYVDEVQCTVLEVKVIE 889

Query: 1021 GHGTTIDVILVNGVLHEGDQIVVCGMQ 1035
            GHGTTIDV+LVNG LHEGDQIVVCG+Q
Sbjct: 1021 GHGTTIDVVLVNGELHEGDQIVVCGLQ 889

BLAST of Cp4.1LG06g07750 vs. TAIR 10
Match: AT1G76820.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 701.4 bits (1809), Expect = 1.1e-201
Identity = 490/897 (54.63%), Postives = 598/897 (66.67%), Query Frame = 0

Query: 150  KKKSSKSSKKSGFSAVSAFGALDDEKDEDVIDDENEEIDDEPVIS-----FTGKKKKSSQ 209
            K+ SS+        A S  G+  D+K   + DDE     +EP +       TGKKK    
Sbjct: 4    KEPSSRGGDDEQPLASSLVGSKSDKKSVQIDDDEYSICLEEPKVEENKVVITGKKK---- 63

Query: 210  GGKKAGNAFTGFSGLDDEDEDRNDDMDDGEDVTTITFSGKKKKSSKGSKKSGNLFSAALA 269
             GKK      G     D+D+D  D     E+V          K SKG K  G++  A LA
Sbjct: 64   -GKKRNK--KGTQQQQDDDDDFADKFAVEEEVVPDNAFVGNNKKSKGKKDGGSVSFALLA 123

Query: 270  DEENDGDDSISKPDKLDIDGVEEDDVNVISFSGKKKSSKKKSSSTFTALSDENALGDEIL 329
            DE+N                 EE + N I+FSG+KKSSKKKSSS   ++ DE        
Sbjct: 124  DEDNR----------------EEAEDNDITFSGRKKSSKKKSSSVLASIGDEE------- 183

Query: 330  NTASSNLDSDSSKAKTEAVAESSKNKRKKKKSGRTAQEEDDLDKILAELGEGPATSKPAD 389
              +S +  SDS   +    A S K+K KK KSGRT QE+DDLDK+LAELGE PA  KPA 
Sbjct: 184  --SSQSKTSDSKSVELVESARSKKHK-KKNKSGRTLQEDDDLDKLLAELGETPAAGKPAS 243

Query: 390  PPLSSQEAKVESQPKSQPEVVAPPEKEVEEES---TESAAARKKKKKKEKEKEKKAAAAA 449
                      E + ++QPE VAP E   E+E          RK+K+++ K+++KKAAAAA
Sbjct: 244  E---------EEKDQAQPEPVAPVENTGEKEKRRLLRLLQPRKRKRRRRKKRKKKAAAAA 303

Query: 450  AA--AAEGNDEKIEEVKTEIIEPKKGAAKGKVVEKKVPKHVREMQEAMARRKEEEERRKR 509
            AA  + E  +EK EE  TE ++P+K  AKGK  EKK+PKHVRE+QEA+ARR+E +ER+K+
Sbjct: 304  AATSSVEAKEEKQEESVTEPLQPRKKDAKGKAAEKKIPKHVREIQEALARRQEAKERKKK 363

Query: 510  EEEEKLRKEEEERRRQEELERQAEEAKRRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLE 569
            EEEEKLRKEEEERRRQEEL+ QAEEAKR++KE+EKEKLLRKK EGKLLT KQK E ++ E
Sbjct: 364  EEEEKLRKEEEERRRQEELDAQAEEAKRKRKEKEKEKLLRKKLEGKLLTAKQKSEAQKRE 423

Query: 570  AMRNQILSNAGGLPLSTSDPSA-PAKRPKYQTKKTKPAHHQTNGSAQTKVVEHVEERIQE 629
            A +NQ+L+  GGLP++  D  A  +KRP Y  KK        + S Q   VE   E  + 
Sbjct: 424  AFKNQLLAAGGGLPVADDDDDATSSKRPIYANKKKSSRQKGNDTSVQ---VEGEVEPKEN 483

Query: 630  KDVAETEILESEKIEAVES-TLEEEKSDTIEATVDNEIQEDEDDDEWDAKSWDDAVVDLS 689
              + E    ++EK++ +ES  L E+     +   +N +   E+DDEWDAKSWD+  VDL 
Sbjct: 484  HALGEVGSADTEKVDLLESANLGEKSGGPADVAQENGV---EEDDEWDAKSWDN--VDLK 543

Query: 690  LKSSFADEELESESENGMKKDRKNSAPTSGGAVAKPASPAQKSLPSQPIKSQDIENTKKQ 749
            ++  F D+  E E+++ +KK+ K  A  S     KP         ++P     +E    +
Sbjct: 544  IRGDFDDK--EEEAQHVVKKEFK--AHYSDHETEKPT--------AKPAGMSKLETAAVK 603

Query: 750  HEVEVVDKGKGKDDAVKKKTSIPDATPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTN 809
               EV D       A K K   P+   ++  E LRS ICCIMGHVD+GKTKLLDCIRGTN
Sbjct: 604  AISEVEDAATQTKRAKKGKCLAPNEFIEEGGEKLRSIICCIMGHVDSGKTKLLDCIRGTN 663

Query: 810  VQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLIIDTPGHESFTNLRSRGS 869
            VQEGEAGGITQQIGATYFPA+NIRERTRELKADAKLKVPGLL+IDTPGHESFTNLRSRGS
Sbjct: 664  VQEGEAGGITQQIGATYFPAKNIRERTRELKADAKLKVPGLLVIDTPGHESFTNLRSRGS 723

Query: 870  GLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIIALNKVDRLYGWKTIRNAPILKTMK 929
             LCDLAILVVDI HGL+PQTIESLNLLRMRNTEFIIALNKVDRLYGWKT +NAPI+K MK
Sbjct: 724  SLCDLAILVVDITHGLQPQTIESLNLLRMRNTEFIIALNKVDRLYGWKTCKNAPIVKAMK 783

Query: 930  QQSKYVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSIVPTSAVTGEGIPDMLLL 989
            QQ+K V NEFN+RL +II +F+EQGLNTELYYKNK+MGETFSIVPTSA++GEG+PD+LL 
Sbjct: 784  QQNKDVINEFNLRLKKIINEFQEQGLNTELYYKNKDMGETFSIVPTSAISGEGVPDLLLW 838

Query: 990  LVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1035
            LVQWAQKTM +KLTY DEVQCTVLEVKV+EGHGTTIDV+LVNG LHEGDQIVVCG+Q
Sbjct: 844  LVQWAQKTMVEKLTYVDEVQCTVLEVKVIEGHGTTIDVVLVNGELHEGDQIVVCGLQ 838

BLAST of Cp4.1LG06g07750 vs. TAIR 10
Match: AT1G21160.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 1.7e-151
Identity = 384/761 (50.46%), Postives = 498/761 (65.44%), Query Frame = 0

Query: 286  EEDDVNVISFSGKKKSSK-KKSSSTFTALSDENALGDEILNTASSNLDSDSSKAKTEAVA 345
            E+     I FS +KK  K KK  S   AL D     +E    A    D + S    E V 
Sbjct: 45   EKPKAEEIDFSARKKKLKSKKGGSVSFALLDVEDDKEE----AEDGDDKNPSIRSDEVVG 104

Query: 346  ESSKNKRKKK-KSGR--TAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVE-SQPKS 405
            E+S +K+KKK KSG+    +E+DD DKILA+ G     S       +  EA++  +QP+ 
Sbjct: 105  ETSMSKKKKKDKSGKHGVCEEDDDADKILAQHGITTTVS-------TGPEAEIALAQPEQ 164

Query: 406  QPEVVAPPEKEVEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEII 465
                    +K+ EE++ ESA A+KKKKKK+K+K K +A+ A ++ E  +++         
Sbjct: 165  VASADGAVDKDGEEKTVESATAKKKKKKKDKDK-KASASLAISSVEAKEDR--------- 224

Query: 466  EPKKGAAKGKVVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELER 525
               K   K KV EKKVPKHVRE QE +AR KE E+ +K+EEEE+LRKEEEERR +EE ER
Sbjct: 225  -QGKKDVKIKVAEKKVPKHVREKQETLARWKEAEDGKKKEEEERLRKEEEERRIEEERER 284

Query: 526  QAEEAKRRKKEREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPS 585
            +AEE ++++K R+ E    KKQEG +LT KQK +  + EA R ++L++AG L ++  +  
Sbjct: 285  EAEEIRQKRKIRKME----KKQEGLILTAKQKRDAAKNEAFRKRVLTDAGSLLVADKNGD 344

Query: 586  APAKRPKYQTKK-------TKPAHHQTNGSAQTKVVEHVEERIQEKDVAETEILESEKIE 645
            + +KRP Y  K          PA  Q  G  +TK     +E     D+     +E  ++ 
Sbjct: 345  S-SKRPIYGNKNKLACKKANDPASVQAKGDGETKENHAADEPCTLPDLVS---VEDRRVG 404

Query: 646  AVESTLEEEKSDTIEATVDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESEN 705
             +ES   EE  ++++ + +N     +++D WDAK+      + ++K    DE  E + + 
Sbjct: 405  IIESVDTEETHESVDVSQEN----GDEEDVWDAKT------NFTIKGDSDDE--EEKPQP 464

Query: 706  GMKKDRKNSAPTSGGAVAKPASPAQKSLPSQPIKSQDIENTKKQHEVEVVDKGKGKDDAV 765
              KK  K++A  +  +V     P  K     P  S   +   K+   +V D  + KD + 
Sbjct: 465  VFKKGLKDTASKAHDSVPGADRPTVK-----PGGSGKPKTAAKKAMPKVDDTTRPKDTSK 524

Query: 766  KKKTSIPDATPKQQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGAT 825
            K +  + +   K+ EENLRSPICCIMGHVD+GKTKLLDCIRGTNVQEGEAGGITQQIGAT
Sbjct: 525  KDEGLVLNELAKEVEENLRSPICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGAT 584

Query: 826  YFPAENIRERTRELKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGL 885
            +FPAENIRERT+EL+A+AKLKVPG+L+IDTPGHESFTNLRSRGS LCDLAILVVDIM GL
Sbjct: 585  FFPAENIRERTKELQANAKLKVPGILVIDTPGHESFTNLRSRGSNLCDLAILVVDIMRGL 644

Query: 886  EPQTIESLNLLRMRNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQ 945
            EPQTIESLNLLR RN +FIIALNKVDRLYGW+  +NAPI KTM QQ+  V  EF MRL +
Sbjct: 645  EPQTIESLNLLRRRNVKFIIALNKVDRLYGWEKSKNAPIRKTMMQQTGDVVKEFKMRLNR 704

Query: 946  IITQFKEQGLNTELYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYS 1005
            +  QF+EQGLN+ LYYKN+EMGET SI+P SA++GEGIPD+LL LVQWAQKTM +KLTY 
Sbjct: 705  VQNQFQEQGLNSMLYYKNREMGETISILPASAISGEGIPDLLLFLVQWAQKTMVEKLTYV 758

Query: 1006 DEVQCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1035
            D+VQCTVLEVKV+EGHG T+DV+LVNGVL EGDQIVVCG Q
Sbjct: 765  DKVQCTVLEVKVIEGHGITVDVVLVNGVLREGDQIVVCGSQ 758

BLAST of Cp4.1LG06g07750 vs. TAIR 10
Match: AT2G27700.1 (eukaryotic translation initiation factor 2 family protein / eIF-2 family protein )

HSP 1 Score: 415.6 bits (1067), Expect = 1.2e-115
Identity = 209/269 (77.70%), Postives = 235/269 (87.36%), Query Frame = 0

Query: 767  QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE 826
            +E+ +RSPICC+MGH+DTGKTKLLD I G NVQEGEAGG TQ++GATY  A NI E+T E
Sbjct: 30   EEDKVRSPICCMMGHIDTGKTKLLDYITG-NVQEGEAGGTTQKMGATYLSARNILEKTME 89

Query: 827  LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM 886
            LK+D KLKVP  L IDTPG+E +TNLRSRG GLCD AILVVDIMHGLEPQTIE LNLLRM
Sbjct: 90   LKSDTKLKVPRPLFIDTPGYEFYTNLRSRGLGLCDFAILVVDIMHGLEPQTIECLNLLRM 149

Query: 887  RNTEFIIALNKVDRLYGWKTIRNAPILKTMKQQSKYVQNEFNMRLIQIITQFKEQGLNTE 946
            +NTEFIIALNKVDRLYGW+   NAPIL+ MKQQ+K V NEFNMRL +II QFKEQGLN+E
Sbjct: 150  KNTEFIIALNKVDRLYGWRICENAPILEAMKQQTKDVINEFNMRLNEIINQFKEQGLNSE 209

Query: 947  LYYKNKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKL-TYSDEVQCTVLEVKV 1006
            LYYKNKEMGETFSIVPT A++GEGIPD+LLLLVQ  QKTM +KL TY D+VQCTVLEVKV
Sbjct: 210  LYYKNKEMGETFSIVPTCAISGEGIPDLLLLLVQLTQKTMVEKLITYVDKVQCTVLEVKV 269

Query: 1007 VEGHGTTIDVILVNGVLHEGDQIVVCGMQ 1035
            +EG+GTTIDV+LVNG LHEG QIVVCG+Q
Sbjct: 270  MEGYGTTIDVVLVNGELHEGGQIVVCGLQ 297