Cp4.1LG05g01970 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g01970
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	Cp4.1LG05: 1124801 .. 1125346 (-)
RNA-Seq Expression	Cp4.1LG05g01970
Synteny	Cp4.1LG05g01970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAACCCATTTTCCAGCATCGATTCTAGTGCTTGTTTCTCTTCTAACCCTCTTCCAATTTGGGTTCTCTTTGGACATTGTTCAACGCTCCTGTAAACTAGCTGCAAAAAGAGACCCATACGTTGATTACAAGCTCTGTGTTAAGAGCCTACGAGCCAACCCCAGTTCAAAAGATGCAACGTTCAAGGATTTGGTCCTGATTTCGATCGAGCAATCGAAGGCTAATGCGACGGCAATTGGGTCGGAGATTACGGGGCTCTTAAAGGGCGGTGGGAAATGGGGGAAGTATTCGACGAGTTGTTTGGAGAGCTGTTTGGAGGTTTATTCTGAGGCTGTTTCGGATTTGAAATTGGGTTTGGTTGCTGTCAAAATGGGGGATTATGAAACTGCAAATACAGAGGTGAGTGCCGCCATGGATGCACCTTTGACTTGTGAAAATGGGTATAATGAGAAGGATGGGGAAGTTTCTCCTTTGACAGAGAAGAACGGTGGGTTCTTTCAGCTGACTGCAATTTCTCTGGCTTTTATCAGTTTGTGTCAGTGA

mRNA sequence

ATGAAAACCCATTTTCCAGCATCGATTCTAGTGCTTGTTTCTCTTCTAACCCTCTTCCAATTTGGGTTCTCTTTGGACATTGTTCAACGCTCCTGTAAACTAGCTGCAAAAAGAGACCCATACGTTGATTACAAGCTCTGTGTTAAGAGCCTACGAGCCAACCCCAGTTCAAAAGATGCAACGTTCAAGGATTTGGTCCTGATTTCGATCGAGCAATCGAAGGCTAATGCGACGGCAATTGGGTCGGAGATTACGGGGCTCTTAAAGGGCGGTGGGAAATGGGGGAAGTATTCGACGAGTTGTTTGGAGAGCTGTTTGGAGGTTTATTCTGAGGCTGTTTCGGATTTGAAATTGGGTTTGGTTGCTGTCAAAATGGGGGATTATGAAACTGCAAATACAGAGGTGAGTGCCGCCATGGATGCACCTTTGACTTGTGAAAATGGGTATAATGAGAAGGATGGGGAAGTTTCTCCTTTGACAGAGAAGAACGGTGGGTTCTTTCAGCTGACTGCAATTTCTCTGGCTTTTATCAGTTTGTGTCAGTGA

Coding sequence (CDS)

ATGAAAACCCATTTTCCAGCATCGATTCTAGTGCTTGTTTCTCTTCTAACCCTCTTCCAATTTGGGTTCTCTTTGGACATTGTTCAACGCTCCTGTAAACTAGCTGCAAAAAGAGACCCATACGTTGATTACAAGCTCTGTGTTAAGAGCCTACGAGCCAACCCCAGTTCAAAAGATGCAACGTTCAAGGATTTGGTCCTGATTTCGATCGAGCAATCGAAGGCTAATGCGACGGCAATTGGGTCGGAGATTACGGGGCTCTTAAAGGGCGGTGGGAAATGGGGGAAGTATTCGACGAGTTGTTTGGAGAGCTGTTTGGAGGTTTATTCTGAGGCTGTTTCGGATTTGAAATTGGGTTTGGTTGCTGTCAAAATGGGGGATTATGAAACTGCAAATACAGAGGTGAGTGCCGCCATGGATGCACCTTTGACTTGTGAAAATGGGTATAATGAGAAGGATGGGGAAGTTTCTCCTTTGACAGAGAAGAACGGTGGGTTCTTTCAGCTGACTGCAATTTCTCTGGCTTTTATCAGTTTGTGTCAGTGA

Protein sequence

MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ

Homology

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-24
Identity = 72/179 (40.22%), Postives = 102/179 (56.98%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           MK  F   I    +LL L Q   S DIVQ +CK  A+R P V+Y  CVKSL A+P S  A
Sbjct: 1   MKLSFSLCIF-FFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120
             + L +IS   +  + + I + I  +LK   K        L  C+ +Y++A S ++  +
Sbjct: 61  DLQGLGVISANLAIQHGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQEAI 120

Query: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
              K  DY +AN ++SAA+D  +TCE+G+ EK G VSP+T++N  + QLTAISLA   L
Sbjct: 121 ADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 5.7e-23
Identity = 64/159 (40.25%), Postives = 92/159 (57.86%), Query Frame = 0


Query: 21  FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI 80
           F  S DIVQ +CK  A+R P V+Y  CVKSL A+P S  A  + L +IS   +    + I
Sbjct: 11  FLISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKI 70

Query: 81  GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD 140
            + I  +LK   K        L  C+ +Y++A S ++  +   K  DY +AN ++SAA+D
Sbjct: 71  QTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALD 130

Query: 141 APLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
             +TCE+G+ EK G  SP+T++N  + QLTAISLA   L
Sbjct: 131 DSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.6e-17
Identity = 62/175 (35.43%), Postives = 93/175 (53.14%), Query Frame = 0


Query: 10  LVLVSLLTLFQFGFSL--DIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLV 69
           LV + + +LF  GF+    ++Q SCK A  +DP + Y  CV SL  +P SK A T + LV
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLV 63

Query: 70  LISIEQSKANATAIGSEITGLLKGGGKWGKYS--TSCLESCLEVYSEAVSDLKLGLVAVK 129
           L S + + A  T     + G++    K  +Y      L+ CL  Y++A  DL   L  +K
Sbjct: 64  LASTKTAAAKIT----NLKGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIK 123

Query: 130 MGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
             DY+ AN  +SAA+D P  CE+ + E   + SP+T +N   F+   I LAF ++
Sbjct: 124 SRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.8e-06
Identity = 44/158 (27.85%), Postives = 70/158 (44.30%), Query Frame = 0


Query: 11  VLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 70
           VL+S L  F    SL+      K    +    +   C   +++NP +  A  + L  I+ 
Sbjct: 9   VLMSSLMFFVMTGSLNAQVADIKAICGKAK--NQSFCTSYMKSNPKTSGADLQTLANITF 68

Query: 71  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 130
             ++ +A+    +I  L+K              SC++ Y  A+S L     ++  GD + 
Sbjct: 69  GSAQTSASEGFRKIQSLVKTA--TNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKG 128

Query: 131 ANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQ 169
            N +VSAAM+ P TCE   +  D +V P   KN G FQ
Sbjct: 129 LNIKVSAAMEGPSTCEQ--DMADFKVDPSAVKNSGDFQ 160

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.9e-04
Identity = 35/144 (24.31%), Postives = 60/144 (41.67%), Query Frame = 0


Query: 44  YKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYST---- 103
           Y +CV +L A+PSS  A  + L  I++  + ANA+        L  G       +     
Sbjct: 72  YDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALANGTAPLAAAAAGDGT 131

Query: 104 --SCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVS 163
             + L +C   Y +A   L     ++   DY+ A   VSA  + P  C+  +  +     
Sbjct: 132 VQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYPQVCKALFRRQRPGAY 191

Query: 164 P--LTEKNGGFFQLTAISLAFISL 180
           P  L  +     +L +++L  I+L
Sbjct: 192 PAELAAREEALNKLCSVALDIIAL 215

[Close]

BLAST of Cp4.1LG05g01970 vs. NCBI nr
Match: XP_023534693.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355 bits (911), Expect = 2.10e-123
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA
Sbjct: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120
           TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL
Sbjct: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120

Query: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180
           VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC
Sbjct: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180

[Close]

BLAST of Cp4.1LG05g01970 vs. NCBI nr
Match: XP_022995887.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 352 bits (902), Expect = 4.96e-122
Identity = 179/181 (98.90%), Postives = 180/181 (99.45%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           MKTHFPASILVL SLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA
Sbjct: 1   MKTHFPASILVLFSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120
           TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYS+SCLESCLEVYSEAVSDLKLGL
Sbjct: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSSSCLESCLEVYSEAVSDLKLGL 120

Query: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180
           VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC
Sbjct: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180

[Close]

BLAST of Cp4.1LG05g01970 vs. NCBI nr
Match: XP_022958457.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 342 bits (878), Expect = 2.26e-118
Identity = 174/181 (96.13%), Postives = 177/181 (97.79%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           MKTHF  SIL+L SLLTLFQFGF LDIVQRSCKLAAKRDPYVDYKLCV+SLRANPSSKDA
Sbjct: 1   MKTHFSTSILMLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120
           TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL
Sbjct: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120

Query: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180
           VAVKMGDYETANTEVSAAMDAPLTCE+GYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC
Sbjct: 121 VAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180

[Close]

BLAST of Cp4.1LG05g01970 vs. NCBI nr
Match: KAG6606222.1 (hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036169.1 hypothetical protein SDJN02_02970, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 328 bits (841), Expect = 6.98e-113
Identity = 167/170 (98.24%), Postives = 168/170 (98.82%), Query Frame = 0


Query: 12  LVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIE 71
           L SLLTLFQFGF LDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIE
Sbjct: 2   LFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIE 61

Query: 72  QSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETA 131
           QSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETA
Sbjct: 62  QSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETA 121

Query: 132 NTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 181
           NTEVSAAMDAPLTCE+GYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ
Sbjct: 122 NTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLCQ 171

[Close]

BLAST of Cp4.1LG05g01970 vs. NCBI nr
Match: XP_023523101.1 (putative invertase inhibitor, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 294 bits (753), Expect = 7.93e-100
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 21  FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI 80
           FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI
Sbjct: 1   FGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISIEQSKANATAI 60

Query: 81  GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD 140
           GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD
Sbjct: 61  GSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTEVSAAMD 120

Query: 141 APLTCENGYNEKDGEVSPLTEKNGGFF 167
           APLTCENGYNEKDGEVSPLTEKNGGFF
Sbjct: 121 APLTCENGYNEKDGEVSPLTEKNGGFF 147

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy TrEMBL
Match: A0A6J1K084 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111491280 PE=4 SV=1)

HSP 1 Score: 352 bits (902), Expect = 2.40e-122
Identity = 179/181 (98.90%), Postives = 180/181 (99.45%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           MKTHFPASILVL SLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA
Sbjct: 1   MKTHFPASILVLFSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120
           TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYS+SCLESCLEVYSEAVSDLKLGL
Sbjct: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSSSCLESCLEVYSEAVSDLKLGL 120

Query: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180
           VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC
Sbjct: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy TrEMBL
Match: A0A6J1H1W1 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 SV=1)

HSP 1 Score: 342 bits (878), Expect = 1.10e-118
Identity = 174/181 (96.13%), Postives = 177/181 (97.79%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           MKTHF  SIL+L SLLTLFQFGF LDIVQRSCKLAAKRDPYVDYKLCV+SLRANPSSKDA
Sbjct: 1   MKTHFSTSILMLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120
           TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL
Sbjct: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGL 120

Query: 121 VAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180
           VAVKMGDYETANTEVSAAMDAPLTCE+GYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC
Sbjct: 121 VAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 180

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy TrEMBL
Match: A0A6J1ICI2 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV=1)

HSP 1 Score: 243 bits (620), Expect = 2.46e-79
Identity = 128/173 (73.99%), Postives = 146/173 (84.39%), Query Frame = 0


Query: 9   ILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLI 68
           +L+L+SL T F   FSLDI+QRSCK AAK DPYVDYKLCV++LRANP+SK ATFK+LVLI
Sbjct: 10  LLLLLSLPTPFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPNSKAATFKELVLI 69

Query: 69  SIEQSKANATAIGSEITGLLKGGGKWGK--YSTSCLESCLEVYSEAVSDLKLGLVAVKMG 128
           SI QSKANAT IGSEI+ LLKG  +WGK  YS  CL+ CLE+YSEAVS L+L L AVKMG
Sbjct: 70  SITQSKANATEIGSEISELLKGSERWGKGKYSLGCLKGCLEIYSEAVSGLELALGAVKMG 129

Query: 129 DYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 179
           DYETAN +VSAAMDA L+CE+GY EKDGEVSPLTE N GFFQL AISLAFI++
Sbjct: 130 DYETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQLNAISLAFINM 182

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy TrEMBL
Match: A0A0A0LHE9 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 SV=1)

HSP 1 Score: 237 bits (604), Expect = 6.05e-77
Identity = 123/181 (67.96%), Postives = 147/181 (81.22%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           M+     SIL L+ + TL Q  + LDIVQ SCKLAAK DPYVDYKLCV++L+A+P+SKDA
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPNSKDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGG-KWGKYSTSCLESCLEVYSEAVSDLKLG 120
            FKDLV+ISI QSKANAT IGSEI+ L+K    KWG+YS +CL+SCLE+YSEAVSDL+  
Sbjct: 61  EFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEKA 120

Query: 121 LVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
           L  +KM DYETA T VSAAMDAP++CE+GY EKDGEVSPL+E N GFFQL AISLAFI++
Sbjct: 121 LKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQLVAISLAFINM 180

[Close]

BLAST of Cp4.1LG05g01970 vs. ExPASy TrEMBL
Match: A0A5A7TAE5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004030 PE=4 SV=1)

HSP 1 Score: 233 bits (595), Expect = 1.42e-75
Identity = 122/181 (67.40%), Postives = 144/181 (79.56%), Query Frame = 0


Query: 1   MKTHFPASILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA 60
           M+     SIL L+ + TL Q    LDIVQ  CKLAAK DPYVDYKLCV++L+ANP+S+DA
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPNSEDA 60

Query: 61  TFKDLVLISIEQSKANATAIGSEITGLLKGGG-KWGKYSTSCLESCLEVYSEAVSDLKLG 120
            FKDLV+ISI Q+KANAT I SEI+ L+K    KWGKYS +CL+SCLE+YSEAVSDLK  
Sbjct: 61  DFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKKA 120

Query: 121 LVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
           L  +KM DYETA T VSAAMDAP++CE+GY EKDGEVSPL+E N GFFQL AISLAFI++
Sbjct: 121 LRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQLVAISLAFINM 180

[Close]

BLAST of Cp4.1LG05g01970 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 61/154 (39.61%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 16  LTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKD-LVLISIEQSK 75
           L L  F  +  ++Q  CK AA ++P + Y  CVKSL  NP SK A   D LV++S + + 
Sbjct: 12  LLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLSTKNAV 71

Query: 76  ANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYETANTE 135
           +  T++   +  +LK   ++  YS   L  CLE+YS+A + LK  L  +K  DY+TAN  
Sbjct: 72  SKTTSMKGIVDKILK-ENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRDYKTANVV 131

Query: 136 VSAAMDAPLTCENGYNE-KDGEVSPLTEKNGGFF 168
           +SAAM AP +CE G+ E K    SP T+ N   F
Sbjct: 132 ISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Cp4.1LG05g01970 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 1.4e-21
Identity = 61/173 (35.26%), Postives = 91/173 (52.60%), Query Frame = 0


Query: 12  LVSLLTLFQFGFSL----DIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLV 71
           L+ L+T F     L     +++ SCK A    P   Y LCV SL  NP +K A     LV
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 72  LISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMG 131
           + S + +   AT +   +  ++K G K  K +   L  CL++Y++A+  L   L  VK  
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIK-GKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSR 123

Query: 132 DYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
           +Y T  T +SAAMD P TCE G+ E+    SP+T++N   +Q+  I LAF ++
Sbjct: 124 NYPTVKTVLSAAMDTPSTCETGFKERKAP-SPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of Cp4.1LG05g01970 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.2e-21
Identity = 63/175 (36.00%), Postives = 97/175 (55.43%), Query Frame = 0


Query: 8   SILVLVSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLV 67
           S+ +++  L L  F  +  +++ SCK A  +DP + Y  CVKSL  NP SK A + + LV
Sbjct: 4   SLYLVMFFLLLNCFATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLV 63

Query: 68  LISIEQSKANATAIGSEITGLLKGGGKWGKYSTS-CLESCLEVYSEAVSDLKLGLVAVKM 127
            +S + + +  T++   +  +LK      KY     L  CLE+Y+EA+  L   L  VK 
Sbjct: 64  FVSTKNAVSKTTSLKGMVDKILKE----DKYEVERPLLDCLELYTEAIDSLNQSLDTVKS 123

Query: 128 GDYETANTEVSAAMDAPLTCENGYNEKDGEV-SPLTEKNGGFFQLTAISLAFISL 180
            DY+TA   +SAAMDAP +CE  + ++   V SP T++N   F +  I +AF S+
Sbjct: 124 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Cp4.1LG05g01970 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 2.5e-18
Identity = 62/175 (35.43%), Postives = 93/175 (53.14%), Query Frame = 0


Query: 10  LVLVSLLTLFQFGFSL--DIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLV 69
           LV + + +LF  GF+    ++Q SCK A  +DP + Y  CV SL  +P SK A T + LV
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLV 63

Query: 70  LISIEQSKANATAIGSEITGLLKGGGKWGKYS--TSCLESCLEVYSEAVSDLKLGLVAVK 129
           L S + + A  T     + G++    K  +Y      L+ CL  Y++A  DL   L  +K
Sbjct: 64  LASTKTAAAKIT----NLKGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIK 123

Query: 130 MGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
             DY+ AN  +SAA+D P  CE+ + E   + SP+T +N   F+   I LAF ++
Sbjct: 124 SRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Cp4.1LG05g01970 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 7.4e-18
Identity = 61/175 (34.86%), Postives = 94/175 (53.71%), Query Frame = 0


Query: 10  LVLVSLLTLFQFGFSL--DIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDA-TFKDLV 69
           LV + + +LF  GF+    ++Q SCK A  +DP   Y  CV+SL  +P SK A T +DL 
Sbjct: 4   LVSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTLEDLA 63

Query: 70  LISIEQSKANATAIGSEITGLLKGGGKWGKYS--TSCLESCLEVYSEAVSDLKLGLVAVK 129
           L S +   A  T     + G++    K  +Y      L+ CL  Y +A   LK  L  +K
Sbjct: 64  LASTKNVAAKIT----NLKGIVAQDLKDQRYQDIVEDLKLCLGFYKDANDSLKTALANIK 123

Query: 130 MGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFISL 180
             DY+ AN+ +SAA++AP  CE+ + E + + SP+T +N   ++   I LAF ++
Sbjct: 124 SRDYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTM 173

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g01970 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	2.3e-24	40.22	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	5.7e-23	40.25	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	3.6e-17	35.43	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q9LUV1	4.8e-06	27.85	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q0J8J8	5.9e-04	24.31	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of Cp4.1LG05g01970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023534693.1	2.10e-123	100.00	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
XP_022995887.1	4.96e-122	98.90	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_022958457.1	2.26e-118	96.13	putative invertase inhibitor [Cucurbita moschata]	[more]
KAG6606222.1	6.98e-113	98.24	hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023523101.1	7.93e-100	100.00	putative invertase inhibitor, partial [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cp4.1LG05g01970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K084	2.40e-122	98.90	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111491280 PE=4 SV...	[more]
A0A6J1H1W1	1.10e-118	96.13	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 ...	[more]
A0A6J1ICI2	2.46e-79	73.99	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV...	[more]
A0A0A0LHE9	6.05e-77	67.96	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 S...	[more]
A0A5A7TAE5	1.42e-75	67.40	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Cp4.1LG05g01970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46970.1	1.1e-21	39.61	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	1.4e-21	35.26	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	4.2e-21	36.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	2.5e-18	35.43	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	7.4e-18	34.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..176 e-value: 4.3E-26 score: 102.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..178 e-value: 6.9E-33 score: 111.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..175 e-value: 2.0E-26 score: 93.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 25..179 e-value: 2.3E-33 score: 117.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 25..178
None	No IPR available	PANTHER	PTHR31080:SF239	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 16..179
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 16..179
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 32..179 e-value: 9.39198E-41 score: 132.874

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g01970.1	Cp4.1LG05g01970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity