Cp4.1LG05g06840 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG05g06840 vs. ExPASy Swiss-Prot
Match: Q9C5X3 (SNARE-interacting protein KEULE OS=Arabidopsis thaliana OX=3702 GN=KEU PE=1 SV=2)

HSP 1 Score: 1005.0 bits (2597), Expect = 4.2e-292
Identity = 519/694 (74.78%), Postives = 601/694 (86.60%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MS+SDSDSSS  G++K+F+QI+RERLL+EMLRSAKTG SKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSYSDSDSSSHGGEYKNFRQITRERLLYEMLRSAKTGSSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADIT EGVSLVEDI+RRRQPLPSMDAIYF+QP++ENV+MFL+DMSG+SPLY+KAFVFF
Sbjct: 61  KMADITQEGVSLVEDIFRRRQPLPSMDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSP+S+ELV  IK+DS+VLPRI AL+EMNLE+FAIDSQGFIT+ E+ALE+LF D+E+S+K
Sbjct: 121 SSPVSKELVGHIKKDSSVLPRIGALREMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           G ACLN  A RI TVFASLR                         EFP+VRY+AAKSLDA
Sbjct: 181 GDACLNVMASRIATVFASLR-------------------------EFPAVRYRAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           +TMTT RDLIPTK+AAG+++ +  +K++I NFPQ+ETCELLILDRS+DQIAPV+HEWTYD
Sbjct: 241 STMTTLRDLIPTKLAAGIWNCLAKHKQSIENFPQTETCELLILDRSIDQIAPVIHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMC DLL+MEGNKYVH IPSK GG PEKK+VLLE+HDP+WLELRHAHIADAS RLH+KM 
Sbjct: 301 AMCHDLLNMEGNKYVHVIPSKSGGQPEKKDVLLEEHDPIWLELRHAHIADASERLHDKMT 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           +F+SKNKAAQ+ Q  R  +ELSTRDLQKMVQALPQYSEQI+KLSLHVEIA KLN +I+++
Sbjct: 361 NFLSKNKAAQL-QGKRDGAELSTRDLQKMVQALPQYSEQIDKLSLHVEIARKLNDLIREQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAG KDVIK+L+T E+ SRE KLRLLMILA IYPEKFEGEKG NL
Sbjct: 421 GLRELGQLEQDLVFGDAGMKDVIKYLSTQEEASREGKLRLLMILATIYPEKFEGEKGQNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKL  DDM AV NM LLG A  D+KK++ G F+LKFD+HKKKRAVRK+ + EE  WQ
Sbjct: 481 MKLAKLSSDDMTAVNNMSLLGSAV-DAKKNTPGGFTLKFDLHKKKRAVRKE-RQEEAAWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGP---SHTAAVQQLPSAHSMRSRR 600
           LSRFYP+IEEL+EKLSKGEL KED+PC+NDPS ++HG    S  A+  Q  +A SMRSRR
Sbjct: 541 LSRFYPMIEELIEKLSKGELPKEDFPCMNDPSPSFHGSTSLSSAASSSQGQAAQSMRSRR 600

Query: 601 TPTWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKRE 660
           TPTWA+PR SDDGYSSDSVLRHASSDF+KMGQRIFVFIVGGATRSEL+VCHKL++KLKRE
Sbjct: 601 TPTWAKPRGSDDGYSSDSVLRHASSDFRKMGQRIFVFIVGGATRSELKVCHKLSTKLKRE 660

Query: 661 VVLGSTSIDDPPQFITKLKMLTA-QELSLDDLQI 691
           V+LGSTS+DDPPQFITKLK+LTA  +LSLDDLQI
Sbjct: 661 VILGSTSLDDPPQFITKLKLLTANDDLSLDDLQI 666

BLAST of Cp4.1LG05g06840 vs. ExPASy Swiss-Prot
Match: Q9C5P7 (Protein transport Sec1a OS=Arabidopsis thaliana OX=3702 GN=SEC1A PE=2 SV=3)

HSP 1 Score: 872.1 bits (2252), Expect = 4.2e-252
Identity = 457/697 (65.57%), Postives = 554/697 (79.48%), Query Frame = 0

Query: 1   MSFSDSDSSSQ---CGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMS 60
           MSFSDS+SSS     GD+K F+QISR+RLLHEML S KTGDSK+ WK+LIMD++TVK+MS
Sbjct: 1   MSFSDSESSSHGGGGGDYKFFRQISRDRLLHEMLGSTKTGDSKA-WKILIMDRVTVKVMS 60

Query: 61  YACKMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAF 120
            +CKMADITD+G+SLVE++++RR+P+P MDAIYF+QPS+EN+VMFL+DMSGR PLYRKAF
Sbjct: 61  QSCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKENIVMFLSDMSGREPLYRKAF 120

Query: 121 VFFSSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDD-E 180
           +FFSS I +ELV  IK DS+VLPRI AL+EMN+EYF ID+QGF+T+ E+ALE L+ +D E
Sbjct: 121 IFFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPIDNQGFLTDHEQALETLYAEDAE 180

Query: 181 SSQKGAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAK 240
           +S+    CLN  A RI TVFASL+                         E P VRY+AAK
Sbjct: 181 NSRHFHICLNIMATRIATVFASLK-------------------------ELPFVRYRAAK 240

Query: 241 SLDATTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHE 300
           S      T  RDL+P+K+AA ++D I+ Y K IPNFPQ+ETCELLI+DRSVDQIAP++HE
Sbjct: 241 S------TASRDLVPSKLAAAIWDCISKY-KAIPNFPQTETCELLIVDRSVDQIAPIIHE 300

Query: 301 WTYDAMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLH 360
           WTYDAMC DLL MEGNK+V E+PSK GGPPEKKE++LEDHDPVWLELRH HIADAS RLH
Sbjct: 301 WTYDAMCHDLLDMEGNKHVIEVPSKTGGPPEKKEIVLEDHDPVWLELRHTHIADASERLH 360

Query: 361 EKMAHFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKI 420
           EKM +F SKNKAAQM   SR  SELSTRDLQK+VQALPQY EQ++KLS HVE+A K+N+I
Sbjct: 361 EKMTNFASKNKAAQMR--SRDGSELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRI 420

Query: 421 IKDEGLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEK 480
           I+D GLR+LGQLEQDLVFGDAG KDVI FL TN+D + ENKLRLLMI A +YPEKFEG+K
Sbjct: 421 IRDTGLRDLGQLEQDLVFGDAGAKDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDK 480

Query: 481 GLNLMKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEE 540
           G+ LM+LA+L P DM  ++NM+L+ G +P++K  S GSFSLKFD  K K+A RK   GEE
Sbjct: 481 GVKLMQLARLSPVDMKVISNMQLIAG-SPENKAKS-GSFSLKFDAGKTKQANRKDRSGEE 540

Query: 541 ETWQLSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQ--LPSA---- 600
           ETWQL RFYP+IEEL+EKL KG+LSK DY C+N  S      + T +V++   P+A    
Sbjct: 541 ETWQLFRFYPMIEELLEKLVKGDLSKSDYLCMNQSSHKEESEARTGSVRKSSAPTAVPER 600

Query: 601 ----HSMRSRRTPTWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRV 660
               HSMRSRRT TWARP +SDDGYSSDSVL+ AS++FKK+GQRIFVFI+GGATRSELRV
Sbjct: 601 KATPHSMRSRRTATWARPHSSDDGYSSDSVLKSASTEFKKLGQRIFVFIIGGATRSELRV 660

Query: 661 CHKLTSKLKREVVLGSTSIDDPPQFITKLKMLTAQEL 684
           CHKLTS L+REVVLGSTS DDPPQ+ITKLK+L+ +++
Sbjct: 661 CHKLTSSLRREVVLGSTSFDDPPQYITKLKLLSEKDI 660

BLAST of Cp4.1LG05g06840 vs. ExPASy Swiss-Prot
Match: Q9SZ77 (Protein transport Sec1b OS=Arabidopsis thaliana OX=3702 GN=SEC1B PE=2 SV=3)

HSP 1 Score: 859.8 bits (2220), Expect = 2.2e-248
Identity = 447/692 (64.60%), Postives = 537/692 (77.60%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDS SSS  G++K+F+QI+RERLL EMLR  + G SK TWKVL+MDK TVKIMS AC
Sbjct: 1   MSFSDSGSSSYGGEYKNFRQITRERLLCEMLRPERNGSSKLTWKVLVMDKFTVKIMSSAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KM++IT EG+SLVE I + RQP+ +M+ IYF+QP+ ENV  FL+DM+G+SPLY+KAFVFF
Sbjct: 61  KMSEITQEGISLVEVITKHRQPMTAMEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSP+SR LV  IK+D   + RI  LKEMNLEY ++D QGF+TN+E ALEELFCDDE+ Q+
Sbjct: 121 SSPVSRSLVNLIKKDMRAMKRIGGLKEMNLEYISMDIQGFVTNNENALEELFCDDENHQR 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
             ACLN  A RI TV ASL+                         E+P VRY+ AK+LDA
Sbjct: 181 ADACLNVVAKRIATVLASLK-------------------------EYPFVRYRGAKALDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTT+R+LIPTK+AA V++ +  YK+TI +FPQ+ETCELLILDRS+DQIAP++HEWTYD
Sbjct: 241 TTMTTYRELIPTKLAASVWNCLARYKQTIEDFPQTETCELLILDRSIDQIAPLIHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMC DLL+MEGNKY HE+PSK G  PEKKEVLL++ D +W+ELR AHIADAS RLHEKM 
Sbjct: 301 AMCHDLLNMEGNKYTHEVPSKTGDKPEKKEVLLDEEDSIWVELRDAHIADASERLHEKMT 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           +FVSKNKAAQ+   S+   +LS++DLQKMV ALPQYSEQI+KLSLHVEIA  +N+ I ++
Sbjct: 361 NFVSKNKAAQLKHSSKDFGDLSSKDLQKMVHALPQYSEQIDKLSLHVEIARTINRTIMEQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLR+LGQLEQDLVFGDAG KDVIKFL+TN  +S E+KLRL+MI+AAIYP+KFEGEKG  +
Sbjct: 421 GLRDLGQLEQDLVFGDAGRKDVIKFLSTNHIISHESKLRLIMIVAAIYPKKFEGEKGRKM 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           M+LAKL  DD+ AV NMRLLG    + KKS+ GSF LKFD+ K KRA R+   GE +TWQ
Sbjct: 481 MELAKLSGDDVVAVNNMRLLGPVHTECKKSTTGSFPLKFDVLKTKRAARRDRVGETQTWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQT-YHGPSHTAAVQQLPSAHSMRSRRTP 600
           LSRFYPI+EELVEKLSKG L K+DYPC+N+P  T Y G    +A   LP      SRRTP
Sbjct: 541 LSRFYPIVEELVEKLSKGHLPKQDYPCMNEPKPTFYSGSLSPSASPVLP-----HSRRTP 600

Query: 601 TWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVV 660
           TWAR   SDDGY SDSVL  ASS FK+ GQRIFVFIVGGATRSELRVCHKLT KL REV+
Sbjct: 601 TWARRHLSDDGYFSDSVLGRASSGFKRKGQRIFVFIVGGATRSELRVCHKLTEKLDREVI 660

Query: 661 LGSTSIDDPPQFITKLKMLT-AQELSLDDLQI 691
           LGS+S  DP  F+TK+K L   +E+SLDD+ I
Sbjct: 661 LGSSSFLDPLTFLTKMKQLNEEEEISLDDIVI 662

BLAST of Cp4.1LG05g06840 vs. ExPASy Swiss-Prot
Match: Q7XWP3 (Probable protein transport Sec1a OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0252400 PE=3 SV=2)

HSP 1 Score: 851.7 bits (2199), Expect = 5.9e-246
Identity = 447/696 (64.22%), Postives = 549/696 (78.88%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MS     SS    D++SF+QI+R+RLL EMLRS K   SKS WKVLIMDKLTVKIMS++C
Sbjct: 1   MSMDSVISSRSGADYRSFRQITRDRLLFEMLRSTKK-SSKSAWKVLIMDKLTVKIMSFSC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMAD+ +EGVSLVED+Y RRQPLP MDAIYF+QP++EN+ +F++DMSG+ PLY+KA+VFF
Sbjct: 61  KMADVMEEGVSLVEDLYMRRQPLPLMDAIYFIQPTKENIRIFMSDMSGKIPLYKKAYVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDD-ESSQ 180
           SSP+ RELV QIK+DS V  RI AL EMNLEYFAIDSQGF T+ +KALEELF ++ E S 
Sbjct: 121 SSPVQRELVAQIKKDSNVRARIGALSEMNLEYFAIDSQGFTTDHDKALEELFSENAEGSL 180

Query: 181 KGAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLD 240
           K  +CLN  A RI TVFAS+R                         EFP V Y+ A+++D
Sbjct: 181 KYNSCLNMMATRIATVFASMR-------------------------EFPRVHYRVARTID 240

Query: 241 ATTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTY 300
           A+T+TT RDL PTK+AAGV++ +  +K  IP FPQ+ETCELLI+DRS+DQIAP++HEWTY
Sbjct: 241 ASTLTTLRDLAPTKLAAGVWNCLARFKAMIPEFPQTETCELLIVDRSIDQIAPIIHEWTY 300

Query: 301 DAMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKM 360
           DAMC DLL M+GNKYV ++PSK G   E KEVLLEDHDP+WLELRH HIA+AS RLHEKM
Sbjct: 301 DAMCHDLLCMDGNKYVQQVPSKSGSGTENKEVLLEDHDPIWLELRHVHIANASERLHEKM 360

Query: 361 AHFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKD 420
            +FVSKNKAAQ+HQ +R+  +LST++LQKMVQALPQYS+QI+KL+LHVEIA KLN  IK+
Sbjct: 361 TNFVSKNKAAQLHQ-ARNGGDLSTKELQKMVQALPQYSDQIDKLALHVEIAGKLNSTIKE 420

Query: 421 EGLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLN 480
           + L+++GQLEQDLVFGDAGTK++I F  T+ D+SRENKLRLLM+ AAI P+K   +KG  
Sbjct: 421 QQLKDVGQLEQDLVFGDAGTKELINFFRTHLDISRENKLRLLMVYAAINPDKTRSDKGAK 480

Query: 481 LMKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETW 540
           LM+LA L  DDM AV+NMR L G   DSKKSS G F+LKFD+ KK+  +RK+  GEE  W
Sbjct: 481 LMQLAGLSADDMIAVSNMRCLCG--HDSKKSSAGGFTLKFDLRKKRHGIRKERIGEESKW 540

Query: 541 QLSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHG-PSHTAAVQQLPS---AHSMRS 600
            LSRFYPI+EEL+EKLSKGEL K++Y  LNDPS ++ G PS  A+ Q  P+   A SMRS
Sbjct: 541 MLSRFYPILEELIEKLSKGELPKDEYHYLNDPSPSFRGIPS--ASTQTSPAHQPAQSMRS 600

Query: 601 RRT-PTWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKL 660
           RRT  TWARPR+SDDGYSSDSVL+H SS+ +K+GQR+FVF++GGATRSEL   HKL+SKL
Sbjct: 601 RRTGGTWARPRDSDDGYSSDSVLKHTSSNSRKLGQRLFVFVIGGATRSELCAAHKLSSKL 660

Query: 661 KREVVLGSTSIDDPPQFITKLKMLTAQELSLDDLQI 691
           KRE++LGS+S+DDPPQFITKLKML+  +L+LDDLQI
Sbjct: 661 KREIILGSSSLDDPPQFITKLKMLSTDDLTLDDLQI 665

BLAST of Cp4.1LG05g06840 vs. ExPASy Swiss-Prot
Match: Q5VNU3 (Probable protein transport Sec1b OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0135900 PE=2 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 4.3e-228
Identity = 409/684 (59.80%), Postives = 519/684 (75.88%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MS S SD  + C D K F+ I R+R+L ++L+  K  ++KS+WKVLIMDK TV+IM+YAC
Sbjct: 1   MSMSGSDFGAPCDDPKIFRNICRDRILKDLLQPDKDKETKSSWKVLIMDKFTVRIMAYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMA+ITD G+SLVED+++RR+P+PSMDAIYFLQP +ENV+M L+DMSGR PLYRKA++FF
Sbjct: 61  KMAEITDAGISLVEDLFKRREPMPSMDAIYFLQPLKENVIMLLSDMSGRCPLYRKAYIFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPI +ELV+ IK DS+V+PRI AL+EMNLE+FAID QGF T+ + A  +L+    +S+K
Sbjct: 121 SSPIPKELVSYIKNDSSVIPRIGALREMNLEFFAIDMQGFTTDHDMAFTDLYSAQHNSKK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
               ++  A RI T FASL+                         EFP VRY+A K  D 
Sbjct: 181 FNDTISTMATRIATTFASLK-------------------------EFPCVRYRAPKGTDP 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
            T   F D++P  +A  V+D ++ YK TIP FPQ ETCELLI+DR +DQIAPV+HEWTYD
Sbjct: 241 MTTPKF-DMVPKWLATAVWDIVSKYKSTIPEFPQKETCELLIVDRPIDQIAPVIHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMC DLL M+G KY++E+ SK G  PE+KE LLEDHDP+W+ELRH HIADAS RL++KM 
Sbjct: 301 AMCHDLLEMDGQKYIYEV-SKAGSEPERKEALLEDHDPLWVELRHIHIADASERLYDKMN 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           +FVSKNKAAQ+H  SR   E+ST+DLQK+VQALPQY EQ+EKL+LH+EIA K+NK I++ 
Sbjct: 361 NFVSKNKAAQLH--SRDGGEISTKDLQKIVQALPQYGEQVEKLTLHIEIAGKINKFIREY 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLR++GQ+EQDLVFGDA  K+VI  L + +D+S ENKLRLL+I A +YPEKFEG+KG  L
Sbjct: 421 GLRDIGQVEQDLVFGDAAAKEVISILRSKQDMSPENKLRLLIIYAIVYPEKFEGDKGEKL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSL-GSFSLKFDIHKKKRAVRKQLKGEEETW 540
           M+LAKLP D+M+A+ ++R L G+  D+KK+S  G FSLKFD  KKK A R + +  +ETW
Sbjct: 481 MQLAKLPHDEMDAINSLRYLVGS--DTKKASRPGGFSLKFDAQKKKNAARTERQDGDETW 540

Query: 541 QLSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSM--RSRR 600
            LSRF+P+IEEL+EKLSKG L   +YP +++PS T  G + +AA  +   A  M  RSRR
Sbjct: 541 ALSRFFPLIEELIEKLSKGALPLNEYPSMSEPSSTEQGSTQSAAATKPAQAQPMSRRSRR 600

Query: 601 TPTWARPRNSDDGYSSD-SVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKR 660
           TPTWA+ RNSDD  SSD SVLRH S+DFK++G RIFVF++GGATRSELR  HKLT KLKR
Sbjct: 601 TPTWAKSRNSDDSQSSDSSVLRHGSNDFKRLGNRIFVFMIGGATRSELRTVHKLTMKLKR 653

Query: 661 EVVLGSTSIDDPPQFITKLKMLTA 681
           E+VLGS+SIDDPPQFI+KLKMLTA
Sbjct: 661 EIVLGSSSIDDPPQFISKLKMLTA 653

BLAST of Cp4.1LG05g06840 vs. NCBI nr
Match: XP_023533702.1 (SNARE-interacting protein KEULE-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1286 bits (3329), Expect = 0.0
Identity = 665/690 (96.38%), Postives = 665/690 (96.38%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIRIGTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRIGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 665

BLAST of Cp4.1LG05g06840 vs. NCBI nr
Match: XP_023533703.1 (SNARE-interacting protein KEULE-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1280 bits (3313), Expect = 0.0
Identity = 664/690 (96.23%), Postives = 664/690 (96.23%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIRIGTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRIGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK RAVRKQLKGEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK-RAVRKQLKGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 664

BLAST of Cp4.1LG05g06840 vs. NCBI nr
Match: XP_022995238.1 (SNARE-interacting protein KEULE-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1278 bits (3308), Expect = 0.0
Identity = 659/690 (95.51%), Postives = 663/690 (96.09%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIRIGTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRIGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKD+
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFE EKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFESEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQL GEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLNGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 665

BLAST of Cp4.1LG05g06840 vs. NCBI nr
Match: XP_022957838.1 (SNARE-interacting protein KEULE-like isoform X1 [Cucurbita moschata] >KAG6605695.1 SNARE-interacting protein KEULE, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1278 bits (3307), Expect = 0.0
Identity = 659/690 (95.51%), Postives = 664/690 (96.23%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDI+RRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIHRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIR+GTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRVGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYD ITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDCITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKDE
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDE 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 665

BLAST of Cp4.1LG05g06840 vs. NCBI nr
Match: XP_022995239.1 (SNARE-interacting protein KEULE-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 658/690 (95.36%), Postives = 662/690 (95.94%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIRIGTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRIGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKD+
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFE EKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFESEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK RAVRKQL GEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK-RAVRKQLNGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 664

BLAST of Cp4.1LG05g06840 vs. ExPASy TrEMBL
Match: A0A6J1K7B5 (SNARE-interacting protein KEULE-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490845 PE=3 SV=1)

HSP 1 Score: 1278 bits (3308), Expect = 0.0
Identity = 659/690 (95.51%), Postives = 663/690 (96.09%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIRIGTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRIGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKD+
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFE EKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFESEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQL GEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLNGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 665

BLAST of Cp4.1LG05g06840 vs. ExPASy TrEMBL
Match: A0A6J1H1E2 (SNARE-interacting protein KEULE-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459259 PE=3 SV=1)

HSP 1 Score: 1278 bits (3307), Expect = 0.0
Identity = 659/690 (95.51%), Postives = 664/690 (96.23%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDI+RRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIHRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIR+GTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRVGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYD ITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDCITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKDE
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDE 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 665

BLAST of Cp4.1LG05g06840 vs. ExPASy TrEMBL
Match: A0A6J1K556 (SNARE-interacting protein KEULE-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490845 PE=3 SV=1)

HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 658/690 (95.36%), Postives = 662/690 (95.94%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIRIGTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRIGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKD+
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFE EKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFESEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK RAVRKQL GEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK-RAVRKQLNGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 664

BLAST of Cp4.1LG05g06840 vs. ExPASy TrEMBL
Match: A0A6J1H357 (SNARE-interacting protein KEULE-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459259 PE=3 SV=1)

HSP 1 Score: 1272 bits (3291), Expect = 0.0
Identity = 658/690 (95.36%), Postives = 663/690 (96.09%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDI+RRRQPLPSMDAIYFLQPSRENVVMFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIHRRRQPLPSMDAIYFLQPSRENVVMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK
Sbjct: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           GAACLNETAIR+GTVFASLR                         EFPSVRYQAAKSLDA
Sbjct: 181 GAACLNETAIRVGTVFASLR-------------------------EFPSVRYQAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYD ITNYKKTIPNFPQSETCELLILDRS+DQIAPVMHEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDCITNYKKTIPNFPQSETCELLILDRSIDQIAPVMHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA
Sbjct: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNKIIKDE
Sbjct: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKIIKDE 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL
Sbjct: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK RAVRKQLKGEEETWQ
Sbjct: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKK-RAVRKQLKGEEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT
Sbjct: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTAQELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 664

BLAST of Cp4.1LG05g06840 vs. ExPASy TrEMBL
Match: A0A0A0LKG6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G150510 PE=3 SV=1)

HSP 1 Score: 1193 bits (3086), Expect = 0.0
Identity = 612/690 (88.70%), Postives = 642/690 (93.04%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDSDSSS  GD+KSF+QISR+RLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSY+C
Sbjct: 1   MSFSDSDSSSYGGDYKSFRQISRDRLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYSC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADITDEGVSLVEDIYRRRQPLPSMDAIYF+QPSRENV+MFL+DMSGRSPLYRKAFVFF
Sbjct: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFIQPSRENVIMFLSDMSGRSPLYRKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSPIS+ELV+QIKRDSTVLPRIAALKEMNLEYFAIDSQGF TN+EKALEELFCDDESSQK
Sbjct: 121 SSPISKELVSQIKRDSTVLPRIAALKEMNLEYFAIDSQGFTTNNEKALEELFCDDESSQK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           G ACLNE AIR+GTVFASLR                         EFP VRY+AAKSLDA
Sbjct: 181 GVACLNEMAIRVGTVFASLR-------------------------EFPFVRYRAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTTFRDLIPTKVAAGVYD IT YKKTIP+FPQSETCELLILDRS+DQIAPV+HEWTYD
Sbjct: 241 TTMTTFRDLIPTKVAAGVYDCITKYKKTIPDFPQSETCELLILDRSIDQIAPVIHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMCRDLLSMEGNKYVHE+PSKVGGPPEKKEVLLEDHDPVWLELRHAHIADAS RLHEKM 
Sbjct: 301 AMCRDLLSMEGNKYVHEVPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASERLHEKMT 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           +FVSKNKAAQ+HQ SR++SELSTRDLQKMVQALPQYSEQI+KLSLHVEIAVKLNK IK++
Sbjct: 361 NFVSKNKAAQIHQGSRNSSELSTRDLQKMVQALPQYSEQIDKLSLHVEIAVKLNKFIKEQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQ+EQDLVFGDAGTKDVIKFLTTNED SRENKLRLLMILAAIYPEKFEGEKG NL
Sbjct: 421 GLRELGQIEQDLVFGDAGTKDVIKFLTTNEDASRENKLRLLMILAAIYPEKFEGEKGQNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKLPP+DMNAVTNMRLLG  APDSKKSSLGSFSLKFDIHKKKRAVRKQ  G EETWQ
Sbjct: 481 MKLAKLPPEDMNAVTNMRLLG-TAPDSKKSSLGSFSLKFDIHKKKRAVRKQQNGGEETWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQLPSAHSMRSRRTPT 600
           LSRFYP+IEELVEKLSKGELSK+DYPCLNDPS TYHGPSHTAAVQ  P+AHSMRSRRTPT
Sbjct: 541 LSRFYPMIEELVEKLSKGELSKDDYPCLNDPSPTYHGPSHTAAVQPPPAAHSMRSRRTPT 600

Query: 601 WARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVVL 660
           WARPRNSDDGYSSDS+LRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLT+KLKREVVL
Sbjct: 601 WARPRNSDDGYSSDSILRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTAKLKREVVL 660

Query: 661 GSTSIDDPPQFITKLKMLTAQELSLDDLQI 690
           GSTSIDDPPQFITKLKMLTA ELSLDDLQI
Sbjct: 661 GSTSIDDPPQFITKLKMLTAHELSLDDLQI 664

BLAST of Cp4.1LG05g06840 vs. TAIR 10
Match: AT1G12360.1 (Sec1/munc18-like (SM) proteins superfamily )

HSP 1 Score: 1005.0 bits (2597), Expect = 3.0e-293
Identity = 519/694 (74.78%), Postives = 601/694 (86.60%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MS+SDSDSSS  G++K+F+QI+RERLL+EMLRSAKTG SKSTWKVLIMDKLTVKIMSYAC
Sbjct: 1   MSYSDSDSSSHGGEYKNFRQITRERLLYEMLRSAKTGSSKSTWKVLIMDKLTVKIMSYAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KMADIT EGVSLVEDI+RRRQPLPSMDAIYF+QP++ENV+MFL+DMSG+SPLY+KAFVFF
Sbjct: 61  KMADITQEGVSLVEDIFRRRQPLPSMDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSP+S+ELV  IK+DS+VLPRI AL+EMNLE+FAIDSQGFIT+ E+ALE+LF D+E+S+K
Sbjct: 121 SSPVSKELVGHIKKDSSVLPRIGALREMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
           G ACLN  A RI TVFASLR                         EFP+VRY+AAKSLDA
Sbjct: 181 GDACLNVMASRIATVFASLR-------------------------EFPAVRYRAAKSLDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           +TMTT RDLIPTK+AAG+++ +  +K++I NFPQ+ETCELLILDRS+DQIAPV+HEWTYD
Sbjct: 241 STMTTLRDLIPTKLAAGIWNCLAKHKQSIENFPQTETCELLILDRSIDQIAPVIHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMC DLL+MEGNKYVH IPSK GG PEKK+VLLE+HDP+WLELRHAHIADAS RLH+KM 
Sbjct: 301 AMCHDLLNMEGNKYVHVIPSKSGGQPEKKDVLLEEHDPIWLELRHAHIADASERLHDKMT 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           +F+SKNKAAQ+ Q  R  +ELSTRDLQKMVQALPQYSEQI+KLSLHVEIA KLN +I+++
Sbjct: 361 NFLSKNKAAQL-QGKRDGAELSTRDLQKMVQALPQYSEQIDKLSLHVEIARKLNDLIREQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLRELGQLEQDLVFGDAG KDVIK+L+T E+ SRE KLRLLMILA IYPEKFEGEKG NL
Sbjct: 421 GLRELGQLEQDLVFGDAGMKDVIKYLSTQEEASREGKLRLLMILATIYPEKFEGEKGQNL 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           MKLAKL  DDM AV NM LLG A  D+KK++ G F+LKFD+HKKKRAVRK+ + EE  WQ
Sbjct: 481 MKLAKLSSDDMTAVNNMSLLGSAV-DAKKNTPGGFTLKFDLHKKKRAVRKE-RQEEAAWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGP---SHTAAVQQLPSAHSMRSRR 600
           LSRFYP+IEEL+EKLSKGEL KED+PC+NDPS ++HG    S  A+  Q  +A SMRSRR
Sbjct: 541 LSRFYPMIEELIEKLSKGELPKEDFPCMNDPSPSFHGSTSLSSAASSSQGQAAQSMRSRR 600

Query: 601 TPTWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKRE 660
           TPTWA+PR SDDGYSSDSVLRHASSDF+KMGQRIFVFIVGGATRSEL+VCHKL++KLKRE
Sbjct: 601 TPTWAKPRGSDDGYSSDSVLRHASSDFRKMGQRIFVFIVGGATRSELKVCHKLSTKLKRE 660

Query: 661 VVLGSTSIDDPPQFITKLKMLTA-QELSLDDLQI 691
           V+LGSTS+DDPPQFITKLK+LTA  +LSLDDLQI
Sbjct: 661 VILGSTSLDDPPQFITKLKLLTANDDLSLDDLQI 666

BLAST of Cp4.1LG05g06840 vs. TAIR 10
Match: AT1G02010.1 (secretory 1A )

HSP 1 Score: 872.1 bits (2252), Expect = 3.0e-253
Identity = 457/697 (65.57%), Postives = 554/697 (79.48%), Query Frame = 0

Query: 1   MSFSDSDSSSQ---CGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMS 60
           MSFSDS+SSS     GD+K F+QISR+RLLHEML S KTGDSK+ WK+LIMD++TVK+MS
Sbjct: 1   MSFSDSESSSHGGGGGDYKFFRQISRDRLLHEMLGSTKTGDSKA-WKILIMDRVTVKVMS 60

Query: 61  YACKMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAF 120
            +CKMADITD+G+SLVE++++RR+P+P MDAIYF+QPS+EN+VMFL+DMSGR PLYRKAF
Sbjct: 61  QSCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKENIVMFLSDMSGREPLYRKAF 120

Query: 121 VFFSSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDD-E 180
           +FFSS I +ELV  IK DS+VLPRI AL+EMN+EYF ID+QGF+T+ E+ALE L+ +D E
Sbjct: 121 IFFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPIDNQGFLTDHEQALETLYAEDAE 180

Query: 181 SSQKGAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAK 240
           +S+    CLN  A RI TVFASL+                         E P VRY+AAK
Sbjct: 181 NSRHFHICLNIMATRIATVFASLK-------------------------ELPFVRYRAAK 240

Query: 241 SLDATTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHE 300
           S      T  RDL+P+K+AA ++D I+ Y K IPNFPQ+ETCELLI+DRSVDQIAP++HE
Sbjct: 241 S------TASRDLVPSKLAAAIWDCISKY-KAIPNFPQTETCELLIVDRSVDQIAPIIHE 300

Query: 301 WTYDAMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLH 360
           WTYDAMC DLL MEGNK+V E+PSK GGPPEKKE++LEDHDPVWLELRH HIADAS RLH
Sbjct: 301 WTYDAMCHDLLDMEGNKHVIEVPSKTGGPPEKKEIVLEDHDPVWLELRHTHIADASERLH 360

Query: 361 EKMAHFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKI 420
           EKM +F SKNKAAQM   SR  SELSTRDLQK+VQALPQY EQ++KLS HVE+A K+N+I
Sbjct: 361 EKMTNFASKNKAAQMR--SRDGSELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRI 420

Query: 421 IKDEGLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEK 480
           I+D GLR+LGQLEQDLVFGDAG KDVI FL TN+D + ENKLRLLMI A +YPEKFEG+K
Sbjct: 421 IRDTGLRDLGQLEQDLVFGDAGAKDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDK 480

Query: 481 GLNLMKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEE 540
           G+ LM+LA+L P DM  ++NM+L+ G +P++K  S GSFSLKFD  K K+A RK   GEE
Sbjct: 481 GVKLMQLARLSPVDMKVISNMQLIAG-SPENKAKS-GSFSLKFDAGKTKQANRKDRSGEE 540

Query: 541 ETWQLSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQ--LPSA---- 600
           ETWQL RFYP+IEEL+EKL KG+LSK DY C+N  S      + T +V++   P+A    
Sbjct: 541 ETWQLFRFYPMIEELLEKLVKGDLSKSDYLCMNQSSHKEESEARTGSVRKSSAPTAVPER 600

Query: 601 ----HSMRSRRTPTWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRV 660
               HSMRSRRT TWARP +SDDGYSSDSVL+ AS++FKK+GQRIFVFI+GGATRSELRV
Sbjct: 601 KATPHSMRSRRTATWARPHSSDDGYSSDSVLKSASTEFKKLGQRIFVFIIGGATRSELRV 660

Query: 661 CHKLTSKLKREVVLGSTSIDDPPQFITKLKMLTAQEL 684
           CHKLTS L+REVVLGSTS DDPPQ+ITKLK+L+ +++
Sbjct: 661 CHKLTSSLRREVVLGSTSFDDPPQYITKLKLLSEKDI 660

BLAST of Cp4.1LG05g06840 vs. TAIR 10
Match: AT4G12120.1 (Sec1/munc18-like (SM) proteins superfamily )

HSP 1 Score: 859.8 bits (2220), Expect = 1.5e-249
Identity = 447/692 (64.60%), Postives = 537/692 (77.60%), Query Frame = 0

Query: 1   MSFSDSDSSSQCGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMSYAC 60
           MSFSDS SSS  G++K+F+QI+RERLL EMLR  + G SK TWKVL+MDK TVKIMS AC
Sbjct: 1   MSFSDSGSSSYGGEYKNFRQITRERLLCEMLRPERNGSSKLTWKVLVMDKFTVKIMSSAC 60

Query: 61  KMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAFVFF 120
           KM++IT EG+SLVE I + RQP+ +M+ IYF+QP+ ENV  FL+DM+G+SPLY+KAFVFF
Sbjct: 61  KMSEITQEGISLVEVITKHRQPMTAMEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFF 120

Query: 121 SSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDDESSQK 180
           SSP+SR LV  IK+D   + RI  LKEMNLEY ++D QGF+TN+E ALEELFCDDE+ Q+
Sbjct: 121 SSPVSRSLVNLIKKDMRAMKRIGGLKEMNLEYISMDIQGFVTNNENALEELFCDDENHQR 180

Query: 181 GAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAKSLDA 240
             ACLN  A RI TV ASL+                         E+P VRY+ AK+LDA
Sbjct: 181 ADACLNVVAKRIATVLASLK-------------------------EYPFVRYRGAKALDA 240

Query: 241 TTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHEWTYD 300
           TTMTT+R+LIPTK+AA V++ +  YK+TI +FPQ+ETCELLILDRS+DQIAP++HEWTYD
Sbjct: 241 TTMTTYRELIPTKLAASVWNCLARYKQTIEDFPQTETCELLILDRSIDQIAPLIHEWTYD 300

Query: 301 AMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLHEKMA 360
           AMC DLL+MEGNKY HE+PSK G  PEKKEVLL++ D +W+ELR AHIADAS RLHEKM 
Sbjct: 301 AMCHDLLNMEGNKYTHEVPSKTGDKPEKKEVLLDEEDSIWVELRDAHIADASERLHEKMT 360

Query: 361 HFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKIIKDE 420
           +FVSKNKAAQ+   S+   +LS++DLQKMV ALPQYSEQI+KLSLHVEIA  +N+ I ++
Sbjct: 361 NFVSKNKAAQLKHSSKDFGDLSSKDLQKMVHALPQYSEQIDKLSLHVEIARTINRTIMEQ 420

Query: 421 GLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEKGLNL 480
           GLR+LGQLEQDLVFGDAG KDVIKFL+TN  +S E+KLRL+MI+AAIYP+KFEGEKG  +
Sbjct: 421 GLRDLGQLEQDLVFGDAGRKDVIKFLSTNHIISHESKLRLIMIVAAIYPKKFEGEKGRKM 480

Query: 481 MKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEEETWQ 540
           M+LAKL  DD+ AV NMRLLG    + KKS+ GSF LKFD+ K KRA R+   GE +TWQ
Sbjct: 481 MELAKLSGDDVVAVNNMRLLGPVHTECKKSTTGSFPLKFDVLKTKRAARRDRVGETQTWQ 540

Query: 541 LSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQT-YHGPSHTAAVQQLPSAHSMRSRRTP 600
           LSRFYPI+EELVEKLSKG L K+DYPC+N+P  T Y G    +A   LP      SRRTP
Sbjct: 541 LSRFYPIVEELVEKLSKGHLPKQDYPCMNEPKPTFYSGSLSPSASPVLP-----HSRRTP 600

Query: 601 TWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRVCHKLTSKLKREVV 660
           TWAR   SDDGY SDSVL  ASS FK+ GQRIFVFIVGGATRSELRVCHKLT KL REV+
Sbjct: 601 TWARRHLSDDGYFSDSVLGRASSGFKRKGQRIFVFIVGGATRSELRVCHKLTEKLDREVI 660

Query: 661 LGSTSIDDPPQFITKLKMLT-AQELSLDDLQI 691
           LGS+S  DP  F+TK+K L   +E+SLDD+ I
Sbjct: 661 LGSSSFLDPLTFLTKMKQLNEEEEISLDDIVI 662

BLAST of Cp4.1LG05g06840 vs. TAIR 10
Match: AT1G02010.2 (secretory 1A )

HSP 1 Score: 840.1 bits (2169), Expect = 1.3e-243
Identity = 445/697 (63.85%), Postives = 542/697 (77.76%), Query Frame = 0

Query: 1   MSFSDSDSSSQ---CGDHKSFKQISRERLLHEMLRSAKTGDSKSTWKVLIMDKLTVKIMS 60
           MSFSDS+SSS     GD+K F+QISR+RLLHEML S KTGDSK+ WK+LIMD++TVK+MS
Sbjct: 1   MSFSDSESSSHGGGGGDYKFFRQISRDRLLHEMLGSTKTGDSKA-WKILIMDRVTVKVMS 60

Query: 61  YACKMADITDEGVSLVEDIYRRRQPLPSMDAIYFLQPSRENVVMFLADMSGRSPLYRKAF 120
            +CKMADITD+G+SLVE++++RR+P+P MDAIYF+QPS+EN+VMFL+DMSGR PLYRKAF
Sbjct: 61  QSCKMADITDQGISLVEELFKRREPMPGMDAIYFIQPSKENIVMFLSDMSGREPLYRKAF 120

Query: 121 VFFSSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDSQGFITNSEKALEELFCDD-E 180
           +FFSS I +ELV  IK DS+VLPRI AL+EMN+EYF ID+QGF+T+ E+ALE L+ +D E
Sbjct: 121 IFFSSTIPKELVNHIKSDSSVLPRIGALREMNMEYFPIDNQGFLTDHEQALETLYAEDAE 180

Query: 181 SSQKGAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSDLPNLFLLQEFPSVRYQAAK 240
           +S+    CLN  A RI TVFASL+                         E P VRY+AAK
Sbjct: 181 NSRHFHICLNIMATRIATVFASLK-------------------------ELPFVRYRAAK 240

Query: 241 SLDATTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSETCELLILDRSVDQIAPVMHE 300
           S      T  RDL+P+K+AA ++D I+ Y K IPNFPQ+ETCELLI+DRSVDQIAP++HE
Sbjct: 241 S------TASRDLVPSKLAAAIWDCISKY-KAIPNFPQTETCELLIVDRSVDQIAPIIHE 300

Query: 301 WTYDAMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDHDPVWLELRHAHIADASVRLH 360
           WTYDAMC DLL MEGNK+V E+PSK GGPPEKKE++LEDHDPVWLELRH HIADAS RLH
Sbjct: 301 WTYDAMCHDLLDMEGNKHVIEVPSKTGGPPEKKEIVLEDHDPVWLELRHTHIADASERLH 360

Query: 361 EKMAHFVSKNKAAQMHQVSRSTSELSTRDLQKMVQALPQYSEQIEKLSLHVEIAVKLNKI 420
           EKM +F SKNKAAQM   SR  SELSTRDLQK+VQALPQY EQ++KLS HVE+A K+N+I
Sbjct: 361 EKMTNFASKNKAAQMR--SRDGSELSTRDLQKIVQALPQYGEQVDKLSTHVELAGKINRI 420

Query: 421 IKDEGLRELGQLEQDLVFGDAGTKDVIKFLTTNEDVSRENKLRLLMILAAIYPEKFEGEK 480
           I+D GLR+LGQLEQDLVFGDAG KDVI FL TN+D + ENKLRLLMI A +YPEKFEG+K
Sbjct: 421 IRDTGLRDLGQLEQDLVFGDAGAKDVINFLRTNQDTNPENKLRLLMIYATVYPEKFEGDK 480

Query: 481 GLNLMKLAKLPPDDMNAVTNMRLLGGAAPDSKKSSLGSFSLKFDIHKKKRAVRKQLKGEE 540
           G+ LM+LA+L P DM  ++NM+L+ G +P++K  S GSFSLKFD  K K+A RK   GEE
Sbjct: 481 GVKLMQLARLSPVDMKVISNMQLIAG-SPENKAKS-GSFSLKFDAGKTKQANRKDRSGEE 540

Query: 541 ETWQLSRFYPIIEELVEKLSKGELSKEDYPCLNDPSQTYHGPSHTAAVQQ--LPSA---- 600
           ETWQL RFYP+IEEL+EKL KG+LSK DY C+N  S      + T +V++   P+A    
Sbjct: 541 ETWQLFRFYPMIEELLEKLVKGDLSKSDYLCMNQSSHKEESEARTGSVRKSSAPTAVPER 600

Query: 601 ----HSMRSRRTPTWARPRNSDDGYSSDSVLRHASSDFKKMGQRIFVFIVGGATRSELRV 660
               HSMRSRRT TWA             +L+ AS++FKK+GQRIFVFI+GGATRSELRV
Sbjct: 601 KATPHSMRSRRTATWA-------------LLKSASTEFKKLGQRIFVFIIGGATRSELRV 647

Query: 661 CHKLTSKLKREVVLGSTSIDDPPQFITKLKMLTAQEL 684
           CHKLTS L+REVVLGSTS DDPPQ+ITKLK+L+ +++
Sbjct: 661 CHKLTSSLRREVVLGSTSFDDPPQYITKLKLLSEKDI 647

BLAST of Cp4.1LG05g06840 vs. TAIR 10
Match: AT1G77140.1 (vacuolar protein sorting 45 )

HSP 1 Score: 109.0 bits (271), Expect = 1.5e-23
Identity = 121/544 (22.24%), Postives = 232/544 (42.65%), Query Frame = 0

Query: 41  STWKVLIMDKLTVKIMSYACKMADITDEGVSLVE---DIYRRRQPLPSMDAIYFLQPSRE 100
           S  KVLI+D  TV  +S     +++  + V LVE    I   ++ +  + A+YF++P+ +
Sbjct: 19  SGMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDSISVSKESMSHLKAVYFIRPTSD 78

Query: 101 NVVMFLADMSGRSPLYRKAFVFFSSPISRELVTQIKRDSTVLPRIAALKEMNLEYFAIDS 160
           N+      ++  +P + +  +FFS+ + ++    I  DS     +  ++E   ++ + D 
Sbjct: 79  NIQKLRYQLA--NPRFGEYHLFFSN-LLKDTQIHILADSDEQEVVQQVQEYYADFVSGDP 138

Query: 161 QGFITNSEKALEELFCDDESSQKGAACLNETAIRIGTVFASLRVLFVLDYVMFGLVGVSD 220
             F  N   A   L+                   I  V     +    D V+ G+  V  
Sbjct: 139 YHFTLN--MASNHLY------------------MIPAVVDPSGLQRFSDRVVDGIAAV-- 198

Query: 221 LPNLFLLQEFPSVRYQAAKSLDATTMTTFRDLIPTKVAAGVYDYITNYKKTIPNFPQSET 280
                 L+  P +RYQ                   ++A      +  ++  + +F ++E+
Sbjct: 199 ---FLALKRRPVIRYQRTSD------------TAKRIAHETAKLMYQHESALFDFRRTES 258

Query: 281 CE-LLILDRSVDQIAPVMHEWTYDAMCRDLLSMEGNKYVHEIPSKVGGPPEKKEVLLEDH 340
              LL++DR  D + P++++WTY AM  +L+ ++ NK   +    +    + + VL  + 
Sbjct: 259 SPLLLVIDRRDDPVTPLLNQWTYQAMVHELIGLQDNKVDLKSIGSLPKDQQVEVVLSSEQ 318

Query: 341 DPVWLELRHAHIADASVRLHEKMAHFVSKNKAAQMHQVSRSTSELST-RDLQKMVQALPQ 400
           D  +    + +  D  + +         K       QV++S   + T  D+ + V   P+
Sbjct: 319 DAFFKSNMYENFGDIGMNI---------KRMVDDFQQVAKSNQNIQTVEDMARFVDNYPE 378

Query: 401 YSEQIEKLSLHVEIAVKLNKIIKDEGLRELGQLEQDLVF--GDAGTKDVIKFLTTNEDVS 460
           Y +    +S HV +  +++K+++   L  + Q EQDL    G     + +  L  NE VS
Sbjct: 379 YKKMQGNVSKHVTLVTEMSKLVEARKLMTVSQTEQDLACNGGQGAAYEAVTDLLNNESVS 438

Query: 461 RENKLRLLMILAAIYPEKFEGEKGLNLM----KLAKLPPDDMNAVTNMRLLGGAAPDSKK 520
             ++LRL+M    +Y  ++E E  + LM    KLA   P     +    LL  A  + + 
Sbjct: 439 DIDRLRLVM----LYALRYEKENPVQLMQLFNKLASRSPKYKPGLVQF-LLKQAGVEKRT 498

Query: 521 SSLGSFSLKFDIHKKKRAVRKQLKGEEETWQLSRFYPIIEELVEKLSKGELSKEDYPCLN 574
             L       D+    R + + LKG E  +  ++  P++ + +E +++G L   DYP + 
Sbjct: 499 GDLFG---NRDLLNIARNMARGLKGVENVY--TQHQPLLFQTMESITRGRLRDVDYPFVG 503