Cp4.1LG05g13220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG05g13220 vs. ExPASy Swiss-Prot
Match: Q8GX93 (Chloride channel protein CLC-e OS=Arabidopsis thaliana OX=3702 GN=CLC-E PE=2 SV=2)

HSP 1 Score: 734.2 bits (1894), Expect = 1.5e-210
Identity = 409/661 (61.88%), Postives = 490/661 (74.13%), Query Frame = 0

Query: 108 EEDEEEEGIPSGIGSS-TIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLR 167
           E D++E G          I S+C VGVLTG+ VVLFNN VH LRDF WDGIP+RGASWLR
Sbjct: 59  ETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLR 118

Query: 168 EMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKA 227
           E P+   W RVILVP  GG +VS LN LRE+         G   S  ++ +A L+PFLK 
Sbjct: 119 EAPIGSNWLRVILVPTIGGLVVSILNQLRESA----GKSTGDSHSSLDRVKAVLRPFLKT 178

Query: 228 VAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAA 287
           VAA VTLGTGNSLGPEGPSV+IG S+ KGV+++F+++ +T  SL+AAGSAAGISSGFNAA
Sbjct: 179 VAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLFNKSPQTGFSLLAAGSAAGISSGFNAA 238

Query: 288 VAGCFFAVESVLWPSPA-DSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFR 347
           VAGCFFAVESVLWPS + DS+ SL NTTSMVILSAV AS VS++GLG EPAFKVP+YDFR
Sbjct: 239 VAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFR 298

Query: 348 SPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAL 407
           SP ELPLYLLLGALCGLVSL+ S+CTS M + VD ++KD G+ +A+FP++GG S G+IAL
Sbjct: 299 SPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIAL 358

Query: 408 AYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLF 467
            YPE+LYWGF+NVD+LLE RPFVK LSA+LL QLV VKI AT+ CRASGLVGGYYAPSLF
Sbjct: 359 VYPEVLYWGFQNVDILLEKRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLF 418

Query: 468 IGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 527
           IG A GMAYGKFIG+AL++ N   + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLL
Sbjct: 419 IGGAAGMAYGKFIGLALAQ-NPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 478

Query: 528 FELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQ 587
           FELTQDYRIVLPLLGAVG+SSW+TSGQ +++ +++TK+    K     QS T   +++D+
Sbjct: 479 FELTQDYRIVLPLLGAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDE 538

Query: 588 SSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIE 647
           SS           N+LCE+ESSLC  DS ++  EL + I VSEAM TR+ TV M T L E
Sbjct: 539 SST----------NNLCEVESSLCLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEE 598

Query: 648 AVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSK---------NGLVVSEVCSLDGEI 707
           A+  ML EKQS ALIVD +N  +GIL L DIQ+ SK           + V+++CS  G  
Sbjct: 599 ALTRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGK 658

Query: 708 CRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMG----YLVGVLDLECIDLTCRILAT 754
           C+VPWT TP MD+L A+T+M    +S V VV   +     + VGVLD ECI LT R LAT
Sbjct: 659 CKVPWTVTPDMDLLAAQTIMNKHELSHVAVVSGSIDAPRIHPVGVLDRECITLTRRALAT 701

BLAST of Cp4.1LG05g13220 vs. ExPASy Swiss-Prot
Match: Q8RXR2 (Chloride channel protein CLC-f OS=Arabidopsis thaliana OX=3702 GN=CLC-F PE=2 SV=2)

HSP 1 Score: 409.8 bits (1052), Expect = 6.5e-113
Identity = 283/700 (40.43%), Postives = 397/700 (56.71%), Query Frame = 0

Query: 46  RFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEED 105
           RFSL  ++  F+     ++    E  +P   SS   FS   G     GI      ++  D
Sbjct: 59  RFSLSGRRLSFKRME--NIRVDRERHNP---SSSSAFS-AAGEEDGGGISNLHSVDDRND 118

Query: 106 NTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWL 165
               DEE  G  +    + ++  C +GV  GI V  FN  VH + ++ W G PN GA+WL
Sbjct: 119 EYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWL 178

Query: 166 REMPVEETWKRVILVPACGGFLVSFLNLLREATD----NDPSTKLGVPVSISNQFRAALQ 225
           R   + +TW R++L+P  GG +V  ++ L E  D    ++ S + G+       F A + 
Sbjct: 179 RLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGL------DFLAGIY 238

Query: 226 PFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISS 285
           P +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + + N   +++L AAG+A+GI+S
Sbjct: 239 PVIKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGAASGIAS 298

Query: 286 GFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPE 345
           GFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS   LG + AF VP 
Sbjct: 299 GFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVSNALLGTQSAFTVPS 358

Query: 346 YDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTG 405
           YD +S +ELPLYL+LG LCG VS+ FS+  ++   + D +   FG+   + P LGG   G
Sbjct: 359 YDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAG 418

Query: 406 LIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYA 465
           +IAL YP ILYWGF NV+ +L +          LLAQL   K++AT+LC+ SGLVGG YA
Sbjct: 419 IIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYA 478

Query: 466 PSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTA 525
           PSL IGAA G  +G   G A    N AI  +   VA PQAY LVGMAATLA +C VPLT+
Sbjct: 479 PSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGMAATLASMCSVPLTS 538

Query: 526 VLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSN 585
           VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S  ++    G+  S+   +   + 
Sbjct: 539 VLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKTE 598

Query: 586 ANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDT 645
              + ++ AD        +L  IE+       D E +  + K  V   M+  YV V   T
Sbjct: 599 GVWRHTDNADS------LELTVIENPDHNSFLDEETILEDLK--VMRVMSKNYVKVSSGT 658

Query: 646 FLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQK---------LSKNGLVVSEVC-- 705
            L EA +++    Q+  ++VD+++ L GIL   DI++         L +N   VS VC  
Sbjct: 659 TLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTILDENTCPVSSVCTK 718

Query: 706 --SLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVK 729
             S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 719 KISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 731

BLAST of Cp4.1LG05g13220 vs. ExPASy Swiss-Prot
Match: A5F0D5 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=clcA PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.5e-32
Identity = 131/437 (29.98%), Postives = 202/437 (46.22%), Query Frame = 0

Query: 123 STIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPA 182
           S +  S  VG+L G+    F  AVH + +   D + +   S+L        W    L+ A
Sbjct: 32  SVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------PLWLAAFLISA 91

Query: 183 CGGFLVSFL--NLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 242
              F+  FL      EA  +      G    +       + P +K       LG+G  LG
Sbjct: 92  FLAFIGYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWRVLP-VKFFGGMGALGSGMVLG 151

Query: 243 PEGPSVDIGTSVGKGVSTVFD-RNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 302
            EGP+V +G +VG+ +S +F  +N  T+ SL+AAG+A G+++ FNA +AG  F +E +  
Sbjct: 152 REGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIEEM-- 211

Query: 303 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 362
                  ++L +  + VI+SAV A+ V +V  G +    +P+YD    S L L+LLLGAL
Sbjct: 212 --RPQFRYTLISVRA-VIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLGAL 271

Query: 363 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 422
            G+  + F+   +       K H++      L   + G   GL+ L  PE+   G   + 
Sbjct: 272 FGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISLIP 331

Query: 423 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 482
            +           A +L  L V +I  T LC  SG  GG +AP L +G   G A+G    
Sbjct: 332 TITNG-----GYGAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFGLIAK 391

Query: 483 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL- 542
           +   E N            P  + + GM A  A   + P+T +LL+ E+T +Y ++LPL 
Sbjct: 392 VWFPELN----------IEPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 440

Query: 543 ---LGAVGVSSWLTSGQ 553
              LGAV + + L  GQ
Sbjct: 452 ITSLGAV-IFAQLLGGQ 440

BLAST of Cp4.1LG05g13220 vs. ExPASy Swiss-Prot
Match: Q9KM62 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=clcA PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 4.3e-32
Identity = 131/437 (29.98%), Postives = 202/437 (46.22%), Query Frame = 0

Query: 123 STIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPA 182
           S +  S  VG+L G+    F  AVH + +   D + +   S+L        W    L+ A
Sbjct: 32  SVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------PLWLAAFLISA 91

Query: 183 CGGFLVSFL--NLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 242
              F+  FL      EA  +      G    +       + P +K       LG+G  LG
Sbjct: 92  FLAFIGYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWRVLP-VKFFGGMGALGSGMVLG 151

Query: 243 PEGPSVDIGTSVGKGVSTVFD-RNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 302
            EGP+V +G +VG+ +S +F  +N  T+ SL+AAG+A G+++ FNA +AG  F +E +  
Sbjct: 152 REGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIEEM-- 211

Query: 303 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 362
                  ++L +  + VI+SAV A+ V +V  G +    +P+YD    S L L+LLLGAL
Sbjct: 212 --RPQFRYTLISVRA-VIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLGAL 271

Query: 363 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 422
            G+  + F+   +       K H++      L   + G   GL+ L  PE+   G   + 
Sbjct: 272 FGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISLIP 331

Query: 423 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 482
            +           A +L  L V +I  T LC  SG  GG +AP L +G   G A+G    
Sbjct: 332 TITNG-----GYGAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFGLIAK 391

Query: 483 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL- 542
           +   E N            P  + + GM A  A   + P+T +LL+ E+T +Y ++LPL 
Sbjct: 392 MWFPELN----------IEPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 440

Query: 543 ---LGAVGVSSWLTSGQ 553
              LGAV + + L  GQ
Sbjct: 452 ITSLGAV-IFAQLLGGQ 440

BLAST of Cp4.1LG05g13220 vs. ExPASy Swiss-Prot
Match: C3LVE3 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain M66-2) OX=579112 GN=clcA PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 4.3e-32
Identity = 131/437 (29.98%), Postives = 202/437 (46.22%), Query Frame = 0

Query: 123 STIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPA 182
           S +  S  VG+L G+    F  AVH + +   D + +   S+L        W    L+ A
Sbjct: 32  SVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------PLWLAAFLISA 91

Query: 183 CGGFLVSFL--NLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 242
              F+  FL      EA  +      G    +       + P +K       LG+G  LG
Sbjct: 92  FLAFIGYFLVHRFAPEAAGSGIPEIEGAMDGMRPVRWWRVLP-VKFFGGMGALGSGMVLG 151

Query: 243 PEGPSVDIGTSVGKGVSTVFD-RNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 302
            EGP+V +G +VG+ +S +F  +N  T+ SL+AAG+A G+++ FNA +AG  F +E +  
Sbjct: 152 REGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAAGGLAAAFNAPLAGIMFVIEEM-- 211

Query: 303 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 362
                  ++L +  + VI+SAV A+ V +V  G +    +P+YD    S L L+LLLGAL
Sbjct: 212 --RPQFRYTLISVRA-VIISAVAANIVFRVINGQDAVITMPQYDAPELSTLGLFLLLGAL 271

Query: 363 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 422
            G+  + F+   +       K H++      L   + G   GL+ L  PE+   G   + 
Sbjct: 272 FGVFGVLFNYLITLAQDLFVKFHRNDRKRYLLTGSMIGGCFGLLLLYVPELTGGGISLIP 331

Query: 423 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 482
            +           A +L  L V +I  T LC  SG  GG +AP L +G   G A+G    
Sbjct: 332 TITNG-----GYGAGILLLLFVGRIFTTLLCFGSGAPGGIFAPMLALGTLFGYAFGLIAK 391

Query: 483 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL- 542
           +   E N            P  + + GM A  A   + P+T +LL+ E+T +Y ++LPL 
Sbjct: 392 MWFPELN----------IEPGMFAIAGMGALFAATVRAPITGILLVIEMTNNYHLILPLI 440

Query: 543 ---LGAVGVSSWLTSGQ 553
              LGAV + + L  GQ
Sbjct: 452 ITSLGAV-IFAQLLGGQ 440

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: XP_023532047.1 (chloride channel protein CLC-e [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1441 bits (3731), Expect = 0.0
Identity = 757/766 (98.83%), Postives = 757/766 (98.83%), Query Frame = 0

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS 60
           MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS
Sbjct: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS 60

Query: 61  FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEEGIPSGI 120
           FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEEGIPSGI
Sbjct: 61  FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEEGIPSGI 120

Query: 121 GSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILV 180
           GSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILV
Sbjct: 121 GSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVILV 180

Query: 181 PACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 240
           PACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG
Sbjct: 181 PACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLG 240

Query: 241 PEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWP 300
           PEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWP
Sbjct: 241 PEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWP 300

Query: 301 SPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALC 360
           SPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALC
Sbjct: 301 SPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALC 360

Query: 361 GLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDL 420
           GLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDL
Sbjct: 361 GLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDL 420

Query: 421 LLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGI 480
           LLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGI
Sbjct: 421 LLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGI 480

Query: 481 ALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLG 540
           ALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLG
Sbjct: 481 ALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLG 540

Query: 541 AVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPND 600
           AVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPND
Sbjct: 541 AVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPND 600

Query: 601 LCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALI 660
           LCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALI
Sbjct: 601 LCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALI 660

Query: 661 VDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTATPSMDILT 720
           VDEENTLIGILALQDIQKLSKN         GLVVSEVCSLDGEICRVPWTATPSMDILT
Sbjct: 661 VDEENTLIGILALQDIQKLSKNVISRTEQLKGLVVSEVCSLDGEICRVPWTATPSMDILT 720

Query: 721 AKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 757
           AKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG
Sbjct: 721 AKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 766

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: KAG6604987.1 (Chloride channel protein CLC-e, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1410 bits (3649), Expect = 0.0
Identity = 744/773 (96.25%), Postives = 752/773 (97.28%), Query Frame = 0

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS 60
           MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRP++TGFRFRS
Sbjct: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPRRTGFRFRS 60

Query: 61  FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEE------ 120
           FC+LPGSGESESPVPGSSDGRFSRGEGSPSATGIKW KEEEEEEDNTEEDEEEE      
Sbjct: 61  FCALPGSGESESPVPGSSDGRFSRGEGSPSATGIKWSKEEEEEEDNTEEDEEEEEEEEEE 120

Query: 121 -GIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEET 180
            GIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEET
Sbjct: 121 EGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEET 180

Query: 181 WKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTL 240
           WKRVILVPACGGFLVSFLNLLREAT+ND STKLGVPVSISNQFRAALQPFLKAVAASVTL
Sbjct: 181 WKRVILVPACGGFLVSFLNLLREATNNDRSTKLGVPVSISNQFRAALQPFLKAVAASVTL 240

Query: 241 GTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFA 300
           GTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFA
Sbjct: 241 GTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFA 300

Query: 301 VESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLY 360
           VESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLY
Sbjct: 301 VESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLY 360

Query: 361 LLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYW 420
           LLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYW
Sbjct: 361 LLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYW 420

Query: 421 GFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMA 480
           GFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMA
Sbjct: 421 GFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMA 480

Query: 481 YGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYR 540
           YGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYR
Sbjct: 481 YGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYR 540

Query: 541 IVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDG 600
           IVLPLLGAVGVSSWLTSGQKRKRSSQKTKKL LGK+LSTQQ+TTYDSNANDQSSNYADDG
Sbjct: 541 IVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLSLGKILSTQQTTTYDSNANDQSSNYADDG 600

Query: 601 KETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAE 660
           +ETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAE
Sbjct: 601 QETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAE 660

Query: 661 KQSYALIVDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTAT 720
           KQS ALIVDEENTLIGILAL+DIQKLSKN         GLVVSEVCSLDGEICRVPWTAT
Sbjct: 661 KQSCALIVDEENTLIGILALEDIQKLSKNVISRTEQLKGLVVSEVCSLDGEICRVPWTAT 720

Query: 721 PSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 757
           PSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESL 
Sbjct: 721 PSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLS 773

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: XP_022946999.1 (chloride channel protein CLC-e [Cucurbita moschata])

HSP 1 Score: 1400 bits (3623), Expect = 0.0
Identity = 740/767 (96.48%), Postives = 746/767 (97.26%), Query Frame = 0

Query: 6   MGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRSFCSLP 65
           MGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPK+TGFRFRSFC+LP
Sbjct: 1   MGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKRTGFRFRSFCALP 60

Query: 66  GSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEE------GIPSG 125
           GSGESESPVPGSSDGRFSRGEGSPSATGIKW KEEEEEEDNTEEDEEEE      GIPSG
Sbjct: 61  GSGESESPVPGSSDGRFSRGEGSPSATGIKWSKEEEEEEDNTEEDEEEEEEEEEEGIPSG 120

Query: 126 IGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVIL 185
           IGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVIL
Sbjct: 121 IGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVIL 180

Query: 186 VPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSL 245
           VPACGGFLVSFLNLLREATDNDPSTKL VPVSISNQFRAALQPFLKAVAASVTLGTGNSL
Sbjct: 181 VPACGGFLVSFLNLLREATDNDPSTKLRVPVSISNQFRAALQPFLKAVAASVTLGTGNSL 240

Query: 246 GPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 305
           GPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW
Sbjct: 241 GPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 300

Query: 306 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 365
           PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL
Sbjct: 301 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 360

Query: 366 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 425
           CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD
Sbjct: 361 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 420

Query: 426 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 485
           LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG
Sbjct: 421 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 480

Query: 486 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLL 545
           IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLL
Sbjct: 481 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLL 540

Query: 546 GAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPN 605
           GAVGVSSWLTSGQKRKRSSQKTKKLPLGK+LSTQQ+TTYDSNANDQSSNYADDG+ETYPN
Sbjct: 541 GAVGVSSWLTSGQKRKRSSQKTKKLPLGKILSTQQTTTYDSNANDQSSNYADDGQETYPN 600

Query: 606 DLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYAL 665
           DLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFL EAVDLMLAEKQS AL
Sbjct: 601 DLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLKEAVDLMLAEKQSCAL 660

Query: 666 IVDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTATPSMDIL 725
           IVDEENTLIGILAL+DIQKLSKN         GLVVSEV SLDGEICRVPWTATPSMDIL
Sbjct: 661 IVDEENTLIGILALEDIQKLSKNVISRTEKLKGLVVSEVFSLDGEICRVPWTATPSMDIL 720

Query: 726 TAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 757
           TAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESL 
Sbjct: 721 TAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLS 767

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: KAG7035022.1 (Chloride channel protein CLC-e, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1395 bits (3611), Expect = 0.0
Identity = 735/759 (96.84%), Postives = 742/759 (97.76%), Query Frame = 0

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS 60
           MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPK+TGFRFRS
Sbjct: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKRTGFRFRS 60

Query: 61  FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEE--GIPS 120
           FC+LPGSGESESPVPGSSDGRFSRGEGSPSATGIKW KEEEEEEDNTEEDEEEE  GIPS
Sbjct: 61  FCALPGSGESESPVPGSSDGRFSRGEGSPSATGIKWSKEEEEEEDNTEEDEEEEEEGIPS 120

Query: 121 GIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVI 180
           GIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVI
Sbjct: 121 GIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVI 180

Query: 181 LVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNS 240
           LVPACGGFLVSFLNLLREAT+NDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNS
Sbjct: 181 LVPACGGFLVSFLNLLREATNNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNS 240

Query: 241 LGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVL 300
           LGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVL
Sbjct: 241 LGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVL 300

Query: 301 WPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGA 360
           WPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGA
Sbjct: 301 WPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGA 360

Query: 361 LCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENV 420
           LCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENV
Sbjct: 361 LCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENV 420

Query: 421 DLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFI 480
           DLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFI
Sbjct: 421 DLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFI 480

Query: 481 GIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL 540
           GIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL
Sbjct: 481 GIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPL 540

Query: 541 LGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYP 600
           LGAVGVSSWLTS QKRKRSSQKTKKL LGK+LSTQQ+TTYD NANDQSSNYADDG+ETYP
Sbjct: 541 LGAVGVSSWLTSDQKRKRSSQKTKKLSLGKILSTQQTTTYDGNANDQSSNYADDGQETYP 600

Query: 601 NDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYA 660
           NDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFL EAVDLMLAEKQS A
Sbjct: 601 NDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLKEAVDLMLAEKQSCA 660

Query: 661 LIVDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTATPSMDI 720
           LIVDEENTLIGILAL+DIQKLSKN         GLVVSEVCSLDGEICRVPWTATPSMDI
Sbjct: 661 LIVDEENTLIGILALEDIQKLSKNVISRTEQLKGLVVSEVCSLDGEICRVPWTATPSMDI 720

Query: 721 LTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCR 748
           LTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCR
Sbjct: 721 LTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCR 759

BLAST of Cp4.1LG05g13220 vs. NCBI nr
Match: XP_022970939.1 (chloride channel protein CLC-e [Cucurbita maxima])

HSP 1 Score: 1392 bits (3602), Expect = 0.0
Identity = 735/771 (95.33%), Postives = 746/771 (96.76%), Query Frame = 0

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS 60
           MTVY MGA DSIG+RL NNAHHYPLSSSISAPNDCCSSYGRFL LRFSLRPK+TGFRFRS
Sbjct: 1   MTVYIMGAFDSIGVRLYNNAHHYPLSSSISAPNDCCSSYGRFLSLRFSLRPKRTGFRFRS 60

Query: 61  FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEE-----G 120
           FC+LPGSGESESPVPGSSDGRFSRGEGSPSATGIKW KEEEEE DNTEEDEEEE     G
Sbjct: 61  FCALPGSGESESPVPGSSDGRFSRGEGSPSATGIKWSKEEEEE-DNTEEDEEEEEEEEEG 120

Query: 121 IPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWK 180
           IPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWK
Sbjct: 121 IPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWK 180

Query: 181 RVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGT 240
           RVILVPACGGFLVSFLNLLREAT++DPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGT
Sbjct: 181 RVILVPACGGFLVSFLNLLREATNSDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGT 240

Query: 241 GNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVE 300
           GNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVE
Sbjct: 241 GNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVE 300

Query: 301 SVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLL 360
           SVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLL
Sbjct: 301 SVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLL 360

Query: 361 LGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGF 420
           LGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIA+AYPEILYWGF
Sbjct: 361 LGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAMAYPEILYWGF 420

Query: 421 ENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYG 480
           ENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYG
Sbjct: 421 ENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYG 480

Query: 481 KFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIV 540
           KFIGIALSEPNT IDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIV
Sbjct: 481 KFIGIALSEPNTVIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIV 540

Query: 541 LPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKE 600
           LPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGK+LSTQQSTTYDSNANDQSSNY DDGKE
Sbjct: 541 LPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKILSTQQSTTYDSNANDQSSNYTDDGKE 600

Query: 601 TYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQ 660
           TYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQ
Sbjct: 601 TYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQ 660

Query: 661 SYALIVDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTATPS 720
           S ALIVDEENTLIGILAL+DIQKLSKN         GLVVSE+CSL+GEIC+VPWTATPS
Sbjct: 661 SCALIVDEENTLIGILALEDIQKLSKNVISRTEQLKGLVVSEICSLEGEICQVPWTATPS 720

Query: 721 MDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 757
           MDILTAK VMKNLGVSQVPVVKDQMGYLVGVLDLECIDLT RILATRESLG
Sbjct: 721 MDILTAKMVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTRRILATRESLG 770

BLAST of Cp4.1LG05g13220 vs. ExPASy TrEMBL
Match: A0A6J1G5I3 (Chloride channel protein OS=Cucurbita moschata OX=3662 GN=LOC111451008 PE=3 SV=1)

HSP 1 Score: 1400 bits (3623), Expect = 0.0
Identity = 740/767 (96.48%), Postives = 746/767 (97.26%), Query Frame = 0

Query: 6   MGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRSFCSLP 65
           MGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPK+TGFRFRSFC+LP
Sbjct: 1   MGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKRTGFRFRSFCALP 60

Query: 66  GSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEE------GIPSG 125
           GSGESESPVPGSSDGRFSRGEGSPSATGIKW KEEEEEEDNTEEDEEEE      GIPSG
Sbjct: 61  GSGESESPVPGSSDGRFSRGEGSPSATGIKWSKEEEEEEDNTEEDEEEEEEEEEEGIPSG 120

Query: 126 IGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVIL 185
           IGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVIL
Sbjct: 121 IGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWKRVIL 180

Query: 186 VPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSL 245
           VPACGGFLVSFLNLLREATDNDPSTKL VPVSISNQFRAALQPFLKAVAASVTLGTGNSL
Sbjct: 181 VPACGGFLVSFLNLLREATDNDPSTKLRVPVSISNQFRAALQPFLKAVAASVTLGTGNSL 240

Query: 246 GPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 305
           GPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW
Sbjct: 241 GPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLW 300

Query: 306 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 365
           PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL
Sbjct: 301 PSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGAL 360

Query: 366 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 425
           CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD
Sbjct: 361 CGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVD 420

Query: 426 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 485
           LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG
Sbjct: 421 LLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG 480

Query: 486 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLL 545
           IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLL
Sbjct: 481 IALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLL 540

Query: 546 GAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPN 605
           GAVGVSSWLTSGQKRKRSSQKTKKLPLGK+LSTQQ+TTYDSNANDQSSNYADDG+ETYPN
Sbjct: 541 GAVGVSSWLTSGQKRKRSSQKTKKLPLGKILSTQQTTTYDSNANDQSSNYADDGQETYPN 600

Query: 606 DLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYAL 665
           DLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFL EAVDLMLAEKQS AL
Sbjct: 601 DLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLKEAVDLMLAEKQSCAL 660

Query: 666 IVDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTATPSMDIL 725
           IVDEENTLIGILAL+DIQKLSKN         GLVVSEV SLDGEICRVPWTATPSMDIL
Sbjct: 661 IVDEENTLIGILALEDIQKLSKNVISRTEKLKGLVVSEVFSLDGEICRVPWTATPSMDIL 720

Query: 726 TAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 757
           TAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESL 
Sbjct: 721 TAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLS 767

BLAST of Cp4.1LG05g13220 vs. ExPASy TrEMBL
Match: A0A6J1I747 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111469757 PE=3 SV=1)

HSP 1 Score: 1392 bits (3602), Expect = 0.0
Identity = 735/771 (95.33%), Postives = 746/771 (96.76%), Query Frame = 0

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSSSISAPNDCCSSYGRFLGLRFSLRPKQTGFRFRS 60
           MTVY MGA DSIG+RL NNAHHYPLSSSISAPNDCCSSYGRFL LRFSLRPK+TGFRFRS
Sbjct: 1   MTVYIMGAFDSIGVRLYNNAHHYPLSSSISAPNDCCSSYGRFLSLRFSLRPKRTGFRFRS 60

Query: 61  FCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEEDNTEEDEEEE-----G 120
           FC+LPGSGESESPVPGSSDGRFSRGEGSPSATGIKW KEEEEE DNTEEDEEEE     G
Sbjct: 61  FCALPGSGESESPVPGSSDGRFSRGEGSPSATGIKWSKEEEEE-DNTEEDEEEEEEEEEG 120

Query: 121 IPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWK 180
           IPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWK
Sbjct: 121 IPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLREMPVEETWK 180

Query: 181 RVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGT 240
           RVILVPACGGFLVSFLNLLREAT++DPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGT
Sbjct: 181 RVILVPACGGFLVSFLNLLREATNSDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGT 240

Query: 241 GNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVE 300
           GNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVE
Sbjct: 241 GNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVE 300

Query: 301 SVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLL 360
           SVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLL
Sbjct: 301 SVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLL 360

Query: 361 LGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGF 420
           LGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIA+AYPEILYWGF
Sbjct: 361 LGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAMAYPEILYWGF 420

Query: 421 ENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYG 480
           ENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYG
Sbjct: 421 ENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYG 480

Query: 481 KFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIV 540
           KFIGIALSEPNT IDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIV
Sbjct: 481 KFIGIALSEPNTVIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIV 540

Query: 541 LPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKE 600
           LPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGK+LSTQQSTTYDSNANDQSSNY DDGKE
Sbjct: 541 LPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKILSTQQSTTYDSNANDQSSNYTDDGKE 600

Query: 601 TYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQ 660
           TYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQ
Sbjct: 601 TYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQ 660

Query: 661 SYALIVDEENTLIGILALQDIQKLSKN---------GLVVSEVCSLDGEICRVPWTATPS 720
           S ALIVDEENTLIGILAL+DIQKLSKN         GLVVSE+CSL+GEIC+VPWTATPS
Sbjct: 661 SCALIVDEENTLIGILALEDIQKLSKNVISRTEQLKGLVVSEICSLEGEICQVPWTATPS 720

Query: 721 MDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTCRILATRESLG 757
           MDILTAK VMKNLGVSQVPVVKDQMGYLVGVLDLECIDLT RILATRESLG
Sbjct: 721 MDILTAKMVMKNLGVSQVPVVKDQMGYLVGVLDLECIDLTRRILATRESLG 770

BLAST of Cp4.1LG05g13220 vs. ExPASy TrEMBL
Match: A0A6J1CIR4 (chloride channel protein CLC-e OS=Momordica charantia OX=3673 GN=LOC111011346 PE=3 SV=1)

HSP 1 Score: 1163 bits (3009), Expect = 0.0
Identity = 637/800 (79.62%), Postives = 683/800 (85.38%), Query Frame = 0

Query: 6   MGAIDSIGIRLNNN------------------AHHYPLSSSISAPNDCC----------S 65
           M A DS+GIRL+N                   A   P+S S +  N             S
Sbjct: 1   MAAFDSLGIRLHNYPPLSPPSSASLCPKFSALAFSSPISPSNNPTNPISNGAEIFGVGGS 60

Query: 66  SYGRFLGLRFSLRPKQTGFRF-RSFCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKW 125
           S G  LGLR+SLR K+TG RF RS C LPGSGESESPV  SSD R SRG+G PS T I+ 
Sbjct: 61  SCGSVLGLRYSLRCKRTGLRFFRSICVLPGSGESESPV--SSDARLSRGDGVPSVTRIER 120

Query: 126 RKEEEE--EEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFW 185
            +E EE  EE+  EE+EEEEGIPSGIGSSTIISSCFVG+LTGIGVVLFNNAVHE+R FFW
Sbjct: 121 SEEVEEGQEEEEEEEEEEEEGIPSGIGSSTIISSCFVGLLTGIGVVLFNNAVHEIRGFFW 180

Query: 186 DGIPNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD--------NDPSTKL 245
           DGIPNRGASWLREMPVE+ WKRVILVPACGGFLVSFLNLLR+ATD           S K 
Sbjct: 181 DGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRPEKGGSSAKF 240

Query: 246 GVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRT 305
           GVP+S SN+F+AALQPFLKA+AAS+TLGTGNSLGPEGPSVDIGTSVGKG+S+VFD+NS+T
Sbjct: 241 GVPLSTSNKFKAALQPFLKAIAASITLGTGNSLGPEGPSVDIGTSVGKGISSVFDKNSQT 300

Query: 306 KLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAV 365
           KLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADST SLTNTTSMVILSAVIAS V
Sbjct: 301 KLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVV 360

Query: 366 SQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFG 425
           SQVGLGVEPAFKVPEYDFRSPSELPLYLLLG LCGLVSLSFSKCTSYMLAT+DK HKDFG
Sbjct: 361 SQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYMLATIDKFHKDFG 420

Query: 426 VSRALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILA 485
             RALFP+LGGF+TGLIALAYPEILYWGFENVDLLLESRPFVK LSA+LLAQLVV+KILA
Sbjct: 421 APRALFPVLGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKGLSADLLAQLVVIKILA 480

Query: 486 TSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVG 545
           TSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIA+S+ N  ID SIFEVASPQAYGLVG
Sbjct: 481 TSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIAVSDANPIIDISIFEVASPQAYGLVG 540

Query: 546 MAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPL 605
           MAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKR+SQ+TKKLPL
Sbjct: 541 MAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRTSQQTKKLPL 600

Query: 606 GKVLSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICV 665
            + L T+QST YDSNANDQSSNYADDGK T  NDLCEIESSLCAYDSDS+IVELERKI V
Sbjct: 601 ERSLGTRQSTAYDSNANDQSSNYADDGKATNQNDLCEIESSLCAYDSDSDIVELERKISV 660

Query: 666 SEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG--- 725
           SEAMTTRYVTV M TFL+EAV+LMLAEKQS ALIVDEENTLIGILAL+DIQKLSKN    
Sbjct: 661 SEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIGILALEDIQKLSKNAKSR 720

Query: 726 ------LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGV 757
                   VSE+CSLDGEICRVPWTATPSMD+LTA+ +MK LGV+QVPVVKDQMGYLVGV
Sbjct: 721 SERLKEAAVSEICSLDGEICRVPWTATPSMDVLTARMIMKKLGVTQVPVVKDQMGYLVGV 780

BLAST of Cp4.1LG05g13220 vs. ExPASy TrEMBL
Match: A0A1S3BJ09 (Chloride channel protein OS=Cucumis melo OX=3656 GN=LOC103490437 PE=3 SV=1)

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 621/806 (77.05%), Postives = 681/806 (84.49%), Query Frame = 0

Query: 1   MTVYTMGAIDSIGIRLNNNAHHYPLSS-----------------SISAPNDCCS------ 60
           M +  +GA DS+G++LNN  ++  LSS                 SISA +D  +      
Sbjct: 1   MIISIIGAFDSMGLKLNNAPNYLGLSSLPSASFSSNFSTLTFSSSISALHDLSNPISNGL 60

Query: 61  --------SYGRFLGLRFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGSP 120
                   SY   LGL FSLRPK+T   FRS  +LPGS ESESP+  SS+  FS      
Sbjct: 61  KNCAVGNRSYDSLLGLHFSLRPKRTASYFRSISALPGSEESESPISVSSNAAFS------ 120

Query: 121 SATGIKWRKEEEEEEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHEL 180
               IK  +EEEE++D+  E+EEEEGIP GIGSSTIISSCFVG+LTGIGVVLFNNAVHE+
Sbjct: 121 ----IKQSEEEEEDDDDDAEEEEEEGIPYGIGSSTIISSCFVGLLTGIGVVLFNNAVHEI 180

Query: 181 RDFFWDGIPNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD---------N 240
           R+F WDGIPNRGASWLREMP+E+ WKRVILVPA GG LVSFLNLLR+ATD         +
Sbjct: 181 RNFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGALVSFLNLLRDATDVKVDQPQEGD 240

Query: 241 DPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVF 300
           DPSTK GVP+SISN+ + ALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF
Sbjct: 241 DPSTKFGVPISISNKLKVALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVF 300

Query: 301 DRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSA 360
           ++NSRTKLSLIAAGSAAGISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSA
Sbjct: 301 EKNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSA 360

Query: 361 VIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDK 420
           VIAS VSQVGLG EPAFKVP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK
Sbjct: 361 VIASVVSQVGLGAEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDK 420

Query: 421 VHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV 480
            HKDFG  RA+FPILGGF+TGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV
Sbjct: 421 FHKDFGAPRAMFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLV 480

Query: 481 VVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQ 540
           V+KILATSLCRA GLVGGYYAPSLFIGAATGMAYGKFIG+ALSEPN+ +DFSIFEVASPQ
Sbjct: 481 VIKILATSLCRACGLVGGYYAPSLFIGAATGMAYGKFIGLALSEPNSVLDFSIFEVASPQ 540

Query: 541 AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQK 600
           AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+
Sbjct: 541 AYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQ 600

Query: 601 TKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVEL 660
           TKKL  GK  STQQST YDSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VEL
Sbjct: 601 TKKLSSGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVEL 660

Query: 661 ERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLS 720
           ERKI VSEAMTT+Y+T+ M T L+EAV+LMLAEKQS  LIVDE N LIGIL L++IQKLS
Sbjct: 661 ERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCVLIVDEGNILIGILELENIQKLS 720

Query: 721 KNG---------LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQM 757
           KN          +VVSE+CSLDG++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQM
Sbjct: 721 KNAKSRGEQLKDVVVSEICSLDGKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQM 780

BLAST of Cp4.1LG05g13220 vs. ExPASy TrEMBL
Match: A0A0A0KAV3 (Chloride channel protein OS=Cucumis sativus OX=3659 GN=Csa_6G116660 PE=3 SV=1)

HSP 1 Score: 1101 bits (2847), Expect = 0.0
Identity = 579/676 (85.65%), Postives = 620/676 (91.72%), Query Frame = 0

Query: 99  EEEEEEDNTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIP 158
           EEEEEE+  EE+EEEEGIP G GSSTIISSCFVG+LTGIGVVLFNNAVHE+RDF WDGIP
Sbjct: 277 EEEEEEEEEEEEEEEEGIPFGTGSSTIISSCFVGLLTGIGVVLFNNAVHEIRDFSWDGIP 336

Query: 159 NRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREATD--------NDPSTKLGVPV 218
           NRGASWLREMP+E+ WKRVILVPA GGFLVSFLNLLR+ATD        +DPSTK GVP+
Sbjct: 337 NRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQPQGDDPSTKFGVPI 396

Query: 219 SISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSL 278
           SISN+ +AALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKG+STVF+ NSRTKLSL
Sbjct: 397 SISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEMNSRTKLSL 456

Query: 279 IAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVG 338
           IAAGSAAGISSGFNAAVAGCFFA+ESVLWPSPADSTFSLTNTTSMVILSAVIAS VSQVG
Sbjct: 457 IAAGSAAGISSGFNAAVAGCFFAIESVLWPSPADSTFSLTNTTSMVILSAVIASVVSQVG 516

Query: 339 LGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRA 398
           LGVEPAFKVP YDFRSPSELPLYLLLG LCGLVSLSFSKCTSY+LATVDK HK+FG  RA
Sbjct: 517 LGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSKCTSYLLATVDKFHKEFGAPRA 576

Query: 399 LFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLC 458
           +FPILGGF+ GLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVV+KILATSLC
Sbjct: 577 MFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVIKILATSLC 636

Query: 459 RASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAAT 518
           RASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPN+ +DFSIFEVASPQAYGLVGMAAT
Sbjct: 637 RASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMAAT 696

Query: 519 LAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVL 578
           LAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+TSGQKRKRSSQ+TKKL  GK  
Sbjct: 697 LAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQQTKKLSPGKSP 756

Query: 579 STQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAM 638
           STQQST YDSNAN+QSSNYA+DG+  YPNDLCEIESSLCAY+SDSE VELERKI VSEAM
Sbjct: 757 STQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLCAYESDSETVELERKISVSEAM 816

Query: 639 TTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSKNG------- 698
           TT+Y+T+ M T L+EAV+LMLAEKQS ALIVDE N LIGIL L+DIQKLSKN        
Sbjct: 817 TTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIGILELEDIQKLSKNAKSRNEQL 876

Query: 699 --LVVSEVCSLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMGYLVGVLDLE 757
              VVSE+CSLD ++CRVPWTATPSMDILTAK +MKNLGV+QVPVV+DQMGY+VGVLD E
Sbjct: 877 KDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLGVTQVPVVRDQMGYVVGVLDWE 936

BLAST of Cp4.1LG05g13220 vs. TAIR 10
Match: AT4G35440.1 (chloride channel E )

HSP 1 Score: 734.2 bits (1894), Expect = 1.1e-211
Identity = 409/661 (61.88%), Postives = 490/661 (74.13%), Query Frame = 0

Query: 108 EEDEEEEGIPSGIGSS-TIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLR 167
           E D++E G          I S+C VGVLTG+ VVLFNN VH LRDF WDGIP+RGASWLR
Sbjct: 59  ETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLR 118

Query: 168 EMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKA 227
           E P+   W RVILVP  GG +VS LN LRE+         G   S  ++ +A L+PFLK 
Sbjct: 119 EAPIGSNWLRVILVPTIGGLVVSILNQLRESA----GKSTGDSHSSLDRVKAVLRPFLKT 178

Query: 228 VAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAA 287
           VAA VTLGTGNSLGPEGPSV+IG S+ KGV+++F+++ +T  SL+AAGSAAGISSGFNAA
Sbjct: 179 VAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLFNKSPQTGFSLLAAGSAAGISSGFNAA 238

Query: 288 VAGCFFAVESVLWPSPA-DSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFR 347
           VAGCFFAVESVLWPS + DS+ SL NTTSMVILSAV AS VS++GLG EPAFKVP+YDFR
Sbjct: 239 VAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFR 298

Query: 348 SPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAL 407
           SP ELPLYLLLGALCGLVSL+ S+CTS M + VD ++KD G+ +A+FP++GG S G+IAL
Sbjct: 299 SPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIAL 358

Query: 408 AYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLF 467
            YPE+LYWGF+NVD+LLE RPFVK LSA+LL QLV VKI AT+ CRASGLVGGYYAPSLF
Sbjct: 359 VYPEVLYWGFQNVDILLEKRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLF 418

Query: 468 IGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 527
           IG A GMAYGKFIG+AL++ N   + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLL
Sbjct: 419 IGGAAGMAYGKFIGLALAQ-NPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 478

Query: 528 FELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQ 587
           FELTQDYRIVLPLLGAVG+SSW+TSGQ +++ +++TK+    K     QS T   +++D+
Sbjct: 479 FELTQDYRIVLPLLGAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDE 538

Query: 588 SSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIE 647
           SS           N+LCE+ESSLC  DS ++  EL + I VSEAM TR+ TV M T L E
Sbjct: 539 SST----------NNLCEVESSLCLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEE 598

Query: 648 AVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSK---------NGLVVSEVCSLDGEI 707
           A+  ML EKQS ALIVD +N  +GIL L DIQ+ SK           + V+++CS  G  
Sbjct: 599 ALTRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGK 658

Query: 708 CRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMG----YLVGVLDLECIDLTCRILAT 754
           C+VPWT TP MD+L A+T+M    +S V VV   +     + VGVLD ECI LT R LAT
Sbjct: 659 CKVPWTVTPDMDLLAAQTIMNKHELSHVAVVSGSIDAPRIHPVGVLDRECITLTRRALAT 701

BLAST of Cp4.1LG05g13220 vs. TAIR 10
Match: AT4G35440.2 (chloride channel E )

HSP 1 Score: 727.6 bits (1877), Expect = 1.0e-209
Identity = 405/656 (61.74%), Postives = 486/656 (74.09%), Query Frame = 0

Query: 108 EEDEEEEGIPSGIGSS-TIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWLR 167
           E D++E G          I S+C VGVLTG+ VVLFNN VH LRDF WDGIP+RGASWLR
Sbjct: 59  ETDQDEVGFDQQPSQELAIASACLVGVLTGVSVVLFNNCVHLLRDFSWDGIPDRGASWLR 118

Query: 168 EMPVEETWKRVILVPACGGFLVSFLNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKA 227
           E P+   W RVILVP  GG +VS LN LRE+         G   S  ++ +A L+PFLK 
Sbjct: 119 EAPIGSNWLRVILVPTIGGLVVSILNQLRESA----GKSTGDSHSSLDRVKAVLRPFLKT 178

Query: 228 VAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAA 287
           VAA VTLGTGNSLGPEGPSV+IG S+ KGV+++F+++ +T  SL+AAGSAAGISSGFNAA
Sbjct: 179 VAACVTLGTGNSLGPEGPSVEIGASIAKGVNSLFNKSPQTGFSLLAAGSAAGISSGFNAA 238

Query: 288 VAGCFFAVESVLWPSPA-DSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFR 347
           VAGCFFAVESVLWPS + DS+ SL NTTSMVILSAV AS VS++GLG EPAFKVP+YDFR
Sbjct: 239 VAGCFFAVESVLWPSSSTDSSTSLPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFR 298

Query: 348 SPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAL 407
           SP ELPLYLLLGALCGLVSL+ S+CTS M + VD ++KD G+ +A+FP++GG S G+IAL
Sbjct: 299 SPGELPLYLLLGALCGLVSLALSRCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIAL 358

Query: 408 AYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLF 467
            YPE+LYWGF+NVD+LLE RPFVK LSA+LL QLV VKI AT+ CRASGLVGGYYAPSLF
Sbjct: 359 VYPEVLYWGFQNVDILLEKRPFVKGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLF 418

Query: 468 IGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 527
           IG A GMAYGKFIG+AL++ N   + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLL
Sbjct: 419 IGGAAGMAYGKFIGLALAQ-NPDFNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLL 478

Query: 528 FELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQ 587
           FELTQDYRIVLPLLGAVG+SSW+TSGQ +++ +++TK+    K     QS T   +++D+
Sbjct: 479 FELTQDYRIVLPLLGAVGMSSWITSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDE 538

Query: 588 SSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIE 647
           SS           N+LCE+ESSLC  DS ++  EL + I VSEAM TR+ TV M T L E
Sbjct: 539 SST----------NNLCEVESSLCLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEE 598

Query: 648 AVDLMLAEKQSYALIVDEENTLIGILALQDIQKLSK---------NGLVVSEVCSLDGEI 707
           A+  ML EKQS ALIVD +N  +GIL L DIQ+ SK           + V+++CS  G  
Sbjct: 599 ALTRMLIEKQSCALIVDPDNIFLGILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGK 658

Query: 708 CRVPWTATPSMDILTAKTVMKNLGVSQVPVVKDQMG----YLVGVLDLECIDLTCR 749
           C+VPWT TP MD+L A+T+M    +S V VV   +     + VGVLD ECI LT R
Sbjct: 659 CKVPWTVTPDMDLLAAQTIMNKHELSHVAVVSGSIDAPRIHPVGVLDRECITLTRR 696

BLAST of Cp4.1LG05g13220 vs. TAIR 10
Match: AT1G55620.2 (chloride channel F )

HSP 1 Score: 409.8 bits (1052), Expect = 4.6e-114
Identity = 283/700 (40.43%), Postives = 397/700 (56.71%), Query Frame = 0

Query: 46  RFSLRPKQTGFRFRSFCSLPGSGESESPVPGSSDGRFSRGEGSPSATGIKWRKEEEEEED 105
           RFSL  ++  F+     ++    E  +P   SS   FS   G     GI      ++  D
Sbjct: 59  RFSLSGRRLSFKRME--NIRVDRERHNP---SSSSAFS-AAGEEDGGGISNLHSVDDRND 118

Query: 106 NTEEDEEEEGIPSGIGSSTIISSCFVGVLTGIGVVLFNNAVHELRDFFWDGIPNRGASWL 165
               DEE  G  +    + ++  C +GV  GI V  FN  VH + ++ W G PN GA+WL
Sbjct: 119 EYGFDEEVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWL 178

Query: 166 REMPVEETWKRVILVPACGGFLVSFLNLLREATD----NDPSTKLGVPVSISNQFRAALQ 225
           R   + +TW R++L+P  GG +V  ++ L E  D    ++ S + G+       F A + 
Sbjct: 179 RLQRLADTWHRILLIPVTGGVIVGMMHGLLEILDQIRQSNSSQRQGL------DFLAGIY 238

Query: 226 PFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVSTVFDRNSRTKLSLIAAGSAAGISS 285
           P +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + + N   +++L AAG+A+GI+S
Sbjct: 239 PVIKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGAASGIAS 298

Query: 286 GFNAAVAGCFFAVESVLWPSPADSTFSLTNTTSMVILSAVIASAVSQVGLGVEPAFKVPE 345
           GFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS   LG + AF VP 
Sbjct: 299 GFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVSNALLGTQSAFTVPS 358

Query: 346 YDFRSPSELPLYLLLGALCGLVSLSFSKCTSYMLATVDKVHKDFGVSRALFPILGGFSTG 405
           YD +S +ELPLYL+LG LCG VS+ FS+  ++   + D +   FG+   + P LGG   G
Sbjct: 359 YDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPALGGLGAG 418

Query: 406 LIALAYPEILYWGFENVDLLLESRPFVKPLSAELLAQLVVVKILATSLCRASGLVGGYYA 465
           +IAL YP ILYWGF NV+ +L +          LLAQL   K++AT+LC+ SGLVGG YA
Sbjct: 419 IIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGLVGGLYA 478

Query: 466 PSLFIGAATGMAYGKFIGIALSEPNTAIDFSIFEVASPQAYGLVGMAATLAGVCQVPLTA 525
           PSL IGAA G  +G   G A    N AI  +   VA PQAY LVGMAATLA +C VPLT+
Sbjct: 479 PSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGMAATLASMCSVPLTS 538

Query: 526 VLLLFELTQDYRIVLPLLGAVGVSSWLTSGQKRKRSSQKTKKLPLGKVLSTQQSTTYDSN 585
           VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S  ++    G+  S+   +   + 
Sbjct: 539 VLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSPSERKTE 598

Query: 586 ANDQSSNYADDGKETYPNDLCEIESSLCAYDSDSEIVELERKICVSEAMTTRYVTVFMDT 645
              + ++ AD        +L  IE+       D E +  + K  V   M+  YV V   T
Sbjct: 599 GVWRHTDNADS------LELTVIENPDHNSFLDEETILEDLK--VMRVMSKNYVKVSSGT 658

Query: 646 FLIEAVDLMLAEKQSYALIVDEENTLIGILALQDIQK---------LSKNGLVVSEVC-- 705
            L EA +++    Q+  ++VD+++ L GIL   DI++         L +N   VS VC  
Sbjct: 659 TLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTILDENTCPVSSVCTK 718

Query: 706 --SLDGEICRVPWTATPSMDILTAKTVMKNLGVSQVPVVK 729
             S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 719 KISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 731

BLAST of Cp4.1LG05g13220 vs. TAIR 10
Match: AT1G55620.1 (chloride channel F )

HSP 1 Score: 352.4 bits (903), Expect = 8.8e-97
Identity = 236/551 (42.83%), Postives = 327/551 (59.35%), Query Frame = 0

Query: 191 LNLLREATDNDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGT 250
           L +L +   ++ S + G+       F A + P +KA+ A+VTLGTG SLGPEGPSVDIG 
Sbjct: 6   LEILDQIRQSNSSQRQGL------DFLAGIYPVIKAIQAAVTLGTGCSLGPEGPSVDIGK 65

Query: 251 SVGKGVSTVFDRNSRTKLSLIAAGSAAGISSGFNAAVAGCFFAVESVLWPSPADSTFSLT 310
           S   G + + + N   +++L AAG+A+GI+SGFNAAVAGCFFA+E+VL P  A++  S  
Sbjct: 66  SCANGFALMMENNRERRIALTAAGAASGIASGFNAAVAGCFFAIETVLRPLRAEN--SPP 125

Query: 311 NTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSKC 370
            TT+M+IL++VI+S VS   LG + AF VP YD +S +ELPLYL+LG LCG VS+ FS+ 
Sbjct: 126 FTTAMIILASVISSTVSNALLGTQSAFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRL 185

Query: 371 TSYMLATVDKVHKDFGVSRALFPILGGFSTGLIALAYPEILYWGFENVDLLLESRPFVKP 430
            ++   + D +   FG+   + P LGG   G+IAL YP ILYWGF NV+ +L +      
Sbjct: 186 VTWFTKSFDFIKDKFGLPAIVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGKSASA 245

Query: 431 LSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTAID 490
               LLAQL   K++AT+LC+ SGLVGG YAPSL IGAA G  +G   G A    N AI 
Sbjct: 246 PGIWLLAQLAAAKVVATALCKGSGLVGGLYAPSLMIGAAVGAVFG---GSAAEIINRAIP 305

Query: 491 FSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLTS 550
            +   VA PQAY LVGMAATLA +C VPLT+VLLLFELT+DYRI+LPL+GAVG++ W+ S
Sbjct: 306 GNA-AVAQPQAYALVGMAATLASMCSVPLTSVLLLFELTKDYRILLPLMGAVGLAIWVPS 365

Query: 551 GQKRKRSSQKTKKLPLGKVLSTQQSTTYDSNANDQSSNYADDGKETYPNDLCEIESSLCA 610
              + + S  ++    G+  S+   +   +    + ++ AD        +L  IE+    
Sbjct: 366 VANQGKESDSSEGRSTGRGYSSLSPSERKTEGVWRHTDNADS------LELTVIENPDHN 425

Query: 611 YDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSYALIVDEENTLIGI 670
              D E +  + K  V   M+  YV V   T L EA +++    Q+  ++VD+++ L GI
Sbjct: 426 SFLDEETILEDLK--VMRVMSKNYVKVSSGTTLREARNILKESHQNCIMVVDDDDFLAGI 485

Query: 671 LALQDIQK---------LSKNGLVVSEVC----SLDGEICRVPWTATPSMDILTAKTVMK 729
           L   DI++         L +N   VS VC    S  G+  R   T  P   +  AK +M+
Sbjct: 486 LTHGDIRRYLSNNASTILDENTCPVSSVCTKKISYRGQE-RGLLTCYPDATVGVAKELME 535