Cp4.1LG05g09380 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG05g09380 vs. ExPASy Swiss-Prot
Match: Q01587 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 1.8e-219
Identity = 375/411 (91.24%), Postives = 390/411 (94.89%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MA TV+TIGAVNRT+LNNSNYG LVPNSAFLG RLK  SSRF TTS MV GNFKIVAE D
Sbjct: 1   MAATVSTIGAVNRTTLNNSNYGGLVPNSAFLGSRLK-VSSRF-TTSKMVTGNFKIVAEQD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDI RGKGLADPLFQAPMGTGTHNA++SSYEYISAGLR 
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDITRGKGLADPLFQAPMGTGTHNAVLSSYEYISAGLRD 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           Y+ DNNVDGFYIAPAFMDKL VHIVKNF++LPNIKVPLILG+WGGKGQGKSFQCELVFAK
Sbjct: 121 YSYDNNVDGFYIAPAFMDKLTVHIVKNFLTLPNIKVPLILGVWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCL INDLDAGAGR GGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRLGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPG+YNKEENPRVPIIVTGNDFSTLYAPLIRDGRM+
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMD 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPT ED IGICTGIFRTDGVP EDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTREDRIGICTGIFRTDGVPFEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKL 412
            GVGVERIG++LVNSKE PPTFDQPKMT+EKLLEYGNML+ EQENVKRVKL
Sbjct: 361 VGVGVERIGRNLVNSKESPPTFDQPKMTIEKLLEYGNMLVMEQENVKRVKL 409

BLAST of Cp4.1LG05g09380 vs. ExPASy Swiss-Prot
Match: Q40281 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica OX=3750 GN=RCA PE=2 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 9.3e-205
Identity = 353/438 (80.59%), Postives = 385/438 (87.90%), Query Frame = 0

Query: 1   MATTVATIGAVNRT--SLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAE 60
           MAT V+TIG+VNR   +LN S+    VP+S FLG  LK  +SRF T S +  G+ +IVA 
Sbjct: 1   MATAVSTIGSVNRAPPNLNGSSSSASVPSSTFLGSSLKKVNSRF-TNSKVSSGSLRIVAS 60

Query: 61  YDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGL 120
            DE+KQT+KD+W+GLAFDTSDDQQDI RGKG  D LFQAP G+GTH AIMSSYEYIS GL
Sbjct: 61  VDEDKQTDKDRWKGLAFDTSDDQQDITRGKGKVDSLFQAPQGSGTHFAIMSSYEYISTGL 120

Query: 121 RQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVF 180
           RQYN DNN+DG+YIAPAFMDKLVVHI KNFM+LPN+KVPLILGIWGGKGQGKSFQCELVF
Sbjct: 121 RQYNFDNNMDGYYIAPAFMDKLVVHITKNFMTLPNMKVPLILGIWGGKGQGKSFQCELVF 180

Query: 181 AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGG 240
           AKM I+PIMMSAGELESGNAGEPAKLIRQRYREAAD+I+KGKMC L INDLDAGAGR GG
Sbjct: 181 AKMRISPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCALFINDLDAGAGRLGG 240

Query: 241 TTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGR 300
           TTQYTVNNQMVNATLMNIADNPTNVQLPG+YNKEENPRVPIIVTGNDFSTLYAPLIRDGR
Sbjct: 241 TTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGR 300

Query: 301 MEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRK 360
           MEKFYWAPT ED IG+C GIFR+D V  EDIVKLVDTFPGQSIDFFGALRARVYDDEVRK
Sbjct: 301 MEKFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVYDDEVRK 360

Query: 361 WAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKE 420
           W  GVGV+ IGK LVNSKEGPPTF+QPKMT+EKLLEYGNML++EQENVKRV+LA+KYL E
Sbjct: 361 WITGVGVDSIGKKLVNSKEGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLADKYLSE 420

Query: 421 AALGDANEDVLQFQTSLG 437
           AALGDAN D +   T  G
Sbjct: 421 AALGDANSDAMNTGTFYG 437

BLAST of Cp4.1LG05g09380 vs. ExPASy Swiss-Prot
Match: Q7X999 (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA2 PE=2 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 6.7e-203
Identity = 346/434 (79.72%), Postives = 386/434 (88.94%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYG---DLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVA 60
           MA   +T+GAVNR  L+ +  G    LVP++AF G  LK  +++F   S+   GNFKIVA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKKSAAKFPKASS---GNFKIVA 60

Query: 61  -EYDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISA 120
            E  E++QT+KDKW+GLA+D SDDQQDI RGKG+ D LFQAPM +GTH A+MSSY+YIS 
Sbjct: 61  QEISEDQQTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYDYISQ 120

Query: 121 GLRQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCEL 180
           GLRQYNLDNN+DGFYIAPAFMDKLVVHI KNF+SLPNIK+PLILGIWGGKGQGKSFQCEL
Sbjct: 121 GLRQYNLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKSFQCEL 180

Query: 181 VFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRF 240
           VFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCL INDLDAGAGR 
Sbjct: 181 VFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRM 240

Query: 241 GGTTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRD 300
           GGTTQYTVNNQMVNATLMNIADNPTNVQLPG+YNKEENPRVPIIVTGNDFSTLYAPLIRD
Sbjct: 241 GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRD 300

Query: 301 GRMEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEV 360
           GRMEKFYWAPT ED IG+C GIFRTD VP EDIVK+VD FPGQSIDFFGALRARVYDDEV
Sbjct: 301 GRMEKFYWAPTREDRIGVCKGIFRTDNVPEEDIVKVVDQFPGQSIDFFGALRARVYDDEV 360

Query: 361 RKWAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYL 420
           RKW + VGV+ IGK LVNSKEGPPTF+QPKMT++KLL+YGNML++EQENVKRV+LA+KY+
Sbjct: 361 RKWVSEVGVDTIGKKLVNSKEGPPTFEQPKMTIDKLLQYGNMLVEEQENVKRVQLADKYM 420

Query: 421 KEAALGDANEDVLQ 431
            EAALGDAN+D ++
Sbjct: 421 SEAALGDANQDAIK 431

BLAST of Cp4.1LG05g09380 vs. ExPASy Swiss-Prot
Match: Q7X9A0 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA1 PE=1 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 1.8e-200
Identity = 345/440 (78.41%), Postives = 384/440 (87.27%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYG---DLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVA 60
           MA   +T+GAVNR  L+ +  G    LVP++AF G  LK  +++F   S+   GNFKIVA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKKSAAKFPKASS---GNFKIVA 60

Query: 61  -EYDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISA 120
            E  E++QT+KDKW+GLA+D SDDQQDI RGKG+ D LFQAPM +GTH A+MSSY+YIS 
Sbjct: 61  QEISEDQQTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYDYISQ 120

Query: 121 GLRQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCEL 180
           GLRQYNLDNN+DGFYIAPAFMDKLVVHI KNF+SLPNIK+PLILGIWGGKGQGKSFQCEL
Sbjct: 121 GLRQYNLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKSFQCEL 180

Query: 181 VFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRF 240
           VFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCL INDLDAGAGR 
Sbjct: 181 VFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRM 240

Query: 241 GGTTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRD 300
           GGTTQYTVNNQMVNATLMNIADNPTNVQLPG+YNKEENPRVPIIVTGNDFSTLYAPLIRD
Sbjct: 241 GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRD 300

Query: 301 GRMEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEV 360
           GRMEKFYWAPT ED IG+C GIFRTD V  +DIVKLVDTFPGQSIDFFGALRARVY DEV
Sbjct: 301 GRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARVYHDEV 360

Query: 361 RKWAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYL 420
           RKW + VGV+ IGK LVNSKEGPP+F+QPKMT++KLL YG ML++EQENVKRV+LA+KY+
Sbjct: 361 RKWVSEVGVDTIGKKLVNSKEGPPSFEQPKMTIDKLLGYGGMLVQEQENVKRVQLADKYM 420

Query: 421 KEAALGDANEDVLQFQTSLG 437
            EAALGDAN D ++  T  G
Sbjct: 421 SEAALGDANNDAIKRGTFYG 437

BLAST of Cp4.1LG05g09380 vs. ExPASy Swiss-Prot
Match: Q40460 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.3e-198
Identity = 336/434 (77.42%), Postives = 379/434 (87.33%), Query Frame = 0

Query: 1   MATTVATIGAVNRT--SLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAE 60
           MAT+V+TIGAVN+T  SLNNS  G  VP++AF G+ LK    +  ++  +   + +IVAE
Sbjct: 1   MATSVSTIGAVNKTPLSLNNSVAGTSVPSTAFFGKTLKKVYGKGVSSPKVTNKSLRIVAE 60

Query: 61  ---YDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYIS 120
               D +KQT+ D+W+GL  D SDDQQDI RGKG+ D LFQAP GTGTH+A++ SYEY+S
Sbjct: 61  QIDVDPKKQTDSDRWKGLVQDFSDDQQDITRGKGMVDSLFQAPTGTGTHHAVLQSYEYVS 120

Query: 121 AGLRQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCE 180
            GLRQYNLDN +DGFYIAPAFMDKLVVHI KNF+ LPNIKVPLILGIWGGKGQGKSFQCE
Sbjct: 121 QGLRQYNLDNKLDGFYIAPAFMDKLVVHITKNFLKLPNIKVPLILGIWGGKGQGKSFQCE 180

Query: 181 LVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGR 240
           LVF KMGINPIMMSAGELESGNAGEPAKLIRQRYREAA++I+KG MCCL INDLDAGAGR
Sbjct: 181 LVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIRKGNMCCLFINDLDAGAGR 240

Query: 241 FGGTTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIR 300
            GGTTQYTVNNQMVNATLMNIADNPTNVQLPG+YNK+EN RVPIIVTGNDFSTLYAPLIR
Sbjct: 241 MGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQENARVPIIVTGNDFSTLYAPLIR 300

Query: 301 DGRMEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDE 360
           DGRMEKFYWAPT ED IG+CTGIFRTD VP ED+VK+VD FPGQSIDFFGALRARVYDDE
Sbjct: 301 DGRMEKFYWAPTREDRIGVCTGIFRTDNVPAEDVVKIVDNFPGQSIDFFGALRARVYDDE 360

Query: 361 VRKWAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKY 420
           VRKW +G G+E+IG  L+NS +GPPTF+QPKMT+EKLLEYGNML++EQENVKRV+LA+KY
Sbjct: 361 VRKWVSGTGIEKIGDKLLNSFDGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLADKY 420

Query: 421 LKEAALGDANEDVL 430
           LKEAALGDAN D +
Sbjct: 421 LKEAALGDANADAI 434

BLAST of Cp4.1LG05g09380 vs. NCBI nr
Match: XP_023532228.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532229.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532230.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023532231.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532232.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD
Sbjct: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSL 435
           LGDANEDVLQFQTSL
Sbjct: 421 LGDANEDVLQFQTSL 435

BLAST of Cp4.1LG05g09380 vs. NCBI nr
Match: XP_022947286.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita moschata] >XP_022947287.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita moschata] >XP_022947288.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita moschata] >XP_022947289.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita moschata] >XP_022947290.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 861 bits (2225), Expect = 1.16e-314
Identity = 432/435 (99.31%), Postives = 433/435 (99.54%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTVATIGAVNRTSLNNSNYG LVPNSAFLGRRLKNFSSRFTT SNMVIGNFKIVAEYD
Sbjct: 1   MATTVATIGAVNRTSLNNSNYGGLVPNSAFLGRRLKNFSSRFTT-SNMVIGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSL 435
           LGDANEDVLQFQTSL
Sbjct: 421 LGDANEDVLQFQTSL 434

BLAST of Cp4.1LG05g09380 vs. NCBI nr
Match: KAG6605441.1 (hypothetical protein SDJN03_02758, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 856 bits (2211), Expect = 5.84e-313
Identity = 426/437 (97.48%), Postives = 429/437 (98.17%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTV TIGA NRTSLNNSNYG LVPNS F G RLKNFSSRFTTTSNMV GNFKIVAEYD
Sbjct: 1   MATTVPTIGAFNRTSLNNSNYGGLVPNSTFFGSRLKNFSSRFTTTSNMVTGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKW+GLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWQGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTH+DCIGICTGIFRTD VPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHDDCIGICTGIFRTDSVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSLGK 437
           LGDANEDVLQFQTSLGK
Sbjct: 421 LGDANEDVLQFQTSLGK 437

BLAST of Cp4.1LG05g09380 vs. NCBI nr
Match: XP_023007005.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita maxima] >XP_023007006.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita maxima] >XP_023007007.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita maxima] >XP_023007008.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 855 bits (2208), Expect = 4.49e-312
Identity = 427/435 (98.16%), Postives = 429/435 (98.62%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTVATIGAVNRT LNNSNYG LVPNSAFLGRR+KNFSSRFTTTSNMVIGNFKIVAEYD
Sbjct: 1   MATTVATIGAVNRTLLNNSNYGGLVPNSAFLGRRMKNFSSRFTTTSNMVIGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNF+SLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFISLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTHEDCIGICTGIFRTDGVP EDIVKLV TFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHEDCIGICTGIFRTDGVPKEDIVKLVGTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENV RVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVMRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSL 435
           LGDANEDV QFQTSL
Sbjct: 421 LGDANEDVFQFQTSL 435

BLAST of Cp4.1LG05g09380 vs. NCBI nr
Match: KAG7035389.1 (hypothetical protein SDJN02_02185 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850 bits (2197), Expect = 2.13e-310
Identity = 423/435 (97.24%), Postives = 427/435 (98.16%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTV TIGA NRTSLNNSNYG LVPNS F G RLKNFSSRFTTTSNMV GNFKIVAEYD
Sbjct: 1   MATTVPTIGAFNRTSLNNSNYGGLVPNSTFFGSRLKNFSSRFTTTSNMVTGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKW+GLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWQGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTH+DCIGICTGIFRTD VPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHDDCIGICTGIFRTDSVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSL 435
           LGDANEDVL+FQTSL
Sbjct: 421 LGDANEDVLEFQTSL 435

BLAST of Cp4.1LG05g09380 vs. ExPASy TrEMBL
Match: A0A6J1G671 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111451195 PE=4 SV=1)

HSP 1 Score: 861 bits (2225), Expect = 5.63e-315
Identity = 432/435 (99.31%), Postives = 433/435 (99.54%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTVATIGAVNRTSLNNSNYG LVPNSAFLGRRLKNFSSRFTT SNMVIGNFKIVAEYD
Sbjct: 1   MATTVATIGAVNRTSLNNSNYGGLVPNSAFLGRRLKNFSSRFTT-SNMVIGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSL 435
           LGDANEDVLQFQTSL
Sbjct: 421 LGDANEDVLQFQTSL 434

BLAST of Cp4.1LG05g09380 vs. ExPASy TrEMBL
Match: A0A6J1KXC6 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499630 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 2.17e-312
Identity = 427/435 (98.16%), Postives = 429/435 (98.62%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MATTVATIGAVNRT LNNSNYG LVPNSAFLGRR+KNFSSRFTTTSNMVIGNFKIVAEYD
Sbjct: 1   MATTVATIGAVNRTLLNNSNYGGLVPNSAFLGRRMKNFSSRFTTTSNMVIGNFKIVAEYD 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YNLDNNVDGFYIAPAFMDKLVVHIVKNF+SLPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFISLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPTHEDCIGICTGIFRTDGVP EDIVKLV TFPGQSIDFFGALRARVYDDEVRKWA
Sbjct: 301 KFYWAPTHEDCIGICTGIFRTDGVPKEDIVKLVGTFPGQSIDFFGALRARVYDDEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENV RVKLAEKYLKEAA
Sbjct: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVMRVKLAEKYLKEAA 420

Query: 421 LGDANEDVLQFQTSL 435
           LGDANEDV QFQTSL
Sbjct: 421 LGDANEDVFQFQTSL 435

BLAST of Cp4.1LG05g09380 vs. ExPASy TrEMBL
Match: A0A6J1JCB9 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X10 OS=Cucurbita maxima OX=3661 GN=LOC111484446 PE=4 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 8.14e-288
Identity = 396/433 (91.45%), Postives = 416/433 (96.07%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MA TV+T+GAVNRT+LNNSNYG LVPNSAFLG RLK  SS+ +TTS MVIGNFKIVAEY+
Sbjct: 1   MAATVSTVGAVNRTTLNNSNYGGLVPNSAFLGSRLK-VSSK-STTSKMVIGNFKIVAEYN 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDI RGKGLADPLFQAPMGTGTHNA++SSYEY+SAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDITRGKGLADPLFQAPMGTGTHNAVLSSYEYVSAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YN DNN+DGFYIAPAFMDKL+VHIVKNFM+LPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNFDNNMDGFYIAPAFMDKLMVHIVKNFMNLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CL INDLDAGAGRFGG T
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAGAGRFGGAT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM+
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMD 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPT ED IGICTGIFRTDGVP EDIVKLVDTFPGQSIDFFGALRARVYD+EVRKWA
Sbjct: 301 KFYWAPTREDRIGICTGIFRTDGVPTEDIVKLVDTFPGQSIDFFGALRARVYDNEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVE IGK+LVNSKEGPPTF+QPKMTLEKLLEYGNMLI EQENVKRVKLA+KYLKEAA
Sbjct: 361 AGVGVESIGKNLVNSKEGPPTFNQPKMTLEKLLEYGNMLIMEQENVKRVKLADKYLKEAA 420

Query: 421 LGDANEDVLQFQT 433
           LGDANEDV+QF+T
Sbjct: 421 LGDANEDVVQFET 431

BLAST of Cp4.1LG05g09380 vs. ExPASy TrEMBL
Match: A0A6J1JH51 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X9 OS=Cucurbita maxima OX=3661 GN=LOC111484446 PE=4 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 1.58e-287
Identity = 396/433 (91.45%), Postives = 416/433 (96.07%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MA TV+T+GAVNRT+LNNSNYG LVPNSAFLG RLK  SS+ +TTS MVIGNFKIVAEY+
Sbjct: 1   MAATVSTVGAVNRTTLNNSNYGGLVPNSAFLGSRLK-VSSK-STTSKMVIGNFKIVAEYN 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDI RGKGLADPLFQAPMGTGTHNA++SSYEY+SAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDITRGKGLADPLFQAPMGTGTHNAVLSSYEYVSAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YN DNN+DGFYIAPAFMDKL+VHIVKNFM+LPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNFDNNMDGFYIAPAFMDKLMVHIVKNFMNLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CL INDLDAGAGRFGG T
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAGAGRFGGAT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM+
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMD 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPT ED IGICTGIFRTDGVP EDIVKLVDTFPGQSIDFFGALRARVYD+EVRKWA
Sbjct: 301 KFYWAPTREDRIGICTGIFRTDGVPTEDIVKLVDTFPGQSIDFFGALRARVYDNEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVE IGK+LVNSKEGPPTF+QPKMTLEKLLEYGNMLI EQENVKRVKLA+KYLKEAA
Sbjct: 361 AGVGVESIGKNLVNSKEGPPTFNQPKMTLEKLLEYGNMLIMEQENVKRVKLADKYLKEAA 420

Query: 421 LGDANEDVLQFQT 433
           LGDANEDV+QF+T
Sbjct: 421 LGDANEDVVQFET 431

BLAST of Cp4.1LG05g09380 vs. ExPASy TrEMBL
Match: A0A6J1FWI3 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC111448804 PE=4 SV=1)

HSP 1 Score: 794 bits (2051), Expect = 1.64e-287
Identity = 397/434 (91.47%), Postives = 416/434 (95.85%), Query Frame = 0

Query: 1   MATTVATIGAVNRTSLNNSNYGDLVPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVAEYD 60
           MA TV+TIGAVNRT+LNNSNYG LVPNSAFLG RLK  SS+ +TTS MVIGNFKIVAEY+
Sbjct: 1   MAATVSTIGAVNRTTLNNSNYGGLVPNSAFLGSRLK-VSSK-STTSKMVIGNFKIVAEYN 60

Query: 61  EEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAGLRQ 120
           EEKQTEKDKWRGLAFDTSDDQQDI RGKGLADPLFQAPMGTGTHNA++SSYEY+SAGLRQ
Sbjct: 61  EEKQTEKDKWRGLAFDTSDDQQDITRGKGLADPLFQAPMGTGTHNAVLSSYEYVSAGLRQ 120

Query: 121 YNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180
           YN DNN+DGFYIAPAFMDKL+VHIVKNFM+LPNIKVPLILGIWGGKGQGKSFQCELVFAK
Sbjct: 121 YNFDNNMDGFYIAPAFMDKLMVHIVKNFMNLPNIKVPLILGIWGGKGQGKSFQCELVFAK 180

Query: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFGGTT 240
           MGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CL INDLDAGAGRFGG T
Sbjct: 181 MGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAGAGRFGGAT 240

Query: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRME 300
           QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM+
Sbjct: 241 QYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMD 300

Query: 301 KFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA 360
           KFYWAPT ED IGICTGIFRTDGVP EDIVKLVDTFPGQSIDFFGALRARVYD+EVRKWA
Sbjct: 301 KFYWAPTREDRIGICTGIFRTDGVPTEDIVKLVDTFPGQSIDFFGALRARVYDNEVRKWA 360

Query: 361 AGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLKEAA 420
           AGVGVE IGK+LVNSKEGPPTF+QPKMTLEKLL YGNMLI EQENVKRVKLA+KYLKEAA
Sbjct: 361 AGVGVESIGKNLVNSKEGPPTFNQPKMTLEKLLGYGNMLIMEQENVKRVKLADKYLKEAA 420

Query: 421 LGDANEDVLQFQTS 434
           LGDANEDV+QF+TS
Sbjct: 421 LGDANEDVVQFETS 432

BLAST of Cp4.1LG05g09380 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 682.2 bits (1759), Expect = 2.8e-196
Identity = 339/440 (77.05%), Postives = 378/440 (85.91%), Query Frame = 0

Query: 1   MATTVATIGAVNRT--SLNNSNYGDL-VPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVA 60
           MA  V+T+GA+NR   SLN S  G +  P S FLG+++    SRF  ++    G+FK++A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  EYDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAG 120
              E+KQT+ D+WRGLA+DTSDDQQDI RGKG+ D +FQAPMGTGTH+A++SSYEY+S G
Sbjct: 61  -VKEDKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYVSQG 120

Query: 121 LRQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELV 180
           LRQYNLDN +DGFYIAPAFMDKLVVHI KNF++LPNIKVPLILGIWGGKGQGKSFQCELV
Sbjct: 121 LRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQCELV 180

Query: 181 FAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFG 240
            AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCL INDLDAGAGR G
Sbjct: 181 MAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAGRMG 240

Query: 241 GTTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDG 300
           GTTQYTVNNQMVNATLMNIADNPTNVQLPG+YNKEEN RVPII TGNDFSTLYAPLIRDG
Sbjct: 241 GTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLIRDG 300

Query: 301 RMEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVR 360
           RMEKFYWAPT ED IG+C GIFRTD +  EDIV LVD FPGQSIDFFGALRARVYDDEVR
Sbjct: 301 RMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVR 360

Query: 361 KWAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLK 420
           K+   +GVE+IGK LVNS+EGPP F+QP+MT EKL+EYGNML+ EQENVKRV+LAE YL 
Sbjct: 361 KFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYLS 420

Query: 421 EAALGDANEDVLQFQTSLGK 438
           +AALGDAN D +   T  GK
Sbjct: 421 QAALGDANADAIGRGTFYGK 438

BLAST of Cp4.1LG05g09380 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 682.2 bits (1759), Expect = 2.8e-196
Identity = 339/440 (77.05%), Postives = 378/440 (85.91%), Query Frame = 0

Query: 1   MATTVATIGAVNRT--SLNNSNYGDL-VPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVA 60
           MA  V+T+GA+NR   SLN S  G +  P S FLG+++    SRF  ++    G+FK++A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  EYDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAG 120
              E+KQT+ D+WRGLA+DTSDDQQDI RGKG+ D +FQAPMGTGTH+A++SSYEY+S G
Sbjct: 61  -VKEDKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYVSQG 120

Query: 121 LRQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELV 180
           LRQYNLDN +DGFYIAPAFMDKLVVHI KNF++LPNIKVPLILGIWGGKGQGKSFQCELV
Sbjct: 121 LRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQCELV 180

Query: 181 FAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFG 240
            AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCL INDLDAGAGR G
Sbjct: 181 MAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAGRMG 240

Query: 241 GTTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDG 300
           GTTQYTVNNQMVNATLMNIADNPTNVQLPG+YNKEEN RVPII TGNDFSTLYAPLIRDG
Sbjct: 241 GTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLIRDG 300

Query: 301 RMEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVR 360
           RMEKFYWAPT ED IG+C GIFRTD +  EDIV LVD FPGQSIDFFGALRARVYDDEVR
Sbjct: 301 RMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVR 360

Query: 361 KWAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLK 420
           K+   +GVE+IGK LVNS+EGPP F+QP+MT EKL+EYGNML+ EQENVKRV+LAE YL 
Sbjct: 361 KFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYLS 420

Query: 421 EAALGDANEDVLQFQTSLGK 438
           +AALGDAN D +   T  GK
Sbjct: 421 QAALGDANADAIGRGTFYGK 438

BLAST of Cp4.1LG05g09380 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 682.2 bits (1759), Expect = 2.8e-196
Identity = 339/440 (77.05%), Postives = 378/440 (85.91%), Query Frame = 0

Query: 1   MATTVATIGAVNRT--SLNNSNYGDL-VPNSAFLGRRLKNFSSRFTTTSNMVIGNFKIVA 60
           MA  V+T+GA+NR   SLN S  G +  P S FLG+++    SRF  ++    G+FK++A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTV-SRFAQSNKKSNGSFKVLA 60

Query: 61  EYDEEKQTEKDKWRGLAFDTSDDQQDIARGKGLADPLFQAPMGTGTHNAIMSSYEYISAG 120
              E+KQT+ D+WRGLA+DTSDDQQDI RGKG+ D +FQAPMGTGTH+A++SSYEY+S G
Sbjct: 61  -VKEDKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYEYVSQG 120

Query: 121 LRQYNLDNNVDGFYIAPAFMDKLVVHIVKNFMSLPNIKVPLILGIWGGKGQGKSFQCELV 180
           LRQYNLDN +DGFYIAPAFMDKLVVHI KNF++LPNIKVPLILGIWGGKGQGKSFQCELV
Sbjct: 121 LRQYNLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKSFQCELV 180

Query: 181 FAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLLINDLDAGAGRFG 240
            AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCL INDLDAGAGR G
Sbjct: 181 MAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDAGAGRMG 240

Query: 241 GTTQYTVNNQMVNATLMNIADNPTNVQLPGLYNKEENPRVPIIVTGNDFSTLYAPLIRDG 300
           GTTQYTVNNQMVNATLMNIADNPTNVQLPG+YNKEEN RVPII TGNDFSTLYAPLIRDG
Sbjct: 241 GTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYAPLIRDG 300

Query: 301 RMEKFYWAPTHEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVR 360
           RMEKFYWAPT ED IG+C GIFRTD +  EDIV LVD FPGQSIDFFGALRARVYDDEVR
Sbjct: 301 RMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARVYDDEVR 360

Query: 361 KWAAGVGVERIGKSLVNSKEGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLK 420
           K+   +GVE+IGK LVNS+EGPP F+QP+MT EKL+EYGNML+ EQENVKRV+LAE YL 
Sbjct: 361 KFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQLAETYLS 420

Query: 421 EAALGDANEDVLQFQTSLGK 438
           +AALGDAN D +   T  GK
Sbjct: 421 QAALGDANADAIGRGTFYGK 438

BLAST of Cp4.1LG05g09380 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 3.5e-90
Identity = 166/295 (56.27%), Postives = 220/295 (74.58%), Query Frame = 0

Query: 130 FYIAPAFMDKLVVHIVKNFMSLP-NIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMM 189
           +YIAP+F+DK+ VHIVKN+++   NIK+PLILGIWGGKGQGK+FQ EL+F  MG+ P++M
Sbjct: 136 YYIAPSFLDKVAVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQTELIFKTMGVEPVIM 195

Query: 190 SAGELESGNAGEPAKLIRQRYREAADLIK-KGKMCCLLINDLDAGAGRFGGTTQYTVNNQ 249
           SAGELES  AGEP +LIR RYR A+ +I+ +GKM  L+IND+DAG GRF G TQ TVNNQ
Sbjct: 196 SAGELESDRAGEPGRLIRDRYRTASQVIQNQGKMSVLMINDIDAGLGRF-GETQMTVNNQ 255

Query: 250 MVNATLMNIADNPTNVQLPGLYNKEEN-PRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAP 309
           +V  TLMN+ADNPT V +   + + +   RVP+IVTGNDFSTLYAPLIR+GRMEKFYW P
Sbjct: 256 IVVGTLMNLADNPTRVSVGQEWREADMVNRVPLIVTGNDFSTLYAPLIREGRMEKFYWQP 315

Query: 310 THEDCIGICTGIFRTDGVPIEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWA--AGVG 369
           T ED + I + ++  DG+  +D++ +VD FP Q++DF+GALR+R YD  + KW   AG G
Sbjct: 316 TREDIVNIVSRMYEKDGISRKDVISIVDKFPNQALDFYGALRSRTYDRSILKWVDEAG-G 375

Query: 370 VERIGKSLVNSK--EGPPTFDQPKMTLEKLLEYGNMLIKEQENVKRVKLAEKYLK 418
           +E +GK L+  K  +  P F  P+ T+E LLE G  LI EQ+ +   KL+++Y+K
Sbjct: 376 METLGKVLLRRKKTQEVPQFTAPEQTVEALLESGYSLINEQKLIMETKLSKEYMK 428