Cp4.1LG05g06550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g06550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG05: 3960340 .. 3964555 (-)
RNA-Seq ExpressionCp4.1LG05g06550
SyntenyCp4.1LG05g06550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGCAACACGTGGTTGTGTGGATAGGTCACGTGAAAAAACTGGGAATCGCAAAATTTAGTCTCCGAGTTCAAATTTTCGCAGTAATTAACAGTTTTCTGGCTGAACATTCTCTCCCTTTCAATAATCCCAGCCACCATGTTCTTCCCCAGATCAAATTTTCCCCTTCAATGCACGCAATCTTCCAAATTCATAAACCCTAATACAGGCGTTGACTTCCAGGTAGGTATAATCCTTCCTTGTCTATCTTTGGATTTATATGCATTTCTTATTTGGCTTTCTATGAATAGCTCTTAGCTTCCCAGCATTGACTCGATTTCTCTGTCTCGATAGTTAACTTCTAGAAATCCAGCGATTAAAGAACCTTTTCAATCTTTGAAATCCTGTTTTTCAGTTCTAAAATGTTCTGTGTATGATTCTGTTTCGCAAAAAATTCGAAACCCAGAACCTCGTTTGTCTTGATTTTCATTCCCGAATTAGTTTAGTTTCATGATCTTCAATGATTGGAAGCTACTGGAATGTTGATATGATGTTGGTTGCTGAATTGATCCGTTTTTATTGTAATATGTTTCTTGATATGTCTTACATTTACTAGGAACATCCGCATGATTTATCGTTCCTTTGAGGCTAGATCATAGGCATCAATGTCTATCATATGTGGCGTGCCTATCCTTGAGTGTGTATGCTGTCTGGGATGTTCTCGTTGGGTCTGGAAACGTTGTCTGCACACAGCCGGTCATGACAGTGAAAATTGGGGCTTAGCCACTTCCGATGAGTTTGAGCCTATTCCCCGGGTTTGTAGATATATCCTAGCTGTGTATGAAGATGATATTCGAAAGCCCCTTTGGGAACCGGTGGGTGGTTATGGAATCAATCCAGATTTGTTACTTGTGAAGAAGACATACAAAGATACACGAGGGCGGGCTCCTCCATATATTTTATATCTTGATCATGATCATGGCGATATTGTTCTTGCTATCAGGGGACTTAATATGGCAAAGGAGAGTGATTATGCTGTTTTACTAGACAATAAGCTGGGAAAGAAGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGGTGGGTTTTGGACACTGAGAATGAAACTTTAAAGGATTTGGTGAAGAAGTATCCAGACTATACTTTGACGTTTGCTGGGCATTCTCTTGGCTCCGGAGTAGCAGCCATGTTAACTTTGGTAGTTGCACAGAATCTCGAAAAATTGGAAAATATTGATCGGAAGCGGATAAGGTGCTATGCTATTGCTCCTGCTAGATGCATGTCCCTGAATCTGGCTGTTAGATATGCAGATATCATCCACTCTGTTGTTCTTCAGGTAAACTGTGTATGTTCTTAATTTTTTTTATTTTACAAAGCATTCTGATAAGTCCTAGGATTTGTCTGTGAATCTCTTCTATAACTACAAGGCTTCTTTGTTATTTCTGAAATCACACGTTGGTTGGAGAGGGAAACGAAACATTCCTTATAAGGGTGTGAAAACCTCTCCCTAGTTGACACACTTTAAAATCTTGAAAGGAAGCTCGGAAGGGAAAGCCCAAAGAAGGCAATATCTGCTAGTGGTGAGCTTGGACTATTAGAAATGGTATCAGAGTCAGACACCGGACGGTGTGCCAGCGAGGAAGCTAGCCCCCAAAGGAGTTGGATTATGAGATCCCACATTAGTTGGAGAGTGAAACGAAACATTCTTTATAAAGGTGTGGAAACCTCTCTCTAGTAGACGCGTTTTAAAACCTTGAGGGGAAGCCGGGAAGGGAGAGTCCAAAGAGGACAATATCTACTAGTGGTGGGCTTGAACAGTTAGTAATGGTATCAGAGCCCAAACACTGGGCAGTGTCTCAGCGAGGACGTTGGACTCTCAAAGGTGGTGGATTGTAAGATCTCACATCGGTTGGAGAGGGGAATGAAACATTTCTTATAAGGGTGTGGAAACCTCTCTCTAGTAGATGCTTTTAAAATCTTGAGGGGAAACCAAAAGGAAAAATCCAAAGGGACAACATCCGCTAGCAATGGATTTGGATTTGGATTTGGGCTTGGGCTGTTACATTATCATGTCATGGAAGTGTGCTCAATATGTGCAGAATTTATGCTTGCTAATTGAAATGTAACGGTGATTATTATTCTGATCCAGGATGACTTCTTACCCAGGACAGCCACCCCCTTGGAAGACATTTTTAAGTCACTACTCTGGTATGGGTTATGATTCATGTCTCTCTATGGTGTTAATACTTTTTAGGTTGTTGGTAAAGTAAATATTGCTTGCAAACATAGAGAGCTCTATTCTTGCAGTGTGAACTGATTCTTATGTTAAAGATCATTGCATAGAAGTAACAACAGAAACTTTGCATTTTTGTGAAAGTAAAAGATTGGAAATGGAAGCTTGGATGTATTCAGTATGATTATAAGTTTCTCAATAATCATTTGAAATTATCCCTTCCCCAACAATGAATATTACATAAATTGGTTCGTTTTCCTTGTAGTTTGCCATGCCTTCTATGCCTGAGGTGCCTGCGGGATACGTGTGTATCAGAGGACCGGATGCTTAAAGATCCGAGGAGGCTTTATGCACCTGGTCGACTCTATCACATCGTCGAGCGAAAGCCCTTCAGGTAAAAACGACATGTATTTTCCGTATTAAGTTGGTTAATGTTGTGGAGAATTCTTGCGTCCCTTCAAGTTGGTGAAATTTTGTTTCATGTTCCGTTCAAACAAGAAAACATACCAGTTAAGACTGTGCTTGTTATGTGGATAGAATTTATTGTTTTATTACAAGCATGTGTGTTCATAAAGTTGAGACACTGAGTTTATGACCTTCCTATACTTAATGTGAGGAAAGGAAGCCTCTTTTAACTTATAATATTTTCCTTTTGGACTTTCTCTTCTGGGATTCCCTTCAAGATTTTTAAAACGCGCCTACTAGGGAGAGGTTTCCACACCCTTATTAAGAATGTTTTGTTCCCCTCTCCAACCGATGTGGGATCTCACCATCCACCTCCCTTCAAGGTCCAACGTCCTCGCTGGCACACCCGCCTATTATCTACCCCACTTCAGGGTTCAGCCTCCTCGTTGGCATACTGCCCGGTATCCACCCATTCAGGGTCTGGCCTCCTTGCTGACACATCGCCCGGTGTCCACCCCCTTCAGAGCTTAGTGTCTCGCTAGCACACCGCTTGGTGTCCACCTCCCTTCAAGGCTCAGCGTCCTTGCTGGCACACTGCCCGGTGTTAATCCCGATCCCACATCGGTTGGAGAGAGAAATGAAACATTTTTTATAAGTGTGTGGAAACCTCTTCCTAGCATATTCGTTTTAAAATCTTGAGGGGAATTCTGAAAGAAAAAACCCAAAAAAGACAATATCTGCTAGCGACGGGCTTAGGATGTTACGTTAATGTGGCTTTCTCAACCATTGAAACATGTCCTGCTCTAACATTGAATGGATTCACCTGGTGTTTCTTGTTGAAAGATGCGGAAGGTTTCCACCTGTGGTGAAGACGGCTGTTCCAGTAGATGGGCGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCTGACCATGCCATCATTTGGATAGAGAAAGAAGCCAAACAAGCCCTGGAAGTAAGTTCCATCATTCTACCAAGCCATTTATGCCGACTGCTTCAAGTTTAATGGAAATTTGAAATACAACACAACCATTTGCTTCTGTCAACTACAGTTAATGCACAAGGATGATAAAGCCATGCAGATACCACCCCAACAAAAGATGGAGAGGCAAGAGACTTTAGCTAGAGAGCATAGCGAAGAGTATAAGGCTGCATTGCAACGTGCCGTGACGTTAGCAGTGCCACATGCCTACGCGCTTGCCCCATACGGGACCTTCGACCAGACAGATGATGGGGAAGAAGAAGAAGAGTCACCAGGATCAAGTGGAGGGTCGTCGAGGAGGAAGAAAGAAACTTGGAATGAACTGATAGAACGTCTTTACGACAAGGATGATTCAAGAAGCGCAGTGCTGAAGAAATCAGTGAGTAGTATTTGATGTTTCATTGTGGCTATTCATATGAATATTTGTTGCAATACTTTTGTATAATAACGTATACTTGCACTTTGTTCGTACTTATTATTTGCTACTTGTGATACTATATACTTGCTCAATTCGAGTAAATATTGTCTACTTTGCTTTGGATTGTTAGGGGAGGTTTTCAC

mRNA sequence

TAGCAACACGTGGTTGTGTGGATAGGTCACGTGAAAAAACTGGGAATCGCAAAATTTAGTCTCCGAGTTCAAATTTTCGCAGTAATTAACAGTTTTCTGGCTGAACATTCTCTCCCTTTCAATAATCCCAGCCACCATGTTCTTCCCCAGATCAAATTTTCCCCTTCAATGCACGCAATCTTCCAAATTCATAAACCCTAATACAGGCGTTGACTTCCAGGAACATCCGCATGATTTATCGTTCCTTTGAGGCTAGATCATAGGCATCAATGTCTATCATATGTGGCGTGCCTATCCTTGAGTGTGTATGCTGTCTGGGATGTTCTCGTTGGGTCTGGAAACGTTGTCTGCACACAGCCGGTCATGACAGTGAAAATTGGGGCTTAGCCACTTCCGATGAGTTTGAGCCTATTCCCCGGGTTTGTAGATATATCCTAGCTGTGTATGAAGATGATATTCGAAAGCCCCTTTGGGAACCGGTGGGTGGTTATGGAATCAATCCAGATTTGTTACTTGTGAAGAAGACATACAAAGATACACGAGGGCGGGCTCCTCCATATATTTTATATCTTGATCATGATCATGGCGATATTGTTCTTGCTATCAGGGGACTTAATATGGCAAAGGAGAGTGATTATGCTGTTTTACTAGACAATAAGCTGGGAAAGAAGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGGTGGGTTTTGGACACTGAGAATGAAACTTTAAAGGATTTGGTGAAGAAGTATCCAGACTATACTTTGACGTTTGCTGGGCATTCTCTTGGCTCCGGAGTAGCAGCCATGTTAACTTTGGTAGTTGCACAGAATCTCGAAAAATTGGAAAATATTGATCGGAAGCGGATAAGGTGCTATGCTATTGCTCCTGCTAGATGCATGTCCCTGAATCTGGCTGTTAGATATGCAGATATCATCCACTCTGTTGTTCTTCAGGATGACTTCTTACCCAGGACAGCCACCCCCTTGGAAGACATTTTTAAGTCACTACTCTGTTTGCCATGCCTTCTATGCCTGAGGTGCCTGCGGGATACGTGTGTATCAGAGGACCGGATGCTTAAAGATCCGAGGAGGCTTTATGCACCTGGTCGACTCTATCACATCGTCGAGCGAAAGCCCTTCAGATGCGGAAGGTTTCCACCTGTGGTGAAGACGGCTGTTCCAGTAGATGGGCGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCTGACCATGCCATCATTTGGATAGAGAAAGAAGCCAAACAAGCCCTGGAATTAATGCACAAGGATGATAAAGCCATGCAGATACCACCCCAACAAAAGATGGAGAGGCAAGAGACTTTAGCTAGAGAGCATAGCGAAGAGTATAAGGCTGCATTGCAACGTGCCGTGACGTTAGCAGTGCCACATGCCTACGCGCTTGCCCCATACGGGACCTTCGACCAGACAGATGATGGGGAAGAAGAAGAAGAGTCACCAGGATCAAGTGGAGGGTCGTCGAGGAGGAAGAAAGAAACTTGGAATGAACTGATAGAACGTCTTTACGACAAGGATGATTCAAGAAGCGCAGTGCTGAAGAAATCAGTGAGTAGTATTTGATGTTTCATTGTGGCTATTCATATGAATATTTGTTGCAATACTTTTGTATAATAACGTATACTTGCACTTTGTTCGTACTTATTATTTGCTACTTGTGATACTATATACTTGCTCAATTCGAGTAAATATTGTCTACTTTGCTTTGGATTGTTAGGGGAGGTTTTCAC

Coding sequence (CDS)

ATGTCTATCATATGTGGCGTGCCTATCCTTGAGTGTGTATGCTGTCTGGGATGTTCTCGTTGGGTCTGGAAACGTTGTCTGCACACAGCCGGTCATGACAGTGAAAATTGGGGCTTAGCCACTTCCGATGAGTTTGAGCCTATTCCCCGGGTTTGTAGATATATCCTAGCTGTGTATGAAGATGATATTCGAAAGCCCCTTTGGGAACCGGTGGGTGGTTATGGAATCAATCCAGATTTGTTACTTGTGAAGAAGACATACAAAGATACACGAGGGCGGGCTCCTCCATATATTTTATATCTTGATCATGATCATGGCGATATTGTTCTTGCTATCAGGGGACTTAATATGGCAAAGGAGAGTGATTATGCTGTTTTACTAGACAATAAGCTGGGAAAGAAGAAATTTGATGGTGGATATGTTCACAATGGGCTTCTGAAGGCAGCTGGGTGGGTTTTGGACACTGAGAATGAAACTTTAAAGGATTTGGTGAAGAAGTATCCAGACTATACTTTGACGTTTGCTGGGCATTCTCTTGGCTCCGGAGTAGCAGCCATGTTAACTTTGGTAGTTGCACAGAATCTCGAAAAATTGGAAAATATTGATCGGAAGCGGATAAGGTGCTATGCTATTGCTCCTGCTAGATGCATGTCCCTGAATCTGGCTGTTAGATATGCAGATATCATCCACTCTGTTGTTCTTCAGGATGACTTCTTACCCAGGACAGCCACCCCCTTGGAAGACATTTTTAAGTCACTACTCTGTTTGCCATGCCTTCTATGCCTGAGGTGCCTGCGGGATACGTGTGTATCAGAGGACCGGATGCTTAAAGATCCGAGGAGGCTTTATGCACCTGGTCGACTCTATCACATCGTCGAGCGAAAGCCCTTCAGATGCGGAAGGTTTCCACCTGTGGTGAAGACGGCTGTTCCAGTAGATGGGCGGTTTGAACACATAGTTCTTTCTTGTAATGCAACTTCTGACCATGCCATCATTTGGATAGAGAAAGAAGCCAAACAAGCCCTGGAATTAATGCACAAGGATGATAAAGCCATGCAGATACCACCCCAACAAAAGATGGAGAGGCAAGAGACTTTAGCTAGAGAGCATAGCGAAGAGTATAAGGCTGCATTGCAACGTGCCGTGACGTTAGCAGTGCCACATGCCTACGCGCTTGCCCCATACGGGACCTTCGACCAGACAGATGATGGGGAAGAAGAAGAAGAGTCACCAGGATCAAGTGGAGGGTCGTCGAGGAGGAAGAAAGAAACTTGGAATGAACTGATAGAACGTCTTTACGACAAGGATGATTCAAGAAGCGCAGTGCTGAAGAAATCAGTGAGTAGTATTTGA

Protein sequence

MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYEDDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKESDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLPRTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCGRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKMERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRRKKETWNELIERLYDKDDSRSAVLKKSVSSI
Homology
BLAST of Cp4.1LG05g06550 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-08
Identity = 49/169 (28.99%), Postives = 77/169 (45.56%), Query Frame = 0

Query: 96  PYILYLDHDHGDIVLAIRGLNMAKESDYAVLLDNKLGKKKFD------GGYVHNGLLKAA 155
           P+++ LDH    +V+A+RG    ++    VL D     +  D          H G+ +AA
Sbjct: 357 PFLVALDHRKESVVVAVRGTMSLQD----VLTDLSAESEVLDVECEVQDRLAHKGISQAA 416

Query: 156 GWVLD--TENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVAQNLEKLENIDRKRIR 215
            +V      +  L       P+Y L   GHSLG G AA+L  ++             ++R
Sbjct: 417 RYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATML--------RAAYPQVR 476

Query: 216 CYAIAPARCMSLNLAVRYA-DIIHSVVLQDDFLPR-TATPLEDIFKSLL 255
           CYA +P R +       Y+   I S+VL  D +PR + T LED+ + +L
Sbjct: 477 CYAFSPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRIL 513

BLAST of Cp4.1LG05g06550 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 3.4e-08
Identity = 47/169 (27.81%), Postives = 81/169 (47.93%), Query Frame = 0

Query: 96  PYILYLDHDHGDIVLAIRGLNMAKESDYAVLLDNKLGKKKFDGG------YVHNGLLKAA 155
           P+I+ LDH    +V+A+RG    ++    VL D     +  + G        H G+ +AA
Sbjct: 357 PFIVVLDHRKESVVVAVRGTMSLQD----VLTDLSAESETLELGIELQDCVAHKGIAQAA 416

Query: 156 GWVLD--TENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVAQNLEKLENIDRKRIR 215
            ++      +  L       P+Y L   GHSLG+G AA+L +++             ++R
Sbjct: 417 RYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAY--------PQVR 476

Query: 216 CYAIAPAR-CMSLNLAVRYADIIHSVVLQDDFLPR-TATPLEDIFKSLL 255
            YA +P R  +S +L     D + S++L  D +PR + T +ED+ + +L
Sbjct: 477 AYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLSVTNMEDLKRRIL 513

BLAST of Cp4.1LG05g06550 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.4e-08
Identity = 44/165 (26.67%), Postives = 80/165 (48.48%), Query Frame = 0

Query: 96  PYILYLDHDHGDIVLAIRGLNMAKE--SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVL 155
           P+I+ LDH    +V+A+RG    ++  +D +   +N     +      H G+ +AA ++ 
Sbjct: 357 PFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIY 416

Query: 156 D--TENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVAQNLEKLENIDRKRIRCYAI 215
                +  L       P+Y L   GHSLG+G AA+L +++             ++R YA 
Sbjct: 417 RRLVNDGILSQAFSVAPEYRLVVVGHSLGAGAAALLAIMLRGAY--------PQVRAYAF 476

Query: 216 APAR-CMSLNLAVRYADIIHSVVLQDDFLPR-TATPLEDIFKSLL 255
           +P R  +S +L     D + S++L  D +PR +   +ED+ + +L
Sbjct: 477 SPPRGLLSKSLFEYSKDFVVSLILGMDVIPRLSVANMEDLKRRIL 513

BLAST of Cp4.1LG05g06550 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 6.6e-04
Identity = 30/97 (30.93%), Postives = 47/97 (48.45%), Query Frame = 0

Query: 99  LYLDHDHGDIVLAIRGLNMAKESDYAVLLDNKLGKKKFD---------GGYVHNGLLKAA 158
           L LD+ +  IVL+ RG            ++N +G   FD         G   H+G   + 
Sbjct: 89  LALDNTNKLIVLSFRGSRS---------IENWIGNLNFDLKEINDICSGCRGHDGFTSSW 148

Query: 159 GWVLDTENETLKDLVKKYPDYTLTFAGHSLGSGVAAM 187
             V DT  + ++D V+++PDY + F GHSLG  +A +
Sbjct: 149 RSVADTLRQKVEDAVREHPDYRVVFTGHSLGGALATV 176

BLAST of Cp4.1LG05g06550 vs. ExPASy Swiss-Prot
Match: Q9P979 (Feruloyl esterase A OS=Aspergillus awamori OX=105351 GN=faeA PE=1 SV=3)

HSP 1 Score: 46.6 bits (109), Expect = 8.6e-04
Identity = 38/108 (35.19%), Postives = 46/108 (42.59%), Query Frame = 0

Query: 102 DHDHGDIVLAIRGLNMAKESDYAVLLDNKLGKKKFD------GGYVHNGLLKAAGW--VL 161
           D    +I+   RG      SD  + LD       FD      G  VH G     GW  V 
Sbjct: 76  DDSSKEIITVFRGTG----SDTNLQLDTNYTLTPFDTLPQCNGCEVHGGYY--IGWVSVQ 135

Query: 162 DTENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVAQNLEKLENI 202
           D     +K  V +YPDY LT  GHSLG+ +AA   L  AQ     +NI
Sbjct: 136 DQVESLVKQQVSQYPDYALTVTGHSLGASLAA---LTAAQLSATYDNI 174

BLAST of Cp4.1LG05g06550 vs. NCBI nr
Match: XP_023532070.1 (uncharacterized protein LOC111794345 [Cucurbita pepo subsp. pepo] >XP_023532071.1 uncharacterized protein LOC111794345 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR 420

Query: 421 KKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           KKETWNELIERLYDKDDSRSAVLKKSVSSI
Sbjct: 421 KKETWNELIERLYDKDDSRSAVLKKSVSSI 450

BLAST of Cp4.1LG05g06550 vs. NCBI nr
Match: KAG7035639.1 (Sn1-specific diacylglycerol lipase alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 910 bits (2352), Expect = 0.0
Identity = 445/450 (98.89%), Postives = 448/450 (99.56%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTF +TDDGEEEEESPGSSGGSSRR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFGKTDDGEEEEESPGSSGGSSRR 420

Query: 421 KKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           KKETWNELIERLYDK+DS+ AVLKKSVSSI
Sbjct: 421 KKETWNELIERLYDKEDSKHAVLKKSVSSI 450

BLAST of Cp4.1LG05g06550 vs. NCBI nr
Match: XP_022995690.1 (uncharacterized protein LOC111491150 [Cucurbita maxima] >XP_022995691.1 uncharacterized protein LOC111491150 [Cucurbita maxima])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 446/451 (98.89%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLA RGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAFRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKP RCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPLRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKA+QIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKALQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGG-SSR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTF QTDDGEEEEESPGSSGG SSR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFGQTDDGEEEEESPGSSGGGSSR 420

Query: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           RKKETWNELIERLYDKDDSRSAVLKKSVSSI
Sbjct: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 451

BLAST of Cp4.1LG05g06550 vs. NCBI nr
Match: KAG6605732.1 (Sn1-specific diacylglycerol lipase alpha, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 445/451 (98.67%), Postives = 448/451 (99.33%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEE-SPGSSGGSSR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTF +TDDGEEEEE SPGSSGGSSR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFGKTDDGEEEEEESPGSSGGSSR 420

Query: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           RKKETWNELIERLYDK+DS+ AVLKKSVSSI
Sbjct: 421 RKKETWNELIERLYDKEDSKHAVLKKSVSSI 451

BLAST of Cp4.1LG05g06550 vs. NCBI nr
Match: XP_022958038.1 (uncharacterized protein LOC111459390 [Cucurbita moschata] >XP_022958039.1 uncharacterized protein LOC111459390 [Cucurbita moschata])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 444/451 (98.45%), Postives = 448/451 (99.33%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKD+KAMQIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDNKAMQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEE-SPGSSGGSSR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTF +TDDGEEEEE SPGSSGGSSR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFGKTDDGEEEEEESPGSSGGSSR 420

Query: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           RKKETWNELIERLYDK+DS+ AVLKKSVSSI
Sbjct: 421 RKKETWNELIERLYDKEDSKHAVLKKSVSSI 451

BLAST of Cp4.1LG05g06550 vs. ExPASy TrEMBL
Match: A0A6J1K4N8 (uncharacterized protein LOC111491150 OS=Cucurbita maxima OX=3661 GN=LOC111491150 PE=4 SV=1)

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 446/451 (98.89%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLA RGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAFRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKP RCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPLRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKA+QIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKALQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGG-SSR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTF QTDDGEEEEESPGSSGG SSR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFGQTDDGEEEEESPGSSGGGSSR 420

Query: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           RKKETWNELIERLYDKDDSRSAVLKKSVSSI
Sbjct: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 451

BLAST of Cp4.1LG05g06550 vs. ExPASy TrEMBL
Match: A0A6J1H3W0 (uncharacterized protein LOC111459390 OS=Cucurbita moschata OX=3662 GN=LOC111459390 PE=4 SV=1)

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 444/451 (98.45%), Postives = 448/451 (99.33%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE
Sbjct: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKD+KAMQIPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDNKAMQIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEE-SPGSSGGSSR 420
           ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTF +TDDGEEEEE SPGSSGGSSR
Sbjct: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFGKTDDGEEEEEESPGSSGGSSR 420

Query: 421 RKKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           RKKETWNELIERLYDK+DS+ AVLKKSVSSI
Sbjct: 421 RKKETWNELIERLYDKEDSKHAVLKKSVSSI 451

BLAST of Cp4.1LG05g06550 vs. ExPASy TrEMBL
Match: A0A1S3ASL8 (uncharacterized protein LOC103482554 OS=Cucumis melo OX=3656 GN=LOC103482554 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 2.16e-312
Identity = 411/450 (91.33%), Postives = 433/450 (96.22%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSI+CGVPILECVCCLGC+RWVWKRCLHTAGHDSENWG AT DEFEPIPR+CRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPD L++KKTYKDTRGRAPPYILYLDHDH DIVLAIRGLNM KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVV QN EKLENIDRKRIRCYAIAPARCMSLNLAVRYAD+I+SVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSL CLPCLLCLRC+RDTCVSE++MLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK+ALELM KDDK M+IPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR 420
           ERQ+TLAREHSEEYKAALQRAVTLAVPHAYA++PYGTF QTD+GEEE+ SP SSGGSSRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK-SPASSGGSSRR 420

Query: 421 KKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           +KETW+ELIERLYDKDDSR  VLKKS+S I
Sbjct: 421 RKETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cp4.1LG05g06550 vs. ExPASy TrEMBL
Match: A0A5A7TNW3 (Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00050 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 2.16e-312
Identity = 411/450 (91.33%), Postives = 433/450 (96.22%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSI+CGVPILECVCCLGC+RWVWKRCLHTAGHDSENWG AT DEFEPIPR+CRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPD L++KKTYKDTRGRAPPYILYLDHDH DIVLAIRGLNM KE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLIMKKTYKDTRGRAPPYILYLDHDHADIVLAIRGLNMVKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTF GHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFTGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTLVV QN EKLENIDRKRIRCYAIAPARCMSLNLAVRYAD+I+SVVLQDDFLP
Sbjct: 181 SGVAAMLTLVVVQNREKLENIDRKRIRCYAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSL CLPCLLCLRC+RDTCVSE++MLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK+ALELM KDDK M+IPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKRALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR 420
           ERQ+TLAREHSEEYKAALQRAVTLAVPHAYA++PYGTF QTD+GEEE+ SP SSGGSSRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK-SPASSGGSSRR 420

Query: 421 KKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           +KETW+ELIERLYDKDDSR  VLKKS+S I
Sbjct: 421 RKETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cp4.1LG05g06550 vs. ExPASy TrEMBL
Match: A0A0A0KRF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166450 PE=4 SV=1)

HSP 1 Score: 851 bits (2199), Expect = 5.07e-311
Identity = 411/450 (91.33%), Postives = 433/450 (96.22%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSI+CGVPILECVCCLGC+RWVWKRCLHTAGHDSENWG AT DEFEPIPR+CRYIL+VYE
Sbjct: 1   MSILCGVPILECVCCLGCARWVWKRCLHTAGHDSENWGFATPDEFEPIPRICRYILSVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           DDIRKPLWEPVGGYGINPD LL+KKTYKDTRGRAPPYILYLDH H DIVLAIRGLNMAKE
Sbjct: 61  DDIRKPLWEPVGGYGINPDWLLMKKTYKDTRGRAPPYILYLDHVHADIVLAIRGLNMAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
           SDYAVLLDN+LGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG
Sbjct: 121 SDYAVLLDNRLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           SGVAAMLTL+V QN EKLENIDRKRIRC+AIAPARCMSLNLAVRYAD+I+SVVLQDDFLP
Sbjct: 181 SGVAAMLTLLVVQNREKLENIDRKRIRCFAIAPARCMSLNLAVRYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RTATPLEDIFKSL CLPCLLCLRC+RDTCVSE++MLKDPRRLYAPGRLYHIVERKPFRCG
Sbjct: 241 RTATPLEDIFKSLFCLPCLLCLRCVRDTCVSEEKMLKDPRRLYAPGRLYHIVERKPFRCG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAK ALELM KDDK M+IPPQQKM
Sbjct: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKWALELMRKDDKIMEIPPQQKM 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSGGSSRR 420
           ERQ+TLAREHSEEYKAALQRAVTLAVPHAYA++PYGTF QTD+GEEE+ SP SSGGSSRR
Sbjct: 361 ERQQTLAREHSEEYKAALQRAVTLAVPHAYAISPYGTFSQTDEGEEEK-SPASSGGSSRR 420

Query: 421 KKETWNELIERLYDKDDSRSAVLKKSVSSI 450
           +KETW+ELIERLYDKDDSR  VLKKS+S I
Sbjct: 421 RKETWDELIERLYDKDDSRHTVLKKSLSCI 449

BLAST of Cp4.1LG05g06550 vs. TAIR 10
Match: AT3G49050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 684.5 bits (1765), Expect = 5.8e-197
Identity = 334/473 (70.61%), Postives = 398/473 (84.14%), Query Frame = 0

Query: 1   MSIICG-VPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVY 60
           MSI+CG  P+LECV CLGC+RW +KRCL+TAGHDSE+WGLAT+DEFEP+PR CRYILAVY
Sbjct: 1   MSILCGCCPLLECVYCLGCARWGYKRCLYTAGHDSEDWGLATTDEFEPVPRFCRYILAVY 60

Query: 61  EDDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAK 120
           EDDIR PLWEP  GYGINPD LL+KKTY+DT+GRAP YILYLDH H DIV+AIRGLN+AK
Sbjct: 61  EDDIRNPLWEPPEGYGINPDWLLLKKTYEDTQGRAPAYILYLDHVHQDIVVAIRGLNLAK 120

Query: 121 ESDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSL 180
           ESDYA+LLDNKLG++KFDGGYVHNGL+K+AG+VLD E + LK+LVKKYP YTLTFAGHSL
Sbjct: 121 ESDYAMLLDNKLGERKFDGGYVHNGLVKSAGYVLDEECKVLKELVKKYPSYTLTFAGHSL 180

Query: 181 GSGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFL 240
           GSGVA ML L+V ++ E+L NIDRKR+RC+AIAPARCMSLNLAVRYAD+I+SV+LQDDFL
Sbjct: 181 GSGVATMLALLVVRHPERLGNIDRKRVRCFAIAPARCMSLNLAVRYADVINSVILQDDFL 240

Query: 241 PRTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRC 300
           PRTATPLEDIFKS+ CLPCLLC+RC++DTCV E +MLKDPRRLYAPGR+YHIVERKP R 
Sbjct: 241 PRTATPLEDIFKSVFCLPCLLCIRCMKDTCVPEQKMLKDPRRLYAPGRMYHIVERKPCRL 300

Query: 301 GRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQK 360
           GR+PPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIE+EA++AL LM +++K M+IP +Q+
Sbjct: 301 GRYPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEREAQRALNLMMENEKKMEIPEKQR 360

Query: 361 MERQETLAREHSEEYKAALQRAVTLAVPHAYALA-PYGTFDQTDDGEEEEES-------- 420
           MERQE+LAREH+ EY+AAL+RAVTL VPHA ++A  YGTFD+T + E EEE         
Sbjct: 361 MERQESLAREHNLEYRAALRRAVTLDVPHAESMAYEYGTFDKTQEDETEEEEVETEEEEE 420

Query: 421 --------PGSSGGSS---------RRKKETWNELIERLYDKDDSRSAVLKKS 447
                    G S  SS         R ++ +W+ELIE L+++D+S +   +KS
Sbjct: 421 DTDSIAPMVGESSSSSSVKPTYRIRRNRRVSWDELIEHLFERDESGNLTFEKS 473

BLAST of Cp4.1LG05g06550 vs. TAIR 10
Match: AT4G00500.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 587.4 bits (1513), Expect = 9.6e-168
Identity = 278/452 (61.50%), Postives = 362/452 (80.09%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSI+C VP+LECV CLGC+ W+WK+CL++AGH+SENWGLATSDEFEPIPR+CR ILAVYE
Sbjct: 1   MSILCCVPVLECVYCLGCTHWLWKKCLYSAGHESENWGLATSDEFEPIPRICRLILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           +++  P+W P  GYGI+P+ +++KK Y  T GR  PY++YLDH++GD+VLAIRGLN+AKE
Sbjct: 61  ENLHDPMWAPPDGYGIDPNHVILKKDYDQTEGRVTPYMIYLDHENGDVVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
            DYAVLLDNKLG+ KFDGGYVHNGLLKAA WV + E+  L++L++  P Y+LTF GHSLG
Sbjct: 121 CDYAVLLDNKLGQTKFDGGYVHNGLLKAAMWVFEEEHVVLRELLEANPSYSLTFVGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           +GV ++L L V QN  +L NI+RKRIRC+AIAP RCMSL+LAV YAD+I+SVVLQDDFLP
Sbjct: 181 AGVVSLLVLFVIQNRVRLGNIERKRIRCFAIAPPRCMSLHLAVTYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RT T LE++FKS++CLPCLLCL CL+DT   E+R LKD RRLYAPGRLYHIV RKP R G
Sbjct: 241 RTTTALENVFKSIICLPCLLCLTCLKDTFTFEERKLKDARRLYAPGRLYHIVVRKPLRLG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           R+PPVV+TAVPVDGRFE IVLSCNAT+DHAIIWIE+E+++AL+LM ++D+ MQIP +QK+
Sbjct: 301 RYPPVVRTAVPVDGRFEQIVLSCNATADHAIIWIERESQRALDLMVEEDQVMQIPVEQKI 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEES--PGS-SGGS 420
            RQ+++  +H EEY+AA+ +A +L +P + +   YGTF  T++GE    S   GS SG S
Sbjct: 361 VRQKSIVEDHDEEYRAAIMKAASLNIPMSPS-PSYGTFHDTEEGESSAGSGMEGSPSGWS 420

Query: 421 SRRKKETWNELIERLYDKDDSRSAVLKKSVSS 450
            +  +  W++ I+  +  +D+   ++ K+  S
Sbjct: 421 FKGMRRKWDQFIDCHFPVNDNSEHMIFKNQES 451

BLAST of Cp4.1LG05g06550 vs. TAIR 10
Match: AT4G00500.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 587.4 bits (1513), Expect = 9.6e-168
Identity = 278/452 (61.50%), Postives = 362/452 (80.09%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MSI+C VP+LECV CLGC+ W+WK+CL++AGH+SENWGLATSDEFEPIPR+CR ILAVYE
Sbjct: 1   MSILCCVPVLECVYCLGCTHWLWKKCLYSAGHESENWGLATSDEFEPIPRICRLILAVYE 60

Query: 61  DDIRKPLWEPVGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE 120
           +++  P+W P  GYGI+P+ +++KK Y  T GR  PY++YLDH++GD+VLAIRGLN+AKE
Sbjct: 61  ENLHDPMWAPPDGYGIDPNHVILKKDYDQTEGRVTPYMIYLDHENGDVVLAIRGLNLAKE 120

Query: 121 SDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLG 180
            DYAVLLDNKLG+ KFDGGYVHNGLLKAA WV + E+  L++L++  P Y+LTF GHSLG
Sbjct: 121 CDYAVLLDNKLGQTKFDGGYVHNGLLKAAMWVFEEEHVVLRELLEANPSYSLTFVGHSLG 180

Query: 181 SGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLP 240
           +GV ++L L V QN  +L NI+RKRIRC+AIAP RCMSL+LAV YAD+I+SVVLQDDFLP
Sbjct: 181 AGVVSLLVLFVIQNRVRLGNIERKRIRCFAIAPPRCMSLHLAVTYADVINSVVLQDDFLP 240

Query: 241 RTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFRCG 300
           RT T LE++FKS++CLPCLLCL CL+DT   E+R LKD RRLYAPGRLYHIV RKP R G
Sbjct: 241 RTTTALENVFKSIICLPCLLCLTCLKDTFTFEERKLKDARRLYAPGRLYHIVVRKPLRLG 300

Query: 301 RFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQIPPQQKM 360
           R+PPVV+TAVPVDGRFE IVLSCNAT+DHAIIWIE+E+++AL+LM ++D+ MQIP +QK+
Sbjct: 301 RYPPVVRTAVPVDGRFEQIVLSCNATADHAIIWIERESQRALDLMVEEDQVMQIPVEQKI 360

Query: 361 ERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEES--PGS-SGGS 420
            RQ+++  +H EEY+AA+ +A +L +P + +   YGTF  T++GE    S   GS SG S
Sbjct: 361 VRQKSIVEDHDEEYRAAIMKAASLNIPMSPS-PSYGTFHDTEEGESSAGSGMEGSPSGWS 420

Query: 421 SRRKKETWNELIERLYDKDDSRSAVLKKSVSS 450
            +  +  W++ I+  +  +D+   ++ K+  S
Sbjct: 421 FKGMRRKWDQFIDCHFPVNDNSEHMIFKNQES 451

BLAST of Cp4.1LG05g06550 vs. TAIR 10
Match: AT5G37710.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 501.5 bits (1290), Expect = 7.0e-142
Identity = 253/452 (55.97%), Postives = 330/452 (73.01%), Query Frame = 0

Query: 1   MSIICGVPILECVCCLGCSRWVWKRCLHTAGHDSENWGLATSDEFEPIPRVCRYILAVYE 60
           MS+ CG   LECV C+G SRW WKRC H    DS  W  AT +EFEPIPR+ R ILAVYE
Sbjct: 1   MSVACG---LECVFCVGFSRWAWKRCTHVGSDDSATWTSATPEEFEPIPRISRVILAVYE 60

Query: 61  DDIRKPLWEP-VGGYGINPDLLLVKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAK 120
            D+R P   P +G + +NP+ ++ + T++ T+GR+PPYI+Y+DHDH +IVLAIRGLN+AK
Sbjct: 61  PDLRNPKISPSLGTFDLNPEWVIKRVTHEKTQGRSPPYIIYIDHDHREIVLAIRGLNLAK 120

Query: 121 ESDYAVLLDNKLGKKKFDGGYVHNGLLKAAGWVLDTENETL-KDLVKKYPDYTLTFAGHS 180
           ESDY +LLDNKLG+K   GGYVH GLLK+A WVL+ E+ETL +   +   +Y L FAGHS
Sbjct: 121 ESDYKILLDNKLGQKMLGGGYVHRGLLKSAAWVLNQESETLWRVWEENGREYDLVFAGHS 180

Query: 181 LGSGVAAMLTLVVAQNLEKLENIDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDF 240
           LGSGVAA++ ++V      + +I R ++RC+A+APARCMSLNLAV+YAD+I SV+LQDDF
Sbjct: 181 LGSGVAALMAVLVVNTPAMIGDIPRNKVRCFALAPARCMSLNLAVKYADVISSVILQDDF 240

Query: 241 LPRTATPLEDIFKSLLCLPCLLCLRCLRDTCVSEDRMLKDPRRLYAPGRLYHIVERKPFR 300
           LPRTATPLEDIFKS+ CLPCLL L CLRDT + E R L+DPRRLYAPGR+YHIVERK   
Sbjct: 241 LPRTATPLEDIFKSVFCLPCLLFLVCLRDTFIPEGRKLRDPRRLYAPGRIYHIVERK--- 300

Query: 301 CGRFPPVVKTAVPVDGRFEHIVLSCNATSDHAIIWIEKEAKQALELMHKDDKAMQI---P 360
             RFPP V+TA+PVDGRFEHIVLS NATSDHAI+WIE+EA++AL+++ +      +   P
Sbjct: 301 FCRFPPEVRTAIPVDGRFEHIVLSSNATSDHAILWIEREAEKALQILREKSSETVVTMAP 360

Query: 361 PQQKMERQETLAREHSEEYKAALQRAVTLAVPHAYALAPYGTFDQTDDGEEEEESPGSSG 420
            +++MER  TL +EH    K AL+RAV+L +PHA + A           EEEE + G + 
Sbjct: 361 KEKRMERLSTLEKEH----KDALERAVSLNIPHAVSTAE----------EEEECNNGEAS 420

Query: 421 GSSRRKKETWNELIERLYDKDDSRSAVLKKSV 448
              + KK+ W+E++++L+ + +S   VL  +V
Sbjct: 421 AELKTKKKNWDEVVDKLFHRSNSGEFVLNDNV 432

BLAST of Cp4.1LG05g06550 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.0e-15
Identity = 46/161 (28.57%), Postives = 81/161 (50.31%), Query Frame = 0

Query: 83  VKKTYKDTRGRAPPYILYLDHDHGDIVLAIRGLNMAKE--SDYAVLLDNKLGKKKFDGGY 142
           + K  KD+    P Y + +DH    +V  IRG +   +  +D     D ++    F+G  
Sbjct: 217 ILKFVKDSSVMRPGYYIGVDHRRKLVVFGIRGTHTIYDLITDIVSSSDEEV---TFEGYS 276

Query: 143 VHNGLLKAAGWVLDTENETLKDLVKKYPDYTLTFAGHSLGSGVAAMLTLVVAQNLEKLEN 202
            H G  +AA W L+ E +T++  + KY  Y L   GHSLG  +A+++ +++ +   +   
Sbjct: 277 THFGTAEAARWFLNHELQTIRRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELG 336

Query: 203 IDRKRIRCYAIAPARCMSLNLAVRYADIIHSVVLQDDFLPR 242
            D + I     A   C+S  LA   ++ + ++V+QDD +PR
Sbjct: 337 FDAEIISAVGYATPPCVSKELAENCSEFVTTIVMQDDIIPR 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8NCG72.0e-0828.99Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2[more]
Q91WC93.4e-0827.81Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
P0C1S94.4e-0826.67Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
O599526.6e-0430.93Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
Q9P9798.6e-0435.19Feruloyl esterase A OS=Aspergillus awamori OX=105351 GN=faeA PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_023532070.10.0100.00uncharacterized protein LOC111794345 [Cucurbita pepo subsp. pepo] >XP_023532071.... [more]
KAG7035639.10.098.89Sn1-specific diacylglycerol lipase alpha [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022995690.10.098.89uncharacterized protein LOC111491150 [Cucurbita maxima] >XP_022995691.1 uncharac... [more]
KAG6605732.10.098.67Sn1-specific diacylglycerol lipase alpha, partial [Cucurbita argyrosperma subsp.... [more]
XP_022958038.10.098.45uncharacterized protein LOC111459390 [Cucurbita moschata] >XP_022958039.1 unchar... [more]
Match NameE-valueIdentityDescription
A0A6J1K4N80.098.89uncharacterized protein LOC111491150 OS=Cucurbita maxima OX=3661 GN=LOC111491150... [more]
A0A6J1H3W00.098.45uncharacterized protein LOC111459390 OS=Cucurbita moschata OX=3662 GN=LOC1114593... [more]
A0A1S3ASL82.16e-31291.33uncharacterized protein LOC103482554 OS=Cucumis melo OX=3656 GN=LOC103482554 PE=... [more]
A0A5A7TNW32.16e-31291.33Alpha/beta-Hydrolases superfamily protein isoform 1 OS=Cucumis melo var. makuwa ... [more]
A0A0A0KRF05.07e-31191.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166450 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G49050.15.8e-19770.61alpha/beta-Hydrolases superfamily protein [more]
AT4G00500.19.6e-16861.50alpha/beta-Hydrolases superfamily protein [more]
AT4G00500.29.6e-16861.50alpha/beta-Hydrolases superfamily protein [more]
AT5G37710.17.0e-14255.97alpha/beta-Hydrolases superfamily protein [more]
AT2G42450.11.0e-1528.57alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 360..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..423
NoneNo IPR availablePANTHERPTHR46398ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..445
NoneNo IPR availablePANTHERPTHR46398:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..445
NoneNo IPR availableCDDcd00519Lipase_3coord: 44..241
e-value: 2.61493E-29
score: 112.57
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 39..251
e-value: 6.8E-28
score: 99.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 41..272
IPR005592Mono-/di-acylglycerol lipase, N-terminalPFAMPF03893Lipase3_Ncoord: 10..73
e-value: 9.3E-20
score: 70.4
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 109..243
e-value: 2.8E-23
score: 82.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g06550.1Cp4.1LG05g06550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016787 hydrolase activity