Cp4.1LG06g02960 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g02960
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial
LocationCp4.1LG06: 1603872 .. 1608823 (+)
RNA-Seq ExpressionCp4.1LG06g02960
SyntenyCp4.1LG06g02960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGATGGTCTCAAAATCTCTGAATCAAGAACGAGCGCTTGGCAGCAGCAGGGAGCAAGATTTCTCGAGTCGCTCGAATATTGTATGCGCTTCGGAACTTCGCTTGAAACTTGAAGGTCTGTTCTTAACTTCGAATGACCAGAGACGAGAACAAACCTTGTGGAGTGTAGAAGAATTCACACTGATTTCCAGACAGATAGGGAGAGATGCAGAGATTGAAAGCAGTGGAAGCTGTGAGGAGATCTTCATTTCAGCGTTTTAGCTCTTTTTCTAACCTGAAGAGAGGCTTTTCTTCTCTACCAAACTATGCTCAAACTGATGATGTTTTCGACCAGGTTTGTATTCGGAGTTCTTTTTTAGTTTGTTAACGAGCAATCAAACGCGGAAATGCGCCTCTTTCTATCTGTTTTTTTCTCTCCTAAACTCGATTCATTTTCGGTGGAGGAGCCTACTGTGCAGGTACCGGTTTTTGTTTCTGAAGAGGTGTTTATGTGATCGTTAGATTCTAGTGAAAGGAGGACCGAAGTCTAGAGCAGCTATCCTCAATAGACCATCGGCTCTCAATGCTCACTACTTCCATGGTTCTTTTCCTATTTTTTTATTCTTTGCTTCCTTTCTGTGTTGTGAGATTAGTTTGCTGTTTTGTTTGGTTGTTTGGTTGATAATTGCGTCCTCATTCAAAAATGTTCAACTACGAGTTGTATTTGTCGTAGGTAGCCCGGCTGAACAATCTGTATGAATCGTGGAGGAAAATTCTGATATCGGATTTGTCTTAATGAAGATGTGGACTGATTTATGCGTACATTCACGGCTGTTCAACGTCGAGCACTTTCTCCGATTTAATTGTTATATTTCCAGTGTCAGAAACCTCTTAATTGAAAGTTTAGCTCGTGTTTTTATCATTAGGACTTGTTCTACCGACGTATCTGAGCAGCGTCATTTTATTTTTTGAAGGCTTGTGGCAGGGCGTTTTGCTCCGGTGCAGATGTTGTCGCGCTTAATCATTTCTCAAATGAAGGTGAGAGGTCTTTTGATGTTTCGAATGGAACTTAACAAACTAGTCATTCGCATTGGTTAAGGCGTTCATACGATCATGCAATTTTATACTATTCTGTTTAGCTACGGAGCATTGAGAATCCGCGAAGGCATCAATAGGCTTTGACATTACTTGTTTAATGTTAATTTTGTTCTCGTTATGATTGGTAAATTTTATCAGTTTCTCAGGGAAACTTATATGATTGTCATTTCAGCAAACTTATCAATATGAAACAAATGTATTGAAAAGGATAGAAGATGCAAATGGAGACGAGAGATGACTGGAACTCAAGACCTATAGCATATTAGGGAAATCATGAAAGCATTCTTCAACTGGTCAAAAGTAAGAAAGGCGGCCACTAGCCAAAATGAAGTAAGAAGATTTCACTCATTCCCACACCTTCTCTGCTTTATCCGTTTATCTCAAAATTTGATCTAATTCATATTTCAAGAAATAGAATGAAGAGGAAAACCTCCACATTGTCTATATTGAGACTAGAGAGGAAAATCCTGGAGGGATAGAGGGAAGGAGAAATTGAATTCAATGGATCTCCCTTTTCATCGTAGGAAACCTCACAATAAGTCTTTAATTGTCTCCCTTCACAGATCAACATTACATGTCACACACTTTAGAGTGCTAAGGATTGTTCAAGTAATTTAAAGGAAATATGTGACGACTTTTAATACTTTAGATTCGGAAGCCATTGTCACCACTCTTTATTTAGATAAAGAAGAGAAATCATGGCTCAATGTTCCCGACCGGTTTGGTTGGATGAAATAGAGAAAACTTCAATTGAAAGTTCTTTAAACTTAGTGAATTAGACAGTTCACACTATGGAAATCTTTCCCTCGGTTGGTCCTTCTTTAAGACAATAATCCACGTCCATTGATTTGTATAGTTTTCACACTAATGGCTAACAGGTTTTTTTTATCATCTTACATTCATTATCTCATGCAAATAAGTTAGAGCCTCAACTCACACCCTTTTCCTTTTGGGCACAGGGAAAATCGAAAACTGCAAGAAGTTCTTTGAGACATTATACAGATTTGTGTATCTTGAATGAACTTACTTGAAGCCACATGTAAGTAACAACTCTTCATGTTGTCTGTTGAGATGTTTCGTATAGAGATTGACTACAGGGAGCTCATCAGTGCTTTATATTTCTTCTGCAGGCCTAATGATTCTGTTGAGGTAAAGAGAGTTAGTAAGTGCATTTGCTGATCTTCAGTATCCTGTGTATAAAACTTTGTAGCCTGTAATTATTATTTTTATCAGGTGGCCCTGTTGGACGGTATCATGATGGGAGGTGGGGCTGGGATTACCCTTCCAGGCATGTTCAAAGTGGTGACTGATAGAACTGCAAATACAGGTGCAATTTTTGCAATCACCTTTTTGTGATGTTTTTCTATCCAGATATTTAAATGATTCGTATATGACATTGCTCATTACACACTATCATAACATGATGTTTGAACAAATGAATTTAAATATTTGAAAAGAGAGAAATTTAATAGAACTATATGATTGTCAACAAATGTTTGATAGCTAAACCAGGAATGAACTTTTCATTGATTGATGAAAAGATGCAAAAATTGTCCGAGTAAACAATCCTCTATTTAATACTAAAAGTTACATGTCCTATGTACGACCCGATCGAACACAGTACATTAATAATTGATTTAAGTTGCGGGCGTGGCATCAATTTTGGATTCCATTTTAAGTTGGATGCTTCAAATTAGTTCATTTGGAAGATAAAACTTATAGAGTTAACTTCAAAGTATATTCCTCTGGTGTGCACTATGCGAATTCATTTTTCACGAACTGAATAAAACCTAGAGATAGAAAATCCCATAATTAGTGCACGGATGAAAATTTCTCATGGATAGCTTCCCTTGAAGCATTAAAAAGCCAAAAATTATGAAGGAAATTGAACGTCATCAATGTGGTAAAGCTGTGTGGTTAAGTGGAAGTTCTTATTCTTCTGTTAGACACTATTCGCTCAATGCCGTTAGTTTTCTGTTCCTTCATTCTTTTTTATTTCTCATTCTCATTCATTCATGGCTTGTAATGATCAATCTATGACTTTGGCAGAGACTTGGTGGAATTGAAGAACGCCTTGGTAAAATGATAACCGATGACCCTTCTGTCATAGAATCATCTCTTGCTGCATTCGGTGATCTTGTCGATCCTCCAAAAGTGACCTCGTTTCGCCAGTTCAGGCTCCCGATAACAGGAGCGTTCTTTCCAAGTACTGTTCTTTCTTCTACACCTTCCTCTTTGTTTAGATTAAGTCCTAGCCTCTTCGTATGTTAAACTTAATACAAAAGAAATTGCTTGAAGTTGATTTTACTTTTGAGATTTTGTACTTCCTGGGGCAGTATTAACAACGCTATGCTACGAGAACAGTCGGCTGAGTAAAATAGTGAGCTTCGTCATAAATCATCAACTAGCACACTAGATTTTAACAACCATAGGATAGAATTCATCTCTCACACACGAATGATTGTGGTTTATTACGATTTAAGTGTTCTTTCTTTTCTTCTAGATCTTGATCACTTTCCAAGCCTTTCTTGTTCCACAGGCTCGATGCAACAGATGGGTGTTTTAGCCACGACACTGTTGAGTAAATTTTGGAAGCCTTGGTAAGCATTGTATCATGTTTCTTTCTAAACCTTTATGATGCTGTTGGACACTTCAACTAGTAGAATTCATAGACTAACCAACCAAGTGTATAGCTTGCTGACCTCTACTAAGAATCAAAACTTCACGGAAGTATTTTTTGTTTTAATCCACACGTTTAGGAAAGAGAGGCTGCAGAGTCTTGTGATGAATGGTATTCTTCAACCCTGAAGTTACTTCAGAGAAGCCTCGCCATTGAGCTGAAAGTTACTCTAAATCTGTGAGTTTTCTTGTTTATGTACACGTCTGTTATTCTCTTGGAATACATTCAACTCTCTAATCCTTATTTTATCCCATCGTATTTGTAGATCTGTGAGTTTTCGAAGTCTCAAGGCCAATGTCTTGCTCGTGAATACAGTATATCCCTCATCGGAATTTTGAAGCAGGCATCTCCCGATTTCTATGAGGTTATTAACTTACTAAATTCATTTGGACAGTTCAAATTTGTCATTCTGACGCCTTATTTCTATCATCAATAGGGTGTTCGAGGTCGATAAACCTTTACTTTATTAAATTGTTTGTAATTTCTAAAATTTCAAGTTTATTTCTATCTAACAACTCTTTCAAACTGTCTATTTTTATTTCTTGAGCTCTAAAATTTCAACCTTTACTTTATCCCATCGTATTTTTTTTTTTTTATATCAAATTAGAGGATTTTGAAACTTGATGTATTTAAGAAATGTGTGAGGTAATATAGTTAATATGGTGGACTGCCACTTTTCACGGGCAGGTGAATTACCTGAACTATTGCTGCCCACTGCGTTACGGTATCCAAATTCACGGTAAAGAAGCAAAAATATACCCGACGTCTATTTGGTGGTGCCATAATTATTGTTTCAGACACTCAATTTCTCCGGTTCAGAGGTTGAATTTCATCTTGTCAATGACCATTTTGGTATGACTTTTTTTTTCTCTCTCATATAATATATTATTGTTTTTAATGAGCCCATCGATTTTTCCTAATAGATAGTAGAGGTGTACATTCAACCCGATATACTGAATCAACACAATTTGATGGATTGGATTGGGTTCATTTTTTTGAATCCAAAATGATTCGGTTCGATTTTAAATCCCTTGGATAAAAACTTCGAATTGACCCAAACCAAACCTAAGAAGTTTAGCGTACAAATATTCATGCCCAGGTTTGACTCCAACTAGGAACATTATTTCTTTTTGTTATTTTGTTTAATTTTGTTTATATTATTTTATTATTTTATTATTTTTAGTTTTATAATATT

mRNA sequence

CAGATGGTCTCAAAATCTCTGAATCAAGAACGAGCGCTTGGCAGCAGCAGGGAGCAAGATTTCTCGAGTCGCTCGAATATTGTATGCGCTTCGGAACTTCGCTTGAAACTTGAAGGTCTGTTCTTAACTTCGAATGACCAGAGACGAGAACAAACCTTGTGGAGTGTAGAAGAATTCACACTGATTTCCAGACAGATAGGGAGAGATGCAGAGATTGAAAGCAGTGGAAGCTGTGAGGAGATCTTCATTTCAGCGTTTTAGCTCTTTTTCTAACCTGAAGAGAGGCTTTTCTTCTCTACCAAACTATGCTCAAACTGATGATGTTTTCGACCAGATTCTAGTGAAAGGAGGACCGAAGTCTAGAGCAGCTATCCTCAATAGACCATCGGCTCTCAATGCTCACTACTTCCATGCGTCATTTTATTTTTTGAAGGCTTGTGGCAGGGCGTTTTGCTCCGGTGCAGATGTTGTCGCGCTTAATCATTTCTCAAATGAAGAGATTGACTACAGGGAGCTCATCAGTGCTTTATATTTCTTCTGCAGGCCTAATGATTCTGTTGAGGTGGCCCTGTTGGACGGTATCATGATGGGAGGTGGGGCTGGGATTACCCTTCCAGGCATGTTCAAAGTGGTGACTGATAGAACTGCAAATACAGCTGTGTGGTTAAGTGGAAGTTCTTATTCTTCTGTTAGACACTATTCGCTCAATGCCAGACTTGGTGGAATTGAAGAACGCCTTGGTAAAATGATAACCGATGACCCTTCTGTCATAGAATCATCTCTTGCTGCATTCGGTGATCTTGTCGATCCTCCAAAAGTGACCTCGTTTCGCCAGTTCAGGCTCCCGATAACAGGAGCGTTCTTTCCAAGTACTGTTCTTTCTTCTACACCTTCCTCTTTGTTTAGATTAAGTCCTAGCCTCTTCGAAAGAGAGGCTGCAGAGTCTTGTGATGAATGGTATTCTTCAACCCTGAAGTTACTTCAGAGAAGCCTCGCCATTGAGCTGAAAGTTACTCTAAATCTATCTGTGAGTTTTCGAAGTCTCAAGGCCAATGTCTTGCTCGTGAATACAGTATATCCCTCATCGGAATTTTGAAGCAGGCATCTCCCGATTTCTATGAGGTGAATTACCTGAACTATTGCTGCCCACTGCGTTACGGTATCCAAATTCACGGTAAAGAAGCAAAAATATACCCGACGTCTATTTGGTGGTGCCATAATTATTGTTTCAGACACTCAATTTCTCCGGTTCAGAGGTTGAATTTCATCTTGTCAATGACCATTTTGGTATGACTTTTTTTTTCTCTCTCATATAATATATTATTGTTTTTAATGAGCCCATCGATTTTTCCTAATAGATAGTAGAGGTGTACATTCAACCCGATATACTGAATCAACACAATTTGATGGATTGGATTGGGTTCATTTTTTTGAATCCAAAATGATTCGGTTCGATTTTAAATCCCTTGGATAAAAACTTCGAATTGACCCAAACCAAACCTAAGAAGTTTAGCGTACAAATATTCATGCCCAGGTTTGACTCCAACTAGGAACATTATTTCTTTTTGTTATTTTGTTTAATTTTGTTTATATTATTTTATTATTTTATTATTTTTAGTTTTATAATATT

Coding sequence (CDS)

ATGCAGAGATTGAAAGCAGTGGAAGCTGTGAGGAGATCTTCATTTCAGCGTTTTAGCTCTTTTTCTAACCTGAAGAGAGGCTTTTCTTCTCTACCAAACTATGCTCAAACTGATGATGTTTTCGACCAGATTCTAGTGAAAGGAGGACCGAAGTCTAGAGCAGCTATCCTCAATAGACCATCGGCTCTCAATGCTCACTACTTCCATGCGTCATTTTATTTTTTGAAGGCTTGTGGCAGGGCGTTTTGCTCCGGTGCAGATGTTGTCGCGCTTAATCATTTCTCAAATGAAGAGATTGACTACAGGGAGCTCATCAGTGCTTTATATTTCTTCTGCAGGCCTAATGATTCTGTTGAGGTGGCCCTGTTGGACGGTATCATGATGGGAGGTGGGGCTGGGATTACCCTTCCAGGCATGTTCAAAGTGGTGACTGATAGAACTGCAAATACAGCTGTGTGGTTAAGTGGAAGTTCTTATTCTTCTGTTAGACACTATTCGCTCAATGCCAGACTTGGTGGAATTGAAGAACGCCTTGGTAAAATGATAACCGATGACCCTTCTGTCATAGAATCATCTCTTGCTGCATTCGGTGATCTTGTCGATCCTCCAAAAGTGACCTCGTTTCGCCAGTTCAGGCTCCCGATAACAGGAGCGTTCTTTCCAAGTACTGTTCTTTCTTCTACACCTTCCTCTTTGTTTAGATTAAGTCCTAGCCTCTTCGAAAGAGAGGCTGCAGAGTCTTGTGATGAATGGTATTCTTCAACCCTGAAGTTACTTCAGAGAAGCCTCGCCATTGAGCTGAAAGTTACTCTAAATCTATCTGTGAGTTTTCGAAGTCTCAAGGCCAATGTCTTGCTCGTGAATACAGTATATCCCTCATCGGAATTTTGA

Protein sequence

MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRPSALNAHYFHASFYFLKACGRAFCSGADVVALNHFSNEEIDYRELISALYFFCRPNDSVEVALLDGIMMGGGAGITLPGMFKVVTDRTANTAVWLSGSSYSSVRHYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFEREAAESCDEWYSSTLKLLQRSLAIELKVTLNLSVSFRSLKANVLLVNTVYPSSEF
Homology
BLAST of Cp4.1LG06g02960 vs. ExPASy Swiss-Prot
Match: Q8RXN4 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g31810 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.7e-38
Identity = 125/332 (37.65%), Postives = 173/332 (52.11%), Query Frame = 0

Query: 4   LKAVEAVRRSS--FQRFSSFSNLKRGFSSLPNYAQTD-DVFDQILVKGGPKSRAAILNRP 63
           ++ V+A+RR S   Q   S S  +R FS+LPNY+ +D D  DQ+LV+G  KSRAAILN P
Sbjct: 1   MQTVKALRRVSEPLQWVRSVSYGRR-FSALPNYSASDADFEDQVLVEGKAKSRAAILNNP 60

Query: 64  SALNA----------HYFHA-------SFYFLKACGRAFCSGADVVALNHFSNE------ 123
           S+LNA            + +       SF  +K  G+ FCSGADV++L H  NE      
Sbjct: 61  SSLNALSAPMVGRLKRLYESWEENPAISFVLMKGSGKTFCSGADVLSLYHSINEGNTEES 120

Query: 124 EIDYRELISALYF---FCRPNDSVEVALLDGIMMGGGAGITLPGMFKVVTDRT--ANTAV 183
           ++ +  L   +Y    + +PN    +A++DG+ MG G GI+LPGMF+V TD+T  A+  V
Sbjct: 121 KLFFENLYKFVYLQGTYLKPN----IAIMDGVTMGCGGGISLPGMFRVATDKTVLAHPEV 180

Query: 184 WL-----SGSSYSSVR----------------------------HYSLNARLGGIEERLG 243
            +     +G+SY   R                            HY LNARL  IEER+G
Sbjct: 181 QIGFHPDAGASYYLSRLPGYLGEYLALTGQKLNGVEMIACGLATHYCLNARLPLIEERIG 240

Query: 244 KMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSL 272
           K++TDDP+VIE SLA +GDLV P   +   +  L I   F   TV     +         
Sbjct: 241 KLLTDDPAVIEDSLAQYGDLVYPDSSSVLHKIEL-IDKYFGLDTVEEIIEA--------- 300

BLAST of Cp4.1LG06g02960 vs. ExPASy Swiss-Prot
Match: Q5XF59 (3-hydroxyisobutyryl-CoA hydrolase-like protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g60510 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-24
Identity = 93/292 (31.85%), Postives = 130/292 (44.52%), Query Frame = 0

Query: 37  TDDVFDQILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACG 96
           ++D+  Q+LV+G   SR AILNRP ALNA   H                   F  +K  G
Sbjct: 32  SEDLDYQVLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSG 91

Query: 97  RAFCSGADVVALNHFSNEEID--YRELISALYFFCRPNDSV---EVALLDGIMMGGGAGI 156
           RAFC+G D+V+L H          RE  S+LY F     +     VA+L+G+ MGGG G+
Sbjct: 92  RAFCAGGDIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVTMGGGTGV 151

Query: 157 TLPGMFKVVTDRT--------------------------------ANTAVWLSGSSYSS- 216
           ++PG F+V TDRT                                  T + LSG+   + 
Sbjct: 152 SIPGTFRVATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLSGAEMLAC 211

Query: 217 --VRHYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAF 272
               HY  +  +  +EE+L K++TDDPSV+ES L    ++  P K    R+  L +   F
Sbjct: 212 GLATHYIRSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRIDL-LEKCF 271

BLAST of Cp4.1LG06g02960 vs. ExPASy Swiss-Prot
Match: Q9LKJ1 (3-hydroxyisobutyryl-CoA hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=CHY1 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.6e-14
Identity = 77/294 (26.19%), Postives = 123/294 (41.84%), Query Frame = 0

Query: 43  QILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACGRAFCSG 102
           Q+LV+     R   LNRP  LNA  FH                      LK  GRAFC+G
Sbjct: 10  QVLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAG 69

Query: 103 ADVVAL-------------NHFSNEEIDYRELISALYFFCRPNDSVEVALLDGIMMGGGA 162
            DV A+             N+FS+E +        L +        +V++L+GI+MGGGA
Sbjct: 70  GDVAAVVRDINQGNWRLGANYFSSEYM--------LNYVMATYSKAQVSILNGIVMGGGA 129

Query: 163 GITLPGMFKVVTDRTA----NTAVWL---SGSSYSSVR---------------------- 222
           G+++ G F++ T+ T      TA+ L    G+SY   R                      
Sbjct: 130 GVSVHGRFRIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEML 189

Query: 223 ------HYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITG 272
                 H+  + RL  +E  L ++ ++DP+   + L A+       + +++R  RL +  
Sbjct: 190 ACGLATHFVPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYR--RLDVID 249

BLAST of Cp4.1LG06g02960 vs. ExPASy Swiss-Prot
Match: Q6NMB0 (Probable 3-hydroxyisobutyryl-CoA hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g30660 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.8e-11
Identity = 73/286 (25.52%), Postives = 114/286 (39.86%), Query Frame = 0

Query: 43  QILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACGRAFCSG 102
           Q+LV+     R   LNRP  LNA  F+                      LK  GRAFC+G
Sbjct: 6   QVLVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAG 65

Query: 103 ADV--VALNHFSNE---EIDYRELISALYFFCRPNDSVEVALLDGIMMGGGAGITLPGMF 162
            DV  V  N    +     D+      L +        +V++L+GI+MG GAG+++ G F
Sbjct: 66  GDVPPVVQNMVQGKWRLGADFFRDQYTLNYVMATYSKPQVSILNGIVMGAGAGVSIHGRF 125

Query: 163 KVVTDRTA----NTAVWL---SGSSYSSVR----------------------------HY 222
           ++ T+ T      T++ L    G+SY   R                            H+
Sbjct: 126 RIATENTVFAMPETSLGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAELLACGLATHF 185

Query: 223 SLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVL 272
             + RL  +E  L K+ + DPS + + L A+     P         RL +    F    +
Sbjct: 186 VPSTRLTALETDLCKVGSSDPSFVSTILDAYTQ--HPHLKQKSAYHRLDVIDRCFSKRTM 245

BLAST of Cp4.1LG06g02960 vs. ExPASy Swiss-Prot
Match: Q9SHJ8 (3-hydroxyisobutyryl-CoA hydrolase-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=At1g06550 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 4.8e-11
Identity = 73/294 (24.83%), Postives = 116/294 (39.46%), Query Frame = 0

Query: 35  AQTDDVFDQILVKGGPKS--RAAILNRPSALN----------AHYF-------HASFYFL 94
           AQ     D+ +V G  K   R   LNRP  LN          A Y              +
Sbjct: 2   AQEGQNIDEPVVIGEEKGSVRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLILI 61

Query: 95  KACGRAFCSGADVVALNHFSNEEIDYRELISALYFFC---RPNDSVEVALLDGIMMGGGA 154
           K  GRAF +G D+    H    +    E++  +Y+ C         +V+L++GI MGGGA
Sbjct: 62  KGTGRAFSAGGDLKVFYHGQESKDSCLEVVYRMYWLCYHIHTYKKTQVSLVNGISMGGGA 121

Query: 155 GITLPGMFKVVTDRT--------------------------------ANTAVWLSGSSYS 214
            + +P  F VVT++T                                A T   L+G    
Sbjct: 122 ALMVPMKFSVVTEKTVFATPEASFGFHTDCGFSYIHSRLPGHLGEFLALTGARLNGKELV 181

Query: 215 SV---RHYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITG 272
           ++    H+  + +L  +E RL  + + D  V++S++  F + V+  K  S    +  I  
Sbjct: 182 AIGMATHFVPSGKLMDLEARLVSLDSGDADVVQSTIEEFSEKVNLDK-DSILNKQSVINE 241

BLAST of Cp4.1LG06g02960 vs. NCBI nr
Match: KAG7024416.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 329 bits (844), Expect = 3.74e-110
Identity = 196/290 (67.59%), Postives = 200/290 (68.97%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQ                 
Sbjct: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQ----------------- 60

Query: 61  SALNAHYFHASFYFLKACGRAFCSGADVVALNHFSNEEIDYRELISALYFFC-------- 120
                           A GRAFCSGADV+A  HFSNE  D   +++ LY           
Sbjct: 61  ----------------ASGRAFCSGADVIAPYHFSNE--DPEAIVTTLYLDKEEKSWLNV 120

Query: 121 --RPNDSVEVALLDGIMMGGGAGITLPGMFKVVTDRTANTAVWLSGSSYSSVRHYSLNAR 180
             RPNDSVEVALLDGIMM GGAGITLPGMFKVVTD TA   VWLSGSS SSVRHYSLNAR
Sbjct: 121 PDRPNDSVEVALLDGIMMRGGAGITLPGMFKVVTDGTAT--VWLSGSSCSSVRHYSLNAR 180

Query: 181 LGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPS 240
           LGGIEERLGKMITD PSVIESSLAAFGDLV PPKVTSFRQFRL ITGAFFPSTVLSS PS
Sbjct: 181 LGGIEERLGKMITDGPSVIESSLAAFGDLVYPPKVTSFRQFRLLITGAFFPSTVLSSAPS 240

Query: 241 SLFRLSP---------------------SLFEREAAESCDEWYSSTLKLL 259
           SLFRLSP                        EREAAES DEWYSSTLKLL
Sbjct: 241 SLFRLSPIASTLDATARCFSHDTVEEILEALEREAAESYDEWYSSTLKLL 253

BLAST of Cp4.1LG06g02960 vs. NCBI nr
Match: XP_038896618.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 269 bits (688), Expect = 2.20e-84
Identity = 175/328 (53.35%), Postives = 195/328 (59.45%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQR KA+EAVRRSSFQRFSSFSNLKRGFS+LPNYAQTDD+ DQ+LV+G PKSRAAILNRP
Sbjct: 1   MQRWKALEAVRRSSFQRFSSFSNLKRGFSALPNYAQTDDLSDQVLVEGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                       F  +K  GRAFCSGADVVAL H SNE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSDIGFVLMKGSGRAFCSGADVVALYHLSNEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           ++    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTDRT           
Sbjct: 121 KKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARLGGIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMIVCRLATHYSLNARLGGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P    S       I   F   TV     +          E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDSRRSVLSKLDAIDKCFSHDTVEEILEA---------LEKE 300

BLAST of Cp4.1LG06g02960 vs. NCBI nr
Match: XP_022141033.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Momordica charantia])

HSP 1 Score: 253 bits (645), Expect = 6.43e-78
Identity = 165/328 (50.30%), Postives = 192/328 (58.54%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQR KA+EAVRRSSF R   FSNL+RGFS+LP+Y QTDD+ DQ+LV+G PKSRAAILNRP
Sbjct: 1   MQRFKALEAVRRSSFHRLCYFSNLRRGFSALPSYVQTDDLSDQVLVEGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                    +  F  +K  GRAFCSGADVV L H SNE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSNIGFVLMKGSGRAFCSGADVVNLYHLSNEGNVEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           ++    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTD+T           
Sbjct: 121 KKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDKTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARLGGIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMLVCGLATHYSLNARLGGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P + +   +    I   F   TV     +          E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDRRSVLSKLDA-IDRCFCHDTVEEIMEA---------LEKE 300

BLAST of Cp4.1LG06g02960 vs. NCBI nr
Match: XP_022981840.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 250 bits (639), Expect = 4.84e-77
Identity = 166/328 (50.61%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKA+EAVR SSF+R  SFSNL+RGFS+LPNYAQT+D+ DQ++VKG PKSRAAILNRP
Sbjct: 1   MQRLKALEAVR-SSFRRSCSFSNLRRGFSALPNYAQTEDLSDQVIVKGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           S LNA                   F   F  +K  GRAFCSGADVVAL + SNE    D 
Sbjct: 61  SVLNALTTSMVARLSRLYESWEENFDIGFVLMKGSGRAFCSGADVVALYNLSNEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           R+    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTDRT           
Sbjct: 121 RKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARL GIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMIVCGLATHYSLNARLSGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P + +     +L      F    +     +L        E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDRRSVLS--KLDAIDRCFGLDTVEEILEAL--------EKE 300

BLAST of Cp4.1LG06g02960 vs. NCBI nr
Match: KAG7037652.1 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 249 bits (635), Expect = 1.93e-76
Identity = 166/328 (50.61%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKA+EAVR SSF+R  SFSNL+RGFS+LPNYAQT+D+ DQ++VKG PKSRAAILNRP
Sbjct: 1   MQRLKALEAVR-SSFRRSCSFSNLRRGFSALPNYAQTEDLSDQVIVKGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                       F  +K  GRAFCSGADVVAL + SNE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSDIGFVLMKGSGRAFCSGADVVALYNLSNEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           R+    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTDRT           
Sbjct: 121 RKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARL GIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMIVCGLATHYSLNARLSGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P + +     +L      F    +     +L        E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDRRSVLS--KLDAIDRCFGLDTVEEILEAL--------EKE 300

BLAST of Cp4.1LG06g02960 vs. ExPASy TrEMBL
Match: A0A6J1CJD2 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011534 PE=3 SV=1)

HSP 1 Score: 253 bits (645), Expect = 3.11e-78
Identity = 165/328 (50.30%), Postives = 192/328 (58.54%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQR KA+EAVRRSSF R   FSNL+RGFS+LP+Y QTDD+ DQ+LV+G PKSRAAILNRP
Sbjct: 1   MQRFKALEAVRRSSFHRLCYFSNLRRGFSALPSYVQTDDLSDQVLVEGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                    +  F  +K  GRAFCSGADVV L H SNE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSNIGFVLMKGSGRAFCSGADVVNLYHLSNEGNVEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           ++    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTD+T           
Sbjct: 121 KKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDKTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARLGGIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMLVCGLATHYSLNARLGGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P + +   +    I   F   TV     +          E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDRRSVLSKLDA-IDRCFCHDTVEEIMEA---------LEKE 300

BLAST of Cp4.1LG06g02960 vs. ExPASy TrEMBL
Match: A0A6J1J0Q8 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111480866 PE=3 SV=1)

HSP 1 Score: 250 bits (639), Expect = 2.34e-77
Identity = 166/328 (50.61%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKA+EAVR SSF+R  SFSNL+RGFS+LPNYAQT+D+ DQ++VKG PKSRAAILNRP
Sbjct: 1   MQRLKALEAVR-SSFRRSCSFSNLRRGFSALPNYAQTEDLSDQVIVKGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           S LNA                   F   F  +K  GRAFCSGADVVAL + SNE    D 
Sbjct: 61  SVLNALTTSMVARLSRLYESWEENFDIGFVLMKGSGRAFCSGADVVALYNLSNEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           R+    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTDRT           
Sbjct: 121 RKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARL GIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMIVCGLATHYSLNARLSGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P + +     +L      F    +     +L        E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDRRSVLS--KLDAIDRCFGLDTVEEILEAL--------EKE 300

BLAST of Cp4.1LG06g02960 vs. ExPASy TrEMBL
Match: A0A5A7VFV8 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003030 PE=3 SV=1)

HSP 1 Score: 247 bits (630), Expect = 5.56e-76
Identity = 160/329 (48.63%), Postives = 192/329 (58.36%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKA+E VRRSSFQRFSSFSNLKR FS+LPNYAQTDD+ DQ+LV+G P SRAAILNRP
Sbjct: 1   MQRLKALEVVRRSSFQRFSSFSNLKRSFSALPNYAQTDDLSDQVLVEGRPNSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                       F  +K  GRAFCSG DVVAL + SNE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSDIGFVLMKGSGRAFCSGPDVVALYNLSNEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           ++    LY F     +     VALLDGIMMG GAGI LPG+F++VTDRT           
Sbjct: 121 KKFFETLYKFVYLQGTFLKPHVALLDGIMMGAGAGIVLPGLFRLVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS------GSSYSSVR----------------HYSLNARLGGIEERLGKMI 240
              A  + +LS      GS Y ++                 HYSLNAR  GI+ERLGK+I
Sbjct: 181 HPDAGASFYLSRLPGYLGSEYLALTGDKLNGVEMIACGLATHYSLNARFAGIDERLGKLI 240

Query: 241 TDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFER 271
           TDDPSVIESSLAA+GD+V P + +    ++L      F    L     +L        ++
Sbjct: 241 TDDPSVIESSLAAYGDIVYPDRRSVL--YKLDAIDKCFSHDTLEEILEAL--------KK 300

BLAST of Cp4.1LG06g02960 vs. ExPASy TrEMBL
Match: A0A6J1FQ27 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111445940 PE=3 SV=1)

HSP 1 Score: 246 bits (628), Expect = 1.05e-75
Identity = 164/328 (50.00%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKA+EAVR SSF+R  SFSNL+RGFS+LPNYA+T+D+ DQ++VKG PKSRAAILNRP
Sbjct: 1   MQRLKALEAVR-SSFRRSCSFSNLRRGFSALPNYAKTEDLSDQVIVKGRPKSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                       F  +K  GRAFCSGADVVAL + +NE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSDIGFVLMKGSGRAFCSGADVVALYNLANEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           R+    LY F     +     VALLDGIMMGGGAGI+LPGMFKVVTDRT           
Sbjct: 121 RKFFETLYKFVYLQGTYLKPHVALLDGIMMGGGAGISLPGMFKVVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNARL GIEERLGKMIT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMIVCGLATHYSLNARLSGIEERLGKMIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GDLV P + +     +L      F    +     +L        E+E
Sbjct: 241 DDPSVIESSLAAYGDLVYPDRRSVLS--KLDAIDRCFGLDTVEEILEAL--------EKE 300

BLAST of Cp4.1LG06g02960 vs. ExPASy TrEMBL
Match: A0A1S3BVM5 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493749 PE=3 SV=1)

HSP 1 Score: 245 bits (625), Expect = 3.04e-75
Identity = 159/328 (48.48%), Postives = 190/328 (57.93%), Query Frame = 0

Query: 1   MQRLKAVEAVRRSSFQRFSSFSNLKRGFSSLPNYAQTDDVFDQILVKGGPKSRAAILNRP 60
           MQRLKA+E VRRSSFQRFSSFSNLKR FS+LPNYAQTDD+ DQ+LV+G P SRAAILNRP
Sbjct: 1   MQRLKALEVVRRSSFQRFSSFSNLKRSFSALPNYAQTDDLSDQVLVEGRPNSRAAILNRP 60

Query: 61  SALNA-----------------HYFHASFYFLKACGRAFCSGADVVALNHFSNEEI--DY 120
           SALNA                       F  +K  GRAFCSG DVVAL + SNE    D 
Sbjct: 61  SALNALTTSMVARLNRLYESWEENSDIGFVLMKGSGRAFCSGPDVVALYNLSNEGNIEDC 120

Query: 121 RELISALYFFCRPNDSV---EVALLDGIMMGGGAGITLPGMFKVVTDRT----------- 180
           ++    LY F     +     VALLDGIMMG GAGI LPG+F++VTDRT           
Sbjct: 121 KKFFETLYKFVYLQGTFLKPHVALLDGIMMGAGAGIVLPGLFRLVTDRTVFSHPEVRMGF 180

Query: 181 ---ANTAVWLS-------------GSSYSSVR--------HYSLNARLGGIEERLGKMIT 240
              A  + +LS             G   + V         HYSLNAR  GI+ERLGK+IT
Sbjct: 181 HPDAGASFYLSRLPGYLGEYLALTGDKLNGVEMIACGLATHYSLNARFAGIDERLGKLIT 240

Query: 241 DDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSLFERE 271
           DDPSVIESSLAA+GD+V P + +    ++L      F    L     +L        ++E
Sbjct: 241 DDPSVIESSLAAYGDIVYPDRRSVL--YKLDAIDKCFSHDTLEEILEAL--------KKE 300

BLAST of Cp4.1LG06g02960 vs. TAIR 10
Match: AT4G31810.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.9e-39
Identity = 125/332 (37.65%), Postives = 173/332 (52.11%), Query Frame = 0

Query: 4   LKAVEAVRRSS--FQRFSSFSNLKRGFSSLPNYAQTD-DVFDQILVKGGPKSRAAILNRP 63
           ++ V+A+RR S   Q   S S  +R FS+LPNY+ +D D  DQ+LV+G  KSRAAILN P
Sbjct: 1   MQTVKALRRVSEPLQWVRSVSYGRR-FSALPNYSASDADFEDQVLVEGKAKSRAAILNNP 60

Query: 64  SALNA----------HYFHA-------SFYFLKACGRAFCSGADVVALNHFSNE------ 123
           S+LNA            + +       SF  +K  G+ FCSGADV++L H  NE      
Sbjct: 61  SSLNALSAPMVGRLKRLYESWEENPAISFVLMKGSGKTFCSGADVLSLYHSINEGNTEES 120

Query: 124 EIDYRELISALYF---FCRPNDSVEVALLDGIMMGGGAGITLPGMFKVVTDRT--ANTAV 183
           ++ +  L   +Y    + +PN    +A++DG+ MG G GI+LPGMF+V TD+T  A+  V
Sbjct: 121 KLFFENLYKFVYLQGTYLKPN----IAIMDGVTMGCGGGISLPGMFRVATDKTVLAHPEV 180

Query: 184 WL-----SGSSYSSVR----------------------------HYSLNARLGGIEERLG 243
            +     +G+SY   R                            HY LNARL  IEER+G
Sbjct: 181 QIGFHPDAGASYYLSRLPGYLGEYLALTGQKLNGVEMIACGLATHYCLNARLPLIEERIG 240

Query: 244 KMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAFFPSTVLSSTPSSLFRLSPSL 272
           K++TDDP+VIE SLA +GDLV P   +   +  L I   F   TV     +         
Sbjct: 241 KLLTDDPAVIEDSLAQYGDLVYPDSSSVLHKIEL-IDKYFGLDTVEEIIEA--------- 300

BLAST of Cp4.1LG06g02960 vs. TAIR 10
Match: AT3G60510.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.6e-25
Identity = 93/292 (31.85%), Postives = 130/292 (44.52%), Query Frame = 0

Query: 37  TDDVFDQILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACG 96
           ++D+  Q+LV+G   SR AILNRP ALNA   H                   F  +K  G
Sbjct: 32  SEDLDYQVLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSG 91

Query: 97  RAFCSGADVVALNHFSNEEID--YRELISALYFFCRPNDSV---EVALLDGIMMGGGAGI 156
           RAFC+G D+V+L H          RE  S+LY F     +     VA+L+G+ MGGG G+
Sbjct: 92  RAFCAGGDIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVTMGGGTGV 151

Query: 157 TLPGMFKVVTDRT--------------------------------ANTAVWLSGSSYSS- 216
           ++PG F+V TDRT                                  T + LSG+   + 
Sbjct: 152 SIPGTFRVATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLSGAEMLAC 211

Query: 217 --VRHYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAF 272
               HY  +  +  +EE+L K++TDDPSV+ES L    ++  P K    R+  L +   F
Sbjct: 212 GLATHYIRSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRIDL-LEKCF 271

BLAST of Cp4.1LG06g02960 vs. TAIR 10
Match: AT3G60510.2 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.6e-25
Identity = 93/292 (31.85%), Postives = 130/292 (44.52%), Query Frame = 0

Query: 37  TDDVFDQILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACG 96
           ++D+  Q+LV+G   SR AILNRP ALNA   H                   F  +K  G
Sbjct: 32  SEDLDYQVLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSG 91

Query: 97  RAFCSGADVVALNHFSNEEID--YRELISALYFFCRPNDSV---EVALLDGIMMGGGAGI 156
           RAFC+G D+V+L H          RE  S+LY F     +     VA+L+G+ MGGG G+
Sbjct: 92  RAFCAGGDIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVTMGGGTGV 151

Query: 157 TLPGMFKVVTDRT--------------------------------ANTAVWLSGSSYSS- 216
           ++PG F+V TDRT                                  T + LSG+   + 
Sbjct: 152 SIPGTFRVATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLSGAEMLAC 211

Query: 217 --VRHYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAF 272
               HY  +  +  +EE+L K++TDDPSV+ES L    ++  P K    R+  L +   F
Sbjct: 212 GLATHYIRSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRIDL-LEKCF 271

BLAST of Cp4.1LG06g02960 vs. TAIR 10
Match: AT3G60510.3 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 110.5 bits (275), Expect = 2.3e-24
Identity = 96/308 (31.17%), Postives = 135/308 (43.83%), Query Frame = 0

Query: 37  TDDVFDQILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACG 96
           ++D+  Q+LV+G   SR AILNRP ALNA   H                   F  +K  G
Sbjct: 32  SEDLDYQVLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSG 91

Query: 97  RAFCSGADVVALNHFSNEEID--YRELISALYFFCRPNDSV---EVALLDGIMMGGGAGI 156
           RAFC+G D+V+L H          RE  S+LY F     +     VA+L+G+ MGGG G+
Sbjct: 92  RAFCAGGDIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVTMGGGTGV 151

Query: 157 TLPGMFKVVTDRT--------------------------------ANTAVWLSGSSYSS- 216
           ++PG F+V TDRT                                  T + LSG+   + 
Sbjct: 152 SIPGTFRVATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLSGAEMLAC 211

Query: 217 --VRHYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITGAF 272
               HY  +  +  +EE+L K++TDDPSV+ES L    ++  P K    R+  L +   F
Sbjct: 212 GLATHYIRSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRIDL-LEKCF 271

BLAST of Cp4.1LG06g02960 vs. TAIR 10
Match: AT5G65940.1 (beta-hydroxyisobutyryl-CoA hydrolase 1 )

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-15
Identity = 77/294 (26.19%), Postives = 123/294 (41.84%), Query Frame = 0

Query: 43  QILVKGGPKSRAAILNRPSALNAHYFH-----------------ASFYFLKACGRAFCSG 102
           Q+LV+     R   LNRP  LNA  FH                      LK  GRAFC+G
Sbjct: 10  QVLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAG 69

Query: 103 ADVVAL-------------NHFSNEEIDYRELISALYFFCRPNDSVEVALLDGIMMGGGA 162
            DV A+             N+FS+E +        L +        +V++L+GI+MGGGA
Sbjct: 70  GDVAAVVRDINQGNWRLGANYFSSEYM--------LNYVMATYSKAQVSILNGIVMGGGA 129

Query: 163 GITLPGMFKVVTDRTA----NTAVWL---SGSSYSSVR---------------------- 222
           G+++ G F++ T+ T      TA+ L    G+SY   R                      
Sbjct: 130 GVSVHGRFRIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEML 189

Query: 223 ------HYSLNARLGGIEERLGKMITDDPSVIESSLAAFGDLVDPPKVTSFRQFRLPITG 272
                 H+  + RL  +E  L ++ ++DP+   + L A+       + +++R  RL +  
Sbjct: 190 ACGLATHFVPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYR--RLDVID 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RXN42.7e-3837.653-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Arabidopsis t... [more]
Q5XF592.2e-2431.853-hydroxyisobutyryl-CoA hydrolase-like protein 1, mitochondrial OS=Arabidopsis t... [more]
Q9LKJ14.6e-1426.193-hydroxyisobutyryl-CoA hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=CHY1 PE=1... [more]
Q6NMB04.8e-1125.52Probable 3-hydroxyisobutyryl-CoA hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SHJ84.8e-1124.833-hydroxyisobutyryl-CoA hydrolase-like protein 5 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
KAG7024416.13.74e-11067.593-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Cucurbita argyr... [more]
XP_038896618.12.20e-8453.353-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Benincasa hispi... [more]
XP_022141033.16.43e-7850.303-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Momordica chara... [more]
XP_022981840.14.84e-7750.613-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Cucurbita maxim... [more]
KAG7037652.11.93e-7650.613-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
A0A6J1CJD23.11e-7850.303-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Momordica cha... [more]
A0A6J1J0Q82.34e-7750.613-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Cucurbita max... [more]
A0A5A7VFV85.56e-7648.633-hydroxyisobutyryl-CoA hydrolase-like protein 2 OS=Cucumis melo var. makuwa OX=... [more]
A0A6J1FQ271.05e-7550.003-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Cucurbita mos... [more]
A0A1S3BVM53.04e-7548.483-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Cucumis melo ... [more]
Match NameE-valueIdentityDescription
AT4G31810.11.9e-3937.65ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT3G60510.11.6e-2531.85ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT3G60510.21.6e-2531.85ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT3G60510.32.3e-2431.17ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT5G65940.13.3e-1526.19beta-hydroxyisobutyryl-CoA hydrolase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR045004Enoyl-CoA hydratase/isomerase domainPFAMPF16113ECH_2coord: 72..148
e-value: 1.6E-11
score: 44.3
NoneNo IPR availableGENE3D3.90.226.10coord: 39..151
e-value: 2.3E-9
score: 38.7
NoneNo IPR availablePANTHERPTHR43176:SF143-HYDROXYISOBUTYRYL-COA HYDROLASE-LIKE PROTEIN 2, MITOCHONDRIALcoord: 1..148
NoneNo IPR availablePANTHERPTHR43176:SF143-HYDROXYISOBUTYRYL-COA HYDROLASE-LIKE PROTEIN 2, MITOCHONDRIALcoord: 163..272
IPR032259Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H typePANTHERPTHR431763-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATEDcoord: 1..148
coord: 163..272
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 54..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g02960.1Cp4.1LG06g02960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003860 3-hydroxyisobutyryl-CoA hydrolase activity