Cp4.1LG06g03010 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g03010
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWRKY transcription factor
LocationCp4.1LG06: 1636222 .. 1638151 (-)
RNA-Seq ExpressionCp4.1LG06g03010
SyntenyCp4.1LG06g03010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTGAAGCAGAAAAAACACAAGTTTGAAGAAGAAATGTATTGGGAGAACGAACATCTCTCATTGAGCTTGGCAAACAAGGTGAACCCCATGACCCATTACCTTAATTTTGTGTTTTTTTTTTTAACCACCGAGCTCGTGGTTCAAATCACTCCACAAATGGTTCTCGATTTGAGCGAACCCCATGAGCCATTGAGCTTAGTTTATTGGGTATATCTTATTTTTTTAAGCTCGTGGTTCAAATGACTGTATGAATGATTTCCAATCTGAGCATAGATCAACTGATTGATGTAGTGTAGTATAGCAAAGTTCAAATTTTGAAGATGTTAGGGTTTCGTGTAAGGGTACGTGAATAACCCGAGTAGTTTTGTGGATTGTGAAGGCGCAGGGAAGTGATTCGAGTCGGAGATTGGAAGAAGAGTTGGATAGGAAAATGGTGGAGAATGGGAAGCTAAGTCAGATGTTGAGAATAATGTATGAGAAATACATTAATCTTCAGAAGCAAGTGATGTGTTTGGTGAGCCATCAAAAGCAGAGCTCAGAAATGGAAGCTCTAATTTCTTCAAGGAAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGGATTTGCAGCACAAGAGATGAAGATTTCAACAGGTGGCTTAAGAGGCCAAGACTCAATGGAAACCCTAAGGTTTCTAAGGTTTTTGTGCACAAAGATGCATCTGATCCAAGCTTGGTAAGTTTCTTAGCCCTACAAAACCCTAGCTAGCTAGGTGTTAGGCGTAACTAATTAGGGTTTCGGTTCGGGTTCGATAACCCGAGACTCATATCTGAATACTAAAAGAGAGAGAGACTCGCCTTGCGTATTGAATTGAGTACAGGGTTGATGCTTCTGACCGAACCACGGTTTGTTTCACCCAACTTGGTTTTGTTGTTCCCTTTCATAAATTTGTCCATAAGTTGACTTTTTAAAGCTCGGATTTAGCCGTTTAATTTGTTGAATTTGGGTAGGAAGGCCTATAAAGATTGACACTGAATTCAATATGAACATAGCTCAACTATAATTGACATATACTTCATTTTATGAGGTCAGAGATTCAAATCCCTGTTCCCGTTCAATGATTAAAATTAATTTTGGAGTCGTGATTTGGACAAGAGGGTTTGATTTAGAAACCCTAATTGGTATGAATTTTTTTATGAAAAAGGTGGTGAAAGATGGGTATCAATGGAGGAAGTATGGCCAAAAGGTCACAAGAGACAATCCTTCTCCAAGAGCTTACTTCAAGTGTTCCTCTGCACCAAATTGTCCAGTAAAAAAGAAGGTAAATTTTGTTTATAATTTTGAGAACAGTAAATAACGAAGTAAAAAATGGAAAATAATGTTTTAAAAATTACTGGTTTTTTTTTTTTTTTTTTTTACTGTGTTTAGGTGCAAAGAAGTTTGGAAGATCCAGCAATTTTGGTGGCCACTTACGAAGGAGAACACAACCATGCCAGCCATTTTCAAACTGAGCTTTCTTTAAGGTCCATCAATGGCGGGAAAGGCAGCGCAGTCCCGGTCTTAGCCACGATCAAGCCGTCGTGCGCCACCGTGACCCTCGACTTGATTCATGAAGATGGGCTGTTTAAGAGTCCTAAGGATTACGCGTCGTCGGAGTTGGCGGTGTCGACGGAGGCAGCGGTTTGGCAGGAGTTTTTAGTACAACAAATGGCTTCTTCTTTGAAGAAGGATCCTGAATTTGCTGGGATTGTTGCTGGTGCCATTTCCGACAAGGTTTTGGGAAACCAAACAAACTGAGAATAAACCTTAAAACCATCAAATGCTAAAGGCTTTGTGTTCTATTTTTGCTTTCTTTTGTGAATATTTGTTTGTTTGGATTATGTGTTGATTATCAATTTTGTTTTGTCAAAAAATAAATTAACAAGCCATTTTATTAAT

mRNA sequence

GTTTTGAAGCAGAAAAAACACAAGTTTGAAGAAGAAATGTATTGGGAGAACGAACATCTCTCATTGAGCTTGGCAAACAAGGCGCAGGGAAGTGATTCGAGTCGGAGATTGGAAGAAGAGTTGGATAGGAAAATGGTGGAGAATGGGAAGCTAAGTCAGATGTTGAGAATAATGTATGAGAAATACATTAATCTTCAGAAGCAAGTGATGTGTTTGGTGAGCCATCAAAAGCAGAGCTCAGAAATGGAAGCTCTAATTTCTTCAAGGAAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGGATTTGCAGCACAAGAGATGAAGATTTCAACAGGTGGCTTAAGAGGCCAAGACTCAATGGAAACCCTAAGGTTTCTAAGGTTTTTGTGCACAAAGATGCATCTGATCCAAGCTTGGTGGTGAAAGATGGGTATCAATGGAGGAAGTATGGCCAAAAGGTCACAAGAGACAATCCTTCTCCAAGAGCTTACTTCAAGTGTTCCTCTGCACCAAATTGTCCAGTAAAAAAGAAGGTGCAAAGAAGTTTGGAAGATCCAGCAATTTTGGTGGCCACTTACGAAGGAGAACACAACCATGCCAGCCATTTTCAAACTGAGCTTTCTTTAAGGTCCATCAATGGCGGGAAAGGCAGCGCAGTCCCGGTCTTAGCCACGATCAAGCCGTCGTGCGCCACCGTGACCCTCGACTTGATTCATGAAGATGGGCTGTTTAAGAGTCCTAAGGATTACGCGTCGTCGGAGTTGGCGGTGTCGACGGAGGCAGCGGTTTGGCAGGAGTTTTTAGTACAACAAATGGCTTCTTCTTTGAAGAAGGATCCTGAATTTGCTGGGATTGTTGCTGGTGCCATTTCCGACAAGGTTTTGGGAAACCAAACAAACTGAGAATAAACCTTAAAACCATCAAATGCTAAAGGCTTTGTGTTCTATTTTTGCTTTCTTTTGTGAATATTTGTTTGTTTGGATTATGTGTTGATTATCAATTTTGTTTTGTCAAAAAATAAATTAACAAGCCATTTTATTAAT

Coding sequence (CDS)

GTTTTGAAGCAGAAAAAACACAAGTTTGAAGAAGAAATGTATTGGGAGAACGAACATCTCTCATTGAGCTTGGCAAACAAGGCGCAGGGAAGTGATTCGAGTCGGAGATTGGAAGAAGAGTTGGATAGGAAAATGGTGGAGAATGGGAAGCTAAGTCAGATGTTGAGAATAATGTATGAGAAATACATTAATCTTCAGAAGCAAGTGATGTGTTTGGTGAGCCATCAAAAGCAGAGCTCAGAAATGGAAGCTCTAATTTCTTCAAGGAAGAGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGGATTTGCAGCACAAGAGATGAAGATTTCAACAGGTGGCTTAAGAGGCCAAGACTCAATGGAAACCCTAAGGTTTCTAAGGTTTTTGTGCACAAAGATGCATCTGATCCAAGCTTGGTGGTGAAAGATGGGTATCAATGGAGGAAGTATGGCCAAAAGGTCACAAGAGACAATCCTTCTCCAAGAGCTTACTTCAAGTGTTCCTCTGCACCAAATTGTCCAGTAAAAAAGAAGGTGCAAAGAAGTTTGGAAGATCCAGCAATTTTGGTGGCCACTTACGAAGGAGAACACAACCATGCCAGCCATTTTCAAACTGAGCTTTCTTTAAGGTCCATCAATGGCGGGAAAGGCAGCGCAGTCCCGGTCTTAGCCACGATCAAGCCGTCGTGCGCCACCGTGACCCTCGACTTGATTCATGAAGATGGGCTGTTTAAGAGTCCTAAGGATTACGCGTCGTCGGAGTTGGCGGTGTCGACGGAGGCAGCGGTTTGGCAGGAGTTTTTAGTACAACAAATGGCTTCTTCTTTGAAGAAGGATCCTGAATTTGCTGGGATTGTTGCTGGTGCCATTTCCGACAAGGTTTTGGGAAACCAAACAAACTGA

Protein sequence

VLKQKKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHEDGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN
Homology
BLAST of Cp4.1LG06g03010 vs. ExPASy Swiss-Prot
Match: Q9SAH7 (Probable WRKY transcription factor 40 OS=Arabidopsis thaliana OX=3702 GN=WRKY40 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 7.8e-41
Identity = 117/272 (43.01%), Postives = 160/272 (58.82%), Query Frame = 0

Query: 37  LEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQKQSSEMEALISSRKREEEEE 96
           L EEL+R   EN KLS+ML +M + Y  L+KQ+M  V ++   +E + +   +KR+    
Sbjct: 33  LVEELNRVSAENKKLSEMLTLMCDNYNVLRKQLMEYV-NKSNITERDQISPPKKRKSPAR 92

Query: 97  EEE---EEEEGICSTRDEDFNRWL---KRPRLNGNPKVSKVFVHKDASDPSLVVKDGYQW 156
           E+        G+  +   D + +L   +R       KVS+V+   +ASD +LVVKDGYQW
Sbjct: 93  EDAFSCAVIGGVSESSSTDQDEYLCKKQREETVVKEKVSRVYYKTEASDTTLVVKDGYQW 152

Query: 157 RKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYEGEHNHASHFQTEL 216
           RKYGQKVTRDNPSPRAYFKC+ AP+C VKKKVQRS+ED ++LVATYEGEHNH    Q + 
Sbjct: 153 RKYGQKVTRDNPSPRAYFKCACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDS 212

Query: 217 S---LRSINGGKGSAVPVLATIKPSCA--TVTLDLIHEDGLFKSPKDYASSELAVSTEAA 276
           +    R I+ G  ++ PV A  + S      T+D+I E     SP            +  
Sbjct: 213 NNGLNRHISHGGSASTPVAANRRSSLTVPVTTVDMI-ESKKVTSPTS--------RIDFP 272

Query: 277 VWQEFLVQQMASSLKKDPEFAGIVAGAISDKV 298
             Q+ LV+QMASSL KDP F   +A A++ K+
Sbjct: 273 QVQKLLVEQMASSLTKDPNFTAALAAAVTGKL 294

BLAST of Cp4.1LG06g03010 vs. ExPASy Swiss-Prot
Match: Q9C5T4 (WRKY transcription factor 18 OS=Arabidopsis thaliana OX=3702 GN=WRKY18 PE=1 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-39
Identity = 125/291 (42.96%), Postives = 161/291 (55.33%), Query Frame = 0

Query: 32  DSSRRLEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQKQSSEMEAL-ISSRK 91
           +S+  L EEL+R   EN KL++ML  + E Y  L   +  L S  +QS E+E   I  +K
Sbjct: 44  ESASELREELNRVNSENKKLTEMLARVCESYNELHNHLEKLQS--RQSPEIEQTDIPIKK 103

Query: 92  REEEEEE--------EEEEEEGICSTRD--------EDFNRWL--KRPRLN--GNPKVSK 151
           R+++ +E           + E   S  D        E  N+ L  KRP  +     KVS 
Sbjct: 104 RKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCKRPVTDSFNKAKVST 163

Query: 152 VFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPA 211
           V+V  + SD SL VKDG+QWRKYGQKVTRDNPSPRAYF+CS AP+CPVKKKVQRS EDP+
Sbjct: 164 VYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPS 223

Query: 212 ILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLI---HEDGLF 271
           +LVATYEG HNH             N  +G      AT +   +TVTLDL+   H   L 
Sbjct: 224 LLVATYEGTHNHLGP----------NASEGD-----ATSQGGSSTVTLDLVNGCHRLALE 283

Query: 272 KSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVL 299
           K+ +D               QE L+QQMASSL KD +F   +A AIS +++
Sbjct: 284 KNERD------------NTMQEVLIQQMASSLTKDSKFTAALAAAISGRLM 305

BLAST of Cp4.1LG06g03010 vs. ExPASy Swiss-Prot
Match: Q6IEK5 (WRKY transcription factor WRKY76 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY76 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.4e-36
Identity = 107/286 (37.41%), Postives = 153/286 (53.50%), Query Frame = 0

Query: 26  NKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYI--------NLQKQVMCLVSHQK 85
           +K+  +D ++ LE ++ +   EN +L++++  +Y   I            + +  +S +K
Sbjct: 48  SKSMTNDEAKILEAKVTQMSEENRRLTEVIARLYGGQIARLGLDGSASPPRPVSPLSGKK 107

Query: 86  QSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNGNPKVSKVFVHKDA 145
           +S E     +S        +  + +       D+   R +         KVS+V    D 
Sbjct: 108 RSRESMETANSCDANSNRHQGGDADHAESFAADDGTCRRI---------KVSRVCRRIDP 167

Query: 146 SDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYE 205
           SD SLVVKDGYQWRKYGQKVTRDNPSPRAYF+C+ AP+CPVKKKVQRS ED ++LVATYE
Sbjct: 168 SDTSLVVKDGYQWRKYGQKVTRDNPSPRAYFRCAFAPSCPVKKKVQRSAEDSSLLVATYE 227

Query: 206 GEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHEDGLFK------SPK 265
           GEHNH         L +  GG G ++P   +I  S  T+TLDL    G  +       P 
Sbjct: 228 GEHNHPHPSPRAGELPAAVGGAGGSLPCSISINSSGPTITLDLTKNGGAVQVVEAAHPPP 287

Query: 266 DYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKV 298
                E+     +  ++  LV+QMAS+L  DP+F G +A AI  K+
Sbjct: 288 PPDLKEVCREVASPEFRTALVEQMASALTSDPKFTGALAAAILQKL 324

BLAST of Cp4.1LG06g03010 vs. ExPASy Swiss-Prot
Match: Q6IEL0 (WRKY transcription factor WRKY71 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY71 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.4e-36
Identity = 113/296 (38.18%), Postives = 146/296 (49.32%), Query Frame = 0

Query: 37  LEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLV-----------SHQKQSSEMEAL 96
           LE EL R   EN +LS+ML  +  KY  LQ Q   +V           ++Q  +SE  ++
Sbjct: 53  LEAELKRMGAENRQLSEMLAAVAAKYEALQSQFSDMVTASANNGGGGGNNQSSTSEGGSV 112

Query: 97  ISSRKREEEEEEEE---------------------EEEEGICSTRDEDFNRWLKRPRLNG 156
             SRKR+ E  ++                          G     +       KR R   
Sbjct: 113 SPSRKRKSESLDDSPPPPPPPHPHAAPHHMHVMPGAAAAGYADQTECTSGEPCKRIREEC 172

Query: 157 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 216
            PK+SK++VH D SD SLVVKDGYQWRKYGQKVT+DNP PRAYF+CS AP CPVKKKVQR
Sbjct: 173 KPKISKLYVHADPSDLSLVVKDGYQWRKYGQKVTKDNPCPRAYFRCSFAPACPVKKKVQR 232

Query: 217 SLEDPAILVATYEGEHNHASHFQ--TELSLRSINGGKGSAVPVLATIKPSCATVTLDLIH 276
           S ED  ILVATYEGEHNH          +  S   GK +  P  A      A V     H
Sbjct: 233 SAEDNTILVATYEGEHNHGQPPPPLQSAAQNSDGSGKSAGKPPHAPAAAPPAPVVPHRQH 292

Query: 277 EDGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVL 299
           E  +    +  A++   +       +  L +QMA +L +DP F   +  A+S ++L
Sbjct: 293 EPVVVNGEQQAAAASEMI-------RRNLAEQMAMTLTRDPSFKAALVTALSGRIL 341

BLAST of Cp4.1LG06g03010 vs. ExPASy Swiss-Prot
Match: Q6EPZ2 (WRKY transcription factor WRKY76 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY76 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.8e-36
Identity = 106/286 (37.06%), Postives = 153/286 (53.50%), Query Frame = 0

Query: 26  NKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYI--------NLQKQVMCLVSHQK 85
           +K+  +D ++ +E ++ +   EN +L++++  +Y   I            + +  +S +K
Sbjct: 48  SKSMTNDEAKIVEAKVTQMSEENRRLTEVIARLYGGQIPRLGLDGSASPPRPVSPLSGKK 107

Query: 86  QSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNGNPKVSKVFVHKDA 145
           +S E     +S        +  + +       D+   R +         KVS+V    D 
Sbjct: 108 RSRESMETANSCDANSNRHQGGDADHAESFAADDGTCRRI---------KVSRVCRRIDP 167

Query: 146 SDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYE 205
           SD SLVVKDGYQWRKYGQKVTRDNPSPRAYF+C+ AP+CPVKKKVQRS ED ++LVATYE
Sbjct: 168 SDTSLVVKDGYQWRKYGQKVTRDNPSPRAYFRCAFAPSCPVKKKVQRSAEDSSLLVATYE 227

Query: 206 GEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHEDGLFK------SPK 265
           GEHNH         L +  GG G ++P   +I  S  T+TLDL    G  +       P 
Sbjct: 228 GEHNHPHPSPRAGELPAAAGGAGGSLPCSISINSSGPTITLDLTKNGGAVQVVEAAHPPP 287

Query: 266 DYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKV 298
                E+     +  ++  LV+QMAS+L  DP+F G +A AI  K+
Sbjct: 288 PPDLKEVCREVASPEFRTALVEQMASALTSDPKFTGALAAAILQKL 324

BLAST of Cp4.1LG06g03010 vs. NCBI nr
Match: XP_023535967.1 (probable WRKY transcription factor 40 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579 bits (1493), Expect = 1.09e-207
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 28  KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 87

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNG 124
           LQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNG
Sbjct: 88  LQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNG 147

Query: 125 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 184
           NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR
Sbjct: 148 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 207

Query: 185 SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 244
           SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED
Sbjct: 208 SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 267

Query: 245 GLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN 303
           GLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN
Sbjct: 268 GLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN 326

BLAST of Cp4.1LG06g03010 vs. NCBI nr
Match: KAG7024421.1 (putative WRKY transcription factor 40 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 566 bits (1458), Expect = 2.63e-202
Identity = 293/298 (98.32%), Postives = 294/298 (98.66%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KKHKFEEEMYWE EHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 28  KKHKFEEEMYWEKEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 87

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNG 124
           LQKQV+CLVSHQKQSSEMEALISSRKRE E EEEEEEEEGICSTRDEDFNRWLKRPRLNG
Sbjct: 88  LQKQVVCLVSHQKQSSEMEALISSRKREAEGEEEEEEEEGICSTRDEDFNRWLKRPRLNG 147

Query: 125 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 184
           NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR
Sbjct: 148 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 207

Query: 185 SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 244
           SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED
Sbjct: 208 SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 267

Query: 245 GLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT 302
           GLFKSPKDYASSE AVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT
Sbjct: 268 GLFKSPKDYASSESAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT 325

BLAST of Cp4.1LG06g03010 vs. NCBI nr
Match: KAG6591536.1 (putative WRKY transcription factor 40, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564 bits (1453), Expect = 1.26e-201
Identity = 294/301 (97.67%), Postives = 295/301 (98.01%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KKHKFEEEMYWE EHLSLSLANK QGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 20  KKHKFEEEMYWEKEHLSLSLANKGQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 79

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKRE---EEEEEEEEEEEGICSTRDEDFNRWLKRPR 124
           LQKQV+CLVSHQKQSSEMEALISSRKRE   EEEEEEEEEEEGICSTRDEDFNRWLKRPR
Sbjct: 80  LQKQVVCLVSHQKQSSEMEALISSRKREAEGEEEEEEEEEEEGICSTRDEDFNRWLKRPR 139

Query: 125 LNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKK 184
           LNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKK
Sbjct: 140 LNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKK 199

Query: 185 VQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLI 244
           VQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLI
Sbjct: 200 VQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLI 259

Query: 245 HEDGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQ 302
           HEDGLFKSPKDYASSE AVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQ
Sbjct: 260 HEDGLFKSPKDYASSESAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQ 319

BLAST of Cp4.1LG06g03010 vs. NCBI nr
Match: XP_022975893.1 (probable WRKY transcription factor 40 [Cucurbita maxima])

HSP 1 Score: 562 bits (1448), Expect = 9.45e-201
Identity = 294/304 (96.71%), Postives = 295/304 (97.04%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KK KFEEEMYW+ EHLSLSLANKA GSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 28  KKDKFEEEMYWDKEHLSLSLANKAHGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 87

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKR-----EEEEEEEEEEEEGICSTRDEDFNRWLKR 124
           LQKQVMCLVSHQKQSSEMEALISSRKR     EEEEEEEEEEEEGICSTRDEDFNRWLKR
Sbjct: 88  LQKQVMCLVSHQKQSSEMEALISSRKRKAEGGEEEEEEEEEEEEGICSTRDEDFNRWLKR 147

Query: 125 PRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVK 184
           PRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVK
Sbjct: 148 PRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVK 207

Query: 185 KKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLD 244
           KKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLD
Sbjct: 208 KKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLD 267

Query: 245 LIHEDGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLG 303
           LIHEDGLFKSPKDYASSE AVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLG
Sbjct: 268 LIHEDGLFKSPKDYASSESAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLG 327

BLAST of Cp4.1LG06g03010 vs. NCBI nr
Match: XP_022936945.1 (probable WRKY transcription factor 40 [Cucurbita moschata])

HSP 1 Score: 554 bits (1428), Expect = 9.10e-198
Identity = 289/298 (96.98%), Postives = 291/298 (97.65%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KKHKFEEEMYWE EHLSLSLANK QGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 28  KKHKFEEEMYWEKEHLSLSLANKGQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 87

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNG 124
           LQKQV+CLVSHQKQSSEMEALISSRKRE E EEEEEEEEGICSTRDEDFNRWLKRPRLNG
Sbjct: 88  LQKQVVCLVSHQKQSSEMEALISSRKREAEGEEEEEEEEGICSTRDEDFNRWLKRPRLNG 147

Query: 125 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 184
           NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKV  
Sbjct: 148 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKV-- 207

Query: 185 SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 244
           +LEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED
Sbjct: 208 NLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 267

Query: 245 GLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT 302
           GLFKSPKDYASSE AVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT
Sbjct: 268 GLFKSPKDYASSESAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT 323

BLAST of Cp4.1LG06g03010 vs. ExPASy TrEMBL
Match: A0A6J1IKJ7 (probable WRKY transcription factor 40 OS=Cucurbita maxima OX=3661 GN=LOC111476462 PE=4 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 4.58e-201
Identity = 294/304 (96.71%), Postives = 295/304 (97.04%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KK KFEEEMYW+ EHLSLSLANKA GSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 28  KKDKFEEEMYWDKEHLSLSLANKAHGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 87

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKR-----EEEEEEEEEEEEGICSTRDEDFNRWLKR 124
           LQKQVMCLVSHQKQSSEMEALISSRKR     EEEEEEEEEEEEGICSTRDEDFNRWLKR
Sbjct: 88  LQKQVMCLVSHQKQSSEMEALISSRKRKAEGGEEEEEEEEEEEEGICSTRDEDFNRWLKR 147

Query: 125 PRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVK 184
           PRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVK
Sbjct: 148 PRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVK 207

Query: 185 KKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLD 244
           KKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLD
Sbjct: 208 KKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLD 267

Query: 245 LIHEDGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLG 303
           LIHEDGLFKSPKDYASSE AVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLG
Sbjct: 268 LIHEDGLFKSPKDYASSESAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLG 327

BLAST of Cp4.1LG06g03010 vs. ExPASy TrEMBL
Match: A0A6J1FF55 (probable WRKY transcription factor 40 OS=Cucurbita moschata OX=3662 GN=LOC111443379 PE=4 SV=1)

HSP 1 Score: 554 bits (1428), Expect = 4.41e-198
Identity = 289/298 (96.98%), Postives = 291/298 (97.65%), Query Frame = 0

Query: 5   KKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 64
           KKHKFEEEMYWE EHLSLSLANK QGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN
Sbjct: 28  KKHKFEEEMYWEKEHLSLSLANKGQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYIN 87

Query: 65  LQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLNG 124
           LQKQV+CLVSHQKQSSEMEALISSRKRE E EEEEEEEEGICSTRDEDFNRWLKRPRLNG
Sbjct: 88  LQKQVVCLVSHQKQSSEMEALISSRKREAEGEEEEEEEEGICSTRDEDFNRWLKRPRLNG 147

Query: 125 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQR 184
           NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKV  
Sbjct: 148 NPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKV-- 207

Query: 185 SLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 244
           +LEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED
Sbjct: 208 NLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHED 267

Query: 245 GLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT 302
           GLFKSPKDYASSE AVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT
Sbjct: 268 GLFKSPKDYASSESAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQT 323

BLAST of Cp4.1LG06g03010 vs. ExPASy TrEMBL
Match: S0F0E6 (WRKY transcription factor OS=Cucumis sativus OX=3659 GN=WRKY32 PE=2 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 1.68e-167
Identity = 253/300 (84.33%), Postives = 273/300 (91.00%), Query Frame = 0

Query: 4   QKKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYI 63
           QK+ +F+ E+Y + E L+LSL+NK  GS+SS  LE+ELDRK+ ENGKLSQMLRIMYEKYI
Sbjct: 29  QKRDQFDGEIYGDKEKLALSLSNK--GSESSPTLEQELDRKIQENGKLSQMLRIMYEKYI 88

Query: 64  NLQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLN 123
           NLQKQVM L+S+QKQS+EME + S +++ E E+E+ E  EGICSTRDEDFNRWLKRPRLN
Sbjct: 89  NLQKQVMYLLSNQKQSTEMEGVCSRKRKAEGEQEDYENLEGICSTRDEDFNRWLKRPRLN 148

Query: 124 GNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ 183
           GN KVSKVFV KDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ
Sbjct: 149 GNSKVSKVFVQKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ 208

Query: 184 RSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE 243
           RSLEDP ILVATYEGEH+HASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE
Sbjct: 209 RSLEDPTILVATYEGEHSHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE 268

Query: 244 DGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN 303
           DGLFKSPKDYASSE   S EAAVWQEFLVQQMASSLKKDPEFAGIVAGAIS KVLGNQTN
Sbjct: 269 DGLFKSPKDYASSE---SAEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISGKVLGNQTN 323

BLAST of Cp4.1LG06g03010 vs. ExPASy TrEMBL
Match: A0A0A0L324 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652000 PE=4 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 1.74e-167
Identity = 253/300 (84.33%), Postives = 273/300 (91.00%), Query Frame = 0

Query: 4   QKKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYI 63
           QK+ +F+ E+Y + E L+LSL+NK  GS+SS  LE+ELDRK+ ENGKLSQMLRIMYEKYI
Sbjct: 30  QKRDQFDGEIYGDKEKLALSLSNK--GSESSPTLEQELDRKIQENGKLSQMLRIMYEKYI 89

Query: 64  NLQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLN 123
           NLQKQVM L+S+QKQS+EME + S +++ E E+E+ E  EGICSTRDEDFNRWLKRPRLN
Sbjct: 90  NLQKQVMYLLSNQKQSTEMEGVCSRKRKAEGEQEDYENLEGICSTRDEDFNRWLKRPRLN 149

Query: 124 GNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ 183
           GN KVSKVFV KDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ
Sbjct: 150 GNSKVSKVFVQKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ 209

Query: 184 RSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE 243
           RSLEDP ILVATYEGEH+HASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE
Sbjct: 210 RSLEDPTILVATYEGEHSHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE 269

Query: 244 DGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN 303
           DGLFKSPKDYASSE   S EAAVWQEFLVQQMASSLKKDPEFAGIVAGAIS KVLGNQTN
Sbjct: 270 DGLFKSPKDYASSE---SAEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISGKVLGNQTN 324

BLAST of Cp4.1LG06g03010 vs. ExPASy TrEMBL
Match: A0A5A7VC91 (Putative WRKY transcription factor 40 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003090 PE=4 SV=1)

HSP 1 Score: 476 bits (1224), Expect = 4.80e-167
Identity = 253/300 (84.33%), Postives = 272/300 (90.67%), Query Frame = 0

Query: 4   QKKHKFEEEMYWENEHLSLSLANKAQGSDSSRRLEEELDRKMVENGKLSQMLRIMYEKYI 63
           QK+ +F+ E+Y   E LSLSL+NK  GSDSS  LE+ELDRK+ ENGKLSQMLRIMYEKYI
Sbjct: 29  QKRDQFDGEIYGVQEKLSLSLSNK--GSDSSPTLEQELDRKIQENGKLSQMLRIMYEKYI 88

Query: 64  NLQKQVMCLVSHQKQSSEMEALISSRKREEEEEEEEEEEEGICSTRDEDFNRWLKRPRLN 123
           NLQKQVM L+S+QKQ++EME + S +++ E ++E+ E  EGICSTRDEDFNRWLKRPRLN
Sbjct: 89  NLQKQVMYLLSNQKQNTEMEGVCSRKRKAEGDQEDYENLEGICSTRDEDFNRWLKRPRLN 148

Query: 124 GNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ 183
           GN KVSKVFV KDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ
Sbjct: 149 GNSKVSKVFVQKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQ 208

Query: 184 RSLEDPAILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE 243
           RSLEDP ILVATYEGEH+HASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE
Sbjct: 209 RSLEDPTILVATYEGEHSHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLIHE 268

Query: 244 DGLFKSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVLGNQTN 303
           DGLFKSPKDYASSE   S EAAVWQEFLVQQMASSLKKDPEFAGIVAGAIS KVLGNQTN
Sbjct: 269 DGLFKSPKDYASSE---SAEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISGKVLGNQTN 323

BLAST of Cp4.1LG06g03010 vs. TAIR 10
Match: AT1G80840.1 (WRKY DNA-binding protein 40 )

HSP 1 Score: 169.1 bits (427), Expect = 5.5e-42
Identity = 117/272 (43.01%), Postives = 160/272 (58.82%), Query Frame = 0

Query: 37  LEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQKQSSEMEALISSRKREEEEE 96
           L EEL+R   EN KLS+ML +M + Y  L+KQ+M  V ++   +E + +   +KR+    
Sbjct: 33  LVEELNRVSAENKKLSEMLTLMCDNYNVLRKQLMEYV-NKSNITERDQISPPKKRKSPAR 92

Query: 97  EEE---EEEEGICSTRDEDFNRWL---KRPRLNGNPKVSKVFVHKDASDPSLVVKDGYQW 156
           E+        G+  +   D + +L   +R       KVS+V+   +ASD +LVVKDGYQW
Sbjct: 93  EDAFSCAVIGGVSESSSTDQDEYLCKKQREETVVKEKVSRVYYKTEASDTTLVVKDGYQW 152

Query: 157 RKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYEGEHNHASHFQTEL 216
           RKYGQKVTRDNPSPRAYFKC+ AP+C VKKKVQRS+ED ++LVATYEGEHNH    Q + 
Sbjct: 153 RKYGQKVTRDNPSPRAYFKCACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDS 212

Query: 217 S---LRSINGGKGSAVPVLATIKPSCA--TVTLDLIHEDGLFKSPKDYASSELAVSTEAA 276
           +    R I+ G  ++ PV A  + S      T+D+I E     SP            +  
Sbjct: 213 NNGLNRHISHGGSASTPVAANRRSSLTVPVTTVDMI-ESKKVTSPTS--------RIDFP 272

Query: 277 VWQEFLVQQMASSLKKDPEFAGIVAGAISDKV 298
             Q+ LV+QMASSL KDP F   +A A++ K+
Sbjct: 273 QVQKLLVEQMASSLTKDPNFTAALAAAVTGKL 294

BLAST of Cp4.1LG06g03010 vs. TAIR 10
Match: AT4G31800.1 (WRKY DNA-binding protein 18 )

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-40
Identity = 125/291 (42.96%), Postives = 161/291 (55.33%), Query Frame = 0

Query: 32  DSSRRLEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQKQSSEMEAL-ISSRK 91
           +S+  L EEL+R   EN KL++ML  + E Y  L   +  L S  +QS E+E   I  +K
Sbjct: 44  ESASELREELNRVNSENKKLTEMLARVCESYNELHNHLEKLQS--RQSPEIEQTDIPIKK 103

Query: 92  REEEEEE--------EEEEEEGICSTRD--------EDFNRWL--KRPRLN--GNPKVSK 151
           R+++ +E           + E   S  D        E  N+ L  KRP  +     KVS 
Sbjct: 104 RKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCKRPVTDSFNKAKVST 163

Query: 152 VFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPA 211
           V+V  + SD SL VKDG+QWRKYGQKVTRDNPSPRAYF+CS AP+CPVKKKVQRS EDP+
Sbjct: 164 VYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPS 223

Query: 212 ILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLI---HEDGLF 271
           +LVATYEG HNH             N  +G      AT +   +TVTLDL+   H   L 
Sbjct: 224 LLVATYEGTHNHLGP----------NASEGD-----ATSQGGSSTVTLDLVNGCHRLALE 283

Query: 272 KSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVL 299
           K+ +D               QE L+QQMASSL KD +F   +A AIS +++
Sbjct: 284 KNERD------------NTMQEVLIQQMASSLTKDSKFTAALAAAISGRLM 305

BLAST of Cp4.1LG06g03010 vs. TAIR 10
Match: AT4G31800.2 (WRKY DNA-binding protein 18 )

HSP 1 Score: 164.5 bits (415), Expect = 1.4e-40
Identity = 125/291 (42.96%), Postives = 161/291 (55.33%), Query Frame = 0

Query: 32  DSSRRLEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQKQSSEMEAL-ISSRK 91
           +S+  L EEL+R   EN KL++ML  + E Y  L   +  L S  +QS E+E   I  +K
Sbjct: 43  ESASELREELNRVNSENKKLTEMLARVCESYNELHNHLEKLQS--RQSPEIEQTDIPIKK 102

Query: 92  REEEEEE--------EEEEEEGICSTRD--------EDFNRWL--KRPRLN--GNPKVSK 151
           R+++ +E           + E   S  D        E  N+ L  KRP  +     KVS 
Sbjct: 103 RKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCKRPVTDSFNKAKVST 162

Query: 152 VFVHKDASDPSLVVKDGYQWRKYGQKVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPA 211
           V+V  + SD SL VKDG+QWRKYGQKVTRDNPSPRAYF+CS AP+CPVKKKVQRS EDP+
Sbjct: 163 VYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPS 222

Query: 212 ILVATYEGEHNHASHFQTELSLRSINGGKGSAVPVLATIKPSCATVTLDLI---HEDGLF 271
           +LVATYEG HNH             N  +G      AT +   +TVTLDL+   H   L 
Sbjct: 223 LLVATYEGTHNHLGP----------NASEGD-----ATSQGGSSTVTLDLVNGCHRLALE 282

Query: 272 KSPKDYASSELAVSTEAAVWQEFLVQQMASSLKKDPEFAGIVAGAISDKVL 299
           K+ +D               QE L+QQMASSL KD +F   +A AIS +++
Sbjct: 283 KNERD------------NTMQEVLIQQMASSLTKDSKFTAALAAAISGRLM 304

BLAST of Cp4.1LG06g03010 vs. TAIR 10
Match: AT2G25000.1 (WRKY DNA-binding protein 60 )

HSP 1 Score: 143.7 bits (361), Expect = 2.5e-34
Identity = 107/263 (40.68%), Postives = 140/263 (53.23%), Query Frame = 0

Query: 37  LEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMCLVSHQK-QSSEMEALISSRKREEEE 96
           L++E++R   EN KL++ML  + EKY  L   +  L S +  +S   +    + KR++E 
Sbjct: 45  LQDEINRVNSENKKLTEMLARVCEKYYALNNLMEELQSRKSPESVNFQNKQLTGKRKQEL 104

Query: 97  EEEEEEEEGICSTRDEDFNRWLKRPRLNGNPKVSKVFVHKDASDPSLVVKDGYQWRKYGQ 156
           +E      G+     E+          N    VS  +   + SD SL VKDGYQWRKYGQ
Sbjct: 105 DEFVSSPIGLSLGPIENIT--------NDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQ 164

Query: 157 KVTRDNPSPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYEGEHNHASHFQTELSLRSI 216
           K+TRDNPSPRAYF+CS +P+C VKKKVQRS EDP+ LVATYEG HNH             
Sbjct: 165 KITRDNPSPRAYFRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNHT------------ 224

Query: 217 NGGKGSAVPVLATIKPSCATVTLDLIHEDGLFKSPKDYASSELAVSTEAAVWQEFLVQQM 276
               G    V  T+K       LDL+ + GL           +    E    QE LVQQM
Sbjct: 225 ----GPHASVSRTVK-------LDLV-QGGL---------EPVEEKKERGTIQEVLVQQM 266

Query: 277 ASSLKKDPEFAGIVAGAISDKVL 299
           ASSL KDP+F   +A AIS +++
Sbjct: 285 ASSLTKDPKFTAALATAISGRLI 266

BLAST of Cp4.1LG06g03010 vs. TAIR 10
Match: AT4G01720.1 (WRKY family transcription factor )

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-18
Identity = 73/220 (33.18%), Postives = 102/220 (46.36%), Query Frame = 0

Query: 22  LSLANKAQGSDSS----------RRLEEELDRKMVENGKLSQMLRIMYEKYINLQKQVMC 81
           L L N   G+ S+           RL+ EL+R   EN KL  +L  + E Y +LQ++V+ 
Sbjct: 77  LGLVNSCHGTSSNDGDDKTKTQISRLKLELERLHEENHKLKHLLDEVSESYNDLQRRVLL 136

Query: 82  LVS------HQKQSSEMEALISSRKREEEEEEEEEEEEGICSTR--------DEDFNRWL 141
                    H KQ  ++    SS+  E    ++   E    + +          D +R  
Sbjct: 137 ARQTQVEGLHHKQHEDVPQAGSSQALENRRPKDMNHETPATTLKRRSPDDVDGRDMHRGS 196

Query: 142 -KRPRLNGNPKVS--------------KVFVHKDASDPSLVVKDGYQWRKYGQKVTRDNP 201
            K PR++ N   +              K  V   A   +  V DG QWRKYGQK+ + NP
Sbjct: 197 PKTPRIDQNKSTNHEEQQNPHDQLPYRKARVSVRARSDATTVNDGCQWRKYGQKMAKGNP 256

Query: 202 SPRAYFKCSSAPNCPVKKKVQRSLEDPAILVATYEGEHNH 203
            PRAY++C+ A  CPV+K+VQR  ED  IL  TYEG HNH
Sbjct: 257 CPRAYYRCTMAVGCPVRKQVQRCAEDTTILTTTYEGNHNH 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SAH77.8e-4143.01Probable WRKY transcription factor 40 OS=Arabidopsis thaliana OX=3702 GN=WRKY40 ... [more]
Q9C5T41.9e-3942.96WRKY transcription factor 18 OS=Arabidopsis thaliana OX=3702 GN=WRKY18 PE=1 SV=2[more]
Q6IEK53.4e-3637.41WRKY transcription factor WRKY76 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY7... [more]
Q6IEL04.4e-3638.18WRKY transcription factor WRKY71 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY7... [more]
Q6EPZ25.8e-3637.06WRKY transcription factor WRKY76 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK... [more]
Match NameE-valueIdentityDescription
XP_023535967.11.09e-207100.00probable WRKY transcription factor 40 [Cucurbita pepo subsp. pepo][more]
KAG7024421.12.63e-20298.32putative WRKY transcription factor 40 [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6591536.11.26e-20197.67putative WRKY transcription factor 40, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022975893.19.45e-20196.71probable WRKY transcription factor 40 [Cucurbita maxima][more]
XP_022936945.19.10e-19896.98probable WRKY transcription factor 40 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1IKJ74.58e-20196.71probable WRKY transcription factor 40 OS=Cucurbita maxima OX=3661 GN=LOC11147646... [more]
A0A6J1FF554.41e-19896.98probable WRKY transcription factor 40 OS=Cucurbita moschata OX=3662 GN=LOC111443... [more]
S0F0E61.68e-16784.33WRKY transcription factor OS=Cucumis sativus OX=3659 GN=WRKY32 PE=2 SV=1[more]
A0A0A0L3241.74e-16784.33WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652000 PE=4 S... [more]
A0A5A7VC914.80e-16784.33Putative WRKY transcription factor 40 isoform X1 OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT1G80840.15.5e-4243.01WRKY DNA-binding protein 40 [more]
AT4G31800.11.4e-4042.96WRKY DNA-binding protein 18 [more]
AT4G31800.21.4e-4042.96WRKY DNA-binding protein 18 [more]
AT2G25000.12.5e-3440.68WRKY DNA-binding protein 60 [more]
AT4G01720.11.5e-1833.18WRKY family transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 75..103
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..105
NoneNo IPR availablePANTHERPTHR31429:SF3WRKY TRANSCRIPTION FACTOR 40-RELATEDcoord: 22..299
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 144..204
e-value: 6.9E-35
score: 131.9
IPR003657WRKY domainPFAMPF03106WRKYcoord: 145..202
e-value: 7.4E-24
score: 83.8
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 139..205
score: 25.728949
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 129..204
e-value: 2.5E-28
score: 99.7
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 142..203
IPR044810WRKY transcription factor, plantPANTHERPTHR31429WRKY TRANSCRIPTION FACTOR 36-RELATEDcoord: 22..299

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g03010.1Cp4.1LG06g03010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding