Cp4.1LG05g13770 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g13770
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionsubtilisin-like protease SBT1.6
LocationCp4.1LG05: 9709271 .. 9711595 (-)
RNA-Seq ExpressionCp4.1LG05g13770
SyntenyCp4.1LG05g13770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTTGTTTCGCCGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTACTCCTGTTAATCCATGGCTCTACAGTTCCGGCTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCGCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGGTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCTGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTGGCCCCTAAGGCACGTTTGGCAGTTTACAAAGTCTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCTGCGTTTGATTCTGCTGTTAACGACGGCGTCGATGTCATTTCGATCTCGATTGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTGACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACAATTGACCGGAATTTCCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCGCTGTACGCCGGAGCGCCATTGAACGGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCGCTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGCGACAGAGGAAGTAGCCCCAGAGTGGCGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTCTGGCGAATGGAATTTCGAACGGTGAAGGACTCGTCGGCGATGCCCATATCCTTCCCGCCTGCGCCGTCGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACACGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCAAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCCGCTTGGACAGACGTCGTCGGCCCAACTGGTTTAGACTTCGATACACGAAAAACAGAGTTCAATATCTTGTCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCTGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCAGTAAGATCCGCCATGATGACCACCGCAAGAATCACCGACAACCGACGGCAGCCGACGACGGAAGAATCCACCGGAAAAGCTTCTACGCCGTACGATTTCGGTGCCGGTCATGTGAATCTCGGCCTTGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACAGATCAAAGATGATCCAGGTCATATCTCGAACGCCGGTGAAATGTCCAACGAGGAAGCCATTGCCAGGGAATTTGAACTACCCATCAATCGTAGCAGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATCCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCGACGGTGAAGGTAAAACCATCGAAACTAGTATTCTCAACGGCAGTAAAGAAACAGAGCTTCGTAGTGGCCATATCAGCTAACAACCAGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTTATGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACCAAATTAGAGCCGTTGTAA

mRNA sequence

ATGGCCGCTTGTTTCGCCGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTACTCCTGTTAATCCATGGCTCTACAGTTCCGGCTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCGCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGGTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCTGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTGGCCCCTAAGGCACGTTTGGCAGTTTACAAAGTCTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCTGCGTTTGATTCTGCTGTTAACGACGGCGTCGATGTCATTTCGATCTCGATTGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTGACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACAATTGACCGGAATTTCCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCGCTGTACGCCGGAGCGCCATTGAACGGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCGCTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGCGACAGAGGAAGTAGCCCCAGAGTGGCGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTCTGGCGAATGGAATTTCGAACGGTGAAGGACTCGTCGGCGATGCCCATATCCTTCCCGCCTGCGCCGTCGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACACGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCAAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCCGCTTGGACAGACGTCGTCGGCCCAACTGGTTTAGACTTCGATACACGAAAAACAGAGTTCAATATCTTGTCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCTGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCAGTAAGATCCGCCATGATGACCACCGCAAGAATCACCGACAACCGACGGCAGCCGACGACGGAAGAATCCACCGGAAAAGCTTCTACGCCGTACGATTTCGGTGCCGGTCATGTGAATCTCGGCCTTGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACAGATCAAAGATGATCCAGGTCATATCTCGAACGCCGGTGAAATGTCCAACGAGGAAGCCATTGCCAGGGAATTTGAACTACCCATCAATCGTAGCAGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATCCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCGACGGTGAAGGTAAAACCATCGAAACTAGTATTCTCAACGGCAGTAAAGAAACAGAGCTTCGTAGTGGCCATATCAGCTAACAACCAGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTTATGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACCAAATTAGAGCCGTTGTAA

Coding sequence (CDS)

ATGGCCGCTTGTTTCGCCGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTACTCCTGTTAATCCATGGCTCTACAGTTCCGGCTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCGCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGGTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCTGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTGGCCCCTAAGGCACGTTTGGCAGTTTACAAAGTCTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCTGCGTTTGATTCTGCTGTTAACGACGGCGTCGATGTCATTTCGATCTCGATTGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTGACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACAATTGACCGGAATTTCCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCGCTGTACGCCGGAGCGCCATTGAACGGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCGCTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGCGACAGAGGAAGTAGCCCCAGAGTGGCGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTCTGGCGAATGGAATTTCGAACGGTGAAGGACTCGTCGGCGATGCCCATATCCTTCCCGCCTGCGCCGTCGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACACGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCAAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCCGCTTGGACAGACGTCGTCGGCCCAACTGGTTTAGACTTCGATACACGAAAAACAGAGTTCAATATCTTGTCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCTGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCAGTAAGATCCGCCATGATGACCACCGCAAGAATCACCGACAACCGACGGCAGCCGACGACGGAAGAATCCACCGGAAAAGCTTCTACGCCGTACGATTTCGGTGCCGGTCATGTGAATCTCGGCCTTGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACAGATCAAAGATGATCCAGGTCATATCTCGAACGCCGGTGAAATGTCCAACGAGGAAGCCATTGCCAGGGAATTTGAACTACCCATCAATCGTAGCAGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATCCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCGACGGTGAAGGTAAAACCATCGAAACTAGTATTCTCAACGGCAGTAAAGAAACAGAGCTTCGTAGTGGCCATATCAGCTAACAACCAGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTTATGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACCAAATTAGAGCCGTTGTAA

Protein sequence

MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFAESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL
Homology
[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 558/767 (72.75%), Postives = 659/767 (85.92%), Query Frame = 0 

Query: 10  SSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +  A    KTFIFR+D  + PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TV HGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAG 429
           G PLNG M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRVAKGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RKTEFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAHPDWSPA +RSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCP-TRKPLPGNLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGY  K IQVI+RTPV+CP TRKP PGNLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQ 729
           SI AVF +  +GL +K+ IRTATNVG A +VY+A+IE+P+G TV VKP +LVF++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANNQNLTLGDVGAVFGSLLWSD-GKHVVRTPLVVTKLEPL 775
           S+ V ++ N +N+ LG+ GAVFGS+ W D GKHVVR+P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 4.8e-244
Identity = 446/779 (57.25%), Postives = 547/779 (70.22%), Query Frame = 0 

Query: 14  FFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA----ESHKILHVY 73
           FF LL L   S+  +     T+I  VD  AKPS+FPTHFHWYTS  A        I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 74  DTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSD 133
           DTV HGFSA  T + A  +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 134 YGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSK 193
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 194 GHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 253
           G+EA          +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 254 PKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGA 313
           PKARLA YKVCW NSGC+DSDILAAFD+AV DGVDVIS+S+G   GV  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGA 306

Query: 314 YGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSL 373
           +GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 374 YAGAPLN-GTMYPLVYPGK---SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGL 433
           Y G  L+ G MYPLVY G        S+SLC+E SLDP +V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 434 VVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYAS------SSGNPTA 493
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 494 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 553
           TI F+GTR+GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 554 TRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEES 613
            R+TEFNILSGTSMACPH+SG AALLK+AHPDWSPAA+RSA++TTA   DN  +P  +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 614 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKC-- 673
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 674 PTRKPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVK 733
             R    GNLNYPS   VF    +   +  FIRT TNVG ++SVY+ KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 734 PSKLVFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 775
           P KL F    +K SFVV +      L+ G      G ++WSDGK  V +PLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.6e-207
Identity = 391/770 (50.78%), Postives = 523/770 (67.92%), Query Frame = 0 

Query: 9   LSSSIFFSLLLL--IHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTS---EFAESH 68
           LSS+ FF LL L   H S+  +D    T+I  +     PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQ--GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+  +HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNGTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R+ 
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS HP+WSPAA+RSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKC-PTRKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y S  I+ +SR    C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEA-PKGATVKVKPSKL 728
              +LNYPS    F+    G+    + RT T+VG A + Y  K+ +   G  + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 VFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVT 770
            F  A +K+S+ V  + ++   +  +    FGS+ WSDGKHVV +P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 4.8e-204
Identity = 380/769 (49.41%), Postives = 506/769 (65.80%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MA+  ++S S +I  + L L+  +T       KT+I RV+   KP  F TH  WYTS+  
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLN 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADS-VGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 120
               +L+ Y T  HGFSA     +ADS +    S+L +FED    LHTTR+P+FLGL ++
Sbjct: 61  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 120

Query: 121 RGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKI 180
            G+       + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K+
Sbjct: 121 FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 180

Query: 181 VGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYA 240
           +GAR FSKG +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA
Sbjct: 181 IGARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYY 300
           +G A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S PYY
Sbjct: 241 AGTARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYY 300

Query: 301 LDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNR 360
            D IAIGA+ A  RG+FVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   +GN 
Sbjct: 301 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 360

Query: 361 RKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 420
           +++ GVSLY+G  +      LVY  K    S++LC+  SLD  IV GKIV+CDRG + RV
Sbjct: 361 KRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARV 420

Query: 421 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 480
            KG VV+ AGG+GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S   PTA +
Sbjct: 421 EKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALL 480

Query: 481 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 540
            F+GT + +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+D +GPTGLD D+R
Sbjct: 481 VFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR 540

Query: 541 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 600
           +T+FNI+SGTSM+CPHISG A LLK+AHP+WSP+A++SA+MTTA + DN   P  + +  
Sbjct: 541 RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADN 600

Query: 601 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYR-SKMIQVISRTPVKCPTR 660
             S PY  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    ++ ++ R  V C  +
Sbjct: 601 SLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 660

Query: 661 KPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSK 720
              PG LNYPS    FS L  G     + R  TNVGAA+SVY+  +       + VKPSK
Sbjct: 661 FSDPGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 720

Query: 721 LVF-STAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPL 767
           L F S   KK+  V  +S    ++T     A FGS+ WS+ +H VR+P+
Sbjct: 721 LSFKSVGEKKRYTVTFVSKKGVSMT---NKAEFGSITWSNPQHEVRSPV 747

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 2.2e-196
Identity = 374/777 (48.13%), Postives = 497/777 (63.96%), Query Frame = 0 

Query: 18  LLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D  A P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T  HG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GG  GVST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGG--GVST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NGTMYPLVYPGK--SGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +      YPLVY G+  S     S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 497
            KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S   TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 617
           + +FNILSGTSM+CPH+SG AAL+KS HPDWSPAA++SA+MTTA + DN  +P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKC--PT 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V ++   +    T
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 RKPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPS 737
               PGNLNYP+I A+F   +  +   +  RT TNVG   S Y+  +   KGA+V V+P 
Sbjct: 680 LAKNPGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 739

Query: 738 KLVFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 775
            L F++  +K S+ V      +          FG L+W    H VR+P+++T L PL
Sbjct: 740 TLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

[Close]
BLAST of Cp4.1LG05g13770 vs. NCBI nr
Match: XP_023532451.1 (subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1522 bits (3940), Expect = 0.0
Identity = 774/774 (100.00%), Postives = 774/774 (100.00%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV
Sbjct: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. NCBI nr
Match: KAG6604929.1 (Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027008.1 Subtilisin-like protease SBT1.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1498 bits (3877), Expect = 0.0
Identity = 762/774 (98.45%), Postives = 765/774 (98.84%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAACFAASLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVDSF+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRTPVKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FSTAVKKQSFVVAISAN+QNLTLGDVGAVFGSL WSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. NCBI nr
Match: XP_022946948.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1487 bits (3850), Expect = 0.0
Identity = 758/774 (97.93%), Postives = 760/774 (98.19%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAACF ASLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVDSF+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRT VKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKL 
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FSTAVKKQSFVVAISAN  NLTLGDVGAVFGSL WSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. NCBI nr
Match: XP_022970938.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1480 bits (3831), Expect = 0.0
Identity = 754/774 (97.42%), Postives = 759/774 (98.06%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAACFA SLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVD FAKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
            EFNILSGTSMACPHISGAAALLKSAH DWSPAA+RSAMMTTA+ITDNR QPTTEESTGK
Sbjct: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRTPVKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LPGNLNYPSIVAVFSSLSK LSTKSFIRTATNVGAANSVYQAKIEAPKG TVKVKPSKLV
Sbjct: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FSTAVKKQSFVVAISANNQNLTLG+VGAVFGSL WSDGKHVVR PLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. NCBI nr
Match: XP_038901136.1 (subtilisin-like protease SBT1.6 [Benincasa hispida])

HSP 1 Score: 1384 bits (3582), Expect = 0.0
Identity = 702/774 (90.70%), Postives = 730/774 (94.32%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAA  + SLS   FF LLLL   STV AD PLKTFIFRVD F+KPSVFPTH+HWYTSEFA
Sbjct: 1   MAAFLSTSLSP--FFFLLLLTLHSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ES KILHVYDTV HGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESPKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NAAGPIIGINDT+EYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R
Sbjct: 301 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KI GVSLYAGAPLNGTMYPLVYPGKSG LS SLCME+SLDPK V GKIVICDRGSSPRVA
Sbjct: 361 KISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NP ATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPIATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA ITDNRRQP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGY  KMIQVI+RTPVKCP +KP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVKCPAKKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIV+VFSSLSKG STKSFIRT TNVG +NS Y+AKIEAPKG TVKVKPSKLV
Sbjct: 661 LPENLNYPSIVSVFSSLSKGWSTKSFIRTVTNVGPSNSAYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FST VKKQSFVVAISA+NQNL LGDVGAVFG L WSDGKHVVR+PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 772

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy TrEMBL
Match: A0A6J1G574 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111450975 PE=3 SV=1)

HSP 1 Score: 1487 bits (3850), Expect = 0.0
Identity = 758/774 (97.93%), Postives = 760/774 (98.19%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAACF ASLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVDSF+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRT VKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKL 
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FSTAVKKQSFVVAISAN  NLTLGDVGAVFGSL WSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy TrEMBL
Match: A0A6J1I5D0 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111469756 PE=3 SV=1)

HSP 1 Score: 1480 bits (3831), Expect = 0.0
Identity = 754/774 (97.42%), Postives = 759/774 (98.06%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAACFA SLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVD FAKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
            EFNILSGTSMACPHISGAAALLKSAH DWSPAA+RSAMMTTA+ITDNR QPTTEESTGK
Sbjct: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRTPVKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LPGNLNYPSIVAVFSSLSK LSTKSFIRTATNVGAANSVYQAKIEAPKG TVKVKPSKLV
Sbjct: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FSTAVKKQSFVVAISANNQNLTLG+VGAVFGSL WSDGKHVVR PLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1373 bits (3555), Expect = 0.0
Identity = 689/769 (89.60%), Postives = 727/769 (94.54%), Query Frame = 0 

Query: 7   ASLSSSIFFSLLLLIHGSTVPADPPLK-TFIFRVDSFAKPSVFPTHFHWYTSEFAESHKI 66
           A+  S +FF  LL++H  TV AD PLK TFIFRVD F+KPSVFPTH+HWYTSEFAESHKI
Sbjct: 2   AAYLSPLFFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKI 61

Query: 67  LHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD 126
           LHVYDTV HGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD
Sbjct: 62  LHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD 121

Query: 127 SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFF 186
           SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARFF
Sbjct: 122 SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFF 181

Query: 187 SKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKG 246
           SKGHEAG+NAAGPIIGINDT+E+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAKG
Sbjct: 182 SKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKG 241

Query: 247 VAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAI 306
           VAPKARLAVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYLDPIAI
Sbjct: 242 VAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAI 301

Query: 307 GAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGV 366
           GAYGAASRGIFVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R+I GV
Sbjct: 302 GAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGV 361

Query: 367 SLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVV 426
           SLYAGAPLN TM+PLVYPGKSG LS SLCM++SLDPK+V GKIVICDRGSSPRVAKGLVV
Sbjct: 362 SLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVV 421

Query: 427 KKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTR 486
           KKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGD+MKAYASSS NPTATIAF+GT 
Sbjct: 422 KKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGTI 481

Query: 487 IGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNI 546
           IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRKTEFNI
Sbjct: 482 IGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNI 541

Query: 547 LSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPY 606
           LSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA  TDNRRQP TEESTGKASTPY
Sbjct: 542 LSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPY 601

Query: 607 DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKPLPGNL 666
           DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY  KMIQVI+RTPV CP +KPLP NL
Sbjct: 602 DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPENL 661

Query: 667 NYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAV 726
           NYPSIVAVFSSLSKG STKSFIRT TNVG ANSVY+AKIEAPKG TVKVKPSKLVFS A+
Sbjct: 662 NYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAM 721

Query: 727 KKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           KKQSFVVA+SA+NQNL LGDVGAVFG + WSDGKHVVR+PLVVT+LEPL
Sbjct: 722 KKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1373 bits (3555), Expect = 0.0
Identity = 693/774 (89.53%), Postives = 732/774 (94.57%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MAA  + SLSS  FFS LLL+  STV +  PLKTFI R+D F+KPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSLSS-FFFSFLLLL--STVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTV HGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIG+YGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420
           KI+GVSLYAGAPLNGTMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRVA
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA ITDNRRQP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGY  KMIQVI+RTPV+CPT+KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720
           LP NLNYPSIV VFSSLSKG STKSFIRTATNVG +NSVY+ KIEAPKG TVKVKPSKLV
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           FST VKKQSFVVAISA+NQNL LGDVGAVFG L WSDGKHVVR+PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

[Close]
BLAST of Cp4.1LG05g13770 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1373 bits (3555), Expect = 0.0
Identity = 688/762 (90.29%), Postives = 724/762 (95.01%), Query Frame = 0 

Query: 14  FFSLLLLIHGSTVPADPPLK-TFIFRVDSFAKPSVFPTHFHWYTSEFAESHKILHVYDTV 73
           FF  LL++H  TV AD PLK TFIFRVD F+KPSVFPTH+HWYTSEFAESHKILHVYDTV
Sbjct: 13  FFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKILHVYDTV 72

Query: 74  VHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 133
            HGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV
Sbjct: 73  FHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 132

Query: 134 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAG 193
           IIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARFFSKGHEAG
Sbjct: 133 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFFSKGHEAG 192

Query: 194 ANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARL 253
           +NAAGPIIGINDT+E+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAKGVAPKARL
Sbjct: 193 SNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKGVAPKARL 252

Query: 254 AVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAAS 313
           AVYKVCWKNSGCFDSDILAAFD+AVNDGVDVISISIGGGDGVS+PYYLDPIAIGAYGAAS
Sbjct: 253 AVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 312

Query: 314 RGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAP 373
           RGIFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R+I GVSLYAGAP
Sbjct: 313 RGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGVSLYAGAP 372

Query: 374 LNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAGGVG 433
           LNGTM+PLVYPGKSG LS SLCM++SLDPK+V GKIVICDRGSSPRVAKGLVVKKAGGVG
Sbjct: 373 LNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVVKKAGGVG 432

Query: 434 MILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAP 493
           MILANGISNGEGLVGDAH+LPACAVGS EGD+MKAYASSS NPTATIAF+GT IGIKPAP
Sbjct: 433 MILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAFQGTIIGIKPAP 492

Query: 494 VVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMA 553
           VVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRKTEFNILSGTSMA
Sbjct: 493 VVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 552

Query: 554 CPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHV 613
           CPH+SGAAALLKSAHPDWSPAA+RSAMMTTA  TDNRRQP TEESTGKASTPYDFGAGHV
Sbjct: 553 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPYDFGAGHV 612

Query: 614 NLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKPLPGNLNYPSIVA 673
           NLGLAMDPGLVYDITNTDY+NFLCSIGY  KMIQVI+RTPV CP +KPLP NLNYPSIVA
Sbjct: 613 NLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSIVA 672

Query: 674 VFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQSFVV 733
           VFSSLSKG STKSFIRT TNVG ANSVY+AKIEAPKG TVKVKPSKLVFS A KKQSFVV
Sbjct: 673 VFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAATKKQSFVV 732

Query: 734 AISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774
           A+SA+NQNL LGDVGAVFG + WSDGKHVVR+PLVVT+LEPL
Sbjct: 733 AVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

[Close]
BLAST of Cp4.1LG05g13770 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 558/767 (72.75%), Postives = 659/767 (85.92%), Query Frame = 0 

Query: 10  SSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +  A    KTFIFR+D  + PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TV HGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGLVVKKAG 429
           G PLNG M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRVAKGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RKTEFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAHPDWSPA +RSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCP-TRKPLPGNLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGY  K IQVI+RTPV+CP TRKP PGNLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLVFSTAVKKQ 729
           SI AVF +  +GL +K+ IRTATNVG A +VY+A+IE+P+G TV VKP +LVF++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANNQNLTLGDVGAVFGSLLWSD-GKHVVRTPLVVTKLEPL 775
           S+ V ++ N +N+ LG+ GAVFGS+ W D GKHVVR+P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

[Close]
BLAST of Cp4.1LG05g13770 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 845.5 bits (2183), Expect = 3.4e-245
Identity = 446/779 (57.25%), Postives = 547/779 (70.22%), Query Frame = 0 

Query: 14  FFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA----ESHKILHVY 73
           FF LL L   S+  +     T+I  VD  AKPS+FPTHFHWYTS  A        I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 74  DTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSD 133
           DTV HGFSA  T + A  +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 134 YGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSK 193
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 194 GHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 253
           G+EA          +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 254 PKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPIAIGA 313
           PKARLA YKVCW NSGC+DSDILAAFD+AV DGVDVIS+S+G   GV  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGA 306

Query: 314 YGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSL 373
           +GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 374 YAGAPLN-GTMYPLVYPGK---SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAKGL 433
           Y G  L+ G MYPLVY G        S+SLC+E SLDP +V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 434 VVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYAS------SSGNPTA 493
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 494 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 553
           TI F+GTR+GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 554 TRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEES 613
            R+TEFNILSGTSMACPH+SG AALLK+AHPDWSPAA+RSA++TTA   DN  +P  +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 614 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKC-- 673
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 674 PTRKPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVK 733
             R    GNLNYPS   VF    +   +  FIRT TNVG ++SVY+ KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 734 PSKLVFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 775
           P KL F    +K SFVV +      L+ G      G ++WSDGK  V +PLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

[Close]
BLAST of Cp4.1LG05g13770 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 724.2 bits (1868), Expect = 1.1e-208
Identity = 391/770 (50.78%), Postives = 523/770 (67.92%), Query Frame = 0 

Query: 9   LSSSIFFSLLLL--IHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTS---EFAESH 68
           LSS+ FF LL L   H S+  +D    T+I  +     PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQ--GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+  +HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNGTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVAK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R+ 
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS HP+WSPAA+RSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKC-PTRKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y S  I+ +SR    C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEA-PKGATVKVKPSKL 728
              +LNYPS    F+    G+    + RT T+VG A + Y  K+ +   G  + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 VFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVT 770
            F  A +K+S+ V  + ++   +  +    FGS+ WSDGKHVV +P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

[Close]
BLAST of Cp4.1LG05g13770 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 712.6 bits (1838), Expect = 3.4e-205
Identity = 380/769 (49.41%), Postives = 506/769 (65.80%), Query Frame = 0 

Query: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60
           MA+  ++S S +I  + L L+  +T       KT+I RV+   KP  F TH  WYTS+  
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLN 60

Query: 61  ESHKILHVYDTVVHGFSATFTQEQADS-VGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 120
               +L+ Y T  HGFSA     +ADS +    S+L +FED    LHTTR+P+FLGL ++
Sbjct: 61  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 120

Query: 121 RGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKI 180
            G+       + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K+
Sbjct: 121 FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 180

Query: 181 VGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYA 240
           +GAR FSKG +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA
Sbjct: 181 IGARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYY 300
           +G A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S PYY
Sbjct: 241 AGTARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYY 300

Query: 301 LDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNR 360
            D IAIGA+ A  RG+FVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   +GN 
Sbjct: 301 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 360

Query: 361 RKIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 420
           +++ GVSLY+G  +      LVY  K    S++LC+  SLD  IV GKIV+CDRG + RV
Sbjct: 361 KRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARV 420

Query: 421 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 480
            KG VV+ AGG+GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S   PTA +
Sbjct: 421 EKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALL 480

Query: 481 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 540
            F+GT + +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+D +GPTGLD D+R
Sbjct: 481 VFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR 540

Query: 541 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 600
           +T+FNI+SGTSM+CPHISG A LLK+AHP+WSP+A++SA+MTTA + DN   P  + +  
Sbjct: 541 RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADN 600

Query: 601 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYR-SKMIQVISRTPVKCPTR 660
             S PY  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    ++ ++ R  V C  +
Sbjct: 601 SLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 660

Query: 661 KPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSK 720
              PG LNYPS    FS L  G     + R  TNVGAA+SVY+  +       + VKPSK
Sbjct: 661 FSDPGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 720

Query: 721 LVF-STAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPL 767
           L F S   KK+  V  +S    ++T     A FGS+ WS+ +H VR+P+
Sbjct: 721 LSFKSVGEKKRYTVTFVSKKGVSMT---NKAEFGSITWSNPQHEVRSPV 747

[Close]
BLAST of Cp4.1LG05g13770 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 687.2 bits (1772), Expect = 1.5e-197
Identity = 374/777 (48.13%), Postives = 497/777 (63.96%), Query Frame = 0 

Query: 18  LLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D  A P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T  HG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GG  GVST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGG--GVST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NGTMYPLVYPGK--SGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +      YPLVY G+  S     S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 AKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 497
            KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S   TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 617
           + +FNILSGTSM+CPH+SG AAL+KS HPDWSPAA++SA+MTTA + DN  +P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKC--PT 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V ++   +    T
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 RKPLPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPS 737
               PGNLNYP+I A+F   +  +   +  RT TNVG   S Y+  +   KGA+V V+P 
Sbjct: 680 LAKNPGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 739

Query: 738 KLVFSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 775
            L F++  +K S+ V      +          FG L+W    H VR+P+++T L PL
Sbjct: 740 TLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

The following BLAST results are available for this feature:
BLAST of Cp4.1LG05g13770 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O496070.0e+0072.75Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Q9LUM34.8e-24457.25Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
O653511.6e-20750.78Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF64.8e-20449.41Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9FLI42.2e-19648.13Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
BLAST of Cp4.1LG05g13770 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023532451.10.0100.00subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo][more]
KAG6604929.10.098.45Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022946948.10.097.93subtilisin-like protease SBT1.6 [Cucurbita moschata][more]
XP_022970938.10.097.42subtilisin-like protease SBT1.6 [Cucurbita maxima][more]
XP_038901136.10.090.70subtilisin-like protease SBT1.6 [Benincasa hispida][more]
BLAST of Cp4.1LG05g13770 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G5740.097.93subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111450975 PE... [more]
A0A6J1I5D00.097.42subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111469756 PE=3... [more]
A0A6J1JEF50.089.60subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3... [more]
A0A0A0LJ580.089.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1[more]
A0A6J1FYE50.090.29subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE... [more]
BLAST of Cp4.1LG05g13770 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G34980.10.0e+0072.75subtilisin-like serine protease 2 [more]
AT3G14240.13.4e-24557.25Subtilase family protein [more]
AT5G67360.11.1e-20850.78Subtilase family protein [more]
AT2G05920.13.4e-20549.41Subtilase family protein [more]
AT5G51750.11.5e-19748.13subtilase 1.3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 213..226
score: 50.0
coord: 547..563
score: 58.55
coord: 129..148
score: 32.4
IPR003137PA domainPFAMPF02225PAcoord: 378..464
e-value: 1.1E-8
score: 35.1
NoneNo IPR availableGENE3D3.50.30.30coord: 347..487
e-value: 4.7E-177
score: 591.4
NoneNo IPR availableGENE3D2.60.40.2310coord: 635..771
e-value: 2.5E-38
score: 132.7
NoneNo IPR availablePANTHERPTHR10795:SF675SUBTILISIN-LIKE PROTEASE SBT1.7coord: 22..768
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 104..618
score: 30.071426
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 353..479
e-value: 7.24903E-38
score: 135.619
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 404..473
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 664..768
e-value: 5.2E-27
score: 94.0
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 29..106
e-value: 2.7E-11
score: 45.8
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 131..631
e-value: 4.7E-177
score: 591.4
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 105..622
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 129..593
e-value: 9.6E-47
score: 160.4
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 48..106
e-value: 7.1E-10
score: 39.4
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 22..768
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 548..558
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 104..583
e-value: 1.19663E-143
score: 422.392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g13770.1Cp4.1LG05g13770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity