Homology
BLAST of Cp4.1LG05g02130 vs. ExPASy Swiss-Prot
Match:
B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)
HSP 1 Score: 498.0 bits (1281), Expect = 1.2e-139
Identity = 245/489 (50.10%), Postives = 345/489 (70.55%), Query Frame = 0
Query: 7 LCLIGA-LAGVYVFLFGILRNLNQWRF-VTRHARRYNLPPGDMGWPLIGTLLPFLKAFRS 66
+C+ A L Y+F +RNLN W + V + + LPPGDMGWPLIG LL F+K F S
Sbjct: 6 VCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSS 65
Query: 67 GRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILT 126
G PDSFI++ KYG++G+YKTHLFG+PS+IV P++CR VL +D F GY K+ + L
Sbjct: 66 GHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELA 125
Query: 127 GSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSE 186
+ + V EHR RRLI S I G++AL++Y+ +E IV+ LEE SMK PVE L E
Sbjct: 126 RCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKE 185
Query: 187 MKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIR 246
MK V+FK ++H+F+G+++ I K+ + D + G S +++PG TF +AL+AR +L +
Sbjct: 186 MKKVSFKAIVHVFMGSSNQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAK 245
Query: 247 IFEKVLEEKRENLKNKVGE-NRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHET 306
I + V++E+R ++N E +++KD+ D+LLEV+DE+G L+D I DLLIG FAGHE+
Sbjct: 246 IVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHES 305
Query: 307 SAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDET 366
+A ++M +I +L+++P L+KAK EQE+I R + K L++KEIKQM YLS+V+DET
Sbjct: 306 TATSLMWSITYLTQHPHILKKAKEEQEEITRTR--FSSQKQLSLKEIKQMVYLSQVIDET 365
Query: 367 LRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPK 426
LR ++AF RE DVN+NGY IPKGW++L+W RA+HM+ E Y NP +F+PSRWD+
Sbjct: 366 LRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYN 425
Query: 427 RRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDN 486
+AG+F+PFGAG RLCPG DL+KLEI+IFLHYF+ +RLER+NP C LP+ PTDN
Sbjct: 426 AKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLR-NYRLERINPECHVTSLPVSKPTDN 485
Query: 487 CLARVVRDS 493
CLA+V++ S
Sbjct: 486 CLAKVIKVS 491
BLAST of Cp4.1LG05g02130 vs. ExPASy Swiss-Prot
Match:
O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 460.3 bits (1183), Expect = 2.7e-128
Identity = 227/470 (48.30%), Postives = 325/470 (69.15%), Query Frame = 0
Query: 23 ILRNLNQWRFVTR-HARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKT 82
++R +N W + + R+ LPPGD+GWP IG +L FL+AF++ PDSF +YG
Sbjct: 25 LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84
Query: 83 GMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNSVPKPEHRRLR 142
G+YK H+FG+PS+IVT + CR VL +D+ F G+ +T L G K+ + EH+RLR
Sbjct: 85 GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144
Query: 143 RLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKVLLHIFIGAN 202
RL A+ ++G+EALS YI ++E V+T L++W M E EFL+ ++ + F+++++IF+ +
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204
Query: 203 SAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKV 262
S +D +E+ Y + G + AV+IPG + RALKAR L+ F+ ++ E+R K +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264
Query: 263 GENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPET 322
N KKDM D LL V+DEDG+ LDD IID+L+ + AGHE+S HTIM A +FL E+PE
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324
Query: 323 LRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDV 382
L++AKAEQE I+ +RP KGL++KE ++ME+LS+V+DETLR + + T RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384
Query: 383 NLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 442
+NGY IPKGWK+L W R VH++PE++ +P KFDP+RWDN PK AG+F+PFGAG L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444
Query: 443 CPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARV 489
CPG DL+KLEI+IFLH+F+ ++++R NP CP +LP PTDNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of Cp4.1LG05g02130 vs. ExPASy Swiss-Prot
Match:
Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)
HSP 1 Score: 454.5 bits (1168), Expect = 1.5e-126
Identity = 224/479 (46.76%), Postives = 327/479 (68.27%), Query Frame = 0
Query: 13 LAGVYVFLFGILRNLNQWRFVTR-HARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFI 72
+ G++V L +L+ +N W +V++ +++ LPPGD+GWP+IG + FL+AF++ P+SFI
Sbjct: 15 ILGLFV-LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFI 74
Query: 73 HHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNS 132
+ ++YG+TG+YK H+FG P V+VT PE CR VL +D+ F G+ K+T L G K+
Sbjct: 75 QSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVG 134
Query: 133 VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFK 192
+ EH+RLRRL ++ ++G EALS+YI +E V T LE+W M E +EFLS ++ + FK
Sbjct: 135 ISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFK 194
Query: 193 VLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLE 252
V+++IF+ + S +D +E+ Y + + G + +++PG + RALKAR +L+ F+ ++
Sbjct: 195 VIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVT 254
Query: 253 EKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRA 312
+R K + NR KDM D L++V+DE+G LDD IIDLL+ + AGHE+S H M A
Sbjct: 255 NRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWA 314
Query: 313 IMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAF 372
+ + E+P L+KAK EQE+IV R A + LT+KE ++M YLS+V+DETLR + +
Sbjct: 315 TILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDETLRVITFSL 374
Query: 373 TLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIP 432
T RE K DV ++GY IPKGWK+L W R VH++PEIY +P KFDPSRW+ +AG+F+P
Sbjct: 375 TAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLP 434
Query: 433 FGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARVVR 491
FG G LCPG DL+KLEI+IFLH+F+ +R+ER NP CP LP P DNCLAR+ R
Sbjct: 435 FGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDNCLARITR 486
BLAST of Cp4.1LG05g02130 vs. ExPASy Swiss-Prot
Match:
Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 446.8 bits (1148), Expect = 3.1e-124
Identity = 215/464 (46.34%), Postives = 309/464 (66.59%), Query Frame = 0
Query: 30 WRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKTGMYKTHLF 89
W RR LPPGDMGWPL+G + FL+AF+SGRPDSFI F ++G+ G+Y+ +F
Sbjct: 33 WTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFKSGRPDSFIDSFARRFGRAGLYRAFMF 92
Query: 90 GSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNSVPKPEHRRLRRLIASLIS 149
SP+++ T PE C+ VLM+D+ FV G+ KAT L G K+ ++ EHRRLR+L A+ I+
Sbjct: 93 SSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRKLTAAPIN 152
Query: 150 GNEALSLYIGHVEGIVVTCLEEWGSM--KEPVEFLSEMKTVAFKVLLHIFIGANSAAFID 209
G +AL+ Y+G ++ VVT L W EFL+E++ + F++++ IF+G
Sbjct: 153 GFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGGADERTAA 212
Query: 210 KMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKR-ENLKNKVGENRK 269
++E+ Y + + G + A+D+PG + +A++AR L+ ++VL+E+R K G
Sbjct: 213 ELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGGKTAAGAAAP 272
Query: 270 KDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPETLRKAK 329
DM D L+ V DE G L D IID+L+ + AGHE+S H M A +FL ENPE L KAK
Sbjct: 273 VDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPEILAKAK 332
Query: 330 AEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDVNLNGY 389
AEQE I+ + P KGLT+++ ++M YLS+V+DETLR +++F R+ DV +NGY
Sbjct: 333 AEQEAIMRSIPPG--QKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFRQATRDVFVNGY 392
Query: 390 TIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSK 449
IPKGWK+ +W R+VHM+P++Y +P KFDPSRW+ P RAG+F+PFG G RLCPG DL+K
Sbjct: 393 LIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLGTRLCPGNDLAK 452
Query: 450 LEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARVVR 491
LEI++FLH+F+ G++L R NPNC +LP P P DNCLA++ R
Sbjct: 453 LEISVFLHHFLL-GYKLTRKNPNCRVRYLPHPRPVDNCLAKITR 493
BLAST of Cp4.1LG05g02130 vs. ExPASy Swiss-Prot
Match:
Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)
HSP 1 Score: 435.3 bits (1118), Expect = 9.4e-121
Identity = 214/484 (44.21%), Postives = 321/484 (66.32%), Query Frame = 0
Query: 9 LIGALAGVYVFLFGILRNLNQW--RFVTRHARRYNLPPGDMGWPLIGTLLPFLKAFRSGR 68
L+GA+A V + R + W ARR LPPG+MGWPL+G + FL+AF+SG+
Sbjct: 39 LVGAVA--LVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGK 98
Query: 69 PDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGS 128
PD+FI F ++G+TG+Y++ +F SP+V+VT E C+ VLM+D+ FV G+ KAT L G
Sbjct: 99 PDAFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGP 158
Query: 129 KALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMK 188
++ ++P EHRR+R+L A+ I+G +AL+ Y+ ++ V + L W VEFL+E++
Sbjct: 159 RSFVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELR 218
Query: 189 TVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIF 248
+ FK+++ IF+G A +E+ Y + + G + A+++PG + AL+AR L+ +
Sbjct: 219 RMTFKIIVQIFLGGADQATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVL 278
Query: 249 EKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAH 308
+ VL+E+R + K DM D L+E +DE G LDD IID+L+ + AGHE+S H
Sbjct: 279 QGVLDERRA-ARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGH 338
Query: 309 TIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRK 368
M A +FL ENP+ +AKAEQE I+ + P + +GLT+++ ++MEYLS+V+DETLR
Sbjct: 339 ITMWATVFLQENPDMFARAKAEQEAIMRSIP--SSQRGLTLRDFRKMEYLSQVIDETLRL 398
Query: 369 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRA 428
+++F R+ DV +NGY IPKGWK+ +W R+VHM+P++Y +P KFDPSRW+ RA
Sbjct: 399 VNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRA 458
Query: 429 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLA 488
G+F+ FG G RLCPG DL+KLEI++FLH+F+ G++L R NP C +LP P P DNCLA
Sbjct: 459 GTFLAFGLGARLCPGNDLAKLEISVFLHHFLL-GYKLARTNPRCRVRYLPHPRPVDNCLA 516
Query: 489 RVVR 491
++ R
Sbjct: 519 KITR 516
BLAST of Cp4.1LG05g02130 vs. NCBI nr
Match:
XP_023534581.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0
Query: 1 MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK 60
MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK
Sbjct: 1 MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK 60
Query: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT
Sbjct: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
Query: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE
Sbjct: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
Query: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN
Sbjct: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
Query: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG
Sbjct: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
Query: 301 HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM
Sbjct: 301 HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
Query: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD
Sbjct: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
Query: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP
Sbjct: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
Query: 481 TDNCLARVVRDS 492
TDNCLARVVRDS
Sbjct: 481 TDNCLARVVRDS 492
BLAST of Cp4.1LG05g02130 vs. NCBI nr
Match:
XP_022931256.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])
HSP 1 Score: 988 bits (2555), Expect = 0.0
Identity = 487/492 (98.98%), Postives = 488/492 (99.19%), Query Frame = 0
Query: 1 MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK 60
MDMDI LCLIGALAGVYVFLFGILRNLNQWRFVTRH RRYNLPPGDMGWPLIGTLLPFLK
Sbjct: 1 MDMDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLK 60
Query: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT
Sbjct: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
Query: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYI HVEGIVVTCLEEWGSMKEPVE
Sbjct: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVE 180
Query: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFH+GFMSTAVDIPGTTFSRALKARN
Sbjct: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARN 240
Query: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG
Sbjct: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
Query: 301 HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
HETSAHTIMRAIMFLSENPETL KAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM
Sbjct: 301 HETSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
Query: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD
Sbjct: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
Query: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP
Sbjct: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
Query: 481 TDNCLARVVRDS 492
TDNCLARVVRDS
Sbjct: 481 TDNCLARVVRDS 492
BLAST of Cp4.1LG05g02130 vs. NCBI nr
Match:
XP_022995340.1 (beta-amyrin 11-oxidase-like [Cucurbita maxima])
HSP 1 Score: 988 bits (2554), Expect = 0.0
Identity = 486/492 (98.78%), Postives = 489/492 (99.39%), Query Frame = 0
Query: 1 MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK 60
MDMDI LCLIGALAGVYVF+FGILRNLNQWRFVTRH RRYNLPPGDMGWPLIGTLLPFLK
Sbjct: 1 MDMDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLK 60
Query: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT
Sbjct: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
Query: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE
Sbjct: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
Query: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN
Sbjct: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
Query: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG
Sbjct: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
Query: 301 HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
HETSAHTIMRAIMFLSENPETL KAKAEQE+IVNARPADAQHKGLTMKEIKQMEYLSKVM
Sbjct: 301 HETSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
Query: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPH+FDPSRWD
Sbjct: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWD 420
Query: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP
Sbjct: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
Query: 481 TDNCLARVVRDS 492
TDNCLARVVRDS
Sbjct: 481 TDNCLARVVRDS 492
BLAST of Cp4.1LG05g02130 vs. NCBI nr
Match:
KAG7036178.1 (Beta-amyrin 11-oxidase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 981 bits (2536), Expect = 0.0
Identity = 483/490 (98.57%), Postives = 485/490 (98.98%), Query Frame = 0
Query: 3 MDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAF 62
MDI +CLIGALAGVYVFLFGI RNLNQWRFVTRH RRYNLPPGDMGWPLIGTLLPFLKAF
Sbjct: 1 MDIWVCLIGALAGVYVFLFGIFRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLKAF 60
Query: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
RSGRPDSFIHHFTSKYGKTGMY+THLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI
Sbjct: 61 RSGRPDSFIHHFTSKYGKTGMYRTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 120
Query: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL
Sbjct: 121 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 180
Query: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 240
Query: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
IRIFEKVLEEKRENLKNKV ENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFEKVLEEKRENLKNKVEENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 300
Query: 303 TSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
TSAHTIMRAIMFLSENPETL KAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE
Sbjct: 301 TSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 360
Query: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 420
Query: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 480
Query: 483 NCLARVVRDS 492
NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Cp4.1LG05g02130 vs. NCBI nr
Match:
XP_008465946.1 (PREDICTED: beta-amyrin 11-oxidase-like [Cucumis melo] >KAA0038547.1 beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])
HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 444/486 (91.36%), Postives = 463/486 (95.27%), Query Frame = 0
Query: 7 LCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAFRSGR 66
L L+GAL Y+F+F ILRNLNQW FVTRH RRYNLPPGDMGWPLIGTLLPFL+AFRSGR
Sbjct: 6 LSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFRSGR 65
Query: 67 PDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGS 126
PDSFIH F KYGK GMYKT+LFGSPSVIVTVPEVCRHVLMNDEQF FGYSKATRILTGS
Sbjct: 66 PDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRILTGS 125
Query: 127 KALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMK 186
KALN+VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEW SMK+PVEFLSEMK
Sbjct: 126 KALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLSEMK 185
Query: 187 TVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIF 246
TVAFKVLLHIFIGAN+AAFID+MEKLYNDFHLGFMS+ VD+PGTTFSRALKARNELI+IF
Sbjct: 186 TVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELIKIF 245
Query: 247 EKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAH 306
E VL+EKRENLK KV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHETSAH
Sbjct: 246 ENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAH 305
Query: 307 TIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRK 366
+IMRAIMFLSENPETL+KAKAEQEQIV ARPADAQHKGLTMKEIKQMEYL KV+DETLRK
Sbjct: 306 SIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDETLRK 365
Query: 367 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRA 426
TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRW+NPKRR
Sbjct: 366 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPKRRG 425
Query: 427 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLA 486
GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLP PTDNCLA
Sbjct: 426 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLA 485
Query: 487 RVVRDS 492
RVVRDS
Sbjct: 486 RVVRDS 491
BLAST of Cp4.1LG05g02130 vs. ExPASy TrEMBL
Match:
A0A6J1ET68 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 SV=1)
HSP 1 Score: 988 bits (2555), Expect = 0.0
Identity = 487/492 (98.98%), Postives = 488/492 (99.19%), Query Frame = 0
Query: 1 MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK 60
MDMDI LCLIGALAGVYVFLFGILRNLNQWRFVTRH RRYNLPPGDMGWPLIGTLLPFLK
Sbjct: 1 MDMDIWLCLIGALAGVYVFLFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLK 60
Query: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT
Sbjct: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
Query: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYI HVEGIVVTCLEEWGSMKEPVE
Sbjct: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIEHVEGIVVTCLEEWGSMKEPVE 180
Query: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFH+GFMSTAVDIPGTTFSRALKARN
Sbjct: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHMGFMSTAVDIPGTTFSRALKARN 240
Query: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG
Sbjct: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
Query: 301 HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
HETSAHTIMRAIMFLSENPETL KAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM
Sbjct: 301 HETSAHTIMRAIMFLSENPETLLKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
Query: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD
Sbjct: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
Query: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP
Sbjct: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
Query: 481 TDNCLARVVRDS 492
TDNCLARVVRDS
Sbjct: 481 TDNCLARVVRDS 492
BLAST of Cp4.1LG05g02130 vs. ExPASy TrEMBL
Match:
A0A6J1K3T5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=1)
HSP 1 Score: 988 bits (2554), Expect = 0.0
Identity = 486/492 (98.78%), Postives = 489/492 (99.39%), Query Frame = 0
Query: 1 MDMDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLK 60
MDMDI LCLIGALAGVYVF+FGILRNLNQWRFVTRH RRYNLPPGDMGWPLIGTLLPFLK
Sbjct: 1 MDMDIWLCLIGALAGVYVFVFGILRNLNQWRFVTRHGRRYNLPPGDMGWPLIGTLLPFLK 60
Query: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT
Sbjct: 61 AFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKAT 120
Query: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE
Sbjct: 121 RILTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVE 180
Query: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN
Sbjct: 181 FLSEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARN 240
Query: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG
Sbjct: 241 ELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAG 300
Query: 301 HETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
HETSAHTIMRAIMFLSENPETL KAKAEQE+IVNARPADAQHKGLTMKEIKQMEYLSKVM
Sbjct: 301 HETSAHTIMRAIMFLSENPETLLKAKAEQEKIVNARPADAQHKGLTMKEIKQMEYLSKVM 360
Query: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWD 420
DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPH+FDPSRWD
Sbjct: 361 DETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHQFDPSRWD 420
Query: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP
Sbjct: 421 NPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTP 480
Query: 481 TDNCLARVVRDS 492
TDNCLARVVRDS
Sbjct: 481 TDNCLARVVRDS 492
BLAST of Cp4.1LG05g02130 vs. ExPASy TrEMBL
Match:
A0A5A7TAG0 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00740 PE=3 SV=1)
HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 444/486 (91.36%), Postives = 463/486 (95.27%), Query Frame = 0
Query: 7 LCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAFRSGR 66
L L+GAL Y+F+F ILRNLNQW FVTRH RRYNLPPGDMGWPLIGTLLPFL+AFRSGR
Sbjct: 6 LSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFRSGR 65
Query: 67 PDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGS 126
PDSFIH F KYGK GMYKT+LFGSPSVIVTVPEVCRHVLMNDEQF FGYSKATRILTGS
Sbjct: 66 PDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRILTGS 125
Query: 127 KALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMK 186
KALN+VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEW SMK+PVEFLSEMK
Sbjct: 126 KALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLSEMK 185
Query: 187 TVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIF 246
TVAFKVLLHIFIGAN+AAFID+MEKLYNDFHLGFMS+ VD+PGTTFSRALKARNELI+IF
Sbjct: 186 TVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELIKIF 245
Query: 247 EKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAH 306
E VL+EKRENLK KV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHETSAH
Sbjct: 246 ENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAH 305
Query: 307 TIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRK 366
+IMRAIMFLSENPETL+KAKAEQEQIV ARPADAQHKGLTMKEIKQMEYL KV+DETLRK
Sbjct: 306 SIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDETLRK 365
Query: 367 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRA 426
TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRW+NPKRR
Sbjct: 366 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPKRRG 425
Query: 427 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLA 486
GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLP PTDNCLA
Sbjct: 426 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLA 485
Query: 487 RVVRDS 492
RVVRDS
Sbjct: 486 RVVRDS 491
BLAST of Cp4.1LG05g02130 vs. ExPASy TrEMBL
Match:
A0A1S3CQE4 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503517 PE=3 SV=1)
HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 444/486 (91.36%), Postives = 463/486 (95.27%), Query Frame = 0
Query: 7 LCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAFRSGR 66
L L+GAL Y+F+F ILRNLNQW FVTRH RRYNLPPGDMGWPLIGTLLPFL+AFRSGR
Sbjct: 6 LSLLGALVASYIFVFLILRNLNQWWFVTRHGRRYNLPPGDMGWPLIGTLLPFLQAFRSGR 65
Query: 67 PDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGS 126
PDSFIH F KYGK GMYKT+LFGSPSVIVTVPEVCRHVLMNDEQF FGYSKATRILTGS
Sbjct: 66 PDSFIHLFAYKYGKIGMYKTYLFGSPSVIVTVPEVCRHVLMNDEQFGFGYSKATRILTGS 125
Query: 127 KALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMK 186
KALN+VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEW SMK+PVEFLSEMK
Sbjct: 126 KALNTVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWASMKKPVEFLSEMK 185
Query: 187 TVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIF 246
TVAFKVLLHIFIGAN+AAFID+MEKLYNDFHLGFMS+ VD+PGTTFSRALKARNELI+IF
Sbjct: 186 TVAFKVLLHIFIGANTAAFIDRMEKLYNDFHLGFMSSPVDLPGTTFSRALKARNELIKIF 245
Query: 247 EKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAH 306
E VL+EKRENLK KV ENRKKDM DLLLEVRDEDG+GLDDGCIIDLLIGFFFAGHETSAH
Sbjct: 246 ENVLKEKRENLKKKVAENRKKDMTDLLLEVRDEDGQGLDDGCIIDLLIGFFFAGHETSAH 305
Query: 307 TIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRK 366
+IMRAIMFLSENPETL+KAKAEQEQIV ARPADAQHKGLTMKEIKQMEYL KV+DETLRK
Sbjct: 306 SIMRAIMFLSENPETLQKAKAEQEQIVKARPADAQHKGLTMKEIKQMEYLFKVIDETLRK 365
Query: 367 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRA 426
TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRW+NPKRR
Sbjct: 366 TSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPQKFDPSRWENPKRRG 425
Query: 427 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLA 486
GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLP PTDNCLA
Sbjct: 426 GSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPRPTDNCLA 485
Query: 487 RVVRDS 492
RVVRDS
Sbjct: 486 RVVRDS 491
BLAST of Cp4.1LG05g02130 vs. ExPASy TrEMBL
Match:
A0A6J1GM12 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 SV=1)
HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 434/490 (88.57%), Postives = 468/490 (95.51%), Query Frame = 0
Query: 3 MDISLCLIGALAGVYVFLFGILRNLNQWRFVTRHARRYNLPPGDMGWPLIGTLLPFLKAF 62
M++ + L+GAL G Y+ +FGILRNLNQWRFVTRH RYNLPPGDMGWPL+GTLLPFL+AF
Sbjct: 1 MEVWVWLVGALVGAYICVFGILRNLNQWRFVTRHGGRYNLPPGDMGWPLVGTLLPFLQAF 60
Query: 63 RSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRI 122
RSGRPDSFIHHF SKYGK GMYKT+LFGSPSVIV VPEVCRHVLMNDEQF FGYSKATRI
Sbjct: 61 RSGRPDSFIHHFASKYGKIGMYKTYLFGSPSVIVCVPEVCRHVLMNDEQFAFGYSKATRI 120
Query: 123 LTGSKALNSVPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFL 182
LTGSKALN+V +PEHRR+RRLIASLISGN+ALS+YIGHVEGIV+TCLEEW +MK+PVEFL
Sbjct: 121 LTGSKALNTVSRPEHRRIRRLIASLISGNDALSIYIGHVEGIVITCLEEWATMKKPVEFL 180
Query: 183 SEMKTVAFKVLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNEL 242
SEMKT+AFK+LLHIFIGAN+AAFIDKMEKLY DFHLGFMS+AVDIPGTTFSRALKARNEL
Sbjct: 181 SEMKTMAFKILLHIFIGANTAAFIDKMEKLYTDFHLGFMSSAVDIPGTTFSRALKARNEL 240
Query: 243 IRIFEKVLEEKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHE 302
IRIFE VL+EKRENLKNKV ENRKKDM DLLLEV+DE+G GLDDGCIIDLLIGFFFAGHE
Sbjct: 241 IRIFESVLKEKRENLKNKVEENRKKDMTDLLLEVKDEEGHGLDDGCIIDLLIGFFFAGHE 300
Query: 303 TSAHTIMRAIMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDE 362
TSAH+IMRAIMFLSENPETLRKAKAEQ+QIVNARP++ +H+GLTMKEIKQMEYLSKV+DE
Sbjct: 301 TSAHSIMRAIMFLSENPETLRKAKAEQDQIVNARPSNERHRGLTMKEIKQMEYLSKVIDE 360
Query: 363 TLRKTSLAFTLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNP 422
TLRKTSLAFTLSRET+ DVNLNGYTIPKGWKILVWTRAVHM+PEIYENP KFDPSRWDNP
Sbjct: 361 TLRKTSLAFTLSRETRSDVNLNGYTIPKGWKILVWTRAVHMDPEIYENPEKFDPSRWDNP 420
Query: 423 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTD 482
KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPEN+LPLP PTD
Sbjct: 421 KRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENYLPLPRPTD 480
Query: 483 NCLARVVRDS 492
NCLARVVRDS
Sbjct: 481 NCLARVVRDS 490
BLAST of Cp4.1LG05g02130 vs. TAIR 10
Match:
AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )
HSP 1 Score: 460.3 bits (1183), Expect = 1.9e-129
Identity = 227/470 (48.30%), Postives = 325/470 (69.15%), Query Frame = 0
Query: 23 ILRNLNQWRFVTR-HARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKT 82
++R +N W + + R+ LPPGD+GWP IG +L FL+AF++ PDSF +YG
Sbjct: 25 LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84
Query: 83 GMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNSVPKPEHRRLR 142
G+YK H+FG+PS+IVT + CR VL +D+ F G+ +T L G K+ + EH+RLR
Sbjct: 85 GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144
Query: 143 RLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKVLLHIFIGAN 202
RL A+ ++G+EALS YI ++E V+T L++W M E EFL+ ++ + F+++++IF+ +
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204
Query: 203 SAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKV 262
S +D +E+ Y + G + AV+IPG + RALKAR L+ F+ ++ E+R K +
Sbjct: 205 SENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQNI 264
Query: 263 GENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPET 322
N KKDM D LL V+DEDG+ LDD IID+L+ + AGHE+S HTIM A +FL E+PE
Sbjct: 265 LSN-KKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEV 324
Query: 323 LRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDV 382
L++AKAEQE I+ +RP KGL++KE ++ME+LS+V+DETLR + + T RE K DV
Sbjct: 325 LQRAKAEQEMILKSRPEG--QKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDV 384
Query: 383 NLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDN---PKRRAGSFIPFGAGMRL 442
+NGY IPKGWK+L W R VH++PE++ +P KFDP+RWDN PK AG+F+PFGAG L
Sbjct: 385 EMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPK--AGAFLPFGAGSHL 444
Query: 443 CPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARV 489
CPG DL+KLEI+IFLH+F+ ++++R NP CP +LP PTDNCLAR+
Sbjct: 445 CPGNDLAKLEISIFLHHFLL-KYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of Cp4.1LG05g02130 vs. TAIR 10
Match:
AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-127
Identity = 224/479 (46.76%), Postives = 327/479 (68.27%), Query Frame = 0
Query: 13 LAGVYVFLFGILRNLNQWRFVTR-HARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFI 72
+ G++V L +L+ +N W +V++ +++ LPPGD+GWP+IG + FL+AF++ P+SFI
Sbjct: 15 ILGLFV-LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFI 74
Query: 73 HHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNS 132
+ ++YG+TG+YK H+FG P V+VT PE CR VL +D+ F G+ K+T L G K+
Sbjct: 75 QSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVG 134
Query: 133 VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFK 192
+ EH+RLRRL ++ ++G EALS+YI +E V T LE+W M E +EFLS ++ + FK
Sbjct: 135 ISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFK 194
Query: 193 VLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLE 252
V+++IF+ + S +D +E+ Y + + G + +++PG + RALKAR +L+ F+ ++
Sbjct: 195 VIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVT 254
Query: 253 EKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRA 312
+R K + NR KDM D L++V+DE+G LDD IIDLL+ + AGHE+S H M A
Sbjct: 255 NRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWA 314
Query: 313 IMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAF 372
+ + E+P L+KAK EQE+IV R A + LT+KE ++M YLS+V+DETLR + +
Sbjct: 315 TILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDETLRVITFSL 374
Query: 373 TLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIP 432
T RE K DV ++GY IPKGWK+L W R VH++PEIY +P KFDPSRW+ +AG+F+P
Sbjct: 375 TAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLP 434
Query: 433 FGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARVVR 491
FG G LCPG DL+KLEI+IFLH+F+ +R+ER NP CP LP P DNCLAR+ R
Sbjct: 435 FGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDNCLARITR 486
BLAST of Cp4.1LG05g02130 vs. TAIR 10
Match:
AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-127
Identity = 224/479 (46.76%), Postives = 327/479 (68.27%), Query Frame = 0
Query: 13 LAGVYVFLFGILRNLNQWRFVTR-HARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFI 72
+ G++V L +L+ +N W +V++ +++ LPPGD+GWP+IG + FL+AF++ P+SFI
Sbjct: 15 ILGLFV-LKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPESFI 74
Query: 73 HHFTSKYGKTGMYKTHLFGSPSVIVTVPEVCRHVLMNDEQFVFGYSKATRILTGSKALNS 132
+ ++YG+TG+YK H+FG P V+VT PE CR VL +D+ F G+ K+T L G K+
Sbjct: 75 QSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVG 134
Query: 133 VPKPEHRRLRRLIASLISGNEALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFK 192
+ EH+RLRRL ++ ++G EALS+YI +E V T LE+W M E +EFLS ++ + FK
Sbjct: 135 ISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFK 194
Query: 193 VLLHIFIGANSAAFIDKMEKLYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLE 252
V+++IF+ + S +D +E+ Y + + G + +++PG + RALKAR +L+ F+ ++
Sbjct: 195 VIMYIFLSSESEHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQSIVT 254
Query: 253 EKRENLKNKVGENRKKDMADLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRA 312
+R K + NR KDM D L++V+DE+G LDD IIDLL+ + AGHE+S H M A
Sbjct: 255 NRRNQRKQNISSNR-KDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWA 314
Query: 313 IMFLSENPETLRKAKAEQEQIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAF 372
+ + E+P L+KAK EQE+IV R A + LT+KE ++M YLS+V+DETLR + +
Sbjct: 315 TILMQEHPMILQKAKEEQERIVKKR---APGQKLTLKETREMVYLSQVIDETLRVITFSL 374
Query: 373 TLSRETKVDVNLNGYTIPKGWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIP 432
T RE K DV ++GY IPKGWK+L W R VH++PEIY +P KFDPSRW+ +AG+F+P
Sbjct: 375 TAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLP 434
Query: 433 FGAGMRLCPGIDLSKLEIAIFLHYFIHGGFRLERVNPNCPENHLPLPTPTDNCLARVVR 491
FG G LCPG DL+KLEI+IFLH+F+ +R+ER NP CP LP P DNCLAR+ R
Sbjct: 435 FGLGSHLCPGNDLAKLEISIFLHHFLL-KYRVERSNPGCPVMFLPHNRPKDNCLARITR 486
BLAST of Cp4.1LG05g02130 vs. TAIR 10
Match:
AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )
HSP 1 Score: 237.7 bits (605), Expect = 2.1e-62
Identity = 135/427 (31.62%), Postives = 226/427 (52.93%), Query Frame = 0
Query: 33 VTRHARRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSP 92
VT + P G +GWP+IG + F+ + S RP+SF+ YG+ ++K+H+FG+
Sbjct: 42 VTSQSHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGR--VFKSHIFGTA 101
Query: 93 SVIVTVPEVCRHVLMNDE-QFVFGYSKATRILTGSKALNSVPKPEHRRLRRLIASLISGN 152
+++ T EV R VL +D FV Y K R L G ++ + HRR L+ S +
Sbjct: 102 TIVSTDAEVNRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSP 161
Query: 153 EALSLYIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKVLLHIFIGANSAAFIDKMEK 212
+ + + + ++ W S +PV KTVAFKVL I ++++++
Sbjct: 162 LLKAQIVRDMHKFLSESMDLW-SEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKR 221
Query: 213 LYNDFHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKVGEN-RKKDMA 272
+ +F G MS ++ PGT R+L+A+ +++ E+++E K KNK ++ KD+
Sbjct: 222 EFENFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVV 281
Query: 273 DLLLEVRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPETLRKAKAEQE 332
D+LL+ + E L I + +I GH++ I A+ FLS++P L E
Sbjct: 282 DVLLK---DSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENM 341
Query: 333 QIVNARPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDVNLNGYTIPK 392
++ + + + + L + + + KV+ ETLR ++ + R+ DV + GY IPK
Sbjct: 342 KLKSLK--ELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPK 401
Query: 393 GWKILVWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIA 452
GW L + R+VH++ YE+P+KF+P RW SF PFG G RLCPG+DL++LE +
Sbjct: 402 GWCFLAYLRSVHLDKLYYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETS 460
Query: 453 IFLHYFI 458
+FLH+ +
Sbjct: 462 VFLHHLV 460
BLAST of Cp4.1LG05g02130 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 232.3 bits (591), Expect = 8.6e-61
Identity = 150/453 (33.11%), Postives = 236/453 (52.10%), Query Frame = 0
Query: 38 RRYNLPPGDMGWPLIGTLLPFLKAFRSGRPDSFIHHFTSKYGKTGMYKTHLFGSPSVIVT 97
+R LPPG MG P IG L + P+SF +KYG ++KTH+ G P V+++
Sbjct: 45 QRLRLPPGSMGLPYIGETL----RLYTENPNSFFATRQNKYG--DIFKTHILGCPCVMIS 104
Query: 98 VPEVCRHVLMNDEQ-FVFGYSKATRILTGSKALNSVPKPEHRRLRRLIASLISGNEALSL 157
PE R VL++ F Y + + G +AL P H L+RL+ S + AL
Sbjct: 105 SPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPS-ALRP 164
Query: 158 YIGHVEGIVVTCLEEWGSMKEPVEFLSEMKTVAFKV-LLHIFIGANSAAFIDKMEKLYND 217
+ H+E +V+ L W S K + L MK AF V ++ F ID ++ LY
Sbjct: 165 TVSHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQR 224
Query: 218 FHLGFMSTAVDIPGTTFSRALKARNELIRIFEKVLEEKRENLKNKVGENRKKDMADLLLE 277
G+ S +D+PGT F +++KAR EL KV+E++REN + + G + +LL
Sbjct: 225 LERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGG------LLGVLLG 284
Query: 278 VRDEDGEGLDDGCIIDLLIGFFFAGHETSAHTIMRAIMFLSENPETLRKAKAEQEQIVNA 337
+D+ GL D I D +IG FA +T+A + + +L ++P L++ EQ I
Sbjct: 285 AKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSI--R 344
Query: 338 RPADAQHKGLTMKEIKQMEYLSKVMDETLRKTSLAFTLSRETKVDVNLNGYTIPKGWKIL 397
+ +++ ++ ++ ++M ++V+ ETLR S+ RE DV +GY IPKGWK+L
Sbjct: 345 QKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVL 404
Query: 398 VWTRAVHMNPEIYENPHKFDPSRWDNPKRRAGSFIPFGAGMRLCPGIDLSKLEIAIFLHY 457
R +H + E + +P KFDPSR++ + +++PFG G+ CPG +L+KLE+ I LH+
Sbjct: 405 PLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHH 464
Query: 458 FIHGGFRLERVNPNCPENHLPLPTPTDNCLARV 489
+ FR E + + P P P RV
Sbjct: 465 -LTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRV 479
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
B5BSX1 | 1.2e-139 | 50.10 | Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1 | [more] |
O23051 | 2.7e-128 | 48.30 | Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1 | [more] |
Q9C5Y2 | 1.5e-126 | 46.76 | Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2 | [more] |
Q9AXH9 | 3.1e-124 | 46.34 | Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1 | [more] |
Q43246 | 9.4e-121 | 44.21 | Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1ET68 | 0.0 | 98.98 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111437486 PE=3 S... | [more] |
A0A6J1K3T5 | 0.0 | 98.78 | beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111490917 PE=3 SV=... | [more] |
A0A5A7TAG0 | 0.0 | 91.36 | Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... | [more] |
A0A1S3CQE4 | 0.0 | 91.36 | beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503517 PE=3 SV=1 | [more] |
A0A6J1GM12 | 0.0 | 88.57 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455188 PE=3 S... | [more] |