Cp4.1LG05g05060 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g05060
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein DETOXIFICATION
LocationCp4.1LG05: 2926635 .. 2931268 (-)
RNA-Seq ExpressionCp4.1LG05g05060
SyntenyCp4.1LG05g05060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAGCTTATAATTGTTAATAAATGCAAGGAAATTATAGGTAAATTACCGGCGGTGAAATTCGGTATCGAACACTGGCCGCTTTTCCTTAGCGGGAAGGACACTTGAGGCCTCTTCCCTTTGCCGCTTGCCTTTTGCCCGCCAACGCCAACGCCAACGCCAACGAATGCCTCACTTCCATTTGCTTCTCTTCGTCAACTGACCAACAAACTCGTCCTCTTCGCCTCCGACCCTCACAGGCTCCTACTAATCAAACCAATTTTTCAATACCTCAATTGGCAACTGGCTTCAGACAAAGACACCCTCTTCCCTCTTTCTTCCGTGCTTCCAATTTGAATTGGGGTTTCTTCCACGCCGTCGGAGTTGAGGTACCCAACTTGAAATGTCTTGGTGGCAATGTTTCAACCATGAGTCTCTTAGGAGGTTTATCTGTTTAGACTTTGGGTTGATTTTTTTTTTTTGTTTTCTTTTTTGGTCTAAAGGATTGTGGACCATGAGGGGCCTCCAGTTAGGAAGTTTTGCCCAAAGGTTAGGCTGTTTTTGGTGGAGAAGTTAATTTAGACTCTGTCTCTGGGTTAGTTTTCCATAGTTCTTCTTTATATATGTTGTCTTTGTTCCAACAAAGTTTTCACTTAGAGCTCAAACCCACTTCAATATTCTGTGTTGGGTTTTGAAACAATTGGATTATCGACGTTTTTCTGCAGGCTCTGGGTATCGATTTCGAACAGTTCAGGGGATGGCCTTCTCGATCATGTCTGACGAAGATGATCCTTATCCCTCTTGGGAAAAAACGAGAATGCCTATTCGTATTTTCTTCAAGGATGCCAGGTAAAGAAGCTGCTCAATATTTATCAAACTTTTTTGTTGTCTTTGTTTCGCTATGCTTTCTAGACACAGATTCAATGGGGGACTGTCTTTTAGCTTTCTGGATTATGATCCATTATGTTTGTAATAGGAAACATTCTTTCATGCGTGGTAATTCCTTTCTTGTTCTGTTTTTTGTGAACAGACATGTCTTTAAGTTGGATGAACTTGGTCGGGAAATAGCTCAGATTGCTTTGCCTGCCGCACTAGCTTTGGCAGCTGACCCTGTAGCTTCTCTGGTTGACACAGCATTCATTGGCCAAATAGGTAACTCTGCTTTTCTTTTCAAGATTTTTAGGAATCACGGATCTCTACAATTGTATGATATTGTCTACTTTAAGCATAAGCTCTCGTAACTTTGCTTTGGGCTTCCCCAAAAGGCCTCATACCAATGGACATGTATTCCTTACTTATTAACCCGTGATCAATCCCTAAATTAGCTAATATGCGACTCCCTCCCAACAATTCTCAACAAGGGTCGTGTTCATATAGCTATATCATGGATGCAGGTCCTGTGGAGCTTGCTGCCGTTGGAGTTGCTATTGCTTTATTCAATCAAGTTTCAAGAATTGCAATTTTCCCCCTTGTTAGTGTCACCACATCTTTTGTTGCTGAGGAAGATGCTATTGGAAGTGCTTGTAATGAAGCAAAGGATAATAACGATAAGGAGACAGGTTTATTCACAAATGATGAATCAAAATTGATGATCCCACACAATGGTATGCATTCCTTCATCCACTACTCAACGTTCTTCTGATTCATCATCTGATTCCATTTGTTATCCTTGACAGGGAAAACTGAAGAGAATGGAAGAAGATATATCCCATCGGCCTCTTCGGCTTTGGTTATCGGCGGTGTTCTTGGTCTCATACAAGCCATTTTCTTGATATCTGGAGCAAAACCTCTACTAAACTTCATGGGAGTCAAGTCAGTAAGTATTACCTATTGAAACTAATGCCTCAATCCTTTCTTGTACATGATTGTTGCTGCATAACATGAACGTAGGATTCGCCGATGATGACTTCTGCACAACAATACTTGACACTGCGGTCACTCGGTGCCCCGGCAGTTCTTCTCTCCTTGGCCATGCAAGGCGTCTTTCGCGGTTTTAAGGACACGAAAACTCCTTTATTTGCAACTGGTATACTTAAAACCACCTTTTCTTTTTGGAACCTCTGAATTATCCATGTTCATATTTCCCCCCTTGTTCTTCATCAGTGGCTGGAGATGCAACAAACATCATTCTAGACCCAATATTCATATTCATTTTTCGTTTAGGCGTCAGTGGTGCAGCCATTGCACACGTTATATCGCAGTAAGTGTTATGTTATACGTCGATTCCACGACTTCTCGAGCATTTTCTGGCTATTGTTTTTACATTTACTTCTTATTTTGTAGGTACCTAATAGCACTGATACTCTTTTGGAGATTAATGGGACAAGTTGATCTCTTACCTCCCAGTATCAAACATTTGCAATTTAGTCGGTTTCTGAAAAATGGTAAGAGATTTGTGTAAAGCATGCTGCAAAATCTGAAGATTTGATTAACCATGTTCATGCTTCTGTAGGCTTTCTATTATTAATGAGAGTCATTGCTGTGACGTTCTGCGTGACGCTTGCTGCGTCCCTGGCTGCACGCCAAGGATCCACATCAATGGCGGCATTTCAGGTCTGCTTGCAGGTCTGGTTGGCAATGTCTCTACTTGCCGATGGCTTAGCTGTTGCTGGGCAGGTATGTTAAAGATTTCTTTCAATCATTTGCATATGTATTCAGAACTACAAGCCTATCATGTTAAGTGTTCTTATTGTTCTTCAGACAATACTAGCAAGTGCATTTGCCCAAAACGACCATGATAAGGCAACGACTGCAGCATCACGAGTATTACAGGTTCCTATCGTTTTTTGAGAGATCTAATTACTTTTAGTAAAGGGAGTTTCTCTAATATATAGTAAACTATACTGCAGCTGGGATTGTTGCTGGGATTGGGGCTTGGAGTCTTCGTTGGAGCCGGGATGACATTCGGGGCAAAGTTATTTACAAGTGACGTCGATGTCCTCCACCTAATCGGCATAGGAACTCCAGTATGTCACCTTGTAACTCTGCCAACGCAAACTATAGGAATAAATCACTCATTTCAATATCAAACGGAAGCCAAAACTGAATTTTGTTTTAATCTGTACAGTTTATTGCTGCTATGCAACCAATCAATGCCTTGGCGTTTGTTTTTGATGGCATCAACTTTGGAGCTTCTGATTTTGCGTACTCAGCTTACTCCATGGTGAGTTCATAGTCCCCCTGTTTGCATACTCACCAATTATACAAAAAACAACTATGGTGGCTGGTTCTTGTCCTAAAATCTAGTCATGGTCGGGATCTTACAAATGTTGAGGATTGTTGGGAGATGAGTCCCACATCGGCTAAATAAGGGGTTGATCATGGGTTTATAAGTAAGGAACACTATCTTCATTTGTATGAGGCCGTTTGAGGAAACCAAAAGTAAAGCCACGAGAGCTTATGCTCAAAGTAGACAATATCATACAATTGTGGAGGTTCGTGGTTTCTGACATGGTATCAGAGCCATGCCCTTAACTTAGCCATGTCAATAGAATCCTCAAGTGTCGAACAAAGGGTGTACTTTGTTCGAAGGCTTCAGAATAAGAGTCGAGTCTCGATTAAGGGGAGGCTATTCGAGGGCTCCATAGGTCTTAGGGGAGGCTGTTCGAGGGCTCCATATGTCTTAGGGGAGGCTGTTCGAGGGCTCCATAGGTCTTAGGGGAGGCTTTATCGTGTACTTTGTTCGAGGGAAGGATGATTGAGGATTGTTGGGAGAGGAGTGTCACATCGGCTAATTAAGGGGTTGATCATGAGTTTATAAGTAAGGAACAGTATCTTTATTGGTATGAGACCTTTTGGGTAAACCAAAAGCAAAGCCACGAAAGCTTATGCTCAAAGTGAACAATATCATACCAATATGGAGGTCCGTGGTTCCTAACAACAATGATACGTATAGTAACTCGAGCCTAAAATGAACAAGATCGTAGCCTAAAATGAATTCTAGATGAGCTAAATGATGAGCTTTAATCATCTAACCATCCAAACTTACATTCCTTCTCTCCAGGTTCTGGTGGCTATTATCAGCATCTTCTGTTTGTTCATTCTCTCCTCAACTCAAGGATTCATCGGTATCTGGGTCGCCTTAACCATCTACATGAGCTTACGAACACTAGCCGGATTCTGGAGGTACTTCCATCTACACTCTATCTATCCGAACTCGTGTCATCCAACACTCGTTAACGAACGATCACAAATCAGCTACTATATTGCGGTGTTCATTACAAAAATCTTATGTTTTTGAACTGCACTGACCTTCGCTTACAGGGTCGGCACGGGAACAGGACCTTGGTATTTCCTCCAAAGCTAGATTCCAAGTCGTTTGTAGGACATGGATAAGCTGTTGGATGATGTTTTTTTCTTCCATTTATGTCCATACCTTCATATATAGGCTGGATTGCATCAGGACATTCAGCTTAAAACCTTAAAAAGTTGAAGCTTAGAGACCATGTACAGAAAAGACAAGAGAAGACAGCATGTTCTTGAACAAGAAGACAAGAAAACATAGAAGATTTGTACGAGTGTACGAGTGTTTTGTAGCTGTAAATCACAGTGTTCAGTTCAGTGTTACAAGATTTTATGCAGGTTGTTTGTTATATGCAATCCTCTCCTTTGAAACTATTTGTTTAAAAATGTATTTTTTTTTATTATTGGTAAAAA

mRNA sequence

ATTAGCTTATAATTGTTAATAAATGCAAGGAAATTATAGGTAAATTACCGGCGGTGAAATTCGGTATCGAACACTGGCCGCTTTTCCTTAGCGGGAAGGACACTTGAGGCCTCTTCCCTTTGCCGCTTGCCTTTTGCCCGCCAACGCCAACGCCAACGCCAACGAATGCCTCACTTCCATTTGCTTCTCTTCGTCAACTGACCAACAAACTCGTCCTCTTCGCCTCCGACCCTCACAGGCTCCTACTAATCAAACCAATTTTTCAATACCTCAATTGGCAACTGGCTTCAGACAAAGACACCCTCTTCCCTCTTTCTTCCGTGCTTCCAATTTGAATTGGGGTTTCTTCCACGCCGTCGGAGTTGAGACTCTGTCTCTGGGCTCTGGGTATCGATTTCGAACAGTTCAGGGGATGGCCTTCTCGATCATGTCTGACGAAGATGATCCTTATCCCTCTTGGGAAAAAACGAGAATGCCTATTCGTATTTTCTTCAAGGATGCCAGACATGTCTTTAAGTTGGATGAACTTGGTCGGGAAATAGCTCAGATTGCTTTGCCTGCCGCACTAGCTTTGGCAGCTGACCCTGTAGCTTCTCTGTTTCAAGAATTGCAATTTTCCCCCTTTGTCACCACATCTTTTGTTGCTGAGGAAGATGCTATTGGAAGTGCTTGTAATGAAGCAAAGGATAATAACGATAAGGAGACAGGTTTATTCACAAATGATGAATCAAAATTGATGATCCCACACAATGGGAAAACTGAAGAGAATGGAAGAAGATATATCCCATCGGCCTCTTCGGCTTTGGTTATCGGCGGTGTTCTTGGTCTCATACAAGCCATTTTCTTGATATCTGGAGCAAAACCTCTACTAAACTTCATGGGAGTCAAGTCAGATTCGCCGATGATGACTTCTGCACAACAATACTTGACACTGCGGTCACTCGGTGCCCCGGCAGTTCTTCTCTCCTTGGCCATGCAAGGCGTCTTTCGCGGTTTTAAGGACACGAAAACTCCTTTATTTGCAACTGTGGCTGGAGATGCAACAAACATCATTCTAGACCCAATATTCATATTCATTTTTCGTTTAGGCGTCAGTGGTGCAGCCATTGCACACGTTATATCGCAGTACCTAATAGCACTGATACTCTTTTGGAGATTAATGGGACAAGTTGATCTCTTACCTCCCAGTATCAAACATTTGCAATTTAGTCGGTTTCTGAAAAATGGCTTTCTATTATTAATGAGAGTCATTGCTGTGACGTTCTGCGTGACGCTTGCTGCGTCCCTGGCTGCACGCCAAGGATCCACATCAATGGCGGCATTTCAGGTCTGCTTGCAGGTCTGGTTGGCAATGTCTCTACTTGCCGATGGCTTAGCTGTTGCTGGGCAGACAATACTAGCAAGTGCATTTGCCCAAAACGACCATGATAAGGCAACGACTGCAGCATCACGAGTATTACAGCTGGGATTGTTGCTGGGATTGGGGCTTGGAGTCTTCGTTGGAGCCGGGATGACATTCGGGGCAAAGTTATTTACAAGTGACGTCGATGTCCTCCACCTAATCGGCATAGGAACTCCATTTATTGCTGCTATGCAACCAATCAATGCCTTGGCGTTTGTTTTTGATGGCATCAACTTTGGAGCTTCTGATTTTGCGTACTCAGCTTACTCCATGGTTCTGGTGGCTATTATCAGCATCTTCTGTTTGTTCATTCTCTCCTCAACTCAAGGATTCATCGGTATCTGGGTCGCCTTAACCATCTACATGAGCTTACGAACACTAGCCGGATTCTGGAGGGTCGGCACGGGAACAGGACCTTGGTATTTCCTCCAAAGCTAGATTCCAAGTCGTTTGTAGGACATGGATAAGCTGTTGGATGATGTTTTTTTCTTCCATTTATGTCCATACCTTCATATATAGGCTGGATTGCATCAGGACATTCAGCTTAAAACCTTAAAAAGTTGAAGCTTAGAGACCATGTACAGAAAAGACAAGAGAAGACAGCATGTTCTTGAACAAGAAGACAAGAAAACATAGAAGATTTGTACGAGTGTACGAGTGTTTTGTAGCTGTAAATCACAGTGTTCAGTTCAGTGTTACAAGATTTTATGCAGGTTGTTTGTTATATGCAATCCTCTCCTTTGAAACTATTTGTTTAAAAATGTATTTTTTTTTATTATTGGTAAAAA

Coding sequence (CDS)

ATGGCCTTCTCGATCATGTCTGACGAAGATGATCCTTATCCCTCTTGGGAAAAAACGAGAATGCCTATTCGTATTTTCTTCAAGGATGCCAGACATGTCTTTAAGTTGGATGAACTTGGTCGGGAAATAGCTCAGATTGCTTTGCCTGCCGCACTAGCTTTGGCAGCTGACCCTGTAGCTTCTCTGTTTCAAGAATTGCAATTTTCCCCCTTTGTCACCACATCTTTTGTTGCTGAGGAAGATGCTATTGGAAGTGCTTGTAATGAAGCAAAGGATAATAACGATAAGGAGACAGGTTTATTCACAAATGATGAATCAAAATTGATGATCCCACACAATGGGAAAACTGAAGAGAATGGAAGAAGATATATCCCATCGGCCTCTTCGGCTTTGGTTATCGGCGGTGTTCTTGGTCTCATACAAGCCATTTTCTTGATATCTGGAGCAAAACCTCTACTAAACTTCATGGGAGTCAAGTCAGATTCGCCGATGATGACTTCTGCACAACAATACTTGACACTGCGGTCACTCGGTGCCCCGGCAGTTCTTCTCTCCTTGGCCATGCAAGGCGTCTTTCGCGGTTTTAAGGACACGAAAACTCCTTTATTTGCAACTGTGGCTGGAGATGCAACAAACATCATTCTAGACCCAATATTCATATTCATTTTTCGTTTAGGCGTCAGTGGTGCAGCCATTGCACACGTTATATCGCAGTACCTAATAGCACTGATACTCTTTTGGAGATTAATGGGACAAGTTGATCTCTTACCTCCCAGTATCAAACATTTGCAATTTAGTCGGTTTCTGAAAAATGGCTTTCTATTATTAATGAGAGTCATTGCTGTGACGTTCTGCGTGACGCTTGCTGCGTCCCTGGCTGCACGCCAAGGATCCACATCAATGGCGGCATTTCAGGTCTGCTTGCAGGTCTGGTTGGCAATGTCTCTACTTGCCGATGGCTTAGCTGTTGCTGGGCAGACAATACTAGCAAGTGCATTTGCCCAAAACGACCATGATAAGGCAACGACTGCAGCATCACGAGTATTACAGCTGGGATTGTTGCTGGGATTGGGGCTTGGAGTCTTCGTTGGAGCCGGGATGACATTCGGGGCAAAGTTATTTACAAGTGACGTCGATGTCCTCCACCTAATCGGCATAGGAACTCCATTTATTGCTGCTATGCAACCAATCAATGCCTTGGCGTTTGTTTTTGATGGCATCAACTTTGGAGCTTCTGATTTTGCGTACTCAGCTTACTCCATGGTTCTGGTGGCTATTATCAGCATCTTCTGTTTGTTCATTCTCTCCTCAACTCAAGGATTCATCGGTATCTGGGTCGCCTTAACCATCTACATGAGCTTACGAACACTAGCCGGATTCTGGAGGGTCGGCACGGGAACAGGACCTTGGTATTTCCTCCAAAGCTAG

Protein sequence

MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLFQELQFSPFVTTSFVAEEDAIGSACNEAKDNNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGTGTGPWYFLQS
Homology
BLAST of Cp4.1LG05g05060 vs. ExPASy Swiss-Prot
Match: Q9SYD6 (Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2)

HSP 1 Score: 567.8 bits (1462), Expect = 1.2e-160
Identity = 323/503 (64.21%), Postives = 367/503 (72.96%), Query Frame = 0

Query: 20  RMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLFQEL---QFSPF----- 79
           R P+ IFF D R V K DELG EIA+IALPAALAL ADP+ASL       Q  P      
Sbjct: 13  RNPLYIFFSDFRSVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAV 72

Query: 80  --------------------VTTSFVAEEDAIGSACNEAKDNND-KETGLFTNDESKL-M 139
                               +TTSFVAEEDA  S  +  +D+ +  E G+    E  + +
Sbjct: 73  GVSIALFNQVSRIAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIEL 132

Query: 140 IPHNGKTEENG-----------------RRYIPSASSALVIGGVLGLIQAIFLISGAKPL 199
           IP   K   +                  +R IPSASSAL+IGGVLGL QA+FLIS AKPL
Sbjct: 133 IPEKHKDSLSDEFKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPL 192

Query: 200 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 259
           L+FMGVK DSPMM  +Q+YL+LRSLGAPAVLLSLA QGVFRGFKDT TPLFATV GD TN
Sbjct: 193 LSFMGVKHDSPMMRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTN 252

Query: 260 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 319
           IILDPIFIF+FRLGV+GAA AHVISQYL+  IL W+LMGQVD+   S KHLQF RF+KNG
Sbjct: 253 IILDPIFIFVFRLGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNG 312

Query: 320 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 379
           FLLLMRVIAVTFCVTL+ASLAAR+GSTSMAAFQVCLQVWLA SLLADG AVAGQ ILASA
Sbjct: 313 FLLLMRVIAVTFCVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASA 372

Query: 380 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 439
           FA+ D+ +A   ASRVLQLGL+LG  L V +GAG+ FGA++FT D  VLHLI IG PF+A
Sbjct: 373 FAKKDYKRAAATASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVA 432

Query: 440 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 476
             QPINALAFVFDG+NFGASDF Y+A S+V+VAI+SI CL  LSST GFIG+W  LTIYM
Sbjct: 433 GTQPINALAFVFDGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYM 492

BLAST of Cp4.1LG05g05060 vs. ExPASy Swiss-Prot
Match: Q9SFB0 (Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.8e-137
Identity = 284/507 (56.02%), Postives = 346/507 (68.24%), Query Frame = 0

Query: 18  KTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLF-------------- 77
           K  +P  + FKD RHVF  D  GREI  IA PAALALAADP+ASL               
Sbjct: 12  KKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLA 71

Query: 78  ------------QELQFSPFV--TTSFVAEEDAIGSACNEAKDNN-----------DKET 137
                         +   P V  TTSFVAEED +     EA   N             E 
Sbjct: 72  AVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEK 131

Query: 138 GL---FTNDESKLMIPHNGKTEENG--------RRYIPSASSALVIGGVLGLIQAIFLIS 197
           G+    +ND ++   P    T+ N         +R I +AS+A+++G +LGL+QAIFLI 
Sbjct: 132 GISSPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIF 191

Query: 198 GAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVA 257
            +K LL  MGVK +SPM++ A +YL++R+LGAPA+LLSLAMQG+FRGFKDTKTPLFATV 
Sbjct: 192 SSKLLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVV 251

Query: 258 GDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSR 317
            D  NI+LDPIFIF+ RLG+ GAAIAHVISQY + LILF  L  +V+L+PP+   LQF R
Sbjct: 252 ADVINIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGR 311

Query: 318 FLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQT 377
           FLKNG LLL R IAVTFC TLAA++AAR G+T MAAFQ+CLQVWL  SLL DGLAVAGQ 
Sbjct: 312 FLKNGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQA 371

Query: 378 ILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIG 437
           ILA +FA+ D++K T  ASRVLQ+G +LGLGL VFVG G+ FGA +F+ D  V+HL+ IG
Sbjct: 372 ILACSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIG 431

Query: 438 TPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVA 475
            PFIAA QPIN+LAFV DG+NFGASDFAY+AYSMV VA ISI  +  ++ T GFIGIW+A
Sbjct: 432 IPFIAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIA 491

BLAST of Cp4.1LG05g05060 vs. ExPASy Swiss-Prot
Match: Q84K71 (Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX44 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.7e-90
Identity = 205/468 (43.80%), Postives = 282/468 (60.26%), Query Frame = 0

Query: 36  LDELGREIAQIALPAALALAADPVASLFQE---------------LQFSPF--------- 95
           + ++G EI  IALPAALALAADP+ SL                  +  S F         
Sbjct: 73  IGKIGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNV 132

Query: 96  ----VTTSFVAEEDAIGSACNEAKDNNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSA 155
               VTTSFVAEE AI      AKD+ND                    + E  ++ +PS 
Sbjct: 133 PLLNVTTSFVAEEQAIA-----AKDDND--------------------SIETSKKVLPSV 192

Query: 156 SSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLA 215
           S++LV+   +G+ +AI L  G+  L++ M +  DSPM   A+Q+L LR+ GAP ++++LA
Sbjct: 193 STSLVLAAGVGIAEAIALSLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALA 252

Query: 216 MQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFW 275
            QG FRGFKDT TPL+A VAG+  N +LDPI IF+   G+SGAA A VIS+YLIA IL W
Sbjct: 253 AQGAFRGFKDTTTPLYAVVAGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLW 312

Query: 276 RLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVC 335
           +L   V LL P IK  + +++LK+G LL+ R +A+    TLA SLAA+ G T MA  Q+ 
Sbjct: 313 KLNENVVLLSPQIKVGRANQYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIV 372

Query: 336 LQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGM 395
           L++WLA+SLL D LA+A Q++LA+ ++Q ++ +A      VLQ+GL  G GL   +    
Sbjct: 373 LEIWLAVSLLTDALAIAAQSLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITF 432

Query: 396 TFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAII 455
              + LFT+D +VL +   GT F+A  QP+NALAFV DG+ +G SDF ++AYSMV+V  I
Sbjct: 433 EPFSSLFTTDSEVLKIALSGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFI 492

Query: 456 SIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGTGTGPWYFLQS 476
           S   + + + T G  GIW  L ++M+LR +AG WR+GT TGPW  L S
Sbjct: 493 SSLFMLVAAPTFGLAGIWTGLFLFMALRLVAGAWRLGTRTGPWKMLWS 515

BLAST of Cp4.1LG05g05060 vs. ExPASy Swiss-Prot
Match: Q9SVE7 (Protein DETOXIFICATION 45, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX45 PE=2 SV=2)

HSP 1 Score: 297.0 bits (759), Expect = 3.9e-79
Identity = 188/498 (37.75%), Postives = 290/498 (58.23%), Query Frame = 0

Query: 6   MSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLFQE 65
           + +EDD   S +K      +    AR V    ++ RE+  ++LPA    A DP+  L + 
Sbjct: 76  LGEEDDSCSSLDKLPEVNGVHTGVARPV----DIKRELVMLSLPAIAGQAIDPLTLLMET 135

Query: 66  LQFSPF----------------------------VTTSFVAEEDAIGSACNEAKDNNDKE 125
                                             V TSFVAE+ A  +A + A +++  +
Sbjct: 136 AYIGRLGSVELGSAGVSMAIFNTISKLFNIPLLSVATSFVAEDIAKIAAQDLASEDSQSD 195

Query: 126 TGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPLLNFMG 185
                       IP  G  E   R+ + S S+ALV+   +G+ +A+ L   + P L  MG
Sbjct: 196 ------------IPSQGLPE---RKQLSSVSTALVLAIGIGIFEALALSLASGPFLRLMG 255

Query: 186 VKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATNIILDP 245
           ++S S M   A+Q+L LR+LGAPA ++SLA+QG+FRGFKDTKTP++    G+   + L P
Sbjct: 256 IQSMSEMFIPARQFLVLRALGAPAYVVSLALQGIFRGFKDTKTPVYCLGIGNFLAVFLFP 315

Query: 246 IFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNGFLLLM 305
           +FI+ FR+GV+GAAI+ VISQY +A+++   L  +V LLPP I  L+F  +LK+G  +L 
Sbjct: 316 LFIYKFRMGVAGAAISSVISQYTVAILMLILLNKRVILLPPKIGSLKFGDYLKSGGFVLG 375

Query: 306 RVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQND 365
           R ++V   +T+A S+AARQG  +MAA Q+C+QVWLA+SLL D LA +GQ ++AS+ ++ D
Sbjct: 376 RTLSVLVTMTVATSMAARQGVFAMAAHQICMQVWLAVSLLTDALASSGQALIASSASKRD 435

Query: 366 HDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIAAMQPI 425
            +      + VL++G++ G+ L + +G   +  A LF+ D +VL ++  G  F+AA QPI
Sbjct: 436 FEGVKEVTTFVLKIGVVTGIALAIVLGMSFSSIAGLFSKDPEVLRIVRKGVLFVAATQPI 495

Query: 426 NALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTL 476
            ALAF+FDG+++G SDF Y+A SM++V  IS   +    +  G  G+WV L+++M LR +
Sbjct: 496 TALAFIFDGLHYGMSDFPYAACSMMVVGGISSAFMLYAPAGLGLSGVWVGLSMFMGLRMV 554

BLAST of Cp4.1LG05g05060 vs. ExPASy Swiss-Prot
Match: Q2FJS9 (Multidrug export protein MepA OS=Staphylococcus aureus (strain USA300) OX=367830 GN=mepA PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-09
Identity = 85/340 (25.00%), Postives = 143/340 (42.06%), Query Frame = 0

Query: 128 SSALVIGGV-LGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSL 187
           SS  + GG+ LGLI  +  +  +  +   +G + ++  +TS   YL +  L AP V+L  
Sbjct: 96  SSFSIYGGIALGLIVILVTLPFSDQIAAILGARGETLALTS--NYLKVMFLSAPFVILFF 155

Query: 188 AMQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILF 247
            ++   R        +   +A    NIILDPI IF F L V GAA+   IS    AL   
Sbjct: 156 ILEQFARAIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFI 215

Query: 248 WRLMGQVDLLPPSIKHLQ-----FSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSM 307
              M   D++  +IK  +      S   K G    +  I + F   +     A  G+ ++
Sbjct: 216 IYFMKNSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAI 275

Query: 308 AAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGV 367
           A++ +  ++     L+  GL      ++A  F  N         + ++ +G++      V
Sbjct: 276 ASYGISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVI----FVV 335

Query: 368 FVGAGMTFG---AKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSA 427
            + A  T G     LFT+D  ++ +         A   +N + F+F G+         + 
Sbjct: 336 CMSAVFTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGAT 395

Query: 428 YSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTLA 459
              +L   I I  LFI+++  G  G+  +L I  SL  LA
Sbjct: 396 IMAILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALA 429

BLAST of Cp4.1LG05g05060 vs. NCBI nr
Match: XP_023532550.1 (protein DETOXIFICATION 42-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 866 bits (2237), Expect = 3.05e-315
Identity = 468/503 (93.04%), Postives = 468/503 (93.04%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA
Sbjct: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEEDAIGSACNEAKD
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKD 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180
           NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL
Sbjct: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180

Query: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240
           LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN
Sbjct: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240

Query: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300
           IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG
Sbjct: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300

Query: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360
           FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA
Sbjct: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360

Query: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420
           FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA
Sbjct: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420

Query: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 475
           AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM
Sbjct: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 480

BLAST of Cp4.1LG05g05060 vs. NCBI nr
Match: KAG7035862.1 (Protein DETOXIFICATION 42 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863 bits (2229), Expect = 5.96e-314
Identity = 466/503 (92.64%), Postives = 468/503 (93.04%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMS+EDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA
Sbjct: 1   MAFSIMSEEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEEDAIGSACNEAKD
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKD 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180
           NNDKETGLFTN+ESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL
Sbjct: 121 NNDKETGLFTNEESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180

Query: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240
           LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN
Sbjct: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240

Query: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300
           IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG
Sbjct: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300

Query: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360
           FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA
Sbjct: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360

Query: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420
           FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA
Sbjct: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420

Query: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 475
           AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM
Sbjct: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 480

BLAST of Cp4.1LG05g05060 vs. NCBI nr
Match: XP_022957625.1 (protein DETOXIFICATION 42-like [Cucurbita moschata])

HSP 1 Score: 862 bits (2226), Expect = 1.70e-313
Identity = 465/503 (92.45%), Postives = 468/503 (93.04%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMS+EDDPYPSWEKTRMPIRIFFKDARHVFKLD+LGREIAQIALPAALALAADPVA
Sbjct: 1   MAFSIMSEEDDPYPSWEKTRMPIRIFFKDARHVFKLDDLGREIAQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEEDAIGSACNEAKD
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKD 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180
           NNDKETGLFTN+ESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL
Sbjct: 121 NNDKETGLFTNEESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180

Query: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240
           LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN
Sbjct: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240

Query: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300
           IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG
Sbjct: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300

Query: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360
           FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA
Sbjct: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360

Query: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420
           FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA
Sbjct: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420

Query: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 475
           AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM
Sbjct: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 480

BLAST of Cp4.1LG05g05060 vs. NCBI nr
Match: XP_022995068.1 (protein DETOXIFICATION 42-like [Cucurbita maxima])

HSP 1 Score: 861 bits (2224), Expect = 3.41e-313
Identity = 465/503 (92.45%), Postives = 467/503 (92.84%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREI+QIALPAALALAADPVA
Sbjct: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREISQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEEDAIGSACNEAKD
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKD 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180
           NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL
Sbjct: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180

Query: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240
           LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN
Sbjct: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240

Query: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300
           IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRL+GQVDLLPPSIKHLQFSRFLKNG
Sbjct: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLIGQVDLLPPSIKHLQFSRFLKNG 300

Query: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360
           FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA
Sbjct: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360

Query: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420
           FAQNDHDKA TAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA
Sbjct: 361 FAQNDHDKAMTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420

Query: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 475
           AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM
Sbjct: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 480

BLAST of Cp4.1LG05g05060 vs. NCBI nr
Match: KAG6605914.1 (Protein DETOXIFICATION 42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 821 bits (2121), Expect = 7.85e-298
Identity = 447/483 (92.55%), Postives = 448/483 (92.75%), Query Frame = 0

Query: 21  MPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLFQEL---QFSPF------ 80
           MPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASL       Q  P       
Sbjct: 1   MPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLVDTAFIGQIGPVELAAVG 60

Query: 81  -------------------VTTSFVAEEDAIGSACNEAKDNNDKETGLFTNDESKLMIPH 140
                              VTTSFVAEEDAIGSACNEAKDNNDKETGLFTN+ESKLMIPH
Sbjct: 61  VAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKDNNDKETGLFTNEESKLMIPH 120

Query: 141 NGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYL 200
           NGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYL
Sbjct: 121 NGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYL 180

Query: 201 TLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAI 260
           TLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAI
Sbjct: 181 TLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAI 240

Query: 261 AHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASL 320
           AHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASL
Sbjct: 241 AHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASL 300

Query: 321 AARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLG 380
           AARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLG
Sbjct: 301 AARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLG 360

Query: 381 LLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGAS 440
           LLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGAS
Sbjct: 361 LLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGAS 420

Query: 441 DFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGTGTGPWYF 475
           DFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGTGTGPWYF
Sbjct: 421 DFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGTGTGPWYF 480

BLAST of Cp4.1LG05g05060 vs. ExPASy TrEMBL
Match: A0A6J1H128 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459102 PE=3 SV=1)

HSP 1 Score: 862 bits (2226), Expect = 8.21e-314
Identity = 465/503 (92.45%), Postives = 468/503 (93.04%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMS+EDDPYPSWEKTRMPIRIFFKDARHVFKLD+LGREIAQIALPAALALAADPVA
Sbjct: 1   MAFSIMSEEDDPYPSWEKTRMPIRIFFKDARHVFKLDDLGREIAQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEEDAIGSACNEAKD
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKD 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180
           NNDKETGLFTN+ESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL
Sbjct: 121 NNDKETGLFTNEESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180

Query: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240
           LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN
Sbjct: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240

Query: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300
           IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG
Sbjct: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300

Query: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360
           FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA
Sbjct: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360

Query: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420
           FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA
Sbjct: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420

Query: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 475
           AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM
Sbjct: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 480

BLAST of Cp4.1LG05g05060 vs. ExPASy TrEMBL
Match: A0A6J1K6V0 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111490726 PE=3 SV=1)

HSP 1 Score: 861 bits (2224), Expect = 1.65e-313
Identity = 465/503 (92.45%), Postives = 467/503 (92.84%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREI+QIALPAALALAADPVA
Sbjct: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREISQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEEDAIGSACNEAKD
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDAIGSACNEAKD 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180
           NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL
Sbjct: 121 NNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSASSALVIGGVLGLIQAIFLISGAKPL 180

Query: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240
           LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN
Sbjct: 181 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 240

Query: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 300
           IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRL+GQVDLLPPSIKHLQFSRFLKNG
Sbjct: 241 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLIGQVDLLPPSIKHLQFSRFLKNG 300

Query: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360
           FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA
Sbjct: 301 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 360

Query: 361 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420
           FAQNDHDKA TAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA
Sbjct: 361 FAQNDHDKAMTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 420

Query: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 475
           AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM
Sbjct: 421 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 480

BLAST of Cp4.1LG05g05060 vs. ExPASy TrEMBL
Match: A0A1S3BM07 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103491512 PE=3 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 4.60e-276
Identity = 423/521 (81.19%), Postives = 441/521 (84.64%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMS+EDDPYP+W+KT+ PIRIFFKDAR+VFKLDELGREIA+IALPAALALAADPVA
Sbjct: 1   MAFSIMSEEDDPYPAWDKTKTPIRIFFKDARNVFKLDELGREIARIALPAALALAADPVA 60

Query: 61  SLFQ--------------------------ELQFSPFV--TTSFVAEEDAIGSACNEAKD 120
           SL                             +   P V  TTSFVAEED IGS   EA+D
Sbjct: 61  SLVDTAFIGQIGSVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDTIGSVSIEAED 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTE------------------ENGRRYIPSASSALVIG 180
           NND ETG FTNDE   MIP NGK E                  ENGRRYIPSASSALVIG
Sbjct: 121 NNDTETGFFTNDEKSSMIPQNGKGEDAHHSRKPLDTTFENGKVENGRRYIPSASSALVIG 180

Query: 181 GVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRG 240
           GVLGLIQAIFLISGA+PLLNFMGVKSDS MMT AQQYLTLRSLGAPAVLLSLA+QGVFRG
Sbjct: 181 GVLGLIQAIFLISGARPLLNFMGVKSDSLMMTPAQQYLTLRSLGAPAVLLSLAIQGVFRG 240

Query: 241 FKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVD 300
           FKDTKTPL+ATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVD
Sbjct: 241 FKDTKTPLYATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVD 300

Query: 301 LLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAM 360
           LLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWL  
Sbjct: 301 LLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLTT 360

Query: 361 SLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLF 420
           SLLADGLAVAGQ ILA+AFAQNDHDKAT AASRVLQLGL LGL L VF+G GMTFGAKLF
Sbjct: 361 SLLADGLAVAGQAILATAFAQNDHDKATAAASRVLQLGLFLGLMLSVFLGVGMTFGAKLF 420

Query: 421 TSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFI 475
           TSDVDVL  IGIG PF+AA QPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFI
Sbjct: 421 TSDVDVLRFIGIGIPFVAATQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFI 480

BLAST of Cp4.1LG05g05060 vs. ExPASy TrEMBL
Match: A0A5D3BAV3 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001070 PE=3 SV=1)

HSP 1 Score: 767 bits (1980), Expect = 4.60e-276
Identity = 423/521 (81.19%), Postives = 441/521 (84.64%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMS+EDDPYP+W+KT+ PIRIFFKDAR+VFKLDELGREIA+IALPAALALAADPVA
Sbjct: 1   MAFSIMSEEDDPYPAWDKTKTPIRIFFKDARNVFKLDELGREIARIALPAALALAADPVA 60

Query: 61  SLFQ--------------------------ELQFSPFV--TTSFVAEEDAIGSACNEAKD 120
           SL                             +   P V  TTSFVAEED IGS   EA+D
Sbjct: 61  SLVDTAFIGQIGSVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDTIGSVSIEAED 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTE------------------ENGRRYIPSASSALVIG 180
           NND ETG FTNDE   MIP NGK E                  ENGRRYIPSASSALVIG
Sbjct: 121 NNDTETGFFTNDEKSSMIPQNGKGEDAHHSRKPLDTTFENGKVENGRRYIPSASSALVIG 180

Query: 181 GVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRG 240
           GVLGLIQAIFLISGA+PLLNFMGVKSDS MMT AQQYLTLRSLGAPAVLLSLA+QGVFRG
Sbjct: 181 GVLGLIQAIFLISGARPLLNFMGVKSDSLMMTPAQQYLTLRSLGAPAVLLSLAIQGVFRG 240

Query: 241 FKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVD 300
           FKDTKTPL+ATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVD
Sbjct: 241 FKDTKTPLYATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVD 300

Query: 301 LLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAM 360
           LLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWL  
Sbjct: 301 LLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLTT 360

Query: 361 SLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLF 420
           SLLADGLAVAGQ ILA+AFAQNDHDKAT AASRVLQLGL LGL L VF+G GMTFGAKLF
Sbjct: 361 SLLADGLAVAGQAILATAFAQNDHDKATAAASRVLQLGLFLGLMLSVFLGVGMTFGAKLF 420

Query: 421 TSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFI 475
           TSDVDVL  IGIG PF+AA QPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFI
Sbjct: 421 TSDVDVLRFIGIGIPFVAATQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFI 480

BLAST of Cp4.1LG05g05060 vs. ExPASy TrEMBL
Match: A0A6J1D660 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111017410 PE=3 SV=1)

HSP 1 Score: 762 bits (1967), Expect = 4.22e-274
Identity = 418/518 (80.69%), Postives = 440/518 (84.94%), Query Frame = 0

Query: 1   MAFSIMSDEDDPYPSWEKTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVA 60
           MAFSIMS+EDDPYPSW+KTR PIRIFFKDAR+VFKLDELGREIAQIALPAALALAADPVA
Sbjct: 1   MAFSIMSEEDDPYPSWDKTRTPIRIFFKDARNVFKLDELGREIAQIALPAALALAADPVA 60

Query: 61  SLFQEL---QFSPF-------------------------VTTSFVAEEDAIGSACNEAKD 120
           SL       Q  P                          VTTSFVAEED I S  N+ + 
Sbjct: 61  SLVDTAFIGQIGPVELAAVGVAIALFNQVSRIAIFPLVSVTTSFVAEEDTIRSVYNQVEG 120

Query: 121 NNDKETGLFTNDESKLMIPHNGKTE-----------------ENGRRYIPSASSALVIGG 180
           N+D ETG +TNDE KLMIP NGK E                 EN RRYIPSASSALVIGG
Sbjct: 121 NDDMETGSYTNDEKKLMIPQNGKGENTHHSNSVDRRFDSGRGENERRYIPSASSALVIGG 180

Query: 181 VLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGF 240
           VLGLIQAIFLISGAKPLLNFMGVKSDS MMT A QYLTLR+LGAPAVLLSLAMQGVFRGF
Sbjct: 181 VLGLIQAIFLISGAKPLLNFMGVKSDSLMMTPALQYLTLRALGAPAVLLSLAMQGVFRGF 240

Query: 241 KDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDL 300
           KDTKTPL+ATVAGDATNIILDPIFIFIF LGVSGAAIAHV+SQYLIALILFWRLMGQV+L
Sbjct: 241 KDTKTPLYATVAGDATNIILDPIFIFIFHLGVSGAAIAHVMSQYLIALILFWRLMGQVNL 300

Query: 301 LPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMS 360
           LPPSIKHLQFSRFLKNGFLLLMRV+AVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMS
Sbjct: 301 LPPSIKHLQFSRFLKNGFLLLMRVVAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMS 360

Query: 361 LLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFT 420
           LLADGLAVAGQ ILASAFAQNDH+KAT AASRVLQLGL LGL L VF+G G++FGAKLFT
Sbjct: 361 LLADGLAVAGQAILASAFAQNDHNKATAAASRVLQLGLFLGLALAVFLGVGLSFGAKLFT 420

Query: 421 SDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFIL 473
           +DV+VLHLIGIG PF+A  QPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFIL
Sbjct: 421 NDVNVLHLIGIGIPFVAVTQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFIL 480

BLAST of Cp4.1LG05g05060 vs. TAIR 10
Match: AT1G51340.2 (MATE efflux family protein )

HSP 1 Score: 567.8 bits (1462), Expect = 8.4e-162
Identity = 323/503 (64.21%), Postives = 367/503 (72.96%), Query Frame = 0

Query: 20  RMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLFQEL---QFSPF----- 79
           R P+ IFF D R V K DELG EIA+IALPAALAL ADP+ASL       Q  P      
Sbjct: 13  RNPLYIFFSDFRSVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAV 72

Query: 80  --------------------VTTSFVAEEDAIGSACNEAKDNND-KETGLFTNDESKL-M 139
                               +TTSFVAEEDA  S  +  +D+ +  E G+    E  + +
Sbjct: 73  GVSIALFNQVSRIAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIEL 132

Query: 140 IPHNGKTEENG-----------------RRYIPSASSALVIGGVLGLIQAIFLISGAKPL 199
           IP   K   +                  +R IPSASSAL+IGGVLGL QA+FLIS AKPL
Sbjct: 133 IPEKHKDSLSDEFKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPL 192

Query: 200 LNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATN 259
           L+FMGVK DSPMM  +Q+YL+LRSLGAPAVLLSLA QGVFRGFKDT TPLFATV GD TN
Sbjct: 193 LSFMGVKHDSPMMRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTN 252

Query: 260 IILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNG 319
           IILDPIFIF+FRLGV+GAA AHVISQYL+  IL W+LMGQVD+   S KHLQF RF+KNG
Sbjct: 253 IILDPIFIFVFRLGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNG 312

Query: 320 FLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASA 379
           FLLLMRVIAVTFCVTL+ASLAAR+GSTSMAAFQVCLQVWLA SLLADG AVAGQ ILASA
Sbjct: 313 FLLLMRVIAVTFCVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASA 372

Query: 380 FAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIA 439
           FA+ D+ +A   ASRVLQLGL+LG  L V +GAG+ FGA++FT D  VLHLI IG PF+A
Sbjct: 373 FAKKDYKRAAATASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVA 432

Query: 440 AMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYM 476
             QPINALAFVFDG+NFGASDF Y+A S+V+VAI+SI CL  LSST GFIG+W  LTIYM
Sbjct: 433 GTQPINALAFVFDGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYM 492

BLAST of Cp4.1LG05g05060 vs. TAIR 10
Match: AT1G51340.1 (MATE efflux family protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 316/490 (64.49%), Postives = 359/490 (73.27%), Query Frame = 0

Query: 33  VFKLDELGREIAQIALPAALALAADPVASLFQEL---QFSPF------------------ 92
           V K DELG EIA+IALPAALAL ADP+ASL       Q  P                   
Sbjct: 20  VLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSRI 79

Query: 93  -------VTTSFVAEEDAIGSACNEAKDNND-KETGLFTNDESKL-MIPHNGKTEENG-- 152
                  +TTSFVAEEDA  S  +  +D+ +  E G+    E  + +IP   K   +   
Sbjct: 80  AIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDEF 139

Query: 153 ---------------RRYIPSASSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMM 212
                          +R IPSASSAL+IGGVLGL QA+FLIS AKPLL+FMGVK DSPMM
Sbjct: 140 KTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPMM 199

Query: 213 TSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRL 272
             +Q+YL+LRSLGAPAVLLSLA QGVFRGFKDT TPLFATV GD TNIILDPIFIF+FRL
Sbjct: 200 RPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFRL 259

Query: 273 GVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFC 332
           GV+GAA AHVISQYL+  IL W+LMGQVD+   S KHLQF RF+KNGFLLLMRVIAVTFC
Sbjct: 260 GVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTFC 319

Query: 333 VTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAA 392
           VTL+ASLAAR+GSTSMAAFQVCLQVWLA SLLADG AVAGQ ILASAFA+ D+ +A   A
Sbjct: 320 VTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAATA 379

Query: 393 SRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFD 452
           SRVLQLGL+LG  L V +GAG+ FGA++FT D  VLHLI IG PF+A  QPINALAFVFD
Sbjct: 380 SRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVFD 439

Query: 453 GINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGT 476
           G+NFGASDF Y+A S+V+VAI+SI CL  LSST GFIG+W  LTIYMSLR   GFWR+GT
Sbjct: 440 GVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIGT 499

BLAST of Cp4.1LG05g05060 vs. TAIR 10
Match: AT3G08040.1 (MATE efflux family protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.3e-138
Identity = 284/507 (56.02%), Postives = 346/507 (68.24%), Query Frame = 0

Query: 18  KTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLF-------------- 77
           K  +P  + FKD RHVF  D  GREI  IA PAALALAADP+ASL               
Sbjct: 12  KKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLA 71

Query: 78  ------------QELQFSPFV--TTSFVAEEDAIGSACNEAKDNN-----------DKET 137
                         +   P V  TTSFVAEED +     EA   N             E 
Sbjct: 72  AVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEK 131

Query: 138 GL---FTNDESKLMIPHNGKTEENG--------RRYIPSASSALVIGGVLGLIQAIFLIS 197
           G+    +ND ++   P    T+ N         +R I +AS+A+++G +LGL+QAIFLI 
Sbjct: 132 GISSPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIF 191

Query: 198 GAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVA 257
            +K LL  MGVK +SPM++ A +YL++R+LGAPA+LLSLAMQG+FRGFKDTKTPLFATV 
Sbjct: 192 SSKLLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVV 251

Query: 258 GDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSR 317
            D  NI+LDPIFIF+ RLG+ GAAIAHVISQY + LILF  L  +V+L+PP+   LQF R
Sbjct: 252 ADVINIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGR 311

Query: 318 FLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQT 377
           FLKNG LLL R IAVTFC TLAA++AAR G+T MAAFQ+CLQVWL  SLL DGLAVAGQ 
Sbjct: 312 FLKNGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQA 371

Query: 378 ILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIG 437
           ILA +FA+ D++K T  ASRVLQ+G +LGLGL VFVG G+ FGA +F+ D  V+HL+ IG
Sbjct: 372 ILACSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIG 431

Query: 438 TPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVA 475
            PFIAA QPIN+LAFV DG+NFGASDFAY+AYSMV VA ISI  +  ++ T GFIGIW+A
Sbjct: 432 IPFIAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIA 491

BLAST of Cp4.1LG05g05060 vs. TAIR 10
Match: AT3G08040.2 (MATE efflux family protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.3e-138
Identity = 284/507 (56.02%), Postives = 346/507 (68.24%), Query Frame = 0

Query: 18  KTRMPIRIFFKDARHVFKLDELGREIAQIALPAALALAADPVASLF-------------- 77
           K  +P  + FKD RHVF  D  GREI  IA PAALALAADP+ASL               
Sbjct: 12  KKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLA 71

Query: 78  ------------QELQFSPFV--TTSFVAEEDAIGSACNEAKDNN-----------DKET 137
                         +   P V  TTSFVAEED +     EA   N             E 
Sbjct: 72  AVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEK 131

Query: 138 GL---FTNDESKLMIPHNGKTEENG--------RRYIPSASSALVIGGVLGLIQAIFLIS 197
           G+    +ND ++   P    T+ N         +R I +AS+A+++G +LGL+QAIFLI 
Sbjct: 132 GISSPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIF 191

Query: 198 GAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLAMQGVFRGFKDTKTPLFATVA 257
            +K LL  MGVK +SPM++ A +YL++R+LGAPA+LLSLAMQG+FRGFKDTKTPLFATV 
Sbjct: 192 SSKLLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVV 251

Query: 258 GDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFWRLMGQVDLLPPSIKHLQFSR 317
            D  NI+LDPIFIF+ RLG+ GAAIAHVISQY + LILF  L  +V+L+PP+   LQF R
Sbjct: 252 ADVINIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGR 311

Query: 318 FLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVCLQVWLAMSLLADGLAVAGQT 377
           FLKNG LLL R IAVTFC TLAA++AAR G+T MAAFQ+CLQVWL  SLL DGLAVAGQ 
Sbjct: 312 FLKNGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQA 371

Query: 378 ILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGMTFGAKLFTSDVDVLHLIGIG 437
           ILA +FA+ D++K T  ASRVLQ+G +LGLGL VFVG G+ FGA +F+ D  V+HL+ IG
Sbjct: 372 ILACSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIG 431

Query: 438 TPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAIISIFCLFILSSTQGFIGIWVA 475
            PFIAA QPIN+LAFV DG+NFGASDFAY+AYSMV VA ISI  +  ++ T GFIGIW+A
Sbjct: 432 IPFIAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIA 491

BLAST of Cp4.1LG05g05060 vs. TAIR 10
Match: AT2G38330.1 (MATE efflux family protein )

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-91
Identity = 205/468 (43.80%), Postives = 282/468 (60.26%), Query Frame = 0

Query: 36  LDELGREIAQIALPAALALAADPVASLFQE---------------LQFSPF--------- 95
           + ++G EI  IALPAALALAADP+ SL                  +  S F         
Sbjct: 73  IGKIGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNV 132

Query: 96  ----VTTSFVAEEDAIGSACNEAKDNNDKETGLFTNDESKLMIPHNGKTEENGRRYIPSA 155
               VTTSFVAEE AI      AKD+ND                    + E  ++ +PS 
Sbjct: 133 PLLNVTTSFVAEEQAIA-----AKDDND--------------------SIETSKKVLPSV 192

Query: 156 SSALVIGGVLGLIQAIFLISGAKPLLNFMGVKSDSPMMTSAQQYLTLRSLGAPAVLLSLA 215
           S++LV+   +G+ +AI L  G+  L++ M +  DSPM   A+Q+L LR+ GAP ++++LA
Sbjct: 193 STSLVLAAGVGIAEAIALSLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALA 252

Query: 216 MQGVFRGFKDTKTPLFATVAGDATNIILDPIFIFIFRLGVSGAAIAHVISQYLIALILFW 275
            QG FRGFKDT TPL+A VAG+  N +LDPI IF+   G+SGAA A VIS+YLIA IL W
Sbjct: 253 AQGAFRGFKDTTTPLYAVVAGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLW 312

Query: 276 RLMGQVDLLPPSIKHLQFSRFLKNGFLLLMRVIAVTFCVTLAASLAARQGSTSMAAFQVC 335
           +L   V LL P IK  + +++LK+G LL+ R +A+    TLA SLAA+ G T MA  Q+ 
Sbjct: 313 KLNENVVLLSPQIKVGRANQYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIV 372

Query: 336 LQVWLAMSLLADGLAVAGQTILASAFAQNDHDKATTAASRVLQLGLLLGLGLGVFVGAGM 395
           L++WLA+SLL D LA+A Q++LA+ ++Q ++ +A      VLQ+GL  G GL   +    
Sbjct: 373 LEIWLAVSLLTDALAIAAQSLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITF 432

Query: 396 TFGAKLFTSDVDVLHLIGIGTPFIAAMQPINALAFVFDGINFGASDFAYSAYSMVLVAII 455
              + LFT+D +VL +   GT F+A  QP+NALAFV DG+ +G SDF ++AYSMV+V  I
Sbjct: 433 EPFSSLFTTDSEVLKIALSGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFI 492

Query: 456 SIFCLFILSSTQGFIGIWVALTIYMSLRTLAGFWRVGTGTGPWYFLQS 476
           S   + + + T G  GIW  L ++M+LR +AG WR+GT TGPW  L S
Sbjct: 493 SSLFMLVAAPTFGLAGIWTGLFLFMALRLVAGAWRLGTRTGPWKMLWS 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYD61.2e-16064.21Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2[more]
Q9SFB01.8e-13756.02Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1[more]
Q84K711.7e-9043.80Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX4... [more]
Q9SVE73.9e-7937.75Protein DETOXIFICATION 45, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX4... [more]
Q2FJS91.1e-0925.00Multidrug export protein MepA OS=Staphylococcus aureus (strain USA300) OX=367830... [more]
Match NameE-valueIdentityDescription
XP_023532550.13.05e-31593.04protein DETOXIFICATION 42-like [Cucurbita pepo subsp. pepo][more]
KAG7035862.15.96e-31492.64Protein DETOXIFICATION 42 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022957625.11.70e-31392.45protein DETOXIFICATION 42-like [Cucurbita moschata][more]
XP_022995068.13.41e-31392.45protein DETOXIFICATION 42-like [Cucurbita maxima][more]
KAG6605914.17.85e-29892.55Protein DETOXIFICATION 42, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1H1288.21e-31492.45Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459102 PE=3 SV=1[more]
A0A6J1K6V01.65e-31392.45Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111490726 PE=3 SV=1[more]
A0A1S3BM074.60e-27681.19Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103491512 PE=3 SV=1[more]
A0A5D3BAV34.60e-27681.19Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A6J1D6604.22e-27480.69Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111017410 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G51340.28.4e-16264.21MATE efflux family protein [more]
AT1G51340.11.2e-15764.49MATE efflux family protein [more]
AT3G08040.11.3e-13856.02MATE efflux family protein [more]
AT3G08040.21.3e-13856.02MATE efflux family protein [more]
AT2G38330.11.2e-9143.80MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 127..218
e-value: 1.5E-10
score: 40.9
coord: 277..430
e-value: 2.4E-12
score: 46.8
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 72..445
e-value: 2.3E-108
score: 360.6
NoneNo IPR availablePANTHERPTHR42893:SF34PROTEIN DETOXIFICATIONcoord: 71..474
coord: 21..64
IPR044644Multi antimicrobial extrusion protein DinF-likePANTHERPTHR42893PROTEIN DETOXIFICATION 44, CHLOROPLASTIC-RELATEDcoord: 71..474
coord: 21..64
IPR044644Multi antimicrobial extrusion protein DinF-likeCDDcd13136MATE_DinF_likecoord: 124..465
e-value: 4.00589E-97
score: 297.165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g05060.1Cp4.1LG05g05060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0042908 xenobiotic transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity