Cp4.1LG06g04710 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG06g04710 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 271.6 bits (693), Expect = 2.4e-71
Identity = 222/703 (31.58%), Postives = 327/703 (46.51%), Query Frame = 0

Query: 21  IVAVAI-DRDKGSQGALKWAIDYLLQKGQSVF-LIHVKLRSPSNSSQKMSS------KSD 80
           IVAVAI   +  ++G ++WA+     +   VF L+HV+ R  ++ S           K D
Sbjct: 7   IVAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRKDLTTSVYKKD 66

Query: 81  SEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENITNDVPGSVV-- 140
            +   +E+ LP R     +++Q   +VLE  D A AI +   +  I  +      S++  
Sbjct: 67  VDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFS 126

Query: 141 -------------KGAPDFCNVFVISKGKVQSMRSASRPAPV-ISPLRGQLAQQSSTKSD 200
                           P FC+V VISKGK+ ++R +       I+  R +    S + S 
Sbjct: 127 WKLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSSDSHSG 186

Query: 201 TPEIQMTHSASARDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDF 260
           T     +H  S+  L  Q        + +   +    +         R+    V  S   
Sbjct: 187 TVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRAGSLDVDESKLL 246

Query: 261 DLPHSFESLQMGRMSADMNFSS-------EFSSISQDSDRLSISSQSMEDVEAEMRRLKL 320
           +    + +   G      + SS       E SS S  SD  S SSQ  +D E E  +LK+
Sbjct: 247 NQKGFYRTSSSGIGYGGSDISSWRSSQMEEASSSSTYSDPTSSSSQIHKDFELE--KLKI 306

Query: 321 ELKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKA 380
           EL+    MY+ A  E + A +K  +L + + EE  RL+   +  E A  + E E+ + + 
Sbjct: 307 ELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEADEVVEMERERQED 366

Query: 381 AIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDI---RYRKYTIDEIEV 440
           A   AE  R   E E ++R++AE +A +  +EK++  DAL    +   +Y K+  +EI  
Sbjct: 367 AENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVE 426

Query: 441 ATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQ------------- 500
           AT  F++  KIG GGYG VYRC+L HT VA+KVL  D +    QF Q             
Sbjct: 427 ATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHL 486

Query: 501 ------------------------------------------------------------ 560
                                                                       
Sbjct: 487 LLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYF 546

Query: 561 ----EPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCY 611
               EP P+VHRDLKPANILLDRN VSKI DVGL+++V    +++ T +  T   GTF Y
Sbjct: 547 LHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFY 606

BLAST of Cp4.1LG06g04710 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 4.1e-71
Identity = 220/751 (29.29%), Postives = 341/751 (45.41%), Query Frame = 0

Query: 22  VAVAIDRDKGSQGALKWAIDYLLQKGQSVF-LIHVK-----LRSPSNSSQKMSS------ 81
           VAVAI+  K S+  + WA++  + +G + F L++V+     + +P   +  +S       
Sbjct: 24  VAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDVV 83

Query: 82  ---KSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIT---- 141
              K + + S  E+  P++    R+ +Q   ++L+  + A AI E  A   +  +     
Sbjct: 84  SAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIGMS 143

Query: 142 --------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLAQQSSTK 201
                    D+   +    P FC V+VISKGK+ S+R +   A     +R + +  +S  
Sbjct: 144 LRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDAS--GSIRFERSSSTSGS 203

Query: 202 SDTPEIQMTHSASARDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSST 261
           +D+P +           P  +  +S VS  +  +    P+L  S  +   +     SS T
Sbjct: 204 TDSPRLP----------PEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGT 263

Query: 262 DFDLPHSFESLQM------GRMSADMNFSSEF---------------------------S 321
           D +   +   +++      G+ + D +FS+ F                           S
Sbjct: 264 DQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSS 323

Query: 322 SISQDSDRLS-------------------------ISSQSMED----VEAEMRRLKLELK 381
           S S + D ++                         +S  S+ D    +  E+ +L+ ELK
Sbjct: 324 SSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELK 383

Query: 382 QTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIE 441
              EMY+ A  E + A +K  EL + + EE ++L E +   E A   A +EK + + A++
Sbjct: 384 HVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMK 443

Query: 442 AAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFA 501
            AE  + +   E   R +AE KA ++A EK K   +L +  ++Y+ YT +EI  AT  FA
Sbjct: 444 EAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFA 503

Query: 502 ESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE------------------ 561
           E+ KIG G YG VY+C+L HT  A+KVL    TQ   QF QE                  
Sbjct: 504 ENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGA 563

Query: 562 -----------------------------------------------------PEPLVHR 612
                                                                P P++HR
Sbjct: 564 CPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHR 623

BLAST of Cp4.1LG06g04710 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 261.9 bits (668), Expect = 1.9e-68
Identity = 224/732 (30.60%), Postives = 325/732 (44.40%), Query Frame = 0

Query: 22  VAVAIDRDKGSQGALKWAIDYLLQKGQSVF-LIHV-----KLRSPSNSSQKMSSKSDSEA 81
           V VA+     S+  + WAI+    +G   F L+H+      + +P  ++  +S   D   
Sbjct: 22  VVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVV 81

Query: 82  SI---------KELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIT---- 141
           +          +E+  P+     R+ +    +V+E  + A AI E     +I+ I     
Sbjct: 82  TAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGS 141

Query: 142 --------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLAQQSSTK 201
                    D+   +    P+FC V+V+SKGK+  +R +           G     SS+ 
Sbjct: 142 SRSFFSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSG 201

Query: 202 SDTPE------IQMTHSASARDL-------------------PTQESDISFVSSGRPSID 261
           S  P       +   H + +R L                   P + S +    +   S+D
Sbjct: 202 SSGPTSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLD 261

Query: 262 ----RIFPSL-YDSQDTGGRSTP----------PQVSSSTDFDLPHSFESLQMGRMSADM 321
               R   S+   S DT  R TP             SSS++ +  +         M  D 
Sbjct: 262 AEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDT 321

Query: 322 NFSSEFSSISQDSDRLSISSQSMEDVEA--EMRRLKLELKQTMEMYSTACKEALTAKQKT 381
             S      S  SD LS  S +   V    E+ +L+ EL+   EMY+ A  E   A +K 
Sbjct: 322 THSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKL 381

Query: 382 VELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQKRMKAE 441
            EL + +LEE  +LEE +L    A  LAE+EK   + A   AE+ R  AE E  +R +AE
Sbjct: 382 GELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAE 441

Query: 442 MKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPVYRCDLDH 501
            K+ ++ +EK+K    L +  ++Y+ +  +EI  AT  F+E  KIG G YG VY+C+L H
Sbjct: 442 RKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHH 501

Query: 502 TPVAIKVLRPDATQGRSQFKQE-------------------------------------- 561
           T   +KVL+    Q   QF+QE                                      
Sbjct: 502 TTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDR 561

Query: 562 ---------------------------------PEPLVHRDLKPANILLDRNFVSKISDV 612
                                            P+P++HRDLKPANILLD NFVSK+ DV
Sbjct: 562 LFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDV 621

BLAST of Cp4.1LG06g04710 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.5e-68
Identity = 217/699 (31.04%), Postives = 322/699 (46.07%), Query Frame = 0

Query: 18  VKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHV-----KLRSPS----------- 77
           VKG++   +    GS+ A++WA+D LL K     +IHV      + +P+           
Sbjct: 28  VKGLIGDKLG-GAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTRMWV 87

Query: 78  NSSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDV-VLEDTDAAKAIIEYAANKAIEN 137
            ++       + E S+ E+++       +K+ + + V  L+   + ++   Y  ++  + 
Sbjct: 88  VTAGDRLPVEEVEESVVEMYVR----DVKKEYETVFVPFLKMCKSTRSTKRYFRSRRTKG 147

Query: 138 ITNDVPGSVVKGAPDFCNVFVISKGKV--QSMRSASRPAPVISP---------LRGQLAQ 197
               VP +V++ AP+ C V+++ K ++  +SM       P  SP         LR   A 
Sbjct: 148 --TGVPLTVLRYAPETCEVYIVCKDRITTKSMDPLINREPCTSPHAAATAHDFLRDWAAS 207

Query: 198 QSSTKSDT-------PEIQMTHSASARDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTG 257
             + +S T        E     SASAR+L  +   ++             P    S    
Sbjct: 208 FHTLRSPTLPDPRQSTEAGTRRSASARELRFEALSLTCNK----------PKTPQSSKAS 267

Query: 258 GRSTPPQVSSSTDFDLPH----SFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSME 317
             +TP         D+P      F+       S   N  SE     +     S  S+ +E
Sbjct: 268 SATTPEIFRRRRGSDIPQLNYSDFDKTCTKPQSNVENIVSEHRDSDRSPPETSRKSKKVE 327

Query: 318 DVEAEMRRLKLELKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALA 377
            +E E+ RLK EL+ T+  Y  AC+E  + + K   L    L E +R+  A    E    
Sbjct: 328 -IEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRVNNAVEKEELQRN 387

Query: 378 LAEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRK 437
            A  EK +   A++  E A+ +   E  +R  AE+ AL+   EKKK +D L   D RYRK
Sbjct: 388 TAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLLGTDHRYRK 447

Query: 438 YTIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE---- 497
           YTI+EI  ATE F+  + IGEGGYG VY+C LD TP A+KV+R D  + + +F +E    
Sbjct: 448 YTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQEFLKEVEVL 507

Query: 498 ------------------------------------------------------------ 557
                                                                       
Sbjct: 508 SQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRVIFEVACGL 567

Query: 558 -------PEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTF 607
                  PEP+VHRDLKP NILL+RN+VSKI+DVGLA+LV     ++VT YR +  AGT 
Sbjct: 568 AFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYRNSVLAGTL 627

BLAST of Cp4.1LG06g04710 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.1e-55
Identity = 208/725 (28.69%), Postives = 313/725 (43.17%), Query Frame = 0

Query: 22  VAVAIDRDKGSQGALKWAID-YLLQKGQSVFLIHV--KLRSPSNSSQKMSSKSDS----- 81
           VA+AI     S+  +KWA++ +   K  +  LIH+  K+ +   +S  + S S+      
Sbjct: 34  VALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISEELEEVA 93

Query: 82  --------EASIKELFLPFRCFCTRKDIQCMDV--------------VLEDTDAAKAIIE 141
                   + + + L  PF+  C RK ++  +               VLE    A AI +
Sbjct: 94  AAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSVAVAITK 153

Query: 142 YAANKAIENI------------TNDVPGSVVKGAPDFCNVFVISKGKVQSM--------R 201
                 I N+              D+  S+     + C V+V+S G V  +        R
Sbjct: 154 EVNQHLISNLIIGRSSQAASSRNYDITASISASVSNLCTVYVVSNGGVHILAKDTSDTER 213

Query: 202 SASRPAPVISPLRGQLAQQSSTKSDTPEIQM---THSAS---ARDLPTQESDISF-VSSG 261
           + +             +  S   SD     +    H+ S    ++LPT    +S  + + 
Sbjct: 214 NDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPTIVRGVSVPMETS 273

Query: 262 RPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLP-HSFESLQMGRMSADMNFSSEFSSI 321
               D       D+ +   + + P+ S S  ++     F+  +    S   NF  E+ ++
Sbjct: 274 STESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKDAMSSMSSNF--EYGNV 333

Query: 322 SQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQKTVELQRWKLEEE 381
                     +Q   D   E+ +L+ EL+   EMY+ A  E L A +K  EL   K EE 
Sbjct: 334 VTPLGHYFTDNQ---DTLNEISKLRAELRHAHEMYAVAQVETLDASRKLNEL---KFEEL 393

Query: 382 QRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKK 441
             LE         +A  E EK + K          R  E E  +R +AEMKA  EA+EK+
Sbjct: 394 TLLEHE----TKGIAKKETEKFEQK----------RREEREAAQRREAEMKATHEAKEKE 453

Query: 442 KAVD-ALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRP 501
           K  + +L    ++Y+++T +EI  AT  F+E  KIG G YG VY+C+L HT  A+KVL  
Sbjct: 454 KLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHS 513

Query: 502 DATQGRSQFKQE------------------------------------------------ 561
             +    QF QE                                                
Sbjct: 514 AESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPI 573

Query: 562 -----------------------PEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPS- 612
                                  P P++HRDLKPANILL+ NFVSK+ DVGL+ ++  + 
Sbjct: 574 PWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAAD 633

BLAST of Cp4.1LG06g04710 vs. NCBI nr
Match: KAG7024523.1 (U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1170 bits (3028), Expect = 0.0
Identity = 644/746 (86.33%), Postives = 648/746 (86.86%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWA+DYLLQ+GQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAVDYLLQRGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIV 120

Query: 123 ------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLAQ 182
                       TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLR QLAQ
Sbjct: 121 MGASAKSGFLRHTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRSQLAQ 180

Query: 183 QSSTKSDTPEIQMTHSASAR----------DLPTQESDISFVSSGRPSIDRIFPSLYDSQ 242
           QSSTKSDTPEIQMTHSASAR          DLPTQESDISFVSSGRPSIDRIFPSLYDSQ
Sbjct: 181 QSSTKSDTPEIQMTHSASARGYNYKPSSYGDLPTQESDISFVSSGRPSIDRIFPSLYDSQ 240

Query: 243 DTGGRSTPPQVSSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMED 302
           DTGGRSTPPQVSSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMED
Sbjct: 241 DTGGRSTPPQVSSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMED 300

Query: 303 VEAEMRRLKLELKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALAL 362
           VEAEMRRL+LELKQTMEMYSTACKEALTAKQK VELQRWKLEEEQRLEEARLAGEAALAL
Sbjct: 301 VEAEMRRLRLELKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALAL 360

Query: 363 AEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKY 422
           AEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKY
Sbjct: 361 AEQEKLKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKY 420

Query: 423 TIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE----- 482
           TIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE     
Sbjct: 421 TIDEIEVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLS 480

Query: 483 ------------------------------------------------------------ 542
                                                                       
Sbjct: 481 CIRHPNMVLLLGACPEYGCLVYEYMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALL 540

Query: 543 ------PEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFC 602
                 PEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFC
Sbjct: 541 FLHQTKPEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFC 600

Query: 603 YIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENGTFETILDPAVLD 654
           YIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENGTFETILDPAVLD
Sbjct: 601 YIDPEYQQTGMLGVKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENGTFETILDPAVLD 660

BLAST of Cp4.1LG06g04710 vs. NCBI nr
Match: XP_023536596.1 (U-box domain-containing protein 35-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1170 bits (3026), Expect = 0.0
Identity = 652/787 (82.85%), Postives = 652/787 (82.85%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIV 120

Query: 123 -------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                        TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA
Sbjct: 121 MGASAKSGFLRFKTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR--------------------------------------- 242
           QQSSTKSDTPEIQMTHSASAR                                       
Sbjct: 181 QQSSTKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPCNLPSKVQDETDFMRSPFTR 240

Query: 243 -----------DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDL 302
                      DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDL
Sbjct: 241 KGYNYKPSSYGDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDL 300

Query: 303 PHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMY 362
           PHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMY
Sbjct: 301 PHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMY 360

Query: 363 STACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAAR 422
           STACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAAR
Sbjct: 361 STACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAAR 420

Query: 423 RIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIG 482
           RIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIG
Sbjct: 421 RIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIG 480

Query: 483 EGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE------------------------ 542
           EGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE                        
Sbjct: 481 EGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGC 540

Query: 543 -----------------------------------------------PEPLVHRDLKPAN 602
                                                          PEPLVHRDLKPAN
Sbjct: 541 LVYEYMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPAN 600

Query: 603 ILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 654
           ILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY
Sbjct: 601 ILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 660

BLAST of Cp4.1LG06g04710 vs. NCBI nr
Match: KAG6591640.1 (U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1157 bits (2993), Expect = 0.0
Identity = 645/787 (81.96%), Postives = 649/787 (82.47%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWA+DYLLQ+GQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAVDYLLQRGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIV 120

Query: 123 -------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                        TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLR QLA
Sbjct: 121 MGASAKSGFLRFKTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRSQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR--------------------------------------- 242
           QQSSTKSDTPEIQMTHSASAR                                       
Sbjct: 181 QQSSTKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPCNLPSKVQDDTDFMRSPFTR 240

Query: 243 -----------DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDL 302
                      DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDL
Sbjct: 241 KGYNYKPSSYGDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDL 300

Query: 303 PHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMY 362
           PHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRL+LELKQTMEMY
Sbjct: 301 PHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLRLELKQTMEMY 360

Query: 363 STACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAAR 422
           STACKEALTAKQK VELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAAR
Sbjct: 361 STACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAAR 420

Query: 423 RIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIG 482
           RIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIG
Sbjct: 421 RIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIG 480

Query: 483 EGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE------------------------ 542
           EGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE                        
Sbjct: 481 EGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGC 540

Query: 543 -----------------------------------------------PEPLVHRDLKPAN 602
                                                          PEPLVHRDLKPAN
Sbjct: 541 LVYEYMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPAN 600

Query: 603 ILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 654
           ILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY
Sbjct: 601 ILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 660

BLAST of Cp4.1LG06g04710 vs. NCBI nr
Match: XP_022936885.1 (U-box domain-containing protein 52-like isoform X1 [Cucurbita moschata] >XP_022936886.1 U-box domain-containing protein 52-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 645/795 (81.13%), Postives = 649/795 (81.64%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWA+DYLLQ+GQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAVDYLLQRGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIV 120

Query: 123 -------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                        TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLR QLA
Sbjct: 121 MGASAKSGFLRFKTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRSQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR--------------------------------------- 242
           QQSSTKSDTPEIQMTHSASAR                                       
Sbjct: 181 QQSSTKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPCNLPCNLPCNLPSKVQDDTD 240

Query: 243 -------------------DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQV 302
                              DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQV
Sbjct: 241 FMRSPFTRKGYNYKPSSYGDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQV 300

Query: 303 SSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLE 362
           SSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRL+LE
Sbjct: 301 SSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLRLE 360

Query: 363 LKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAA 422
           LKQTMEMYSTACKEALTAKQK VELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAA
Sbjct: 361 LKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAA 420

Query: 423 IEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEF 482
           IEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEF
Sbjct: 421 IEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEF 480

Query: 483 FAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE---------------- 542
           FAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE                
Sbjct: 481 FAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLL 540

Query: 543 -------------------------------------------------------PEPLV 602
                                                                  PEPLV
Sbjct: 541 GACPEYGCLVYEYMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLV 600

Query: 603 HRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGM 654
           HRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGM
Sbjct: 601 HRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGM 660

BLAST of Cp4.1LG06g04710 vs. NCBI nr
Match: XP_022976246.1 (U-box domain-containing protein 35-like [Cucurbita maxima])

HSP 1 Score: 1121 bits (2900), Expect = 0.0
Identity = 633/781 (81.05%), Postives = 638/781 (81.69%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGL+RKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQ+GQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLDRKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQRGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAA+AIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAARAIIEYAANKAIENIV 120

Query: 123 -------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                        TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA
Sbjct: 121 LGASAKSGFLRFKTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR--------DLP---------------------------- 242
           QQSS KSDTPEIQMTHSASAR        DLP                            
Sbjct: 181 QQSSPKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPCNLPSKVQDETDFMRSPFTR 240

Query: 243 ----------TQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSF 302
                     TQESDISFVSSGR SIDRIFP    SQDTGGR+TPPQVSSSTDFDL HSF
Sbjct: 241 KGYNYKPSSPTQESDISFVSSGRLSIDRIFPC---SQDTGGRATPPQVSSSTDFDLSHSF 300

Query: 303 ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTAC 362
           ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTAC
Sbjct: 301 ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTAC 360

Query: 363 KEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAE 422
           KEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAE
Sbjct: 361 KEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAE 420

Query: 423 LEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGY 482
           LEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGY
Sbjct: 421 LEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGY 480

Query: 483 GPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE---------------------------- 542
           GPVYRC+LDHTPVAIKVLRPDATQGRSQFKQE                            
Sbjct: 481 GPVYRCNLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYE 540

Query: 543 -------------------------------------------PEPLVHRDLKPANILLD 602
                                                      PEPLVHRDLKPANILLD
Sbjct: 541 YMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLD 600

Query: 603 RNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI 652
           RNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI
Sbjct: 601 RNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI 660

BLAST of Cp4.1LG06g04710 vs. ExPASy TrEMBL
Match: A0A6J1FEZ3 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443340 PE=4 SV=1)

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 645/795 (81.13%), Postives = 649/795 (81.64%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWA+DYLLQ+GQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAVDYLLQRGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIV 120

Query: 123 -------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                        TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLR QLA
Sbjct: 121 MGASAKSGFLRFKTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRSQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR--------------------------------------- 242
           QQSSTKSDTPEIQMTHSASAR                                       
Sbjct: 181 QQSSTKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPCNLPCNLPCNLPSKVQDDTD 240

Query: 243 -------------------DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQV 302
                              DLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQV
Sbjct: 241 FMRSPFTRKGYNYKPSSYGDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQV 300

Query: 303 SSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLE 362
           SSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRL+LE
Sbjct: 301 SSSTDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLRLE 360

Query: 363 LKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAA 422
           LKQTMEMYSTACKEALTAKQK VELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAA
Sbjct: 361 LKQTMEMYSTACKEALTAKQKAVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAA 420

Query: 423 IEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEF 482
           IEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEF
Sbjct: 421 IEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEF 480

Query: 483 FAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE---------------- 542
           FAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE                
Sbjct: 481 FAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLL 540

Query: 543 -------------------------------------------------------PEPLV 602
                                                                  PEPLV
Sbjct: 541 GACPEYGCLVYEYMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLV 600

Query: 603 HRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGM 654
           HRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGM
Sbjct: 601 HRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGM 660

BLAST of Cp4.1LG06g04710 vs. ExPASy TrEMBL
Match: A0A6J1IGE2 (U-box domain-containing protein 35-like OS=Cucurbita maxima OX=3661 GN=LOC111476698 PE=4 SV=1)

HSP 1 Score: 1121 bits (2900), Expect = 0.0
Identity = 633/781 (81.05%), Postives = 638/781 (81.69%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRYGL+RKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQ+GQSVFLIHVKLRSPSN
Sbjct: 1   MWLPRYGLDRKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQRGQSVFLIHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI- 122
           SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAA+AIIEYAANKAIENI 
Sbjct: 61  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAARAIIEYAANKAIENIV 120

Query: 123 -------------TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                        TNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA
Sbjct: 121 LGASAKSGFLRFKTNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR--------DLP---------------------------- 242
           QQSS KSDTPEIQMTHSASAR        DLP                            
Sbjct: 181 QQSSPKSDTPEIQMTHSASARASADRPPLDLPCNLPCNLPCNLPSKVQDETDFMRSPFTR 240

Query: 243 ----------TQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSF 302
                     TQESDISFVSSGR SIDRIFP    SQDTGGR+TPPQVSSSTDFDL HSF
Sbjct: 241 KGYNYKPSSPTQESDISFVSSGRLSIDRIFPC---SQDTGGRATPPQVSSSTDFDLSHSF 300

Query: 303 ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTAC 362
           ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTAC
Sbjct: 301 ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTAC 360

Query: 363 KEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAE 422
           KEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAE
Sbjct: 361 KEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAE 420

Query: 423 LEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGY 482
           LEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGY
Sbjct: 421 LEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGY 480

Query: 483 GPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE---------------------------- 542
           GPVYRC+LDHTPVAIKVLRPDATQGRSQFKQE                            
Sbjct: 481 GPVYRCNLDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYE 540

Query: 543 -------------------------------------------PEPLVHRDLKPANILLD 602
                                                      PEPLVHRDLKPANILLD
Sbjct: 541 YMANGSLEDRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLD 600

Query: 603 RNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI 652
           RNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI
Sbjct: 601 RNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGI 660

BLAST of Cp4.1LG06g04710 vs. ExPASy TrEMBL
Match: A0A1S3CQW0 (U-box domain-containing protein 52 OS=Cucumis melo OX=3656 GN=LOC103503739 PE=4 SV=1)

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 572/774 (73.90%), Postives = 604/774 (78.04%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRY LERKEKL+V G VAVAID+DKGSQGALKWAID+LLQ+G +VFLIHVKL+SPSN
Sbjct: 1   MWLPRYSLERKEKLNVNGFVAVAIDKDKGSQGALKWAIDHLLQRGHTVFLIHVKLKSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIT 122
           S+QKM  K D EAS+K+LFLPFRCFCTRKDIQC DVVLEDTD AKAIIEYAAN  IENI 
Sbjct: 61  STQKM--KPDPEASVKDLFLPFRCFCTRKDIQCKDVVLEDTDVAKAIIEYAANTTIENIV 120

Query: 123 --------------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                          DVPGS+VKGAPDFCNVFVISKGK+QSMRSASRPAP +  LRGQLA
Sbjct: 121 VGASAKSGFLRFKATDVPGSIVKGAPDFCNVFVISKGKIQSMRSASRPAPTVGALRGQLA 180

Query: 183 QQSSTKSDTPEIQMTHSASAR-------------------------------------DL 242
           QQ STKSDTP+  ++HSASAR                                     DL
Sbjct: 181 QQCSTKSDTPDFPVSHSASARVSLDKPPLDLPNKSQDEADFMRSPFTRKGYNYKPSYGDL 240

Query: 243 PTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQMGRMS 302
           P Q+SDISFVSSGRPSIDRIFP+LY+SQD  GR+TPPQ+SSSTD DL  SFESLQ+GRMS
Sbjct: 241 PMQDSDISFVSSGRPSIDRIFPTLYESQDMVGRATPPQISSSTDLDLTRSFESLQLGRMS 300

Query: 303 ADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQK 362
            DMNF SEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQK
Sbjct: 301 LDMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQK 360

Query: 363 TVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQKRMKA 422
            VELQRWKLEEEQRLEEARLAGEAALALAEQEK KSKAA+EAAEAARRIAELE QKRMKA
Sbjct: 361 AVELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSKAAVEAAEAARRIAELEAQKRMKA 420

Query: 423 EMKALKEAEEKKKAVDALANND-IRYRKYTIDEIEVATEFFAESRKIGEGGYGPVYRCDL 482
           EMKALKEAEEKKKA DALA  D IRYRKYTI+EIE ATEFF+ESR+IGEGGYGPVY+CDL
Sbjct: 421 EMKALKEAEEKKKAFDALAQTDNIRYRKYTIEEIEAATEFFSESRRIGEGGYGPVYKCDL 480

Query: 483 DHTPVAIKVLRPDATQGRSQFKQE------------------------------------ 542
           DHTPVAIKVLRPDATQGRSQFKQE                                    
Sbjct: 481 DHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLE 540

Query: 543 -----------------------------------PEPLVHRDLKPANILLDRNFVSKIS 602
                                              PEPLVHRDLKPANILLDRNFVSKIS
Sbjct: 541 DRLLRRGNTPPLSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKIS 600

Query: 603 DVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITS 650
           DVGLARLVPPSIA+SVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITS
Sbjct: 601 DVGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITS 660

BLAST of Cp4.1LG06g04710 vs. ExPASy TrEMBL
Match: A0A0A0LHG8 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G882930 PE=4 SV=1)

HSP 1 Score: 1005 bits (2598), Expect = 0.0
Identity = 564/775 (72.77%), Postives = 603/775 (77.81%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRY LERKE+L+V GIVAVAID+DKGSQGALKWAID+LLQ+G +VFL+HVKL+SPSN
Sbjct: 1   MWLPRYSLERKERLNVNGIVAVAIDKDKGSQGALKWAIDHLLQRGHTVFLMHVKLKSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIT 122
           S+QKM  KSD EA++K+LFLPFRCFCTRKDIQC D+VLEDTD AKAIIEYAAN  IENI 
Sbjct: 61  STQKM--KSDPEANVKDLFLPFRCFCTRKDIQCKDIVLEDTDVAKAIIEYAANTTIENIV 120

Query: 123 --------------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                          D+PGS+VKGAPDFCNVFVISKGK+Q+MRSASRPAP +S LRG LA
Sbjct: 121 VGASARSGFLRFKVTDIPGSIVKGAPDFCNVFVISKGKIQTMRSASRPAPTVSALRGPLA 180

Query: 183 QQSSTK-SDTPEIQMTHSASAR-------------------------------------D 242
           QQSS K SDTP+  ++HSA+AR                                     D
Sbjct: 181 QQSSKKLSDTPDFPVSHSATARVSLDKPPLDLPNKSQDEADYMRSPFTRKGYNYKPSYGD 240

Query: 243 LPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQMGRM 302
           LP Q+SDISFVSSGRPSIDRIFP+LY+SQD GGR+TPPQ+SSSTD DL  SFESLQ+GRM
Sbjct: 241 LPMQDSDISFVSSGRPSIDRIFPTLYESQDMGGRATPPQISSSTDLDLTRSFESLQLGRM 300

Query: 303 SADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQ 362
           S DMNF SEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQ
Sbjct: 301 SVDMNFPSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQ 360

Query: 363 KTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQKRMK 422
           K VELQRWKLEEEQRLEEARLAGEAALALAEQEK KSKAA+EAAEAARRIAELE QKRMK
Sbjct: 361 KAVELQRWKLEEEQRLEEARLAGEAALALAEQEKAKSKAAVEAAEAARRIAELEAQKRMK 420

Query: 423 AEMKALKEAEEKKKAVDALANND-IRYRKYTIDEIEVATEFFAESRKIGEGGYGPVYRCD 482
           AEMKALKEAEEKKKA DALA  D IRYRKYTI+EIE ATEFF+ESR+IGEGGYGPVY+CD
Sbjct: 421 AEMKALKEAEEKKKAYDALAQTDNIRYRKYTIEEIEAATEFFSESRRIGEGGYGPVYKCD 480

Query: 483 LDHTPVAIKVLRPDATQGRSQFKQE----------------------------------- 542
           LDHTPVAIKVLRPDATQGRSQFKQE                                   
Sbjct: 481 LDHTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSL 540

Query: 543 ------------------------------------PEPLVHRDLKPANILLDRNFVSKI 602
                                               PEPLVHRDLKPANILLDRNFVSKI
Sbjct: 541 EDRLLRRGNTPALSWQLRFKIAAEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKI 600

Query: 603 SDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT 650
           SDVGLARLVPPSIA+SVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT
Sbjct: 601 SDVGLARLVPPSIADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT 660

BLAST of Cp4.1LG06g04710 vs. ExPASy TrEMBL
Match: A0A6J1FML4 (U-box domain-containing protein 52-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446783 PE=4 SV=1)

HSP 1 Score: 990 bits (2559), Expect = 0.0
Identity = 558/774 (72.09%), Postives = 596/774 (77.00%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLPRY L+RKE+L+V G VAVAID+DKGSQGALKWAID+LL++GQ+VFL+HVKLRSPSN
Sbjct: 1   MWLPRYSLDRKERLNVNGFVAVAIDKDKGSQGALKWAIDHLLRRGQTVFLVHVKLRSPSN 60

Query: 63  SSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENIT 122
           SS KM  KSD EA ++ELFLPFRCFCTRKDIQC DVVLEDTD AKA+ EYAAN AIENI 
Sbjct: 61  SSLKM--KSDPEAGVRELFLPFRCFCTRKDIQCKDVVLEDTDIAKALTEYAANTAIENIV 120

Query: 123 --------------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRGQLA 182
                          DVPG+V KGAPDFCNVFVISKGK+QSMRSASRPAPV++PLR QL 
Sbjct: 121 VGASSKSGFLRFKATDVPGNVSKGAPDFCNVFVISKGKIQSMRSASRPAPVVNPLRSQLV 180

Query: 183 QQSSTKSDTPEIQMTHSASAR-------------------------------------DL 242
           QQSSTKS + + Q   + SAR                                     D 
Sbjct: 181 QQSSTKSISSDYQFPQTTSARASLDKRPFVLPRRSQDEADFMRSPFTRKGYNYKPSYGDS 240

Query: 243 PTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQMGRMS 302
             QESDIS+VSSGRPSIDRIFPS+YDS D GGR+TPP++SSSTD DL  SFESL MGR+S
Sbjct: 241 SFQESDISYVSSGRPSIDRIFPSVYDSLDMGGRATPPRMSSSTDLDLTQSFESLPMGRLS 300

Query: 303 ADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQK 362
            DMNF SEFSSISQDSDRLSISSQS+EDVEAEMRRLKLELKQTMEMYSTACKEALTAKQK
Sbjct: 301 MDMNFPSEFSSISQDSDRLSISSQSLEDVEAEMRRLKLELKQTMEMYSTACKEALTAKQK 360

Query: 363 TVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQKRMKA 422
            VELQRWKLEEEQR+EEARLAGEAALALAEQEK KSKAAIEAAEAARRIAELE QKRMKA
Sbjct: 361 AVELQRWKLEEEQRIEEARLAGEAALALAEQEKAKSKAAIEAAEAARRIAELEAQKRMKA 420

Query: 423 EMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPVYRCDLD 482
           EMKA KEAEEKK+A+DAL+ NDIRYRKYTI+EIE ATEFFAESRKIGEGGYGPVY+CDLD
Sbjct: 421 EMKAHKEAEEKKRAIDALSQNDIRYRKYTIEEIEEATEFFAESRKIGEGGYGPVYKCDLD 480

Query: 483 HTPVAIKVLRPDATQGRSQFKQE------------------------------------- 542
           HTPVAIKVLRPDATQGRSQFKQE                                     
Sbjct: 481 HTPVAIKVLRPDATQGRSQFKQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLED 540

Query: 543 ----------------------------------PEPLVHRDLKPANILLDRNFVSKISD 602
                                             PEPLVHRDLKPANILLDRNFVSKISD
Sbjct: 541 RLLQRGNTPPLSWQLRFQIAGEIGTALLFLHQTKPEPLVHRDLKPANILLDRNFVSKISD 600

Query: 603 VGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSR 651
           VGLARLVPPS+A++VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSR
Sbjct: 601 VGLARLVPPSVADNVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITSR 660

BLAST of Cp4.1LG06g04710 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 747.7 bits (1929), Expect = 8.1e-216
Identity = 435/782 (55.63%), Postives = 519/782 (66.37%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLR-SPS 62
           MW+P+      ++    G+VAVAID+DK SQ ALKWA+D LLQ+GQSV L+HVKLR SP 
Sbjct: 1   MWMPKNNNVGVKEGGSSGVVAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRPSPL 60

Query: 63  N-------SSQKMSSKS-----DSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAI 122
           N       SS K+S  S     D E   KE+FLPFRCFCTRKDIQC DV+LE++D AKA+
Sbjct: 61  NNSASLHASSAKLSQDSSLVCRDPEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKAL 120

Query: 123 IEYAANKAIE---------------NITNDVPGSVVKGAPDFCNVFVISKGKVQSMRSAS 182
           +EY    AIE               N   D+PGS+ K APDFC V++ISKGK+Q+MRSAS
Sbjct: 121 VEYVNQAAIEVLVVGSSSKGGFLRFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSAS 180

Query: 183 RPAPVISPLRGQLA-----------------------------------QQSSTKSD--- 242
           R AP+ +PLR  +                                    QQ  +  D   
Sbjct: 181 RSAPMTAPLRSSVQPPSLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQR 240

Query: 243 -----------TPEIQMTHSASARDLPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRS 302
                      +P  +  +  S  DL   ESDISF+SSGRPSIDRIFPSLYD+ D     
Sbjct: 241 RSIDDQSESFRSPFTRRGNGRSYGDLTVPESDISFISSGRPSIDRIFPSLYDNNDPS--R 300

Query: 303 TPPQVSSSTDFDLPHSF-ESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEM 362
           TPP++S+ +D D   S  +S   GR S DMN  ++F     +S+R S +SQS++DVEAEM
Sbjct: 301 TPPRLSNFSDMDYSSSLDQSSNYGRRSVDMNSPTDF-----ESERFSSASQSIDDVEAEM 360

Query: 363 RRLKLELKQTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEK 422
           RRLKLELKQTMEMYSTACKEALTAKQK  ELQRWKLEEE++LEEAR A EAALA+AE+EK
Sbjct: 361 RRLKLELKQTMEMYSTACKEALTAKQKATELQRWKLEEERKLEEARNAEEAALAIAEKEK 420

Query: 423 LKSKAAIEAAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEI 482
            KSKAA+EAAEAA+RIAELE +KR+ AEMKALKE+EEK KA+ ALAN+D+RYRKY+I++I
Sbjct: 421 AKSKAAMEAAEAAQRIAELEAKKRVNAEMKALKESEEKTKALTALANSDVRYRKYSIEDI 480

Query: 483 EVATEFFAESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE---------- 542
           E+ATEFFAE  KIGEGGYGPVY+C LDHTPVA+KVLRPDA QGRSQF+QE          
Sbjct: 481 ELATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSCIRHP 540

Query: 543 ------------------------------------------------------------ 602
                                                                       
Sbjct: 541 NMVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLFLHQA 600

Query: 603 -PEPLVHRDLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPE 636
            PEPLVHRDLKP NILLDRNFVSKISDVGLARLVPP++A++VTQYRMTSTAGTFCYIDPE
Sbjct: 601 KPEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCYIDPE 660

BLAST of Cp4.1LG06g04710 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 693.0 bits (1787), Expect = 2.4e-199
Identity = 405/758 (53.43%), Postives = 497/758 (65.57%), Query Frame = 0

Query: 11  ERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSP---------S 70
           +R     V G+VAVAIDRDK SQGALKWA+D LLQKGQ+V L+HVK R+          S
Sbjct: 7   DRDGGFGVDGLVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINS 66

Query: 71  NSSQKMSSKSDS-------EASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAA 130
           NSS+      DS       E S K+LFLPFRC C+RKDIQC DV+LE++D A+A++EYA 
Sbjct: 67  NSSKTSQINGDSSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYAN 126

Query: 131 NKAIE---------------NITNDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPV 190
              IE               N   D+PG++ K APDFC V+ I+KGK+ + ++ASR AP 
Sbjct: 127 QVMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPS 186

Query: 191 ISPLRGQLAQQ------------SSTKSDTPEIQMTHSASARD----------------- 250
           +SPLR QL Q             ++T+++    +  H  S  D                 
Sbjct: 187 VSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFESQHRRSLDDQSDSFRPPYNKRGLTGR 246

Query: 251 ----LPTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQ 310
               L   +S+ISF SSGRPSI+R  PSLYD+ D     TPP++S+ +D D   SFES+ 
Sbjct: 247 SYGELSIPDSEISFNSSGRPSIERNSPSLYDNSDP--NRTPPRLSNFSDVDY-CSFESMT 306

Query: 311 MGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEAL 370
            GR S D++  + FS+ S +++R S +SQ  +DVEAEMRRLKLELKQTMEMYSTACKEAL
Sbjct: 307 FGRRSMDLSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKEAL 366

Query: 371 TAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQ 430
           TAK K  ELQRWKL EE++ EEA+LA EAALA+AE+EK KSKAA+EAAEAA+RIA++E +
Sbjct: 367 TAKHKATELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAMEAAEAAQRIADIESR 426

Query: 431 KRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPVY 490
           KR+ AE KALKE+E + KAV+ALA  D+RYRKY+I+EIE ATEFF +  KIGEG YGPVY
Sbjct: 427 KRVDAETKALKESEARTKAVNALA-KDVRYRKYSIEEIEDATEFFDDKYKIGEGSYGPVY 486

Query: 491 RCDLDHTPVAIKVLRPDATQGRSQFKQE-------------------------------- 550
           +C LDHTPVA+K LRPDA QGRSQF++E                                
Sbjct: 487 KCYLDHTPVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFMAN 546

Query: 551 ---------------------------------------PEPLVHRDLKPANILLDRNFV 610
                                                  PEPLVHRDLKPANILLDRNFV
Sbjct: 547 GSLEDRLFRQGDSPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRNFV 606

Query: 611 SKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQ 634
           SK++DVGLARLVPPS+AN+VTQY MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQ
Sbjct: 607 SKLADVGLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQ 666

BLAST of Cp4.1LG06g04710 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 591.7 bits (1524), Expect = 7.4e-169
Identity = 361/764 (47.25%), Postives = 473/764 (61.91%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSN 62
           MWLP+    +KE     G VAVAID+DKGSQ ALKW ID L  +GQ++ LIHV  RS S+
Sbjct: 1   MWLPKANGAKKE--TGSGSVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCRSHSS 60

Query: 63  SSQKMSS---KSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIE 122
           S  +  +   K   E   K+LF+ F C+C+RK+I C D++LED D  +AI EY ++ AIE
Sbjct: 61  SDLEEGTPQQKQQMEKIAKDLFVSFHCYCSRKEINCRDILLEDADKVRAITEYVSSSAIE 120

Query: 123 NIT--------------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAPVISPLRG 182
           N+                D+P +V K APDFCNV+VISKGK+ S+R+ASRPAP  + ++ 
Sbjct: 121 NLVVGSASRNGFMRRFKTDLPTTVSKSAPDFCNVYVISKGKIASVRNASRPAPYQNSMQQ 180

Query: 183 -QLAQQSSTKSDTPEIQMTHSASARDLPT------------------------------- 242
            ++        D       H  SA   P+                               
Sbjct: 181 CEIDNHHPHTPDKAPKYHDHPNSAGSTPSRPRKSVEADRSPLVKRKPYGDFYDSDSDLSF 240

Query: 243 ------QESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQM 302
                 ++SDISF+SSGRPS++R   SL    D    +   ++S+S++     S  S ++
Sbjct: 241 ISPSSHRDSDISFISSGRPSVERSSFSL----DFPESARTSRMSTSSE----QSIGSNRL 300

Query: 303 GRMSADMNFSSEFSSISQDSDRL-SISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEAL 362
           G   +D +F +E S+ S++S R  S SSQS++DVEAEM+RL+LELKQTM+MYSTACKEAL
Sbjct: 301 GIKFSDPDFLNESSTFSEESGRTSSYSSQSLDDVEAEMKRLRLELKQTMDMYSTACKEAL 360

Query: 363 TAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEGQ 422
           +A+Q+  ELQ+ + EEE+RLEEA+ + EAA+++ E+E+ K+KAA+EAAEAA+R+AE+E +
Sbjct: 361 SARQQATELQKLRTEEERRLEEAKSSEEAAMSIVEKERAKAKAALEAAEAAKRLAEVESK 420

Query: 423 KRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPVY 482
           +R+ AEMK +KE+       D+ +   +RYRKYT+DEIE AT  FAES+K+GEGGYGPV+
Sbjct: 421 RRLTAEMKTMKES-------DSFSRGFVRYRKYTVDEIEEATSNFAESQKVGEGGYGPVF 480

Query: 483 RCDLDHTPVAIKVLRPDATQGRSQFKQE-------------------------------- 542
           R  LDHT VA+KVLRPDA QGRSQF++E                                
Sbjct: 481 RGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLSCIRHPNMVLLLGACPEFGILVYEYMAK 540

Query: 543 ---------------------------------------PEPLVHRDLKPANILLDRNFV 602
                                                  PEP+VHRDLKP N+LLD N+V
Sbjct: 541 GSLEDRLFMRGNTPPITWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDYNYV 600

Query: 603 SKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQ 640
           SKISDVGLARLV P++A +VTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLGIM LQ
Sbjct: 601 SKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIMLLQ 660

BLAST of Cp4.1LG06g04710 vs. TAIR 10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 572.0 bits (1473), Expect = 6.1e-163
Identity = 351/733 (47.89%), Postives = 454/733 (61.94%), Query Frame = 0

Query: 22  VAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHVKLRSPSNSSQKMSSKSDSEASIKELF 81
           + +AIDRDK SQ ALKWA+  LL +GQ++ L+HVKL+ PS+     S+ S       ELF
Sbjct: 12  ITLAIDRDKESQNALKWAVSNLLSRGQTLTLLHVKLKQPSSLPYSGSNFSKPGDDPSELF 71

Query: 82  LPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIENI-------------TNDVPGS 141
           LPFRC+C RKDI C DVV+ED  AAK I++Y    AIE +               DV  +
Sbjct: 72  LPFRCYCARKDINCQDVVVEDVSAAKGIVDYVQQNAIETLILGSSKMTLLRFKAADVSST 131

Query: 142 VVKGAPDFCNVFVISKGKVQSMRSASRPAP---VISPLRGQLAQQSSTKSDTPE--IQMT 201
           V+K AP FC V+VISKGK+  +RSA+   P   + S      AQ S+   +  +  +Q T
Sbjct: 132 VMKKAPSFCTVYVISKGKISFLRSATSSPPHSNMPSMRHHSHAQTSNMNVERRQQTMQRT 191

Query: 202 HSASARDL----------PTQESDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSS 261
           H     ++             +SDISFVSSGRPS+D++FPSLYD  D    S   +   +
Sbjct: 192 HDEIKIEIKRGYEGVYQPSITDSDISFVSSGRPSVDQMFPSLYDDVDVPRLSVTSEYGEN 251

Query: 262 -TDFDLPHSFESLQMGRMSADMNFSSEFSSISQDSDRLSISSQSMEDVEAEMRRLKLELK 321
              F   +S +S+ +G   A        SS S +S R S S Q  +++E EMRRLK+ELK
Sbjct: 252 RLSFATTYSKQSIDLGSPYAPN------SSTSFESGRQSFSLQGQDELETEMRRLKMELK 311

Query: 322 QTMEMYSTACKEALTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIE 381
            TMEMY++ACKEA++AK+   EL +WK E+E +LEE RL+ EAA+A+AE+EK KS+AA+E
Sbjct: 312 HTMEMYNSACKEAISAKKAANELLKWKAEKEHKLEEVRLSKEAAMAMAEREKEKSRAAME 371

Query: 382 AAEAARRIAELEGQKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFA 441
           AA AA+++++LE +KR     K ++  +EKK+AV +L     RYRKYTI+EIE ATE F+
Sbjct: 372 AAVAAQKLSDLEAEKR-----KHIETVDEKKRAVSSL-----RYRKYTIEEIEEATEDFS 431

Query: 442 ESRKIGEGGYGPVYRCDLDHTPVAIKVLRPDATQGRSQFKQE------------------ 501
            SRK+GEGGYGPVY+  LD+T VAIKVLRPDA QGRSQF++E                  
Sbjct: 432 PSRKVGEGGYGPVYKGTLDYTKVAIKVLRPDAAQGRSQFQREVEVLTCMRHPNMVLLLGA 491

Query: 502 -----------------------------------------------------PEPLVHR 561
                                                                PEPLVHR
Sbjct: 492 CPEYGCLVYEYMANGSLDDCLFRRGNSPILSWQLRFRIASEIATGLHFLHQMKPEPLVHR 551

Query: 562 DLKPANILLDRNFVSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLG 621
           DLKP NILLD++FVSKISDVGLARLVPPS+A++ TQYRMTSTAGTF YIDPEYQQTGMLG
Sbjct: 552 DLKPGNILLDQHFVSKISDVGLARLVPPSVADTATQYRMTSTAGTFFYIDPEYQQTGMLG 611

Query: 622 VKSDIYSLGIMFLQLITSRPPMGLTHHVERAIENGTFETILDPAVLDWPIEEALSFAKMS 647
            KSDIYS GIM LQ++T++PPMGLTHHVE+AIE GTF  +LDPAV DWP EEAL+ AK++
Sbjct: 612 TKSDIYSFGIMLLQILTAKPPMGLTHHVEKAIEKGTFAEMLDPAVPDWPFEEALAAAKLA 671

BLAST of Cp4.1LG06g04710 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 561.2 bits (1445), Expect = 1.1e-159
Identity = 348/759 (45.85%), Postives = 463/759 (61.00%), Query Frame = 0

Query: 3   MWLPRYGLERKEKLDVKGIVAVAIDRDKGSQGALKWAIDYLLQKGQSVFLIHV--KLRSP 62
           MWLP+    +K   +  G VA+AID+DK SQ A+KW ++ L  +GQ++ LIHV  K +S 
Sbjct: 1   MWLPKADATKKGTRN--GSVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHVLPKSQSS 60

Query: 63  SNSSQKMSSKSDSEASIKELFLPFRCFCTRKDIQCMDVVLEDTDAAKAIIEYAANKAIEN 122
            +  + ++ K   E   K+LF+ F C+C+RK+I C+DVVLED D  KAI+EY    AIEN
Sbjct: 61  LDIEEGITHKQQMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIEN 120

Query: 123 IT--------------NDVPGSVVKGAPDFCNVFVISKGKVQSMRSASRPAP----VISP 182
           +                D+P SV K APDFCNV+VISKGK+ S+R+ASR AP    V+S 
Sbjct: 121 LVLGAPSRNSFMRRFKTDLPTSVSKAAPDFCNVYVISKGKISSLRNASRLAPYHPSVLSE 180

Query: 183 L---------------------RGQLAQQSS-TKSDTPEIQMTHSASARDLPTQE----- 242
           +                     +G+ +  S  T+   P+    H     D    E     
Sbjct: 181 VDDHETIAIERKHKTANTPALPKGRRSIDSDVTRLGLPKPPHGHMKLMGDFSDSESEFSF 240

Query: 243 --------SDISFVSSGRPSIDRIFPSLYDSQDTGGRSTPPQVSSSTDFDLPHSFESLQM 302
                   SD+SF+SSGRPS+DR     YD  ++   S   ++S+S++     S  S ++
Sbjct: 241 ISASQQESSDLSFISSGRPSVDR-SSFTYDLPESARTS---RMSTSSE----QSIGSHRL 300

Query: 303 GRMSADMNFSSEFSSISQDSDR--LSISSQSMEDVEAEMRRLKLELKQTMEMYSTACKEA 362
           G    D+++ +  SS+S +S R   S SSQS+ DVEA+M+RL+LELKQTM+MYS+AC+EA
Sbjct: 301 GIKFTDLSYLNGSSSVSDESGRTSCSFSSQSLNDVEAQMKRLRLELKQTMDMYSSACREA 360

Query: 363 LTAKQKTVELQRWKLEEEQRLEEARLAGEAALALAEQEKLKSKAAIEAAEAARRIAELEG 422
           LTA+ +  ELQ+ + EEE+RLEE ++  E A+++ E E+ K+K A+EAAEAA R+AE+E 
Sbjct: 361 LTARNEATELQKLRTEEERRLEELKMTEETAMSIVENERAKAKTALEAAEAANRLAEVEA 420

Query: 423 QKRMKAEMKALKEAEEKKKAVDALANNDIRYRKYTIDEIEVATEFFAESRKIGEGGYGPV 482
           ++R+ AEMK LKE++   +      ++ +RYRKY++ EIE  T  FAESRK+GEGGYGPV
Sbjct: 421 KRRVHAEMKVLKESDSFSR------HSIVRYRKYSVQEIEEGTANFAESRKVGEGGYGPV 480

Query: 483 YRCDLDHTPVAIKVLRPDATQGRSQFKQE------------------------------- 542
           +R  LDHT VA+KVLRPDA QGRSQF +E                               
Sbjct: 481 FRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLSCIRHPNMVLLLGACPEYGILVYEYMA 540

Query: 543 ----------------------------------------PEPLVHRDLKPANILLDRNF 602
                                                   PEP+VHRDLKP N+LLD N+
Sbjct: 541 RGSLDDRLFRRGNTPPISWQLRFRIAAEIATGLLFLHQTKPEPIVHRDLKPGNVLLDHNY 600

Query: 603 VSKISDVGLARLVPPSIANSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFL 634
           VSKISDVGLARLV P++A +VTQYR+TS AGTFCYIDPEYQQTGMLGVKSD+YSLGIM L
Sbjct: 601 VSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLGVKSDVYSLGIMLL 660