Cp4.1LG06g05160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g05160
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description(1->3)-beta-glucan endohydrolase
LocationCp4.1LG06: 3085035 .. 3090924 (+)
RNA-Seq ExpressionCp4.1LG06g05160
SyntenyCp4.1LG06g05160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTTAGGCGCGGTCGGAGTCAACTACGGGCTCAACGGCGACAATCTTCCGGTGCCGAGTGAGGTGGTTGAGTTATATGGGCGGTGTGGGATAGACATTTTGCGGGTATTTGAGCCCAATCATTCGGTTCTTGATGCCTTGAAAGGGCGGAGGAATTTAGTGGTGTGGTTGGGAACGAGAAATGAGGATATTCAGGGGTTTGCCGCCGACCAATTAGCGGCGAATTCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGAGTGTTAATATTGCATATATTACTGTCGGTAATGAGGTTGTCCCTGACGATGCGGCGGCGCCATTTGTCGCCAAGGCTATTAAGAATATTATGCAGGCGCTTGTTATTGCCGGCGTCAAAAGCGACATTAAGGTGAGAGTTGAATTTTAGGAATTACTAATCTATGGTATGATATTCCCTATATTAGGGATCATTCCCAAACCCAAACCCATTAACTCACCTAACACCCGTGATCATTCTCTAAATTAGTCAATCGTGGGTGCGACTCCCTAAACCCATGATCATTCTATCGTGGGTGGGACTCCCTAAAACCCATAAATGATCATCCCCTATATTAGCCATCGTGGGATTTTATAAACCCATAAATTCATGATCATTCCCTAAATTAGCCACCGTGGACCGTGAGACTCCCATTCCACCGTGGGACTCTCATACCCATAAACCCATTATCATTCCTTAAATTAGCCACGTCGGACTCCATACCCATAAACTCATGATCATTTCCTAAATTAGCCATTGCGACTCCCATACCTATAAACCCATGATCATGGCCTAAATTAGGCTTAGGCTCAAGTCCCATAGGACTTCCATACCCATAAATCCATGATCCTTCCTAAATTAGGCTTCCATGCCCATAAATGTGTGATCCTTCCTAAATTAGGCTCTCATACCCATAGAAGCGATCCTTCCCAAATTAGGCTCCATGATCCTTCCTAAACTAGGCTCCCATGCCCATAAACCCGATCCTTCCCAAATTAGGCTCCCATGCTCATAAACCCATGATCCTTCCTAAACTAGGCTCCCACGCCCATAAACGCATGATCCTTCCCAAATTAGGTTCCCATGCCCATAAACGCATGATCCTTCCCAAATTAGGTTCCCATGTCCATAAAAAATTAGGTTCCCATGCCCATAAACATGATCCTTCCCAAATTAGGTTCCCATGCCCATAAATGCATCATCCTTCCCAAATTAGGTTCCCATGCCTATAAACCCATGATCCTTCCCATATTAGGTTCCCATGCCCATAAACCCATGATCCTTCCTAAATTAGGCTCCCATGATCCTATCCAAATTAGGCTTCCAGGTCCATAAACCCATGAACCTTCCTTTCCAAACCCATGTCCATAAATCCATGATCATTCTCTAAATGAGGCAAGTTGGTACTTTCTCTAGAATTCTCAACAACAATCCTCCCGGAACAAGGTAAACTATGGAACATCCCAATACTTATAGACCCCTCAAACAGCTTCCCTTCAATCGAGGTCTCGAACAAAGTACACATAACTCTTATGCCAGGTTAGGAATTACAGATCTAACAATGTTATGATATTCTCCACTTTAGAATAAGTTCTCATGACTTATATTGTCTACTTTGAGTATAAATTCCCACCTATAGATCTCCTACCATTACTTATAAACTTACGATCATTCCCTATTTAATTTTGTTGCGACTTTTAGGTGACGACGGTGGTGCCCATGTCAGTAATGGCAGTCTCATACACACCTTCCGCCGGAGCATTCTCTACCACCGCCATCGGACCTTTAAAAGACATCACTCAAGTTTTGGGGACATCCGGCGCACCATTACTGTTGAACGCGTACCCATATTTCGCATACGCATCAAACCCACAACAAATCTCGCTCGATTACGCTCTCTTCAAATCCACCGCGCCGGTGGTGGTGGACGGAAACTTAAAGTACTTCAATCTGTTCGACGCGATGGTGGATTCTTTCTACTCGGCTCTGGAGAAGATCGGCGCGGAGGGAGTGCGAATCGACATCTCGGAGACTGGATGGCCGAGCAAAGGGAACGATCCGTTCACGAGCGTAGATAATGCGGCGACGTATAACAAGAAGTTTGTGGAACATGTGAGGAGCGGCGCCGGAACGCCCAAGAGGCCGAATGTGAAATACGACGGCGTTTTGTTCGAGATGTTCAATGAGGATTTGAAGACGGCGGGGGTTGAGCAGAACTTTGGGATGTTTTATCCCAATATGCATCCGGTTTATCCGTTATGGAATTGCTGAATTATGAATTTATATGTTCGGTTTGGGCTTTTGGCCCAAATTGGGATTCGGTTCACGAAGGCCCATGGGCTTGTCACCCATTGAAAAATCGTTTTAATTAATAAAAATAGCTCAAAAATCTTAAATTAAAAAAAAATCTAAAAGATATCAATCTTTTAAAACATTAATATATATATATATATATANTAAAGTTTAAACATAGTTACCATATTTTTGAAATAAAATATTGATAATCTTCCTAAATAAATTATTATATATATATATATATATATTTAAAANGGAAATTACTGGTATCGCTTAGTTGTAGAGCAGAGGATTGAAAATCTTTGTGTTATCGGTTTAAATTTGCTATTGAAAATACGGGATAAATATATATAGATGAAATTATTAATATTTTGTTTTAAAAATAGTTTTGACTCTGTAAATTATTTTAAAAATATTCACGTTTGACAGTGTTTAAAAATATCTTTCAATTTTTTTATAAAAAAATATATAATTTTCGTTAATTTTAAATGAAATTATTAATATTTTGTTTTGGAGATTAACTTTTATTAATGTTAAAAATATCCTTAAATTCAAAAAAAAAAAAAAAAAAAAAAANATTTTATAAATAGTACTACGGTTAATAAAAATCAAACCTTTTAAAGTTTAAACATAGTTACCATATTTTTGAAATAAAATATTGATAATCTTCCTAAATAAATTATTATATATATATATATATATATTTAAAAATTTAAAGATATTATTCAACCTTAAAAATTTAGTTTTTTTTTTAATTAATTTAGCTAACAAAAAAAAATCAAATTTTCACTATTTAGGACTTTTTGCCTAAACATAATTACTGATATTTTTTAAATGAAATATCGACCATCTTCCCATAAAGTTAATATTATAAATGTCTGCGCTACATTATTGAAATTATCAAGTTATCAATGGAAAGAAATCGAGTTTATTATCTATAATTAATCATAAAATAAATCATATTAATTCATTTTCAAATATAATTCAATTCATAGACACCCATTAGTAATAATTAATTAAACATTTTTCATTAACTCCATAATAAAATAAAAAAATAAAAAAAATAAAAAAAAAAGAAATTAGATTCCCGCTGTGAGGATTATTAACTACGGCACCCATATTAGGCAAATTAAACCCCCTAATTAAACATAATTAGAATGTCCACCTCCTATCTCCCTCTCTAATCCCTTAATTTGAAACCCATTAATAAACAACTAATTTTCTCGTAATCCCCGCCCCTAATTTTTAATTCCCTATAAAATCTCCTCCGCCTTGTTCACCACACCTTACACCACCATGGATTTCTTCCGCCCACCCTCCGCCGCCCTTCTCCTCCTCTTCATCTTCTCCGATCAAGCCCTTCTCATAAGTAATCAATTTTTCGCTTTTTTTTTTTAAATTGTTTTTTTGTTTGTCTTCTCCCTCTTATTTGTTGAGCTTGTGTTCTAGGTGGGTTAGGCGCGGTCGGAGTCAACTACGGGCTCAACGGCGACAATCTTCCGGTGCCGAGTGAGGTGGTTGAGTTATATGGGCGGTGTGGGATAGACATTGTGCGGGTATTTGAGCCCAACCATTGGGTTCTTGATGCGTTGAAAGGGCGGAGGAATTTGGTGGTGTGGTTGGGGACGAGAAATGAGGATATTCAGGGGTTTGCTGCCGACCAATTAGCGGCGAATTCTTGGGTTAATGCTCATGTTGTTCCTTATTACGAGAGTGTTAATATTGCATATATTACTGTCGGTAATGAGGTTGTCCCTGACGATGCGGCGGCGCCATTTGTCGCCAATGCTATTAAGAATATTATGCAGGCGCTTGTTATTGCCGGCGTCAAAAGCGACATTAAGGTGAGGGTTAAATTTTAGGAATTACTAATCTATTCCCTATGTTGGGATCATGGGACTTCAAACCCATAAACCAAGGATCATTCTCGAGACTCTCGAGTACATAAACTCATGATCATTCCCTAAATTAGCCATCGTCCACTCCTAAACCCATGATGATCATTCACTCATACTTAAATCCATAAACCCATGAGGATCATTCCCTAAATTAGTCATGGTGTGACTCCATAAACCCATGATAATCATCTCCTAAAGGAGCCATCGTTGGACTTCCATACCCATAAACTTATGATCGTCATCGTGGATCTTCCATACCCATAAACTCATGATTGCCATCGTGGGTCTTCCATACCCATAAACTTATGATCGCCATCGTGGGTCTTCCATACCCATAAACTCATGATCGCCATTTCCATACCCATAAACTCATGATCATTCATAAATTAGCCATCGTGGGACTCCCATACCCATAAACAACTCATGATCATTCCTAAATTAGCGACTCCCATGCTCATAAACTGAGTATCATTCCTTAAATTAGCCACTCTCATGCCCATAAACTCAGGGTCATTCCTTAAATTAGCCACTCATACCCATAAACCGATGATCATTCCCTAAATTAGCCACCCATACCCATAAACCCATCATCATTCTCTAAATTAGCCACCCATACCCATAAACCCATCATCATTCTCTAAATTAGCCACCCATACCCATAAACCCATCATCATTCTCTAAATTAGCCACCTATTTATAAACCCATGTTCATTTCCTAAATTAGCCAAGTGACTCCATCCCAACAATGTGGGACTCCCTCGGAAATCCTCAACAACAATCCTCTCTACAAAGCATCCCCAATGCTTACAGACCCCTCGAACAACCGCCCTTTAATCGAGGTCTCGAACAAAGTACACCCTTTTTTCGACACTTCACTTTTGACTCCACTCTCGAGGCTCACAATTTTGAATTCTATTGACATAGCTAAGTTGAGGGCATCACTCTAATACCATATTAAGAATTACCGACCTCACACTGGCATGATATTGTCCACTTGAGTATAAGTTCTCATGACTTTGCTACGATATTATCTACTTCAAGTATAAGCTCCCACCAAAAGGTCTACTACCATTCCTTATAAACTCATAATCATTCCCTGCCATTTTTAGGTGACAACGGTGGTGCCCATGACACCACTGGAAGTCTCATACCCACCTTCGGCCGGAGCATTCTCTGCCACCGCCATCGGACCTCTAAAAGACATCACTCGAGTTTTGGGGACATCCGGCGCACCATTACTGTTGAACGTGTACCCATATTTCGCATACGCATCAAACCCACAACAAATCTCGCTCGATTACGCTCTCTTCACATCCACCACGCCGGTGGTGGTGGACGGAAACTTACAGTACTTCAATCTGTTCGACGCGATGGTGGATTCTTTCTACTCGGCGCTGGAAAAGATCGGCGCGGAGGGAGTGCGAATCGACATCTCGGAGACTGGATGGCCGAGCAAAGGGAACGATCCGTTAACGAGCGTTGAAAATGCGGCGACGTATAACAGGAACTTCGTGGAACATGTGAGGAGCGGCGCTGGAACGCCGAGGAGGCCGAATGTGAAATACGACGGCGTTTTGTTCGAGATGTTCAATGAGAATTTGAAGACGGCGGGGGTTGAACAGAACTTTGGGTTGTTTTATTCCAATATGCTTCCGGTTTATTCGTTCTGGAATTGC

mRNA sequence

GTGTTAGGCGCGGTCGGAGTCAACTACGGGCTCAACGGCGACAATCTTCCGGTGCCGAGTGAGGTGGTTGAGTTATATGGGCGGTGTGGGATAGACATTTTGCGGGTATTTGAGCCCAATCATTCGGTTCTTGATGCCTTGAAAGGGCGGAGGAATTTAGTGGTGTGGTTGGGAACGAGAAATGAGGATATTCAGGGGTTTGCCGCCGACCAATTAGCGGCGAATTCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGAGTGTTAATATTGCATATATTACTGTCGGTAATGAGGTTGTCCCTGACGATGCGGCGGCGCCATTTGTCGCCAAGGCTATTAAGAATATTATGCAGGCGCTTGTTATTGCCGGCGTCAAAAGCGACATTAAGTACTTCAATCTGTTCGACGCGATGGTGGATTCTTTCTACTCGGCTCTGGAGAAGATCGGCGCGGAGGGAGTGCGAATCGACATCTCGGAGACTGGATGGCCGAGCAAAGGGAACGATCCGTTCACGAGCGTAGATAATGCGGCGACGTATAACAAGAAGTTTGTGGAACATGTGAGGAGCGGCGCCGGAACGCCCAAGAGGCCGAATGTGAAATACGACGGCGTTTTGTTCGAGATGTTCAATGAGGATTTGAAGACGGCGGGGGTTGAGCAGAACTTTGGGATGTTTTATCCCAATATGCATCCGGCGCGGTCGGAGTCAACTACGGGCTCAACGGCGACAATCTTCCGGTGCCGAGTGAGGTGGTTGAGTTATATGGGCGACATTGTGCGGGTATTTGAGCCCAACCATTGGGTTCTTGATGCGTTGAAAGGGCGGAGGAATTTGGTGGTGTGGTTGGGGACGAGAAATGAGGATATTCAGGGGTTTGCTGCCGACCAATTAGCGGCGAATTCTTGGGTTAATGCTCATGTTGTTCCTTATTACGAGAGTGTTAATATTGCATATATTACTGTCGGTAATGAGGTTGTCCCTGACGATGCGGCGGCGCCATTTGTCGCCAATGCTATTAAGAATATTATGCAGGCGCTTGTTATTGCCGGCGTCAAAAGCGACATTAAGTACTTCAATCTGTTCGACGCGATGGTGGATTCTTTCTACTCGGCGCTGGAAAAGATCGGCGCGGAGGGAGTGCGAATCGACATCTCGGAGACTGGATGGCCGAGCAAAGGGAACGATCCGTTAACGAGCGTTGAAAATGCGGCGACGTATAACAGGAACTTCGTGGAACATGTGAGGAGCGGCGCTGGAACGCCGAGGAGGCCGAATGTGAAATACGACGGCGTTTTGTTCGAGATGTTCAATGAGAATTTGAAGACGGCGGGGGTTGAACAGAACTTTGGGTTGTTTTATTCCAATATGCTTCCGGTTTATTCGTTCTGGAATTGC

Coding sequence (CDS)

GTGTTAGGCGCGGTCGGAGTCAACTACGGGCTCAACGGCGACAATCTTCCGGTGCCGAGTGAGGTGGTTGAGTTATATGGGCGGTGTGGGATAGACATTTTGCGGGTATTTGAGCCCAATCATTCGGTTCTTGATGCCTTGAAAGGGCGGAGGAATTTAGTGGTGTGGTTGGGAACGAGAAATGAGGATATTCAGGGGTTTGCCGCCGACCAATTAGCGGCGAATTCTTGGGTTAATGCTAATGTGGTTCCTTATTACAAGAGTGTTAATATTGCATATATTACTGTCGGTAATGAGGTTGTCCCTGACGATGCGGCGGCGCCATTTGTCGCCAAGGCTATTAAGAATATTATGCAGGCGCTTGTTATTGCCGGCGTCAAAAGCGACATTAAGTACTTCAATCTGTTCGACGCGATGGTGGATTCTTTCTACTCGGCTCTGGAGAAGATCGGCGCGGAGGGAGTGCGAATCGACATCTCGGAGACTGGATGGCCGAGCAAAGGGAACGATCCGTTCACGAGCGTAGATAATGCGGCGACGTATAACAAGAAGTTTGTGGAACATGTGAGGAGCGGCGCCGGAACGCCCAAGAGGCCGAATGTGAAATACGACGGCGTTTTGTTCGAGATGTTCAATGAGGATTTGAAGACGGCGGGGGTTGAGCAGAACTTTGGGATGTTTTATCCCAATATGCATCCGGCGCGGTCGGAGTCAACTACGGGCTCAACGGCGACAATCTTCCGGTGCCGAGTGAGGTGGTTGAGTTATATGGGCGACATTGTGCGGGTATTTGAGCCCAACCATTGGGTTCTTGATGCGTTGAAAGGGCGGAGGAATTTGGTGGTGTGGTTGGGGACGAGAAATGAGGATATTCAGGGGTTTGCTGCCGACCAATTAGCGGCGAATTCTTGGGTTAATGCTCATGTTGTTCCTTATTACGAGAGTGTTAATATTGCATATATTACTGTCGGTAATGAGGTTGTCCCTGACGATGCGGCGGCGCCATTTGTCGCCAATGCTATTAAGAATATTATGCAGGCGCTTGTTATTGCCGGCGTCAAAAGCGACATTAAGTACTTCAATCTGTTCGACGCGATGGTGGATTCTTTCTACTCGGCGCTGGAAAAGATCGGCGCGGAGGGAGTGCGAATCGACATCTCGGAGACTGGATGGCCGAGCAAAGGGAACGATCCGTTAACGAGCGTTGAAAATGCGGCGACGTATAACAGGAACTTCGTGGAACATGTGAGGAGCGGCGCTGGAACGCCGAGGAGGCCGAATGTGAAATACGACGGCGTTTTGTTCGAGATGTTCAATGAGAATTTGAAGACGGCGGGGGTTGAACAGAACTTTGGGTTGTTTTATTCCAATATGCTTCCGGTTTATTCGTTCTGGAATTGC

Protein sequence

VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQALVIAGVKSDIKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGVEQNFGMFYPNMHPARSESTTGSTATIFRCRVRWLSYMGDIVRVFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEVVPDDAAAPFVANAIKNIMQALVIAGVKSDIKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPLTSVENAATYNRNFVEHVRSGAGTPRRPNVKYDGVLFEMFNENLKTAGVEQNFGLFYSNMLPVYSFWNC
Homology
BLAST of Cp4.1LG06g05160 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.3e-47
Identity = 106/304 (34.87%), Postives = 154/304 (50.66%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++G+NYGL GDNLP PS+V+ LY    I  +R+F+PN  VL+AL+G R++ V +G R++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVP--------------------- 123
           +   +A + A   W   N+ PY   +NIA+ITVGNEV+P                     
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSR 161

Query: 124 -------------------DDAAAPFVAKAIKNIMQALVI-------------------- 183
                                +A  F ++A + ++  L +                    
Sbjct: 162 NLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYAS 221

Query: 184 --AGVKSD---------------IKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPS 231
             + ++ D               + Y N+FDA+ D+F  A+EK G + + + +SETGWPS
Sbjct: 222 DPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPS 281

BLAST of Cp4.1LG06g05160 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.0e-47
Identity = 107/304 (35.20%), Postives = 155/304 (50.99%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++G+NYGL GDNLP PS V+ LY   GI  +R+F+PN  VL+AL+G R++ V +G +++D
Sbjct: 33  SIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQD 92

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVP--------------------- 123
           +   AA + A   W  AN+  Y   VNI +ITVGNEV+P                     
Sbjct: 93  LAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSR 152

Query: 124 -------------------DDAAAPFVAKAIKNIMQALVI-------------------- 183
                                +A  F ++A + ++  L +                    
Sbjct: 153 NLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYAS 212

Query: 184 --AGVKSD---------------IKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPS 231
             A ++ D               + Y N+FDA+ D+F  A+EK G + + + +SETGWPS
Sbjct: 213 DPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPS 272

BLAST of Cp4.1LG06g05160 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 4.2e-46
Identity = 106/310 (34.19%), Postives = 150/310 (48.39%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++GV YG+ G+NLP PSEVV LY    I  +R+++PN + L AL+   N+ V L     D
Sbjct: 28  SIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALR-NSNIQVLLDVPRSD 87

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQALVI 123
           +Q  A++  AA  W+  NVV Y+ SV+  YI VGNE++P    A ++  A++NI  AL  
Sbjct: 88  VQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSS 147

Query: 124 AGVKSDIK---------------------------------------------------- 183
           AG+++ IK                                                    
Sbjct: 148 AGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFS 207

Query: 184 ----------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDISETG 234
                                       Y NLFDA+VD+ ++ALE++G   V + +SE+G
Sbjct: 208 YTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESG 267

BLAST of Cp4.1LG06g05160 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-38
Identity = 107/320 (33.44%), Postives = 150/320 (46.88%), Query Frame = 0

Query: 5   VGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNEDI 64
           +GVNYG+ G +LP P +VV LY    I  +R+F P+ +VL+AL+    L V LGT N D+
Sbjct: 7   IGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALR-NSGLGVVLGTLNSDL 66

Query: 65  QGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQALVIA 124
              A+D   A SWV++ V P+  +V+  YI  GNEV+P ++AA  V  A+KN+  AL  A
Sbjct: 67  APLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEAALQAA 126

Query: 125 GVK--------------------------------------------------------- 184
           G+                                                          
Sbjct: 127 GLSVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYPYFAYSA 186

Query: 185 ----------------------SDIKYFNLFDAMVDSFYSALEKI-GAEGVRIDISETGW 244
                                 + ++Y N+FDA++D+ Y+A+EK  G E + + +SETGW
Sbjct: 187 DPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELVVSETGW 246

BLAST of Cp4.1LG06g05160 vs. ExPASy Swiss-Prot
Match: P23547 (Glucan endo-1,3-beta-glucosidase, acidic isoform GI9 OS=Nicotiana tabacum OX=4097 GN=PR2 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.2e-37
Identity = 99/306 (32.35%), Postives = 147/306 (48.04%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++GV YG + +NLP   +V+ LY   GI  +R++ P+ +V +AL+G  N+ + L    +D
Sbjct: 31  SIGVCYGKHANNLPSDQDVINLYNANGIRKMRIYNPDTNVFNALRG-SNIEIILDVPLQD 90

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAA--APFVAKAIKNIMQAL 123
           +Q    D   AN WV  N++ ++  V   YI VGNEV P +    APFVA A++N+  AL
Sbjct: 91  LQSL-TDPSRANGWVQDNIINHFPDVKFKYIAVGNEVSPGNNGQYAPFVAPAMQNVYNAL 150

Query: 124 VIAGVKSDIK-------------------------------------------------- 183
             AG++  IK                                                  
Sbjct: 151 AAAGLQDQIKVSTATYSGILANTYPPKDSIFRGEFNSFINPIIQFLVQHNLPLLANVYPY 210

Query: 184 --------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDISETGWP 231
                                     Y NLFDA++DS Y A+EK G + V I +SE+GWP
Sbjct: 211 FGHIFNTADVPLSYALFTQQEANPAGYQNLFDALLDSMYFAVEKAGGQNVEIIVSESGWP 270

BLAST of Cp4.1LG06g05160 vs. NCBI nr
Match: KAA0064262.1 (putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa])

HSP 1 Score: 638 bits (1645), Expect = 9.14e-223
Identity = 350/640 (54.69%), Postives = 397/640 (62.03%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           V GAVGVNYGLN DNLP PSEV+ LY RCGI+I+R+FEPNH VLDAL+ + NLV+WLGTR
Sbjct: 25  VFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTR 84

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDI+GFA +Q  A++WVNANVVPYYK VNIAYITVGNEVVP DAA PFVA AI+NIMQA
Sbjct: 85  NEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQA 144

Query: 121 LVIAGVKSDIK------------------------------------------------- 180
           LV A V+SDIK                                                 
Sbjct: 145 LVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYP 204

Query: 181 -------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDIS 240
                                          YFNL DAMVDSFY+ALEKIG EGVRI IS
Sbjct: 205 YFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGIS 264

Query: 241 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGV 300
           ETGWPSKGN+PFTSVDNA TYN+ FV+HV SG GTPK PN++YD VLFEMFNEDLK+ GV
Sbjct: 265 ETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVLFEMFNEDLKSPGV 324

Query: 301 EQNFGMFYPNMHPARS--ESTTGSTATIFRCRVRWLSYMGD-----------IVRVFEPN 360
           EQNFG FYPNM+P  S     +G+    +      L    D           IVR+FEPN
Sbjct: 325 EQNFGFFYPNMNPVYSFWNCGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPN 384

Query: 361 HWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEV 420
           H VLDAL+G+++LV+WLGTRNEDI+GFA +QLAAN+WVNA+VVPYY+ VNI+YITVGNEV
Sbjct: 385 HGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEV 444

Query: 421 VPDDAAAPFVANAIKNIMQALVIAGVKSDIK----------------------------- 467
           VP DAAAPFVANAIKNIMQALV  G+KSDIK                             
Sbjct: 445 VPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIM 504

BLAST of Cp4.1LG06g05160 vs. NCBI nr
Match: XP_023521459.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619 bits (1596), Expect = 1.30e-217
Identity = 360/550 (65.45%), Postives = 371/550 (67.45%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR
Sbjct: 26  VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 85

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIK IMQA
Sbjct: 86  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKKIMQA 145

Query: 121 LVIAGVKSDIKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPFTSVDNAAT 180
           LVIAGVKSDIK             SAL                       P+T++D    
Sbjct: 146 LVIAGVKSDIK-----------ISSALF--------------------TTPYTTMD---- 205

Query: 181 YNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAG---VEQNFGMFYPNMHPARSE 240
               F            RP      +LF   ++ L  +G   V  N+G+   N+ P  SE
Sbjct: 206 ----FF-----------RPPSAALLLLFIFSDQALLISGLGAVGVNYGLNGDNL-PVPSE 265

Query: 241 STTGSTATIFRCRVRWLSYMGDIVRVFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAAD 300
                     RC +       DIVRVFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAAD
Sbjct: 266 VVE----LYGRCGI-------DIVRVFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAAD 325

Query: 301 QLAANSWVNAHVVPYYESVNIAYITVGNEVVPDDAAAPFVANAIKNIMQALVIAGVKSDI 360
           QLAANSWVNAHVVPYYESVNIAYITVGNEVVPDDAAAPFVA AIKNIMQALVIAGVKSDI
Sbjct: 326 QLAANSWVNAHVVPYYESVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQALVIAGVKSDI 385

Query: 361 K----------------------------------------------------------- 420
           K                                                           
Sbjct: 386 KVTTVVPMTPLEVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNVYPYFAYASNPQQ 445

Query: 421 ---------------------YFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGND 467
                                YFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGND
Sbjct: 446 ISLDYALFTSTTPVVVDGNLQYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGND 505

BLAST of Cp4.1LG06g05160 vs. NCBI nr
Match: KAE8649635.1 (hypothetical protein Csa_012736 [Cucumis sativus])

HSP 1 Score: 617 bits (1591), Expect = 1.00e-214
Identity = 340/637 (53.38%), Postives = 391/637 (61.38%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           V GAVGVNYGLN DNLP P+EV+ LY RCGI+I+R+FEPNH +L AL G+ NLV+WLGTR
Sbjct: 25  VFGAVGVNYGLNSDNLPKPNEVINLYERCGINIVRIFEPNHEILHALCGKENLVLWLGTR 84

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDI+GFA +Q  AN+WVNANVV YYK VNIAYITVGNEVVP DAA+PFVA AIKN+MQA
Sbjct: 85  NEDIEGFATNQEVANAWVNANVVRYYKDVNIAYITVGNEVVPGDAASPFVANAIKNMMQA 144

Query: 121 LVIAGVKSDIK------------------------------------------------- 180
           L  AGV+SDIK                                                 
Sbjct: 145 LDNAGVQSDIKVTTVVAMTVLEVSSPPSAGAFSAIAARTMKDIGNVLESSCAPILVNVYP 204

Query: 181 -------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDIS 240
                                          YFNL DAMVDSFY+ALEKIG EGVRI IS
Sbjct: 205 YFAYASNPQQISMSYALFTSTSPVVVDGDLQYFNLLDAMVDSFYAALEKIGVEGVRIGIS 264

Query: 241 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGV 300
           ETGWP+KGN+PFTSV+NA TYNK  VEHV SG GTP+ PN++YD VLFEMFNEDLK+ GV
Sbjct: 265 ETGWPTKGNEPFTSVENALTYNKNIVEHVSSGVGTPRMPNLQYDVVLFEMFNEDLKSPGV 324

Query: 301 EQNFGMFYPNMHPARSES---------TTGSTATIF-RCRVRWLSYMGDIVRVFEPNHWV 360
           EQNFG F P+       +         T      ++ RC +       +I+R+FEPNH V
Sbjct: 325 EQNFGFFDPSGFGTLGVNYGLNGDNLPTPSDVINLYGRCGI-------NILRLFEPNHGV 384

Query: 361 LDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEVVPD 420
           LDAL+G+++LV+WLGTRNEDIQGFA +QLAAN+WVNA+VVPYY++VNIAYIT+GNEVVP 
Sbjct: 385 LDALQGKKDLVLWLGTRNEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPG 444

Query: 421 DAAAPFVANAIKNIMQALVIAGVKSDIK-------------------------------- 467
           DAAAPFVAN IKNIMQALV  G+KSDIK                                
Sbjct: 445 DAAAPFVANGIKNIMQALVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDI 504

BLAST of Cp4.1LG06g05160 vs. NCBI nr
Match: XP_016899853.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo])

HSP 1 Score: 590 bits (1522), Expect = 2.69e-204
Identity = 335/642 (52.18%), Postives = 384/642 (59.81%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           V GAVGVNYGLN DNLP PSEV+ LY RCGI+I+R+FEPNH VLDAL+ + NLV+WLGTR
Sbjct: 25  VFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTR 84

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDI+GFA +Q  A++WVNANVVPYYK VNIAYITVGNEVVP DAA PFVA AI+NIMQA
Sbjct: 85  NEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQA 144

Query: 121 LVIAGVKSDIK------------------------------------------------- 180
           LV A V+SDIK                                                 
Sbjct: 145 LVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYP 204

Query: 181 -------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDIS 240
                                          YFNL DAMVDSFY+ALEKIG EGVRI IS
Sbjct: 205 YFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGIS 264

Query: 241 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFE----------- 300
           ETGWPSKGN+PFTSVDNA TYN+ FV+HV SG GTPK PN++YD V F            
Sbjct: 265 ETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVFFSIPFFLLFLCFN 324

Query: 301 ----MFNEDLKTAGVEQNFGMFYPNMHPARSESTTGSTATIFRCRVRWLSYMGDIVRVFE 360
               +F     T GV  N+G+   N+ P  S+          RC +       +IVR+FE
Sbjct: 325 VLNFLFPGGSGTIGV--NYGLNGDNL-PIPSDVIN----LYRRCGI-------NIVRLFE 384

Query: 361 PNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGN 420
           PNH VLDAL+G+++LV+WLGTRNEDI+GFA +QLAAN+WVNA+VVPYY+ VNI+YITVGN
Sbjct: 385 PNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGN 444

Query: 421 EVVPDDAAAPFVANAIKNIMQALVIAGVKSDIK--------------------------- 467
           EVVP DAAAPFVANAIKNIMQALV  G+KSDIK                           
Sbjct: 445 EVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAG 504

BLAST of Cp4.1LG06g05160 vs. NCBI nr
Match: XP_023536146.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439 bits (1130), Expect = 1.84e-149
Identity = 233/313 (74.44%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR
Sbjct: 26  VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 85

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA
Sbjct: 86  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 145

Query: 121 LVIAGVKSDIK------------------------------------------------- 180
           LVIAGVKSDIK                                                 
Sbjct: 146 LVIAGVKSDIKVTTVVPMSVMAVSYTPSAGAFSTTAIGPLKDITQVLGTSGAPLLLNAYP 205

Query: 181 -------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDIS 233
                                          YFNLFDAMVDSFYSALEKIGAEGVRIDIS
Sbjct: 206 YFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDIS 265

BLAST of Cp4.1LG06g05160 vs. ExPASy TrEMBL
Match: A0A5A7V7B6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G002240 PE=3 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 4.43e-223
Identity = 350/640 (54.69%), Postives = 397/640 (62.03%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           V GAVGVNYGLN DNLP PSEV+ LY RCGI+I+R+FEPNH VLDAL+ + NLV+WLGTR
Sbjct: 25  VFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTR 84

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDI+GFA +Q  A++WVNANVVPYYK VNIAYITVGNEVVP DAA PFVA AI+NIMQA
Sbjct: 85  NEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQA 144

Query: 121 LVIAGVKSDIK------------------------------------------------- 180
           LV A V+SDIK                                                 
Sbjct: 145 LVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYP 204

Query: 181 -------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDIS 240
                                          YFNL DAMVDSFY+ALEKIG EGVRI IS
Sbjct: 205 YFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGIS 264

Query: 241 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGV 300
           ETGWPSKGN+PFTSVDNA TYN+ FV+HV SG GTPK PN++YD VLFEMFNEDLK+ GV
Sbjct: 265 ETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVLFEMFNEDLKSPGV 324

Query: 301 EQNFGMFYPNMHPARS--ESTTGSTATIFRCRVRWLSYMGD-----------IVRVFEPN 360
           EQNFG FYPNM+P  S     +G+    +      L    D           IVR+FEPN
Sbjct: 325 EQNFGFFYPNMNPVYSFWNCGSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPN 384

Query: 361 HWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEV 420
           H VLDAL+G+++LV+WLGTRNEDI+GFA +QLAAN+WVNA+VVPYY+ VNI+YITVGNEV
Sbjct: 385 HGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEV 444

Query: 421 VPDDAAAPFVANAIKNIMQALVIAGVKSDIK----------------------------- 467
           VP DAAAPFVANAIKNIMQALV  G+KSDIK                             
Sbjct: 445 VPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIM 504

BLAST of Cp4.1LG06g05160 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 590 bits (1522), Expect = 1.30e-204
Identity = 335/642 (52.18%), Postives = 384/642 (59.81%), Query Frame = 0

Query: 1   VLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTR 60
           V GAVGVNYGLN DNLP PSEV+ LY RCGI+I+R+FEPNH VLDAL+ + NLV+WLGTR
Sbjct: 25  VFGAVGVNYGLNSDNLPKPSEVINLYERCGINIVRIFEPNHEVLDALRCKENLVLWLGTR 84

Query: 61  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQA 120
           NEDI+GFA +Q  A++WVNANVVPYYK VNIAYITVGNEVVP DAA PFVA AI+NIMQA
Sbjct: 85  NEDIKGFATNQEVADAWVNANVVPYYKDVNIAYITVGNEVVPGDAATPFVANAIQNIMQA 144

Query: 121 LVIAGVKSDIK------------------------------------------------- 180
           LV A V+SDIK                                                 
Sbjct: 145 LVNADVQSDIKVTTVVAMTALEVSSPPSAGAFTAIAARAMKDIGSVLESSCAPILMNVYP 204

Query: 181 -------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDIS 240
                                          YFNL DAMVDSFY+ALEKIG EGVRI IS
Sbjct: 205 YFAYASNPQQIPLSYALFTSTTPVVVDGDLEYFNLLDAMVDSFYTALEKIGVEGVRIGIS 264

Query: 241 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFE----------- 300
           ETGWPSKGN+PFTSVDNA TYN+ FV+HV SG GTPK PN++YD V F            
Sbjct: 265 ETGWPSKGNEPFTSVDNALTYNQNFVKHVSSGVGTPKMPNLQYDIVFFSIPFFLLFLCFN 324

Query: 301 ----MFNEDLKTAGVEQNFGMFYPNMHPARSESTTGSTATIFRCRVRWLSYMGDIVRVFE 360
               +F     T GV  N+G+   N+ P  S+          RC +       +IVR+FE
Sbjct: 325 VLNFLFPGGSGTIGV--NYGLNGDNL-PIPSDVIN----LYRRCGI-------NIVRLFE 384

Query: 361 PNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGN 420
           PNH VLDAL+G+++LV+WLGTRNEDI+GFA +QLAAN+WVNA+VVPYY+ VNI+YITVGN
Sbjct: 385 PNHGVLDALQGKKDLVLWLGTRNEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGN 444

Query: 421 EVVPDDAAAPFVANAIKNIMQALVIAGVKSDIK--------------------------- 467
           EVVP DAAAPFVANAIKNIMQALV  G+KSDIK                           
Sbjct: 445 EVVPGDAAAPFVANAIKNIMQALVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAG 504

BLAST of Cp4.1LG06g05160 vs. ExPASy TrEMBL
Match: A0A6J1IM63 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111476518 PE=3 SV=1)

HSP 1 Score: 431 bits (1109), Expect = 1.36e-146
Identity = 228/312 (73.08%), Postives = 231/312 (74.04%), Query Frame = 0

Query: 2   LGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRN 61
           LGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRV+EPNHSVLDALKGRRNLVVWLGTRN
Sbjct: 27  LGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTRN 86

Query: 62  EDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQAL 121
           EDI+GFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVA AIKNIMQAL
Sbjct: 87  EDIKGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNIMQAL 146

Query: 122 VIAGVKSDIK-------------------------------------------------- 181
           VIAGVKSDIK                                                  
Sbjct: 147 VIAGVKSDIKVSTVVPMSALAVSHPPSAGAFSTTAIGPLKDITLVLGTSGAPLLLNAYTY 206

Query: 182 ------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDISE 233
                                         YFNLFDAMVDSFYSALEKIGAEGVRIDISE
Sbjct: 207 FAYASNPQQISLDYALFTSTAPVVVDGNLQYFNLFDAMVDSFYSALEKIGAEGVRIDISE 266

BLAST of Cp4.1LG06g05160 vs. ExPASy TrEMBL
Match: A0A6J1FC84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 PE=3 SV=1)

HSP 1 Score: 429 bits (1104), Expect = 7.80e-146
Identity = 228/311 (73.31%), Postives = 229/311 (73.63%), Query Frame = 0

Query: 2   LGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRN 61
           LGAVGVNYGLNGDNLPVPSEVV LYGRCGIDILRV+EPNHSVLDALKGRRNLVVWLGTRN
Sbjct: 27  LGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTRN 86

Query: 62  EDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQAL 121
           EDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVA AIKNIMQAL
Sbjct: 87  EDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNIMQAL 146

Query: 122 VIAGVKSDIK-------------------------------------------------- 181
           VIAGVKSDIK                                                  
Sbjct: 147 VIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNAYPY 206

Query: 182 ------------------------------YFNLFDAMVDSFYSALEKIGAEGVRIDISE 232
                                         YFNLFDAMVDSFYSALEKIGAEGVRIDISE
Sbjct: 207 FAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDISE 266

BLAST of Cp4.1LG06g05160 vs. ExPASy TrEMBL
Match: A0A6J1F682 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442719 PE=3 SV=1)

HSP 1 Score: 407 bits (1045), Expect = 6.77e-137
Identity = 249/466 (53.43%), Postives = 269/466 (57.73%), Query Frame = 0

Query: 2   LGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRN 61
           LGAVGVNYGLNGDNLPV S+VVELYGRCGIDI+RVFEPNH VLDALKGRRNLVVWLGTRN
Sbjct: 27  LGAVGVNYGLNGDNLPVASDVVELYGRCGIDIVRVFEPNHWVLDALKGRRNLVVWLGTRN 86

Query: 62  EDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQAL 121
           EDIQGFAADQLAANSWVNA+VVPYY+SVNIAYITVGNEVVP DAAAPFVA AIKNIMQAL
Sbjct: 87  EDIQGFAADQLAANSWVNAHVVPYYESVNIAYITVGNEVVPGDAAAPFVANAIKNIMQAL 146

Query: 122 VIAGVKSDIKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPFTSVDNAATY 181
           VIAGVKSDIK   +                     +++S    PS G    T++      
Sbjct: 147 VIAGVKSDIKVTTVVPM----------------TPLEVSYP--PSAGAFSATAIGPL--- 206

Query: 182 NKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGVEQNFGMFYPNMHPARSESTTG 241
             K +  V   +G P   NV                          YP            
Sbjct: 207 --KDITQVLGTSGAPLLLNV--------------------------YP------------ 266

Query: 242 STATIFRCRVRWLSYMGDIVRVFEPNHWVLDALKGRRNLVVWLGTRNEDIQGFAADQLAA 301
                      + +Y  +                                          
Sbjct: 267 -----------YFAYASN------------------------------------------ 326

Query: 302 NSWVNAHVVPYYESVNIAYITVGNEVVPDDAAAPFVANAIKNIMQALVIAGVKSDIKYFN 361
                    P   S++ A  T    VV D                         +++YFN
Sbjct: 327 ---------PQQISLDYALFTSTTPVVVD------------------------GNLQYFN 345

Query: 362 LFDAMVDSFYSALEKIGAEGVRIDISETGWPSKGNDPLTSVENAATYNRNFVEHVRSGAG 421
           LFDAMVDSFYSALEKIGAE VRIDISETGWPSKGND LTSVENAATYNRNFVEHVRSGAG
Sbjct: 387 LFDAMVDSFYSALEKIGAERVRIDISETGWPSKGNDQLTSVENAATYNRNFVEHVRSGAG 345

Query: 422 TPRRPNVKYDGVLFEMFNENLKTAGVEQNFGLFYSNMLPVYSFWNC 467
           TPRRPNVKYDGVLFEMFNENLKTAGVEQNFGLFY NMLPVYSFWNC
Sbjct: 447 TPRRPNVKYDGVLFEMFNENLKTAGVEQNFGLFYPNMLPVYSFWNC 345

BLAST of Cp4.1LG06g05160 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 191.4 bits (485), Expect = 1.6e-48
Identity = 106/304 (34.87%), Postives = 154/304 (50.66%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++G+NYGL GDNLP PS+V+ LY    I  +R+F+PN  VL+AL+G R++ V +G R++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVP--------------------- 123
           +   +A + A   W   N+ PY   +NIA+ITVGNEV+P                     
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSR 161

Query: 124 -------------------DDAAAPFVAKAIKNIMQALVI-------------------- 183
                                +A  F ++A + ++  L +                    
Sbjct: 162 NLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYAS 221

Query: 184 --AGVKSD---------------IKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPS 231
             + ++ D               + Y N+FDA+ D+F  A+EK G + + + +SETGWPS
Sbjct: 222 DPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPS 281

BLAST of Cp4.1LG06g05160 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 190.3 bits (482), Expect = 3.6e-48
Identity = 107/304 (35.20%), Postives = 155/304 (50.99%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++G+NYGL GDNLP PS V+ LY   GI  +R+F+PN  VL+AL+G R++ V +G +++D
Sbjct: 33  SIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQD 92

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVP--------------------- 123
           +   AA + A   W  AN+  Y   VNI +ITVGNEV+P                     
Sbjct: 93  LAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSR 152

Query: 124 -------------------DDAAAPFVAKAIKNIMQALVI-------------------- 183
                                +A  F ++A + ++  L +                    
Sbjct: 153 NLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYAS 212

Query: 184 --AGVKSD---------------IKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPS 231
             A ++ D               + Y N+FDA+ D+F  A+EK G + + + +SETGWPS
Sbjct: 213 DPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPS 272

BLAST of Cp4.1LG06g05160 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 2.0e-46
Identity = 105/303 (34.65%), Postives = 148/303 (48.84%), Query Frame = 0

Query: 5   VGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNEDI 64
           VG+NYGL GDNLP PS V++ Y    +  +R+FEPN  VL+AL+G R++ V +G +NED+
Sbjct: 34  VGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNEDL 93

Query: 65  QGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQ----- 124
           +  AA++ A  SW + N+ PY   VNI +ITVGN+ +P D   P V   I+++       
Sbjct: 94  EALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKSR 153

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 154 NLQISISTTVTTTSLARLKPPSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPYYFHAS 213

Query: 185 -----ALVIAGVKSD--------IKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPS 230
                 L  A   +D        +KY NLFDA+ D+F  A+EK G +G+ + +SETGWPS
Sbjct: 214 DPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSETGWPS 273

BLAST of Cp4.1LG06g05160 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 5.3e-44
Identity = 101/295 (34.24%), Postives = 143/295 (48.47%), Query Frame = 0

Query: 4   AVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNED 63
           ++G+NYGL GDN P PS V+ LY   GI  +R+F+P   VL+AL+G RN+ V +G R++D
Sbjct: 33  SIGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQD 92

Query: 64  IQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVP--------------------- 123
           +   +A++ A   W   N+ PY   VNIA+ITVGNEV+P                     
Sbjct: 93  LGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGPIGPQVLPVMQSLTILVKSM 152

Query: 124 -------------------DDAAAPFVAKAIKNIMQALVIAGVKS--------------- 183
                                +A  F ++A + ++  L +    S               
Sbjct: 153 NLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPYAS 212

Query: 184 ----------------------DIKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPS 222
                                  + Y N+FDA+ D+F  A+EK G + + + +SETGWPS
Sbjct: 213 DPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPS 272

BLAST of Cp4.1LG06g05160 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 7.7e-43
Identity = 99/302 (32.78%), Postives = 144/302 (47.68%), Query Frame = 0

Query: 5   VGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRNEDI 64
           +G+NYGL GDNLP PSEV+ LY    +  +R+F+    VL+A +G RN+ V +  +N+D+
Sbjct: 33  IGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRNIGVMVDVKNQDL 92

Query: 65  QGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPF--VAKAIKNIMQA-- 124
           +  +  + A N+W   N+ PY   VNI +I VGNEV+P +  +    V K++ NI+++  
Sbjct: 93  EALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGEIGSYVLPVMKSLTNIVKSRS 152

Query: 125 ---LVIAGV--------------------------------------------------- 184
              L+   V                                                   
Sbjct: 153 LPILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPYFAYAAD 212

Query: 185 -------------------KSDIKYFNLFDAMVDSFYSALEKIGAEGVRIDISETGWPSK 230
                                 + Y N+FD + D+F  A+EK G + + + ++ETGWPS 
Sbjct: 213 PINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAFVWAMEKEGVKDLPMVVTETGWPSA 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O493532.3e-4734.87Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN... [more]
O493525.0e-4735.20Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O223174.2e-4634.19Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2[more]
Q024391.5e-3833.44Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4... [more]
P235471.2e-3732.35Glucan endo-1,3-beta-glucosidase, acidic isoform GI9 OS=Nicotiana tabacum OX=409... [more]
Match NameE-valueIdentityDescription
KAA0064262.19.14e-22354.69putative glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo var. makuwa][more]
XP_023521459.11.30e-21765.45probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo][more]
KAE8649635.11.00e-21453.38hypothetical protein Csa_012736 [Cucumis sativus][more]
XP_016899853.12.69e-20452.18PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo][more]
XP_023536146.11.84e-14974.44probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5A7V7B64.43e-22354.69(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S4DV561.30e-20452.18(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV... [more]
A0A6J1IM631.36e-14673.08(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111476518 PE=... [more]
A0A6J1FC847.80e-14673.31(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 P... [more]
A0A6J1F6826.77e-13753.43(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442719 P... [more]
Match NameE-valueIdentityDescription
AT5G20340.11.6e-4834.87beta-1,3-glucanase 5 [more]
AT5G20330.13.6e-4835.20beta-1,3-glucanase 4 [more]
AT5G20560.12.0e-4634.65Glycosyl hydrolase superfamily protein [more]
AT1G33220.15.3e-4434.24Glycosyl hydrolase superfamily protein [more]
AT5G20390.17.7e-4332.78Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 255..356
e-value: 2.5E-24
score: 87.8
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 357..466
e-value: 4.3E-41
score: 142.9
coord: 4..129
e-value: 1.1E-35
score: 125.1
coord: 130..239
e-value: 8.9E-39
score: 135.3
NoneNo IPR availablePANTHERPTHR32227:SF305GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATEDcoord: 3..119
NoneNo IPR availablePANTHERPTHR32227:SF305GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATEDcoord: 261..345
coord: 128..233
coord: 355..463
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 356..463
e-value: 3.0E-32
score: 112.5
coord: 129..233
e-value: 2.1E-29
score: 103.1
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 3..119
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 261..345
coord: 128..233
coord: 355..463
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 5..234
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 252..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g05160.1Cp4.1LG06g05160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds