Homology
BLAST of Cp4.1LG05g11730 vs. ExPASy Swiss-Prot
Match:
Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)
HSP 1 Score: 163.7 bits (413), Expect = 5.1e-39
Identity = 140/468 (29.91%), Postives = 221/468 (47.22%), Query Frame = 0
Query: 46 NSSRFISNPRRHLRDFPNSNNRSNARMRLYDDLLLN--------GYYTTKLWIGTPPQQF 105
N + + + L + + ++ +ARM DL L G Y TK+ +G+PP+++
Sbjct: 32 NVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDSRADSIGLYFTKIKLGSPPKEY 91
Query: 106 ALIVDTGSTVTYVPCSTCVECGKHQD-----PKFDPESSSTYQAVECNSD-----CNCDN 165
+ VDTGS + +V C+ C +C D +D ++SST + V C D +
Sbjct: 92 YVQVDTGSDILWVNCAPCPKCPVKTDLGIPLSLYDSKTSSTSKNVGCEDDFCSFIMQSET 151
Query: 166 DGLQ--CIYERKYAEMSTSSGVLGEDVISF----GN-QSELIPQRAVFGCENEETGDL-- 225
G + C Y Y + STS G +D I+ GN ++ + Q VFGC ++G L
Sbjct: 152 CGAKKPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQ 211
Query: 226 YSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIGGGAMVLGGISPPSDMIFS 285
DGIMG G+ + SI+ QL G FS C M+ GGG +G + P ++ +
Sbjct: 212 TDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMN-GGGIFAVGEVESP--VVKT 271
Query: 286 YSDPVRSPYYNVDLKGIHVAGKKLGLSPSV--FDGRYGTVLDSGTTYAYLPEEAFGAFKD 345
+YNV LKG+ V G + L PS+ +G GT++DSGTT AYLP+ + + +
Sbjct: 272 TPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIE 331
Query: 346 AI----MNELHSLKKIDGPDPNFKDTCFS-SAGSDAADLSDIFPTVDMIFDHEQKLSLAP 405
I +LH +++ CFS ++ +D A FP V++ F+ KLS+ P
Sbjct: 332 KITAKQQVKLHMVQE--------TFACFSFTSNTDKA-----FPVVNLHFEDSLKLSVYP 391
Query: 406 ENYLFPHSKIHGAYCLGIFGNANDQTT-------LLGGIVVRNTLVMYDRKHSKVGFWKT 465
+YLF S YC G + TT LLG +V+ N LV+YD ++ +G+
Sbjct: 392 HDYLF--SLREDMYCFG--WQSGGMTTQDGADVILLGDLVLSNKLVVYDLENEVIGWADH 451
Query: 466 NCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIFV 473
NCS + + D + A + I+ AS+ + I I+V
Sbjct: 452 NCS---SSIKVKDGSGAAYQLGAENLISAASSVMNGTLVTLLSILIWV 476
BLAST of Cp4.1LG05g11730 vs. ExPASy Swiss-Prot
Match:
Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)
HSP 1 Score: 157.1 bits (396), Expect = 4.8e-37
Identity = 119/405 (29.38%), Postives = 196/405 (48.40%), Query Frame = 0
Query: 55 RRHLRDFPNSNNRSNARMRLYDDLLLN--------GYYTTKLWIGTPPQQFALIVDTGST 114
+++L F + + R ++RM DL L G Y TK+ +G+PP+++ + VDTGS
Sbjct: 37 KKNLEHFKSHDTRRHSRMLASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSD 96
Query: 115 VTYVPCSTCVECGKHQD-----PKFDPESSSTYQAVECNSD-CN--CDNDGLQ----CIY 174
+ ++ C C +C + FD +SST + V C+ D C+ +D Q C Y
Sbjct: 97 ILWINCKPCPKCPTKTNLNFRLSLFDMNASSTSKKVGCDDDFCSFISQSDSCQPALGCSY 156
Query: 175 ERKYAEMSTSSGVLGEDVISFGN-----QSELIPQRAVFGCENEETGDLYS--QRADGIM 234
YA+ STS G D+++ ++ + Q VFGC ++++G L + DG+M
Sbjct: 157 HIVYADESTSDGKFIRDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVM 216
Query: 235 GLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIGGGAMVLGGISPPSDMIFSYSDPVRSPY 294
G G+ + S++ QL G FS C + GGG +G + P + + +
Sbjct: 217 GFGQSNTSVLSQLAATGDAKRVFSHCLDNVK-GGGIFAVGVVDSPK--VKTTPMVPNQMH 276
Query: 295 YNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSGTTYAYLPEEAFGAFKDAIMNELHSLKK 354
YNV L G+ V G L L S+ GT++DSGTT AY P+ + + + I+ K
Sbjct: 277 YNVMLMGMDVDGTSLDLPRSIVRNG-GTIVDSGTTLAYFPKVLYDSLIETIL--ARQPVK 336
Query: 355 IDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMIFDHEQKLSLAPENYLFPHSKIHGAYCL 414
+ + F+ CFS + ++ + FP V F+ KL++ P +YLF + YC
Sbjct: 337 LHIVEETFQ--CFSF----STNVDEAFPPVSFEFEDSVKLTVYPHDYLFTLEE--ELYCF 396
Query: 415 -----GIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVGFWKTNCS 428
G+ + + LLG +V+ N LV+YD + +G+ NCS
Sbjct: 397 GWQAGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCS 427
BLAST of Cp4.1LG05g11730 vs. ExPASy Swiss-Prot
Match:
Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)
HSP 1 Score: 156.4 bits (394), Expect = 8.2e-37
Identity = 108/364 (29.67%), Postives = 180/364 (49.45%), Query Frame = 0
Query: 81 NGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPKFDPESSSTYQAVECN 140
+G Y +++ +GTP ++ L++DTGS V ++ C C +C + DP F+P SSSTY+++ C+
Sbjct: 159 SGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCS 218
Query: 141 S-DCN------CDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSELIPQRAVFGCENE 200
+ C+ C ++ +C+Y+ Y + S + G L D ++FGN ++ GC ++
Sbjct: 219 APQCSLLETSACRSN--KCLYQVSYGDGSFTVGELATDTVTFGNSGKI--NNVALGCGHD 278
Query: 201 ETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIGGGAMV------LG 260
G L++ A G++GLG G LSI +Q+ SFS C D G + + LG
Sbjct: 279 NEG-LFTGAA-GLLGLGGGVLSITNQMK-----ATSFSYCLVDRDSGKSSSLDFNSVQLG 338
Query: 261 GISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFD----GRYGTVLDSGTTY 320
G + ++ + +Y V L G V G+K+ L ++FD G G +LD GT
Sbjct: 339 GGDATAPLL---RNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAV 398
Query: 321 AYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDI-FPTVDMIFD 380
L +A+ + +DA + +LKK F DTC+ D + LS + PTV F
Sbjct: 399 TRLQTQAYNSLRDAFLKLTVNLKKGSSSISLF-DTCY-----DFSSLSTVKVPTVAFHFT 458
Query: 381 HEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVGFW 427
+ L L +NYL P G +C F + +++G + + T + YD + +G
Sbjct: 459 GGKSLDLPAKNYLIPVDD-SGTFCFA-FAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLS 500
BLAST of Cp4.1LG05g11730 vs. ExPASy Swiss-Prot
Match:
Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)
HSP 1 Score: 151.4 bits (381), Expect = 2.6e-35
Identity = 116/365 (31.78%), Postives = 175/365 (47.95%), Query Frame = 0
Query: 81 NGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPKFDPESSSTYQAVECN 140
+G Y + IGTP F+ I+DTGS + + C C +C P F+P+ SS++ + C
Sbjct: 93 DGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCE 152
Query: 141 SD-C------NCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSELIPQRAVFGCENE 200
S C C+N+ +C Y Y + ST+ G + + +F S +P A FGC +
Sbjct: 153 SQYCQDLPSETCNNN--ECQYTYGYGDGSTTQGYMATETFTFETSS--VPNIA-FGCGED 212
Query: 201 ETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLC---YGG-----MDIGGGAMV 260
G A G++G+G G LS+ QL GV FS C YG + +G A
Sbjct: 213 NQGFGQGNGA-GLIGMGWGPLSLPSQL---GV--GQFSYCMTSYGSSSPSTLALGSAASG 272
Query: 261 LGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVF----DGRYGTVLDSGT 320
+ SP + +I S +P YY + L+GI V G LG+ S F DG G ++DSGT
Sbjct: 273 VPEGSPSTTLIHSSLNPT---YYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGT 332
Query: 321 TYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMIF 380
T YLP++A+ A A +++ +L +D TCF SD + + P + M F
Sbjct: 333 TLTYLPQDAYNAVAQAFTDQI-NLPTVDESSSGL-STCFQQP-SDGSTVQ--VPEISMQF 392
Query: 381 DHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVGF 427
D L+L +N L S G CL + ++ ++ G I + T V+YD ++ V F
Sbjct: 393 D-GGVLNLGEQNILI--SPAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSF 435
BLAST of Cp4.1LG05g11730 vs. ExPASy Swiss-Prot
Match:
Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)
HSP 1 Score: 150.6 bits (379), Expect = 4.5e-35
Identity = 111/362 (30.66%), Postives = 168/362 (46.41%), Query Frame = 0
Query: 81 NGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPKFDPESSSTYQAVECN 140
+G Y ++ +G+PP+ +++D+GS + +V C C C K DP FDP S +Y V C
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187
Query: 141 SDCNCD---NDGLQ---CIYERKYAEMSTSSGVLGEDVISFGNQSELIPQRAVFGCENEE 200
S CD N G C YE Y + S + G L + ++F ++ + + GC +
Sbjct: 188 SSV-CDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF---AKTVVRNVAMGCGHRN 247
Query: 201 TGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCY--GGMDIGGGAMVLGGISPP 260
G A G++G+G G +S V QL G +F C G D G++V G + P
Sbjct: 248 RGMFIG--AAGLLGIGGGSMSFVGQL--SGQTGGAFGYCLVSRGTD-STGSLVFGREALP 307
Query: 261 SDMIFSYSDPVRSP----YYNVDLKGIHVAGKKLGLSPSVFD----GRYGTVLDSGTTYA 320
S+ VR+P +Y V LKG+ V G ++ L VFD G G V+D+GT
Sbjct: 308 VGA--SWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVT 367
Query: 321 YLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMIFDHE 380
LP A+ AF+D ++ +L + G + DTC+ +G +S PTV F
Sbjct: 368 RLPTAAYVAFRDGFKSQTANLPRASG--VSIFDTCYDLSGF----VSVRVPTVSFYFTEG 427
Query: 381 QKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVGFWKT 427
L+L N+L P G YC F + +++G I V +D + VGF
Sbjct: 428 PVLTLPARNFLMPVDD-SGTYCFA-FAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPN 470
BLAST of Cp4.1LG05g11730 vs. NCBI nr
Match:
KAG7035174.1 (Aspartic proteinase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 466/475 (98.11%), Postives = 472/475 (99.37%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPRR LRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPRRRLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDP+FDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPRFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFSS+GSDAA+LSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSSSGSDAANLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIFVMLL 475
FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIF FVMLL
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFSFVMLL 475
BLAST of Cp4.1LG05g11730 vs. NCBI nr
Match:
XP_023007338.1 (aspartic proteinase nepenthesin-2-like isoform X5 [Cucurbita maxima])
HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 461/479 (96.24%), Postives = 469/479 (97.91%), Query Frame = 0
Query: 1 MVRTLILL-----HFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPR 60
MVRTLILL H LHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPR
Sbjct: 1 MVRTLILLPAILIHCLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPR 60
Query: 61 RHLRDFPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC 120
RHLRDF NSNNRSNARMRLYD+LLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC
Sbjct: 61 RHLRDFTNSNNRSNARMRLYDELLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC 120
Query: 121 VECGKHQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF 180
VECGKHQDPKFDPESSSTYQAV+CN+DCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF
Sbjct: 121 VECGKHQDPKFDPESSSTYQAVKCNNDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF 180
Query: 181 GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG 240
GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG
Sbjct: 181 GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG 240
Query: 241 GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGT 300
GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGT
Sbjct: 241 GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGT 300
Query: 301 VLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFP 360
VLDSGTTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFS AGSDAADLSDIFP
Sbjct: 301 VLDSGTTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSGAGSDAADLSDIFP 360
Query: 361 TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK 420
TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK
Sbjct: 361 TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK 420
Query: 421 HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIFVML 474
HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIF+ +
Sbjct: 421 HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIFLQI 479
BLAST of Cp4.1LG05g11730 vs. NCBI nr
Match:
XP_023532084.1 (aspartic proteinase nepenthesin-2-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 458/459 (99.78%), Postives = 458/459 (99.78%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSE 459
FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAP E
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPKE 459
BLAST of Cp4.1LG05g11730 vs. NCBI nr
Match:
XP_023532083.1 (aspartic proteinase nepenthesin-2-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 458/459 (99.78%), Postives = 458/459 (99.78%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSE 459
FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAP E
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPKE 459
BLAST of Cp4.1LG05g11730 vs. NCBI nr
Match:
XP_022948178.1 (aspartic proteinase nepenthesin-2-like isoform X4 [Cucurbita moschata])
HSP 1 Score: 931 bits (2406), Expect = 0.0
Identity = 454/463 (98.06%), Postives = 459/463 (99.14%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPRRHLRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPRRHLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFSS GSDAA+LSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSSGGSDAANLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRY 463
FWKTNCSELWERLHISDDNAHAPAT LDTDIAPASAPSESP +
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATLLDTDIAPASAPSESPLF 463
BLAST of Cp4.1LG05g11730 vs. ExPASy TrEMBL
Match:
A0A6J1L7E7 (aspartic proteinase nepenthesin-2-like isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111499862 PE=3 SV=1)
HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 461/479 (96.24%), Postives = 469/479 (97.91%), Query Frame = 0
Query: 1 MVRTLILL-----HFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPR 60
MVRTLILL H LHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPR
Sbjct: 1 MVRTLILLPAILIHCLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPR 60
Query: 61 RHLRDFPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC 120
RHLRDF NSNNRSNARMRLYD+LLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC
Sbjct: 61 RHLRDFTNSNNRSNARMRLYDELLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC 120
Query: 121 VECGKHQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF 180
VECGKHQDPKFDPESSSTYQAV+CN+DCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF
Sbjct: 121 VECGKHQDPKFDPESSSTYQAVKCNNDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF 180
Query: 181 GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG 240
GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG
Sbjct: 181 GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG 240
Query: 241 GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGT 300
GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGT
Sbjct: 241 GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGT 300
Query: 301 VLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFP 360
VLDSGTTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFS AGSDAADLSDIFP
Sbjct: 301 VLDSGTTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSGAGSDAADLSDIFP 360
Query: 361 TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK 420
TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK
Sbjct: 361 TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK 420
Query: 421 HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIFVML 474
HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIF+ +
Sbjct: 421 HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRYMIPDIFIFLQI 479
BLAST of Cp4.1LG05g11730 vs. ExPASy TrEMBL
Match:
A0A6J1G8N5 (aspartic proteinase nepenthesin-2-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111451832 PE=3 SV=1)
HSP 1 Score: 931 bits (2406), Expect = 0.0
Identity = 454/463 (98.06%), Postives = 459/463 (99.14%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPRRHLRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPRRHLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFSS GSDAA+LSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSSGGSDAANLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSESPRY 463
FWKTNCSELWERLHISDDNAHAPAT LDTDIAPASAPSESP +
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATLLDTDIAPASAPSESPLF 463
BLAST of Cp4.1LG05g11730 vs. ExPASy TrEMBL
Match:
A0A6J1G903 (aspartic proteinase nepenthesin-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451832 PE=3 SV=1)
HSP 1 Score: 925 bits (2390), Expect = 0.0
Identity = 451/459 (98.26%), Postives = 455/459 (99.13%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPRRHLRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPRRHLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFSS GSDAA+LSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSSGGSDAANLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSE 459
FWKTNCSELWERLHISDDNAHAPAT LDTDIAPASAP E
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATLLDTDIAPASAPKE 459
BLAST of Cp4.1LG05g11730 vs. ExPASy TrEMBL
Match:
A0A6J1G8J1 (aspartic proteinase nepenthesin-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451832 PE=3 SV=1)
HSP 1 Score: 925 bits (2390), Expect = 0.0
Identity = 451/459 (98.26%), Postives = 455/459 (99.13%), Query Frame = 0
Query: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPRRHLRD 60
MVRTLILLHFLHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPRRHLRD
Sbjct: 1 MVRTLILLHFLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPRRHLRD 60
Query: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK
Sbjct: 61 FPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGK 120
Query: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE
Sbjct: 121 HQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSE 180
Query: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG
Sbjct: 181 LIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIG 240
Query: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSG 300
GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGTVLDSG
Sbjct: 241 GGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGTVLDSG 300
Query: 301 TTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMI 360
TTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFSS GSDAA+LSDIFPTVDMI
Sbjct: 301 TTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSSGGSDAANLSDIFPTVDMI 360
Query: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG
Sbjct: 361 FDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVG 420
Query: 421 FWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSE 459
FWKTNCSELWERLHISDDNAHAPAT LDTDIAPASAP E
Sbjct: 421 FWKTNCSELWERLHISDDNAHAPATLLDTDIAPASAPKE 459
BLAST of Cp4.1LG05g11730 vs. ExPASy TrEMBL
Match:
A0A6J1L091 (aspartic proteinase nepenthesin-2-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111499862 PE=3 SV=1)
HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 448/464 (96.55%), Postives = 454/464 (97.84%), Query Frame = 0
Query: 1 MVRTLILL-----HFLHLTHLCLSTDPISSNRLLTPTHRAMVLPLYLSSPNSSRFISNPR 60
MVRTLILL H LHLTHLCLSTDPISSNRLLTP+HRAMVLPLYLSSPNSSRF+SNPR
Sbjct: 1 MVRTLILLPAILIHCLHLTHLCLSTDPISSNRLLTPSHRAMVLPLYLSSPNSSRFLSNPR 60
Query: 61 RHLRDFPNSNNRSNARMRLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC 120
RHLRDF NSNNRSNARMRLYD+LLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC
Sbjct: 61 RHLRDFTNSNNRSNARMRLYDELLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTC 120
Query: 121 VECGKHQDPKFDPESSSTYQAVECNSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF 180
VECGKHQDPKFDPESSSTYQAV+CN+DCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF
Sbjct: 121 VECGKHQDPKFDPESSSTYQAVKCNNDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISF 180
Query: 181 GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG 240
GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG
Sbjct: 181 GNQSELIPQRAVFGCENEETGDLYSQRADGIMGLGRGDLSIVDQLVDKGVINDSFSLCYG 240
Query: 241 GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFDGRYGT 300
GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKL LSPSVFDGRYGT
Sbjct: 241 GMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLVLSPSVFDGRYGT 300
Query: 301 VLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSDIFP 360
VLDSGTTYAYLPEEAFGAFKDAIM+ELHSLKKIDGPDPNFKDTCFS AGSDAADLSDIFP
Sbjct: 301 VLDSGTTYAYLPEEAFGAFKDAIMDELHSLKKIDGPDPNFKDTCFSGAGSDAADLSDIFP 360
Query: 361 TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK 420
TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK
Sbjct: 361 TVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGIFGNANDQTTLLGGIVVRNTLVMYDRK 420
Query: 421 HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPSE 459
HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAP E
Sbjct: 421 HSKVGFWKTNCSELWERLHISDDNAHAPATSLDTDIAPASAPKE 464
BLAST of Cp4.1LG05g11730 vs. TAIR 10
Match:
AT3G50050.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 609.0 bits (1569), Expect = 3.3e-174
Identity = 294/431 (68.21%), Postives = 350/431 (81.21%), Query Frame = 0
Query: 32 THRAMVLPLYLSSPN-SSRFISNPRRHLRDFPNSNNRSNARMRLYDDLLLNGYYTTKLWI 91
+ R MV PL+LS PN SSR IS P R L +S + ++RMRLYDDLL+NGYYTT+LWI
Sbjct: 41 SRRPMVFPLFLSQPNSSSRSISIPHRKLHK-SDSKSLPHSRMRLYDDLLINGYYTTRLWI 100
Query: 92 GTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPKFDPESSSTYQAVECNSDCNCDNDGL 151
GTPPQ FALIVD+GSTVTYVPCS C +CGKHQDPKF PE SSTYQ V+CN DCNCD+D
Sbjct: 101 GTPPQMFALIVDSGSTVTYVPCSDCEQCGKHQDPKFQPEMSSTYQPVKCNMDCNCDDDRE 160
Query: 152 QCIYERKYAEMSTSSGVLGEDVISFGNQSELIPQRAVFGCENEETGDLYSQRADGIMGLG 211
QC+YER+YAE S+S GVLGED+ISFGN+S+L PQRAVFGCE ETGDLYSQRADGI+GLG
Sbjct: 161 QCVYEREYAEHSSSKGVLGEDLISFGNESQLTPQRAVFGCETVETGDLYSQRADGIIGLG 220
Query: 212 RGDLSIVDQLVDKGVINDSFSLCYGGMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNV 271
+GDLS+VDQLVDKG+I++SF LCYGGMD+GGG+M+LGG PSDM+F+ SDP RSPYYN+
Sbjct: 221 QGDLSLVDQLVDKGLISNSFGLCYGGMDVGGGSMILGGFDYPSDMVFTDSDPDRSPYYNI 280
Query: 272 DLKGIHVAGKKLGLSPSVFDGRYGTVLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDG 331
DL GI VAGK+L L VFDG +G VLDSGTTYAYLP+ AF AF++A+M E+ +LK+IDG
Sbjct: 281 DLTGIRVAGKQLSLHSRVFDGEHGAVLDSGTTYAYLPDAAFAAFEEAVMREVSTLKQIDG 340
Query: 332 PDPNFKDTCFSSAGSD-AADLSDIFPTVDMIFDHEQKLSLAPENYLFPHSKIHGAYCLGI 391
PDPNFKDTCF A S+ ++LS IFP+V+M+F Q L+PENY+F HSK+HGAYCLG+
Sbjct: 341 PDPNFKDTCFQVAASNYVSELSKIFPSVEMVFKSGQSWLLSPENYMFRHSKVHGAYCLGV 400
Query: 392 FGNANDQTTLLGGIVVRNTLVMYDRKHSKVGFWKTNCSELWERLHISDDNAHAPATSLDT 451
F N D TTLLGGIVVRNTLV+YDR++SKVGFW+TNCSEL +RLHI D A PAT
Sbjct: 401 FPNGKDHTTLLGGIVVRNTLVVYDRENSKVGFWRTNCSELSDRLHI--DGAPPPATLPSN 460
Query: 452 DIAPASAPSES 461
D P+ S +
Sbjct: 461 DSNPSHNSSSN 468
BLAST of Cp4.1LG05g11730 vs. TAIR 10
Match:
AT5G43100.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 594.3 bits (1531), Expect = 8.3e-170
Identity = 290/445 (65.17%), Postives = 349/445 (78.43%), Query Frame = 0
Query: 28 LLTPTHRAMVLPL-YLSSPNSSRFISNPRRHLRDFPNSNNRSNARMRLYDDLLLNGYYTT 87
L T M+ PL Y S P R RR L + + NA M+LYDDLL NGYYTT
Sbjct: 23 LTTADESPMIFPLSYSSLPPRPRVEDFRRRRL----HQSQLPNAHMKLYDDLLSNGYYTT 82
Query: 88 KLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPKFDPESSSTYQAVECNSDCNCD 147
+LWIGTPPQ+FALIVDTGSTVTYVPCSTC +CGKHQDPKF PE S++YQA++CN DCNCD
Sbjct: 83 RLWIGTPPQEFALIVDTGSTVTYVPCSTCKQCGKHQDPKFQPELSTSYQALKCNPDCNCD 142
Query: 148 NDGLQCIYERKYAEMSTSSGVLGEDVISFGNQSELIPQRAVFGCENEETGDLYSQRADGI 207
++G C+YER+YAEMS+SSGVL ED+ISFGN+S+L PQRAVFGCENEETGDL+SQRADGI
Sbjct: 143 DEGKLCVYERRYAEMSSSSGVLSEDLISFGNESQLSPQRAVFGCENEETGDLFSQRADGI 202
Query: 208 MGLGRGDLSIVDQLVDKGVINDSFSLCYGGMDIGGGAMVLGGISPPSDMIFSYSDPVRSP 267
MGLGRG LS+VDQLVDKGVI D FSLCYGGM++GGGAMVLG ISPP M+FS+SDP RSP
Sbjct: 203 MGLGRGKLSVVDQLVDKGVIEDVFSLCYGGMEVGGGAMVLGKISPPPGMVFSHSDPFRSP 262
Query: 268 YYNVDLKGIHVAGKKLGLSPSVFDGRYGTVLDSGTTYAYLPEEAFGAFKDAIMNELHSLK 327
YYN+DLK +HVAGK L L+P VF+G++GTVLDSGTTYAY P+EAF A KDA++ E+ SLK
Sbjct: 263 YYNIDLKQMHVAGKSLKLNPKVFNGKHGTVLDSGTTYAYFPKEAFIAIKDAVIKEIPSLK 322
Query: 328 KIDGPDPNFKDTCFSSAGSDAADLSDIFPTVDMIFDHEQKLSLAPENYLFPHSKIHGAYC 387
+I GPDPN+ D CFS AG D A++ + FP + M F + QKL L+PENYLF H+K+ GAYC
Sbjct: 323 RIHGPDPNYDDVCFSGAGRDVAEIHNFFPEIAMEFGNGQKLILSPENYLFRHTKVRGAYC 382
Query: 388 LGIFGNANDQTTLLGGIVVRNTLVMYDRKHSKVGFWKTNCSELWERLHISDDNAHAPATS 447
LGIF + D TTLLGGIVVRNTLV YDR++ K+GF KTNCS++W RL + A S
Sbjct: 383 LGIFPD-RDSTTLLGGIVVRNTLVTYDRENDKLGFLKTNCSDIWRRLAAPESPAPTSPIS 442
Query: 448 LD--TDIAPASAPSESPRYMIPDIF 470
+ ++I+P+ A SESP +P +F
Sbjct: 443 QNKSSNISPSPATSESPTSHLPGVF 462
BLAST of Cp4.1LG05g11730 vs. TAIR 10
Match:
AT1G08210.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 197.6 bits (501), Expect = 2.3e-50
Identity = 145/439 (33.03%), Postives = 217/439 (49.43%), Query Frame = 0
Query: 14 THLCLSTDPISSNRLLTPTHRAMVLPL-YLSSPNSSRFISNPRRHLRDFPNSNNRSNARM 73
T C S + RL+ P H + L S R + +P + +FP
Sbjct: 22 TLACGSDAVLKLERLIPPNHELGLTELRAFDSARHGRLLQSPVGGVVNFPVDG------- 81
Query: 74 RLYDDLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPK-----FD 133
D L G Y TK+ +GTPP++F + +DTGS V +V C++C C K + + FD
Sbjct: 82 --ASDPFLVGLYYTKVKLGTPPREFNVQIDTGSDVLWVSCTSCNGCPKTSELQIQLSFFD 141
Query: 134 PESSSTYQAVECN---------SDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFG-- 193
P SS+ V C+ ++ C + L C Y KY + S +SG D +SF
Sbjct: 142 PGVSSSASLVSCSDRRCYSNFQTESGCSPNNL-CSYSFKYGDGSGTSGYYISDFMSFDTV 201
Query: 194 -NQSELIPQRA--VFGCENEETGDLYSQR--ADGIMGLGRGDLSIVDQLVDKGVINDSFS 253
+ I A VFGC N ++GDL R DGI GLG+G LS++ QL +G+ FS
Sbjct: 202 ITSTLAINSSAPFVFGCSNLQSGDLQRPRRAVDGIFGLGQGSLSVISQLAVQGLAPRVFS 261
Query: 254 LCYGGMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVFD- 313
C G GGG MVLG I P D +++ P P+YNV+L+ I V G+ L + PSVF
Sbjct: 262 HCLKGDKSGGGIMVLGQIKRP-DTVYTPLVP-SQPHYNVNLQSIAVNGQILPIDPSVFTI 321
Query: 314 -GRYGTVLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAAD 373
GT++D+GTT AYLP+EA+ F A+ N ++ + P CF D
Sbjct: 322 ATGDGTIIDTGTTLAYLPDEAYSPFIQAVAN---AVSQYGRPITYESYQCFEITAGDV-- 381
Query: 374 LSDIFPTVDMIFDHEQKLSLAPENYLFPHSKIHGA-YCLGIFGNANDQTTLLGGIVVRNT 428
D+FP V + F + L P YL S + +C+G ++ + T+LG +V+++
Sbjct: 382 --DVFPQVSLSFAGGASMVLGPRAYLQIFSSSGSSIWCIGFQRMSHRRITILGDLVLKDK 441
BLAST of Cp4.1LG05g11730 vs. TAIR 10
Match:
AT5G22850.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 197.2 bits (500), Expect = 3.0e-50
Identity = 132/377 (35.01%), Postives = 195/377 (51.72%), Query Frame = 0
Query: 77 DLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQDPK-----FDPESS 136
D + G Y TKL +GTPP+ F + VDTGS V +V C++C C + + FDP SS
Sbjct: 74 DPFVVGLYYTKLRLGTPPRDFYVQVDTGSDVLWVSCASCNGCPQTSGLQIQLNFFDPGSS 133
Query: 137 STYQAVEC----------NSDCNCDNDGLQCIYERKYAEMSTSSGVLGEDVISFGN--QS 196
T + C +SD C C Y +Y + S +SG DV+ F S
Sbjct: 134 VTASPISCSDQRCSWGIQSSDSGCSVQNNLCAYTFQYGDGSGTSGFYVSDVLQFDMIVGS 193
Query: 197 ELIPQR---AVFGCENEETGDLY-SQRA-DGIMGLGRGDLSIVDQLVDKGVINDSFSLCY 256
L+P VFGC +TGDL S RA DGI G G+ +S++ QL +G+ FS C
Sbjct: 194 SLVPNSTAPVVFGCSTSQTGDLVKSDRAVDGIFGFGQQGMSVISQLASQGIAPRVFSHCL 253
Query: 257 GGMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSVF--DGR 316
G + GGG +VLG I P +M+F+ P P+YNV+L I V G+ L ++PSVF
Sbjct: 254 KGENGGGGILVLGEIVEP-NMVFTPLVP-SQPHYNVNLLSISVNGQALPINPSVFSTSNG 313
Query: 317 YGTVLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSDAADLSD 376
GT++D+GTT AYL E A+ F +AI N + + P + + C+ S + D
Sbjct: 314 QGTIIDTGTTLAYLSEAAYVPFVEAITNAVSQSVR---PVVSKGNQCYVITTS----VGD 373
Query: 377 IFPTVDMIFDHEQKLSLAPENYLFPHSKIHG--AYCLGIFGNANDQTTLLGGIVVRNTLV 428
IFP V + F + L P++YL + + G +C+G N T+LG +V+++ +
Sbjct: 374 IFPPVSLNFAGGASMFLNPQDYLIQQNNVGGTAVWCIGFQRIQNQGITILGDLVLKDKIF 433
BLAST of Cp4.1LG05g11730 vs. TAIR 10
Match:
AT2G36670.2 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 184.9 bits (468), Expect = 1.5e-46
Identity = 130/383 (33.94%), Postives = 200/383 (52.22%), Query Frame = 0
Query: 77 DLLLNGYYTTKLWIGTPPQQFALIVDTGSTVTYVPCSTCVECGKHQD-----PKFDPESS 136
D L G Y TK+ +G+PP +F + +DTGS + +V CS+C C FD S
Sbjct: 93 DPYLVGLYFTKVKLGSPPTEFNVQIDTGSDILWVTCSSCSNCPHSSGLGIDLHFFDAPGS 152
Query: 137 STYQAVECNSDCNCD----------NDGLQCIYERKYAEMSTSSG-----------VLGE 196
T +V C SD C ++ QC Y +Y + S +SG +LGE
Sbjct: 153 LTAGSVTC-SDPICSSVFQTTAAQCSENNQCGYSFRYGDGSGTSGYYMTDTFYFDAILGE 212
Query: 197 DVISFGNQSELIPQRAVFGCENEETGDL--YSQRADGIMGLGRGDLSIVDQLVDKGVIND 256
+++ N S I VFGC ++GDL + DGI G G+G LS+V QL +G+
Sbjct: 213 SLVA--NSSAPI----VFGCSTYQSGDLTKSDKAVDGIFGFGKGKLSVVSQLSSRGITPP 272
Query: 257 SFSLCYGGMDIGGGAMVLGGISPPSDMIFSYSDPVRSPYYNVDLKGIHVAGKKLGLSPSV 316
FS C G GGG VLG I P M++S P P+YN++L I V G+ L L +V
Sbjct: 273 VFSHCLKGDGSGGGVFVLGEILVPG-MVYSPLVP-SQPHYNLNLLSIGVNGQMLPLDAAV 332
Query: 317 FD--GRYGTVLDSGTTYAYLPEEAFGAFKDAIMNELHSLKKIDGPDPNFKDTCFSSAGSD 376
F+ GT++D+GTT YL +EA+ F +AI N S+ ++ P + + C+ + S
Sbjct: 333 FEASNTRGTIVDTGTTLTYLVKEAYDLFLNAISN---SVSQLVTPIISNGEQCYLVSTS- 392
Query: 377 AADLSDIFPTVDMIFDHEQKLSLAPENYLFPHSKIHGA--YCLGIFGNANDQTTLLGGIV 428
+SD+FP+V + F + L P++YLF + GA +C+G F A ++ T+LG +V
Sbjct: 393 ---ISDMFPSVSLNFAGGASMMLRPQDYLFHYGIYDGASMWCIG-FQKAPEEQTILGDLV 452
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q4V3D2 | 5.1e-39 | 29.91 | Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1 | [more] |
Q9S9K4 | 4.8e-37 | 29.38 | Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2 | [more] |
Q9LS40 | 8.2e-37 | 29.67 | Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... | [more] |
Q766C2 | 2.6e-35 | 31.78 | Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... | [more] |
Q9LHE3 | 4.5e-35 | 30.66 | Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... | [more] |
Match Name | E-value | Identity | Description | |
KAG7035174.1 | 0.0 | 98.11 | Aspartic proteinase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_023007338.1 | 0.0 | 96.24 | aspartic proteinase nepenthesin-2-like isoform X5 [Cucurbita maxima] | [more] |
XP_023532084.1 | 0.0 | 99.78 | aspartic proteinase nepenthesin-2-like isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
XP_023532083.1 | 0.0 | 99.78 | aspartic proteinase nepenthesin-2-like isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_022948178.1 | 0.0 | 98.06 | aspartic proteinase nepenthesin-2-like isoform X4 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1L7E7 | 0.0 | 96.24 | aspartic proteinase nepenthesin-2-like isoform X5 OS=Cucurbita maxima OX=3661 GN... | [more] |
A0A6J1G8N5 | 0.0 | 98.06 | aspartic proteinase nepenthesin-2-like isoform X4 OS=Cucurbita moschata OX=3662 ... | [more] |
A0A6J1G903 | 0.0 | 98.26 | aspartic proteinase nepenthesin-2-like isoform X1 OS=Cucurbita moschata OX=3662 ... | [more] |
A0A6J1G8J1 | 0.0 | 98.26 | aspartic proteinase nepenthesin-2-like isoform X2 OS=Cucurbita moschata OX=3662 ... | [more] |
A0A6J1L091 | 0.0 | 96.55 | aspartic proteinase nepenthesin-2-like isoform X3 OS=Cucurbita maxima OX=3661 GN... | [more] |