Cp4.1LG06g04070 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG06g04070
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Rhamnogalacturonan endolyase
Location	Cp4.1LG06: 2689080 .. 2692870 (-)
RNA-Seq Expression	Cp4.1LG06g04070
Synteny	Cp4.1LG06g04070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATAGCAGATAACAGAAAGAGATGGATGCCACTCCCTGAGGATAGAGTAGGCCGCAGAGGCAAAACCTTGGATTTTCCTGAAGCTGTATTACTAGTCCATCCTAGAGAAGCTGCGTTCAAGGGAGAGGTGGACGATAAGTACCAATATTCTTGTGAAAATAAGGATCTTAAAGTGCATGGATGGATATCCATGGATCCTTCCGACGACCCTCCGGTGGGTTTCTGGCAAATCACTCCGAGCAGCGAGTTCCGATCCGGCGGACCGCTCAAACAGAACCTCACCTCTCATGTGGGACCATATGCTCTTGCGGTAAGTATTACCTCCTGGATATTGTCTACTTTGAGCCTAAGCTCTCGTCACTGAGGTGGGACTCCTCTCCCAACAATCCTCAATAATACTCCCCTCGAACAAAATACGCCATAGAGCCTCCCATGAGGCCTATGAAACCCTCGAACAACTTTCCCTTAATCGAGGTTCGACTATTTCTTTGTAGCCCTCGAACACTTTAGTCTTTTGACTACAATTTTGAGGCTTACAACTTCTTTGTTCAATATTTGATAATTCTATGAGGCTTATGGAACCCTCGAACAACCTCCCCTTAATCGAGGTTCGACTTTTTCTTTAGAACCCTCGAACACTTTAGTCTTTTGACTACAACTTCGAGACTCACAACTTCTTTGTTCGATATCTGATAATTTTATTGACATCACGTATTCCTTATAAATTTATGATCAACCCCTTAATCAGCGGACGCGAACCAAGTTCAGTATTAAAGAATTTACATAATTGGCACAAATTTCAGATGTTTCTTAGCGCTCACTATGGAGGAGAGGAGTTGGTGCTGAAGCTGAATCCTGATGAGCCGTGGAAGAAAGTGTTTGGGCCTGTCTTTTTCTATCTTAATTCTGTTCCTAATGGAACTGATCCACTTTCTTTGTGGGAAGATGCTAAAATTCAGGTATTTTTCATTTATTTATTTATTTTTATTATGAATATATATATATTTATTTTAATCTCACTAATTTCTCAGATGACGAAGGAAGTTCAAAGCTGGCCCTATTCTTTTCCAGCTTCTGAGAATTTCCCACCACCAAACCAACGTGGTAGATTGACTGGCAACTTATTTGTCCTTGATAGGTACGTAACAACTCAACTCCACCGCTAGTAGATATTGTCCGTTTTCGTCGTTACGTATGGCTGTCAGCCTCCTTTGGAATCTAGCAAGGAATCTAACAATACCCTCGTGGGGTCCAACGTCATAAATGACACACCGCTCGGTTTCTGGCACTGATACCATTTGTAACAACTTAAGCTCACCGCTAATCAATATTGTCCATTTTGGCTCATTATGTATTGTGTTGTGAGATTCCTCGCTGGACCCTAAAAGGGGTGGATTGTGAGATCTGGCATTGGTTGGAGAGGAGAACAAAATATTTCTGATAAGGGTACTGTGAGACTTACGACGATATATAACGAGCTAAATTGGACAATATCTGGTAGGGGTGAGCTTGGATTATTACAAATGGTATTAGAGTCAGACACCGAATGATGTCCTTCCTTATAAAAGTGTGGAAACTTCCTCGTAACAGACGTGTTTTTAAACTTTGAAGCGGATGGCGATAAATTATTTTTATAAAAATTAATAGTAATTTCACTGCATTTGTGAGCAGATATGCTAAGCAAGAGCAAATGCCGGCAGCTGAGGCATATGTGGGACTGGCAGCGCCGGGAGACGCTGGATCATGGCAGACCGAAACCAAGGTACTGAGCGATTGATTATTTGATTTACACCAAATAATGAGAACTAAGAAAATGATGTTATTTAAACCATGATCAGGGCTACCAATTTTGGACCAAAACAGATGCATATGGCTCGTTTAGCATCGAAAATGTACGCATCGGCGACTACAATCTTTATGCTTGGGTTCCTGGCTTCATTGGTGACTACCGTTACGACGTTGTCATTAGCGTCTCTGCAGGTTTTGATTCACATTTCAAATATCTTCCTTTAATTAAGAATAAAATATACATTATGAATTAAGAGGTTAGACGTTCAAATTCACAAAAGACAAAATTCTACGTGAATGAAGCTTATAACTCGAGAAGTGAAAGAACTCTCAACATTTGTAATTATATACAGGTAGTGATATGGACGTGGGGGATTTTGTTTTTGAGCCGCCTAGGAATGGTCCAACGCTATGGGAAATAGGCATTCCTGATCGCTCTGCAGCTGAATTCTATATTCCTGATCCTAACCCAAATTACATTAACAAATTATACGTCGACCATCCAGATAGGTACGAGAGCTTTTCTCTTTCAACTACAGTTAATCGCTTTTCTCTTTCGATTTGGGACTAGTCTCCATAGAGTCTTAGTCCCCCGAATTAAATGAAAATCTCTGACATATATCATTAATTTGACAACTACGCTCAAAGTAATATGTCATTAATTATTTTATTTTATTTTAAATTAAATAGCTTAAATTCTTAGAATATTACTGTTGAGCAGATTCAGGCAATATGGACTGTGGGAGAGATATGCAGAATTATATCCTGATACAGACTTAGTATATACCGTTGGAGTTAGTGATTACACAAAAGATTGGTTCTTCGCTCATGTTAATCGGTAAGTTCCAATATAATGCTAATATGTCAATTAAAATATACCAAATTTATTAGGTTTTTAATTAATTATATTTAAATTAATTGCAGGACCAGAAATGATGGGTCCCATTTACCAACCACGTGGCAAATCAAGTTTACACTCGATAACAACATAGATAAGAGCGGTATCTATAAATTGCGAGTGGCTTTGGCCACCGCCAATGTTGCTGAATTACAGGTACTCATTCTATTATATTGTTAACTTTATGAGACATTTGGTGATATAAGATTTTAAGCATTTTAGATCCAACGATTAGCTGTGTTGGATGATGAAAGTCTTGCATCGGCTCAATTAGGGGATGATTATAAGTTTATAATTAAGGAACACTCTCTCCATTAGTATTAGGCTTTTTGGGGAAGTCCAAAGTAAAGCCATGAGAGCTTATGCTCAANGATGATTATAAGTTTATAATTAAGGAACACTCTCTCCATTAGTATGAGGCCTTTTGGGGAAGTCCAAAGTAAAGCCATGAGAGCTTATGCTCAAAGTGGGCAATATCATACCATTGTGGAGGGTCGTGTTCGTCTAACATGGTATCAGAGCCTTGCCCTAAACTTAGTCGTGCCAATAAAATGATAAATAATTTAGGGAACGATCATGGTTTTTTTTTTTTTTTTTTTTTTTTTNTCATCATTTACTTACGATGTGATATTAATTTTTCTTTTCAAATAAAAATAGATTCGGGTGAACAATCCAGAAGTAGAGTCGGCTCTATTTACGACAGGACAAATTGGGCACGATAATGCAATTGCAAGACATGGAATTCATGGACTCTATCGACTCTATAGTGTCGACATATCGGGATCTGTACTGGTGGAAGGAGAGAACACCATCTTGCTTTCACAAATTATAAACAATTCATCTTTCAACGGCGTCATGTACGATTATATCCGCCTCGAAGGCCCCCCATCCGACGTCTCTACACATCCATAAAATATATGTTTTTTTCTTTTTTTATTTCAAAATTTATAATTATAGTCTTAGTCTTGTAACTCTATGAAACTTGAGCCAATAATGTTGTCAACTAAAAGTTTGTCAGAGATTTAATTACCGAGTCAATTTTTAACCGTTTAGAATAAATTTTAACGGTGTCAATGTGAAAATATAATTTTTCACCATTA

mRNA sequence

ATGGCGATAGCAGATAACAGAAAGAGATGGATGCCACTCCCTGAGGATAGAGTAGGCCGCAGAGGCAAAACCTTGGATTTTCCTGAAGCTGTATTACTAGTCCATCCTAGAGAAGCTGCGTTCAAGGGAGAGGTGGACGATAAGTACCAATATTCTTGTGAAAATAAGGATCTTAAAGTGCATGGATGGATATCCATGGATCCTTCCGACGACCCTCCGATGTTTCTTAGCGCTCACTATGGAGGAGAGGAGTTGGTGCTGAAGCTGAATCCTGATGAGCCGTGGAAGAAAGTGTTTGGGCCTGTCTTTTTCTATCTTAATTCTGTTCCTAATGGAACTGATCCACTTTCTTTGTGGGAAGATGCTAAAATTCAGCAAGAGCAAATGCCGGCAGCTGAGGCATATGTGGGACTGGCAGCGCCGGGAGACGCTGGATCATGGCAGACCGAAACCAAGGGCTACCAATTTTGGACCAAAACAGATGCATATGGCTCGTTTAGCATCGAAAATGTACGCATCGGCGACTACAATCTTTATGCTTGGGTTCCTGGCTTCATTGGTGACTACCGTTACGACGTTGTCATTAGCGTCTCTGCAGGTAGTGATATGGACGTGGGGGATTTTGTTTTTGAGCCGCCTAGGAATGGTCCAACGCTATGGGAAATAGGCATTCCTGATCGCTCTGCAGCTGAATTCTATATTCCTGATCCTAACCCAAATTACATTAACAAATTATACGTCGACCATCCAGATAGATTCAGGCAATATGGACTGTGGGAGAGATATGCAGAATTATATCCTGATACAGACTTAGTATATACCGTTGGAGTTAGTGATTACACAAAAGATTGGTTCTTCGCTCATGTTAATCGGACCAGAAATGATGGGTCCCATTTACCAACCACGTGGCAAATCAAGTTTACACTCGATAACAACATAGATAAGAGCGGTATCTATAAATTGCGAGTGGCTTTGGCCACCGCCAATGTTGCTGAATTACAGATTCGGGTGAACAATCCAGAAGTAGAGTCGGCTCTATTTACGACAGGACAAATTGGGCACGATAATGCAATTGCAAGACATGGAATTCATGGACTCTATCGACTCTATAGTGTCGACATATCGGGATCTGTACTGGTGGAAGGAGAGAACACCATCTTGCTTTCACAAATTATAAACAATTCATCTTTCAACGGCGTCATGTACGATTATATCCGCCTCGAAGGCCCCCCATCCGACGTCTCTACACATCCATAAAATATATGTTTTTTTCTTTTTTTATTTCAAAATTTATAATTATAGTCTTAGTCTTGTAACTCTATGAAACTTGAGCCAATAATGTTGTCAACTAAAAGTTTGTCAGAGATTTAATTACCGAGTCAATTTTTAACCGTTTAGAATAAATTTTAACGGTGTCAATGTGAAAATATAATTTTTCACCATTA

Coding sequence (CDS)

ATGGCGATAGCAGATAACAGAAAGAGATGGATGCCACTCCCTGAGGATAGAGTAGGCCGCAGAGGCAAAACCTTGGATTTTCCTGAAGCTGTATTACTAGTCCATCCTAGAGAAGCTGCGTTCAAGGGAGAGGTGGACGATAAGTACCAATATTCTTGTGAAAATAAGGATCTTAAAGTGCATGGATGGATATCCATGGATCCTTCCGACGACCCTCCGATGTTTCTTAGCGCTCACTATGGAGGAGAGGAGTTGGTGCTGAAGCTGAATCCTGATGAGCCGTGGAAGAAAGTGTTTGGGCCTGTCTTTTTCTATCTTAATTCTGTTCCTAATGGAACTGATCCACTTTCTTTGTGGGAAGATGCTAAAATTCAGCAAGAGCAAATGCCGGCAGCTGAGGCATATGTGGGACTGGCAGCGCCGGGAGACGCTGGATCATGGCAGACCGAAACCAAGGGCTACCAATTTTGGACCAAAACAGATGCATATGGCTCGTTTAGCATCGAAAATGTACGCATCGGCGACTACAATCTTTATGCTTGGGTTCCTGGCTTCATTGGTGACTACCGTTACGACGTTGTCATTAGCGTCTCTGCAGGTAGTGATATGGACGTGGGGGATTTTGTTTTTGAGCCGCCTAGGAATGGTCCAACGCTATGGGAAATAGGCATTCCTGATCGCTCTGCAGCTGAATTCTATATTCCTGATCCTAACCCAAATTACATTAACAAATTATACGTCGACCATCCAGATAGATTCAGGCAATATGGACTGTGGGAGAGATATGCAGAATTATATCCTGATACAGACTTAGTATATACCGTTGGAGTTAGTGATTACACAAAAGATTGGTTCTTCGCTCATGTTAATCGGACCAGAAATGATGGGTCCCATTTACCAACCACGTGGCAAATCAAGTTTACACTCGATAACAACATAGATAAGAGCGGTATCTATAAATTGCGAGTGGCTTTGGCCACCGCCAATGTTGCTGAATTACAGATTCGGGTGAACAATCCAGAAGTAGAGTCGGCTCTATTTACGACAGGACAAATTGGGCACGATAATGCAATTGCAAGACATGGAATTCATGGACTCTATCGACTCTATAGTGTCGACATATCGGGATCTGTACTGGTGGAAGGAGAGAACACCATCTTGCTTTCACAAATTATAAACAATTCATCTTTCAACGGCGTCATGTACGATTATATCCGCCTCGAAGGCCCCCCATCCGACGTCTCTACACATCCATAA

Protein sequence

MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPMFLSAHYGGEELVLKLNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQIGHDNAIARHGIHGLYRLYSVDISGSVLVEGENTILLSQIINNSSFNGVMYDYIRLEGPPSDVSTHP

Homology

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BLAST of Cp4.1LG06g04070 vs. ExPASy Swiss-Prot
Match: Q8RJP2 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.7e-12
Identity = 87/365 (23.84%), Postives = 139/365 (38.08%), Query Frame = 0


Query: 78  AHYGGEELVLKLNPDEPWKKVFGPVFFYLNSVPNGTD------------------PLSLW 137
           +H+G  ++V +      ++K++GP   Y+N    G D                  P    
Sbjct: 261 SHFGTPDMVAQ----PGFEKLYGPWLLYIN---QGNDRELVADVSRRAEHERASWPYRWL 320

Query: 138 EDAKIQQE------QMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 197
           +DA+  ++      ++     +  +     A ++  +T GY F  +T+  G FS+ NV  
Sbjct: 321 DDARYPRQRATVSGRLRTEAPHATVVLNSSAENFDIQTTGYLFSARTNRDGRFSLSNVPP 380

Query: 198 GDYNLYAWVPG--FIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAE 257
           G+Y L A+  G   IG      V     G    +G      P   P  W IG  DR A E
Sbjct: 381 GEYRLSAYADGGTQIGLLAQQTV--RVEGKKTRLGQIDARQP--APLAWAIGQADRRADE 440

Query: 258 FYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNR 317
           F             + D P ++R    W+         DL + +G S   KDW++A    
Sbjct: 441 F------------RFGDKPRQYR----WQTEV----PADLTFEIGKSRERKDWYYAQTQ- 500

Query: 318 TRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPE----VESALF 377
                   P +W I F   N       Y L +A+A A+   +    ++P+    +   L 
Sbjct: 501 --------PGSWHILF---NTRTPEQPYTLNIAIAAASNNGMTTPASSPQLAVKLNGQLL 560

Query: 378 TTGQIGHDNAIARHGIH-GLYRLYSVDISGSVLVEGENTILLSQIINNSSFNGVMYDYIR 412
           TT +  +D +I R  +  G Y    + +    L +G N I L  +        VMYD I 
Sbjct: 561 TTLKYDNDKSIYRGAMQSGRYHEAHIPLPAGALQQGGNRITLELL-----GGMVMYDAIT 577

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.4e-10
Identity = 56/202 (27.72%), Postives = 86/202 (42.57%), Query Frame = 0


Query: 151 TKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVF 210
           +  YQ+W + D  G FS+++V+ G Y L  +  G  GD+  D V  V AG    + +  +
Sbjct: 381 SSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDGV-QVKAGKKTTIQE-TW 440

Query: 211 EPPRNGPTLWEIGIPDRSAAEF---YIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYP 270
           E    G  +W +G PD+S+ EF      DP        +  HP  +  Y     +   +P
Sbjct: 441 EAESAGTEIWRLGTPDKSSGEFRHGVARDPT-------HPLHPPEYLIYWGAYDWQSDFP 500

Query: 271 DTDLVYTVGVSDYTKDWFFAHVN---------RTRNDGSHLPTTWQIKFTL-DNNIDKSG 330
           D  + YT+G SD   D    H +         R   D +H    W I F L ++++ +  
Sbjct: 501 D-GINYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTH---DWTINFPLSEDDLAERS 560

Query: 331 IYKLRVALATANVAELQIRVNN 340
              L + LA A  A     V N
Sbjct: 561 KATLTIQLAGAKAASGNTDVYN 569

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BLAST of Cp4.1LG06g04070 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 9.8e-10
Identity = 76/291 (26.12%), Postives = 114/291 (39.18%), Query Frame = 0


Query: 154 YQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPP 213
           +Q+W   D  G FSI+ V  G Y L  +  G  GD+  D ++ V A     + +  ++P 
Sbjct: 381 HQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIV-VKARKSTSIKE-TWKPE 440

Query: 214 RNGPTLWEIGIPDRSAAEF---YIPDPNPNYINKLYVDHPDRFRQY-GLWERYAELYPDT 273
             G  +W +G PD+S+ EF      DP        +  HP  +  Y G ++  ++     
Sbjct: 441 SAGTEIWRLGTPDKSSGEFRHGAARDPT-------HPRHPPEYLIYWGAYDWQSDFPGGI 500

Query: 274 DLVYTVGVSDYTKDWFFAH--VNRTRNDG----SHLPTTWQIKFTLD-NNIDKSGIYKLR 333
           D  Y +G SD   D+   H  V     D     S+    W+I+F L    +       L 
Sbjct: 501 D--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLT 560

Query: 334 VALATANV--------------AELQIRVNNPEVESALFTTGQIGHD---NAIARHGIHG 393
           + LA A                A L +R    E E  L  T  IG+D   + I R  +  
Sbjct: 561 IQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEPL--TMVIGYDQSSSCIVRSAVSC 620

Query: 394 LYRLYSVDISGSVLVEGENTILLSQIINNSSFNG--------VMYDYIRLE 409
                  +   S L EG N + LS   N +++          V YD +RLE
Sbjct: 621 YQVREKWEFPASWLKEGSNLLRLSLPTNGTNYESAVLPTSVYVQYDALRLE 658

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BLAST of Cp4.1LG06g04070 vs. ExPASy Swiss-Prot
Match: B8NCU7 (Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglA PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 8.3e-09
Identity = 71/244 (29.10%), Postives = 108/244 (44.26%), Query Frame = 0


Query: 155 QFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRY-DVVISVSAGS--DMDVGDFVFE 214
           Q+WT T + GSF+   ++ GDY +      + G+Y+  +  +SV+AGS    D+  FV  
Sbjct: 303 QYWTYTSSDGSFTSPAMKPGDYTMVY----YQGEYKVAETSVSVTAGSSTSKDISGFV-- 362

Query: 215 PPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDL 274
               G T+++IG  D +   F       N  N+L + HP   R    W           L
Sbjct: 363 --ETGDTIFKIGDWDGTPTGF------RNAENQLRM-HPSDSRM-SSW---------GPL 422

Query: 275 VYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVA 334
            YTVG S+ T D+  A        G + P T  IKFT  +    +   ++   L+ A   
Sbjct: 423 TYTVGSSELT-DFPMAAFK-----GVNDPVT--IKFTATSAQTGAATLRIGTTLSFAG-G 482

Query: 335 ELQIRVNNPEVESALFTTGQIGHDNAIARHGIHGLYRLYSVDISGSVLVEGENTILLSQI 394
             Q  +N+ E  +    T    +   + R    GL  +Y V+I    +VEGENTI +S I
Sbjct: 483 RPQATINDYEGSAPSAPTNL--NSRGVTRGAYRGLGEVYDVNIPSGTIVEGENTITISVI 510

Query: 395 INNS 396
             +S
Sbjct: 543 SGSS 510

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy Swiss-Prot
Match: Q2U5P7 (Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.1e-08
Identity = 50/174 (28.74%), Postives = 75/174 (43.10%), Query Frame = 0


Query: 151 TKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYD-VVISVSAGSDMDVGDFV 210
           T+  Q+W + D  GSFSI  V  G Y +  +     G +  D V +  S   D     F 
Sbjct: 380 TESLQYWAEIDKSGSFSIPRVVEGTYRITIYADEIFGWFIQDHVKVLKSQSKDY---SFT 439

Query: 211 FEPPRNGPTLWEIGIPDRSAAEF---YIPDPNPNYINKLYVDHPDRFRQYGLWERYAELY 270
           ++    G  +W IGIPD+S+ E+   Y PD +           P++ R Y  W +Y   Y
Sbjct: 440 WKEESAGKEIWRIGIPDKSSGEYLHGYAPDTSKPL-------QPEQHRIY--WGKYD--Y 499

Query: 271 P---DTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPT--------TWQIKFTL 310
           P      + + VG SD ++D  + H     + G+HL T         W + F L
Sbjct: 500 PADFPEGINFHVGKSDPSQDLNYIHWAFFPSQGNHLRTEPYYDNVNNWTVTFDL 539

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BLAST of Cp4.1LG06g04070 vs. NCBI nr
Match: XP_023535231.1 (probable rhamnogalacturonate lyase B isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 847 bits (2188), Expect = 1.01e-306
Identity = 418/489 (85.48%), Postives = 418/489 (85.48%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 138 MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 197

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 198 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLK 257

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ                       
Sbjct: 258 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQMTKEVQSWPYSFPASENFPPPNQ 317

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 318 RGRLTGNLFVLDRYAKQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 377

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA
Sbjct: 378 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 437

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
           AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV
Sbjct: 438 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 497

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 418
           NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT
Sbjct: 498 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 557

[Close]

BLAST of Cp4.1LG06g04070 vs. NCBI nr
Match: XP_023535230.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 847 bits (2188), Expect = 2.03e-306
Identity = 418/489 (85.48%), Postives = 418/489 (85.48%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 157 MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 216

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 217 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLK 276

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ                       
Sbjct: 277 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQMTKEVQSWPYSFPASENFPPPNQ 336

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 337 RGRLTGNLFVLDRYAKQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 396

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA
Sbjct: 397 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 456

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
           AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV
Sbjct: 457 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 516

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 418
           NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT
Sbjct: 517 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 576

[Close]

BLAST of Cp4.1LG06g04070 vs. NCBI nr
Match: XP_022975861.1 (rhamnogalacturonate lyase B-like [Cucurbita maxima])

HSP 1 Score: 815 bits (2105), Expect = 8.67e-294
Identity = 401/489 (82.00%), Postives = 409/489 (83.64%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKR MPLP+DRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 157 MAIADNRKRSMPLPDDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 216

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 217 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLK 276

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           +NPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAK Q                       
Sbjct: 277 MNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKTQMTKEVQSWPYSFPASENFPPPNQ 336

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 337 RGRLTGNLFVLDRYVNQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 396

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDY YDVVISVSAGSD+D+GD VFEPPRNGPT+WEIGIPDRSA
Sbjct: 397 NVRIGDYNLYAWVPGFIGDYHYDVVISVSAGSDIDIGDLVFEPPRNGPTVWEIGIPDRSA 456

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
            EFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDY KDWFFAHV
Sbjct: 457 NEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYPKDWFFAHV 516

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 418
           NRTRNDGSHLPTTWQIKFTLDNN+DKSGIYKLRVALATANVAELQIRVNN EVE ALFTT
Sbjct: 517 NRTRNDGSHLPTTWQIKFTLDNNVDKSGIYKLRVALATANVAELQIRVNNLEVEHALFTT 576

[Close]

BLAST of Cp4.1LG06g04070 vs. NCBI nr
Match: XP_022936673.1 (probable rhamnogalacturonate lyase B [Cucurbita moschata])

HSP 1 Score: 810 bits (2091), Expect = 1.17e-291
Identity = 403/487 (82.75%), Postives = 405/487 (83.16%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKR MPLP+DRVG RGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 157 MAIADNRKRSMPLPDDRVGGRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 216

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 217 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLK 276

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           LN DEPWKKVFGPVFFYLNSVPNG DPL LWEDAKIQ                       
Sbjct: 277 LNADEPWKKVFGPVFFYLNSVPNGRDPLCLWEDAKIQMTKEVQSWPYSFPASQNFPPPNQ 336

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 337 RGRLTGNLYVLDTYVNQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 396

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGD VFEPPRNGPTLWEIGIPDRSA
Sbjct: 397 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDLVFEPPRNGPTLWEIGIPDRSA 456

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
           AEFYIPDPNPNYINKLYV HPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV
Sbjct: 457 AEFYIPDPNPNYINKLYVHHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 516

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 416
           NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVE ALFTT
Sbjct: 517 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVEPALFTT 576

[Close]

BLAST of Cp4.1LG06g04070 vs. NCBI nr
Match: KAG7024585.1 (rhiE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 802 bits (2072), Expect = 9.10e-289
Identity = 398/489 (81.39%), Postives = 405/489 (82.82%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKR MPLP+DRVG RGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 157 MAIADNRKRSMPLPDDRVGGRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 216

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 217 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALTMFLSAHYGGEELVLK 276

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           LN DEPWKKVFGPVFFYLNSVPNG DPL LWEDAKIQ                       
Sbjct: 277 LNADEPWKKVFGPVFFYLNSVPNGRDPLCLWEDAKIQMTKEVQSWPYSFPASQNFPPPNQ 336

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPA EAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 337 RGRLTGNLYVLDTYVNQEQMPAGEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 396

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGD VFEPPRNGPTLWEIGIP+RSA
Sbjct: 397 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDLVFEPPRNGPTLWEIGIPNRSA 456

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
           AEFYIPDPNPNYINKLYV HPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV
Sbjct: 457 AEFYIPDPNPNYINKLYVHHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 516

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 418
           NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVN+ EV+ ALFTT
Sbjct: 517 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNSSEVDPALFTT 576

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy TrEMBL
Match: A0A6J1IFC5 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV=1)

HSP 1 Score: 815 bits (2105), Expect = 4.20e-294
Identity = 401/489 (82.00%), Postives = 409/489 (83.64%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKR MPLP+DRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 157 MAIADNRKRSMPLPDDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 216

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 217 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLK 276

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           +NPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAK Q                       
Sbjct: 277 MNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKTQMTKEVQSWPYSFPASENFPPPNQ 336

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 337 RGRLTGNLFVLDRYVNQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 396

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDY YDVVISVSAGSD+D+GD VFEPPRNGPT+WEIGIPDRSA
Sbjct: 397 NVRIGDYNLYAWVPGFIGDYHYDVVISVSAGSDIDIGDLVFEPPRNGPTVWEIGIPDRSA 456

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
            EFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDY KDWFFAHV
Sbjct: 457 NEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYPKDWFFAHV 516

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 418
           NRTRNDGSHLPTTWQIKFTLDNN+DKSGIYKLRVALATANVAELQIRVNN EVE ALFTT
Sbjct: 517 NRTRNDGSHLPTTWQIKFTLDNNVDKSGIYKLRVALATANVAELQIRVNNLEVEHALFTT 576

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy TrEMBL
Match: A0A6J1FDW4 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 5.66e-292
Identity = 403/487 (82.75%), Postives = 405/487 (83.16%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNRKR MPLP+DRVG RGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV
Sbjct: 157 MAIADNRKRSMPLPDDRVGGRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 216

Query: 61  HGWISMDPSDDPP--------------------------------MFLSAHYGGEELVLK 120
           HGWISMDPSDDPP                                MFLSAHYGGEELVLK
Sbjct: 217 HGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLK 276

Query: 121 LNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ----------------------- 180
           LN DEPWKKVFGPVFFYLNSVPNG DPL LWEDAKIQ                       
Sbjct: 277 LNADEPWKKVFGPVFFYLNSVPNGRDPLCLWEDAKIQMTKEVQSWPYSFPASQNFPPPNQ 336

Query: 181 ----------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 240
                           QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE
Sbjct: 337 RGRLTGNLYVLDTYVNQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIE 396

Query: 241 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSA 300
           NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGD VFEPPRNGPTLWEIGIPDRSA
Sbjct: 397 NVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDLVFEPPRNGPTLWEIGIPDRSA 456

Query: 301 AEFYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 360
           AEFYIPDPNPNYINKLYV HPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV
Sbjct: 457 AEFYIPDPNPNYINKLYVHHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHV 516

Query: 361 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTT 416
           NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVE ALFTT
Sbjct: 517 NRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVEPALFTT 576

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy TrEMBL
Match: A0A6J1C5C7 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3 SV=1)

HSP 1 Score: 721 bits (1862), Expect = 2.76e-257
Identity = 355/480 (73.96%), Postives = 383/480 (79.79%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNR+R+MPLPEDR+  RGKTLDFPEAV LV+P+E  FKGEVDDKYQYSCENKDLKV
Sbjct: 156 MAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKV 215

Query: 61  HGWISMDP--------------SDDP--------------PMFLSAHYGGEELVLKLNPD 120
           HGWISMDP              S  P               MFLSAHY GE+LVLKLNPD
Sbjct: 216 HGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPD 275

Query: 121 EPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ--------------------------- 180
           EPWKKVFGPVF YLNSV +G DPLSLW+DAK Q                           
Sbjct: 276 EPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRL 335

Query: 181 ------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 240
                       Q+Q PA +AYVGLAAPG+AGSWQTE KGYQFWTKTDA GSFSIEN RI
Sbjct: 336 TGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENKGYQFWTKTDADGSFSIENARI 395

Query: 241 GDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFY 300
           GDYNLYAWVPGFIGDYRYDV++S+SAGSDMDVGD V+EPPRNGPTLWEIGIPDRSAAEFY
Sbjct: 396 GDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY 455

Query: 301 IPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTR 360
           +PDP+PNYINKLYVDHPDRFRQYGLWERYAELYPD DLVYTVGVSDYTKDWFFAHVNRTR
Sbjct: 456 VPDPDPNYINKLYVDHPDRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTR 515

Query: 361 NDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQIG 413
           NDGSHL TTWQIKFT+DN +DK+  Y LRVALATANVAELQI+VN+PE + A+FTTGQIG
Sbjct: 516 NDGSHLSTTWQIKFTVDN-VDKNDAYTLRVALATANVAELQIQVNDPEAKPAVFTTGQIG 575

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy TrEMBL
Match: A0A1S3CQT8 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 1.38e-256
Identity = 356/480 (74.17%), Postives = 383/480 (79.79%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIAD+R+R+MPLPEDR+  RGK LDFPEAVLLV+P+E  FKGEVDDKYQYSCENKDLKV
Sbjct: 156 MAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKV 215

Query: 61  HGWISMDP--------------SDDP--------------PMFLSAHYGGEELVLKLNPD 120
           HGWISMDP              S  P               MFLSAHY GE+LVLKL+PD
Sbjct: 216 HGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPD 275

Query: 121 EPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ--------------------------- 180
           EPWKKVFGPVFFYLNSV +G DPLSLWEDAK Q                           
Sbjct: 276 EPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQMSIEVQSWPYSFPASEDFPPPNQRGRL 335

Query: 181 ------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 240
                       +E+MPA +AYVGLAAPG+AGSWQTE KGYQFWTKTDA GSFSIE+VRI
Sbjct: 336 TGKLFVLDRYINEEKMPATDAYVGLAAPGEAGSWQTENKGYQFWTKTDADGSFSIEHVRI 395

Query: 241 GDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFY 300
           GDYNLYAWVPGFIGDYRYDV+I+VS GSDMDVGD V+EPPRNGPTLWEIGIPDRSAAEFY
Sbjct: 396 GDYNLYAWVPGFIGDYRYDVLINVSPGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY 455

Query: 301 IPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTR 360
           IPDP+P YINKLYV+HPDRFRQYGLWERYAELYPD DLVY+VGVSDYTKDWFFAHVNR R
Sbjct: 456 IPDPDPKYINKLYVNHPDRFRQYGLWERYAELYPDADLVYSVGVSDYTKDWFFAHVNRKR 515

Query: 361 NDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQIG 413
           NDGSHL TTW+IKF +DN IDKSG Y LRVALATANVAELQI+VNNPE + +LFTTG IG
Sbjct: 516 NDGSHLATTWEIKFRVDNIIDKSGTYTLRVALATANVAELQIQVNNPEAKPSLFTTGMIG 575

[Close]

BLAST of Cp4.1LG06g04070 vs. ExPASy TrEMBL
Match: A0A0A0LGY0 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 7.52e-254
Identity = 351/481 (72.97%), Postives = 382/481 (79.42%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIAD+R+R+MPLPEDR+  RGK LDFPEAVLLV+P+E  FKGEVDDKYQYSCENKDLKV
Sbjct: 156 MAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKV 215

Query: 61  HGWISMDP--------------SDDP--------------PMFLSAHYGGEELVLKLNPD 120
           HGWISMDP              S  P               MFLSAHY GE+LVLK++PD
Sbjct: 216 HGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKVSPD 275

Query: 121 EPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQ--------------------------- 180
           EPWKKVFGPVFFYLNSV +G DPLSLWEDAK Q                           
Sbjct: 276 EPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQMSKEVQSWPYSFPASEDFPPPNQRGRL 335

Query: 181 ------------QEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 240
                       +E+MPA +AYVGLAAPGD GSWQTE KGYQFWTKTDA GSFSIE+VRI
Sbjct: 336 TGKLFVLDRYVNEEKMPATDAYVGLAAPGDVGSWQTEKKGYQFWTKTDADGSFSIEHVRI 395

Query: 241 GDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFY 300
           GDYNLYAWVPGFIGDYRYDV+I+VS+GSDMDVGD V+EPPRNGPTLWEIGIPDRSAAEFY
Sbjct: 396 GDYNLYAWVPGFIGDYRYDVLINVSSGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY 455

Query: 301 IPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTR 360
           IPDP+P YINKLY++HPDRFRQYGLWE+YAEL+PD DLVY+VGVSDYTKDWFFAHVNR R
Sbjct: 456 IPDPDPKYINKLYINHPDRFRQYGLWEKYAELHPDADLVYSVGVSDYTKDWFFAHVNRKR 515

Query: 361 NDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQIG 414
           NDGSHL TTW+IKF LDN IDK   Y LRVALATANVAELQI+VNNPE + +LFTTG IG
Sbjct: 516 NDGSHLATTWEIKFRLDNIIDKGVSYTLRVALATANVAELQIQVNNPEAKPSLFTTGVIG 575

[Close]

BLAST of Cp4.1LG06g04070 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 567.4 bits (1461), Expect = 9.6e-162
Identity = 277/488 (56.76%), Postives = 347/488 (71.11%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAIADNR+R MPLPEDR+G+RG+ L +PEAVLLVHP E  FKGEVDDKY+YS ENKDLKV
Sbjct: 160 MAIADNRQRKMPLPEDRLGKRGRPLAYPEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKV 219

Query: 61  HGWISMD---------PSDD-------------------PPMFLSAHYGGEELVLKLNPD 120
           HGWIS +         PS++                     MFLSAHY GE++V+K+   
Sbjct: 220 HGWISHNLDLGCWQIIPSNEFRSGGLSKQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAG 279

Query: 121 EPWKKVFGPVFFYLNSVPNGT-DPLSLWEDAK---------------------------- 180
           + WKKVFGPVF YLN +P+ T DPLSLW+DAK                            
Sbjct: 280 DSWKKVFGPVFTYLNCLPDKTSDPLSLWQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGC 339

Query: 181 -----------IQQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVR 240
                      +  + +PA  A+VGLA PG+ GSWQ E+KGYQFWT+ D+ G F+I ++R
Sbjct: 340 ISGRLLVCDKFLSDDFLPANGAFVGLAPPGEVGSWQLESKGYQFWTEADSDGYFAINDIR 399

Query: 241 IGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEF 300
            G+YNL  +V G+IGDY+Y+ +I+++AG D+DVG+ V+EPPR+GPT+WEIGIPDRSAAEF
Sbjct: 400 EGEYNLNGYVTGWIGDYQYEQLINITAGCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEF 459

Query: 301 YIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRT 360
           ++PDPNP YINKLY+ HPDRFRQYGLWERY ELYP  DLV+T+GVSDY KDWFFAHV R 
Sbjct: 460 FVPDPNPKYINKLYIGHPDRFRQYGLWERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRK 519

Query: 361 RNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVE--SALFTTG 418
             D ++  TTWQIKF L+ N+ KS  YK+R+ALATANVAELQ+R+N+ + E  + +FTTG
Sbjct: 520 MGDDTYQKTTWQIKFKLE-NVQKSCTYKIRIALATANVAELQVRMNDDDTEKTTPIFTTG 579

[Close]

BLAST of Cp4.1LG06g04070 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 521.5 bits (1342), Expect = 6.0e-148
Identity = 263/479 (54.91%), Postives = 320/479 (66.81%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MA+ D+R+R+MPLP+DR+  RG+ L +PEAVLLV+P E+ FKGEVDDKYQYSCENKD+ V
Sbjct: 140 MAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQFKGEVDDKYQYSCENKDITV 199

Query: 61  HGWISMD----------PSDD-------------------PPMFLSAHYGGEELVLKLNP 120
           HGWI  +          PS +                     +F+SAHY GE+LV K + 
Sbjct: 200 HGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTALAVFISAHYTGEDLVPKFSE 259

Query: 121 DEPWKKVFGPVFFYLN-SVPNGTDPLSLWEDAK----IQQEQMP---------------- 180
            E WKKVFGPVF YLN S  +  DPL LW+DAK    ++ E  P                
Sbjct: 260 GEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAESWPYSFPASDDYVKTEQRG 319

Query: 181 -------------------AAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENV 240
                              A   YVGLA PG AGSWQ E K YQFWT+TD  G F I  +
Sbjct: 320 NVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECKEYQFWTRTDEEGFFYISGI 379

Query: 241 RIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAE 300
           R G YNLYAW+PGFIGDY+YD VI++++G  + V D V++PPRNG TLWEIG PDRSAAE
Sbjct: 380 RPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQPPRNGATLWEIGFPDRSAAE 439

Query: 301 FYIPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNR 360
           FY+PDPNP YIN LY +HPDRFRQYGLWERYAELYPD DLVY VG SDY KDWF+A V R
Sbjct: 440 FYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDLVYVVGSSDYRKDWFYAQVTR 499

Query: 361 TRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQ 411
            +++ ++  TTWQIKF L  NIDK+  Y LRVA+A+A  +ELQIRVNN    S +FT+G 
Sbjct: 500 KKDNKTYQGTTWQIKFEL-KNIDKNHSYTLRVAIASATFSELQIRVNNANA-SPMFTSGL 559

[Close]

BLAST of Cp4.1LG06g04070 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 5.7e-146
Identity = 253/480 (52.71%), Postives = 314/480 (65.42%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAI+D+R+R+MP+P+DRV  RG+ L +PEAV L+ P E  FKGEVDDKY+YS E+KD+KV
Sbjct: 145 MAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLDPIEPEFKGEVDDKYEYSMESKDIKV 204

Query: 61  HGWISMDPS----------------------------DDPPMFLSAHYGGEELVLKLNPD 120
           HGWIS + S                             +  +F S HY G +L++     
Sbjct: 205 HGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHVGPTNLAVFHSTHYVGADLIMSFKNG 264

Query: 121 EPWKKVFGPVFFYLNSVPNGTDPLSLWEDAK----------------------------- 180
           E WKKVFGPVF YLNS P G DPL LW +AK                             
Sbjct: 265 EAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTKIEEEKWPYNFTASDDFPASDQRGSV 324

Query: 181 ----------IQQEQMPAAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 240
                     I  E +PA  +YVGLAAPGD GSWQ E KGYQFW+K D  GSFSI NVR 
Sbjct: 325 SGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQRECKGYQFWSKADENGSFSINNVRS 384

Query: 241 GDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFY 300
           G YNLYA+ PGFIGDY  D V  +S GS + +GD V+EPPR+G TLWEIG+PDRSAAEFY
Sbjct: 385 GRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDLVYEPPRDGSTLWEIGVPDRSAAEFY 444

Query: 301 IPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTR 360
           IPDPNP+++NKLY++H D++RQYGLWERY+ELYPD D+VY V + DY+K+WFF  V R +
Sbjct: 445 IPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPDEDMVYNVDIDDYSKNWFFMQVTRKQ 504

Query: 361 NDGSHLPTTWQIKFTLDNNI-DKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQI 413
            +G +  TTWQI+F  D+ + + +G +KLR+ALAT+NVAELQ+RVN+   +  LF T QI
Sbjct: 505 ANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALATSNVAELQVRVNDLSADPPLFRTEQI 564

[Close]

BLAST of Cp4.1LG06g04070 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.3e-145
Identity = 258/479 (53.86%), Postives = 314/479 (65.55%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MA+AD+RKR MP P+D    R +TLD+ EA LL  P +   +GEVDDKYQYSCENKDL+V
Sbjct: 199 MAVADDRKRIMPFPDDLCKGRCQTLDYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRV 258

Query: 61  HGWISMDP--------------SDDP--------------PMFLSAHYGGEELVLKLNPD 120
           HGWIS DP              S  P               +F S HY G+ ++ +    
Sbjct: 259 HGWISFDPPVGFWQITPSNEFRSGGPLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHG 318

Query: 121 EPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQQ----EQMP------------------ 180
           EPWKKV+GPVF YLNS  NG DPL LW+DAKI+     E+ P                  
Sbjct: 319 EPWKKVYGPVFIYLNSTANGDDPLCLWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTA 378

Query: 181 -----------------AAEAYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 240
                            A  AYVGLA PGD+GSWQ E KGYQFW   D  G FSI NVR 
Sbjct: 379 RGRLLIRDRFINNDLISARGAYVGLAPPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRP 438

Query: 241 GDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFY 300
           G+YNLYAWVP FIGDY    ++ V++G  +++GD V+EPPR+GPTLWEIGIPDR A+EF+
Sbjct: 439 GEYNLYAWVPSFIGDYHNGTIVRVTSGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFF 498

Query: 301 IPDPNPNYINKLYVDHPDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTR 360
           IPDP+P  +N++ V H DRFRQYGLW++Y ++YP+ DLVYTVGVSDY +DWFFAHV R +
Sbjct: 499 IPDPDPTLVNRVLVHHQDRFRQYGLWKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPRKK 558

Query: 361 NDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQIG 413
            D  H  TTWQI F L+ NID+   YKLRVA+A+A +AELQIR+N+ E    LFTTG IG
Sbjct: 559 GD-VHEGTTWQIIFNLE-NIDQKANYKLRVAIASATLAELQIRINDAEAIRPLFTTGLIG 618

[Close]

BLAST of Cp4.1LG06g04070 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.2e-137
Identity = 253/478 (52.93%), Postives = 307/478 (64.23%), Query Frame = 0


Query: 1   MAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKV 60
           MAI+D+R+R MP   DR     K+L + EAVLL +P    FKGEVDDKY YS E+KD  V
Sbjct: 201 MAISDDRQRSMPSMADR--ENSKSLAYKEAVLLTNPSNPMFKGEVDDKYMYSMEDKDNNV 260

Query: 61  HGWISMD---------PSDD-------------------PPMFLSAHYGGEELVLKLNPD 120
           HGWIS D         PSD+                     MF S HY G+E+ +     
Sbjct: 261 HGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGPITLSMFTSTHYAGKEMRMDYRNG 320

Query: 121 EPWKKVFGPVFFYLNSVPNGTDPLSLWEDAK--------------IQQEQMPAAE----- 180
           EPWKKVFGPV  YLNSV      L LW DAK              I  E  P        
Sbjct: 321 EPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEVKSWPYDFITSEDYPLRHQRGTL 380

Query: 181 --------------------AYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRI 240
                               A+VGLA  G+AGSWQTE+KGYQFWTK D  G F IENVR 
Sbjct: 381 EGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTESKGYQFWTKADRRGRFIIENVRA 440

Query: 241 GDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFY 300
           G+Y+LYAW  GFIGDY+Y+  I+++ GS+M+VG  V+EPPRNGPTLWEIG+PDR+A EFY
Sbjct: 441 GNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYEPPRNGPTLWEIGVPDRTAGEFY 500

Query: 301 IPDPNPNYINKLYVDH-PDRFRQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRT 360
           IPDP P  +NKLYV+   DRFRQYGLW+RYA+LYP  DLVYT+GVSDY  DWFFAHV R 
Sbjct: 501 IPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQNDLVYTIGVSDYRSDWFFAHVARN 560

Query: 361 RNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQI 411
             + ++ PTTWQI F L  N+++ G Y LR+ALA+A  +ELQIR+N+P+   A+FTTG I
Sbjct: 561 VGNDTYQPTTWQIIFNL-KNVNRIGRYTLRIALASAADSELQIRINDPK-SDAIFTTGFI 620

The following BLAST results are available for this feature:

BLAST of Cp4.1LG06g04070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RJP2	2.7e-12	23.84	Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE...	[more]
A5ABH4	3.4e-10	27.72	Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F...	[more]
Q5AZ85	9.8e-10	26.12	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ...	[more]
B8NCU7	8.3e-09	29.10	Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 /...	[more]
Q2U5P7	4.1e-08	28.74	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / ...	[more]

BLAST of Cp4.1LG06g04070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023535231.1	1.01e-306	85.48	probable rhamnogalacturonate lyase B isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023535230.1	2.03e-306	85.48	probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022975861.1	8.67e-294	82.00	rhamnogalacturonate lyase B-like [Cucurbita maxima]	[more]
XP_022936673.1	1.17e-291	82.75	probable rhamnogalacturonate lyase B [Cucurbita moschata]	[more]
KAG7024585.1	9.10e-289	81.39	rhiE [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cp4.1LG06g04070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IFC5	4.20e-294	82.00	Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV...	[more]
A0A6J1FDW4	5.66e-292	82.75	Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 ...	[more]
A0A6J1C5C7	2.76e-257	73.96	Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3...	[more]
A0A1S3CQT8	1.38e-256	74.17	Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1	[more]
A0A0A0LGY0	7.52e-254	72.97	Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=...	[more]

BLAST of Cp4.1LG06g04070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24430.1	9.6e-162	56.76	Rhamnogalacturonate lyase family protein	[more]
AT1G09890.1	6.0e-148	54.91	Rhamnogalacturonate lyase family protein	[more]
AT1G09880.1	5.7e-146	52.71	Rhamnogalacturonate lyase family protein	[more]
AT1G09910.1	1.3e-145	53.86	Rhamnogalacturonate lyase family protein	[more]
AT2G22620.1	2.2e-137	52.93	Rhamnogalacturonate lyase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029411	Rhamnogalacturonan lyase, domain III	PFAM	PF14683	CBM-like	coord: 218..407 e-value: 2.6E-54 score: 183.6
None	No IPR available	GENE3D	2.60.120.260		coord: 216..408 e-value: 3.9E-20 score: 74.1
None	No IPR available	GENE3D	2.60.40.1120		coord: 128..205 e-value: 4.1E-8 score: 35.3
None	No IPR available	PANTHER	PTHR32018:SF16	RHAMNOGALACTURONATE LYASE B-RELATED	coord: 74..126
None	No IPR available	PANTHER	PTHR32018	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 1..69 coord: 124..415 coord: 74..126
None	No IPR available	PANTHER	PTHR32018:SF16	RHAMNOGALACTURONATE LYASE B-RELATED	coord: 1..69 coord: 124..415
None	No IPR available	CDD	cd10317	RGL4_C	coord: 220..408 e-value: 1.87881E-58 score: 186.712
IPR010325	Rhamnogalacturonate lyase	PFAM	PF06045	Rhamnogal_lyase	coord: 1..45 e-value: 8.5E-13 score: 48.2
IPR029413	Rhamnogalacturonan lyase, domain II	PFAM	PF14686	fn3_3	coord: 133..203 e-value: 8.1E-24 score: 83.2
IPR029413	Rhamnogalacturonan lyase, domain II	CDD	cd10316	RGL4_M	coord: 126..207 e-value: 5.82542E-29 score: 106.564
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 215..408
IPR013784	Carbohydrate-binding-like fold	SUPERFAMILY	49452	Starch-binding domain-like	coord: 149..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG06g04070.1	Cp4.1LG06g04070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0102210	rhamnogalacturonan endolyase activity