Cp4.1LG05g10560 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g10560
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCp4.1LG05: 7063600 .. 7064829 (+)
RNA-Seq ExpressionCp4.1LG05g10560
SyntenyCp4.1LG05g10560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCAAGCAAAACAATCTCTTCATCACTGTTCCCACTCACTTCCGTTGCCCAATTTCTCTCGATCTCATGAAATCTCCAGTCAGTTTGGCCACCGGCGTCACCTACGACCGCTCCAGCATCCAGCGCTGGCTCGACTCCGGCAACAATACCTGTCCGGCTACTATGCAGCTCCTTCTCTCCAAGGATTTCGTTCCCAATTGCAATCTCAAGCGCCTTATTCAGATTTGGTCCCACACTCTCCACTTCCAATTTCCCCTCCATGATCATCATTTGGGGACGAATGCAACTCCGATTCGAAACCACTCTCACCCATCTTGCTCTGTTTCTGTTTCTCTCTCTAACTTACTCTGTTTTGCACCACCATTGGAGCAGAACGTCGAGTTTCTCCGCACGATTCACGGCCTTGTCCCCGTGCTTCTCGATGTTCTTGGAGGGGAGGGTAGTTGTGGTGATATTGAATGTTTTGAATTGGCGGTTAAGGTTTTGGATTTGGTGAGATGCGAAATTGAGGATCGAGAGGAGTTTATGAACTCGATGCTGAAAAGCAATCGCGATTGTCTGAGATCTCTGGCTCTTGTTCTGCAGCGAGGGAGTACGGAATCGCGAATTGGAACGGCGAGGATTCTGGAGTACGTCGCCATTAACGCCGAATCGAAGACCCTAATCGCCGAGAACGAAGGGATTCTATCCGAACTGATAGCTCTAATCGGAGTAGACAAAGATATGAAGCTAATCGAATCGGTGCTATCGTGCTTAATCGCAATATCAATGCCGAGACGCGTGAAAATCAGACTGGTTCAGTTAGGGGTAATAAAAGCCCTAACGAGGCTACTGTTGCAGAGGAACGCCGCCGTGGGGGTAACGGAGAAGGTTATGAGGCTGTTGGCGGCAGCGGCAGCGGTGGAGGAAGGGCGGTCGGAGATGTGCGAAAACGGAGGCGAATGCGTGGGTGGAATGGTGAGGAAAGTGATGAAGGTGTCGAGTGCGGCGACGGAGCAGGCGGTGACGGCGCTGTGGTGCATATGCTATTTGTTCCGGGAGGAGAGGGCTTTGGTGGCGGTGGCGGAGGCAAAAGGGGTGGAGAAGATACTGCTGGTGCTGCAGAGCGAGTGCCCGGCGCCGGTGAGGCAAATGGGGAAAGATCTGCTAAAGATCCTGAAGGTTAATTCCAAGTCCTTCACCCTCTCGTTTTATGATACTAACAGTTCTTACATTATGCCATTTTGA

mRNA sequence

ATGGTCAAGCAAAACAATCTCTTCATCACTGTTCCCACTCACTTCCGTTGCCCAATTTCTCTCGATCTCATGAAATCTCCAGTCAGTTTGGCCACCGGCGTCACCTACGACCGCTCCAGCATCCAGCGCTGGCTCGACTCCGGCAACAATACCTGTCCGGCTACTATGCAGCTCCTTCTCTCCAAGGATTTCGTTCCCAATTGCAATCTCAAGCGCCTTATTCAGATTTGGTCCCACACTCTCCACTTCCAATTTCCCCTCCATGATCATCATTTGGGGACGAATGCAACTCCGATTCGAAACCACTCTCACCCATCTTGCTCTGTTTCTGTTTCTCTCTCTAACTTACTCTGTTTTGCACCACCATTGGAGCAGAACGTCGAGTTTCTCCGCACGATTCACGGCCTTGTCCCCGTGCTTCTCGATGTTCTTGGAGGGGAGGGTAGTTGTGGTGATATTGAATGTTTTGAATTGGCGGTTAAGGTTTTGGATTTGGTGAGATGCGAAATTGAGGATCGAGAGGAGTTTATGAACTCGATGCTGAAAAGCAATCGCGATTGTCTGAGATCTCTGGCTCTTGTTCTGCAGCGAGGGAGTACGGAATCGCGAATTGGAACGGCGAGGATTCTGGAGTACGTCGCCATTAACGCCGAATCGAAGACCCTAATCGCCGAGAACGAAGGGATTCTATCCGAACTGATAGCTCTAATCGGAGTAGACAAAGATATGAAGCTAATCGAATCGGTGCTATCGTGCTTAATCGCAATATCAATGCCGAGACGCGTGAAAATCAGACTGGTTCAGTTAGGGGTAATAAAAGCCCTAACGAGGCTACTGTTGCAGAGGAACGCCGCCGTGGGGGTAACGGAGAAGGTTATGAGGCTGTTGGCGGCAGCGGCAGCGGTGGAGGAAGGGCGGTCGGAGATGTGCGAAAACGGAGGCGAATGCGTGGGTGGAATGGTGAGGAAAGTGATGAAGGTGTCGAGTGCGGCGACGGAGCAGGCGGTGACGGCGCTGTGGTGCATATGCTATTTGTTCCGGGAGGAGAGGGCTTTGGTGGCGGTGGCGGAGGCAAAAGGGGTGGAGAAGATACTGCTGGTGCTGCAGAGCGAGTGCCCGGCGCCGGTGAGGCAAATGGGGAAAGATCTGCTAAAGATCCTGAAGGTTAATTCCAAGTCCTTCACCCTCTCGTTTTATGATACTAACAGTTCTTACATTATGCCATTTTGA

Coding sequence (CDS)

ATGGTCAAGCAAAACAATCTCTTCATCACTGTTCCCACTCACTTCCGTTGCCCAATTTCTCTCGATCTCATGAAATCTCCAGTCAGTTTGGCCACCGGCGTCACCTACGACCGCTCCAGCATCCAGCGCTGGCTCGACTCCGGCAACAATACCTGTCCGGCTACTATGCAGCTCCTTCTCTCCAAGGATTTCGTTCCCAATTGCAATCTCAAGCGCCTTATTCAGATTTGGTCCCACACTCTCCACTTCCAATTTCCCCTCCATGATCATCATTTGGGGACGAATGCAACTCCGATTCGAAACCACTCTCACCCATCTTGCTCTGTTTCTGTTTCTCTCTCTAACTTACTCTGTTTTGCACCACCATTGGAGCAGAACGTCGAGTTTCTCCGCACGATTCACGGCCTTGTCCCCGTGCTTCTCGATGTTCTTGGAGGGGAGGGTAGTTGTGGTGATATTGAATGTTTTGAATTGGCGGTTAAGGTTTTGGATTTGGTGAGATGCGAAATTGAGGATCGAGAGGAGTTTATGAACTCGATGCTGAAAAGCAATCGCGATTGTCTGAGATCTCTGGCTCTTGTTCTGCAGCGAGGGAGTACGGAATCGCGAATTGGAACGGCGAGGATTCTGGAGTACGTCGCCATTAACGCCGAATCGAAGACCCTAATCGCCGAGAACGAAGGGATTCTATCCGAACTGATAGCTCTAATCGGAGTAGACAAAGATATGAAGCTAATCGAATCGGTGCTATCGTGCTTAATCGCAATATCAATGCCGAGACGCGTGAAAATCAGACTGGTTCAGTTAGGGGTAATAAAAGCCCTAACGAGGCTACTGTTGCAGAGGAACGCCGCCGTGGGGGTAACGGAGAAGGTTATGAGGCTGTTGGCGGCAGCGGCAGCGGTGGAGGAAGGGCGGTCGGAGATGTGCGAAAACGGAGGCGAATGCGTGGGTGGAATGGTGAGGAAAGTGATGAAGGTGTCGAGTGCGGCGACGGAGCAGGCGGTGACGGCGCTGTGGTGCATATGCTATTTGTTCCGGGAGGAGAGGGCTTTGGTGGCGGTGGCGGAGGCAAAAGGGGTGGAGAAGATACTGCTGGTGCTGCAGAGCGAGTGCCCGGCGCCGGTGAGGCAAATGGGGAAAGATCTGCTAAAGATCCTGAAGGTTAATTCCAAGTCCTTCACCCTCTCGTTTTATGATACTAACAGTTCTTACATTATGCCATTTTGA

Protein sequence

MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLSKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Homology
BLAST of Cp4.1LG05g10560 vs. ExPASy Swiss-Prot
Match: Q9FLF4 (U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.4e-92
Identity = 199/424 (46.93%), Postives = 278/424 (65.57%), Query Frame = 0

Query: 2   VKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLS 61
           +++++L ITVPT FRCPISLD+MKSPVSL TGVTYDR+SIQRWLD GNNTCPATMQ+L +
Sbjct: 1   MRKDDLCITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQN 60

Query: 62  KDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCS-VSVS-------- 121
           KDF+PN  L+RLI+IWS ++  +  +    L   A P R+    +   V +         
Sbjct: 61  KDFIPNRTLQRLIEIWSDSVRRRTCVESAEL---AAPTRDEIADAIDRVKIEKEERDDRE 120

Query: 122 -LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLG----GEGSCGDIECFELAVKVLDLVR 181
            LS ++ F    + N  FL      V +L+D++        S       + AVK+L  +R
Sbjct: 121 VLSKIVRFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIR 180

Query: 182 CEIEDREEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENE 241
            ++ DR  F N +L + RD L  +  + + G+ E +I  A +LE++A++AESK LIAE +
Sbjct: 181 SKVSDRRRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERD 240

Query: 242 GILSELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNA--A 301
           G+++EL+  I  D D+ LIES LSCLIAIS P+RVK+ L++  +I  +T+LL    +  +
Sbjct: 241 GLITELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLS 300

Query: 302 VGVTEKVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYL 361
           V VTEK ++LL   A+ +EGRSE+C   GEC+  +V+K+MKVS+AATE AVT LW + YL
Sbjct: 301 VSVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYL 360

Query: 362 FREERALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSY 410
           F+E++AL AV    GV KILL+LQS C   VR+M  DLLK+ KVNS+S  LS YDT +++
Sbjct: 361 FKEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVNSRS-CLSAYDTKTTH 420

BLAST of Cp4.1LG05g10560 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 5.5e-90
Identity = 193/415 (46.51%), Postives = 278/415 (66.99%), Query Frame = 0

Query: 2   VKQNNLFI-TVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 61
           ++ ++L+I TVP  F+CPISLD+MKSPVSL+TGVTYDR SIQRWLD GNNTCPATMQ+L 
Sbjct: 1   MRSDDLYITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGNNTCPATMQILQ 60

Query: 62  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCS----VSVSLSNL 121
           +K+FVPN  L RLI  WS +++ +    D     + TP R+  + +       + + S +
Sbjct: 61  NKEFVPNLTLHRLIDHWSDSINRR---ADSESPESDTPTRDEINAAIERFRIENDARSKI 120

Query: 122 LCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFEL--AVKVLDLVRCEIEDRE 181
           L FA   ++N EFL      V +L+D++    +  D +   +  AVK+L ++R +I DR 
Sbjct: 121 LRFARESDENREFLAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMIRRKIFDRR 180

Query: 182 EFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELI 241
              N +L +  DCL S  L+++RG+ + +I  + +LE++A++AESK +IA+ EG+++E+I
Sbjct: 181 RLSNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESKLIIAKGEGLVTEII 240

Query: 242 ALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMR 301
            LI  D D  LIE+ LS LIAI+  +RVK+ L++  ++  LT LL     +V VTEK ++
Sbjct: 241 KLISSDSDSSLIEANLSLLIAIASSKRVKLALIREKLVTKLTSLLTDPTTSVSVTEKCLK 300

Query: 302 LLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVA 361
           LL A ++ +EGRSE+C+  G CV  +V K+MKVS+AATE AVT LW +CYLF+E++A  A
Sbjct: 301 LLEAISSCKEGRSEICD--GVCVETVVNKLMKVSTAATEHAVTVLWSVCYLFKEKKAQDA 360

Query: 362 VAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 410
           V    GV KILL+LQS C   VR M  DLLK+ KVNS+S  LS Y+T +++IMPF
Sbjct: 361 VIRINGVTKILLLLQSNCSLTVRHMLTDLLKVFKVNSRS-CLSVYETKTTHIMPF 409

BLAST of Cp4.1LG05g10560 vs. ExPASy Swiss-Prot
Match: Q9LSA6 (U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.6e-84
Identity = 185/419 (44.15%), Postives = 274/419 (65.39%), Query Frame = 0

Query: 4   QNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLSKD 63
           +   +ITVP+ F+CPISLD+M+SPVSL TGVTYDR+SIQRWLD GNNTCPATMQLL +KD
Sbjct: 5   ETETYITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKD 64

Query: 64  FVPNCNLKRLIQIWSHTL----HFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLL-- 123
           FVPN  L+RLI IWS ++    +   P+ +   G    P +   +      +SL NL+  
Sbjct: 65  FVPNLTLQRLINIWSDSIGRRHNGDSPVLNPPSG-REVPTKEEVNVLLERLMSLENLMKI 124

Query: 124 -CFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEF 183
             F    + N EFL      VP+L+D++  + +   IE   +A+++LD ++    DRE  
Sbjct: 125 VRFVKDSDSNREFLSKKMEFVPMLVDIIRTKKT--KIELVIMAIRILDSIKV---DRERL 184

Query: 184 MNSMLKSN-RDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIA 243
            N ML ++  DCL ++ L +QRG+ ES+I + R+L++++ +A+SK +IAE +G+L+E++ 
Sbjct: 185 SNLMLANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMK 244

Query: 244 LIGV--DKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAA-VGVTEKV 303
            I +    D  LIE+ LS LI IS  +RV+ +L+    I  +  +LL      V VTEK 
Sbjct: 245 SISITESSDPSLIEASLSFLITISKSKRVRSKLIAAKAITKIKDILLTETLTNVAVTEKS 304

Query: 304 MRLLAAAAAVEEGRSEMC-ENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERA 363
           ++LL   ++  EGR E+C ++ G CV G+V+K++KVS+ ATE AVT LWC+CY+FRE++ 
Sbjct: 305 LKLLETLSSKREGRLEICGDDNGRCVEGVVKKLLKVSTTATEHAVTILWCLCYVFREDKT 364

Query: 364 LVAVAE-AKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 410
           +    E + GV K+L+V+QS C A VRQM KDL+K+LK NS +  L+ Y+T +++IMPF
Sbjct: 365 VEETVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLKFNSSA--LAAYETKTTHIMPF 415

BLAST of Cp4.1LG05g10560 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.1e-25
Identity = 124/431 (28.77%), Postives = 201/431 (46.64%), Query Frame = 0

Query: 6   NLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNN-TCPATMQLLLSKDF 65
           +L I +P HFRCPISL+LM+ PV++ TG TYDR+SI+ W+  GNN TCP T   L     
Sbjct: 9   DLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTL 68

Query: 66  VPNCNLKRLIQIWSHTLHFQ------------FPLHDHHLGTNATPIRNHSHPSCSVSVS 125
           +PN  L+RLIQ W                    P     L + A+ I        S + +
Sbjct: 69  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAA 128

Query: 126 LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIED 185
           L  L  FA   ++N   L   H    +L+ +L  E +  ++    LA+    LV   I +
Sbjct: 129 LRRLRGFARDSDKN-RVLIAAHNATEILIKILFSETTSSELVSESLAL----LVMLPITE 188

Query: 186 REEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTL---IAEN--- 245
             +F++  + S+   +  L  +L   S E+R+  A ++E V+   +S  L   I+ +   
Sbjct: 189 PNQFVS--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV 248

Query: 246 -EGILSELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAA 305
            EG+L  L   I   + +K+    L  L ++   R +        +      +L+ R AA
Sbjct: 249 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHI-------AITAGAPEILIDRLAA 308

Query: 306 ---VGVTEKVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCI 365
                 TE+ +  +       EG +   E+    V  +V+ +++VS  ATE A  AL  +
Sbjct: 309 DFDRCDTERALATVELLCRTPEGCAAFGEH-ALTVPLLVKTILRVSDRATEYAAGALLAL 368

Query: 366 CYLFREERALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTN 410
           C    EER     A A  V ++LL++QSEC    ++  + LLK+L+ +   +  SF +++
Sbjct: 369 C--TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYN-SFANSD 421

BLAST of Cp4.1LG05g10560 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.5e-23
Identity = 117/400 (29.25%), Postives = 180/400 (45.00%), Query Frame = 0

Query: 11  VPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLSKDFVPNCNL 70
           VP  F CPISL+ M  PV+L TG TY+ ++I RWL  G+ TCP TMQ L     +PN  L
Sbjct: 65  VPAVFICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTL 124

Query: 71  KRLIQIWSHTLHFQFPLHD-----------HHLGTNATPIRNHSHPSCSVSVSLSNLLCF 130
           ++LI  W    + +F               H L   A P R        V+ +L  L   
Sbjct: 125 RQLIAAWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVA-ALRELRSL 184

Query: 131 APPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNS 190
           A    Q+V       G V +L  +LG   S       E    ++  V  + + +   M  
Sbjct: 185 A-AAHQSVTKAIAEAGGVGLLTSLLGPFTS--HAVGSEAVAILVSGVPLDADAKAALMQP 244

Query: 191 MLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGV 250
              S       L  +L  G+ +++I   R+   + I  E K    +    LS L+ ++ +
Sbjct: 245 AKVS------LLVDMLNEGAVDTKINCVRL---IRILMEEKGFRPDTVASLSLLVGVMRL 304

Query: 251 DKDMKLIESV------LSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVM 310
            +D +  + V      L+ + A+  P R  I  V +G +  L  LL +        E  +
Sbjct: 305 VRDKRHPDGVAAGLELLNSICAVHKPARSLI--VSIGAVPQLVELLPE--LPTECVEPAL 364

Query: 311 RLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALV 370
            +L A AAV EGR  + ++    +   VR +M+VS A T +A++ LW +C +  EE A  
Sbjct: 365 DILDALAAVPEGRIAL-KDCPRTITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPA 424

Query: 371 AVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKS 394
           A+    G  K+LLV+QS C   ++Q   +LLK+  +N  S
Sbjct: 425 ALDAGLGA-KLLLVIQSGCGPELKQQASELLKLCTMNCTS 445

BLAST of Cp4.1LG05g10560 vs. NCBI nr
Match: XP_023534066.1 (U-box domain-containing protein 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 794 bits (2050), Expect = 2.26e-289
Identity = 409/409 (100.00%), Postives = 409/409 (100.00%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM
Sbjct: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD
Sbjct: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. NCBI nr
Match: KAG7035268.1 (U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781 bits (2018), Expect = 1.70e-284
Identity = 401/409 (98.04%), Postives = 406/409 (99.27%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHP CSVSVSLSNLLCF+
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPYCSVSVSLSNLLCFS 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEI+DREEFMNSM
Sbjct: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIKDREEFMNSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNRDCLRSLALVLQRG+TESRIGTARILE +AINAESKTLIAENEGIL ELIALIGVD
Sbjct: 181 LKSNRDCLRSLALVLQRGNTESRIGTARILECIAINAESKTLIAENEGILCELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCL+AISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLVAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. NCBI nr
Match: XP_022947677.1 (U-box domain-containing protein 27-like [Cucurbita moschata])

HSP 1 Score: 781 bits (2016), Expect = 3.43e-284
Identity = 403/409 (98.53%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNVEFLRTI GLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM
Sbjct: 121 PPLEQNVEFLRTIQGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNRDCLRSLALVLQRGSTESRIGTARILE VAINAESKTLIAENEGIL ELIALIGVD
Sbjct: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILECVAINAESKTLIAENEGILCELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCL+AISMPRRVKIRLVQLGVIKALTRLLLQRN AVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLVAISMPRRVKIRLVQLGVIKALTRLLLQRNVAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AV EGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVVEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. NCBI nr
Match: KAG6605313.1 (U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 778 bits (2009), Expect = 4.00e-283
Identity = 399/409 (97.56%), Postives = 405/409 (99.02%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHP CSVSVSLSNLLCF+
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPYCSVSVSLSNLLCFS 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNV+FLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEI+DREEFMNSM
Sbjct: 121 PPLEQNVQFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIKDREEFMNSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNRDCLRSLALVLQRG+TESRIGTARILE +AINAESKTLIAENEGIL ELIALIGVD
Sbjct: 181 LKSNRDCLRSLALVLQRGNTESRIGTARILECIAINAESKTLIAENEGILCELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCL+AISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLVAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEE RSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVEEARSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. NCBI nr
Match: XP_023007179.1 (U-box domain-containing protein 27-like [Cucurbita maxima])

HSP 1 Score: 776 bits (2005), Expect = 1.63e-282
Identity = 401/409 (98.04%), Postives = 403/409 (98.53%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHT HFQFPLHDHHLGTNA PIRNH+HPSCSVSVSLSNLLCFA
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTFHFQFPLHDHHLGTNAAPIRNHAHPSCSVSVSLSNLLCFA 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNVEFLRTIHGLVPVLLDVLG EGSCGDIECFELAVKVLDLVRCEIEDREEFM SM
Sbjct: 121 PPLEQNVEFLRTIHGLVPVLLDVLGEEGSCGDIECFELAVKVLDLVRCEIEDREEFMTSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNR+CLRSLALVLQRGSTESRIGTARILE VAINAESKTLIAENEGIL ELIALIGVD
Sbjct: 181 LKSNRNCLRSLALVLQRGSTESRIGTARILECVAINAESKTLIAENEGILCELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. ExPASy TrEMBL
Match: A0A6J1G743 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451471 PE=4 SV=1)

HSP 1 Score: 781 bits (2016), Expect = 1.66e-284
Identity = 403/409 (98.53%), Postives = 404/409 (98.78%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNVEFLRTI GLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM
Sbjct: 121 PPLEQNVEFLRTIQGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNRDCLRSLALVLQRGSTESRIGTARILE VAINAESKTLIAENEGIL ELIALIGVD
Sbjct: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILECVAINAESKTLIAENEGILCELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCL+AISMPRRVKIRLVQLGVIKALTRLLLQRN AVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLVAISMPRRVKIRLVQLGVIKALTRLLLQRNVAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AV EGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVVEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. ExPASy TrEMBL
Match: A0A6J1L488 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499751 PE=4 SV=1)

HSP 1 Score: 776 bits (2005), Expect = 7.89e-283
Identity = 401/409 (98.04%), Postives = 403/409 (98.53%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL
Sbjct: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           SKDFVPNCNLKRLIQIWSHT HFQFPLHDHHLGTNA PIRNH+HPSCSVSVSLSNLLCFA
Sbjct: 61  SKDFVPNCNLKRLIQIWSHTFHFQFPLHDHHLGTNAAPIRNHAHPSCSVSVSLSNLLCFA 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
           PPLEQNVEFLRTIHGLVPVLLDVLG EGSCGDIECFELAVKVLDLVRCEIEDREEFM SM
Sbjct: 121 PPLEQNVEFLRTIHGLVPVLLDVLGEEGSCGDIECFELAVKVLDLVRCEIEDREEFMTSM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKSNR+CLRSLALVLQRGSTESRIGTARILE VAINAESKTLIAENEGIL ELIALIGVD
Sbjct: 181 LKSNRNCLRSLALVLQRGSTESRIGTARILECVAINAESKTLIAENEGILCELIALIGVD 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA
Sbjct: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG
Sbjct: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF
Sbjct: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409

BLAST of Cp4.1LG05g10560 vs. ExPASy TrEMBL
Match: A0A1S3CF07 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499732 PE=4 SV=1)

HSP 1 Score: 536 bits (1382), Expect = 2.51e-188
Identity = 292/398 (73.37%), Postives = 326/398 (81.91%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVK +NLF+T+PTHF CPISLDLM+SPVSL TGVTYDRSSIQRWLDSG+NTCPATMQ+L 
Sbjct: 1   MVKPHNLFLTIPTHFICPISLDLMQSPVSLITGVTYDRSSIQRWLDSGHNTCPATMQVLP 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           +  FVPN NLKRLIQIWS +L F       H+ T    +R +     + SV LS LLCF 
Sbjct: 61  NYGFVPNSNLKRLIQIWSDSLEFD------HILTVVDGLRTNG----NASVFLSELLCFG 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
             LE+NV+FL  IHG VPVLLDVL     C D++C EL V+VLDLVRCEIEDR EFMN M
Sbjct: 121 SRLEKNVKFLGRIHGFVPVLLDVL----RCRDVDCSELVVRVLDLVRCEIEDRVEFMNLM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKS+RDCL+SL LVLQRGS+ESRIGT R+LE +AINAESK LIAENEGIL ELI +IG+D
Sbjct: 181 LKSDRDCLKSLVLVLQRGSSESRIGTVRLLECIAINAESKNLIAENEGILHELIXVIGID 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           +D KLIESVLSCLIAISMP+RVKI+LV+LGVIKALTRLL  RNA+VGVTEKV+RLLAAAA
Sbjct: 241 EDPKLIESVLSCLIAISMPKRVKIKLVRLGVIKALTRLLKHRNASVGVTEKVLRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEEGRSEM  NGGECVG MVRKVMKVSSAATEQAVTALWCICYLFREE+A VA AEAKG
Sbjct: 301 AVEEGRSEMMVNGGECVGRMVRKVMKVSSAATEQAVTALWCICYLFREEKAAVAAAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSF 398
           VEKILL++QS CPA VR M KDLLKI K  S   T  +
Sbjct: 361 VEKILLLMQSHCPATVRVMAKDLLKIFKGYSNIITFEY 384

BLAST of Cp4.1LG05g10560 vs. ExPASy TrEMBL
Match: A0A0A0LMY5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G302090 PE=4 SV=1)

HSP 1 Score: 534 bits (1376), Expect = 2.05e-187
Identity = 291/398 (73.12%), Postives = 324/398 (81.41%), Query Frame = 0

Query: 1   MVKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 60
           MVK +NLFIT+PTHF CPISLDLM+SPVSL TGVTYDRSSIQRWLDSG+NTCPATMQ+L 
Sbjct: 1   MVKPHNLFITIPTHFLCPISLDLMQSPVSLITGVTYDRSSIQRWLDSGHNTCPATMQVLT 60

Query: 61  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLLCFA 120
           + DFVPN NLKRLIQIWS +L         H+ T    +R +     + SVSLS LLCF 
Sbjct: 61  NYDFVPNSNLKRLIQIWSDSLELD------HILTVVDGLRTNG----NASVSLSELLCFG 120

Query: 121 PPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEFMNSM 180
             LE+NV+FL  I G VPVLLDVL     C D++C EL V+VLDLVRCEIEDR EFMN M
Sbjct: 121 SRLEKNVKFLGRIRGFVPVLLDVL----RCRDVDCSELVVRVLDLVRCEIEDRVEFMNLM 180

Query: 181 LKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIALIGVD 240
           LKS+RDCLRSL LVLQRGS+ESRIGT R+LE +AINAESK LIAENEGIL ELI +IG+D
Sbjct: 181 LKSDRDCLRSLVLVLQRGSSESRIGTVRLLECIAINAESKNLIAENEGILHELIEVIGID 240

Query: 241 KDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMRLLAAAA 300
           +D KLIESVLSCLI ISMP+RVKI+LV+LGVIKALTRLL  +NA+VGVTEKV+RLLAAAA
Sbjct: 241 EDPKLIESVLSCLIPISMPKRVKIKLVRLGVIKALTRLLKHQNASVGVTEKVLRLLAAAA 300

Query: 301 AVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVAVAEAKG 360
           AVEEGR EM ENGGECVG MVRKVMKVSSAATEQAVTALWCICYL+REERA VA AEAKG
Sbjct: 301 AVEEGRWEMMENGGECVGRMVRKVMKVSSAATEQAVTALWCICYLYREERAAVAAAEAKG 360

Query: 361 VEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSF 398
           VEKILL++QS CPA VR M KDLLK  K  S   T  +
Sbjct: 361 VEKILLLMQSHCPATVRAMAKDLLKTFKGYSNIITFEY 384

BLAST of Cp4.1LG05g10560 vs. ExPASy TrEMBL
Match: A0A5N6QGH5 (RING-type E3 ubiquitin transferase OS=Carpinus fangiana OX=176857 GN=FH972_002836 PE=4 SV=1)

HSP 1 Score: 404 bits (1039), Expect = 6.68e-136
Identity = 229/419 (54.65%), Postives = 296/419 (70.64%), Query Frame = 0

Query: 4   QNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLSKD 63
           +++L+ITVP+ F+CPISLD+MKSPVSL TGVTYDRSSIQRWLDSGNNTCPATMQ+L SK+
Sbjct: 3   RDDLYITVPSFFKCPISLDVMKSPVSLCTGVTYDRSSIQRWLDSGNNTCPATMQVLQSKE 62

Query: 64  FVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCSV-----------SVS 123
           F+PN  L+RLIQIWS +        +H + +  +P   HS PS                S
Sbjct: 63  FIPNRTLQRLIQIWSDSSR------NHRVDSADSP--PHSAPSPEQLQDLIKDLDRNGES 122

Query: 124 LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLG--GEGSCGDIECFELAVKVLDLVRCEI 183
            S ++CFA   E+N +FL  + G +  L + LG  G+G C   +  E  V VLDLV  ++
Sbjct: 123 FSKIICFARESEENRKFLARMDGFIVTLAEFLGNAGDGKCWSFDFLEKVVLVLDLVLGKV 182

Query: 184 EDREEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGIL 243
            D E+  NSMLK++R+CL SL LVLQ+GSTESRI +AR+L  +A +AESK +IA+ EG+L
Sbjct: 183 VDDEQVTNSMLKTDRNCLASLLLVLQQGSTESRIASARVLGAIANDAESKLMIADKEGVL 242

Query: 244 SELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTE 303
           SEL+ LI  +KD +LIE+ LS LI ISMPRRVK RLV LG IK+L +L+   N +V VTE
Sbjct: 243 SELLKLIAPEKDPELIEAGLSSLIRISMPRRVKARLVHLGTIKSLAKLISASNPSVSVTE 302

Query: 304 KVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREER 363
           K ++LL   ++ +EGRSEMCE+   CV  +V KV KVSSAATE AVT LW +CYLFR++R
Sbjct: 303 KALKLLETVSSCKEGRSEMCEDVA-CVAAIVHKVFKVSSAATEHAVTILWSLCYLFRDQR 362

Query: 364 ALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 409
           A  AVA+A G+ KILL++QS C   VRQM  DLLKI ++NSKS  L+ YDT +++IMPF
Sbjct: 363 AQDAVAQANGLTKILLLMQSNCSPSVRQMSADLLKIFRINSKS-CLASYDTKTTHIMPF 411

BLAST of Cp4.1LG05g10560 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-93
Identity = 199/424 (46.93%), Postives = 278/424 (65.57%), Query Frame = 0

Query: 2   VKQNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLS 61
           +++++L ITVPT FRCPISLD+MKSPVSL TGVTYDR+SIQRWLD GNNTCPATMQ+L +
Sbjct: 1   MRKDDLCITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQN 60

Query: 62  KDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCS-VSVS-------- 121
           KDF+PN  L+RLI+IWS ++  +  +    L   A P R+    +   V +         
Sbjct: 61  KDFIPNRTLQRLIEIWSDSVRRRTCVESAEL---AAPTRDEIADAIDRVKIEKEERDDRE 120

Query: 122 -LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLG----GEGSCGDIECFELAVKVLDLVR 181
            LS ++ F    + N  FL      V +L+D++        S       + AVK+L  +R
Sbjct: 121 VLSKIVRFGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIR 180

Query: 182 CEIEDREEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENE 241
            ++ DR  F N +L + RD L  +  + + G+ E +I  A +LE++A++AESK LIAE +
Sbjct: 181 SKVSDRRRFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERD 240

Query: 242 GILSELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNA--A 301
           G+++EL+  I  D D+ LIES LSCLIAIS P+RVK+ L++  +I  +T+LL    +  +
Sbjct: 241 GLITELMKSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLS 300

Query: 302 VGVTEKVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYL 361
           V VTEK ++LL   A+ +EGRSE+C   GEC+  +V+K+MKVS+AATE AVT LW + YL
Sbjct: 301 VSVTEKCLKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYL 360

Query: 362 FREERALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSY 410
           F+E++AL AV    GV KILL+LQS C   VR+M  DLLK+ KVNS+S  LS YDT +++
Sbjct: 361 FKEDKALEAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVNSRS-CLSAYDTKTTH 420

BLAST of Cp4.1LG05g10560 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.9e-91
Identity = 193/415 (46.51%), Postives = 278/415 (66.99%), Query Frame = 0

Query: 2   VKQNNLFI-TVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLL 61
           ++ ++L+I TVP  F+CPISLD+MKSPVSL+TGVTYDR SIQRWLD GNNTCPATMQ+L 
Sbjct: 1   MRSDDLYITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGNNTCPATMQILQ 60

Query: 62  SKDFVPNCNLKRLIQIWSHTLHFQFPLHDHHLGTNATPIRNHSHPSCS----VSVSLSNL 121
           +K+FVPN  L RLI  WS +++ +    D     + TP R+  + +       + + S +
Sbjct: 61  NKEFVPNLTLHRLIDHWSDSINRR---ADSESPESDTPTRDEINAAIERFRIENDARSKI 120

Query: 122 LCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFEL--AVKVLDLVRCEIEDRE 181
           L FA   ++N EFL      V +L+D++    +  D +   +  AVK+L ++R +I DR 
Sbjct: 121 LRFARESDENREFLAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMIRRKIFDRR 180

Query: 182 EFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELI 241
              N +L +  DCL S  L+++RG+ + +I  + +LE++A++AESK +IA+ EG+++E+I
Sbjct: 181 RLSNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESKLIIAKGEGLVTEII 240

Query: 242 ALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAAVGVTEKVMR 301
            LI  D D  LIE+ LS LIAI+  +RVK+ L++  ++  LT LL     +V VTEK ++
Sbjct: 241 KLISSDSDSSLIEANLSLLIAIASSKRVKLALIREKLVTKLTSLLTDPTTSVSVTEKCLK 300

Query: 302 LLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERALVA 361
           LL A ++ +EGRSE+C+  G CV  +V K+MKVS+AATE AVT LW +CYLF+E++A  A
Sbjct: 301 LLEAISSCKEGRSEICD--GVCVETVVNKLMKVSTAATEHAVTVLWSVCYLFKEKKAQDA 360

Query: 362 VAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 410
           V    GV KILL+LQS C   VR M  DLLK+ KVNS+S  LS Y+T +++IMPF
Sbjct: 361 VIRINGVTKILLLLQSNCSLTVRHMLTDLLKVFKVNSRS-CLSVYETKTTHIMPF 409

BLAST of Cp4.1LG05g10560 vs. TAIR 10
Match: AT3G18710.1 (plant U-box 29 )

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-85
Identity = 185/419 (44.15%), Postives = 274/419 (65.39%), Query Frame = 0

Query: 4   QNNLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNNTCPATMQLLLSKD 63
           +   +ITVP+ F+CPISLD+M+SPVSL TGVTYDR+SIQRWLD GNNTCPATMQLL +KD
Sbjct: 5   ETETYITVPSFFKCPISLDVMRSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQLLKTKD 64

Query: 64  FVPNCNLKRLIQIWSHTL----HFQFPLHDHHLGTNATPIRNHSHPSCSVSVSLSNLL-- 123
           FVPN  L+RLI IWS ++    +   P+ +   G    P +   +      +SL NL+  
Sbjct: 65  FVPNLTLQRLINIWSDSIGRRHNGDSPVLNPPSG-REVPTKEEVNVLLERLMSLENLMKI 124

Query: 124 -CFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIEDREEF 183
             F    + N EFL      VP+L+D++  + +   IE   +A+++LD ++    DRE  
Sbjct: 125 VRFVKDSDSNREFLSKKMEFVPMLVDIIRTKKT--KIELVIMAIRILDSIKV---DRERL 184

Query: 184 MNSMLKSN-RDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTLIAENEGILSELIA 243
            N ML ++  DCL ++ L +QRG+ ES+I + R+L++++ +A+SK +IAE +G+L+E++ 
Sbjct: 185 SNLMLANDGGDCLTAILLAIQRGNLESKIESVRVLDWISFDAKSKLMIAERDGVLTEMMK 244

Query: 244 LIGV--DKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAA-VGVTEKV 303
            I +    D  LIE+ LS LI IS  +RV+ +L+    I  +  +LL      V VTEK 
Sbjct: 245 SISITESSDPSLIEASLSFLITISKSKRVRSKLIAAKAITKIKDILLTETLTNVAVTEKS 304

Query: 304 MRLLAAAAAVEEGRSEMC-ENGGECVGGMVRKVMKVSSAATEQAVTALWCICYLFREERA 363
           ++LL   ++  EGR E+C ++ G CV G+V+K++KVS+ ATE AVT LWC+CY+FRE++ 
Sbjct: 305 LKLLETLSSKREGRLEICGDDNGRCVEGVVKKLLKVSTTATEHAVTILWCLCYVFREDKT 364

Query: 364 LVAVAE-AKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTNSSYIMPF 410
           +    E + GV K+L+V+QS C A VRQM KDL+K+LK NS +  L+ Y+T +++IMPF
Sbjct: 365 VEETVERSNGVTKLLVVIQSNCSAMVRQMAKDLIKVLKFNSSA--LAAYETKTTHIMPF 415

BLAST of Cp4.1LG05g10560 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 118.2 bits (295), Expect = 1.5e-26
Identity = 124/431 (28.77%), Postives = 201/431 (46.64%), Query Frame = 0

Query: 6   NLFITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSGNN-TCPATMQLLLSKDF 65
           +L I +P HFRCPISL+LM+ PV++ TG TYDR+SI+ W+  GNN TCP T   L     
Sbjct: 9   DLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTL 68

Query: 66  VPNCNLKRLIQIWSHTLHFQ------------FPLHDHHLGTNATPIRNHSHPSCSVSVS 125
           +PN  L+RLIQ W                    P     L + A+ I        S + +
Sbjct: 69  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAA 128

Query: 126 LSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGSCGDIECFELAVKVLDLVRCEIED 185
           L  L  FA   ++N   L   H    +L+ +L  E +  ++    LA+    LV   I +
Sbjct: 129 LRRLRGFARDSDKN-RVLIAAHNATEILIKILFSETTSSELVSESLAL----LVMLPITE 188

Query: 186 REEFMNSMLKSNRDCLRSLALVLQRGSTESRIGTARILEYVAINAESKTL---IAEN--- 245
             +F++  + S+   +  L  +L   S E+R+  A ++E V+   +S  L   I+ +   
Sbjct: 189 PNQFVS--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV 248

Query: 246 -EGILSELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQLGVIKALTRLLLQRNAA 305
            EG+L  L   I   + +K+    L  L ++   R +        +      +L+ R AA
Sbjct: 249 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHI-------AITAGAPEILIDRLAA 308

Query: 306 ---VGVTEKVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKVSSAATEQAVTALWCI 365
                 TE+ +  +       EG +   E+    V  +V+ +++VS  ATE A  AL  +
Sbjct: 309 DFDRCDTERALATVELLCRTPEGCAAFGEH-ALTVPLLVKTILRVSDRATEYAAGALLAL 368

Query: 366 CYLFREERALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKILKVNSKSFTLSFYDTN 410
           C    EER     A A  V ++LL++QSEC    ++  + LLK+L+ +   +  SF +++
Sbjct: 369 C--TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYN-SFANSD 421

BLAST of Cp4.1LG05g10560 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 110.5 bits (275), Expect = 3.1e-24
Identity = 113/419 (26.97%), Postives = 188/419 (44.87%), Query Frame = 0

Query: 9   ITVPTHFRCPISLDLMKSPVSLATGVTYDRSSIQRWLDSG-NNTCPATMQLLLSKDFVPN 68
           I +P+ F CPISLD+MK PV ++TG+TYDR SI++WL SG  N+CP T Q++   D  PN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 69  CNLKRLIQIWSHTLHFQFPLHDHHLGTNATPI-----------RNHSH---PSC------ 128
             L+RLIQ W  TL+  + +    + T   PI            + SH     C      
Sbjct: 65  HTLRRLIQSWC-TLNASYGI--ERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 124

Query: 129 SVSVSLSNLLCFAPPLEQNVEFLRTIHGLVPVLLDVLGGEGS------CGDI-------E 188
            VS + +N  C      +  E    +  +V   +D      S        D+        
Sbjct: 125 IVSENTTNKRCL-----EAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLEN 184

Query: 189 CFELAVKVLD---LVRCEIEDREEFMNSML--KSNRDCLRSLALVLQRGSTESRIGTARI 248
            F+ +  ++D    V   ++  E  + S+L  K   + +++L  ++QRG  ESR   A +
Sbjct: 185 RFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALL 244

Query: 249 LEYVAINAESKTLIAENEGILSELIALIGVDKDMKLIESVLSCLIAISMPRRVKIRLVQL 308
           L+ +   A+   +I     +  E+I ++      K   S +  L+      R + + V+ 
Sbjct: 245 LKKLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEG 304

Query: 309 GVIKALTRLLLQR--NAAVGVTEKVMRLLAAAAAVEEGRSEMCENGGECVGGMVRKVMKV 368
           G I  +  LL+    ++    +E  M +L       EGR+E   N G  +  + +K+++V
Sbjct: 305 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFL-NHGAAIAVVSKKILRV 364

Query: 369 SSAATEQAVTALWCICYLFREERALVAVAEAKGVEKILLVLQSECPAPVRQMGKDLLKI 387
           S   +E+AV  L  +         L  + +   V K+ LVLQ  C    ++  K+LLK+
Sbjct: 365 SQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKL 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLF43.4e-9246.93U-box domain-containing protein 27 OS=Arabidopsis thaliana OX=3702 GN=PUB27 PE=2... [more]
Q9LXE35.5e-9046.51U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1... [more]
Q9LSA62.6e-8444.15U-box domain-containing protein 29 OS=Arabidopsis thaliana OX=3702 GN=PUB29 PE=1... [more]
Q9LT792.1e-2528.77U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q10PI91.5e-2329.25U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=T... [more]
Match NameE-valueIdentityDescription
XP_023534066.12.26e-289100.00U-box domain-containing protein 27-like [Cucurbita pepo subsp. pepo][more]
KAG7035268.11.70e-28498.04U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022947677.13.43e-28498.53U-box domain-containing protein 27-like [Cucurbita moschata][more]
KAG6605313.14.00e-28397.56U-box domain-containing protein 27, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023007179.11.63e-28298.04U-box domain-containing protein 27-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G7431.66e-28498.53RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451471... [more]
A0A6J1L4887.89e-28398.04RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499751 P... [more]
A0A1S3CF072.51e-18873.37RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499732 PE=4 ... [more]
A0A0A0LMY52.05e-18773.12RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G302090 PE... [more]
A0A5N6QGH56.68e-13654.65RING-type E3 ubiquitin transferase OS=Carpinus fangiana OX=176857 GN=FH972_00283... [more]
Match NameE-valueIdentityDescription
AT5G64660.12.4e-9346.93CYS, MET, PRO, and GLY protein 2 [more]
AT5G09800.13.9e-9146.51ARM repeat superfamily protein [more]
AT3G18710.11.9e-8544.15plant U-box 29 [more]
AT3G19380.11.5e-2628.77plant U-box 25 [more]
AT3G52450.13.1e-2426.97plant U-box 22 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 14..77
e-value: 2.9E-24
score: 96.6
IPR003613U box domainPFAMPF04564U-boxcoord: 11..78
e-value: 2.1E-18
score: 66.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 10..84
score: 32.55394
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 10..84
e-value: 4.1E-24
score: 86.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 107..391
e-value: 7.2E-15
score: 56.4
NoneNo IPR availablePANTHERPTHR22849:SF20U-BOX DOMAIN-CONTAINING PROTEIN 27-RELATEDcoord: 5..409
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 5..409
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 15..57
e-value: 8.79413E-25
score: 93.8374
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 7..79
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 163..387

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g10560.1Cp4.1LG05g10560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity