Cp4.1LG05g14930 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g14930
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Regulator of Vps4 activity in the MVB pathway protein
Location	Cp4.1LG05: 10277201 .. 10279618 (+)
RNA-Seq Expression	Cp4.1LG05g14930
Synteny	Cp4.1LG05g14930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGGCATCTTCTTCGGATGGCGAAAAGCTTCCAAATGGTAATGAAAATGTTGCAGACTTTCAAGCTTGTTCTTGTTACATGGGTTTGTCGTTTTGATTTGCCTGTCTTGGCTGCAGTAAGAAGCTGATCAAACAAGTTCAATGCCGCCTCAAGCTGCTGAAGAACAAAAAGGGTATAATCACGAAGCAAATGAAGGAAGATGTCGTGCAACTCATTAGAAATGGGTATGATCAAACCGCCTTCAACAGGGTACGTCAAAAAAACAACCAAATTGTTTCAAAAGCTTCCAACTTTGATCAAATTGTTTGATTCCAAAGAACACCCATGAATTAAAATCCTCTGTTTCAGGTGGAGCAGATAGTAAAAGATGAAAACAGAGTGGCGGCGTATGAAATTTTGGACAATTTCTGTGAATTCATACTCCTAAATCTATCTTATATTCGCAAACACAAGTACAGTTTTTACAGTTTGATTCTCGAATGAATTCATTAGCTTTTTGAACTGTTTCTTATTACCAAGATTTGGTGTCGTGTTTAGGGACTGTCCCAATGATGTTAATGAAGCAGTTTCAAGCCTCATGTTTGCCGCGGCGAGATGTGGGGATCTTCCTGAGCTGCAGCATATAAGGAAACTGTTTGGAGAGCGTTATGGGCGTAGGTTTGAAAGTGCTGCCGTTCAGCTTGGTCCAGGAAACCTTGTAAATTGCCAGGTGCAGTAAAGTTCATATTATGCTCTGTTTTGGTCCCAGATTAGTTTGAGGATTAGCTAATTAGCCAACTAATTTTGTTTACAGATAAAAGAGAAGCTCTTAATAAATTCTGTGTCGGATGATGATAAGCAGAGAATGATGAATGAAATTGCTAGAGATTGCCTCCAACCTCAGCTTTTAGCTCTACAATACCGTTCAGATTGGCACCAAAATCAGGTCGGTTGAACCCGTTTCACCGTAGTTTTCATATTTCTTAAGAAAACATTTGAAATTCTTGGTCAAGACAATAAGAAAACGTAGCCATACAAGTTCACAGCTAATAAATATTGTTCTGATATCATGTCAAGAATGATACTTTGATACCATGTAAAGAATAATGAAACATAAGTCGGATCGAAAGTCCAAGAAATGGTATCGATCCCACCTCTATTTTTGGGACTTTCGATCCCACTTATGTTTTATTATTCTTAACAGTTACGTATCGTCGTCAGCCTCACGGTTTTAAAATATGTCTTGTTAGTAGCGATATATAACGAGTCAAAACGGACAATATTTGCTAGCGGTGGACTTAAACGGTTACAAAATGGTATCAGAGCCCGACATCTAGCGGTGTGCCAACGAGAACGTTAAGCTCCCAAAAAGAGTGGATTGTGAGATCCCACGTCGGCTGAAGAGGGGAACGAAGCATTCCTGATAAGGGGTGTGGAAACCTCTTCTTAGCTTCATTCCTAATAAGAATGTGGAAACATCTCCCCCAGTAGACACGTTTTAAAATTTTGAGGGGAAGCTCGAAAAGGAAATTTCTAAGAAGACAATATGGGCTAGTGGTGAACTAGACCGTTACAGAAAACATTTCAAATTTTAAAACATTTTCTTGTTTATCGGTCATTGTGGAACAACTCAGGTAGTGGCAAGGATTGCAAAAGCTGATGAAAAACCAGACCAACACATAAACCAAGCTAATTCAGGAACAAGCAGTTCTTGTGACAGTTTGCCTCAGTTCCCAGAGGAAAGGATTGTTTATCTTGACGATGTGGTTGAGCTATGTTCTTCAACCAACATAGAGGGAGATCAGAGACTCTTCAAATTCAAGACAGCCGCCACTCTCCCAAAGACAGAGATTACCCAACATAGTTCCAACCAAACTGACACTGATCCAACTGAATCTCGTACTGACATAGAGAATTCCAGCTCAAAAATCTCTGTCAAAGGATCAATGGGGACTGAGGGAAAGCACCAAATCAAAGGCAAAAACTTTACAACCGTTCAAAATGGATCAGAAGGAGCAACAGATAAGGAAATGGAATGGGCAAGTTTCTACAAGAAACCAGGAAGGAGGAGGACAAAGAGAAGTGAGCTGCCATCAAGCTCTGATCTGAGTTCTACGACATATGATGTCTTCACTTATAATGGCAACAAGAAAGTTGCAGCAAATGATACAAAATCAAACGCCATGGAGAATTGTGAAGAGATGAAGCTTTCAAGTTCATTGAGGAACAAAAGGGAGGCTCTTTATTTAAGGGCAGAGACAATGCCAACAGAACGACCAAAAGAGAGCCAGAAAGTGAATTTTTGTCGTACAAATTCATGCCCAGATAAGAAACCTACCCATGTTCATCCAAAGTTGCCAGATTATGATGATATTGCAGCAAAGTTCATGGCTTTGAGAAGACAACACCTTCAAAATAATACCCTCAAATCTTAG

mRNA sequence

ATGTTTGGCATCTTCTTCGGATGGCGAAAAGCTTCCAAATGTAAGAAGCTGATCAAACAAGTTCAATGCCGCCTCAAGCTGCTGAAGAACAAAAAGGGTATAATCACGAAGCAAATGAAGGAAGATGTCGTGCAACTCATTAGAAATGGGTATGATCAAACCGCCTTCAACAGGGTGGAGCAGATAGTAAAAGATGAAAACAGAGTGGCGGCGTATGAAATTTTGGACAATTTCTGTGAATTCATACTCCTAAATCTATCTTATATTCGCAAACACAAGGACTGTCCCAATGATGTTAATGAAGCAGTTTCAAGCCTCATGTTTGCCGCGGCGAGATGTGGGGATCTTCCTGAGCTGCAGCATATAAGGAAACTGTTTGGAGAGCGTTATGGGCGTAGGTTTGAAAGTGCTGCCGTTCAGCTTGGTCCAGGAAACCTTGTAAATTGCCAGGTAGTGGCAAGGATTGCAAAAGCTGATGAAAAACCAGACCAACACATAAACCAAGCTAATTCAGGAACAAGCAGTTCTTGTGACAGTTTGCCTCAGTTCCCAGAGGAAAGGATTGTTTATCTTGACGATGTGGTTGAGCTATGTTCTTCAACCAACATAGAGGGAGATCAGAGACTCTTCAAATTCAAGACAGCCGCCACTCTCCCAAAGACAGAGATTACCCAACATAGTTCCAACCAAACTGACACTGATCCAACTGAATCTCGTACTGACATAGAGAATTCCAGCTCAAAAATCTCTGTCAAAGGATCAATGGGGACTGAGGGAAAGCACCAAATCAAAGGCAAAAACTTTACAACCGTTCAAAATGGATCAGAAGGAGCAACAGATAAGGAAATGGAATGGGCAAGTTTCTACAAGAAACCAGGAAGGAGGAGGACAAAGAGAAGTGAGCTGCCATCAAGCTCTGATCTGAGTTCTACGACATATGATGTCTTCACTTATAATGGCAACAAGAAAGTTGCAGCAAATGATACAAAATCAAACGCCATGGAGAATTGTGAAGAGATGAAGCTTTCAAGTTCATTGAGGAACAAAAGGGAGGCTCTTTATTTAAGGGCAGAGACAATGCCAACAGAACGACCAAAAGAGAGCCAGAAAGTGAATTTTTGTCGTACAAATTCATGCCCAGATAAGAAACCTACCCATGTTCATCCAAAGTTGCCAGATTATGATGATATTGCAGCAAAGTTCATGGCTTTGAGAAGACAACACCTTCAAAATAATACCCTCAAATCTTAG

Coding sequence (CDS)

ATGTTTGGCATCTTCTTCGGATGGCGAAAAGCTTCCAAATGTAAGAAGCTGATCAAACAAGTTCAATGCCGCCTCAAGCTGCTGAAGAACAAAAAGGGTATAATCACGAAGCAAATGAAGGAAGATGTCGTGCAACTCATTAGAAATGGGTATGATCAAACCGCCTTCAACAGGGTGGAGCAGATAGTAAAAGATGAAAACAGAGTGGCGGCGTATGAAATTTTGGACAATTTCTGTGAATTCATACTCCTAAATCTATCTTATATTCGCAAACACAAGGACTGTCCCAATGATGTTAATGAAGCAGTTTCAAGCCTCATGTTTGCCGCGGCGAGATGTGGGGATCTTCCTGAGCTGCAGCATATAAGGAAACTGTTTGGAGAGCGTTATGGGCGTAGGTTTGAAAGTGCTGCCGTTCAGCTTGGTCCAGGAAACCTTGTAAATTGCCAGGTAGTGGCAAGGATTGCAAAAGCTGATGAAAAACCAGACCAACACATAAACCAAGCTAATTCAGGAACAAGCAGTTCTTGTGACAGTTTGCCTCAGTTCCCAGAGGAAAGGATTGTTTATCTTGACGATGTGGTTGAGCTATGTTCTTCAACCAACATAGAGGGAGATCAGAGACTCTTCAAATTCAAGACAGCCGCCACTCTCCCAAAGACAGAGATTACCCAACATAGTTCCAACCAAACTGACACTGATCCAACTGAATCTCGTACTGACATAGAGAATTCCAGCTCAAAAATCTCTGTCAAAGGATCAATGGGGACTGAGGGAAAGCACCAAATCAAAGGCAAAAACTTTACAACCGTTCAAAATGGATCAGAAGGAGCAACAGATAAGGAAATGGAATGGGCAAGTTTCTACAAGAAACCAGGAAGGAGGAGGACAAAGAGAAGTGAGCTGCCATCAAGCTCTGATCTGAGTTCTACGACATATGATGTCTTCACTTATAATGGCAACAAGAAAGTTGCAGCAAATGATACAAAATCAAACGCCATGGAGAATTGTGAAGAGATGAAGCTTTCAAGTTCATTGAGGAACAAAAGGGAGGCTCTTTATTTAAGGGCAGAGACAATGCCAACAGAACGACCAAAAGAGAGCCAGAAAGTGAATTTTTGTCGTACAAATTCATGCCCAGATAAGAAACCTACCCATGTTCATCCAAAGTTGCCAGATTATGATGATATTGCAGCAAAGTTCATGGCTTTGAGAAGACAACACCTTCAAAATAATACCCTCAAATCTTAG

Protein sequence

MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIRKLFGERYGRRFESAAVQLGPGNLVNCQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMGTEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDDIAAKFMALRRQHLQNNTLKS

Homology

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.1e-12
Identity = 42/150 (28.00%), Postives = 84/150 (56.00%), Query Frame = 0


Query: 13  KCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRVAAY 72
           K K  +K    R+++LKNKK  I +  K +V +L+R   +++A  RVE I++DE  +  +
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  EILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIRKLFGERYGR 132
           +I++  CE +   ++ I    + P ++ E++ +L++++ R   +PEL+ I+     +YG+
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 RFESAAVQLGPGNLVNCQVVARIAKADEKP 163
             E+ A        VN ++V +++ A   P
Sbjct: 132 GLENEA-NCHCSTHVNPKIVHKLSYATPDP 159

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.8e-09
Identity = 36/126 (28.57%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           KA + +  ++ V  RLKLL+ KK  + ++ ++++   +  G D+ A  RVE I++++  V
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARC-GDLPELQHIRKLFGE 129
            A EIL+ +C+ +L     I+  K+  + + E+VS+L++AA R   ++ EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRF 135
           +Y + +
Sbjct: 127 KYSKEY 132

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.8e-09
Identity = 36/126 (28.57%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           KA + +  ++ V  RLKLL+ KK  + ++ ++++   +  G D+ A  RVE I++++  V
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARC-GDLPELQHIRKLFGE 129
            A EIL+ +C+ +L     I+  K+  + + E+VS+L++AA R   ++ EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRF 135
           +Y + +
Sbjct: 127 KYSKEY 132

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.8e-09
Identity = 36/126 (28.57%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           KA + +  ++ V  RLKLL+ KK  + ++ ++++   +  G D+ A  RVE I++++  V
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARC-GDLPELQHIRKLFGE 129
            A EIL+ +C+ +L     I+  K+  + + E+VS+L++AA R   ++ EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRF 135
           +Y + +
Sbjct: 127 KYSKEY 132

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.8e-09
Identity = 36/126 (28.57%), Postives = 73/126 (57.94%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           KA + +  ++ V  RLKLL+ KK  + ++ ++++   +  G D+ A  RVE I++++  V
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARC-GDLPELQHIRKLFGE 129
            A EIL+ +C+ +L     I+  K+  + + E+VS+L++AA R   ++ EL+ +      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 130 RYGRRF 135
           +Y + +
Sbjct: 127 KYSKEY 132

[Close]

BLAST of Cp4.1LG05g14930 vs. NCBI nr
Match: XP_023534324.1 (uncharacterized protein LOC111795916 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 801 bits (2070), Expect = 1.84e-291
Identity = 416/460 (90.43%), Postives = 416/460 (90.43%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFESAAVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT
Sbjct: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. NCBI nr
Match: KAG6604805.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 793 bits (2048), Expect = 4.13e-288
Identity = 412/460 (89.57%), Postives = 413/460 (89.78%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFESAAVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQH+SNQTDTD TESRTDIEN SSKISVKGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDATESRTDIENPSSKISVKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMP ERPKESQKVNFCRT
Sbjct: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. NCBI nr
Match: XP_022970710.1 (uncharacterized protein LOC111469611 [Cucurbita maxima])

HSP 1 Score: 792 bits (2045), Expect = 1.18e-287
Identity = 411/460 (89.35%), Postives = 412/460 (89.57%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFESAAVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINPVSDDDKQRMMNEIARDCLQPE 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQH SNQTDTDPTESRTDIEN SSKISVKGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHRSNQTDTDPTESRTDIENPSSKISVKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKG+NFTTVQNGSEGATDKEMEWASFYKKPGRRRTKR ELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGENFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRRELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMP ERPKESQKVNFCRT
Sbjct: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. NCBI nr
Match: KAG7034924.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 788 bits (2035), Expect = 3.95e-286
Identity = 409/460 (88.91%), Postives = 412/460 (89.57%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKH+DCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFESAAVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFP+ERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPDERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQH+SNQTDTD TESRTDIEN SSKISVKGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDATESRTDIENPSSKISVKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKK AANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMP ERPKESQKVNFCRT
Sbjct: 361 TYNGNKKDAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. NCBI nr
Match: XP_022947779.1 (uncharacterized protein LOC111451538 [Cucurbita moschata])

HSP 1 Score: 785 bits (2027), Expect = 6.54e-285
Identity = 406/460 (88.26%), Postives = 412/460 (89.57%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAF+RVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFSRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKH+DCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFES+AVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESSAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQH+SNQTDTD TESRTDIEN SSKIS+KGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDRTESRTDIENPSSKISIKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKKVAANDTKSNAMENCEEMKL SSLRNKREALYLRAETMP ERPKESQ+VNFCRT
Sbjct: 361 TYNGNKKVAANDTKSNAMENCEEMKLPSSLRNKREALYLRAETMPPERPKESQRVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy TrEMBL
Match: A0A6J1I3N6 (uncharacterized protein LOC111469611 OS=Cucurbita maxima OX=3661 GN=LOC111469611 PE=3 SV=1)

HSP 1 Score: 792 bits (2045), Expect = 5.73e-288
Identity = 411/460 (89.35%), Postives = 412/460 (89.57%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFESAAVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINPVSDDDKQRMMNEIARDCLQPE 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQH SNQTDTDPTESRTDIEN SSKISVKGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHRSNQTDTDPTESRTDIENPSSKISVKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKG+NFTTVQNGSEGATDKEMEWASFYKKPGRRRTKR ELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGENFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRRELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMP ERPKESQKVNFCRT
Sbjct: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPPERPKESQKVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy TrEMBL
Match: A0A6J1G7W6 (uncharacterized protein LOC111451538 OS=Cucurbita moschata OX=3662 GN=LOC111451538 PE=3 SV=1)

HSP 1 Score: 785 bits (2027), Expect = 3.17e-285
Identity = 406/460 (88.26%), Postives = 412/460 (89.57%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAF+RVE
Sbjct: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFSRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDENRVAAYEILDNFCEFILLNLSYIRKH+DCPNDVNEAVSSLMFAAARCGDLPELQ
Sbjct: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQ------------------------------ 180
           HIRKLFGERYGRRFES+AVQLGPGNLVNCQ                              
Sbjct: 121 HIRKLFGERYGRRFESSAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQ 180

Query: 181 --------------VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240
                         VVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE
Sbjct: 181 LLALQYRSDWHQNQVVARIAKADEKPDQHINQANSGTSSSCDSLPQFPEERIVYLDDVVE 240

Query: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMG 300
           LCSSTNIEGDQRLFKFKTAATLPKTEITQH+SNQTDTD TESRTDIEN SSKIS+KGSMG
Sbjct: 241 LCSSTNIEGDQRLFKFKTAATLPKTEITQHNSNQTDTDRTESRTDIENPSSKISIKGSMG 300

Query: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360
           TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF
Sbjct: 301 TEGKHQIKGKNFTTVQNGSEGATDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVF 360

Query: 361 TYNGNKKVAANDTKSNAMENCEEMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRT 416
           TYNGNKKVAANDTKSNAMENCEEMKL SSLRNKREALYLRAETMP ERPKESQ+VNFCRT
Sbjct: 361 TYNGNKKVAANDTKSNAMENCEEMKLPSSLRNKREALYLRAETMPPERPKESQRVNFCRT 420

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy TrEMBL
Match: A0A6J1CEV2 (IST1-like protein isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 483 bits (1244), Expect = 1.29e-165
Identity = 286/498 (57.43%), Postives = 332/498 (66.67%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MF   FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDV++LI+NGY+QTAFNRVE
Sbjct: 1   MFDFLFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           QIVKDE+R+ AYEIL NFCEFIL NLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ
Sbjct: 61  QIVKDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 120

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQVVARI-AKADEKPDQH-------------- 180
            IRKLFGERYGRRFE++AV+L PGNLVN Q+  ++ A +  + D+H              
Sbjct: 121 LIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQ 180

Query: 181 ------------------INQA-----------------------NSGTSSSCDSLPQFP 240
                             I QA                        S  S+SCD+LPQFP
Sbjct: 181 LLALEYRSDWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFP 240

Query: 241 EERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHSSNQTDTDPTESRTDIE 300
           EERIV++DDVVELCSST  EGDQ LFKFKTAA  P+ +   +HSSNQ   DP++S ++IE
Sbjct: 241 EERIVHVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIE 300

Query: 301 NSSSKISVKGS--------MGTEGKHQIKGKNFTTVQNG---------SEGATDKEMEWA 360
           NSSSK S KGS        M     +  KG N   +            SEGATDKEMEWA
Sbjct: 301 NSSSKGSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWA 360

Query: 361 SFYKKPGRRRTKRS---ELPSSSDLSSTTYDVFTYNG-----NKKVAANDTKSNAMENCE 415
           SFYKKP RRR ++     LP S DL S  YDVFTY G       K      KSNA++N +
Sbjct: 361 SFYKKPRRRRRRKPPPLRLPCS-DLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGD 420

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy TrEMBL
Match: A0A6J1CHT9 (IST1-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 5.95e-163
Identity = 286/504 (56.75%), Postives = 332/504 (65.87%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MF   FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDV++LI+NGY+QTAFNRVE
Sbjct: 1   MFDFLFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVE 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHK------DCPNDVNEAVSSLMFAAARCG 120
           QIVKDE+R+ AYEIL NFCEFIL NLSYIRKHK      DCPNDVNEAVSSLMFA+ARCG
Sbjct: 61  QIVKDESRMVAYEILGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCG 120

Query: 121 DLPELQHIRKLFGERYGRRFESAAVQLGPGNLVNCQVVARI-AKADEKPDQH-------- 180
           DLPELQ IRKLFGERYGRRFE++AV+L PGNLVN Q+  ++ A +  + D+H        
Sbjct: 121 DLPELQLIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIAR 180

Query: 181 ------------------------INQA-----------------------NSGTSSSCD 240
                                   I QA                        S  S+SCD
Sbjct: 181 DCFQPQLLALEYRSDWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCD 240

Query: 241 SLPQFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHSSNQTDTDPTE 300
           +LPQFPEERIV++DDVVELCSST  EGDQ LFKFKTAA  P+ +   +HSSNQ   DP++
Sbjct: 241 TLPQFPEERIVHVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSD 300

Query: 301 SRTDIENSSSKISVKGS--------MGTEGKHQIKGKNFTTVQNG---------SEGATD 360
           S ++IENSSSK S KGS        M     +  KG N   +            SEGATD
Sbjct: 301 SWSEIENSSSKGSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATD 360

Query: 361 KEMEWASFYKKPGRRRTKRS---ELPSSSDLSSTTYDVFTYNG-----NKKVAANDTKSN 415
           KEMEWASFYKKP RRR ++     LP S DL S  YDVFTY G       K      KSN
Sbjct: 361 KEMEWASFYKKPRRRRRRKPPPLRLPCS-DLKSAAYDVFTYPGYGSNTETKKLKETRKSN 420

[Close]

BLAST of Cp4.1LG05g14930 vs. ExPASy TrEMBL
Match: A0A6J1CFH7 (uncharacterized protein LOC111011049 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 466 bits (1199), Expect = 9.39e-159
Identity = 283/504 (56.15%), Postives = 329/504 (65.28%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MF   FGWRKASKCKKLIKQVQCRLKLLKNKK +I KQ++EDV++LI+NGY+QTAFNR  
Sbjct: 1   MFDFLFGWRKASKCKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNR-- 60

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHK------DCPNDVNEAVSSLMFAAARCG 120
            IVKDE+R+ AYEIL NFCEFIL NLSYIRKHK      DCPNDVNEAVSSLMFA+ARCG
Sbjct: 61  -IVKDESRMVAYEILGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCG 120

Query: 121 DLPELQHIRKLFGERYGRRFESAAVQLGPGNLVNCQVVARI-AKADEKPDQH-------- 180
           DLPELQ IRKLFGERYGRRFE++AV+L PGNLVN Q+  ++ A +  + D+H        
Sbjct: 121 DLPELQLIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIAR 180

Query: 181 ------------------------INQA-----------------------NSGTSSSCD 240
                                   I QA                        S  S+SCD
Sbjct: 181 DCFQPQLLALEYRSDWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCD 240

Query: 241 SLPQFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHSSNQTDTDPTE 300
           +LPQFPEERIV++DDVVELCSST  EGDQ LFKFKTAA  P+ +   +HSSNQ   DP++
Sbjct: 241 TLPQFPEERIVHVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSD 300

Query: 301 SRTDIENSSSKISVKGS--------MGTEGKHQIKGKNFTTVQNG---------SEGATD 360
           S ++IENSSSK S KGS        M     +  KG N   +            SEGATD
Sbjct: 301 SWSEIENSSSKGSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATD 360

Query: 361 KEMEWASFYKKPGRRRTKRS---ELPSSSDLSSTTYDVFTYNG-----NKKVAANDTKSN 415
           KEMEWASFYKKP RRR ++     LP S DL S  YDVFTY G       K      KSN
Sbjct: 361 KEMEWASFYKKPRRRRRRKPPPLRLPCS-DLKSAAYDVFTYPGYGSNTETKKLKETRKSN 420

[Close]

BLAST of Cp4.1LG05g14930 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 195.7 bits (496), Expect = 7.6e-50
Identity = 142/432 (32.87%), Postives = 211/432 (48.84%), Query Frame = 0


Query: 1   MFGIFFGWRKASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVE 60
           MF   FGWR+ SKCK ++KQ+QCRL LLKNKK  I+  ++ D+ QL+R G    A +R +
Sbjct: 71  MFCFLFGWRRTSKCKSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQ 130

Query: 61  QIVKDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQ 120
           Q+  DEN ++ Y +L +F + ILLNLSYIR+ +D P+ +NEAVS+L+FA+ARCGDLPEL+
Sbjct: 131 QLFLDENLMSLYHLLLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELR 190

Query: 121 HIRKLFGERYGRRFESAAVQLGPGNLVNCQVVAR---IAKADEKPDQHINQANSGTSSSC 180
            +R LFG+RYG  F   A+ L PGN VN QV+ +   I+ +D+   + + +     +   
Sbjct: 191 ALRVLFGKRYGNHFVDTALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLRL 250

Query: 181 DSL-----PQF-----------PEERIVYLDDVVELCSSTNIEGDQRLFKFK-TAATLPK 240
           + L     P+F            E+ ++  +     CSS   E +  ++KF  T A + +
Sbjct: 251 EVLAIEYTPEFHKQVLKSEKAEEEKEVMSSNSAQPYCSSQKAESEAEVYKFTLTDADIEE 310

Query: 241 TEITQHSSNQTDTDPTESRTDIENSSSKISVKGSMGTEGKH--QIKGKNFTTVQNGSEGA 300
            +  +      + D  E    +E   S    + S   E K   +++ K  +T  + S  A
Sbjct: 311 NQEQERKVCDEEDDCIEEEV-VEEDQSVFRFRESTEDERKERKRLRRKPRSTSSSSSPIA 370

Query: 301 TDKEMEWASFYKKPGRRRTKRSELPSSSDLSSTTYDVFTYNGNKKVAANDTKSNAMENCE 360
            D +  W  +YK  G+R  +R E           Y+VFT                     
Sbjct: 371 KDVDC-WRYYYK--GKRSRQRKE--CGKCCYHIVYNVFT--------------------- 430

Query: 361 EMKLSSSLRNKREALYLRAETMPTERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDDIA 411
                                +P ++  E  + +F        KK  HVHPKLPDYD I 
Sbjct: 431 --------------------MLPDQKESEEGERSF--------KKAMHVHPKLPDYDQIV 447

[Close]

BLAST of Cp4.1LG05g14930 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 137.1 bits (344), Expect = 3.2e-32
Identity = 120/438 (27.40%), Postives = 201/438 (45.89%), Query Frame = 0


Query: 11  ASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRVA 70
           +SKCK   K    R+KL++NK+ ++ KQM+ D+  L+++G D TA  RVE +++++N  A
Sbjct: 28  SSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQA 87

Query: 71  AYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIRKLFGERY 130
           A EI++ FCE I+  L+ I K K CP D+ E ++SL+FAA RC ++PEL  +R +F ++Y
Sbjct: 88  ANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKY 147

Query: 131 GRRFESAAVQLGPGNLVNCQVVARIAKADEKPDQHIN---------QANSGTSSSCDSLP 190
           G+ F SAA  L P   VN  ++ +++  +   +  +          Q +  T+ +   L 
Sbjct: 148 GKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELL 207

Query: 191 QFPEERIVYLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEITQHSSNQTDTDPTESRTD 250
           +  EE I   D   +  S++++  ++         T      T   S  T    TES  +
Sbjct: 208 KPQEESI---DGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAE 267

Query: 251 IENSSSKISVKGSMGTE---GKHQIKGKNFTTVQNGSEGATD-----------------K 310
                +K +V  +        +     K F+   + S    D                 +
Sbjct: 268 AATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSR 327

Query: 311 EMEWASFYKKPG--RRRTKRSELPSSSDLSSTTYDVFTYNGNKKVAANDTKSNAMENCEE 370
           + E +S+Y KPG   R   R    ++  ++ + Y+   Y   +  A    +     N   
Sbjct: 328 DSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEE-EYTNTEAEAKETMRRRHSYNPRS 387

Query: 371 MKLSSSLRNK-REALYLRAETMPTERPKESQKVNFCRTNSCPDKKPT-----------HV 406
           +   ++   K  E+ Y   ET P E P +  +V+    N  P + P             V
Sbjct: 388 VPPPATSEIKFDESDYYEEETEPDE-PSQG-RVSSLPPNRAPPQAPQSGESRQDSSGHQV 447

[Close]

BLAST of Cp4.1LG05g14930 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-29
Identity = 65/154 (42.21%), Postives = 102/154 (66.23%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           K +KCK  ++    RLK+LKNKK I  KQ++ ++ QL+ +G   TA  RVE +V++E  V
Sbjct: 10  KPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTV 69

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIRKLFGER 129
           AAYE++  +CE +++ L  I   K+CP D+ EAV+S++FA+ R  D+PEL  I K F  +
Sbjct: 70  AAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTK 129

Query: 130 YGRRFESAAVQLGPGNLVNCQVVARI-AKADEKP 163
           YG+ F ++AV+L P + V+  +V ++ AKA + P
Sbjct: 130 YGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGP 163

[Close]

BLAST of Cp4.1LG05g14930 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.5e-29
Identity = 64/154 (41.56%), Postives = 100/154 (64.94%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           KA+KCK L+K    R+KL++N++    KQM+ ++ +L+  G + TA  RVE I+++E  +
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIRKLFGER 129
           AA EIL+ FCE I + L  I   ++CP D+ EA+SS+ FAA RC DL ELQ ++ LF  +
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 130 YGRRFESAAVQLGPGNLVNCQVVARIAKADEKPD 164
           YG+ F +AA +L P + VN ++V  ++     P+
Sbjct: 133 YGKEFVAAASELKPDSGVNRKLVELLSVRAPSPE 166

[Close]

BLAST of Cp4.1LG05g14930 vs. TAIR 10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 126.3 bits (316), Expect = 5.6e-29
Identity = 62/139 (44.60%), Postives = 93/139 (66.91%), Query Frame = 0


Query: 10  KASKCKKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIVKDENRV 69
           KA+KCK L+K    R+KL++N++    KQM+ ++ +L+  G + TA  RVE I+++E  +
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 70  AAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIRKLFGER 129
           AA EIL+ FCE I + L  I   ++CP D+ EA+SS+ FAA RC DL ELQ ++ LF  +
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 130 YGRRFESAAVQLGPGNLVN 149
           YG+ F +AA +L P + VN
Sbjct: 133 YGKEFVAAASELKPDSGVN 151

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g14930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54I39	2.1e-12	28.00	IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1	[more]
Q3ZBV1	4.8e-09	28.57	IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1	[more]
P53990	4.8e-09	28.57	IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1	[more]
Q9CX00	4.8e-09	28.57	IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1	[more]
Q568Z6	4.8e-09	28.57	IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1	[more]

BLAST of Cp4.1LG05g14930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023534324.1	1.84e-291	90.43	uncharacterized protein LOC111795916 [Cucurbita pepo subsp. pepo]	[more]
KAG6604805.1	4.13e-288	89.57	IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022970710.1	1.18e-287	89.35	uncharacterized protein LOC111469611 [Cucurbita maxima]	[more]
KAG7034924.1	3.95e-286	88.91	IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022947779.1	6.54e-285	88.26	uncharacterized protein LOC111451538 [Cucurbita moschata]	[more]

BLAST of Cp4.1LG05g14930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I3N6	5.73e-288	89.35	uncharacterized protein LOC111469611 OS=Cucurbita maxima OX=3661 GN=LOC111469611...	[more]
A0A6J1G7W6	3.17e-285	88.26	uncharacterized protein LOC111451538 OS=Cucurbita moschata OX=3662 GN=LOC1114515...	[more]
A0A6J1CEV2	1.29e-165	57.43	IST1-like protein isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3...	[more]
A0A6J1CHT9	5.95e-163	56.75	IST1-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3...	[more]
A0A6J1CFH7	9.39e-159	56.15	uncharacterized protein LOC111011049 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Cp4.1LG05g14930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14830.1	7.6e-50	32.87	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G35730.1	3.2e-32	27.40	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT2G19710.1	1.1e-29	42.21	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.2	1.5e-29	41.56	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.1	5.6e-29	44.60	Regulator of Vps4 activity in the MVB pathway protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 11..31
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 221..235
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 221..261
None	No IPR available	PANTHER	PTHR12161:SF44	IST1P	coord: 7..155 coord: 163..410
IPR042277	Vacuolar protein sorting-associated protein IST1-like	GENE3D	1.20.1260.60		coord: 4..169 e-value: 3.8E-51 score: 175.2
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 15..158 e-value: 1.1E-45 score: 155.5
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 7..155
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 163..410

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g14930.1	Cp4.1LG05g14930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport