Cp4.1LG05g12280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG05g12280 vs. ExPASy Swiss-Prot
Match: O80462 (Extra-large guanine nucleotide-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=XLG1 PE=1 SV=2)

HSP 1 Score: 726.5 bits (1874), Expect = 3.6e-208
Identity = 417/895 (46.59%), Postives = 549/895 (61.34%), Query Frame = 0

Query: 28  FAIEYTGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVSSLPVIQPIVGKFSRSSSL 87
           FA EY GP ++Y+IP AVPIN++ IP A+VV  S     D  S PVIQPI+   S+   +
Sbjct: 11  FAEEYDGPPLSYNIPCAVPINVEKIPVAAVV--SPVCISDNMSFPVIQPILSVESKKFLI 70

Query: 88  KANLVISSTSEIQEDGPVCLDANKKDNGKDKCDIIESSGELENFN-------KLKGRLEG 147
            +   +S TS I   G   L+            +IE + E E          +L    E 
Sbjct: 71  DS---VSPTSVIANCGSNQLELVSDSITVSPTSVIEHTEEEEEEEGGDGEDCELSSSGEL 130

Query: 148 GLESLEIKNEEDFQGYTNS---SDSESAESGLSSSSGIFAVRGEEEVENEIQPWHGRKPS 207
            L S  +K   D    +++    D ES ES LS       V G+   E       G+K  
Sbjct: 131 LLRSCSVKESLDLNESSSNPLVPDWESNESVLSMDYPSSRVTGDCVSETN---GDGKKQP 190

Query: 208 AVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIV 267
            VTFL   S      +E+ S+      +    + + KGKKGSCY C KG+RFTEKE C+V
Sbjct: 191 VVTFLGIASDDGFEEEESCSNLRRVRVVPVKKQPQTKGKKGSCYRCFKGSRFTEKEVCLV 250

Query: 268 CGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKNTLT 327
           C AKYC +CV+RAMGSMPEGRKC+TCIGF IDES+R +LGKC+++LKRL  DLEVK  + 
Sbjct: 251 CDAKYCNSCVLRAMGSMPEGRKCVTCIGFPIDESKRGSLGKCSRMLKRLLNDLEVKQIMK 310

Query: 328 REKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRG 387
            E+ CE NQ+PA  VY+N  PL  +EL+ L++C  PPK LKPG YWYDK SG WGKEG  
Sbjct: 311 TERFCEANQLPAEYVYVNGQPLYPEELVTLQTCSNPPKKLKPGDYWYDKVSGLWGKEGEK 370

Query: 388 PCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADG 447
           P QI+S HL VGG I  +ASNGNT V IN REITK ELR+L+LAGV C G P +WV+ DG
Sbjct: 371 PYQIISPHLNVGGPISPEASNGNTQVFINGREITKVELRMLQLAGVQCAGNPHFWVNEDG 430

Query: 448 SYQEEGM-NNGGKIWDKPTTKLACAVFNLPIPSCSV--HSGEE----------------- 507
           SYQEEG  N  G IW K  TKL CAV +LP+PS S    SGE+                 
Sbjct: 431 SYQEEGQKNTKGYIWGKAGTKLLCAVLSLPVPSKSTANASGEQLYSANSRSILDHLEHRT 490

Query: 508 -------------------NAKQIYK-VPFSDDERQAIKFLIQRKLYWYISILLEGRERF 567
                               AK +YK VPF +DER+ IK +IQ  +Y Y+ +LLEGRERF
Sbjct: 491 LQKILLVGNSGSGTSTIFKQAKILYKDVPFLEDERENIKVIIQTNVYGYLGMLLEGRERF 550

Query: 568 EEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETT 627
           EEE L     KQ V +   +    +    +  T+YS GP+LK F+DWLL+ + +G     
Sbjct: 551 EEEALALRNTKQCVLENIPADEGDAKSNDKTVTMYSIGPRLKAFSDWLLKTMAAGNLGVI 610

Query: 628 FPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSDNDI 687
           FPAA+R YA  VEEL +D A QATY RR+EL +LP V++YFL+RA+D+ + DY+PSD DI
Sbjct: 611 FPAASREYAPLVEELWRDAAIQATYKRRSELGLLPSVASYFLERAIDVLTPDYEPSDLDI 670

Query: 688 LYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLDMF 747
           LYAEG++S   L+ ++FSFP++  +  LDP   HDS +RYQLIRV    LGENCK +DMF
Sbjct: 671 LYAEGVTSSSGLACLDFSFPQTASEENLDPSDHHDSLLRYQLIRVPSRGLGENCKWIDMF 730

Query: 748 EDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNFLLILNKFDL 807
           ED+ +++F V++ DYD+  ED      N+M+ +K+LFE I+T       +FLLILNK+DL
Sbjct: 731 EDVGMVVFVVSMSDYDQVSEDG----TNKMLLTKKLFESIITHPIFENMDFLLILNKYDL 790

Query: 808 FEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYIAVKFKRLFY 867
            EEK+ +VPL++CEWF DFNP++       S    S++ NP+L Q AF ++AVKFKR + 
Sbjct: 791 LEEKVERVPLARCEWFQDFNPVV-------SRHRGSNNGNPTLGQLAFHFMAVKFKRFYS 850

Query: 868 SLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASADASSF 873
           SLT KKLFVS +  ++P +V+++L+ A EI+KW  ++ NI ++E S  S + SSF
Sbjct: 851 SLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSF 886

BLAST of Cp4.1LG05g12280 vs. ExPASy Swiss-Prot
Match: C6KIE6 (Extra-large guanine nucleotide-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=XLG2 PE=1 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.9e-188
Identity = 398/911 (43.69%), Postives = 546/911 (59.93%), Query Frame = 0

Query: 6   KKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVSSSQFR 65
           K+   E       +  +  EYSFA EY GP I  ++PRA+P+ +D IPTA + VS S  R
Sbjct: 17  KRDNRESDDDDETSSGYRIEYSFASEYKGPLI-ANVPRALPVEVDQIPTA-LPVSFSSLR 76

Query: 66  DDVSSLPVIQPIVGKFSR----SSSLKANLVISST--SEIQEDGPVCLDANKKDNGKDKC 125
             + S PV   ++ K ++    S   K N  + S   S +   G   +  +   +   + 
Sbjct: 77  SGI-SYPVAPLVMTKDTKRPPDSGIEKKNGFVDSAAGSSVVLIGRDVVSGSSSSSSSKRL 136

Query: 126 DIIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVR 185
           D+ E                      E+K+  DF+         S  S LS+S+     R
Sbjct: 137 DVPE----------------------EVKSPADFR--------LSPSSPLSASA-----R 196

Query: 186 GEEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGS 245
            E+ ++++     G  P AV F++P  SS     +  S  SD ESI    RAERKGK+GS
Sbjct: 197 EEDHLDDDRVSDVG--PRAVRFVEPFQSSEC---DESSYVSDGESIAATHRAERKGKRGS 256

Query: 246 CYYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKC 305
           CY C  GNRFTEKE CIVC AKYC  CV RAMG+MPEGRKC  CIG+RIDES+R +LGKC
Sbjct: 257 CYRCQLGNRFTEKEVCIVCDAKYCFNCVRRAMGAMPEGRKCQACIGYRIDESKRASLGKC 316

Query: 306 TKVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKP 365
           +++LKR  TD E++  +  E  C+ NQ+P+RL+ +ND PLS  EL  L++C  PPK LKP
Sbjct: 317 SRMLKRHLTDSELRQVMNAEITCKANQLPSRLIIVNDKPLSEDELYTLQTCPNPPKKLKP 376

Query: 366 GRYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILK 425
           G YWYDK +G+WGK G  P QI+S +  +GG I    SNG+T + IN REITK EL +LK
Sbjct: 377 GHYWYDKVAGYWGKIGEKPSQIISPNNSIGGYISEKVSNGDTEIYINGREITKPELTMLK 436

Query: 426 LAGVPCEGRPSYWVSADGSYQEEGMNNG-GKIWDKPTTKLACAVFNLPIPSCS------- 485
            AGV CEG+P +WV +DGSY+EEG  +  G IW K   K+ACAVF+LP+P  S       
Sbjct: 437 WAGVQCEGKPHFWVDSDGSYREEGQKHPIGNIWSKKRAKIACAVFSLPVPPASSAVEPYD 496

Query: 486 ------------VHSGEE---------NAKQIYKVPFSDDERQAIKFLIQRKLYWYISIL 545
                       +  G E          A+ +Y V FS ++R+ IKF+IQ  LY Y++++
Sbjct: 497 VPLYEQKMLNKLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMV 556

Query: 546 LEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVV 605
           LE  ERFE+E+  D+ +   V D  TS+  G+          S  P+LK F+DW+L+   
Sbjct: 557 LEAHERFEKEMSNDQSSGN-VGD-ETSAKPGN----------SINPRLKHFSDWVLKEKE 616

Query: 606 SGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADY 665
            G  +  FP ++R  AQ+V +L +  A QATY R    + LPR + YFL+R ++IS ++Y
Sbjct: 617 DGNLK-IFPPSSRENAQTVADLWRVPAIQATYKRLR--DTLPRNAVYFLERILEISRSEY 676

Query: 666 DPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLGEN 725
           DPSD DIL AEG+SS + LS ++FSFP + Q+  L+  YQHD+ ++YQLIR++P +LGEN
Sbjct: 677 DPSDMDILQAEGLSSMEGLSCVDFSFPSTSQEESLESDYQHDTDMKYQLIRLNPRSLGEN 736

Query: 726 CKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNFLL 785
            KLL+MFED  +++FCV+L DY E  ED  G +VN+M+A+KQLFE +VT  +L+ K FLL
Sbjct: 737 WKLLEMFEDADLVIFCVSLTDYAENIEDGEGNIVNKMLATKQLFENMVTHPSLANKRFLL 796

Query: 786 ILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYIAV 845
           +L KFDL EEKI +VPL  CEWF+DFNP+I+ N T R         NP +AQRAF YI  
Sbjct: 797 VLTKFDLLEEKIEEVPLRTCEWFEDFNPLISQNQTSRH--------NPPMAQRAFHYIGY 856

Query: 846 KFKRLFYSLTD----------KKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISIT 872
           KFKRL+ S+ +           KLFV Q + +E + V+ ALRYAREI+KW V++ ++   
Sbjct: 857 KFKRLYDSILEPVNMRGRSFKPKLFVCQVS-LESDTVDNALRYAREILKWHVEETSM-FQ 859

BLAST of Cp4.1LG05g12280 vs. ExPASy Swiss-Prot
Match: Q9C516 (Extra-large guanine nucleotide-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=XLG3 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.2e-152
Identity = 323/787 (41.04%), Postives = 453/787 (57.56%), Query Frame = 0

Query: 155 GYTNSS-----DSESAESGL-------SSSSGIFAVRGEEEVENEIQPWHGRKPSAVTF- 214
           G T+SS      SES  S L       S S+ +  V G  +  N++     R+P  V F 
Sbjct: 74  GVTSSSGGSPASSESVVSVLHNNPESSSGSASVSPVSGHRQNGNQV-----RRP-VVKFK 133

Query: 215 -LDPHSS-SSTISDEAESSQSDAES-----IHD---MPRAERKGKKGSCYYCLKGNRFTE 274
            +D H       + E E +  +AE+     +H+     +  +K KK  CY C K  ++  
Sbjct: 134 PVDDHDRIEGREAAEEEDNNVEAETERERKVHECTASTKRRKKKKKSECYRCGKA-KWEN 193

Query: 275 KEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLE 334
           KE CIVC  KYC  CV+RAMGSMPEGRKC++CIG  IDES+R+ LGK ++VL RL + LE
Sbjct: 194 KETCIVCDEKYCGNCVLRAMGSMPEGRKCVSCIGQAIDESKRSKLGKHSRVLSRLLSPLE 253

Query: 335 VKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFW 394
           VK  +  EKEC  NQ+    + +N +PL  +E+  L +C  PP+ LKPGRYWYDKESG W
Sbjct: 254 VKQIMKAEKECTANQLRPEQLIVNGYPLKPEEMADLLNCLLPPQKLKPGRYWYDKESGLW 313

Query: 395 GKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSY 454
           GKEG  P +++S++L   G++  DASNGNT V IN REITK ELRILKLA V C     +
Sbjct: 314 GKEGEKPDRVISSNLNFTGKLSPDASNGNTEVYINGREITKLELRILKLANVQCPRDTHF 373

Query: 455 WVSADGSYQEEGMNN-GGKIWDKPTTKLACAVFNLPIPSCSVH----------------- 514
           WV  DG Y+EEG NN  G IW+K +T+  CA+F+LP+P                      
Sbjct: 374 WVYDDGRYEEEGQNNIRGNIWEKASTRFMCALFSLPVPQGQPRGTVQPSSNYATVPNYIE 433

Query: 515 --------------SGE----ENAKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRE 574
                         SG     + AK +Y   FS +E Q IK ++Q  +Y Y+SILL+GRE
Sbjct: 434 HKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRE 493

Query: 575 RFEEEILMDEKNKQPV-NDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYF 634
           RFEEE L   +    V  D     A+        +++Y+  P+LK F+DWLL ++ +G  
Sbjct: 494 RFEEEALSHTRGLNAVEGDSGGEEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDL 553

Query: 635 ETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSD 694
           +  FPAATR YA  VEE+ KD A QATY R++EL  LP V+ YFL RA+++SS +Y+PS+
Sbjct: 554 DAFFPAATREYAPLVEEVWKDPAIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSE 613

Query: 695 NDILYAEGISSCKSLSDMEFSFPESRQDSLL---DPPYQHDSSIRYQLIRVHPSTLGENC 754
            DI+YAEG++    L+ MEFS  +    S     +P        +YQLIRV+   + ++C
Sbjct: 614 RDIVYAEGVTQGNGLAFMEFSLSDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSC 673

Query: 755 KLLDMFEDMSIILFCVALVDYDEFD--EDDNGVL--VNRMIASKQLFERIVTDRALSGKN 814
           K ++MFED+  ++FC++L DYD+ +   + +G +   N+MI SK+LFE +V         
Sbjct: 674 KWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTP 733

Query: 815 FLLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQY 872
           F+LILNK+D FEEK+ + PL+ C+WF DF P+ T N+              SLA +A+ Y
Sbjct: 734 FILILNKYDQFEEKLNRAPLTSCDWFSDFCPVRTNNNV------------QSLAYQAYFY 793

BLAST of Cp4.1LG05g12280 vs. ExPASy Swiss-Prot
Match: O04278 (Guanine nucleotide-binding protein alpha-1 subunit OS=Pisum sativum OX=3888 GN=GPA1 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.0e-28
Identity = 99/376 (26.33%), Postives = 180/376 (47.87%), Query Frame = 0

Query: 480 KQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEEILMDEKNKQPVNDPSTSSA 539
           K +++  F + E ++   ++   +Y  I +L +G + F +                    
Sbjct: 60  KLLFQTGFDEAELKSYLPVVHANVYQTIKLLHDGSKEFAQ-------------------- 119

Query: 540 SGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAFQ 599
             +DV F +   Y    + K   + L +  + G  +  +P  T+  AQ +E + KD A Q
Sbjct: 120 --NDVDFSK---YVISTENKDIGEKLSE--IGGRLD--YPRLTKELAQEIESIWKDAAIQ 179

Query: 600 ATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSDNDILYAEGISSCKSLSDMEFSFPES 659
            TY+R NEL+ +P  + YF++    +S A+Y P+  D+L A      ++   +E  F   
Sbjct: 180 ETYARGNELQ-VPDCTHYFMENLQRLSDANYVPTKEDVLLAR----VRTTGVVEIQFSPV 239

Query: 660 RQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLDMFEDMSIILFCVALVDYDE-FDED 719
            ++          S   Y+L  V      E  K + +FE +S ++FCVA+ +YD+   ED
Sbjct: 240 GENK--------KSGEVYRLFDV-GGQRNERRKWIHLFEGVSAVIFCVAISEYDQTLFED 299

Query: 720 DNGVLVNRMIASKQLFERIVTDRALSGKNFLLILNKFDLFEEKIIQVPLSKCEWFDDFNP 779
           +N    NRM+ +K+LFE ++  +     +F+L LNKFD+FE+KI+ VPL+ CEWF D+ P
Sbjct: 300 EN---KNRMMETKELFEWVLKQQCFEKTSFMLFLNKFDIFEKKILDVPLNVCEWFKDYQP 359

Query: 780 MITPNSTGRSSSSSSSSTNPSLAQRAFQYIAVKFKRLFYSLT-----DKKLFVSQTTGME 839
           +    STG+              + A++++  KF+  ++  T     D+   + +TT ++
Sbjct: 360 V----STGKQE-----------IEHAYEFVKKKFEESYFQSTAPDSVDRVFKIYRTTALD 374

Query: 840 PENVNAALRYAREIIK 850
            + V    +   E ++
Sbjct: 420 QKVVKKTFKLVDETLR 374

BLAST of Cp4.1LG05g12280 vs. ExPASy Swiss-Prot
Match: P93163 (Guanine nucleotide-binding protein alpha-2 subunit OS=Glycine max OX=3847 GN=GPA2 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 3.4e-28
Identity = 84/278 (30.22%), Postives = 145/278 (52.16%), Query Frame = 0

Query: 578 FPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSDNDI 637
           +P  ++  AQ +E L KD A Q TY+R +EL+ +P  + YF++    +S A+Y P+  D+
Sbjct: 130 YPYLSKELAQEIENLWKDPAIQETYARGSELQ-IPDCTDYFMENLQRLSDANYVPTKEDV 189

Query: 638 LYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLDMF 697
           LYA      ++   +E  F    ++   D          Y+L  V      E  K + +F
Sbjct: 190 LYAR----VRTTGVVEIQFSPVGENKKSDEV--------YRLFDV-GGQRNERRKWIHLF 249

Query: 698 EDMSIILFCVALVDYDE-FDEDDNGVLVNRMIASKQLFERIVTDRALSGKNFLLILNKFD 757
           E +S ++FC A+ +YD+   ED+N    NRM+ +K+LFE I+        +F+L LNKFD
Sbjct: 250 EGVSAVIFCAAISEYDQTLFEDENR---NRMMETKELFEWILKQPCFEKTSFMLFLNKFD 309

Query: 758 LFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYIAVKFKRLF 817
           +FE+KI++VPL+ CEWF D+ P+    STG+              + A++++  KF+  +
Sbjct: 310 IFEKKILKVPLNVCEWFKDYQPV----STGKQE-----------IEHAYEFVKKKFEESY 369

Query: 818 YSLT-----DKKLFVSQTTGMEPENVNAALRYAREIIK 850
           +  T     D+   + +TT ++ + V    +   E ++
Sbjct: 370 FQSTAPDRVDRVFKIYRTTALDQKVVKKTFKLVDETLR 375

BLAST of Cp4.1LG05g12280 vs. NCBI nr
Match: XP_023534204.1 (extra-large guanine nucleotide-binding protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1723 bits (4462), Expect = 0.0
Identity = 873/905 (96.46%), Postives = 873/905 (96.46%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS
Sbjct: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120
           SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD
Sbjct: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120

Query: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180
           IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG
Sbjct: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180

Query: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240
           EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC
Sbjct: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240

Query: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300
           YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT
Sbjct: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300

Query: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360
           KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG
Sbjct: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360

Query: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420
           RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL
Sbjct: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420

Query: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN-- 480
           AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN  
Sbjct: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEENVD 480

Query: 481 ------------------------------AKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540
                                         AKQIYKVPFSDDERQAIKFLIQRKLYWYIS
Sbjct: 481 KASSSPEPKMLHKLLLVGHDQSGTSTIFKQAKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540

Query: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600
           ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV
Sbjct: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600

Query: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660
           VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA
Sbjct: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660

Query: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720
           DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG
Sbjct: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720

Query: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780
           ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF
Sbjct: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780

Query: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840
           LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI
Sbjct: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840

Query: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 873
           AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD
Sbjct: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 900

BLAST of Cp4.1LG05g12280 vs. NCBI nr
Match: XP_022947948.1 (extra-large guanine nucleotide-binding protein 1-like [Cucurbita moschata])

HSP 1 Score: 1691 bits (4379), Expect = 0.0
Identity = 858/905 (94.81%), Postives = 866/905 (95.69%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS
Sbjct: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120
           SSQFRDDVS+LPVIQPIVG+FSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD
Sbjct: 61  SSQFRDDVSTLPVIQPIVGRFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120

Query: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180
           IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG
Sbjct: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180

Query: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240
            EEVENEIQPWHGRKPSAVTFLDPHSSS TISDEAESSQSDAESIHDMPRAERKGKKGSC
Sbjct: 181 GEEVENEIQPWHGRKPSAVTFLDPHSSS-TISDEAESSQSDAESIHDMPRAERKGKKGSC 240

Query: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300
           YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT
Sbjct: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300

Query: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360
           KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG
Sbjct: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360

Query: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420
           RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL
Sbjct: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420

Query: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN-- 480
           AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPS SVHSGEE   
Sbjct: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSSSVHSGEEKVD 480

Query: 481 ------------------------------AKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540
                                         AKQIYKVPFSDDERQAIKFLIQRKLYWYIS
Sbjct: 481 KASSSPEPKMLHKLLLVGHDQSGTSTIFKQAKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540

Query: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600
           ILLEGRERFEEE+LMDEKNKQPVNDPSTSSASG+DVQFERKTIYSFGPKLKVFADWLLQV
Sbjct: 541 ILLEGRERFEEEVLMDEKNKQPVNDPSTSSASGNDVQFERKTIYSFGPKLKVFADWLLQV 600

Query: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660
           VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISS 
Sbjct: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSE 660

Query: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720
           DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG
Sbjct: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720

Query: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780
           ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF
Sbjct: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780

Query: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840
           LLILNKFDLFEEKIIQVPLS+CEWFDDFNPMITPNSTGRSSSSS+  TNPSLAQRAFQY+
Sbjct: 781 LLILNKFDLFEEKIIQVPLSQCEWFDDFNPMITPNSTGRSSSSST--TNPSLAQRAFQYV 840

Query: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 873
           AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEIS+ASAD
Sbjct: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISTASAD 900

BLAST of Cp4.1LG05g12280 vs. NCBI nr
Match: KAG7035111.1 (Extra-large guanine nucleotide-binding protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1686 bits (4366), Expect = 0.0
Identity = 854/905 (94.36%), Postives = 866/905 (95.69%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS
Sbjct: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120
           SSQFRDDVS+LPVIQPIVG+FSRSSSLKANLVISSTS+IQEDGPVCLDANKKDNGKDKCD
Sbjct: 61  SSQFRDDVSTLPVIQPIVGRFSRSSSLKANLVISSTSDIQEDGPVCLDANKKDNGKDKCD 120

Query: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180
           IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG
Sbjct: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180

Query: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240
            EEVENEIQPWHGRKPSAVTFLDPHSSS TISDEAESSQSDAESIHDMPRAERKGKKGSC
Sbjct: 181 GEEVENEIQPWHGRKPSAVTFLDPHSSS-TISDEAESSQSDAESIHDMPRAERKGKKGSC 240

Query: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300
           YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT
Sbjct: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300

Query: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360
           KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG
Sbjct: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360

Query: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420
           RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL
Sbjct: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420

Query: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN-- 480
           AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPS SVHSGEE   
Sbjct: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSSSVHSGEEKVD 480

Query: 481 ------------------------------AKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540
                                         AKQIYKVPFSDDERQAIKFLIQRKLYWYIS
Sbjct: 481 KASSSPEPKMLHKLLLVGHDQSGTSTIFKQAKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540

Query: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600
           ILLEGRERFEEE+L+DEKNKQPVNDPSTSSASG+DVQFERKTIYSFGPKLKVFADWLLQV
Sbjct: 541 ILLEGRERFEEEVLLDEKNKQPVNDPSTSSASGNDVQFERKTIYSFGPKLKVFADWLLQV 600

Query: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660
           VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISS 
Sbjct: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSE 660

Query: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720
           DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG
Sbjct: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720

Query: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780
           ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF
Sbjct: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780

Query: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840
           LLILNKFDLFEEKIIQVPLS+CEWFDDFNPMITPNSTGRSSSS++  TNPSLAQRAFQY+
Sbjct: 781 LLILNKFDLFEEKIIQVPLSQCEWFDDFNPMITPNSTGRSSSSTT--TNPSLAQRAFQYV 840

Query: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 873
           AVKFKRLFYSL+DKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEIS+ASAD
Sbjct: 841 AVKFKRLFYSLSDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISTASAD 900

BLAST of Cp4.1LG05g12280 vs. NCBI nr
Match: XP_023007467.1 (extra-large guanine nucleotide-binding protein 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1679 bits (4347), Expect = 0.0
Identity = 854/905 (94.36%), Postives = 863/905 (95.36%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS
Sbjct: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120
           SSQFRDDVS+LPVI+PIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDA KKDNGKDKCD
Sbjct: 61  SSQFRDDVSTLPVIEPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDAIKKDNGKDKCD 120

Query: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180
           IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVR 
Sbjct: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRE 180

Query: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240
           EEEVENEIQPWHGRKPSAVTFLDPH SS TISDEAESSQSDAESIHDMPRAERKGKKGSC
Sbjct: 181 EEEVENEIQPWHGRKPSAVTFLDPHLSS-TISDEAESSQSDAESIHDMPRAERKGKKGSC 240

Query: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300
           YYCLKGNRFTEKEECIVCGAKYCI CVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT
Sbjct: 241 YYCLKGNRFTEKEECIVCGAKYCIVCVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300

Query: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360
           KVLKRLFTDLEVKN L+REKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG
Sbjct: 301 KVLKRLFTDLEVKNILSREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360

Query: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420
           RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL
Sbjct: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420

Query: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN-- 480
           AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPS SVHSGEE   
Sbjct: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSSSVHSGEEKVD 480

Query: 481 ------------------------------AKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540
                                         AKQIYKVPFSDDERQAIKFLIQRKLYWYIS
Sbjct: 481 KASSSPEPKMLHKLLLVGHDQSGTSTIFKQAKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540

Query: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600
           ILLEGRERFEEE+LMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV
Sbjct: 541 ILLEGRERFEEEVLMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600

Query: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660
           VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISS+
Sbjct: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSS 660

Query: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720
           DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG
Sbjct: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720

Query: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780
           ENCKLL+MFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRA SGKNF
Sbjct: 721 ENCKLLEMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRASSGKNF 780

Query: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840
           LLILNKFDLFEEKIIQVPLS+CEWFD+FNPMITPNSTGRSSSSSSS TNPSLAQRAFQYI
Sbjct: 781 LLILNKFDLFEEKIIQVPLSQCEWFDNFNPMITPNSTGRSSSSSSS-TNPSLAQRAFQYI 840

Query: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 873
           AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEIS+ASAD
Sbjct: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISTASAD 900

BLAST of Cp4.1LG05g12280 vs. NCBI nr
Match: XP_023007468.1 (extra-large guanine nucleotide-binding protein 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1612 bits (4175), Expect = 0.0
Identity = 823/875 (94.06%), Postives = 832/875 (95.09%), Query Frame = 0

Query: 31  EYTGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVSSLPVIQPIVGKFSRSSSLKAN 90
           E TGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVS+LPVI+PIVGKFSRSSSLKAN
Sbjct: 4   ERTGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVSTLPVIEPIVGKFSRSSSLKAN 63

Query: 91  LVISSTSEIQEDGPVCLDANKKDNGKDKCDIIESSGELENFNKLKGRLEGGLESLEIKNE 150
           LVISSTSEIQEDGPVCLDA KKDNGKDKCDIIESSGELENFNKLKGRLEGGLESLEIKNE
Sbjct: 64  LVISSTSEIQEDGPVCLDAIKKDNGKDKCDIIESSGELENFNKLKGRLEGGLESLEIKNE 123

Query: 151 EDFQGYTNSSDSESAESGLSSSSGIFAVRGEEEVENEIQPWHGRKPSAVTFLDPHSSSST 210
           EDFQGYTNSSDSESAESGLSSSSGIFAVR EEEVENEIQPWHGRKPSAVTFLDPH SS T
Sbjct: 124 EDFQGYTNSSDSESAESGLSSSSGIFAVREEEEVENEIQPWHGRKPSAVTFLDPHLSS-T 183

Query: 211 ISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIVCGAKYCIACVIRA 270
           ISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIVCGAKYCI CVIRA
Sbjct: 184 ISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIVCGAKYCIVCVIRA 243

Query: 271 MGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKNTLTREKECEINQIPAR 330
           MGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKN L+REKECEINQIPAR
Sbjct: 244 MGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKNILSREKECEINQIPAR 303

Query: 331 LVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRGPCQIVSTHLEVGG 390
           LVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRGPCQIVSTHLEVGG
Sbjct: 304 LVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRGPCQIVSTHLEVGG 363

Query: 391 RIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADGSYQEEGMNNGGKI 450
           RIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADGSYQEEGMNNGGKI
Sbjct: 364 RIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADGSYQEEGMNNGGKI 423

Query: 451 WDKPTTKLACAVFNLPIPSCSVHSGEEN-------------------------------- 510
           WDKPTTKLACAVFNLPIPS SVHSGEE                                 
Sbjct: 424 WDKPTTKLACAVFNLPIPSSSVHSGEEKVDKASSSPEPKMLHKLLLVGHDQSGTSTIFKQ 483

Query: 511 AKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEEILMDEKNKQPVNDPSTSS 570
           AKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEE+LMDEKNKQPVNDPSTSS
Sbjct: 484 AKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEEVLMDEKNKQPVNDPSTSS 543

Query: 571 ASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAF 630
           ASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAF
Sbjct: 544 ASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAF 603

Query: 631 QATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSDNDILYAEGISSCKSLSDMEFSFPE 690
           QATYSRRNELEMLPRVSTYFLDRAVDISS+DYDPSDNDILYAEGISSCKSLSDMEFSFPE
Sbjct: 604 QATYSRRNELEMLPRVSTYFLDRAVDISSSDYDPSDNDILYAEGISSCKSLSDMEFSFPE 663

Query: 691 SRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLDMFEDMSIILFCVALVDYDEFDED 750
           SRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLL+MFEDMSIILFCVALVDYDEFDED
Sbjct: 664 SRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLEMFEDMSIILFCVALVDYDEFDED 723

Query: 751 DNGVLVNRMIASKQLFERIVTDRALSGKNFLLILNKFDLFEEKIIQVPLSKCEWFDDFNP 810
           DNGVLVNRMIASKQLFERIVTDRA SGKNFLLILNKFDLFEEKIIQVPLS+CEWFD+FNP
Sbjct: 724 DNGVLVNRMIASKQLFERIVTDRASSGKNFLLILNKFDLFEEKIIQVPLSQCEWFDNFNP 783

Query: 811 MITPNSTGRSSSSSSSSTNPSLAQRAFQYIAVKFKRLFYSLTDKKLFVSQTTGMEPENVN 870
           MITPNSTGRSSSSSSS TNPSLAQRAFQYIAVKFKRLFYSLTDKKLFVSQTTGMEPENVN
Sbjct: 784 MITPNSTGRSSSSSSS-TNPSLAQRAFQYIAVKFKRLFYSLTDKKLFVSQTTGMEPENVN 843

Query: 871 AALRYAREIIKWEVDKPNISITEISSASADASSFL 873
           AALRYAREIIKWEVDKPNISITEIS+ASADASSFL
Sbjct: 844 AALRYAREIIKWEVDKPNISITEISTASADASSFL 876

BLAST of Cp4.1LG05g12280 vs. ExPASy TrEMBL
Match: A0A6J1G811 (extra-large guanine nucleotide-binding protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451679 PE=4 SV=1)

HSP 1 Score: 1691 bits (4379), Expect = 0.0
Identity = 858/905 (94.81%), Postives = 866/905 (95.69%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS
Sbjct: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120
           SSQFRDDVS+LPVIQPIVG+FSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD
Sbjct: 61  SSQFRDDVSTLPVIQPIVGRFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120

Query: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180
           IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG
Sbjct: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180

Query: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240
            EEVENEIQPWHGRKPSAVTFLDPHSSS TISDEAESSQSDAESIHDMPRAERKGKKGSC
Sbjct: 181 GEEVENEIQPWHGRKPSAVTFLDPHSSS-TISDEAESSQSDAESIHDMPRAERKGKKGSC 240

Query: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300
           YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT
Sbjct: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300

Query: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360
           KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG
Sbjct: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360

Query: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420
           RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL
Sbjct: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420

Query: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN-- 480
           AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPS SVHSGEE   
Sbjct: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSSSVHSGEEKVD 480

Query: 481 ------------------------------AKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540
                                         AKQIYKVPFSDDERQAIKFLIQRKLYWYIS
Sbjct: 481 KASSSPEPKMLHKLLLVGHDQSGTSTIFKQAKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540

Query: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600
           ILLEGRERFEEE+LMDEKNKQPVNDPSTSSASG+DVQFERKTIYSFGPKLKVFADWLLQV
Sbjct: 541 ILLEGRERFEEEVLMDEKNKQPVNDPSTSSASGNDVQFERKTIYSFGPKLKVFADWLLQV 600

Query: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660
           VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISS 
Sbjct: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSE 660

Query: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720
           DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG
Sbjct: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720

Query: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780
           ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF
Sbjct: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780

Query: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840
           LLILNKFDLFEEKIIQVPLS+CEWFDDFNPMITPNSTGRSSSSS+  TNPSLAQRAFQY+
Sbjct: 781 LLILNKFDLFEEKIIQVPLSQCEWFDDFNPMITPNSTGRSSSSST--TNPSLAQRAFQYV 840

Query: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 873
           AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEIS+ASAD
Sbjct: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISTASAD 900

BLAST of Cp4.1LG05g12280 vs. ExPASy TrEMBL
Match: A0A6J1KYT0 (extra-large guanine nucleotide-binding protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499951 PE=4 SV=1)

HSP 1 Score: 1679 bits (4347), Expect = 0.0
Identity = 854/905 (94.36%), Postives = 863/905 (95.36%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS
Sbjct: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGKDKCD 120
           SSQFRDDVS+LPVI+PIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDA KKDNGKDKCD
Sbjct: 61  SSQFRDDVSTLPVIEPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDAIKKDNGKDKCD 120

Query: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRG 180
           IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVR 
Sbjct: 121 IIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVRE 180

Query: 181 EEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSC 240
           EEEVENEIQPWHGRKPSAVTFLDPH SS TISDEAESSQSDAESIHDMPRAERKGKKGSC
Sbjct: 181 EEEVENEIQPWHGRKPSAVTFLDPHLSS-TISDEAESSQSDAESIHDMPRAERKGKKGSC 240

Query: 241 YYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300
           YYCLKGNRFTEKEECIVCGAKYCI CVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT
Sbjct: 241 YYCLKGNRFTEKEECIVCGAKYCIVCVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCT 300

Query: 301 KVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360
           KVLKRLFTDLEVKN L+REKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG
Sbjct: 301 KVLKRLFTDLEVKNILSREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPG 360

Query: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420
           RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL
Sbjct: 361 RYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKL 420

Query: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEEN-- 480
           AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPS SVHSGEE   
Sbjct: 421 AGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSSSVHSGEEKVD 480

Query: 481 ------------------------------AKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540
                                         AKQIYKVPFSDDERQAIKFLIQRKLYWYIS
Sbjct: 481 KASSSPEPKMLHKLLLVGHDQSGTSTIFKQAKQIYKVPFSDDERQAIKFLIQRKLYWYIS 540

Query: 541 ILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600
           ILLEGRERFEEE+LMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV
Sbjct: 541 ILLEGRERFEEEVLMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQV 600

Query: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSA 660
           VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISS+
Sbjct: 601 VVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSS 660

Query: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720
           DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG
Sbjct: 661 DYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLG 720

Query: 721 ENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNF 780
           ENCKLL+MFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRA SGKNF
Sbjct: 721 ENCKLLEMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRASSGKNF 780

Query: 781 LLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYI 840
           LLILNKFDLFEEKIIQVPLS+CEWFD+FNPMITPNSTGRSSSSSSS TNPSLAQRAFQYI
Sbjct: 781 LLILNKFDLFEEKIIQVPLSQCEWFDNFNPMITPNSTGRSSSSSSS-TNPSLAQRAFQYI 840

Query: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASAD 873
           AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEIS+ASAD
Sbjct: 841 AVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISTASAD 900

BLAST of Cp4.1LG05g12280 vs. ExPASy TrEMBL
Match: A0A6J1L7R5 (extra-large guanine nucleotide-binding protein 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499951 PE=4 SV=1)

HSP 1 Score: 1612 bits (4175), Expect = 0.0
Identity = 823/875 (94.06%), Postives = 832/875 (95.09%), Query Frame = 0

Query: 31  EYTGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVSSLPVIQPIVGKFSRSSSLKAN 90
           E TGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVS+LPVI+PIVGKFSRSSSLKAN
Sbjct: 4   ERTGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVSTLPVIEPIVGKFSRSSSLKAN 63

Query: 91  LVISSTSEIQEDGPVCLDANKKDNGKDKCDIIESSGELENFNKLKGRLEGGLESLEIKNE 150
           LVISSTSEIQEDGPVCLDA KKDNGKDKCDIIESSGELENFNKLKGRLEGGLESLEIKNE
Sbjct: 64  LVISSTSEIQEDGPVCLDAIKKDNGKDKCDIIESSGELENFNKLKGRLEGGLESLEIKNE 123

Query: 151 EDFQGYTNSSDSESAESGLSSSSGIFAVRGEEEVENEIQPWHGRKPSAVTFLDPHSSSST 210
           EDFQGYTNSSDSESAESGLSSSSGIFAVR EEEVENEIQPWHGRKPSAVTFLDPH SS T
Sbjct: 124 EDFQGYTNSSDSESAESGLSSSSGIFAVREEEEVENEIQPWHGRKPSAVTFLDPHLSS-T 183

Query: 211 ISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIVCGAKYCIACVIRA 270
           ISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIVCGAKYCI CVIRA
Sbjct: 184 ISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIVCGAKYCIVCVIRA 243

Query: 271 MGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKNTLTREKECEINQIPAR 330
           MGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKN L+REKECEINQIPAR
Sbjct: 244 MGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKNILSREKECEINQIPAR 303

Query: 331 LVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRGPCQIVSTHLEVGG 390
           LVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRGPCQIVSTHLEVGG
Sbjct: 304 LVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRGPCQIVSTHLEVGG 363

Query: 391 RIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADGSYQEEGMNNGGKI 450
           RIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADGSYQEEGMNNGGKI
Sbjct: 364 RIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADGSYQEEGMNNGGKI 423

Query: 451 WDKPTTKLACAVFNLPIPSCSVHSGEEN-------------------------------- 510
           WDKPTTKLACAVFNLPIPS SVHSGEE                                 
Sbjct: 424 WDKPTTKLACAVFNLPIPSSSVHSGEEKVDKASSSPEPKMLHKLLLVGHDQSGTSTIFKQ 483

Query: 511 AKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEEILMDEKNKQPVNDPSTSS 570
           AKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEE+LMDEKNKQPVNDPSTSS
Sbjct: 484 AKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRERFEEEVLMDEKNKQPVNDPSTSS 543

Query: 571 ASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAF 630
           ASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAF
Sbjct: 544 ASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETTFPAATRVYAQSVEELLKDEAF 603

Query: 631 QATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSDNDILYAEGISSCKSLSDMEFSFPE 690
           QATYSRRNELEMLPRVSTYFLDRAVDISS+DYDPSDNDILYAEGISSCKSLSDMEFSFPE
Sbjct: 604 QATYSRRNELEMLPRVSTYFLDRAVDISSSDYDPSDNDILYAEGISSCKSLSDMEFSFPE 663

Query: 691 SRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLDMFEDMSIILFCVALVDYDEFDED 750
           SRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLL+MFEDMSIILFCVALVDYDEFDED
Sbjct: 664 SRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLEMFEDMSIILFCVALVDYDEFDED 723

Query: 751 DNGVLVNRMIASKQLFERIVTDRALSGKNFLLILNKFDLFEEKIIQVPLSKCEWFDDFNP 810
           DNGVLVNRMIASKQLFERIVTDRA SGKNFLLILNKFDLFEEKIIQVPLS+CEWFD+FNP
Sbjct: 724 DNGVLVNRMIASKQLFERIVTDRASSGKNFLLILNKFDLFEEKIIQVPLSQCEWFDNFNP 783

Query: 811 MITPNSTGRSSSSSSSSTNPSLAQRAFQYIAVKFKRLFYSLTDKKLFVSQTTGMEPENVN 870
           MITPNSTGRSSSSSSS TNPSLAQRAFQYIAVKFKRLFYSLTDKKLFVSQTTGMEPENVN
Sbjct: 784 MITPNSTGRSSSSSSS-TNPSLAQRAFQYIAVKFKRLFYSLTDKKLFVSQTTGMEPENVN 843

Query: 871 AALRYAREIIKWEVDKPNISITEISSASADASSFL 873
           AALRYAREIIKWEVDKPNISITEIS+ASADASSFL
Sbjct: 844 AALRYAREIIKWEVDKPNISITEISTASADASSFL 876

BLAST of Cp4.1LG05g12280 vs. ExPASy TrEMBL
Match: A0A5D3CP10 (Extra-large guanine nucleotide-binding protein 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001210 PE=4 SV=1)

HSP 1 Score: 1402 bits (3630), Expect = 0.0
Identity = 718/906 (79.25%), Postives = 779/906 (85.98%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           M G+LKKFFHEKPSS V +EDF  EYSFAIEY GPGINY+IPRAVPIN+DYIPTASVV+S
Sbjct: 1   MAGILKKFFHEKPSSPVNHEDFTGEYSFAIEYKGPGINYEIPRAVPINVDYIPTASVVLS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGK-DKC 120
           SSQF DD+SSLPVIQPIV K  R SS   N VISSTSEIQEDG VCL ANK+D    + C
Sbjct: 61  SSQFSDDLSSLPVIQPIVKKLKRGSSSSPNSVISSTSEIQEDGGVCLHANKEDKCNINSC 120

Query: 121 DIIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVR 180
           D +ESS ELENFN+LKGR+ GG+ESLEIKNEEDFQGY+NSSDSES ESGLSSSSGIFAVR
Sbjct: 121 DGVESSSELENFNELKGRI-GGVESLEIKNEEDFQGYSNSSDSESVESGLSSSSGIFAVR 180

Query: 181 GEEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGS 240
            EEE +NE QP HGR+PSAVTFLDP +S+ TIS+EAESSQ + ESI +MPRAERKGKKGS
Sbjct: 181 EEEEADNETQPRHGRRPSAVTFLDPQTSN-TISEEAESSQFEGESIQEMPRAERKGKKGS 240

Query: 241 CYYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKC 300
           CY+CLKGNRFTEKE C+VCGAKYC  C+IRAMG+MPEGRKCI+CIGFRIDESRR NLGK 
Sbjct: 241 CYFCLKGNRFTEKEVCVVCGAKYCFDCIIRAMGTMPEGRKCISCIGFRIDESRRENLGKS 300

Query: 301 TKVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKP 360
           +KVLK+L TD E+K+ +  EKECEINQ+PARL+Y+N  PLSRQELLMLRSC+KPPKNLKP
Sbjct: 301 SKVLKKLLTDSEIKSIMLHEKECEINQLPARLIYVNGDPLSRQELLMLRSCRKPPKNLKP 360

Query: 361 GRYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILK 420
           G+YWYDKESGFWGKEG GP QIVS+ LEVGGRIKR+ASNGNTNVCINNREITKKELRILK
Sbjct: 361 GQYWYDKESGFWGKEGHGPSQIVSSQLEVGGRIKRNASNGNTNVCINNREITKKELRILK 420

Query: 421 LAGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEE-- 480
           LAGVPCEGRPS+WVSADGSYQEEGMNNGGKIWDK  TKLACA+++LPIPS SV +GEE  
Sbjct: 421 LAGVPCEGRPSFWVSADGSYQEEGMNNGGKIWDKTRTKLACALYSLPIPSNSVRTGEEIE 480

Query: 481 -------------------------------NAKQIYKVPFSDDERQAIKFLIQRKLYWY 540
                                           AKQIYKVPFSDDERQ IKFLIQR LYWY
Sbjct: 481 DGAKSVSSEQKVLHKLLLVGHKKSGTSTIFKQAKQIYKVPFSDDERQMIKFLIQRNLYWY 540

Query: 541 ISILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLL 600
           +SILLEGRERFEEEILMDEK+KQPVNDPS+SSA+G++ Q ERK IYS GPKLK FADWLL
Sbjct: 541 LSILLEGRERFEEEILMDEKSKQPVNDPSSSSAAGNENQLERKDIYSLGPKLKGFADWLL 600

Query: 601 QVVVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDIS 660
           QVVVSG FET FPAATRVY Q VEELLKDEAFQATYSRRNELEMLPRV+TYFLDRA+DIS
Sbjct: 601 QVVVSGNFETIFPAATRVYGQLVEELLKDEAFQATYSRRNELEMLPRVATYFLDRAIDIS 660

Query: 661 SADYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPST 720
           S +YDPSDNDILYAEGI+ C SLS MEF FPESRQDSLLDPPYQHD SIRYQLIRVH ST
Sbjct: 661 SIEYDPSDNDILYAEGITLCNSLSSMEFMFPESRQDSLLDPPYQHDLSIRYQLIRVHSST 720

Query: 721 LGENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGK 780
           LGENCKLL+MF+D+ IILFCV L DYDEFDEDDNGVL NRMIASKQLFE IVT +A  GK
Sbjct: 721 LGENCKLLEMFDDIKIILFCVDLTDYDEFDEDDNGVLTNRMIASKQLFESIVTHQASRGK 780

Query: 781 NFLLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQ 840
           NFLLILNKFDLFEEKIIQVPL++CEWF DFNPMIT    GRSSSS    TNP+LAQRAFQ
Sbjct: 781 NFLLILNKFDLFEEKIIQVPLAQCEWFVDFNPMIT----GRSSSS----TNPTLAQRAFQ 840

Query: 841 YIAVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSAS 872
           YIAVKFKRLF SLTDKKLFVSQTTGMEPENVNAALRYAREIIKW+VDKPNISITE+S  S
Sbjct: 841 YIAVKFKRLFCSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWQVDKPNISITEVSCTS 896

BLAST of Cp4.1LG05g12280 vs. ExPASy TrEMBL
Match: A0A1S3C9U3 (extra-large guanine nucleotide-binding protein 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498424 PE=4 SV=1)

HSP 1 Score: 1402 bits (3630), Expect = 0.0
Identity = 718/906 (79.25%), Postives = 779/906 (85.98%), Query Frame = 0

Query: 1   MDGLLKKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVS 60
           M G+LKKFFHEKPSS V +EDF  EYSFAIEY GPGINY+IPRAVPIN+DYIPTASVV+S
Sbjct: 1   MAGILKKFFHEKPSSPVNHEDFTGEYSFAIEYKGPGINYEIPRAVPINVDYIPTASVVLS 60

Query: 61  SSQFRDDVSSLPVIQPIVGKFSRSSSLKANLVISSTSEIQEDGPVCLDANKKDNGK-DKC 120
           SSQF DD+SSLPVIQPIV K  R SS   N VISSTSEIQEDG VCL ANK+D    + C
Sbjct: 61  SSQFSDDLSSLPVIQPIVKKLKRGSSSSPNSVISSTSEIQEDGGVCLHANKEDKCNINSC 120

Query: 121 DIIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVR 180
           D +ESS ELENFN+LKGR+ GG+ESLEIKNEEDFQGY+NSSDSES ESGLSSSSGIFAVR
Sbjct: 121 DGVESSSELENFNELKGRI-GGVESLEIKNEEDFQGYSNSSDSESVESGLSSSSGIFAVR 180

Query: 181 GEEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGS 240
            EEE +NE QP HGR+PSAVTFLDP +S+ TIS+EAESSQ + ESI +MPRAERKGKKGS
Sbjct: 181 EEEEADNETQPRHGRRPSAVTFLDPQTSN-TISEEAESSQFEGESIQEMPRAERKGKKGS 240

Query: 241 CYYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKC 300
           CY+CLKGNRFTEKE C+VCGAKYC  C+IRAMG+MPEGRKCI+CIGFRIDESRR NLGK 
Sbjct: 241 CYFCLKGNRFTEKEVCVVCGAKYCFDCIIRAMGTMPEGRKCISCIGFRIDESRRENLGKS 300

Query: 301 TKVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKP 360
           +KVLK+L TD E+K+ +  EKECEINQ+PARL+Y+N  PLSRQELLMLRSC+KPPKNLKP
Sbjct: 301 SKVLKKLLTDSEIKSIMLHEKECEINQLPARLIYVNGDPLSRQELLMLRSCRKPPKNLKP 360

Query: 361 GRYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILK 420
           G+YWYDKESGFWGKEG GP QIVS+ LEVGGRIKR+ASNGNTNVCINNREITKKELRILK
Sbjct: 361 GQYWYDKESGFWGKEGHGPSQIVSSQLEVGGRIKRNASNGNTNVCINNREITKKELRILK 420

Query: 421 LAGVPCEGRPSYWVSADGSYQEEGMNNGGKIWDKPTTKLACAVFNLPIPSCSVHSGEE-- 480
           LAGVPCEGRPS+WVSADGSYQEEGMNNGGKIWDK  TKLACA+++LPIPS SV +GEE  
Sbjct: 421 LAGVPCEGRPSFWVSADGSYQEEGMNNGGKIWDKTRTKLACALYSLPIPSNSVRTGEEIE 480

Query: 481 -------------------------------NAKQIYKVPFSDDERQAIKFLIQRKLYWY 540
                                           AKQIYKVPFSDDERQ IKFLIQR LYWY
Sbjct: 481 DGAKSVSSEQKVLHKLLLVGHKKSGTSTIFKQAKQIYKVPFSDDERQMIKFLIQRNLYWY 540

Query: 541 ISILLEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLL 600
           +SILLEGRERFEEEILMDEK+KQPVNDPS+SSA+G++ Q ERK IYS GPKLK FADWLL
Sbjct: 541 LSILLEGRERFEEEILMDEKSKQPVNDPSSSSAAGNENQLERKDIYSLGPKLKGFADWLL 600

Query: 601 QVVVSGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDIS 660
           QVVVSG FET FPAATRVY Q VEELLKDEAFQATYSRRNELEMLPRV+TYFLDRA+DIS
Sbjct: 601 QVVVSGNFETIFPAATRVYGQLVEELLKDEAFQATYSRRNELEMLPRVATYFLDRAIDIS 660

Query: 661 SADYDPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPST 720
           S +YDPSDNDILYAEGI+ C SLS MEF FPESRQDSLLDPPYQHD SIRYQLIRVH ST
Sbjct: 661 SIEYDPSDNDILYAEGITLCNSLSSMEFMFPESRQDSLLDPPYQHDLSIRYQLIRVHSST 720

Query: 721 LGENCKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGK 780
           LGENCKLL+MF+D+ IILFCV L DYDEFDEDDNGVL NRMIASKQLFE IVT +A  GK
Sbjct: 721 LGENCKLLEMFDDIKIILFCVDLTDYDEFDEDDNGVLTNRMIASKQLFESIVTHQASRGK 780

Query: 781 NFLLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQ 840
           NFLLILNKFDLFEEKIIQVPL++CEWF DFNPMIT    GRSSSS    TNP+LAQRAFQ
Sbjct: 781 NFLLILNKFDLFEEKIIQVPLAQCEWFVDFNPMIT----GRSSSS----TNPTLAQRAFQ 840

Query: 841 YIAVKFKRLFYSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSAS 872
           YIAVKFKRLF SLTDKKLFVSQTTGMEPENVNAALRYAREIIKW+VDKPNISITE+S  S
Sbjct: 841 YIAVKFKRLFCSLTDKKLFVSQTTGMEPENVNAALRYAREIIKWQVDKPNISITEVSCTS 896

BLAST of Cp4.1LG05g12280 vs. TAIR 10
Match: AT2G23460.1 (extra-large G-protein 1 )

HSP 1 Score: 726.5 bits (1874), Expect = 2.6e-209
Identity = 417/895 (46.59%), Postives = 549/895 (61.34%), Query Frame = 0

Query: 28  FAIEYTGPGINYDIPRAVPINIDYIPTASVVVSSSQFRDDVSSLPVIQPIVGKFSRSSSL 87
           FA EY GP ++Y+IP AVPIN++ IP A+VV  S     D  S PVIQPI+   S+   +
Sbjct: 11  FAEEYDGPPLSYNIPCAVPINVEKIPVAAVV--SPVCISDNMSFPVIQPILSVESKKFLI 70

Query: 88  KANLVISSTSEIQEDGPVCLDANKKDNGKDKCDIIESSGELENFN-------KLKGRLEG 147
            +   +S TS I   G   L+            +IE + E E          +L    E 
Sbjct: 71  DS---VSPTSVIANCGSNQLELVSDSITVSPTSVIEHTEEEEEEEGGDGEDCELSSSGEL 130

Query: 148 GLESLEIKNEEDFQGYTNS---SDSESAESGLSSSSGIFAVRGEEEVENEIQPWHGRKPS 207
            L S  +K   D    +++    D ES ES LS       V G+   E       G+K  
Sbjct: 131 LLRSCSVKESLDLNESSSNPLVPDWESNESVLSMDYPSSRVTGDCVSETN---GDGKKQP 190

Query: 208 AVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGSCYYCLKGNRFTEKEECIV 267
            VTFL   S      +E+ S+      +    + + KGKKGSCY C KG+RFTEKE C+V
Sbjct: 191 VVTFLGIASDDGFEEEESCSNLRRVRVVPVKKQPQTKGKKGSCYRCFKGSRFTEKEVCLV 250

Query: 268 CGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLEVKNTLT 327
           C AKYC +CV+RAMGSMPEGRKC+TCIGF IDES+R +LGKC+++LKRL  DLEVK  + 
Sbjct: 251 CDAKYCNSCVLRAMGSMPEGRKCVTCIGFPIDESKRGSLGKCSRMLKRLLNDLEVKQIMK 310

Query: 328 REKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFWGKEGRG 387
            E+ CE NQ+PA  VY+N  PL  +EL+ L++C  PPK LKPG YWYDK SG WGKEG  
Sbjct: 311 TERFCEANQLPAEYVYVNGQPLYPEELVTLQTCSNPPKKLKPGDYWYDKVSGLWGKEGEK 370

Query: 388 PCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSYWVSADG 447
           P QI+S HL VGG I  +ASNGNT V IN REITK ELR+L+LAGV C G P +WV+ DG
Sbjct: 371 PYQIISPHLNVGGPISPEASNGNTQVFINGREITKVELRMLQLAGVQCAGNPHFWVNEDG 430

Query: 448 SYQEEGM-NNGGKIWDKPTTKLACAVFNLPIPSCSV--HSGEE----------------- 507
           SYQEEG  N  G IW K  TKL CAV +LP+PS S    SGE+                 
Sbjct: 431 SYQEEGQKNTKGYIWGKAGTKLLCAVLSLPVPSKSTANASGEQLYSANSRSILDHLEHRT 490

Query: 508 -------------------NAKQIYK-VPFSDDERQAIKFLIQRKLYWYISILLEGRERF 567
                               AK +YK VPF +DER+ IK +IQ  +Y Y+ +LLEGRERF
Sbjct: 491 LQKILLVGNSGSGTSTIFKQAKILYKDVPFLEDERENIKVIIQTNVYGYLGMLLEGRERF 550

Query: 568 EEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYFETT 627
           EEE L     KQ V +   +    +    +  T+YS GP+LK F+DWLL+ + +G     
Sbjct: 551 EEEALALRNTKQCVLENIPADEGDAKSNDKTVTMYSIGPRLKAFSDWLLKTMAAGNLGVI 610

Query: 628 FPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSDNDI 687
           FPAA+R YA  VEEL +D A QATY RR+EL +LP V++YFL+RA+D+ + DY+PSD DI
Sbjct: 611 FPAASREYAPLVEELWRDAAIQATYKRRSELGLLPSVASYFLERAIDVLTPDYEPSDLDI 670

Query: 688 LYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLGENCKLLDMF 747
           LYAEG++S   L+ ++FSFP++  +  LDP   HDS +RYQLIRV    LGENCK +DMF
Sbjct: 671 LYAEGVTSSSGLACLDFSFPQTASEENLDPSDHHDSLLRYQLIRVPSRGLGENCKWIDMF 730

Query: 748 EDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNFLLILNKFDL 807
           ED+ +++F V++ DYD+  ED      N+M+ +K+LFE I+T       +FLLILNK+DL
Sbjct: 731 EDVGMVVFVVSMSDYDQVSEDG----TNKMLLTKKLFESIITHPIFENMDFLLILNKYDL 790

Query: 808 FEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYIAVKFKRLFY 867
            EEK+ +VPL++CEWF DFNP++       S    S++ NP+L Q AF ++AVKFKR + 
Sbjct: 791 LEEKVERVPLARCEWFQDFNPVV-------SRHRGSNNGNPTLGQLAFHFMAVKFKRFYS 850

Query: 868 SLTDKKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISITEISSASADASSF 873
           SLT KKLFVS +  ++P +V+++L+ A EI+KW  ++ NI ++E S  S + SSF
Sbjct: 851 SLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNICMSEYSMYSTEPSSF 886

BLAST of Cp4.1LG05g12280 vs. TAIR 10
Match: AT4G34390.1 (extra-large GTP-binding protein 2 )

HSP 1 Score: 661.0 bits (1704), Expect = 1.3e-189
Identity = 398/911 (43.69%), Postives = 546/911 (59.93%), Query Frame = 0

Query: 6   KKFFHEKPSSSVGNEDFNPEYSFAIEYTGPGINYDIPRAVPINIDYIPTASVVVSSSQFR 65
           K+   E       +  +  EYSFA EY GP I  ++PRA+P+ +D IPTA + VS S  R
Sbjct: 17  KRDNRESDDDDETSSGYRIEYSFASEYKGPLI-ANVPRALPVEVDQIPTA-LPVSFSSLR 76

Query: 66  DDVSSLPVIQPIVGKFSR----SSSLKANLVISST--SEIQEDGPVCLDANKKDNGKDKC 125
             + S PV   ++ K ++    S   K N  + S   S +   G   +  +   +   + 
Sbjct: 77  SGI-SYPVAPLVMTKDTKRPPDSGIEKKNGFVDSAAGSSVVLIGRDVVSGSSSSSSSKRL 136

Query: 126 DIIESSGELENFNKLKGRLEGGLESLEIKNEEDFQGYTNSSDSESAESGLSSSSGIFAVR 185
           D+ E                      E+K+  DF+         S  S LS+S+     R
Sbjct: 137 DVPE----------------------EVKSPADFR--------LSPSSPLSASA-----R 196

Query: 186 GEEEVENEIQPWHGRKPSAVTFLDPHSSSSTISDEAESSQSDAESIHDMPRAERKGKKGS 245
            E+ ++++     G  P AV F++P  SS     +  S  SD ESI    RAERKGK+GS
Sbjct: 197 EEDHLDDDRVSDVG--PRAVRFVEPFQSSEC---DESSYVSDGESIAATHRAERKGKRGS 256

Query: 246 CYYCLKGNRFTEKEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKC 305
           CY C  GNRFTEKE CIVC AKYC  CV RAMG+MPEGRKC  CIG+RIDES+R +LGKC
Sbjct: 257 CYRCQLGNRFTEKEVCIVCDAKYCFNCVRRAMGAMPEGRKCQACIGYRIDESKRASLGKC 316

Query: 306 TKVLKRLFTDLEVKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKP 365
           +++LKR  TD E++  +  E  C+ NQ+P+RL+ +ND PLS  EL  L++C  PPK LKP
Sbjct: 317 SRMLKRHLTDSELRQVMNAEITCKANQLPSRLIIVNDKPLSEDELYTLQTCPNPPKKLKP 376

Query: 366 GRYWYDKESGFWGKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILK 425
           G YWYDK +G+WGK G  P QI+S +  +GG I    SNG+T + IN REITK EL +LK
Sbjct: 377 GHYWYDKVAGYWGKIGEKPSQIISPNNSIGGYISEKVSNGDTEIYINGREITKPELTMLK 436

Query: 426 LAGVPCEGRPSYWVSADGSYQEEGMNNG-GKIWDKPTTKLACAVFNLPIPSCS------- 485
            AGV CEG+P +WV +DGSY+EEG  +  G IW K   K+ACAVF+LP+P  S       
Sbjct: 437 WAGVQCEGKPHFWVDSDGSYREEGQKHPIGNIWSKKRAKIACAVFSLPVPPASSAVEPYD 496

Query: 486 ------------VHSGEE---------NAKQIYKVPFSDDERQAIKFLIQRKLYWYISIL 545
                       +  G E          A+ +Y V FS ++R+ IKF+IQ  LY Y++++
Sbjct: 497 VPLYEQKMLNKLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMV 556

Query: 546 LEGRERFEEEILMDEKNKQPVNDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVV 605
           LE  ERFE+E+  D+ +   V D  TS+  G+          S  P+LK F+DW+L+   
Sbjct: 557 LEAHERFEKEMSNDQSSGN-VGD-ETSAKPGN----------SINPRLKHFSDWVLKEKE 616

Query: 606 SGYFETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADY 665
            G  +  FP ++R  AQ+V +L +  A QATY R    + LPR + YFL+R ++IS ++Y
Sbjct: 617 DGNLK-IFPPSSRENAQTVADLWRVPAIQATYKRLR--DTLPRNAVYFLERILEISRSEY 676

Query: 666 DPSDNDILYAEGISSCKSLSDMEFSFPESRQDSLLDPPYQHDSSIRYQLIRVHPSTLGEN 725
           DPSD DIL AEG+SS + LS ++FSFP + Q+  L+  YQHD+ ++YQLIR++P +LGEN
Sbjct: 677 DPSDMDILQAEGLSSMEGLSCVDFSFPSTSQEESLESDYQHDTDMKYQLIRLNPRSLGEN 736

Query: 726 CKLLDMFEDMSIILFCVALVDYDEFDEDDNGVLVNRMIASKQLFERIVTDRALSGKNFLL 785
            KLL+MFED  +++FCV+L DY E  ED  G +VN+M+A+KQLFE +VT  +L+ K FLL
Sbjct: 737 WKLLEMFEDADLVIFCVSLTDYAENIEDGEGNIVNKMLATKQLFENMVTHPSLANKRFLL 796

Query: 786 ILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQYIAV 845
           +L KFDL EEKI +VPL  CEWF+DFNP+I+ N T R         NP +AQRAF YI  
Sbjct: 797 VLTKFDLLEEKIEEVPLRTCEWFEDFNPLISQNQTSRH--------NPPMAQRAFHYIGY 856

Query: 846 KFKRLFYSLTD----------KKLFVSQTTGMEPENVNAALRYAREIIKWEVDKPNISIT 872
           KFKRL+ S+ +           KLFV Q + +E + V+ ALRYAREI+KW V++ ++   
Sbjct: 857 KFKRLYDSILEPVNMRGRSFKPKLFVCQVS-LESDTVDNALRYAREILKWHVEETSM-FQ 859

BLAST of Cp4.1LG05g12280 vs. TAIR 10
Match: AT1G31930.1 (extra-large GTP-binding protein 3 )

HSP 1 Score: 541.2 bits (1393), Expect = 1.5e-153
Identity = 323/787 (41.04%), Postives = 453/787 (57.56%), Query Frame = 0

Query: 155 GYTNSS-----DSESAESGL-------SSSSGIFAVRGEEEVENEIQPWHGRKPSAVTF- 214
           G T+SS      SES  S L       S S+ +  V G  +  N++     R+P  V F 
Sbjct: 74  GVTSSSGGSPASSESVVSVLHNNPESSSGSASVSPVSGHRQNGNQV-----RRP-VVKFK 133

Query: 215 -LDPHSS-SSTISDEAESSQSDAES-----IHD---MPRAERKGKKGSCYYCLKGNRFTE 274
            +D H       + E E +  +AE+     +H+     +  +K KK  CY C K  ++  
Sbjct: 134 PVDDHDRIEGREAAEEEDNNVEAETERERKVHECTASTKRRKKKKKSECYRCGKA-KWEN 193

Query: 275 KEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLE 334
           KE CIVC  KYC  CV+RAMGSMPEGRKC++CIG  IDES+R+ LGK ++VL RL + LE
Sbjct: 194 KETCIVCDEKYCGNCVLRAMGSMPEGRKCVSCIGQAIDESKRSKLGKHSRVLSRLLSPLE 253

Query: 335 VKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFW 394
           VK  +  EKEC  NQ+    + +N +PL  +E+  L +C  PP+ LKPGRYWYDKESG W
Sbjct: 254 VKQIMKAEKECTANQLRPEQLIVNGYPLKPEEMADLLNCLLPPQKLKPGRYWYDKESGLW 313

Query: 395 GKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSY 454
           GKEG  P +++S++L   G++  DASNGNT V IN REITK ELRILKLA V C     +
Sbjct: 314 GKEGEKPDRVISSNLNFTGKLSPDASNGNTEVYINGREITKLELRILKLANVQCPRDTHF 373

Query: 455 WVSADGSYQEEGMNN-GGKIWDKPTTKLACAVFNLPIPSCSVH----------------- 514
           WV  DG Y+EEG NN  G IW+K +T+  CA+F+LP+P                      
Sbjct: 374 WVYDDGRYEEEGQNNIRGNIWEKASTRFMCALFSLPVPQGQPRGTVQPSSNYATVPNYIE 433

Query: 515 --------------SGE----ENAKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRE 574
                         SG     + AK +Y   FS +E Q IK ++Q  +Y Y+SILL+GRE
Sbjct: 434 HKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRE 493

Query: 575 RFEEEILMDEKNKQPV-NDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYF 634
           RFEEE L   +    V  D     A+        +++Y+  P+LK F+DWLL ++ +G  
Sbjct: 494 RFEEEALSHTRGLNAVEGDSGGEEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDL 553

Query: 635 ETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSD 694
           +  FPAATR YA  VEE+ KD A QATY R++EL  LP V+ YFL RA+++SS +Y+PS+
Sbjct: 554 DAFFPAATREYAPLVEEVWKDPAIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSE 613

Query: 695 NDILYAEGISSCKSLSDMEFSFPESRQDSLL---DPPYQHDSSIRYQLIRVHPSTLGENC 754
            DI+YAEG++    L+ MEFS  +    S     +P        +YQLIRV+   + ++C
Sbjct: 614 RDIVYAEGVTQGNGLAFMEFSLSDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSC 673

Query: 755 KLLDMFEDMSIILFCVALVDYDEFD--EDDNGVL--VNRMIASKQLFERIVTDRALSGKN 814
           K ++MFED+  ++FC++L DYD+ +   + +G +   N+MI SK+LFE +V         
Sbjct: 674 KWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTP 733

Query: 815 FLLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQY 872
           F+LILNK+D FEEK+ + PL+ C+WF DF P+ T N+              SLA +A+ Y
Sbjct: 734 FILILNKYDQFEEKLNRAPLTSCDWFSDFCPVRTNNNV------------QSLAYQAYFY 793

BLAST of Cp4.1LG05g12280 vs. TAIR 10
Match: AT1G31930.2 (extra-large GTP-binding protein 3 )

HSP 1 Score: 541.2 bits (1393), Expect = 1.5e-153
Identity = 323/787 (41.04%), Postives = 453/787 (57.56%), Query Frame = 0

Query: 155 GYTNSS-----DSESAESGL-------SSSSGIFAVRGEEEVENEIQPWHGRKPSAVTF- 214
           G T+SS      SES  S L       S S+ +  V G  +  N++     R+P  V F 
Sbjct: 74  GVTSSSGGSPASSESVVSVLHNNPESSSGSASVSPVSGHRQNGNQV-----RRP-VVKFK 133

Query: 215 -LDPHSS-SSTISDEAESSQSDAES-----IHD---MPRAERKGKKGSCYYCLKGNRFTE 274
            +D H       + E E +  +AE+     +H+     +  +K KK  CY C K  ++  
Sbjct: 134 PVDDHDRIEGREAAEEEDNNVEAETERERKVHECTASTKRRKKKKKSECYRCGKA-KWEN 193

Query: 275 KEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLE 334
           KE CIVC  KYC  CV+RAMGSMPEGRKC++CIG  IDES+R+ LGK ++VL RL + LE
Sbjct: 194 KETCIVCDEKYCGNCVLRAMGSMPEGRKCVSCIGQAIDESKRSKLGKHSRVLSRLLSPLE 253

Query: 335 VKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFW 394
           VK  +  EKEC  NQ+    + +N +PL  +E+  L +C  PP+ LKPGRYWYDKESG W
Sbjct: 254 VKQIMKAEKECTANQLRPEQLIVNGYPLKPEEMADLLNCLLPPQKLKPGRYWYDKESGLW 313

Query: 395 GKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSY 454
           GKEG  P +++S++L   G++  DASNGNT V IN REITK ELRILKLA V C     +
Sbjct: 314 GKEGEKPDRVISSNLNFTGKLSPDASNGNTEVYINGREITKLELRILKLANVQCPRDTHF 373

Query: 455 WVSADGSYQEEGMNN-GGKIWDKPTTKLACAVFNLPIPSCSVH----------------- 514
           WV  DG Y+EEG NN  G IW+K +T+  CA+F+LP+P                      
Sbjct: 374 WVYDDGRYEEEGQNNIRGNIWEKASTRFMCALFSLPVPQGQPRGTVQPSSNYATVPNYIE 433

Query: 515 --------------SGE----ENAKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRE 574
                         SG     + AK +Y   FS +E Q IK ++Q  +Y Y+SILL+GRE
Sbjct: 434 HKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRE 493

Query: 575 RFEEEILMDEKNKQPV-NDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYF 634
           RFEEE L   +    V  D     A+        +++Y+  P+LK F+DWLL ++ +G  
Sbjct: 494 RFEEEALSHTRGLNAVEGDSGGEEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDL 553

Query: 635 ETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSD 694
           +  FPAATR YA  VEE+ KD A QATY R++EL  LP V+ YFL RA+++SS +Y+PS+
Sbjct: 554 DAFFPAATREYAPLVEEVWKDPAIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSE 613

Query: 695 NDILYAEGISSCKSLSDMEFSFPESRQDSLL---DPPYQHDSSIRYQLIRVHPSTLGENC 754
            DI+YAEG++    L+ MEFS  +    S     +P        +YQLIRV+   + ++C
Sbjct: 614 RDIVYAEGVTQGNGLAFMEFSLSDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSC 673

Query: 755 KLLDMFEDMSIILFCVALVDYDEFD--EDDNGVL--VNRMIASKQLFERIVTDRALSGKN 814
           K ++MFED+  ++FC++L DYD+ +   + +G +   N+MI SK+LFE +V         
Sbjct: 674 KWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTP 733

Query: 815 FLLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQY 872
           F+LILNK+D FEEK+ + PL+ C+WF DF P+ T N+              SLA +A+ Y
Sbjct: 734 FILILNKYDQFEEKLNRAPLTSCDWFSDFCPVRTNNNV------------QSLAYQAYFY 793

BLAST of Cp4.1LG05g12280 vs. TAIR 10
Match: AT1G31930.3 (extra-large GTP-binding protein 3 )

HSP 1 Score: 541.2 bits (1393), Expect = 1.5e-153
Identity = 323/787 (41.04%), Postives = 453/787 (57.56%), Query Frame = 0

Query: 155 GYTNSS-----DSESAESGL-------SSSSGIFAVRGEEEVENEIQPWHGRKPSAVTF- 214
           G T+SS      SES  S L       S S+ +  V G  +  N++     R+P  V F 
Sbjct: 74  GVTSSSGGSPASSESVVSVLHNNPESSSGSASVSPVSGHRQNGNQV-----RRP-VVKFK 133

Query: 215 -LDPHSS-SSTISDEAESSQSDAES-----IHD---MPRAERKGKKGSCYYCLKGNRFTE 274
            +D H       + E E +  +AE+     +H+     +  +K KK  CY C K  ++  
Sbjct: 134 PVDDHDRIEGREAAEEEDNNVEAETERERKVHECTASTKRRKKKKKSECYRCGKA-KWEN 193

Query: 275 KEECIVCGAKYCIACVIRAMGSMPEGRKCITCIGFRIDESRRNNLGKCTKVLKRLFTDLE 334
           KE CIVC  KYC  CV+RAMGSMPEGRKC++CIG  IDES+R+ LGK ++VL RL + LE
Sbjct: 194 KETCIVCDEKYCGNCVLRAMGSMPEGRKCVSCIGQAIDESKRSKLGKHSRVLSRLLSPLE 253

Query: 335 VKNTLTREKECEINQIPARLVYINDHPLSRQELLMLRSCQKPPKNLKPGRYWYDKESGFW 394
           VK  +  EKEC  NQ+    + +N +PL  +E+  L +C  PP+ LKPGRYWYDKESG W
Sbjct: 254 VKQIMKAEKECTANQLRPEQLIVNGYPLKPEEMADLLNCLLPPQKLKPGRYWYDKESGLW 313

Query: 395 GKEGRGPCQIVSTHLEVGGRIKRDASNGNTNVCINNREITKKELRILKLAGVPCEGRPSY 454
           GKEG  P +++S++L   G++  DASNGNT V IN REITK ELRILKLA V C     +
Sbjct: 314 GKEGEKPDRVISSNLNFTGKLSPDASNGNTEVYINGREITKLELRILKLANVQCPRDTHF 373

Query: 455 WVSADGSYQEEGMNN-GGKIWDKPTTKLACAVFNLPIPSCSVH----------------- 514
           WV  DG Y+EEG NN  G IW+K +T+  CA+F+LP+P                      
Sbjct: 374 WVYDDGRYEEEGQNNIRGNIWEKASTRFMCALFSLPVPQGQPRGTVQPSSNYATVPNYIE 433

Query: 515 --------------SGE----ENAKQIYKVPFSDDERQAIKFLIQRKLYWYISILLEGRE 574
                         SG     + AK +Y   FS +E Q IK ++Q  +Y Y+SILL+GRE
Sbjct: 434 HKKIQKLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRE 493

Query: 575 RFEEEILMDEKNKQPV-NDPSTSSASGSDVQFERKTIYSFGPKLKVFADWLLQVVVSGYF 634
           RFEEE L   +    V  D     A+        +++Y+  P+LK F+DWLL ++ +G  
Sbjct: 494 RFEEEALSHTRGLNAVEGDSGGEEANDEGTVTTPQSVYTLNPRLKHFSDWLLDIIATGDL 553

Query: 635 ETTFPAATRVYAQSVEELLKDEAFQATYSRRNELEMLPRVSTYFLDRAVDISSADYDPSD 694
           +  FPAATR YA  VEE+ KD A QATY R++EL  LP V+ YFL RA+++SS +Y+PS+
Sbjct: 554 DAFFPAATREYAPLVEEVWKDPAIQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSE 613

Query: 695 NDILYAEGISSCKSLSDMEFSFPESRQDSLL---DPPYQHDSSIRYQLIRVHPSTLGENC 754
            DI+YAEG++    L+ MEFS  +    S     +P        +YQLIRV+   + ++C
Sbjct: 614 RDIVYAEGVTQGNGLAFMEFSLSDHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSC 673

Query: 755 KLLDMFEDMSIILFCVALVDYDEFD--EDDNGVL--VNRMIASKQLFERIVTDRALSGKN 814
           K ++MFED+  ++FC++L DYD+ +   + +G +   N+MI SK+LFE +V         
Sbjct: 674 KWVEMFEDVRAVIFCISLSDYDQINITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTP 733

Query: 815 FLLILNKFDLFEEKIIQVPLSKCEWFDDFNPMITPNSTGRSSSSSSSSTNPSLAQRAFQY 872
           F+LILNK+D FEEK+ + PL+ C+WF DF P+ T N+              SLA +A+ Y
Sbjct: 734 FILILNKYDQFEEKLNRAPLTSCDWFSDFCPVRTNNNV------------QSLAYQAYFY 793