Cp4.1LG06g03580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g03580
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAspartate--tRNA ligase
LocationCp4.1LG06: 2108248 .. 2112422 (+)
RNA-Seq ExpressionCp4.1LG06g03580
SyntenyCp4.1LG06g03580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATTTGTCCAGCCCATCTTACCAGCGCAGAGGTTTATTGCGCTTCCTTCTTCGTTACAATCTCTCCTTCATTGCCCTCAATATTCCTTCCCATTTCTTCATCTTCTTCCTGCAACCATGTCCGCTTCATCCGAAACTCCGATCGATGAGCCCCTGTCTGCTGGTGACGAAGCATCTAAAGCTGTCAGCAAGAAGGCCGCCAAGAAGGAGGCTGCCAAGCTGGAAAAGCTGCGCCGTCGCCAGGAAGCAGTCGCCGAAGCTGCTATTTCGTCTCTCAATGTCGAGGACGACCCACTCTCCGCTAACTATGGCGATGTTCCTCTCTCCGATCTCCAATCCAAGGAGGCTAAGGATGTCGCGAACTGGACTCAGGTTGGCTCTTTGACGGGGGAGCTTAAAGACAAGTATGTGATTCTACGGGGACGAATTCAGACGATCCGTGCTGTTGCCAAGAAGATGGCGTTTCTCGTGGTGAGAGAAAAGGGATTCACCGTGCAGTGCGTTGTATCGGAGCAGCCGGAACTCGTCAGTCGCCAGATGGTGAAGTATGTCGTTGGATTGAGCCGTGAATCCATTGTCGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTACGCAGCAGGTGGAGATTCAAGTGAGGAAAGTATACTGCATCAGTAAGGCAATGCCGAGTCTGCCTATAAATATTGAGGATGCTGCTCGTAGTGAAGCTGAGATTGAGAAAGCGTTGCAGGTATACTTCGTTATCGCTCACTTTCGATGTGTTCTTCTTCGTACTTCGATTATCTGTTCAGCTCGATTGTCTAATTTCCATTGTTCTTCGTAATTAATTTGGAGAATTTTAGAAGTTCCAAATCTAATGATTGTATTTTCTTTCATTCTATGTCTATTGAAGCTAATTTTTTATACCAAAAATAAAATAAGAATTTTCTAGAAATTACACATCCTGTTTAACGTCGGACTCATTTGTTGGCTGCAGGCTGGTGAGCAACTAGTACGTGTTAATCAGGATACGCGGTTGAACTACAGAGTTCTTGACATGCGAACACCAGCTAATCAAGGAATCTTTCGCATTCAATGTCAAGTTGGAACAGTGAGTTTTCTATCTTATTTTCTGTTTTCTGGTTCTAATTGTTTCATTTTTTTAAAATTATTAGAACAATTTCATTGATGTCATTACAAAAGAGGGGAGACCCCTGTTGGGAGATAACCAAAAACCTCTCCAATTGAAGGAGGGAGGTTAAGCTATAGTGATGAAAAAGAACCTTTACACCAAGTCACATCCAACTGAACTTTAGCATAAAAAAATCTACTAAAAAAGTATTCCTTAACTTGGAAAATTATTTTGTTCCTATCTTGCCAGTTACTACAGCAAAATTCCCTAATAAAATTCACCCACAGAAGCTTTTTTTTTTTTCATTTTTGAAATGATGACCCCCTAAAGTATGCGAGAGAAGAAGCTTTAGATTTTCAAATCCACTGTGCTAGAAAGGTGCCATTTCTTTTTTCGTGGGAGGATTTTTTTTTTTTTTTTTTCATTTAATGAGATGCAGCCCCTTACTTTCTCCATGGCCTTTCGAAATGAAATTTCTAATCACTCTCTATGGTTGAGGCCACCTTGACCAGTGTTTTAAATACAAACTTATAGTACGTAGGAAGTTTGATAGTGTGGCTTGGATGAGCGTAGGCCTTCCCCCCTTTGTTATATGTGTATTTTTCCAAGAGAGAAGCCTCTTGCCCACCTTTTCAGTAATGGGCTTCCAAAATTGTAGTGACCGAGGCTTGCCATTGAAGGGAAGACCAAGATAAACGGCGGGCCAATTTCCCACTTTGCAACAATATTTATTTGCAAGATCATGCACCTTCTGTATTTCCATATTTATGCCTAAGATTTCTGATTTTTTATGATTGACATTGAGCCCTGATGCCATTTCAAAATGCCTCACAACATTGAATAAGTTATTGAGAGCTTGTTCTTTTGCCCCGAGAATAAGATTGTATTGTCATTAAAGGTTTCTTTTAATTGTTGATCTAAATGGATGTTTCATATCATACCAAGTTGAAGCATACAGGCTTTATTCAAAGTTGACCATGATAGCTATATCTACTATTATCAGCGAGCTTGATTCGTGTTGCATAGTTCGACGGTCATCTTTTGAATAAAAAACTATTGACTGGTGGATCTTTGGTAGTTTCATCCATTAATAGTTGGAGTCTTTGATGGCGTCCACGTTTAGTATCATCAGCATAGCTTTCTTACTTGGTATCCATGATTTTTCCATGCAATGTCTTGTTTGATTTGTAGATTTATTAAGGGGATTGATGAGGCATGTTTACAGCTAGTGTTTGAGTCCTTAGCATTATTGTAAATGAAGGGTTTGCAACATAGATTGAGTTTGTTCTCATTATGCAGATATTTCGACAATTTCTGTTGTCCGAAGGCTTTGTTGAAATCCATACACCAAAATTGATTGGGGGTTCCAGTGAAGGTGGTGCTTCTGTATTTAGGTTGGACTACAAAGGTCAACCGGCTTGCCTGGCTCAGTCACCTCAGCTTCATAAGCAAATGGCAATCTGCGGCGACTTCGGCCGTGTATTTGAGATTGGTCCTGTTTTCAGAGCAGAGGACTCATTCACACATAGACATTTATGTGAATTTACAGGACTTGATGTTGAAATGGAGATCAAAACCCATTATTCTGAGGTAATGCTCTGAGTTGACTTTTTATTTGTCATGGTCATCTTTACTTGATACTTGATCTGATTTCTTTTCTTTTTCTATGCAGGTCATGGACATCGTAGACCGATTATTTGTCTCAATGTTTGACAGTATGAATCAAAACTGTAAGAAGGAGCTTGAAGCAATTGATAAACAGTATCCCTTTGAGCCTTTGAAGGTGAACTTTATTGATAACCTACTTCCCAGAAATCTTTATAGTGTCGAAGTGTTGGATAATCTTGTGGCTTTGGATTTATGCCAATAAATATGTAAAATGTGGATAGATGACTGAGTTTGGTAGTAGATTGATTTCTTCTCATTTCCATCTTTGATCCGAGTTCATGATTATGCAGTACTTGCGAAACACTCTACGCCTTACATTTGAGGAAGGAATTCAGATGTTGAAGGTGAAGTTCTCTTGTTTGTTTTTTGTAACTTTTTAATTGCAGTGCCAGATAAATTGTAGAAAGATCCCAAATCCCAAATGTCATGACATTTTCTTCTTGGTTATGAATGATTATCTACACAGGATGCTGGCGTTGAAATCGATCCTCTCGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGACGACTCGTCCTAGAGAAGTAATTCGTGTCCCTACTTCGAACTTCTTATGATGTTGTACAATTATTATATATCTAAAGCTTGCTGATCAAACTTTTCTAACTATAGGTATGGCACAGAGTTCTACATTCTTCACCGCTATCCTTTGTCTGTTAGGCCATTCTACACTATGCCTTGCCATGATAACCCAACTTACAGTAACTCATTTGATGTCTTTATTCGAGGTATACATTAAATATTATTTCATCACCATAAGTGTCTTGCTGGTGCTTATTCTTCTCTATCATTTTATGTAACAAAATTTTCTAATATATGATCCATGATAAGTAACTTAAAACTTTGTTGTTCACGCAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTTCACGTGCCAGAATTTCTTGCAGAACGCGCTCAAGCATGTGGGATCGATGTAAAAACCATAGAAACTTACATCGATTCGTTTAGGTATGTTTCTCTTATGCTTTTGTTTTCTTTTCCCCTTTACTCAAAGTTGAAATTTAATCGAATAAACGAAATCTAACTGTGCAATGCTTTCATTGCAGGTATGGCGCTCCCCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTAGTGATGCTTTTTTGTGGTTTGAATAACATTCGGAAAACATCATTGTTCCCTCGCGACCCACAGAGGATTGCTCCTTAGAATTGCACCAGTAATAATGTAATTCCCGTTTTTGAAATTGACATTTTGTTGCCTCTTTCGCACTTCATAGGGAAAATATGAATTTCAGATCTCTGCAGGATGGTGTATTGAATACAATTGTTTGCAACGTTATAATGCAAAATTTGTTTTGACCAG

mRNA sequence

ATGGTATTTAGGTTTATTGCGCTTCCTTCTTCGTTACAATCTCTCCTTCATTGCCCTCAATATTCCTTCCCATTTCTTCATCTTCTTCCTGCAACCATGTCCGCTTCATCCGAAACTCCGATCGATGAGCCCCTGTCTGCTGGTGACGAAGCATCTAAAGCTGTCAGCAAGAAGGCCGCCAAGAAGGAGGCTGCCAAGCTGGAAAAGCTGCGCCGTCGCCAGGAAGCAGTCGCCGAAGCTGCTATTTCGTCTCTCAATGTCGAGGACGACCCACTCTCCGCTAACTATGGCGATGTTCCTCTCTCCGATCTCCAATCCAAGGAGGCTAAGGATGTCGCGAACTGGACTCAGGTTGGCTCTTTGACGGGGGAGCTTAAAGACAAGTATGTGATTCTACGGGGACGAATTCAGACGATCCGTGCTGTTGCCAAGAAGATGGCGTTTCTCGTGGTGAGAGAAAAGGGATTCACCGTGCAGTGCGTTGTATCGGAGCAGCCGGAACTCGTCAGTCGCCAGATGGTGAAGTATGTCGTTGGATTGAGCCGTGAATCCATTGTCGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTACGCAGCAGGTGGAGATTCAAGTGAGGAAAGTATACTGCATCAGTAAGGCAATGCCGAGTCTGCCTATAAATATTGAGGATGCTGCTCGTAGTGAAGCTGAGATTGAGAAAGCGTTGCAGGCTGGTGAGCAACTAGTACGTGTTAATCAGGATACGCGGTTGAACTACAGAGTTCTTGACATGCGAACACCAGCTAATCAAGGAATCTTTCGCATTCAATGTCAAATATTTCGACAATTTCTGTTGTCCGAAGGCTTTGTTGAAATCCATACACCAAAATTGATTGGGGGTTCCAGTGAAGGTGGTGCTTCTGTATTTAGGTTGGACTACAAAGGTCAACCGGCTTGCCTGGCTCAGTCACCTCAGCTTCATAAGCAAATGGCAATCTGCGGCGACTTCGGCCGTGTATTTGAGATTGGTCCTGTTTTCAGAGCAGAGGACTCATTCACACATAGACATTTATGTGAATTTACAGGACTTGATGTTGAAATGGAGATCAAAACCCATTATTCTGAGGTCATGGACATCGTAGACCGATTATTTGTCTCAATGTTTGACAGTATGAATCAAAACTGTAAGAAGGAGCTTGAAGCAATTGATAAACAGTATCCCTTTGAGCCTTTGAAGTACTTGCGAAACACTCTACGCCTTACATTTGAGGAAGGAATTCAGATGTTGAAGGATGCTGGCGTTGAAATCGATCCTCTCGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGACGACTCGTCCTAGAGAAGTATGGCACAGAGTTCTACATTCTTCACCGCTATCCTTTGTCTGTTAGGCCATTCTACACTATGCCTTGCCATGATAACCCAACTTACAGTAACTCATTTGATGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTTCACGTGCCAGAATTTCTTGCAGAACGCGCTCAAGCATGTGGGATCGATGTAAAAACCATAGAAACTTACATCGATTCGTTTAGGTATGGCGCTCCCCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTAGTGATGCTTTTTTGTGGTTTGAATAACATTCGGAAAACATCATTGTTCCCTCGCGACCCACAGAGGATTGCTCCTTAGAATTGCACCAGTAATAATGTAATTCCCGTTTTTGAAATTGACATTTTGTTGCCTCTTTCGCACTTCATAGGGAAAATATGAATTTCAGATCTCTGCAGGATGGTGTATTGAATACAATTGTTTGCAACGTTATAATGCAAAATTTGTTTTGACCAG

Coding sequence (CDS)

ATGGTATTTAGGTTTATTGCGCTTCCTTCTTCGTTACAATCTCTCCTTCATTGCCCTCAATATTCCTTCCCATTTCTTCATCTTCTTCCTGCAACCATGTCCGCTTCATCCGAAACTCCGATCGATGAGCCCCTGTCTGCTGGTGACGAAGCATCTAAAGCTGTCAGCAAGAAGGCCGCCAAGAAGGAGGCTGCCAAGCTGGAAAAGCTGCGCCGTCGCCAGGAAGCAGTCGCCGAAGCTGCTATTTCGTCTCTCAATGTCGAGGACGACCCACTCTCCGCTAACTATGGCGATGTTCCTCTCTCCGATCTCCAATCCAAGGAGGCTAAGGATGTCGCGAACTGGACTCAGGTTGGCTCTTTGACGGGGGAGCTTAAAGACAAGTATGTGATTCTACGGGGACGAATTCAGACGATCCGTGCTGTTGCCAAGAAGATGGCGTTTCTCGTGGTGAGAGAAAAGGGATTCACCGTGCAGTGCGTTGTATCGGAGCAGCCGGAACTCGTCAGTCGCCAGATGGTGAAGTATGTCGTTGGATTGAGCCGTGAATCCATTGTCGACGTTGAAGGAGTTGTGTCTGTGCCCAATGTTGCCATTAAAGGTGCTACGCAGCAGGTGGAGATTCAAGTGAGGAAAGTATACTGCATCAGTAAGGCAATGCCGAGTCTGCCTATAAATATTGAGGATGCTGCTCGTAGTGAAGCTGAGATTGAGAAAGCGTTGCAGGCTGGTGAGCAACTAGTACGTGTTAATCAGGATACGCGGTTGAACTACAGAGTTCTTGACATGCGAACACCAGCTAATCAAGGAATCTTTCGCATTCAATGTCAAATATTTCGACAATTTCTGTTGTCCGAAGGCTTTGTTGAAATCCATACACCAAAATTGATTGGGGGTTCCAGTGAAGGTGGTGCTTCTGTATTTAGGTTGGACTACAAAGGTCAACCGGCTTGCCTGGCTCAGTCACCTCAGCTTCATAAGCAAATGGCAATCTGCGGCGACTTCGGCCGTGTATTTGAGATTGGTCCTGTTTTCAGAGCAGAGGACTCATTCACACATAGACATTTATGTGAATTTACAGGACTTGATGTTGAAATGGAGATCAAAACCCATTATTCTGAGGTCATGGACATCGTAGACCGATTATTTGTCTCAATGTTTGACAGTATGAATCAAAACTGTAAGAAGGAGCTTGAAGCAATTGATAAACAGTATCCCTTTGAGCCTTTGAAGTACTTGCGAAACACTCTACGCCTTACATTTGAGGAAGGAATTCAGATGTTGAAGGATGCTGGCGTTGAAATCGATCCTCTCGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGACGACTCGTCCTAGAGAAGTATGGCACAGAGTTCTACATTCTTCACCGCTATCCTTTGTCTGTTAGGCCATTCTACACTATGCCTTGCCATGATAACCCAACTTACAGTAACTCATTTGATGTCTTTATTCGAGGGGAAGAAATCATTTCTGGGGCTCAGCGTGTTCACGTGCCAGAATTTCTTGCAGAACGCGCTCAAGCATGTGGGATCGATGTAAAAACCATAGAAACTTACATCGATTCGTTTAGGTATGGCGCTCCCCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTAGTGATGCTTTTTTGTGGTTTGAATAACATTCGGAAAACATCATTGTTCCCTCGCGACCCACAGAGGATTGCTCCTTAG

Protein sequence

MVFRFIALPSSLQSLLHCPQYSFPFLHLLPATMSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPLSANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVREKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDSMNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Homology
BLAST of Cp4.1LG06g03580 vs. ExPASy Swiss-Prot
Match: Q9M084 (Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE=1 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.6e-212
Identity = 380/559 (67.98%), Postives = 444/559 (79.43%), Query Frame = 0

Query: 33  MSASSE-TPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQ---EAVAEAAISSLNVE 92
           MS+ SE  P+    +A +E+ +  SKKAAKKEAAKLEKLRRRQ   EA    A  SL  E
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-E 60

Query: 93  DDPLSANYGDVPLSDLQSK---------EAKDVANWTQVGSLTGELKDKYVILRGRIQTI 152
           +D  S NYGDV L++LQS          EA +   WT V  L  E+ +  V++RGR+ T 
Sbjct: 61  NDEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTN 120

Query: 153 RAVAKKMAFLVVREKGFTVQCVVSEQPEL-VSRQMVKYVVGLSRESIVDVEGVVSVPNVA 212
           R  + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   
Sbjct: 121 RPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEP 180

Query: 213 IKGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNY 272
           + G TQQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNY
Sbjct: 181 LTGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNY 240

Query: 273 RVLDMRTPANQGIFRIQCQI---FRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQ 332
           RVLD+RTPANQ IF++Q ++   FR+ L  + FV IHTPKL+ GSSEGG++VFRL+YKGQ
Sbjct: 241 RVLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQ 300

Query: 333 PACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEV 392
           PACLAQSPQLHKQMAICGD  RVFE+GPVFRAEDSFTHRHLCEF GLDVEMEI+ HYSE+
Sbjct: 301 PACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEI 360

Query: 393 MDIVDRLFVSMFDSMNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEI 452
           MD+VD LFV +F S+N+ CKKEL+A+ KQYPFEPLK+L  TLRLTFEEG+QMLK+AGVE+
Sbjct: 361 MDLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEV 420

Query: 453 DPLGDLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRG 512
           DPLGDLNTE+ERKLG+LVLEKY TEFYILHRYP +VRPFYTM C DNP YSNSFDVFIRG
Sbjct: 421 DPLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRG 480

Query: 513 EEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCG 572
           EEIISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC 
Sbjct: 481 EEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCA 540

Query: 573 LNNIRKTSLFPRDPQRIAP 575
           LNNIRKTSLFPRDPQR++P
Sbjct: 541 LNNIRKTSLFPRDPQRLSP 558

BLAST of Cp4.1LG06g03580 vs. ExPASy Swiss-Prot
Match: Q8H104 (Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g26870 PE=2 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.5e-197
Identity = 354/532 (66.54%), Postives = 427/532 (80.26%), Query Frame = 0

Query: 49  DEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNV--EDDPLSANYGDVPLSDLQS 108
           +E  + +SKK +KK AAKLEKL R+QE   EA  SSL++  ED+  S+NYGDV  ++LQS
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQER-EEATSSSLSLEEEDESCSSNYGDVTTNELQS 67

Query: 109 K-EAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVREKGFTVQCVVSEQ 168
             E K++   T V +L  E+    V +RGR+   R V  K+ F+++RE GFTVQCVV E 
Sbjct: 68  AVEGKEL---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE- 127

Query: 169 PELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPI 228
              V   M+K+V  LSRES+V++ GVVS P   + G TQQVEI VRK+YC+S+++P+LP+
Sbjct: 128 -TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPL 187

Query: 229 NIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQI---FRQF 288
            +EDAARSE++IEK+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQCQ+   FR++
Sbjct: 188 VVEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREY 247

Query: 289 LLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIG 348
           L S+GF+EIHTPKLI GSSEGG++VFRLDYKGQPACLAQSPQLHKQMAICGD  RVFE+G
Sbjct: 248 LQSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVG 307

Query: 349 PVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDSMNQNCKKELEAID 408
           PVFRAEDSFTHRHLCEF GLDVEMEI+ HYSE+MD+V  LF  +F  + + C KELE++ 
Sbjct: 308 PVFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVR 367

Query: 409 KQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGRLVLEKYGTEFY 468
           KQYPF+ LK+L  TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLG+LVLEKY TEFY
Sbjct: 368 KQYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFY 427

Query: 469 ILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDV 528
           +LHRYP +VRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDV
Sbjct: 428 MLHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDV 487

Query: 529 KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 575
           KTI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 KTISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of Cp4.1LG06g03580 vs. ExPASy Swiss-Prot
Match: P14868 (Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2)

HSP 1 Score: 481.5 bits (1238), Expect = 1.3e-134
Identity = 257/508 (50.59%), Postives = 345/508 (67.91%), Query Frame = 0

Query: 71  RRRQEAVAEAAISSLNVEDDPLSANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYV 130
           R+ QE   E     ++  +D     YG   +S +   + K      +V  LT +  D+ V
Sbjct: 8   RKSQEKPREI----MDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVRDLTIQKADEVV 67

Query: 131 ILRGRIQTIRAVAKKMAFLVVREKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEG 190
            +R R+ T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 191 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVR 250
           VV   N  I   TQQ VE+ V+K+Y IS A P LP+ ++DA R EAE E+     E    
Sbjct: 128 VVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 251 VNQDTRLNYRVLDMRTPANQGIFRIQ---CQIFRQFLLSEGFVEIHTPKLIGGSSEGGAS 310
           VNQDTRL+ RV+D+RT  +Q +FR+Q   C +FR+ L+++GFVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 311 VFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEM 370
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 371 EIKTHYSEVMDIVDRLFVSMFDSMNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQ 430
               HY EVM+ +    V +F  + +  + E++ ++KQ+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 431 MLKDAGVEIDPLGDLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYS 490
           ML++AGVE+    DL+T  E+ LG LV EKY T+FYIL +YPL+VRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 491 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGL 550
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 551 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 575
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of Cp4.1LG06g03580 vs. ExPASy Swiss-Prot
Match: Q5R9I5 (Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.7e-134
Identity = 256/508 (50.39%), Postives = 346/508 (68.11%), Query Frame = 0

Query: 71  RRRQEAVAEAAISSLNVEDDPLSANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYV 130
           R+ QE   E     ++  +D     YG   +S +   + K      +V  LT +  D+ V
Sbjct: 8   RKSQEKPREI----MDAAEDYAKERYG---ISSMIQSQEKSDRVLVRVRDLTIQKADEVV 67

Query: 131 ILRGRIQTIRAVAKKMAFLVVREKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEG 190
            +R R+ T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 191 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVR 250
           VV   N  I+  TQQ VE+ V+K+Y IS A P LP+ ++DA R EAE E+     E    
Sbjct: 128 VVRKVNQKIESCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 251 VNQDTRLNYRVLDMRTPANQGIFRIQ---CQIFRQFLLSEGFVEIHTPKLIGGSSEGGAS 310
           VNQDTRL+ RV+D+RT  +Q +FR+Q   C +FR+ L+++GFVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 311 VFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEM 370
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 371 EIKTHYSEVMDIVDRLFVSMFDSMNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQ 430
               HY EVM+ +    V +F  + +  + E++ ++KQ+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 431 MLKDAGVEIDPLGDLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYS 490
           ML++AGVE+    DL+T  E+ LG L+ EKY T+FYIL +YPL+VRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLIKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 491 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGL 550
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GID++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 551 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 575
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of Cp4.1LG06g03580 vs. ExPASy Swiss-Prot
Match: Q922B2 (Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2)

HSP 1 Score: 480.3 bits (1235), Expect = 3.0e-134
Identity = 252/477 (52.83%), Postives = 336/477 (70.44%), Query Frame = 0

Query: 102 SDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVREKGFTVQCV 161
           S +QS+E  D     +V  LT +  D  V +R R+ T RA  K+  FLV+R++ F VQ +
Sbjct: 33  SMIQSQEKPDRV-LVRVKDLTVQKADDVVWVRARVHTSRAKGKQ-CFLVLRQQQFNVQAL 92

Query: 162 VSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQ-VEIQVRKVYCISKAM 221
           V+   +  S+QMVK+   +++ESI+DVEGVV   N  I   TQQ VE+ V+K+Y IS A 
Sbjct: 93  VA-VGDHASKQMVKFAANINKESIIDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAE 152

Query: 222 PSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQ---CQ 281
           P LP+ ++DA R E E E+  +A      VNQDTRL+ RV+D+RT  +Q IFR+Q   C 
Sbjct: 153 PRLPLQLDDAIRPEVEGEEDGRA-----TVNQDTRLDNRVIDLRTSTSQAIFRLQSGICH 212

Query: 282 IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGR 341
           +FR+ L+++GFVEI TPK+I  +SEGGA+VF + Y    A LAQSPQL+KQM IC DF +
Sbjct: 213 LFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEK 272

Query: 342 VFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDSMNQNCKKE 401
           VF IGPVFRAEDS THRHL EF GLD+EM    HY EV++ +    V +F  + +  + E
Sbjct: 273 VFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTE 332

Query: 402 LEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGRLVLEKY 461
           ++ + KQ+P EP K+L  TLRL + E + ML++AGVE+D   DL+T  E+ LGRLV EKY
Sbjct: 333 IQTVSKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMDDEEDLSTPNEKLLGRLVKEKY 392

Query: 462 GTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQA 521
            T+FY+L +YPL+VRPFYTMP   NP  SNS+D+F+RGEEI+SGAQR+H P+ L ERA  
Sbjct: 393 DTDFYVLDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALH 452

Query: 522 CGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 575
            GID++ I+ YIDSFR+GAPPH G G+GLERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 453 HGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of Cp4.1LG06g03580 vs. NCBI nr
Match: XP_023535979.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 542/545 (99.45%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. NCBI nr
Match: KAG6591612.1 (Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 539/545 (98.90%), Postives = 541/545 (99.27%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEA AEAAISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELK+KYVILRGRIQTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKEKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. NCBI nr
Match: XP_022936995.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 539/545 (98.90%), Postives = 540/545 (99.08%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEA AEAAISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FL ERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. NCBI nr
Match: XP_022976148.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 538/545 (98.72%), Postives = 539/545 (98.90%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETPIDE LSAGDEASKAVSKKAAKKEAAKLEKLRRRQEA AEAAISSLNVEDDPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHY EVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. NCBI nr
Match: XP_008466345.1 (PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Cucumis melo] >KAA0038775.1 aspartate--tRNA ligase 2 [Cucumis melo var. makuwa] >TYK31390.1 aspartate--tRNA ligase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 514/545 (94.31%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEA AE+AISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQI---FRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ+   FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           AICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. ExPASy TrEMBL
Match: A0A6J1FFA5 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1)

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 539/545 (98.90%), Postives = 540/545 (99.08%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEA AEAAISSLNVEDDPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FL ERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. ExPASy TrEMBL
Match: A0A6J1ILA3 (Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1)

HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 538/545 (98.72%), Postives = 539/545 (98.90%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETPIDE LSAGDEASKAVSKKAAKKEAAKLEKLRRRQEA AEAAISSLNVEDDPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHY EVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. ExPASy TrEMBL
Match: A0A5A7TBL8 (Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006980 PE=3 SV=1)

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 514/545 (94.31%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEA AE+AISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQI---FRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ+   FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           AICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. ExPASy TrEMBL
Match: A0A1S3CR09 (Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1)

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 514/545 (94.31%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASS+TPIDEPLSAGDE+SKAVSKKAAKKEAAKLEKLRRRQEA AE+AISSLNVEDDPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLSDLQSKE K + NWTQVGSLT ELKDKYVILRGRIQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 273 RIQCQI---FRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ+   FRQFLL E FVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           AICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFV+MFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           +N+ CKK LEA+DKQYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of Cp4.1LG06g03580 vs. ExPASy TrEMBL
Match: A0A6J1FKT3 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111446655 PE=3 SV=1)

HSP 1 Score: 1003 bits (2593), Expect = 0.0
Identity = 513/545 (94.13%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 33  MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 92
           MSASSETP+DE LSAG      VSKKAAKKEAAK EKLRRRQEA AE AISSL+VEDDPL
Sbjct: 1   MSASSETPMDESLSAG------VSKKAAKKEAAKQEKLRRRQEAAAEVAISSLDVEDDPL 60

Query: 93  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 152
           SANYGDVPLS+LQSKEAK++ NWTQVGSLTGELKDKYV+LRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSNLQSKEAKNIENWTQVGSLTGELKDKYVLLRGRVQTIRAVAKKMAFLVVR 120

Query: 153 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 212
           EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 213 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 272
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240

Query: 273 RIQCQ---IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 332
           RIQCQ   IFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGAIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 333 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 392
           AICGDFGRVFEIGPVFRAEDS+THRHLCEFTGLDVEMEIKTHYSEVM+IVDRLFV MFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMNIVDRLFVKMFDS 360

Query: 393 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 452
           +N+NCKKELEAID+QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNENCKKELEAIDRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 453 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 512
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 513 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 572
           FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 539

Query: 573 QRIAP 574
           QRIAP
Sbjct: 541 QRIAP 539

BLAST of Cp4.1LG06g03580 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.1e-213
Identity = 380/559 (67.98%), Postives = 444/559 (79.43%), Query Frame = 0

Query: 33  MSASSE-TPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQ---EAVAEAAISSLNVE 92
           MS+ SE  P+    +A +E+ +  SKKAAKKEAAKLEKLRRRQ   EA    A  SL  E
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-E 60

Query: 93  DDPLSANYGDVPLSDLQSK---------EAKDVANWTQVGSLTGELKDKYVILRGRIQTI 152
           +D  S NYGDV L++LQS          EA +   WT V  L  E+ +  V++RGR+ T 
Sbjct: 61  NDEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTN 120

Query: 153 RAVAKKMAFLVVREKGFTVQCVVSEQPEL-VSRQMVKYVVGLSRESIVDVEGVVSVPNVA 212
           R  + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   
Sbjct: 121 RPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEP 180

Query: 213 IKGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNY 272
           + G TQQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNY
Sbjct: 181 LTGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNY 240

Query: 273 RVLDMRTPANQGIFRIQCQI---FRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQ 332
           RVLD+RTPANQ IF++Q ++   FR+ L  + FV IHTPKL+ GSSEGG++VFRL+YKGQ
Sbjct: 241 RVLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQ 300

Query: 333 PACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEV 392
           PACLAQSPQLHKQMAICGD  RVFE+GPVFRAEDSFTHRHLCEF GLDVEMEI+ HYSE+
Sbjct: 301 PACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEI 360

Query: 393 MDIVDRLFVSMFDSMNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEI 452
           MD+VD LFV +F S+N+ CKKEL+A+ KQYPFEPLK+L  TLRLTFEEG+QMLK+AGVE+
Sbjct: 361 MDLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEV 420

Query: 453 DPLGDLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRG 512
           DPLGDLNTE+ERKLG+LVLEKY TEFYILHRYP +VRPFYTM C DNP YSNSFDVFIRG
Sbjct: 421 DPLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRG 480

Query: 513 EEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCG 572
           EEIISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC 
Sbjct: 481 EEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCA 540

Query: 573 LNNIRKTSLFPRDPQRIAP 575
           LNNIRKTSLFPRDPQR++P
Sbjct: 541 LNNIRKTSLFPRDPQRLSP 558

BLAST of Cp4.1LG06g03580 vs. TAIR 10
Match: AT4G31180.2 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.1e-213
Identity = 380/559 (67.98%), Postives = 444/559 (79.43%), Query Frame = 0

Query: 33  MSASSE-TPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQ---EAVAEAAISSLNVE 92
           MS+ SE  P+    +A +E+ +  SKKAAKKEAAKLEKLRRRQ   EA    A  SL  E
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLE-E 60

Query: 93  DDPLSANYGDVPLSDLQSK---------EAKDVANWTQVGSLTGELKDKYVILRGRIQTI 152
           +D  S NYGDV L++LQS          EA +   WT V  L  E+ +  V++RGR+ T 
Sbjct: 61  NDEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTN 120

Query: 153 RAVAKKMAFLVVREKGFTVQCVVSEQPEL-VSRQMVKYVVGLSRESIVDVEGVVSVPNVA 212
           R  + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   
Sbjct: 121 RPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEP 180

Query: 213 IKGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNY 272
           + G TQQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNY
Sbjct: 181 LTGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNY 240

Query: 273 RVLDMRTPANQGIFRIQCQI---FRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQ 332
           RVLD+RTPANQ IF++Q ++   FR+ L  + FV IHTPKL+ GSSEGG++VFRL+YKGQ
Sbjct: 241 RVLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQ 300

Query: 333 PACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEV 392
           PACLAQSPQLHKQMAICGD  RVFE+GPVFRAEDSFTHRHLCEF GLDVEMEI+ HYSE+
Sbjct: 301 PACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEI 360

Query: 393 MDIVDRLFVSMFDSMNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEI 452
           MD+VD LFV +F S+N+ CKKEL+A+ KQYPFEPLK+L  TLRLTFEEG+QMLK+AGVE+
Sbjct: 361 MDLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEV 420

Query: 453 DPLGDLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRG 512
           DPLGDLNTE+ERKLG+LVLEKY TEFYILHRYP +VRPFYTM C DNP YSNSFDVFIRG
Sbjct: 421 DPLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRG 480

Query: 513 EEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCG 572
           EEIISGAQRVH+PE L +RA  CGIDVKTI TYIDSFRYGAP HGGFGVGLERVVMLFC 
Sbjct: 481 EEIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCA 540

Query: 573 LNNIRKTSLFPRDPQRIAP 575
           LNNIRKTSLFPRDPQR++P
Sbjct: 541 LNNIRKTSLFPRDPQRLSP 558

BLAST of Cp4.1LG06g03580 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 690.6 bits (1781), Expect = 1.0e-198
Identity = 354/532 (66.54%), Postives = 427/532 (80.26%), Query Frame = 0

Query: 49  DEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNV--EDDPLSANYGDVPLSDLQS 108
           +E  + +SKK +KK AAKLEKL R+QE   EA  SSL++  ED+  S+NYGDV  ++LQS
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQER-EEATSSSLSLEEEDESCSSNYGDVTTNELQS 67

Query: 109 K-EAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVREKGFTVQCVVSEQ 168
             E K++   T V +L  E+    V +RGR+   R V  K+ F+++RE GFTVQCVV E 
Sbjct: 68  AVEGKEL---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE- 127

Query: 169 PELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPI 228
              V   M+K+V  LSRES+V++ GVVS P   + G TQQVEI VRK+YC+S+++P+LP+
Sbjct: 128 -TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPL 187

Query: 229 NIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQCQI---FRQF 288
            +EDAARSE++IEK+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQCQ+   FR++
Sbjct: 188 VVEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREY 247

Query: 289 LLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIG 348
           L S+GF+EIHTPKLI GSSEGG++VFRLDYKGQPACLAQSPQLHKQMAICGD  RVFE+G
Sbjct: 248 LQSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVG 307

Query: 349 PVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDSMNQNCKKELEAID 408
           PVFRAEDSFTHRHLCEF GLDVEMEI+ HYSE+MD+V  LF  +F  + + C KELE++ 
Sbjct: 308 PVFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVR 367

Query: 409 KQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGRLVLEKYGTEFY 468
           KQYPF+ LK+L  TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLG+LVLEKY TEFY
Sbjct: 368 KQYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFY 427

Query: 469 ILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIDV 528
           +LHRYP +VRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGIDV
Sbjct: 428 MLHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDV 487

Query: 529 KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 575
           KTI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 KTISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of Cp4.1LG06g03580 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 8.6e-28
Identity = 121/483 (25.05%), Postives = 201/483 (41.61%), Query Frame = 0

Query: 132 LRGRIQTIRAVAKKMAFLVVREKG--FTVQCVVSEQPELVSR-QMVKYVVGLSRESIVDV 191
           + G ++T+R+    + F+ + +      +QCV++   E   + +    + G S    V V
Sbjct: 114 IMGWVRTLRS-QSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGAS----VSV 173

Query: 192 EGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLV 251
           +G +    VA +G  Q+VE++V K+  + +   S PI                    Q  
Sbjct: 174 QGTI----VASQGTKQKVELKVEKIIVVGECDSSYPI--------------------QKK 233

Query: 252 RVNQDTRLNYRVLDMRTPANQGIFRIQ---CQIFRQFLLSEGFVEIHTPKLIGGSSEGGA 311
           RV+++       L  RT     + R++        +F    GFV + +P +     EG  
Sbjct: 234 RVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIITASDCEGAG 293

Query: 312 SVFRL------------------------------DYKGQPACLAQSPQLHKQMAICGDF 371
             F +                              D+ G+PA L  S QL+ +       
Sbjct: 294 EQFCVTTLIPSSHENTDTSIDAIPKTKGGLIDWSQDFFGKPAFLTVSGQLNGE-TYATAL 353

Query: 372 GRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDSMNQNCK 431
             V+  GP FRAE+S T RHL EF  ++ E+       + M         +   +  NCK
Sbjct: 354 SDVYTFGPTFRAENSNTSRHLAEFWMIEPELAF-ADLDDDMACATAYLQYVVKYVLDNCK 413

Query: 432 KELEAIDKQYPFEPLKYL-----RNTLRLTFEEGIQMLKDAGVEID-PLG---DLNTEAE 491
           +++E  D       ++ L     +  L+L + + I++L  A  + D P+    DL +E E
Sbjct: 414 EDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKFDFPVKWGLDLQSEHE 473

Query: 492 RKLGRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVH 551
           R +     E +G    I+  YP  ++ FY     D  T +    +  R  E+I G+QR  
Sbjct: 474 RYITE---EAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREE 533

Query: 552 VPEFLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFP 570
             E L  R     ++ ++   Y+D  RYG+ PH GFG+G ER+V    G++NIR    FP
Sbjct: 534 RLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFP 562

BLAST of Cp4.1LG06g03580 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 8.6e-28
Identity = 85/272 (31.25%), Postives = 141/272 (51.84%), Query Frame = 0

Query: 311 DYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKT 370
           D+ G+ A L  S QL  +   C     V+  GP FRAE+S T RHL EF  ++ E+    
Sbjct: 303 DFFGRQAFLTVSGQLQVETYACA-LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAF-A 362

Query: 371 HYSEVMDIVDRLFVSMFDSMNQNCKKELEAIDKQYP---FEPLKYLRNTL--RLTFEEGI 430
              + M+  +     M + + + C  ++E + K +     + LK + +T   R+T+ + I
Sbjct: 363 DLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAI 422

Query: 431 QMLKDA---GVEID---PLG-DLNTEAERKLGRLVLEKYGTEFYILHRYPLSVRPFYTMP 490
           ++L++A   G E D     G DL +E ER L  ++ +K      I++ YP  ++ FY M 
Sbjct: 423 ELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKP----LIVYNYPKGIKAFY-MR 482

Query: 491 CHDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQACGIDVKTIETYIDSFRYGAP 550
            +D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RYG  
Sbjct: 483 LNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTV 542

Query: 551 PHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 570
            H GFG+G ER+++   GL+NIR    FPR P
Sbjct: 543 KHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0841.6e-21267.98Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE... [more]
Q8H1041.5e-19766.54Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g268... [more]
P148681.3e-13450.59Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2[more]
Q5R9I51.7e-13450.39Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1[more]
Q922B23.0e-13452.83Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023535979.10.099.45aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
KAG6591612.10.098.90Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022936995.10.098.90aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata][more]
XP_022976148.10.098.72aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima][more]
XP_008466345.10.094.31PREDICTED: aspartate--tRNA ligase 2, cytoplasmic [Cucumis melo] >KAA0038775.1 as... [more]
Match NameE-valueIdentityDescription
A0A6J1FFA50.098.90Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1[more]
A0A6J1ILA30.098.72Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1[more]
A0A5A7TBL80.094.31Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CR090.094.31Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1[more]
A0A6J1FKT30.094.13Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111446655 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31180.11.1e-21367.98Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G31180.21.1e-21367.98Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G26870.11.0e-19866.54Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.18.6e-2825.05Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT5G56680.18.6e-2831.25Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 57..77
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 224..574
e-value: 3.8E-128
score: 429.5
NoneNo IPR availableGENE3D2.40.50.140coord: 88..222
e-value: 3.9E-38
score: 132.0
NoneNo IPR availablePANTHERPTHR43450:SF1ASPARTATE--TRNA LIGASE, CYTOPLASMICcoord: 48..574
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 249..570
e-value: 2.6896E-165
score: 471.28
NoneNo IPR availableCDDcd04320AspRS_cyto_Ncoord: 129..229
e-value: 3.79968E-43
score: 147.71
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 247..573
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 319..331
score: 55.24
coord: 489..505
score: 40.11
coord: 529..543
score: 55.15
coord: 336..349
score: 51.79
IPR004523Aspartate-tRNA synthetase, type 2TIGRFAMTIGR00458TIGR00458coord: 124..574
e-value: 8.8E-139
score: 461.1
IPR004523Aspartate-tRNA synthetase, type 2PANTHERPTHR43450ASPARTYL-TRNA SYNTHETASEcoord: 48..574
IPR004523Aspartate-tRNA synthetase, type 2HAMAPMF_02075Asp_tRNA_synth_type2coord: 113..574
score: 39.2318
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 252..568
e-value: 1.0E-74
score: 251.5
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 277..566
score: 19.57839
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 94..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g03580.1Cp4.1LG06g03580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006422 aspartyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
cellular_component GO:0005829 cytosol
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004815 aspartate-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding