Cp4.1LG05g02560 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g02560
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	DNA mismatch repair protein MSH4
Location	Cp4.1LG05: 1456818 .. 1458906 (+)
RNA-Seq Expression	Cp4.1LG05g02560
Synteny	Cp4.1LG05g02560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGGAGTTGCTGCGTTCGATTTGAGATCAGCTTCACTTCATCTTTCTCAATATATAGAGACCAGCAGCTCCTACCAGAATACAAAAACTTTGCTGCATTTCTATGATCCAATGGTGATTCTAGTTCCTCCCAACAAGCTCGCACCTGATGGCATGGTTGGAGTTTCTGTTTTGGTAGACAGATTTTATGTTACAGTGAAGAAGGTAAGTTCATATTCACCGAGTTCGTAACTCCTTCACTCAGCATGCAGTGTCAGTGAAGATTGCTTACTTTTACAGAGAAACTTAAATTGAATGCAAAACTCTGTAGGTTGTAATGGCTCGTGGTTGCTTTGACGACACAAAGGCATTTTCCTTTTTCTTTCCTCTCTTAAAGTTTTATTTTATGCATCTCAGTTTCATATGGTTATTGTATTGGATGCTGTTCTTTAATCAGTATGCTCAGATCATATCTTAGTCGAACTTTCCAGGGTGCTGTTTTGATTAAGAATCTGGCAGCCAAGGAGCCTTCTGCTCTTGGTTTGGAAACTTATTACAAACAGTACTATCTCTGCTTGGCTGCTGCTGCTGCTAGCATTAAATGGTATTTTCAGAACTTCAGCCTTCTATAAGTCACATATGGGAAGTGTTATCTATAGTTAACTAATAAGAAGAGAGAATTTTTTTAATGCTTAGTTGTGACTAATGATCCAGCTTCTTGATGCACAGGATAGAAGCAGAGAAGGGGGTTATTGTGACCAATCACTCTTTAACGGTAAATTTGCCTTTCTGAAATGGTAATGTATAGAATTCAATGGTTTGATTTTCTCCTGATGCTTGAATGAGATTTCGCTGTAAGACAAACGATCTCGTAGTGACCAATATAATTGAATTGAAAAGTCAAGTTTCTGTAGTTCGGTTAAGTTCTCTTCCCAGTTTTCTCGTGTGTAAGATTGCTGACCTAATGCTGGGAAATTATTTAATTTAATTTTTCTTTCCTTTCCTCTTGATTGAGTGCGTAGAACGGATGCACCTTATTTCCTCTTATTCTAACCTCTAGACAAGGCTTTTACTATGCATTGGTTGAGTTGCGAATCATTTTGACTCTTTGTTTATAAGTTTTTTTAATTCGGTATGGGATTTCAATTTTCTCAGCAGCTCATTCCTCTTTCACCTTTTGTTGTTCTACTTTCTAGATTTGTGTTTTGAAAGATAAAGTACTCTGAACAGGACTAAAGATTCTGTCCTGTGGAGGTGTAATTAACTAACTTTAGGAACTAGGCTTAAAACAATGTTGCCTCTCTGATTCGAACATCATTTTTATTAGTCTTCGGAGAGAATATGAACGAGACGTTCATTTATTTCTGGAGTTCTTCCTTCATCCTGATGGTTCTCAGTTGTAACTTTTAGGTCACATTTAATGGTTCATCTGATCATGTGAGCATTGATGCAACGAGGTTCCCTCCATTTCTCTTTCCTTTTCAAATATGTGTTACTGCTGATCGCAATATCAATGTCCAAGTCATTGATTTATATTTTTCATACTTTTCGGAGAACATTTTCTTGAGAATTGGCCACAAAATGTTGGTTTCAAATTACTGATTAATGCTGAATTATATGCTGACAGTGTTCACAATTTAGAAATTATTGAGCCACTTCACTCCAACCTTTGGGGAACAAGCAACAAGAAGAGAAGTCTGTTCCACATGCTCAAGACAACAAAAACTATAGGAGGGTACGAGGATACCTTAGGAATGTTGATATATTTTTCTTCATTTGTCTACCAATTGCTGCAAAAATTTATTTTGTTCCCTCTTCGTGTTATCATCTAAACTAGTATTTGAAGTCCTCTCGTTTATTACATCTTATCTTGGGGCGGGAAGTTTTGATGACATGTAATTGACCAAAGGGAAAAAATTATTTTGATATGCAGTTATTGCCAAATGCTCGTCTAAAATGTTTTTAGTCCCGCTCGTATCTCAAAATTTTATGCTTTACTCTCTCACTGCAATTTTGGTCAAGTTTTTGTTACAGGTCTAGACTTCTTCGTGCCAATCTTTTGCAGCCATTAAAAGATATTGAAACCATTAATGCCCGTCTGGATTGCCTG

mRNA sequence

GTTGGAGTTGCTGCGTTCGATTTGAGATCAGCTTCACTTCATCTTTCTCAATATATAGAGACCAGCAGCTCCTACCAGAATACAAAAACTTTGCTGCATTTCTATGATCCAATGGTGATTCTAGTTCCTCCCAACAAGCTCGCACCTGATGGCATGGTTGGAGTTTCTGTTTTGGTAGACAGATTTTATGTTACAGTGAAGAAGGGTGCTGTTTTGATTAAGAATCTGGCAGCCAAGGAGCCTTCTGCTCTTGGTTTGGAAACTTATTACAAACAGTACTATCTCTGCTTGGCTGCTGCTGCTGCTAGCATTAAATGGATAGAAGCAGAGAAGGGGGTTATTGTGACCAATCACTCTTTAACGTTCTTCCTTCATCCTGATGGTTCTCAGTTTGTTCACAATTTAGAAATTATTGAGCCACTTCACTCCAACCTTTGGGGAACAAGCAACAAGAAGAGAAGTCTGTTCCACATGCTCAAGACAACAAAAACTATAGGAGGGTCTAGACTTCTTCGTGCCAATCTTTTGCAGCCATTAAAAGATATTGAAACCATTAATGCCCGTCTGGATTGCCTG

Coding sequence (CDS)

GTTGGAGTTGCTGCGTTCGATTTGAGATCAGCTTCACTTCATCTTTCTCAATATATAGAGACCAGCAGCTCCTACCAGAATACAAAAACTTTGCTGCATTTCTATGATCCAATGGTGATTCTAGTTCCTCCCAACAAGCTCGCACCTGATGGCATGGTTGGAGTTTCTGTTTTGGTAGACAGATTTTATGTTACAGTGAAGAAGGGTGCTGTTTTGATTAAGAATCTGGCAGCCAAGGAGCCTTCTGCTCTTGGTTTGGAAACTTATTACAAACAGTACTATCTCTGCTTGGCTGCTGCTGCTGCTAGCATTAAATGGATAGAAGCAGAGAAGGGGGTTATTGTGACCAATCACTCTTTAACGTTCTTCCTTCATCCTGATGGTTCTCAGTTTGTTCACAATTTAGAAATTATTGAGCCACTTCACTCCAACCTTTGGGGAACAAGCAACAAGAAGAGAAGTCTGTTCCACATGCTCAAGACAACAAAAACTATAGGAGGGTCTAGACTTCTTCGTGCCAATCTTTTGCAGCCATTAAAAGATATTGAAACCATTAATGCCCGTCTGGATTGCCTG

Protein sequence

VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVDRFYVTVKKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIEAEKGVIVTNHSLTFFLHPDGSQFVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTTKTIGGSRLLRANLLQPLKDIETINARLDCL

Homology

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy Swiss-Prot
Match: F4JP48 (DNA mismatch repair protein MSH4 OS=Arabidopsis thaliana OX=3702 GN=MSH4 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.0e-75
Identity = 156/210 (74.29%), Postives = 170/210 (80.95%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VG+AAFDLRSASLHLSQYIETSSSYQNTKTLL FYDP VI+VPPNKLA DGMVGVS LVD
Sbjct: 23  VGMAAFDLRSASLHLSQYIETSSSYQNTKTLLRFYDPSVIIVPPNKLAADGMVGVSELVD 82

Query: 61  RFYVTVK------------KGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           R Y TV+            KGAVLI+NLAA+EP ALGL+TYYKQ+YL LAAAAA+IKWIE
Sbjct: 83  RCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGLDTYYKQHYLSLAAAAATIKWIE 142

Query: 121 AEKGVIVTNHSLTF-----FLHPD-GSQFVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT      F H +  +  V NLE+I+P H+ L GTSNKKRSLF M KTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 193
           KT GG+RLLRANLLQPLKDIETIN RLDCL
Sbjct: 203 KTAGGTRLLRANLLQPLKDIETINTRLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy Swiss-Prot
Match: Q99MT2 (MutS protein homolog 4 OS=Mus musculus OX=10090 GN=Msh4 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.6e-15
Identity = 68/210 (32.38%), Postives = 103/210 (49.05%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVL-- 60
           +G+A+ DL+S  + LSQ+ + +++Y    T L    P+ I++            +  L  
Sbjct: 193 IGMASIDLKSPQIMLSQFAD-NTTYAKVITKLQVLSPLEIIMSNTACVVGNSTKLFTLIT 252

Query: 61  ----------VDRFYVTVKKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
                     V R Y    KG   I+ L   E S++ +E   +  Y CLAAAAA +K++E
Sbjct: 253 ENFKNVNFTTVQRKYFNETKGLEYIEQLCIAEFSSVLMEV--QSRYYCLAAAAALLKYVE 312

Query: 121 AEKGVIVTNHSLTFFLHPD------GSQFVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
             +  +    SL  +           S    NLE+   L +N    SN   +LF +L  T
Sbjct: 313 FIQNSVYAPKSLKIYFQGSEQTAMIDSSSAQNLEL---LVNNQDYRSN--HTLFGVLNYT 372

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 193
           KT GGSR LR+N+L+PL D+ETI+ RLDC+
Sbjct: 373 KTAGGSRRLRSNILEPLVDVETISMRLDCV 394

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy Swiss-Prot
Match: O15457 (MutS protein homolog 4 OS=Homo sapiens OX=9606 GN=MSH4 PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.0e-14
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVL-- 60
           +G+A+ DL++  + LSQ+ + +++Y    T L    P+ I++     A      +  L  
Sbjct: 171 IGMASIDLKNPQIILSQFAD-NTTYAKVITKLKILSPLEIIMSNTACAVGNSTKLFTLIT 230

Query: 61  ----------VDRFYVTVKKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
                     + R Y    KG   I+ L   E S + +E   K  Y CLAA AA +K++E
Sbjct: 231 ENFKNVNFTTIQRKYFNETKGLEYIEQLCIAEFSTVLMEVQSK--YYCLAAVAALLKYVE 290

Query: 121 AEKGVIVTNHSLTFFLHPD------GSQFVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
             +  +    SL              S    NLE+   L +N    +N   +LF +L  T
Sbjct: 291 FIQNSVYAPKSLKICFQGSEQTAMIDSSSAQNLEL---LINNQDYRNN--HTLFGVLNYT 350

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 193
           KT GGSR LR+N+L+PL DIETIN RLDC+
Sbjct: 351 KTPGGSRRLRSNILEPLVDIETINMRLDCV 372

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy Swiss-Prot
Match: Q8UIF2 (DNA mismatch repair protein MutS OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=mutS PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 9.7e-05
Identity = 37/104 (35.58%), Postives = 56/104 (53.85%), Query Frame = 0


Query: 97  LAAAAASIKWIE----AEKGVIVT----NHSLTFFLHPDGSQFVHNLEIIEPLHSNLWGT 156
           +AAAAA++ ++E    AE+  +      + + T F+ P       NLE+++ L      +
Sbjct: 255 MAAAAAAVAYVEKTQIAERPPLGAPERESAASTLFIDPATRA---NLELVKTL------S 314

Query: 157 SNKKRSLFHMLKTTKTIGGSRLLRANLLQPLKDIETINARLDCL 193
            ++  SL H L  T T GG+RLL   L+ PL D E INARLD +
Sbjct: 315 GDRDGSLLHALNRTVTGGGARLLAERLMSPLTDPERINARLDAV 349

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy Swiss-Prot
Match: Q1IN52 (DNA mismatch repair protein MutS OS=Koribacter versatilis (strain Ellin345) OX=204669 GN=mutS PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.2e-04
Identity = 29/61 (47.54%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 132 VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTTKTIGGSRLLRANLLQPLKDIETINARLDC 191
           V NLE+IEPL +N    + +  +LF  L  T T  G RLLRA +L+P  D   INARLD 
Sbjct: 288 VRNLELIEPLFTN----TGEGVTLFRALDATMTPMGKRLLRAWMLRPSIDTSEINARLDA 344

Query: 192 L 193
           +
Sbjct: 348 I 344

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BLAST of Cp4.1LG05g02560 vs. NCBI nr
Match: XP_023521478.1 (DNA mismatch repair protein MSH4-like [Cucurbita pepo subsp. pepo] >XP_023532701.1 DNA mismatch repair protein MSH4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 342 bits (878), Expect = 3.40e-110
Identity = 182/210 (86.67%), Postives = 183/210 (87.14%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           RFYVTVKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  RFYVTVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT   +            VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSSDHVSIDATSVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. NCBI nr
Match: XP_022957703.1 (DNA mismatch repair protein MSH4 [Cucurbita moschata])

HSP 1 Score: 338 bits (867), Expect = 1.47e-108
Identity = 180/210 (85.71%), Postives = 182/210 (86.67%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           +FYVTVKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  KFYVTVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT   +            V NLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. NCBI nr
Match: XP_022996171.1 (DNA mismatch repair protein MSH4 [Cucurbita maxima])

HSP 1 Score: 338 bits (866), Expect = 2.07e-108
Identity = 180/210 (85.71%), Postives = 182/210 (86.67%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           RFYV+VKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  RFYVSVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT   +            V NLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. NCBI nr
Match: XP_022154824.1 (DNA mismatch repair protein MSH4 [Momordica charantia])

HSP 1 Score: 335 bits (858), Expect = 3.28e-107
Identity = 178/210 (84.76%), Postives = 180/210 (85.71%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVL D
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLAD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           RFY TVKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  RFYATVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSL+   +            V NLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLSVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. NCBI nr
Match: KAG7036113.1 (DNA mismatch repair protein MSH4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333 bits (855), Expect = 5.80e-107
Identity = 181/225 (80.44%), Postives = 182/225 (80.89%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 82

Query: 61  RFYVTVKK---------------------------GAVLIKNLAAKEPSALGLETYYKQY 120
           RFYVTVKK                           GAVLIKNLAAKEPSALGLETYYKQY
Sbjct: 83  RFYVTVKKFYFMHLSFIWLLYWMLMLRSYLSRTFQGAVLIKNLAAKEPSALGLETYYKQY 142

Query: 121 YLCLAAAAASIKWIEAEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWG 180
           YLCLAAAAASIKWIEAEKGVIVTNHSLT   +            V NLEIIEPLHSNLWG
Sbjct: 143 YLCLAAAAASIKWIEAEKGVIVTNHSLTVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWG 202

Query: 181 TSNKKRSLFHMLKTTKTIGGSRLLRANLLQPLKDIETINARLDCL 192
           TSNKKRSLFHMLKTTKTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 TSNKKRSLFHMLKTTKTIGGSRLLRANLLQPLKDIETINARLDCL 247

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy TrEMBL
Match: A0A6J1H107 (DNA mismatch repair protein MSH4 OS=Cucurbita moschata OX=3662 GN=LOC111459167 PE=4 SV=1)

HSP 1 Score: 338 bits (867), Expect = 7.13e-109
Identity = 180/210 (85.71%), Postives = 182/210 (86.67%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           +FYVTVKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  KFYVTVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT   +            V NLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy TrEMBL
Match: A0A6J1K3Y6 (DNA mismatch repair protein MSH4 OS=Cucurbita maxima OX=3661 GN=LOC111491480 PE=4 SV=1)

HSP 1 Score: 338 bits (866), Expect = 1.00e-108
Identity = 180/210 (85.71%), Postives = 182/210 (86.67%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           RFYV+VKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  RFYVSVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT   +            V NLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy TrEMBL
Match: A0A6J1DNB3 (DNA mismatch repair protein MSH4 OS=Momordica charantia OX=3673 GN=LOC111021986 PE=4 SV=1)

HSP 1 Score: 335 bits (858), Expect = 1.59e-107
Identity = 178/210 (84.76%), Postives = 180/210 (85.71%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVL D
Sbjct: 23  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLAD 82

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           RFY TVKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 83  RFYATVKKVVMARGCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 142

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSL+   +            V NLEIIEPLHSNLWGTSNKKRSLFHMLKTT
Sbjct: 143 AEKGVIVTNHSLSVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 203 KTIGGSRLLRANLLQPLKDIETINARLDCL 232

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy TrEMBL
Match: A0A7J6XDD3 (DNA mismatch repair protein MutS OS=Thalictrum thalictroides OX=46969 GN=FRX31_002930 PE=4 SV=1)

HSP 1 Score: 308 bits (789), Expect = 3.80e-104
Identity = 164/210 (78.10%), Postives = 179/210 (85.24%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFD+RSASLHLSQYIETSSSYQNTKTLLHFYDPMVI+VPPNKLA DGMVGVS LVD
Sbjct: 24  VGVAAFDMRSASLHLSQYIETSSSYQNTKTLLHFYDPMVIIVPPNKLAADGMVGVSELVD 83

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           R Y   KK            G+VL+K+LAAKEPSALGL+TYYKQYYLCLAA +A+IKWIE
Sbjct: 84  RSYAASKKVVMARGCFDDTKGSVLVKSLAAKEPSALGLDTYYKQYYLCLAAVSATIKWIE 143

Query: 121 AEKGVIVTNHSL--TF---FLHPD-GSQFVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSL  TF   F H +  +  V NLEI+EPLHSNLWG+SNKKRSLFHMLKTT
Sbjct: 144 AEKGVIVTNHSLLVTFNGSFDHMNIDATSVQNLEIVEPLHSNLWGSSNKKRSLFHMLKTT 203

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           +TIGG+RLLRANLLQPLKDIET+NARLDCL
Sbjct: 204 RTIGGTRLLRANLLQPLKDIETLNARLDCL 233

[Close]

BLAST of Cp4.1LG05g02560 vs. ExPASy TrEMBL
Match: A0A5A7TER1 (DNA mismatch repair protein MSH4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00610 PE=4 SV=1)

HSP 1 Score: 325 bits (834), Expect = 5.33e-104
Identity = 174/210 (82.86%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILV PNKLAPDGMVGVSVL D
Sbjct: 20  VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVSPNKLAPDGMVGVSVLAD 79

Query: 61  RFYVTVKK------------GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           RF+ TVKK            GAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE
Sbjct: 80  RFFATVKKVVMARSCFDDTKGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 139

Query: 121 AEKGVIVTNHSLTFFLHPDGSQF------VHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSL    +            V NLEIIEPLHSNLWGTSNKKRSL++MLKTT
Sbjct: 140 AEKGVIVTNHSLLVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTT 199

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 192
           KTIGGSRLLRANLLQPLKDIETINARLDCL
Sbjct: 200 KTIGGSRLLRANLLQPLKDIETINARLDCL 229

[Close]

BLAST of Cp4.1LG05g02560 vs. TAIR 10
Match: AT4G17380.1 (MUTS-like protein 4 )

HSP 1 Score: 282.3 bits (721), Expect = 2.8e-76
Identity = 156/210 (74.29%), Postives = 170/210 (80.95%), Query Frame = 0


Query: 1   VGVAAFDLRSASLHLSQYIETSSSYQNTKTLLHFYDPMVILVPPNKLAPDGMVGVSVLVD 60
           VG+AAFDLRSASLHLSQYIETSSSYQNTKTLL FYDP VI+VPPNKLA DGMVGVS LVD
Sbjct: 23  VGMAAFDLRSASLHLSQYIETSSSYQNTKTLLRFYDPSVIIVPPNKLAADGMVGVSELVD 82

Query: 61  RFYVTVK------------KGAVLIKNLAAKEPSALGLETYYKQYYLCLAAAAASIKWIE 120
           R Y TV+            KGAVLI+NLAA+EP ALGL+TYYKQ+YL LAAAAA+IKWIE
Sbjct: 83  RCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGLDTYYKQHYLSLAAAAATIKWIE 142

Query: 121 AEKGVIVTNHSLTF-----FLHPD-GSQFVHNLEIIEPLHSNLWGTSNKKRSLFHMLKTT 180
           AEKGVIVTNHSLT      F H +  +  V NLE+I+P H+ L GTSNKKRSLF M KTT
Sbjct: 143 AEKGVIVTNHSLTVTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTT 202

Query: 181 KTIGGSRLLRANLLQPLKDIETINARLDCL 193
           KT GG+RLLRANLLQPLKDIETIN RLDCL
Sbjct: 203 KTAGGTRLLRANLLQPLKDIETINTRLDCL 232

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g02560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JP48	4.0e-75	74.29	DNA mismatch repair protein MSH4 OS=Arabidopsis thaliana OX=3702 GN=MSH4 PE=2 SV...	[more]
Q99MT2	3.6e-15	32.38	MutS protein homolog 4 OS=Mus musculus OX=10090 GN=Msh4 PE=2 SV=1	[more]
O15457	3.0e-14	32.38	MutS protein homolog 4 OS=Homo sapiens OX=9606 GN=MSH4 PE=1 SV=2	[more]
Q8UIF2	9.7e-05	35.58	DNA mismatch repair protein MutS OS=Agrobacterium fabrum (strain C58 / ATCC 3397...	[more]
Q1IN52	2.2e-04	47.54	DNA mismatch repair protein MutS OS=Koribacter versatilis (strain Ellin345) OX=2...	[more]

BLAST of Cp4.1LG05g02560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023521478.1	3.40e-110	86.67	DNA mismatch repair protein MSH4-like [Cucurbita pepo subsp. pepo] >XP_023532701...	[more]
XP_022957703.1	1.47e-108	85.71	DNA mismatch repair protein MSH4 [Cucurbita moschata]	[more]
XP_022996171.1	2.07e-108	85.71	DNA mismatch repair protein MSH4 [Cucurbita maxima]	[more]
XP_022154824.1	3.28e-107	84.76	DNA mismatch repair protein MSH4 [Momordica charantia]	[more]
KAG7036113.1	5.80e-107	80.44	DNA mismatch repair protein MSH4, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of Cp4.1LG05g02560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H107	7.13e-109	85.71	DNA mismatch repair protein MSH4 OS=Cucurbita moschata OX=3662 GN=LOC111459167 P...	[more]
A0A6J1K3Y6	1.00e-108	85.71	DNA mismatch repair protein MSH4 OS=Cucurbita maxima OX=3661 GN=LOC111491480 PE=...	[more]
A0A6J1DNB3	1.59e-107	84.76	DNA mismatch repair protein MSH4 OS=Momordica charantia OX=3673 GN=LOC111021986 ...	[more]
A0A7J6XDD3	3.80e-104	78.10	DNA mismatch repair protein MutS OS=Thalictrum thalictroides OX=46969 GN=FRX31_0...	[more]
A0A5A7TER1	5.33e-104	82.86	DNA mismatch repair protein MSH4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of Cp4.1LG05g02560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G17380.1	2.8e-76	74.29	MUTS-like protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007696	DNA mismatch repair protein MutS, core	PFAM	PF05192	MutS_III	coord: 133..191 e-value: 3.0E-15 score: 57.0
None	No IPR available	GENE3D	1.10.1420.10		coord: 129..192 e-value: 7.1E-17 score: 64.3
None	No IPR available	PANTHER	PTHR11361:SF21	MUTS PROTEIN HOMOLOG 4	coord: 24..192
IPR045076	DNA mismatch repair MutS family	PANTHER	PTHR11361	DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER	coord: 24..192
IPR036187	DNA mismatch repair protein MutS, core domain superfamily	SUPERFAMILY	48334	DNA repair protein MutS, domain III	coord: 133..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g02560.1	Cp4.1LG05g02560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007129	homologous chromosome pairing at meiosis
biological_process	GO:0010777	meiotic mismatch repair involved in reciprocal meiotic recombination
biological_process	GO:0006857	oligopeptide transport
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0006298	mismatch repair
cellular_component	GO:0000794	condensed nuclear chromosome
cellular_component	GO:0043073	germ cell nucleus
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009705	plant-type vacuole membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0030983	mismatched DNA binding
molecular_function	GO:0022857	transmembrane transporter activity