Cp4.1LG05g08040 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG05g08040 vs. ExPASy Swiss-Prot
Match: Q9SU50 (Trehalase OS=Arabidopsis thaliana OX=3702 GN=TRE1 PE=2 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 1.9e-192
Identity = 317/555 (57.12%), Postives = 415/555 (74.77%), Query Frame = 0

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDF-DLKYYVDLSLK--FDLNSTERAFDAL-SRS 108
           GPV+  + LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E AFD L S S
Sbjct: 72  GPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTSES 131

Query: 109 SNGSVPVEDLKSFISEYFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYW 168
            +  VPVE L+ F+ EYFD AG D+++ +PVDFV  P GFL  VEN EVR WA E+H  W
Sbjct: 132 HDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHGLW 191

Query: 169 KNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKG 228
           +NLS +VSD +    D HTLLPLP P IIPG+RFREVYYWDSYW+I+GL+ S+M+ TAKG
Sbjct: 192 RNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTAKG 251

Query: 229 IVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH 288
           +V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E VR ++P L+KE+
Sbjct: 252 LVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLKEY 311

Query: 289 MFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYREL 348
            FWNSG H V +R A G +H LSRYYAMWN+PRPESS+ DE+ AS F    +KQR +R++
Sbjct: 312 EFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHRDI 371

Query: 349 ASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYK 408
           A+AAESG DFS+RWMRD  + +T+ATTS++PVDLNVF+LKMELDI+ + +  GD   + +
Sbjct: 372 ATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGSDR 431

Query: 409 FLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIE 468
           F++AS  R+K   ++FWN + GQWLDYWL +   +    W A +QN N++ASNF P+WI 
Sbjct: 432 FVKASKAREKAFQTVFWNEKAGQWLDYWLSSSGEES-ETWKAENQNTNVFASNFAPIWIN 491

Query: 469 SFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSEL 528
           S  +D   +KKV+ +L+ SGL+  AGI TS  NSG+QWD PNGWAP Q MI+ GL RS +
Sbjct: 492 SINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRSSV 551

Query: 529 KEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVL 588
           KEA+ +AED+A RW+++NY+ YKK+G +HEK  V + G++G GGEY+PQTGFGWSNGV+L
Sbjct: 552 KEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGVIL 611

Query: 589 AFLEEFGWPEDQNID 600
           AFLEE+GWP   +I+
Sbjct: 612 AFLEEYGWPSHLSIE 625

BLAST of Cp4.1LG05g08040 vs. ExPASy Swiss-Prot
Match: Q9FWC1 (Probable trehalase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0521000 PE=2 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 7.6e-189
Identity = 318/567 (56.08%), Postives = 415/567 (73.19%), Query Frame = 0

Query: 42  SPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDAL 101
           +P  A  G  +    L+  L+R+Q  AL +FG  DFD K YVDL L  D +    A  AL
Sbjct: 2   APTAAVAGGGVEAEALLGLLQRVQSEALRAFGPNDFDPKLYVDLPLAADAS----AAAAL 61

Query: 102 SRSSNGSVPVEDLKSFISEYFDSAGTDMV-YSDPVDFVPRPQGFLPKVENAEVRAWALEI 161
           +     +    +++++IS YF  AG+D+V  +DP DF   P GFLP+VE AE RAWALE+
Sbjct: 62  ASLPRAAPSRGEMEAYISRYFALAGSDLVAAADPPDFERDPPGFLPRVERAEARAWALEV 121

Query: 162 HNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYD 221
           H  WK+L+R+V+  +  RPD HTLLPLP   ++PG+RFREVYYWDSYW++RGLL SKMY+
Sbjct: 122 HALWKDLTRRVAPAVAARPDRHTLLPLPGRVVVPGSRFREVYYWDSYWVVRGLLVSKMYE 181

Query: 222 TAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPAL 281
           TAK IV+NL+ +++++G VLNGAR+YYTNRSQPPLLSSMV DIY+ TGD+ FVR   P+L
Sbjct: 182 TAKDIVLNLVYLVEKYGFVLNGARSYYTNRSQPPLLSSMVLDIYMATGDMAFVRRVFPSL 241

Query: 282 IKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRL 341
           +KEH FW S  H+V V    G  H+LSRY AMWN+PRPES+ +DE+ ASK ++   K++ 
Sbjct: 242 LKEHSFWMSEVHNVAVMDNHGRVHNLSRYQAMWNKPRPESATIDEEFASK-LSTAAKEKF 301

Query: 342 YRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFC 401
           Y ++AS AE+GWDFSSRWMRD TD++TL T+ I+PVDLN FILKME DI+  A+ +G+  
Sbjct: 302 YHQVASTAETGWDFSSRWMRDSTDMTTLTTSCIIPVDLNTFILKMEQDIAFFAKLIGEST 361

Query: 402 LAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL-DNGSHKGVHAWDARSQNKNIYASNFI 461
            +  F EAS  R   I+S+ WN++  QWLDYWL  +G+ +GV+ W + SQN+ I+ASNF+
Sbjct: 362 TSEIFSEASKARHNAIDSVLWNADMEQWLDYWLPTDGNCQGVYQWKSISQNRAIFASNFV 421

Query: 462 PLWIESFYN------DSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQH 521
           PLW+ + ++      D  +  +V++SL+KSGLL  AGIATS  N+G+QWDFPNGWAP+QH
Sbjct: 422 PLWLNAQHSGLEQFVDEAKSVRVMRSLQKSGLLQPAGIATSLSNTGQQWDFPNGWAPLQH 481

Query: 522 MIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQ 581
           +I+EGL RS   EAR LAED+A RW+RTNY AYK TG MHEKYDV  CG  G GGEY PQ
Sbjct: 482 LIVEGLLRSGSGEARELAEDIATRWVRTNYDAYKATGAMHEKYDVVTCGKSGGGGEYKPQ 541

Query: 582 TGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           TGFGWSNGV+L+FL+EFGWP+D+ IDC
Sbjct: 542 TGFGWSNGVILSFLDEFGWPQDKKIDC 563

BLAST of Cp4.1LG05g08040 vs. ExPASy Swiss-Prot
Match: O43280 (Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2)

HSP 1 Score: 392.5 bits (1007), Expect = 8.5e-108
Identity = 207/523 (39.58%), Postives = 303/523 (57.93%), Query Frame = 0

Query: 74  KLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSD 133
           KL  D K +VD+ L        + F  LSR  N S+P E L++F+ E+F + G ++    
Sbjct: 46  KLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWT 105

Query: 134 PVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCII 193
           P D+   PQ FL K+ +A++RAWA ++H  WK L +K+  +++  P+  +L+    P I+
Sbjct: 106 PADWKDSPQ-FLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEHPFIV 165

Query: 194 PGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQP 253
           PG RF E YYWDSYW++ GLL S+M +T KG++ N + ++  +GHV NG R YY  RSQP
Sbjct: 166 PGGRFVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVKTYGHVPNGGRVYYLQRSQP 225

Query: 254 PLLSSMVYDIYL-RTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAM 313
           PLL+ M+ D YL  T D  F++ ++  L  E  FW     +V+V    G N+ L+RYY  
Sbjct: 226 PLLTLMM-DCYLTHTNDTAFLQENIETLALELDFWTKN-RTVSV-SLEGKNYLLNRYYVP 285

Query: 314 WNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWM---RDHTDLSTLA 373
           +  PRPES   D +LA       D++ L+ EL + AESGWDFSSRW+    +   LS + 
Sbjct: 286 YGGPRPESYSKDVELADTLPEG-DREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIR 345

Query: 374 TTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWL 433
           T+ ++PVDLN F+ + E  +SN    +G+   A K+      R   +N++ W+ + G W 
Sbjct: 346 TSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWF 405

Query: 434 DYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC-N 493
           DY L+            + +N+  Y SN  PLW   F +  +   K LK L  + +L   
Sbjct: 406 DYDLEK-----------KKKNREFYPSNLTPLWAGCFSDPGV-ADKALKYLEDNRILTYQ 465

Query: 494 AGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKK 553
            GI TS   +G+QWDFPN WAP+Q ++I GLA++ L+ A+ +A  +A  W+RTN+  Y +
Sbjct: 466 YGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQ 525

Query: 554 TGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
              M+EKYDV   G  G GGEY  Q GFGW+NGVVL  L+ +G
Sbjct: 526 KSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYG 551

BLAST of Cp4.1LG05g08040 vs. ExPASy Swiss-Prot
Match: Q9JLT2 (Trehalase OS=Mus musculus OX=10090 GN=Treh PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 7.5e-104
Identity = 199/519 (38.34%), Postives = 292/519 (56.26%), Query Frame = 0

Query: 78  DLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPVDF 137
           D K +VD+SL    +   + F  L+   N S+P E L+ F+  +F   G ++    P D+
Sbjct: 47  DDKQFVDMSLATSPDEVLQKFSELATVHNHSIPKEQLQEFVQSHFQPVGQELQSWTPEDW 106

Query: 138 VPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGAR 197
              PQ FL K+ +A +R WA E+H  WK L +K+  +++  P+  +L+    P I+PG R
Sbjct: 107 KDSPQ-FLQKISDANLRVWAEELHKIWKKLGKKMKAEVLSYPERSSLIYSKHPFIVPGGR 166

Query: 198 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 257
           F E YYWDSYW++ GLL S+M  T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 167 FVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLT 226

Query: 258 SMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGG-NHSLSRYYAMWNEP 317
            M+      T D+ F++ ++  L  E  FW     + TV    GG ++ L+RYY  +  P
Sbjct: 227 LMMDRYVAHTKDVAFLQENIGTLASELDFWTV---NRTVSVVSGGQSYVLNRYYVPYGGP 286

Query: 318 RPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTD---LSTLATTSI 377
           RPES   D +LA+  V   D++ L+ EL + AESGWDFSSRW+    D   LS++ T+ +
Sbjct: 287 RPESYRKDAELANS-VPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKM 346

Query: 378 LPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL 437
           +P DLN F+ + E  +SN    +G+   A K+      R   + ++ W+ +KG W DY L
Sbjct: 347 VPADLNAFLCQAEELMSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKGAWFDYDL 406

Query: 438 DNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC-NAGIA 497
           + G            +N   Y SN  PLW   F + S+   K LK L  S +L    GI 
Sbjct: 407 EKG-----------KKNLEFYPSNLSPLWAGCFSDPSV-ADKALKYLEDSKILTYQYGIP 466

Query: 498 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 557
           TS  N+G+QWDFPN WAP+Q ++I GLA+S     + +A  +A  W++TN+  Y +   M
Sbjct: 467 TSLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNFKVYSQKSAM 526

Query: 558 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
            EKYD+   G  G GGEY  Q GFGW+NG+ L  L+ +G
Sbjct: 527 FEKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYG 548

BLAST of Cp4.1LG05g08040 vs. ExPASy Swiss-Prot
Match: P19813 (Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.3e-103
Identity = 197/519 (37.96%), Postives = 299/519 (57.61%), Query Frame = 0

Query: 78  DLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPVDF 137
           D K +VD+ L    +   ++F  L+ + N +VP E L+ F+ E+F + G ++    P D+
Sbjct: 50  DDKQFVDMPLSTAPDQVLQSFAELAATYNNTVPREQLEKFVQEHFQAVGQELESWTPGDW 109

Query: 138 VPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGAR 197
              PQ FL K+ + ++RAWA ++H  WK L +K+  +++ +P+  +L+    P I+PG R
Sbjct: 110 KESPQ-FLQKISDPKLRAWAEQLHLLWKKLGKKIKPEVLSQPERFSLIYSQHPFIVPGGR 169

Query: 198 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 257
           F E YYWDSYW++ GLL S+M +T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 170 FVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVTAYGHIPNGGRVYYLQRSQPPLLT 229

Query: 258 SMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGN-HSLSRYYAMWNEP 317
            M+      TGDL F+R ++  L  E  FW     + T+  + GGN H+L+RY+  +  P
Sbjct: 230 LMMDRYVAHTGDLAFLRENIETLALELDFW---AENRTISVSSGGNSHTLNRYHVPYGGP 289

Query: 318 RPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWM---RDHTDLSTLATTSI 377
           RPES   D +LA         + L+ EL + AESGWDFSSRW+    +   L ++ T+ +
Sbjct: 290 RPESYSKDTELAHTLPEG-SWETLWAELKAGAESGWDFSSRWLVGSPNPDSLGSIRTSKL 349

Query: 378 LPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL 437
           +PVDLN F+ + E  +S     +G+   A K+      R   + ++ W+ +KG W DY L
Sbjct: 350 VPVDLNAFLCQAEELLSGFYSRLGNESQATKYRNLRAQRIAALTALLWDEDKGAWFDYDL 409

Query: 438 DNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNA-GIA 497
           +N           + +N   Y SN  PLW   F + +I   K L+ L+ S +L +  GI 
Sbjct: 410 EN-----------QKKNHEFYPSNLTPLWAGCFSDPAI-ADKALQYLQDSQILNHRHGIP 469

Query: 498 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 557
           TS  N+G+QWDFPN WAP+Q ++I GLA+S     + +A  +A  W+RTN+  Y +   M
Sbjct: 470 TSLQNTGQQWDFPNAWAPLQDLVIRGLAKSPSARTQEVAFQLAQNWIRTNFDVYSQRSAM 529

Query: 558 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
           +EKYD+      G GGEY  Q GFGW+NGV L  L+ +G
Sbjct: 530 YEKYDISNAQP-GGGGEYEVQEGFGWTNGVALMLLDRYG 550

BLAST of Cp4.1LG05g08040 vs. NCBI nr
Match: XP_023534407.1 (probable trehalase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1237 bits (3201), Expect = 0.0
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600

BLAST of Cp4.1LG05g08040 vs. NCBI nr
Match: XP_022958464.1 (probable trehalase [Cucurbita moschata])

HSP 1 Score: 1224 bits (3168), Expect = 0.0
Identity = 593/600 (98.83%), Postives = 596/600 (99.33%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTP VHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDP+DFVPRPQGFLPKVENA VRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARA GDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVH WDA++QNKNIYASNFIPLWIESFYNDSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600

BLAST of Cp4.1LG05g08040 vs. NCBI nr
Match: XP_022995786.1 (probable trehalase [Cucurbita maxima])

HSP 1 Score: 1208 bits (3125), Expect = 0.0
Identity = 584/600 (97.33%), Postives = 591/600 (98.50%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTPP HCSLTLFLFFLVV PLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPPCHCSLTLFLFFLVV-PLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLK+FI E
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKTFIGE 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDPVDFVPRPQGFLPKVENA VRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPC+IPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCVIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRN+LPALIKEH FWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNALPALIKEHTFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLY ELAS AESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYSELASTAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVH WDA++QNKNIYASNFIPLWIESFY+DSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYSDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATSTINSGEQWDFPNGWAPIQHMI+EGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RNSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIVEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 599

BLAST of Cp4.1LG05g08040 vs. NCBI nr
Match: KAG6605573.1 (Trehalase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1162 bits (3006), Expect = 0.0
Identity = 558/566 (98.59%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 35  MPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST 94
           MPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST
Sbjct: 1   MPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST 60

Query: 95  ERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVR 154
           ERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDP+DFVPRPQGFLPKVENA VR
Sbjct: 61  ERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVR 120

Query: 155 AWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLL 214
           AWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLL
Sbjct: 121 AWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLL 180

Query: 215 ASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVR 274
           ASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVR
Sbjct: 181 ASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVR 240

Query: 275 NSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNN 334
           NSLPALIKEH+FWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNN
Sbjct: 241 NSLPALIKEHVFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNN 300

Query: 335 YDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLAR 394
           YDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLAR
Sbjct: 301 YDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLAR 360

Query: 395 AVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIY 454
           A GDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVH WDA++QNKNIY
Sbjct: 361 AAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIY 420

Query: 455 ASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHM 514
           ASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHM
Sbjct: 421 ASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHM 480

Query: 515 IIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQT 574
           IIEGLARSELKEARVLAEDMAVRWLRTNYVAY+KTGYMHEKYDVQKCGDFGAGGEYVPQT
Sbjct: 481 IIEGLARSELKEARVLAEDMAVRWLRTNYVAYEKTGYMHEKYDVQKCGDFGAGGEYVPQT 540

Query: 575 GFGWSNGVVLAFLEEFGWPEDQNIDC 600
           GFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 GFGWSNGVVLAFLEEFGWPEDQNIDC 566

BLAST of Cp4.1LG05g08040 vs. NCBI nr
Match: XP_038907056.1 (probable trehalase [Benincasa hispida] >XP_038907058.1 probable trehalase [Benincasa hispida])

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 516/602 (85.71%), Postives = 554/602 (92.03%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQP   P     LTLF  FL++  L P  A +P  EV+SPRL  RGPV+PV+NLVKF
Sbjct: 1   MAFFQPHFPPIPFSPLTLFHLFLIMPLLRPATATLP--EVFSPRLVDRGPVVPVTNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDL+STE AFDAL RSSNGSV VE+LK+FI++
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLDSTETAFDALERSSNGSVSVENLKTFITD 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTD+VYS+PVDFVP+P GFLPKVEN+EVRAWAL+IHN+WKNLSR+VSDD+IHRP+
Sbjct: 121 YFDSAGTDLVYSEPVDFVPQPIGFLPKVENSEVRAWALDIHNFWKNLSRRVSDDLIHRPE 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
            HTLLPLP P ++PG+RFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 THTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGAR+YYTNRSQPPLLSSMVYDIYLRTGDLEFVRN+LPALIKEH FWNSGFHS+TVR AP
Sbjct: 241 NGARSYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNALPALIKEHKFWNSGFHSITVRNAP 300

Query: 301 GGN--HSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRW 360
           GGN  HSLSRYYAMWNEPRPESSLLDEKLASKFVNNY+KQ LYRE+ASAAESGWDFSSRW
Sbjct: 301 GGNGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKQHLYREIASAAESGWDFSSRW 360

Query: 361 MRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINS 420
           MRD TDLSTLATTSILPVDLNVFILKMELDISNLARAVGD+C A  F EASLVRKKTINS
Sbjct: 361 MRDSTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYCTAKHFFEASLVRKKTINS 420

Query: 421 IFWNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLK 480
           IFWNSEKGQWLDYWLDNG +KG H WDAR+QN+N+YASNFIPLWIESFY+DS QMKKVLK
Sbjct: 421 IFWNSEKGQWLDYWLDNGLYKGTHTWDARNQNQNVYASNFIPLWIESFYSDSRQMKKVLK 480

Query: 481 SLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRW 540
           SLR SGLLCNAGIATS INSGEQWDFPNGWAPIQHMI+EGLARS LKEAR LAED+A RW
Sbjct: 481 SLRNSGLLCNAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIAARW 540

Query: 541 LRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNI 600
           L+TNYVAYK TGYMHEKYDVQKCGDFG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQNI
Sbjct: 541 LQTNYVAYKHTGYMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQNI 600

BLAST of Cp4.1LG05g08040 vs. ExPASy TrEMBL
Match: A0A6J1H564 (Trehalase OS=Cucurbita moschata OX=3662 GN=LOC111459683 PE=3 SV=1)

HSP 1 Score: 1224 bits (3168), Expect = 0.0
Identity = 593/600 (98.83%), Postives = 596/600 (99.33%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTP VHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDP+DFVPRPQGFLPKVENA VRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARA GDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVH WDA++QNKNIYASNFIPLWIESFYNDSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600

BLAST of Cp4.1LG05g08040 vs. ExPASy TrEMBL
Match: A0A6J1K2W1 (Trehalase OS=Cucurbita maxima OX=3661 GN=LOC111491217 PE=3 SV=1)

HSP 1 Score: 1208 bits (3125), Expect = 0.0
Identity = 584/600 (97.33%), Postives = 591/600 (98.50%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTPP HCSLTLFLFFLVV PLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPPCHCSLTLFLFFLVV-PLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLK+FI E
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKTFIGE 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDPVDFVPRPQGFLPKVENA VRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPC+IPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCVIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRN+LPALIKEH FWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNALPALIKEHTFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLY ELAS AESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYSELASTAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVH WDA++QNKNIYASNFIPLWIESFY+DSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYSDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATSTINSGEQWDFPNGWAPIQHMI+EGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RNSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIVEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 599

BLAST of Cp4.1LG05g08040 vs. ExPASy TrEMBL
Match: A0A1S3ATK4 (Trehalase OS=Cucumis melo OX=3656 GN=LOC103482823 PE=3 SV=1)

HSP 1 Score: 1048 bits (2710), Expect = 0.0
Identity = 507/600 (84.50%), Postives = 545/600 (90.83%), Query Frame = 0

Query: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           M    P L P    SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKF
Sbjct: 1   MPVLHPHLPPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS 
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISN 120

Query: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YF SAGTD+VYS+PVDF P+P GFLPKVEN EVR WAL+IHN+WKNLSR+VSDD+IH PD
Sbjct: 121 YFHSAGTDLVYSNPVDFNPQPDGFLPKVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
            HTLLPLP P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 THTLLPLPEPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGAR+YYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHSVTV+   
Sbjct: 241 NGARSYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG- 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
            GNHSLSRYYAMWNEPRPESSL+DEKLASKF NNY+K+ LYRE+ASAAESGWDFSSRWMR
Sbjct: 301 NGNHSLSRYYAMWNEPRPESSLVDEKLASKFANNYEKKHLYREIASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420
           D TDLSTLATTSILPVDLNVFILKMELDISNLARAVGD+C A  F EASLVRKKTINSIF
Sbjct: 361 DSTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGS+KG H+W+AR+QN+NIYASNFIPLWIESFY+DS QMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSYKGAHSWNARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLR
Sbjct: 481 RNSGLLCNAGIATSVINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYK TG+MHEKYDVQKCGDFG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 541 TNYVAYKHTGFMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of Cp4.1LG05g08040 vs. ExPASy TrEMBL
Match: A0A0A0KMF2 (Trehalase OS=Cucumis sativus OX=3659 GN=Csa_5G149880 PE=3 SV=1)

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 500/592 (84.46%), Postives = 542/592 (91.55%), Query Frame = 0

Query: 10  PPVH-CSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVA 69
           PP+   SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKFLER+QVVA
Sbjct: 9   PPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKFLERIQVVA 68

Query: 70  LNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTD 129
           LNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS YFDSAGTD
Sbjct: 69  LNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTD 128

Query: 130 MVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLP 189
           +VYS+P DF P P GFLPKVEN EVRAWA +IHN+WKNLSR+VSDD+IH PD HTLLPLP
Sbjct: 129 LVYSNPADFDPHPDGFLPKVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLP 188

Query: 190 MPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYT 249
            P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYT
Sbjct: 189 EPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYT 248

Query: 250 NRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSR 309
           NRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHS+TV+    GNHSLSR
Sbjct: 249 NRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSITVQNG-NGNHSLSR 308

Query: 310 YYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTL 369
           YYAMWNEPRPESSL+DEK+ASKFVNNY+K+ LYRE+ASAAESGWDFSSRWMRD TDLSTL
Sbjct: 309 YYAMWNEPRPESSLVDEKVASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTL 368

Query: 370 ATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQW 429
           ATTSILPVDLNVFILKMELDISNLARAVGD+C A  F EASLVRKKTINSIFWNSEKGQW
Sbjct: 369 ATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQW 428

Query: 430 LDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCN 489
           LDYWLDNGS+KG H+WD R+QN+N+YASNFIPLW+ESFY+DS QMKKVLKSLR SGLLCN
Sbjct: 429 LDYWLDNGSYKGAHSWDVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCN 488

Query: 490 AGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKK 549
           AGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLRTNYVAYK 
Sbjct: 489 AGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKH 548

Query: 550 TGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TG+MHEKYDVQKCG FG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 549 TGFMHEKYDVQKCGGFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of Cp4.1LG05g08040 vs. ExPASy TrEMBL
Match: A0A5A7TH01 (Trehalase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001860 PE=3 SV=1)

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 497/574 (86.59%), Postives = 532/574 (92.68%), Query Frame = 0

Query: 27  PLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 86
           PLL PAA   L EV+SPRLA +GPVIPV NLVKFLERLQVVALNSFGKLDFDLKYYVDLS
Sbjct: 2   PLLRPAAAS-LPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 61

Query: 87  LKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPVDFVPRPQGFLP 146
           LKFDLNST+ AFDAL RSSNGSV VE+L++FIS YF SAGTD+VYS+PVDF P+P GFLP
Sbjct: 62  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 121

Query: 147 KVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDS 206
           KVEN EVR WAL+IHN+WKNLSR+VSDD+IH PD HTLLPLP P ++PG+RFRE+YYWDS
Sbjct: 122 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 181

Query: 207 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 266
           YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 182 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 241

Query: 267 TGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEK 326
           TGDLEFVRNSLPALIKEH FWNSGFHSVTV+    GNHSLSRYYAMWNEPRPESSL+DEK
Sbjct: 242 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG-NGNHSLSRYYAMWNEPRPESSLVDEK 301

Query: 327 LASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKME 386
           LASKF NNY+K+ LYRE+ASAAESGWDFSSRWMRD TDLSTLATTSILPVDLNVFILKME
Sbjct: 302 LASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKME 361

Query: 387 LDISNLARAVGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHAWDA 446
           LDISNLARAVGD+C A  F EASLVRKKTINSIFWNSEKGQWLDYWLDNGS+KG H+W+A
Sbjct: 362 LDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWNA 421

Query: 447 RSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPN 506
           R+QN+NIYASNFIPLWIESFY+DS QMKKVLKSLR SGLLCNAGIATS INSGEQWDFPN
Sbjct: 422 RNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDFPN 481

Query: 507 GWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGA 566
           GWAPIQHMI+EGLARSEL EA+ LAED+A RWLRTNYVAYK TG+MHEKYDVQKCGDFG 
Sbjct: 482 GWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDFGG 541

Query: 567 GGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 542 GGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 573

BLAST of Cp4.1LG05g08040 vs. TAIR 10
Match: AT4G24040.1 (trehalase 1 )

HSP 1 Score: 673.7 bits (1737), Expect = 1.4e-193
Identity = 317/555 (57.12%), Postives = 415/555 (74.77%), Query Frame = 0

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDF-DLKYYVDLSLK--FDLNSTERAFDAL-SRS 108
           GPV+  + LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E AFD L S S
Sbjct: 72  GPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTSES 131

Query: 109 SNGSVPVEDLKSFISEYFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYW 168
            +  VPVE L+ F+ EYFD AG D+++ +PVDFV  P GFL  VEN EVR WA E+H  W
Sbjct: 132 HDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHGLW 191

Query: 169 KNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKG 228
           +NLS +VSD +    D HTLLPLP P IIPG+RFREVYYWDSYW+I+GL+ S+M+ TAKG
Sbjct: 192 RNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTAKG 251

Query: 229 IVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH 288
           +V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E VR ++P L+KE+
Sbjct: 252 LVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLKEY 311

Query: 289 MFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYREL 348
            FWNSG H V +R A G +H LSRYYAMWN+PRPESS+ DE+ AS F    +KQR +R++
Sbjct: 312 EFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHRDI 371

Query: 349 ASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYK 408
           A+AAESG DFS+RWMRD  + +T+ATTS++PVDLNVF+LKMELDI+ + +  GD   + +
Sbjct: 372 ATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGSDR 431

Query: 409 FLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIE 468
           F++AS  R+K   ++FWN + GQWLDYWL +   +    W A +QN N++ASNF P+WI 
Sbjct: 432 FVKASKAREKAFQTVFWNEKAGQWLDYWLSSSGEES-ETWKAENQNTNVFASNFAPIWIN 491

Query: 469 SFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSEL 528
           S  +D   +KKV+ +L+ SGL+  AGI TS  NSG+QWD PNGWAP Q MI+ GL RS +
Sbjct: 492 SINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRSSV 551

Query: 529 KEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVL 588
           KEA+ +AED+A RW+++NY+ YKK+G +HEK  V + G++G GGEY+PQTGFGWSNGV+L
Sbjct: 552 KEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGVIL 611

Query: 589 AFLEEFGWPEDQNID 600
           AFLEE+GWP   +I+
Sbjct: 612 AFLEEYGWPSHLSIE 625