Cp4.1LG05g08460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g08460
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Peptidyl-tRNA hydrolase family protein
Location	Cp4.1LG05: 5553318 .. 5558162 (+)
RNA-Seq Expression	Cp4.1LG05g08460
Synteny	Cp4.1LG05g08460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCAATAAGTTATCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAGTTCAGAGGAACCAGGCACAATGTAATTACTGCATTTTGATTTTGAAATGAGAGATATTAGTTCGTTCATTCTTATTTTGGTACAGTACAGGTAGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGTGAAGGCATGTTCACTTTTTTATTTACAGGACTTGTTGTTTTTCTTTTGATTGCATGAAGTTGAAGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACTTGAGTGGTGAATCTGTATGTGATCGAGAAACTCCTTTTGGCTTCTGTCTTGTTATAATTTAGGTTCCATTTAGTCGTCGTTTTTTTATTGCTCGAGTTCACGAATTCATTCGAGTGAATTCATCTTTCAACACGATGACTTCGAGGGAAAAAAAATCTTGGTATTCGATTTAAGATCTACTATGAGCAGTTCTTGTGATGGTTATTGTAGCTAGTTCTTTACTGAGATTGGTTTGATGCCATAAGTATTGTGTTTTGTCGTCATATTGCAGGCAGGACCGCTCGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGGTAAATCCTTATGTGAATTTGAATCATTTTTTTAATTGCATAGTTCTTAAATGATCAAAGGCATGGTATTATTTCAAGTATTTAACCTCTGCAGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAACGACTCCCACAACGGGTATCATTGATCTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATCTAACCCAAATGATTCTACATGCAACCTAAACTACATAGAATTGCAAAAAACTTAGCCATTGGCTAAAGGAAGTATAGATGCTTCTTTTACTGTATTTTCCAAGTCCCCTTACAAATACAATATACATGGCTTTATATAGTCTCAAAATGGAACTAGCATTCCAGAGTGGTAACATTCATACTTAATGACCATAATTAACCATTGATGTAATTGTAACCTAAATTAAATAAAAAGTTATAAAATACATTAATGAAATACAATAACTCTGAATTACTCTAAATTATAATCCACCCATGAATTGAAATATCTTTTTGATAGTTACAACAATATTTTACCTCGACCCATAAAATTTTTACCTCGTCTAAAATTATTTTGGTCTATTTACTCTCGACGTTGAGACGTTGATTCATTTTGACCCCGTAATTTTGGTATAATTTGATTTGGGTAGCATGTGGAACAATTTTTGTCCTAGATGCAAAATTTTAAAAAAACAAGGGATAAAAAGGAAAATTAAGGGGAAATATAGGGACCAATAAAGCAAAAGCTAAAAAATTACAGGGACCAAACTGTGAATTAAAAAATTACAGGGACCAAACTGTCAATTAAAGAAAATTTCCACTTACTAGGTTTCTCTCCCTGATTTCACCATTTTCATGGCTTCTTCTTGAAAGACATTCGTCCACCAAGGTATCTCTCTCTTTCATTCTCTTCATTTCGTCTTTGATTTTTCAATTTTCCGGTTGAATTCAAGGTCTGGTACCACCTTCGGAACGGCTGCGATCGGCGATAACAATAGGCGGCGGGGGCGAAATGAAACAGCCGCTAGTGGCTGCCGCATCGCACTGTTCGTCCTTTTTCTTCTTTTTTGCTGTTAATCCTCACATTGTATTTGTAATCTGCGTTAGAATCAACTCAGGGACATGACTCGAGTGCGGAATTGAATTGCCAATAAATTTTTGTTTATTACTTTTACATTTTGGGGTTGCTTCTTTTGTTCTTAATTGTTTAGGAGCTGATCGAATTTTTTGGTTTTCAGTCAATAGGGAAGTGTAAAATGTTCAATAAGTTATCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAGTTCAGAGGAACCAGGCACAATGTAATTACTGCATTTTGATTTTGAAATGAGAGATATTAGTTCGTTCATTCTTATTTTGGTACAGTACAGGTAGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGTGAAGGCATGTTCACTTTTTTATTTACAGGACTTGTTGTTTTTCTTTTGATTGCATGAAGTTGAAGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACTTGAGTGGTGAATCTGTATGTGATCGAGAAACTCCTTTTGGCTTCTGTCTTGTTATAATTTAGGTTCCATTTAGTCGTCGTTTTTTTATTGCTCGAGTTCACGAATTCATTCGAGTGAATTCATCTTTCAACACGATGACTTCGAGGGAAAAAAAATCTTGGTATTCGATTTAAGATCTACTATGAGCAGTTCTTGTGATGGTTATTGTAGCTAGTTCTTTACTGAGATTGGTTTGATGCCATAAGTATTGTGTTTTGTCGTCATATTGCAGGCAGGACCGCTCGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGGTAAATCCTTATGTGAATTTGAATCATTTTTTTAATTGCATAGTTCTTAAATGATCAAAGGCATGGTATTATTTCAAGTATTTAACCTCTGCAGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAACGACTCCCACAACGGGTATCATTGATCTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNGTAGCCTATTTCCACTCTATAGCCACAAGCTAGGTTGCCTCGTGTTTTCACACCTCCTCTGTGACATTGACTTACATTTGACTGAATTCGATTGGTTGTTTTCATTTGAAAAGAGAAAGTGTGAGATCCCACATCGATTGGAGAGGGCAACGAAGCATTCCTTATAAAGGTGTGAAAACCTCTCCATGACAGATGCGTTTTAAAAAGGGAAAGCTGAAAGAGAACAGTATTTGCTAGTGGTGGGCTTGGGCTGTTACAAATGGTATCAAAGCTAGACACCGAGCGGTGTGCCAACAAGGACACTGGCCCCCAAGGGGAGTGGATTGTGAAATTCCACATCGGTTCGAGAGGGGAATGAAATATTCCTTATTAGGGTGTGGAAACCTCTCCTTAACAAACGCGTTTTAAAAACCTTGAGGGGAAGCCCAGAAAGGAAAGCCGAAAAAGGACAATATCTGCTAGTAGGGAGCTTAAACTGTTACAAAAAAGAGTCCCAATAAGAGTATGTAAGATGGTTTACTATATCAACATTTGATTTAGCGTCCAGCATGCTAGAGAGATGATGATAACTACATCCTATTTGGATTGCTTTTTTATGTTTGGTCATGTTAGTTAATCGTTCCATTATCCATACGCGCTTGATGAATCGAACAAAGCATTTGTGGGGTCAAAGGTACCATACATAAAGTCCTAATACAACTTTCAGAATGTTAGCTGCTGCCATGAAGTTTTATCAACGTGATTCCTCTAAACATCATGTTTAGCTTCAAGCTTAGCTTGAATTCTTTTTTTGTAACGACATACTACTAATCGTTCATGCTTATTCTTTCTTTAGTAAAACCTTCACGACTTCTCGTCCATTCTATCGCCTTTTCTGGTACATCTTGAAATGGATTTTTATTTTAGGCTGAAGAGTGTCATTTATCACTTTCGAGGGAACCGAGAGTTTGCTAGGTTCAGAATTGGTAAGTATTGTTGCCTATGCTATTTTGTGGTCTTTTATGTGTTTCTCCTCCGTTCTTAGAACGTTTTCTTTATAGGCATTGGAAGGCCACCTGGTCAAATGGATCCCAAAGCGTTCTTGCTTCAAAAGTTCAATAACACAGCTCAAGAACGAGTGAGTCTTTTGCTGATTATACTACACGAAACTTATGGTTGAAACCTTTCTATCTTTCCTTGAGCCCCCATGAAATTTCATTTGCAAATTACTGCTTACCTTATATTCACTCTCCATTCTTTTGCTTGAAAAATATATTTTGGACCCTCTTGAATCCCACTTTTAATATTTGGCAAGTATAGTTTGTGTCTTACAAACCTCGATTACATTATGATTATTACCCATCTCGGCATAACGCTTCCCTTATTTTTTGTTTAAAAAGATTGATACTGCATTGCAAGAGGGGGTCGGTGCATTGAAGCTCCTATTGTCTCACGGTTTATCCGAGACTGCGAGACGTTTCAACCACGAACAGAAATACAAGCATATAAGGCTACAGACCATGCCAACATGATGAGCCTACTATGCGTATTTCCATTGCTAATGAAACATACAAATTCTACCAAGTACTCAAAGGGAAGGGGAGCACAGAGGCAGAGTGGATGGGCTCCTGAAGGTACAATGACTTTGTGTTCTAAGATATTGGGCCAGAAATGGAGAATTTAGAGGACGTTTTGAGATATCCTGTCTCTATATGTAATTATTTTATGCTTACAACTGATAAACTTTATAAGCTTAGGTACTTGAAAAATTGCCTCATGATTAATTTATATTTATAGAAGTGGAGCCGATTGTTTATGTATTTTTTTTTCTTGTTCGAGTTCATAAGCTCTCTTAAAGAGAATCAATTTAGACGTCTTTGGTCA

mRNA sequence

ATGTTCAATAAGTTATCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAGTTCAGAGGAACCAGGCACAATGTAGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACTTGAGTGGTGAATCTGCAGGACCGCTCGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGGAGCTGATCGAATTTTTTGGTTTTCAGTCAATAGGGAAGTGTAAAATGTTCAATAAGTTATCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAGTTCAGAGGAACCAGGCACAATGTAGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACTTGAGTGGTGAATCTGCAGGACCGCTCGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAACGACTCCCACAACGGGCTGAAGAGTGTCATTTATCACTTTCGAGGGAACCGAGAGTTTGCTAGGTTCAGAATTGGCATTGGAAGGCCACCTGGTCAAATGGATCCCAAAGCGTTCTTGCTTCAAAAGTTCAATAACACAGCTCAAGAACGAATTGATACTGCATTGCAAGAGGGGGTCGGTGCATTGAAGCTCCTATTGTCTCACGGTTTATCCGAGACTGCGAGACGTTTCAACCACGAACAGAAATACAAGCATATAAGGCTACAGACCATGCCAACATGATGAGCCTACTATGCGTATTTCCATTGCTAATGAAACATACAAATTCTACCAAGTACTCAAAGGGAAGGGGAGCACAGAGGCAGAGTGGATGGGCTCCTGAAGGTACAATGACTTTGTGTTCTAAGATATTGGGCCAGAAATGGAGAATTTAGAGGACGTTTTGAGATATCCTGTCTCTATATGTAATTATTTTATGCTTACAACTGATAAACTTTATAAGCTTAGGTACTTGAAAAATTGCCTCATGATTAATTTATATTTATAGAAGTGGAGCCGATTGTTTATGTATTTTTTTTTCTTGTTCGAGTTCATAAGCTCTCTTAAAGAGAATCAATTTAGACGTCTTTGGTCA

Coding sequence (CDS)

ATGTTCAATAAGTTATCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAGTTCAGAGGAACCAGGCACAATGTAGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACTTGAGTGGTGAATCTGCAGGACCGCTCGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGGAGCTGATCGAATTTTTTGGTTTTCAGTCAATAGGGAAGTGTAAAATGTTCAATAAGTTATCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAGTTCAGAGGAACCAGGCACAATGTAGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACTTGAGTGGTGAATCTGCAGGACCGCTCGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAACGACTCCCACAACGGGCTGAAGAGTGTCATTTATCACTTTCGAGGGAACCGAGAGTTTGCTAGGTTCAGAATTGGCATTGGAAGGCCACCTGGTCAAATGGATCCCAAAGCGTTCTTGCTTCAAAAGTTCAATAACACAGCTCAAGAACGAATTGATACTGCATTGCAAGAGGGGGTCGGTGCATTGAAGCTCCTATTGTCTCACGGTTTATCCGAGACTGCGAGACGTTTCAACCACGAACAGAAATACAAGCATATAAGGCTACAGACCATGCCAACATGA

Protein sequence

MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVELIEFFGFQSIGKCKMFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT

Homology

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BLAST of Cp4.1LG05g08460 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.2e-87
Identity = 153/216 (70.83%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           M ++LS+R +CT+   RPWLF+GLGNPG+K++GTRHN+GFEMIDVFAESVGI MN ++ K
Sbjct: 1   MLSRLSRRCYCTSSVHRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFK 60

Query: 187 AVF--GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           A+   GFV D+PV LAKPQTYMNLSGES+GPLAAYYKLPLNRVLV HDDM LPCGVLRL+
Sbjct: 61  AIMGQGFVADLPVILAKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQ 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
             GG+  HNGLKSV+ HFRGNREFAR RIGIG+PPGQMDPKAFLLQKF+  A+ER+D AL
Sbjct: 121 EKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTM 341
            EGV ALKL+L+    E+ R FN EQKYKH++  T+
Sbjct: 181 AEGVDALKLVLAKDFGESWRLFNVEQKYKHLKQHTI 216

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy Swiss-Prot
Match: Q9M5P4 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.5e-61
Identity = 110/196 (56.12%), Postives = 143/196 (72.96%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL  GLGNPG K+ GTRHNVGFEM+D  A   GI MN I  K++ G   +G++PV + K
Sbjct: 61  PWLIAGLGNPGNKYYGTRHNVGFEMVDRIAAEEGITMNTIQSKSLLGIGSIGEVPVLVVK 120

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQ+YMN SGE+ GPLAAYY++PL  +L+ +DD +LP GVLRL+  GG+  HNGL++VI H
Sbjct: 121 PQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEH 180

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G REF R  IGIG PPG+MDP+AFLLQKF++  + +IDTAL++GV A++ L+  G S 
Sbjct: 181 LDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSG 240

Query: 324 TARRFNHEQKYKHIRL 338
           +  RFN  QKYK  R+
Sbjct: 241 STERFNLVQKYKFHRV 256

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BLAST of Cp4.1LG05g08460 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.2e-59
Identity = 109/192 (56.77%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL VGLGNPG K+ GTRHNVGFEMIDV A   G+ MN I  KA+ G   + D+P+ LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQTYMN SGES G LA++Y++PL  +L+ +D+M LP GVLRL+  GG   HNG+KSV+ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G R F R  IGIG+PPG MD KAFLLQKF+   Q++I+ AL++G  A+K L+ +G ++
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 324 TARRFNHEQKYK 334
              RFN  QKYK
Sbjct: 225 GISRFNLVQKYK 236

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy Swiss-Prot
Match: Q9FKN4 (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2A PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.6e-58
Identity = 107/192 (55.73%), Postives = 134/192 (69.79%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL VGLGNPG K+ GTRHN+GFEMID  A +  I MN I  KA+ G   VG++P+ L K
Sbjct: 51  PWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQSKALVGIGSVGEVPILLVK 110

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQ YMN SGES GPLAAYY++PL  +L+ +DDM L  GVLRL+  GG+  HNGLK+V  H
Sbjct: 111 PQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEH 170

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G R + R  IGIG PPG MD KAFLLQKF+   ++++D  L++GV  +K L+  G S+
Sbjct: 171 LNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSD 230

Query: 324 TARRFNHEQKYK 334
           +  RFN  QKYK
Sbjct: 231 SISRFNLGQKYK 242

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy Swiss-Prot
Match: Q5ZCL8 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 105/192 (54.69%), Postives = 139/192 (72.40%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL  GLGNPG K+ GTRHNVGFEM+D  A   GI MN I  K++ G   +G++PV L K
Sbjct: 64  PWLIAGLGNPGSKYHGTRHNVGFEMVDRIARDEGITMNTIQSKSLLGIGSIGEVPVLLVK 123

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQ+Y+N SGE+ GPLAAYY++PL  +LV +D+M+LP GVLRL+  GG+  HNGL++V+  
Sbjct: 124 PQSYINYSGEAIGPLAAYYQVPLRHILVMYDEMSLPNGVLRLQRKGGHGRHNGLQNVMEC 183

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
              +RE  R  IGIG PPG+MD +AFLLQKF++  + +IDTAL++GV A++ L+  G S 
Sbjct: 184 LDSSRELPRLSIGIGSPPGKMDTRAFLLQKFSSEERLQIDTALEQGVDAVRTLVLKGFSG 243

Query: 324 TARRFNHEQKYK 334
           +  RFN  QKYK
Sbjct: 244 SIERFNLVQKYK 255

[Close]

BLAST of Cp4.1LG05g08460 vs. NCBI nr
Match: XP_023532821.1 (peptidyl-tRNA hydrolase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532822.1 peptidyl-tRNA hydrolase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 446 bits (1148), Expect = 3.50e-156
Identity = 216/218 (99.08%), Postives = 216/218 (99.08%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
           QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT
Sbjct: 181 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 218

[Close]

BLAST of Cp4.1LG05g08460 vs. NCBI nr
Match: XP_022961338.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita moschata] >XP_022961339.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita moschata] >KAG6590274.1 Peptidyl-tRNA hydrolase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 440 bits (1131), Expect = 1.36e-153
Identity = 214/218 (98.17%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGGNDSHNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
           QEGVGALKLLLS GLSETARRFNHEQKYKHIRLQTMPT
Sbjct: 181 QEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 218

[Close]

BLAST of Cp4.1LG05g08460 vs. NCBI nr
Match: XP_022988207.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima] >XP_022988208.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 437 bits (1125), Expect = 1.11e-152
Identity = 212/218 (97.25%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGGNDSHNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
           QEG+GALKLLLS GLSETAR FNHEQKYKHIRLQTMPT
Sbjct: 181 QEGIGALKLLLSLGLSETARHFNHEQKYKHIRLQTMPT 218

[Close]

BLAST of Cp4.1LG05g08460 vs. NCBI nr
Match: KAG7033441.1 (Peptidyl-tRNA hydrolase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 437 bits (1125), Expect = 1.38e-152
Identity = 214/224 (95.54%), Postives = 214/224 (95.54%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 187 AVFG--------FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 246
           AVFG        FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC
Sbjct: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120

Query: 247 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQE 306
           GVLRLRHNGGNDSHNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 307 RIDTALQEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
           RIDTALQEGVGALKLLLS GLSETARRFNHEQKYKHIRLQTMPT
Sbjct: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 224

[Close]

BLAST of Cp4.1LG05g08460 vs. NCBI nr
Match: XP_038880695.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 426 bits (1094), Expect = 5.82e-148
Identity = 205/217 (94.47%), Postives = 212/217 (97.70%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKL+KRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPM+R+HCK
Sbjct: 1   MFNKLTKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMSRLHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRL+
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLQ 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGG+D HNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGHDYHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMP 341
           QEGVGALKL+LS GLSE+ARRFNHEQKYKHIRLQTMP
Sbjct: 181 QEGVGALKLVLSLGLSESARRFNHEQKYKHIRLQTMP 217

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy TrEMBL
Match: A0A6J1HDR5 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461856 PE=3 SV=1)

HSP 1 Score: 440 bits (1131), Expect = 6.57e-154
Identity = 214/218 (98.17%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGGNDSHNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
           QEGVGALKLLLS GLSETARRFNHEQKYKHIRLQTMPT
Sbjct: 181 QEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 218

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy TrEMBL
Match: A0A6J1JLL3 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485515 PE=3 SV=1)

HSP 1 Score: 437 bits (1125), Expect = 5.38e-153
Identity = 212/218 (97.25%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGGNDSHNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
           QEG+GALKLLLS GLSETAR FNHEQKYKHIRLQTMPT
Sbjct: 181 QEGIGALKLLLSLGLSETARHFNHEQKYKHIRLQTMPT 218

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy TrEMBL
Match: A0A1S3BPZ7 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492429 PE=3 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 8.07e-148
Identity = 203/217 (93.55%), Postives = 210/217 (96.77%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MFNKL+KRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGF+MIDVFAESVGIPMNR+HCK
Sbjct: 1   MFNKLTKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFQMIDVFAESVGIPMNRVHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAA+YKLPLNRVLVFHDDMNLPCGVLRL+
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAHYKLPLNRVLVFHDDMNLPCGVLRLQ 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGG+D HNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGHDYHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMP 341
           QEGVGALK LLSHGLSE+ R FNHEQKYKHIRLQTMP
Sbjct: 181 QEGVGALKRLLSHGLSESVRHFNHEQKYKHIRLQTMP 217

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy TrEMBL
Match: A0A0A0LWX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G600200 PE=3 SV=1)

HSP 1 Score: 416 bits (1070), Expect = 1.27e-144
Identity = 201/217 (92.63%), Postives = 207/217 (95.39%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MF+KL+KRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNR+HCK
Sbjct: 1   MFSKLTKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRVHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           AVFG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDM LPCGVLRL+
Sbjct: 61  AVFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMTLPCGVLRLQ 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
           HNGG+  HNGLKSVI HFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL
Sbjct: 121 HNGGHGYHNGLKSVISHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMP 341
           QEGVGALKLLLSH L E+AR FNHEQKYKHIRLQTMP
Sbjct: 181 QEGVGALKLLLSHSLFESARHFNHEQKYKHIRLQTMP 217

[Close]

BLAST of Cp4.1LG05g08460 vs. ExPASy TrEMBL
Match: A0A6J1D205 (peptidyl-tRNA hydrolase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016566 PE=3 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 5.98e-143
Identity = 199/218 (91.28%), Postives = 209/218 (95.87%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           MF+KL+KRGFCTA+S RPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIP+NR+HCK
Sbjct: 1   MFSKLTKRGFCTALSPRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPINRVHCK 60

Query: 187 AVFG--FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           A+FG  FVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR
Sbjct: 61  ALFGEGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
            +GG+D HNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTA+ERIDTAL
Sbjct: 121 PDGGHDYHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTARERIDTAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 342
            EGVGALK LLS GLSE+ARRFNHEQKYKHIRLQTMPT
Sbjct: 181 LEGVGALKDLLSLGLSESARRFNHEQKYKHIRLQTMPT 218

[Close]

BLAST of Cp4.1LG05g08460 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 324.7 bits (831), Expect = 8.9e-89
Identity = 153/216 (70.83%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 127 MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 186
           M ++LS+R +CT+   RPWLF+GLGNPG+K++GTRHN+GFEMIDVFAESVGI MN ++ K
Sbjct: 1   MLSRLSRRCYCTSSVHRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFK 60

Query: 187 AVF--GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLR 246
           A+   GFV D+PV LAKPQTYMNLSGES+GPLAAYYKLPLNRVLV HDDM LPCGVLRL+
Sbjct: 61  AIMGQGFVADLPVILAKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQ 120

Query: 247 HNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTAL 306
             GG+  HNGLKSV+ HFRGNREFAR RIGIG+PPGQMDPKAFLLQKF+  A+ER+D AL
Sbjct: 121 EKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKAL 180

Query: 307 QEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTM 341
            EGV ALKL+L+    E+ R FN EQKYKH++  T+
Sbjct: 181 AEGVDALKLVLAKDFGESWRLFNVEQKYKHLKQHTI 216

[Close]

BLAST of Cp4.1LG05g08460 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-60
Identity = 109/192 (56.77%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL VGLGNPG K+ GTRHNVGFEMIDV A   G+ MN I  KA+ G   + D+P+ LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQTYMN SGES G LA++Y++PL  +L+ +D+M LP GVLRL+  GG   HNG+KSV+ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G R F R  IGIG+PPG MD KAFLLQKF+   Q++I+ AL++G  A+K L+ +G ++
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 324 TARRFNHEQKYK 334
              RFN  QKYK
Sbjct: 225 GISRFNLVQKYK 236

[Close]

BLAST of Cp4.1LG05g08460 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-60
Identity = 109/192 (56.77%), Postives = 138/192 (71.88%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL VGLGNPG K+ GTRHNVGFEMIDV A   G+ MN I  KA+ G   + D+P+ LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQTYMN SGES G LA++Y++PL  +L+ +D+M LP GVLRL+  GG   HNG+KSV+ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G R F R  IGIG+PPG MD KAFLLQKF+   Q++I+ AL++G  A+K L+ +G ++
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 324 TARRFNHEQKYK 334
              RFN  QKYK
Sbjct: 225 GISRFNLVQKYK 236

[Close]

BLAST of Cp4.1LG05g08460 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.3e-59
Identity = 107/192 (55.73%), Postives = 134/192 (69.79%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL VGLGNPG K+ GTRHN+GFEMID  A +  I MN I  KA+ G   VG++P+ L K
Sbjct: 55  PWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQSKALVGIGSVGEVPILLVK 114

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQ YMN SGES GPLAAYY++PL  +L+ +DDM L  GVLRL+  GG+  HNGLK+V  H
Sbjct: 115 PQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEH 174

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G R + R  IGIG PPG MD KAFLLQKF+   ++++D  L++GV  +K L+  G S+
Sbjct: 175 LNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSD 234

Query: 324 TARRFNHEQKYK 334
           +  RFN  QKYK
Sbjct: 235 SISRFNLGQKYK 246

[Close]

BLAST of Cp4.1LG05g08460 vs. TAIR 10
Match: AT5G38290.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.3e-59
Identity = 107/192 (55.73%), Postives = 134/192 (69.79%), Query Frame = 0


Query: 144 PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGF--VGDIPVFLAK 203
           PWL VGLGNPG K+ GTRHN+GFEMID  A +  I MN I  KA+ G   VG++P+ L K
Sbjct: 51  PWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQSKALVGIGSVGEVPILLVK 110

Query: 204 PQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYH 263
           PQ YMN SGES GPLAAYY++PL  +L+ +DDM L  GVLRL+  GG+  HNGLK+V  H
Sbjct: 111 PQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGLKNVTEH 170

Query: 264 FRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSHGLSE 323
             G R + R  IGIG PPG MD KAFLLQKF+   ++++D  L++GV  +K L+  G S+
Sbjct: 171 LNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLVEEGFSD 230

Query: 324 TARRFNHEQKYK 334
           +  RFN  QKYK
Sbjct: 231 SISRFNLGQKYK 242

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g08460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NLS8	1.2e-87	70.83	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19...	[more]
Q9M5P4	4.5e-61	56.12	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea m...	[more]
Q9LF14	3.2e-59	56.77	Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara...	[more]
Q9FKN4	4.6e-58	55.73	Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Ara...	[more]
Q5ZCL8	1.0e-57	54.69	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza...	[more]

BLAST of Cp4.1LG05g08460 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023532821.1	3.50e-156	99.08	peptidyl-tRNA hydrolase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_022961338.1	1.36e-153	98.17	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita moschata] >XP_02296...	[more]
XP_022988207.1	1.11e-152	97.25	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima] >XP_0229882...	[more]
KAG7033441.1	1.38e-152	95.54	Peptidyl-tRNA hydrolase, mitochondrial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_038880695.1	5.82e-148	94.47	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida]	[more]

BLAST of Cp4.1LG05g08460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HDR5	6.57e-154	98.17	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1JLL3	5.38e-153	97.25	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A1S3BPZ7	8.07e-148	93.55	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A0A0LWX1	1.27e-144	92.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G600200 PE=3 SV=1	[more]
A0A6J1D205	5.98e-143	91.28	peptidyl-tRNA hydrolase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Cp4.1LG05g08460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19830.1	8.9e-89	70.83	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.1	2.3e-60	56.77	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.2	2.3e-60	56.77	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.2	3.3e-59	55.73	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.1	3.3e-59	55.73	Peptidyl-tRNA hydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001328	Peptidyl-tRNA hydrolase	TIGRFAM	TIGR00447	TIGR00447	coord: 146..328 e-value: 1.0E-52 score: 176.6
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 146..327 e-value: 3.2E-54 score: 183.6 coord: 20..111 e-value: 3.8E-22 score: 79.0
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 129..338 coord: 3..111
IPR001328	Peptidyl-tRNA hydrolase	HAMAP	MF_00083	Pept_tRNA_hydro_bact	coord: 144..320 score: 30.356249
IPR036416	Peptidyl-tRNA hydrolase superfamily	GENE3D	3.40.50.1470		coord: 141..339 e-value: 6.3E-67 score: 227.0
IPR036416	Peptidyl-tRNA hydrolase superfamily	GENE3D	3.40.50.1470		coord: 6..113 e-value: 9.6E-28 score: 98.9
IPR036416	Peptidyl-tRNA hydrolase superfamily	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 144..327
IPR036416	Peptidyl-tRNA hydrolase superfamily	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 18..111
None	No IPR available	PANTHER	PTHR17224:SF4	PEPTIDYL-TRNA HYDROLASE, MITOCHONDRIAL	coord: 129..338 coord: 3..111
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01196	PEPT_TRNA_HYDROL_2	coord: 248..258
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01195	PEPT_TRNA_HYDROL_1	coord: 31..44
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01195	PEPT_TRNA_HYDROL_1	coord: 157..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g08460.1	Cp4.1LG05g08460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity