Cp4.1LG06g05630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g05630
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionGlcNAc kinase
LocationCp4.1LG06: 3411571 .. 3415618 (+)
RNA-Seq ExpressionCp4.1LG06g05630
SyntenyCp4.1LG06g05630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTCCTCTAGAAACTTTAGCAGATGGCTCATTTGCCGGCCGAATCAGAGCCGAACAAAAATTTGTCCGTGATCATCCTCGATTCTTTAGCAAACAAATTCAAGAAGAAATCAAATTAACTATGAACAGCTTCAATTCAAGTTCGAATTCTGTCTCCTCCTCATTGTCGTTTCGTGTTCGTCCTTGCCTTTGCTCTCGCTATCTCTTACTGTAATTTATTTGTAATTTCTTTGTATATATGATCATGAACTCTAGGTGTTTTGACTGCGAGAGTCAATTTTGGTTGTGTTTTTTTAACTTTTGATCCAATTTGTCACTGTGTTGCTCTTGATCGAGGCGGCTGGAGCAGAGATGAAGAGGTGTAGAAATGGCGAACTCTGGGATTTTGAGCACGAGATTCTTGGCGGAGATGATATTATACTTGGAATCGACGGCGGCACCACTTCCACTGTTTGTGTTTGTATTGCTCTTTCCGATCCTCGAGCTATTTCTCCTTCAATGGCTTGTCCTATGCTCGCTCGTGTTGTTGGTGGCTGCTCAAACCATAATAGCGTTGGCGGTACTTCACTATCTTTGTCACTTCTCCCTGATTTAGCTATGTTTTCCGCCATTGCAGCATCAAATTGGACCTTGAATTGACTTTACTTCTTTAAAGAGCCATCGCTTGTATGATCTCGTCCCAAATGGCACAAACGAGTAAACACTTCAGACGATTGAAGGGTTGATTAGAACTCCTTATCGCGTTTTGTTAAGTATCAAATTTAGTTGTCTAGTGTCCGTGTTGATCTCTATTGTAAGCATATCTGATTCCTTCATCATGAATTTGAATGCTAAAAATATGATAACCTTCTGCCATCTCTAGAAACTGCTGCGAGGGAAACACTGGAGCAAGTTATGGCGGAGGCACTTTCAAAGTCTTGTTCAATTCGATCTTCAGTTCGAGCTGTGTGCCTAGCTGTTTCTGGAGTCAACCATCCAACGGATCAAGAAAGAATTCTGAATTGGCTAAGGTATTCATTCTTCTATTAACTGTGAATCCGTAGAGTTTTAAATTTTATTCTTTCTTTTTGTTCGTCATTGGTTAGCCGCTTTCTATTTATACTCTTACCATCCCCTCTAATGGGATTAGGTAATGCGTAGGAATAAAACATGAGCTCAACGAGACTTGATATCATGAACTGCAAATGAACAGGGATATCTTTCCGTGCCATGTAAACCTGTATGTTCAAAATGATGCTGTGGCGGCTCTTGCAAGTGGCACTATGGGAAAGCTCCATGGATGCGTTTTAATTGCTGGAACTGGAACAATTGCATATGGATTCACAGAGGATGGTCGAGAAGCTCGAGCTGCTGGTGCAGGACCAATCTTAGGTGATTGGGGAAGGTGGATTTTTCATTTTGTTCACTAATCCTCTCTCTCTCTCTCTCTCTCACACACACACACTGTTAAAATTCTGGCATAGGTTGGTGTAAACAAAATTGAGGTATAATTATTTAGCCTGATCTAGGAGACCCTTCTGCGCTTTCTAAATTTTCTGATGAACATTTGGTACCAAGTGTAATAGCCCAAGCCTAACCCCACCGGTCACAGACATGGTGCATTTTCTTTGAGTTTTCTCTTTTGGGCTTTTCTCTCAAGATTTTTAAAACGCGTGGAGAGGTTTCCACACCTTTATAAAGGATGTGGGACCTCACAATCCACTCACTTCGGGGTCGAGCGTCCTCGCTAGCACTTGTTCCCCTCTCCAATCGATGTGGGACCCCCCAATCCACCCCTTTGGGGCCTAGCGTCCTCGTGGCACACCGCCTGGTGTCCACCCACTTCGGAGTTTAGACTCCTCACTGGCACATCGATTAGTGTTTGGCTCCGAGTCTCTTATATCATTTGTAACTGCTGAAGCCCACTGGTAACAGATATTGTCTTCTTTGGGTTTTTCTCAAAGTTTACCATTTGTAACAGCTCAATCCCACCACTAACAGATATTATCCTCTTTGGATTTTCCTTATGATTTTTAAAACGTGTCTGCTAGGGAGAGGTTTTCACGCACTTATAAAGAATTCTTCATTCCTCTCTCCAACTAACGTGGGATGTCGAGTAAGTCTGAAAAGTAGTTTAAACATGCCCCTTCCTCTAGAGTCTGGCTAAAGGTCGATGCTCTTGAACAATCTGTTTCCGGTGCTTAATCATTTTATTTTTTTATCTTTCATTCTTTTTTGTAAAAAAGAGTAGTAGTGGGATGAACAGACCCAATATATGTTAATTTTTAACTCTTGATCTATCGCACCACTGTTTGAAAACTGATGTTTATGTTCTTGCAGTGGATATGGGATAGCTGCACAGGCGTTAACCGCAATAATTAGGGCTCATGATGGACGTGGTCCTCATACAATGCTCACTTACAGCATTTTGAAGACACTTGATCTTTCTTCACCAGATGAACTAATAGGGTATGTATTTAAAATACTTAAGATTGCAGTTATATTAAGTCTCATGTTATCTGGTCATTATTTTACCATCAATATTAATATTAGGTGGACATATGCGGATCCATCCTGGGCTCGAATTGCTGCTCTCGTTCCAGTTATTGTAGCATGTGCTGAGGCAGGCGATGAGGTTGCTGACAACATCCTCCTCGACTCAGTTGAAGAATTGGCTTCAAGTGTGAAGGCTGTTATTCAAAGACTCGGCCTGGCTGGTGAAGGTATGAAATCAAACAGTATTCATTATTAGCACTATGTTTATCCCTTGAGATAATTACACTTTGTTTTGCAATCTTCTTCATCTGTTTGAGTTATAATCCCTTGTGCAGATGGACAAGAGGCTTTTCCGCTTGTTATGGTTGGTGGTGTACTCGAAGCAAAAAGAAGGTGGGACATAGCAAAAAAAGTCATAAATTCAATATCCAAAGAATACCCCGGAGTTCTTCCTGTTTGGCCCAAGGTAGTCTTATTTTCCTCTCTTTACTTTGCCTTATTGCAATTGTGGCGTGAGATCCTACGTCGGTTGGAGAGGGGAACGAAACATTCTTTATAAGAGTGTGGAAACCTCTCTCTAGAGACGCGTTTTAAAAACCTTGAGGGGAAAACCCGAAAGGGAAAGCCCAAAAAGAACAATATTTGCTACTGGTAGGCTTGGACTATTACAAATGGTATCAGAGTCAGACACCAGGCGGTGTGCCAACGAGGACGCCAGACTCCCAAAGGGGGTGGATTGTGAGATCCCATATCGACTGGAGAGAGGAACGAGTGCCAGAGAGGACACCGTCCCTAAAAGGGGGGTAAATTGTGAGATCCCCACATTGATTGGAGAGGGAAATGAGTGCCGAAGAGAATGCTAGGCTCCGAAATGAAACATTCTTTAGAAGGGTGTGGAAACCTCTCTCTATCATTTTTAAAAAAAATCTTTGAGAAAAAGTTCAAAGTGGACAATATTTGCTAGCGGTGGGCTTGGATTTCATTCATTTACTCATTAATTTAGAGAATGAGAACCATCTTCTAGATACCCTCGTGTTCGTGAGCTCACAATTGAAGAGAAGCAACCGGTGCCTAAGTGGTTGAGTGTATAGTTATCCCACATCATTTGCTTAAAATGGGAATCAGAAACGAAAGTGATCCCTTTGTTTCTTACGGTTCAAAGTCCTCATTGTTCTTAACAGGTGGAACCGGCACTTGGTGCAGCATTACTAGCCTGGAATTTTTTGAGCAAGGATTATCAGCAGGAAGGAATATAGAGGGTGTTCTTTCTTTAGCTTCACGAGAAAGGGCTGATGAACATGAACTGTACAGTTTGATTGTCAGACCAAACCTAATTAGCGATGTTGGAAAGTTAAGGTAGAAACTTGAAGTAGTCTTAGCTTGTGTTAGTGTCCCAAGGTCTTCGTTGATTTTCATCGTTATTTTAGCTCTTCATGTTAAGATAGTATATATTTTTGAGTGATCCAATCTCTCGTCTGTATGGTAAACTGGATATGGAGGGAAAATCCAATTGAATGAAACCTTCTTTGATCCTCAGAACATTTTAAAATGGTTGGTGTATAAAATATC

mRNA sequence

ATGTCTGCGGCTGGAGCAGAGATGAAGAGGTGTAGAAATGGCGAACTCTGGGATTTTGAGCACGAGATTCTTGGCGGAGATGATATTATACTTGGAATCGACGGCGGCACCACTTCCACTGTTTGTGTTTGTATTGCTCTTTCCGATCCTCGAGCTATTTCTCCTTCAATGGCTTGTCCTATGCTCGCTCGTGTTGTTGGTGGCTGCTCAAACCATAATAGCGTTGGCGAAACTGCTGCGAGGGAAACACTGGAGCAAGTTATGGCGGAGGCACTTTCAAAGTCTTGTTCAATTCGATCTTCAGTTCGAGCTGTGTGCCTAGCTGTTTCTGGAGTCAACCATCCAACGGATCAAGAAAGAATTCTGAATTGGCTAAGGGATATCTTTCCGTGCCATGTAAACCTGTATGTTCAAAATGATGCTGTGGCGGCTCTTGCAAGTGGCACTATGGGAAAGCTCCATGGATGCGTTTTAATTGCTGGAACTGGAACAATTGCATATGGATTCACAGAGGATGGTCGAGAAGCTCGAGCTGCTGGTGCAGGACCAATCTTAGGTGATTGGGGAAGTGGATATGGGATAGCTGCACAGGCGTTAACCGCAATAATTAGGGCTCATGATGGACGTGGTCCTCATACAATGCTCACTTACAGCATTTTGAAGACACTTGATCTTTCTTCACCAGATGAACTAATAGGGTGGACATATGCGGATCCATCCTGGGCTCGAATTGCTGCTCTCGTTCCAGTTATTGTAGCATGTGCTGAGGCAGGCGATGAGGTTGCTGACAACATCCTCCTCGACTCAGTTGAAGAATTGGCTTCAAGTGTGAAGGCTGTTATTCAAAGACTCGGCCTGGCTGGTGAAGATGGACAAGAGGCTTTTCCGCTTGTTATGGTTGGTGGTGTACTCGAAGCAAAAAGAAGGTGGGACATAGCAAAAAAAGTCATAAATTCAATATCCAAAGAATACCCCGGAGTGGAACCGGCACTTGGTGCAGCATTACTAGCCTGGAATTTTTTGAGCAAGGATTATCAGCAGGAAGGAATATAGAGGGTGTTCTTTCTTTAGCTTCACGAGAAAGGGCTGATGAACATGAACTGTACAGTTTGATTGTCAGACCAAACCTAATTAGCGATGTTGGAAAGTTAAGGTAGAAACTTGAAGTAGTCTTAGCTTGTGTTAGTGTCCCAAGGTCTTCGTTGATTTTCATCGTTATTTTAGCTCTTCATGTTAAGATAGTATATATTTTTGAGTGATCCAATCTCTCGTCTGTATGGTAAACTGGATATGGAGGGAAAATCCAATTGAATGAAACCTTCTTTGATCCTCAGAACATTTTAAAATGGTTGGTGTATAAAATATC

Coding sequence (CDS)

ATGTCTGCGGCTGGAGCAGAGATGAAGAGGTGTAGAAATGGCGAACTCTGGGATTTTGAGCACGAGATTCTTGGCGGAGATGATATTATACTTGGAATCGACGGCGGCACCACTTCCACTGTTTGTGTTTGTATTGCTCTTTCCGATCCTCGAGCTATTTCTCCTTCAATGGCTTGTCCTATGCTCGCTCGTGTTGTTGGTGGCTGCTCAAACCATAATAGCGTTGGCGAAACTGCTGCGAGGGAAACACTGGAGCAAGTTATGGCGGAGGCACTTTCAAAGTCTTGTTCAATTCGATCTTCAGTTCGAGCTGTGTGCCTAGCTGTTTCTGGAGTCAACCATCCAACGGATCAAGAAAGAATTCTGAATTGGCTAAGGGATATCTTTCCGTGCCATGTAAACCTGTATGTTCAAAATGATGCTGTGGCGGCTCTTGCAAGTGGCACTATGGGAAAGCTCCATGGATGCGTTTTAATTGCTGGAACTGGAACAATTGCATATGGATTCACAGAGGATGGTCGAGAAGCTCGAGCTGCTGGTGCAGGACCAATCTTAGGTGATTGGGGAAGTGGATATGGGATAGCTGCACAGGCGTTAACCGCAATAATTAGGGCTCATGATGGACGTGGTCCTCATACAATGCTCACTTACAGCATTTTGAAGACACTTGATCTTTCTTCACCAGATGAACTAATAGGGTGGACATATGCGGATCCATCCTGGGCTCGAATTGCTGCTCTCGTTCCAGTTATTGTAGCATGTGCTGAGGCAGGCGATGAGGTTGCTGACAACATCCTCCTCGACTCAGTTGAAGAATTGGCTTCAAGTGTGAAGGCTGTTATTCAAAGACTCGGCCTGGCTGGTGAAGATGGACAAGAGGCTTTTCCGCTTGTTATGGTTGGTGGTGTACTCGAAGCAAAAAGAAGGTGGGACATAGCAAAAAAAGTCATAAATTCAATATCCAAAGAATACCCCGGAGTGGAACCGGCACTTGGTGCAGCATTACTAGCCTGGAATTTTTTGAGCAAGGATTATCAGCAGGAAGGAATATAG

Protein sequence

MSAAGAEMKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAALVPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAKRRWDIAKKVINSISKEYPGVEPALGAALLAWNFLSKDYQQEGI
Homology
BLAST of Cp4.1LG06g05630 vs. ExPASy Swiss-Prot
Match: Q54PM7 (N-acetyl-D-glucosamine kinase OS=Dictyostelium discoideum OX=44689 GN=nagk PE=3 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 3.1e-49
Identity = 120/329 (36.47%), Postives = 183/329 (55.62%), Query Frame = 0

Query: 28  DIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQ- 87
           +I +GIDGG T T  V +  +             LAR    CSN++SVGE  A+  + + 
Sbjct: 4   EIFIGIDGGGTKTSTVAVDSNGQE----------LARHTSPCSNYHSVGEDLAKAAINEG 63

Query: 88  ------VMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFPCHVNLYVQND 147
                  + E ++   +   +V ++CL +SGV+   D+  + +W+ ++    +N  + ND
Sbjct: 64  IKYVIRKVKETITDDDNKEVTVGSICLGMSGVDREKDKLLVKSWVTELLGESINYSIHND 123

Query: 148 AVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALT 207
           A+ AL+SGT GKL G V+I GTG I+ GF  +G   R+ G GP+LGD+GSGY I    L 
Sbjct: 124 AIVALSSGTQGKLFGVVIICGTGCISLGFNREGVSGRSGGWGPLLGDYGSGYQIGYDILR 183

Query: 208 AIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADP---SWARIAALVPVIVACAEA 267
            +++A D  GP T LT  +L+ L L+  ++LI W Y DP   SW + A L P+    A+ 
Sbjct: 184 HVLKAKDQVGPKTSLTQVLLEKLQLTKEEDLISWAY-DPKTQSWQKFAQLSPLAFEQAQL 243

Query: 268 GDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAKRRWDIAKKVI 327
           GDE+++ IL+D+   L   + +VI++LGL   D +E FPLV  GG +E  R+  ++  + 
Sbjct: 244 GDEISNLILVDAANALYDLINSVIKKLGL---DKEEKFPLVYTGGNIE--RKGILSDLLS 303

Query: 328 NSISKEYPGVE-------PALGAALLAWN 340
             I + YP  E       P++GAALLA N
Sbjct: 304 KKIMENYPNAEILNTTCDPSMGAALLALN 316

BLAST of Cp4.1LG06g05630 vs. ExPASy Swiss-Prot
Match: Q97ML3 (N-acetylmuramic acid/N-acetylglucosamine kinase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=murK PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.3e-27
Identity = 101/317 (31.86%), Postives = 157/317 (49.53%), Query Frame = 0

Query: 30  ILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQVMA 89
           ++GIDGG + T      L             +L  V  G SN NS  +   +  L++++ 
Sbjct: 4   VIGIDGGGSKTHMKISTLD----------YKVLLEVFKGPSNINSSTKEEVKRVLQELIM 63

Query: 90  EALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFPCHVNLYVQNDAVAALASGT 149
           E L K         A+C+  +G +   D+  I + +R +      + V NDA  ALA G 
Sbjct: 64  EGLGKLGQSLEECSAICIGTAGADRTEDKSIIEDMIRSL-GYMGKIIVVNDAEIALAGG- 123

Query: 150 MGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALTAIIRAHDGR 209
           + K  G ++I+GTG+I YG  ++GR AR+ G G I+GD GSGY I  +A+ A +++ D R
Sbjct: 124 IEKREGIIVISGTGSICYGRNKEGRSARSGGWGHIIGDEGSGYDIGIKAIKAALKSFDKR 183

Query: 210 GPHTMLTYSILKTLDLSSPDELIGWTY-ADPSWARIAALVPVIVACAEAGDEVADNILLD 269
           G  T+L   IL  L L S ++LI + Y +  +   IA+L  V+ +    GD V+  IL +
Sbjct: 184 GEKTILEGDILDFLKLKSHEDLINYIYRSGVTKKEIASLTRVVNSAYIKGDLVSKRILKE 243

Query: 270 SVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVL-EAKRRWDIAKKVINSISKEYPGV 329
           +  EL  SVKAV++ L +      +   L   GGV+      +D  +K +N     YP V
Sbjct: 244 AARELFLSVKAVVEVLSMQ----NKKVVLTTAGGVINNINYLYDEFRKFLN---LNYPKV 301

Query: 330 -------EPALGAALLA 338
                  + A GA ++A
Sbjct: 304 KIISMKNDSAFGAVIIA 301

BLAST of Cp4.1LG06g05630 vs. ExPASy Swiss-Prot
Match: Q3SZM9 (N-acetyl-D-glucosamine kinase OS=Bos taurus OX=9913 GN=NAGK PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 9.7e-11
Identity = 77/290 (26.55%), Postives = 126/290 (43.45%), Query Frame = 0

Query: 32  GIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQVMAEA 91
           G++GG T +    + LS+   I        LA   G  +NH  +G     E + +++  A
Sbjct: 7   GVEGGGTRS--KVLLLSEDGQI--------LAEADGLSTNHWLIGTDKCVERINEMVNRA 66

Query: 92  LSKS-CSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFPCHVNLY-VQNDAVAALASGT 151
             K+       +R + L++SG +       ++  LRD FP     Y +  DA  ++A+ T
Sbjct: 67  KRKAGVDPLVPLRGLGLSLSGGDQEDAVRMLMEELRDRFPYLSESYLITTDAAGSIATAT 126

Query: 152 MGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALTAIIRAHDG- 211
                G VLI+GTG+       DG E+   G G ++GD GS Y IA QA+  +  + D  
Sbjct: 127 PD--GGVVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNL 186

Query: 212 -RGPHTMLTYSILKTLDLSSPDEL--IGWTYADPSWARIAALVPVIVACAEAGDEVADNI 271
              PH +             PD L  +   Y D   +R A     +   A+ GD ++  I
Sbjct: 187 EAAPHDIGYVKQAMFNYFQVPDRLGILTHLYRDFDKSRFAGFCRKVAEGAQQGDPLSRCI 246

Query: 272 LLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAKRRWDIAKK 316
              + E L   V AV+  +      G+   P++ VG V ++   W++ K+
Sbjct: 247 FRKAGEMLGRHVVAVLPEIDPVLFQGEMGLPILCVGSVWKS---WELLKE 281

BLAST of Cp4.1LG06g05630 vs. ExPASy Swiss-Prot
Match: P81799 (N-acetyl-D-glucosamine kinase OS=Rattus norvegicus OX=10116 GN=Nagk PE=1 SV=4)

HSP 1 Score: 68.6 bits (166), Expect = 1.6e-10
Identity = 76/290 (26.21%), Postives = 127/290 (43.79%), Query Frame = 0

Query: 32  GIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQVMAEA 91
           G++GG T +    + LS+   I        LA   G  +NH  +G     E + +++  A
Sbjct: 7   GVEGGGTRS--KVLLLSEDGQI--------LAEADGLSTNHWLIGTGTCVERINEMVDRA 66

Query: 92  LSKS-CSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFP-CHVNLYVQNDAVAALASGT 151
             K+       +R++ L++SG         ++  LRD FP    + ++  DA  ++A+ T
Sbjct: 67  KRKAGVDPLVPLRSLGLSLSGGEQEDAVRLLMEELRDRFPYLSESYFITTDAAGSIATAT 126

Query: 152 MGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALTAIIRAHDG- 211
                G VLI+GTG+       DG E+   G G ++GD GS Y IA QA+  +  + D  
Sbjct: 127 PD--GGIVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNL 186

Query: 212 -RGPHTMLTYSILKTLDLSSPDEL--IGWTYADPSWARIAALVPVIVACAEAGDEVADNI 271
              PH +             PD L  +   Y D   ++ A     I   A+ GD ++  I
Sbjct: 187 EAAPHDIGHVKQAMFNYFQVPDRLGILTHLYRDFDKSKFAGFCQKIAEGAQQGDPLSRFI 246

Query: 272 LLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAKRRWDIAKK 316
              + E L   V AV+  +      G+   P++ VG V ++   W++ K+
Sbjct: 247 FRKAGEMLGRHVVAVLPEIDPVLFQGELGLPILCVGSVWKS---WELLKE 281

BLAST of Cp4.1LG06g05630 vs. ExPASy Swiss-Prot
Match: Q9UJ70 (N-acetyl-D-glucosamine kinase OS=Homo sapiens OX=9606 GN=NAGK PE=1 SV=4)

HSP 1 Score: 67.0 bits (162), Expect = 4.8e-10
Identity = 75/290 (25.86%), Postives = 125/290 (43.10%), Query Frame = 0

Query: 32  GIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQVMAEA 91
           G++GG T +  + +          S    +LA   G  +NH  +G     E + +++  A
Sbjct: 7   GVEGGGTRSEVLLV----------SEDGKILAEADGLSTNHWLIGTDKCVERINEMVNRA 66

Query: 92  LSKS-CSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFPCHVNLY-VQNDAVAALASGT 151
             K+       +R++ L++SG +       ++  LRD FP     Y +  DA  ++A+ T
Sbjct: 67  KRKAGVDPLVPLRSLGLSLSGGDQEDAGRILIEELRDRFPYLSESYLITTDAAGSIATAT 126

Query: 152 MGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALTAIIRAHDG- 211
                G VLI+GTG+       DG E+   G G ++GD GS Y IA QA+  +  + D  
Sbjct: 127 PD--GGVVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNL 186

Query: 212 -RGPHTMLTYSILKTLDLSSPDEL--IGWTYADPSWARIAALVPVIVACAEAGDEVADNI 271
              PH +             PD L  +   Y D    R A     I   A+ GD ++  I
Sbjct: 187 EAAPHDIGYVKQAMFHYFQVPDRLGILTHLYRDFDKCRFAGFCRKIAEGAQQGDPLSRYI 246

Query: 272 LLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAKRRWDIAKK 316
              + E L   + AV+  +      G+   P++ VG V ++   W++ K+
Sbjct: 247 FRKAGEMLGRHIVAVLPEIDPVLFQGKIGLPILCVGSVWKS---WELLKE 281

BLAST of Cp4.1LG06g05630 vs. NCBI nr
Match: XP_022936045.1 (N-acetyl-D-glucosamine kinase-like [Cucurbita moschata] >XP_022936046.1 N-acetyl-D-glucosamine kinase-like [Cucurbita moschata] >XP_023536637.1 N-acetyl-D-glucosamine kinase-like [Cucurbita pepo subsp. pepo] >XP_023536638.1 N-acetyl-D-glucosamine kinase-like [Cucurbita pepo subsp. pepo] >KAG6591823.1 N-acetyl-D-glucosamine kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 678 bits (1749), Expect = 1.71e-245
Identity = 343/350 (98.00%), Postives = 343/350 (98.00%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPGV-------EPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPGV       EPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGVLPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. NCBI nr
Match: XP_022976806.1 (N-acetyl-D-glucosamine kinase-like [Cucurbita maxima] >XP_022976807.1 N-acetyl-D-glucosamine kinase-like [Cucurbita maxima])

HSP 1 Score: 677 bits (1746), Expect = 4.90e-245
Identity = 342/350 (97.71%), Postives = 343/350 (98.00%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCI+LSDPRAISPSMACPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCISLSDPRAISPSMACPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPGV-------EPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPGV       EPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGVLPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. NCBI nr
Match: XP_008464652.1 (PREDICTED: N-acetyl-D-glucosamine kinase-like [Cucumis melo] >XP_016903225.1 PREDICTED: N-acetyl-D-glucosamine kinase-like [Cucumis melo] >KAA0063348.1 N-acetyl-D-glucosamine kinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 656 bits (1693), Expect = 5.86e-237
Identity = 331/350 (94.57%), Postives = 338/350 (96.57%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPR +SPS++CPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRVVSPSISCPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKS SIRSSVRAVCLAVSGVNHPTDQ+RI +WLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSGSIRSSVRAVCLAVSGVNHPTDQQRISDWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHT LTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTKLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPV+VACAEAGDEVA+NILLDSVEELA SVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVVVACAEAGDEVANNILLDSVEELALSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPG-------VEPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPG       VEPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGILPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. NCBI nr
Match: KAG7024689.1 (N-acetyl-D-glucosamine kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 654 bits (1688), Expect = 1.73e-236
Identity = 332/343 (96.79%), Postives = 332/343 (96.79%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPGVEPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKV           EPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKV-----------EPALGAALLAWNFLSKDYQQEGI 332

BLAST of Cp4.1LG06g05630 vs. NCBI nr
Match: XP_031739612.1 (LOW QUALITY PROTEIN: N-acetyl-D-glucosamine kinase [Cucumis sativus])

HSP 1 Score: 652 bits (1683), Expect = 1.96e-235
Identity = 329/350 (94.00%), Postives = 336/350 (96.00%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRN ELWDFEHEILGGDDIILGIDGGTTSTVCVCI LSDPR +SPSM+CPMLARVVG
Sbjct: 1   MKRCRNDELWDFEHEILGGDDIILGIDGGTTSTVCVCIGLSDPRVVSPSMSCPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKS SIRSSVRAVCLAVSGVNHPTDQ+RIL+WLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSGSIRSSVRAVCLAVSGVNHPTDQQRILDWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDG GPHT LTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGXGPHTKLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPV+VACAEAGDEVA+NILLDSVEELA SV+AVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVVVACAEAGDEVANNILLDSVEELALSVRAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPG-------VEPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPG       VEPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGILPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. ExPASy TrEMBL
Match: A0A6J1F6E8 (GlcNAc kinase OS=Cucurbita moschata OX=3662 GN=LOC111442765 PE=3 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 8.28e-246
Identity = 343/350 (98.00%), Postives = 343/350 (98.00%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPGV-------EPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPGV       EPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGVLPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. ExPASy TrEMBL
Match: A0A6J1IGQ9 (GlcNAc kinase OS=Cucurbita maxima OX=3661 GN=LOC111477073 PE=3 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 2.37e-245
Identity = 342/350 (97.71%), Postives = 343/350 (98.00%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCI+LSDPRAISPSMACPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCISLSDPRAISPSMACPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPGV-------EPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPGV       EPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGVLPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. ExPASy TrEMBL
Match: A0A1S4E5H4 (GlcNAc kinase OS=Cucumis melo OX=3656 GN=LOC103502488 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 2.84e-237
Identity = 331/350 (94.57%), Postives = 338/350 (96.57%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPR +SPS++CPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRVVSPSISCPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKS SIRSSVRAVCLAVSGVNHPTDQ+RI +WLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSGSIRSSVRAVCLAVSGVNHPTDQQRISDWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHT LTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTKLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPV+VACAEAGDEVA+NILLDSVEELA SVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVVVACAEAGDEVANNILLDSVEELALSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPG-------VEPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPG       VEPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGILPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. ExPASy TrEMBL
Match: A0A5A7V6Z0 (GlcNAc kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1505G00190 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 2.84e-237
Identity = 331/350 (94.57%), Postives = 338/350 (96.57%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPR +SPS++CPMLARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRVVSPSISCPMLARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKS SIRSSVRAVCLAVSGVNHPTDQ+RI +WLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSGSIRSSVRAVCLAVSGVNHPTDQQRISDWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD
Sbjct: 121 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGPHT LTYSILKTLDLSSPDELIGWTYADPSWARIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPHTKLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPV+VACAEAGDEVA+NILLDSVEELA SVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVVVACAEAGDEVANNILLDSVEELALSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPG-------VEPALGAALLAWNFLSKDYQQEGI 350
           RRWDIAKKVINSISKEYPG       VEPALGAALLAWNFLSKDYQQEGI
Sbjct: 301 RRWDIAKKVINSISKEYPGILPVWPKVEPALGAALLAWNFLSKDYQQEGI 350

BLAST of Cp4.1LG06g05630 vs. ExPASy TrEMBL
Match: A0A6J1DBA3 (GlcNAc kinase OS=Momordica charantia OX=3673 GN=LOC111019379 PE=3 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 1.48e-229
Identity = 322/350 (92.00%), Postives = 333/350 (95.14%), Query Frame = 0

Query: 8   MKRCRNGELWDFEHEILGGDDIILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVG 67
           MKRCRNGELWDFEHEILGGDD+ILGIDGGTTSTVCV IALS PRA+SPSM+CP+LARVVG
Sbjct: 1   MKRCRNGELWDFEHEILGGDDVILGIDGGTTSTVCVSIALSGPRAVSPSMSCPILARVVG 60

Query: 68  GCSNHNSVGETAARETLEQVMAEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRD 127
           GCSNHNSVGETAARETLEQVMAEALSKS SIRSSVRAVCLAVSGVNHPTDQ+RILNWLRD
Sbjct: 61  GCSNHNSVGETAARETLEQVMAEALSKSGSIRSSVRAVCLAVSGVNHPTDQQRILNWLRD 120

Query: 128 IFPCHVNLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGD 187
           IFPCHV LYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGP LGD
Sbjct: 121 IFPCHVYLYVQNDAVAALASGTMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPTLGD 180

Query: 188 WGSGYGIAAQALTAIIRAHDGRGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAAL 247
           WGSGYGIAAQALTAIIRAHDGRGP T LTYSILKTL LSSPDELIGWTYADPSW+RIAAL
Sbjct: 181 WGSGYGIAAQALTAIIRAHDGRGPLTKLTYSILKTLGLSSPDELIGWTYADPSWSRIAAL 240

Query: 248 VPVIVACAEAGDEVADNILLDSVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 307
           VPV+VACAE GDEVA+NILL+SVEELA SVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK
Sbjct: 241 VPVVVACAETGDEVANNILLESVEELALSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAK 300

Query: 308 RRWDIAKKVINSISKEYPGV-------EPALGAALLAWNFLSKDYQQEGI 350
           +RWDIAKKVINSISKEYPGV       EPALGAALLAWNFLSKDYQQEG+
Sbjct: 301 KRWDIAKKVINSISKEYPGVLPVWPKVEPALGAALLAWNFLSKDYQQEGL 350

BLAST of Cp4.1LG06g05630 vs. TAIR 10
Match: AT1G30540.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 447.6 bits (1150), Expect = 9.3e-126
Identity = 224/321 (69.78%), Postives = 263/321 (81.93%), Query Frame = 0

Query: 29  IILGIDGGTTSTVCVCIALSDPRAISPSMACPMLARVVGGCSNHNSVGETAARETLEQVM 88
           +ILG+DGG TSTVCVC+         P    P+L R V GC+N NSVGETAAR++LEQV+
Sbjct: 31  VILGLDGGATSTVCVCVPFFSFGERFPD-PLPILGRAVAGCTNRNSVGETAARDSLEQVI 90

Query: 89  AEALSKSCSIRSSVRAVCLAVSGVNHPTDQERILNWLRDIFPCHVNLYVQNDAVAALASG 148
           +EAL +S   +S VR VCL VSGVNHP+DQE+I NW+RD+FP HV +YVQNDA+ ALASG
Sbjct: 91  SEALVQSGFDKSDVRGVCLGVSGVNHPSDQEKIENWIRDMFPSHVKVYVQNDAIVALASG 150

Query: 149 TMGKLHGCVLIAGTGTIAYGFTEDGREARAAGAGPILGDWGSGYGIAAQALTAIIRAHDG 208
           TMGKLHGCVLIAGTG IAYGF EDG+EARA+G GPILGDWGSGYGIAAQALTA+IRAHDG
Sbjct: 151 TMGKLHGCVLIAGTGCIAYGFDEDGKEARASGGGPILGDWGSGYGIAAQALTAVIRAHDG 210

Query: 209 RGPHTMLTYSILKTLDLSSPDELIGWTYADPSWARIAALVPVIVACAEAGDEVADNILLD 268
           RGP TMLT +ILK L LSSPDELIGWTYADPSWARIAALVP +V+CAEAGDE++D IL+D
Sbjct: 211 RGPQTMLTSTILKALGLSSPDELIGWTYADPSWARIAALVPQVVSCAEAGDEISDKILVD 270

Query: 269 SVEELASSVKAVIQRLGLAGEDGQEAFPLVMVGGVLEAKRRWDIAKKVINSISKEYPG-- 328
           + E+LA SVKAV+QRLGL G+DG  +FP+VMVGGVL A ++WDI K+V   I++ +PG  
Sbjct: 271 AAEDLALSVKAVVQRLGLCGKDGTASFPVVMVGGVLNANQKWDIGKEVSKRINRYFPGAQ 330

Query: 329 -----VEPALGAALLAWNFLS 343
                VEPA+GAALLA NFLS
Sbjct: 331 TIIPKVEPAVGAALLAMNFLS 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54PM73.1e-4936.47N-acetyl-D-glucosamine kinase OS=Dictyostelium discoideum OX=44689 GN=nagk PE=3 ... [more]
Q97ML33.3e-2731.86N-acetylmuramic acid/N-acetylglucosamine kinase OS=Clostridium acetobutylicum (s... [more]
Q3SZM99.7e-1126.55N-acetyl-D-glucosamine kinase OS=Bos taurus OX=9913 GN=NAGK PE=2 SV=1[more]
P817991.6e-1026.21N-acetyl-D-glucosamine kinase OS=Rattus norvegicus OX=10116 GN=Nagk PE=1 SV=4[more]
Q9UJ704.8e-1025.86N-acetyl-D-glucosamine kinase OS=Homo sapiens OX=9606 GN=NAGK PE=1 SV=4[more]
Match NameE-valueIdentityDescription
XP_022936045.11.71e-24598.00N-acetyl-D-glucosamine kinase-like [Cucurbita moschata] >XP_022936046.1 N-acetyl... [more]
XP_022976806.14.90e-24597.71N-acetyl-D-glucosamine kinase-like [Cucurbita maxima] >XP_022976807.1 N-acetyl-D... [more]
XP_008464652.15.86e-23794.57PREDICTED: N-acetyl-D-glucosamine kinase-like [Cucumis melo] >XP_016903225.1 PRE... [more]
KAG7024689.11.73e-23696.79N-acetyl-D-glucosamine kinase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_031739612.11.96e-23594.00LOW QUALITY PROTEIN: N-acetyl-D-glucosamine kinase [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1F6E88.28e-24698.00GlcNAc kinase OS=Cucurbita moschata OX=3662 GN=LOC111442765 PE=3 SV=1[more]
A0A6J1IGQ92.37e-24597.71GlcNAc kinase OS=Cucurbita maxima OX=3661 GN=LOC111477073 PE=3 SV=1[more]
A0A1S4E5H42.84e-23794.57GlcNAc kinase OS=Cucumis melo OX=3656 GN=LOC103502488 PE=3 SV=1[more]
A0A5A7V6Z02.84e-23794.57GlcNAc kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1505G00190... [more]
A0A6J1DBA31.48e-22992.00GlcNAc kinase OS=Momordica charantia OX=3673 GN=LOC111019379 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30540.19.3e-12669.78Actin-like ATPase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.420.40coord: 151..325
e-value: 1.1E-72
score: 246.9
NoneNo IPR availableGENE3D3.30.420.40coord: 30..338
e-value: 1.1E-72
score: 246.9
NoneNo IPR availablePANTHERPTHR43190N-ACETYL-D-GLUCOSAMINE KINASEcoord: 8..348
NoneNo IPR availablePANTHERPTHR43190:SF9ATPASE, BADF/BADG/BCRA/BCRD TYPE-RELATEDcoord: 8..348
NoneNo IPR availableCDDcd00012NBD_sugar-kinase_HSP70_actincoord: 31..174
e-value: 0.00186073
score: 36.798
IPR002731ATPase, BadF/BadG/BcrA/BcrD typePFAMPF01869BcrAD_BadFGcoord: 31..337
e-value: 2.5E-49
score: 168.2
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 154..344
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 29..149

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g05630.1Cp4.1LG06g05630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046835 carbohydrate phosphorylation
molecular_function GO:0045127 N-acetylglucosamine kinase activity