Cp4.1LG05g01330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g01330
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptioniron-sulfur protein NUBPL
LocationCp4.1LG05: 244957 .. 249486 (-)
RNA-Seq ExpressionCp4.1LG05g01330
SyntenyCp4.1LG05g01330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGCCCAAATGTACGATTGCTCCTTGTCTCGTAGGTCCTTCACTGTTGCCGTCGTCTCCTATAATTTGTAGTGAAGAATTGGAGCTTAGAATCGAGACTGTGTAGGCAGAAGTTCTTCTTTATTGGCGTTTTCTACTAGTAAGAAATTCATGAAGCGGTTGTCATGGTTCCTCTCCTGTAAACGTGTGAGTTTTTCTCTCAGCTTCCTTTAATGCTTCGCGGTTCTCTTGACATGTCCTTCATTTACCTCCTTAATCAAAATGGAGGCTGAATTCAGTGATTGCCTTTCTGTATGTTCATGTAGAGGGTTGGAGGTGTGAGAGGATATTCCGCCACCAAAAAGGAGCTGAAAATTGATGGAATTAAGCATACTATAGCCATTGCTTCTGGCAAAGGAGGTGTGGGCAAGTCCACCACTGCCGGTACTTTCTTTTGCTCTGTTGCCTGTTTCTTAATTTTAGGTTTTTGGTACGGTGACCATTTTTCTAATTGTAATCTTAGGTAATCAAGTGGGCTAATCAAGCTGATTCCCTTGCATAATCTTCATTTTTCTCCATTCTTGTCTACCTCTAATGTTTAATTGCGTCCTTTTGAGTTTAGGACCAAAATGAAACGCAATTTAGACTCCAGGAGTAATCCAGAATGCATCATTTTTAAAGTTGATCGAAGGTGGTTTTAAAAAAGGGATTTTTTTTTTTTTTTTTTTGGACGTTTCTGATTATCTTCTTTATTGCTTTATGTGAATAATCGTCACTTAGATTTCTAGTCTGTTATTGAGGCACTAATAAATCCTTCGTACTTGTTACTACGTGGTGGTTGTTCTTATACAATTCGGTGGGTGGCAGTAAATTTGGCCGTTGCACTAGCAAACAAGTGTCACCTGAATGTCGGATTACTTGATGCTGATGTATATGGACCCAATGTTCCAATTATGATGAAAATCCATCAAAAGCCAGAGTTGACTGAAGGTACTTTATAACCTTTTGTAATTACTAATATAGCATAGCTCAACTAGCATAAAACAGTGTCCTTGACCAAGGGGTCTCGTTGCAGGGATATGGATATGCTCGTATTTTCTGTCTTAAGCCTGCCTGTTGCTTGAACTACCCTTATTTAAATCTAAACTAGCTTTTAGCCTTCACCGGTGTAATAGATTTCTGAACCGGCCATCATTACTCTCACTGATAATTACATTTGTTTATTGTGGTCGACTTGATTTGGTTCAAGTATTTTTTCCATTTTAGATATCTGAATTTAAGCGCTACTGCAATAATCCTATCAATCTCCAAGAAATTCTTGTTGGTCCAATATTATAAGTGCCTTCTCGAGTGTACTTTTTTTTTATTTTCCTGCATGATAAGTTAAGTCAGTTCCCAAGAATCGGTGGATTATCTTACTTTCTTTAATTTTCCGTGCCTGAAAAATCTGTTTACCTTCGGAAGAATTATAGCCAGCCAGGCTAATCACATTTGCTTTCTAGGCTTTGATATGATTCCCAGTGAGCATCCTGATGTCCATGTTTTAAGTTTTTTCAAAGATACAATTGCCCCATAATGTATCACTTTATCGTTCTTTCTTTCACAGATAGAAAAATGGTTCCTGTTGAGAGCTATGGAGTTAAATGCATGTCAATGGGATTACTCGTGGAAAACAATGCTGCTCTTGTGTGGAGGGGTCCTATGGTTTGTTTTCCCGAAACTTTCTGTTGAGTATTCCTCCAATATTTCGAGATAACATGGCTTTAATGCTTCTTATCTTCCTGAATGATAAGTTTCTTCATTTCTCTACCACCAAGGTGGCAATAGATCTCCTATGACCAAGATGTATAATTCATCTTTTTTTTGTAAATATTAAGTTGATCTCCTTACCATCTTGAAAGGAGATTGAGTAAATGAAATTATAAGGAAAAGGGCTCACCTGGTGGATTGGAACATAATTACTTTGCGCACAGATCAACGGGGCAGTACCATGGGCAACTGCTGTAAAGGAATGTGGCTATTCTAGAGGAAATGCTTTGGTAGATCCCCTTGTGAACTTGAAGTATTTTGTAGTTGGTTGCTAGCAAATTAAGTAGAGATAGGAGTAGAGCCCTGGTTTGACGTGGCTAGTATTTGAAATTGTGTTATCGGCTTCACCTCACTTTAGATTGGTTCACGTTCAAGGGAAATAATTTTTTTGGAAGGTGGGATTAATCATGATTTAGGTTGTTGGGCCTTTCCCGCTTGCTAATGCCCACATATTAAAAACTATATTTTTCTTGCTTTCCTTTGAGAAGATTCATACTAATGATAATATTAAAAGGATGCATCCAAATATATCTCTTTGGAATATTAAAAATATGTTTTTTTTTATACAAGATTTAGAGGATATCGACCATATTATTGTGCACCAAGACTCAGGGTAATCATTCGAGTAGGGTGACATTACCTCTTGCTCTAGCGCTTGTGGTACTTGGTTCATTGTAGGCCACATAGCCAGGTTGCCTTTGGTGCACCTTGCTGCTATTTCAAGGATTGATTGTATGGGTTCGCTATCTGCAGTCTTGTACCTTTTATCCACCATATGCTCCATCTGGTTTTCTTCCTAGAATGCTCATCCAACCAACGACATTCAAACCCCTACTTGAGAAAAATGAAACTGTAGGCCTCTTCCCTGTTATTATCTCCAACAAGAGAACTCCAAAGCTATAAACGTTTGAATGTTTGACTTCTTTGGTGGAGCTTTATGATTTTACCTTCTTTTTTACTGTTTGAAGAGCCTGAAACCTCAATGTAATTCGGTTCCTCACTGATCCGACCATGTATAATTTTTTATAACTAAAGACTTGATTAATATTTTTTGCAAGAAACACTCTAGATATCATGTTGTCAATCAGCCTTTGACTCAACTTCAATGATTGCTTCTTCTTTTTTTCTTTTTTTTTCCCTCCTAATCTAACTTGAATTTGACTTGTACAACTAAACATGTACTTCAAAAGACTATGCTTGAAGAATAATTTGCTTTCATCATTGAACTCTTTCCACATTTCTTTAGAACTTGGCTAATTTCGCAGGTGTCAAGTGCTCTGGAGAAAATGACAAGGGGAGTTGCTTGGGGAGATCTTGATATTCTTGTTGTTGATATGCCTCCTGGTACAGGCGATGTTCAGATTACAATGTCACAGAGATTGTTACTCTCTGGTAGGTAAAAATTTGTGATTTCACCCTTTGTTCTTTGGCAGGTTTGTTAATGGAATTGTTGTGTTTCTAATCCAAATGCTATCGGTACTGAGTTATTTGGAAGTATGATGGTAGAAATATAGCAGTTTCTCATATATAATTCTGTTCACAAATTTTATTTATTTGTAATAACACCTATAATTCATTTTCCTTGCTGTTAGGTGCATTGATTGTTTCAACGCCTCAGGATGTTGCATTAATGGATGCTCGTAGGGGAATTAAAATGTTTTCTAATGTCCATGTTCCGGTGTGAATTTCTCTATATTATTTTGCTAAATTTCCAAACCACCCTGATTGCAGTCTCGTTCATTATCAACATCCTAAAAAGTATAAAAATGTTACAATAATGTGGTTAAAATGTTTCTTGATAGTCTTTAGTCTTTACCTTATTATTCCATGCATTCATTGGATGATAAAGAATCTGAATATCAATTCTCTTCATTTTCACACTCAGACATTGCTAAGTTTTCTGAAAGAATCTGAATGTTATTGACTACTGCTTCAGATTCTGGGAATTGTAGAGAACATGAGCTACTTTACATGTCCACATTGTGATGAACGCTCCTATATCTTTGGGAGGGGAGGAAGTCGCAAGGCAGCTGATGAGATGGGTATCCCATTCGTGGGTGAGGTACTTGCTAATACGAGCCATTTAATTAGTATGCATGACCAATTATGTTGATTTCCAGATGATAATGTCATATGAATACGCCGATCAATGAAGAATTTCTATAGGTGGGTCATTTTCCATACTAGCCAACTTGTGGATAACTTGTCACAGGTACCATTAGAAGAGAAGAACAGGAAAGGTTGTGACGAAGGTGTCCCTATTGTAATGTCAGAACCAAATTCCCTGGTTTCTACAAAATATGTGGAAATTGCTGAGAAAGTGATGGAGCGAATTGAGGAAGTGGAGAGGCAAAAACTACTCTATCCAAAGATTCAATTATAACCACAGCAGCCCCGAGATTTCTTTTTCTTTTTAATATATAACAGGACAATAGATTATGTTTCATTCCTATCATTCCAGCTTCATTCCTTGCAAGATAGAAAAAAAAATATTGAATTATGCTCATTTTGGCTCCTTTTGCATCGTTCTTAATGAATGAGATATTTATTTATTTTAACTCTTATTAGTCCCTCCAAAAGATTCTATGATGACTTTGTTATTCAACACATACAATTCTATGATGACTTTGTTATTCAACACATACAATTCTATGATGACTTTGTTATTCAACACATACAATTCGATAGACAACCTATACATTTTTGGTCCAAATGCTATAGTAGAAGTGCAATTGCTTATTA

mRNA sequence

AGAGCCCAAATGTACGATTGCTCCTTGTCTCGTAGGTCCTTCACTGTTGCCGTCGTCTCCTATAATTTGTAGTGAAGAATTGGAGCTTAGAATCGAGACTGTGTAGGCAGAAGTTCTTCTTTATTGGCGTTTTCTACTAGTAAGAAATTCATGAAGCGGTTGTCATGGTTCCTCTCCTGTAAACGTAGGGTTGGAGGTGTGAGAGGATATTCCGCCACCAAAAAGGAGCTGAAAATTGATGGAATTAAGCATACTATAGCCATTGCTTCTGGCAAAGGAGGTGTGGGCAAGTCCACCACTGCCGTAAATTTGGCCGTTGCACTAGCAAACAAGTGTCACCTGAATGTCGGATTACTTGATGCTGATGTATATGGACCCAATGTTCCAATTATGATGAAAATCCATCAAAAGCCAGAGTTGACTGAAGATAGAAAAATGGTTCCTGTTGAGAGCTATGGAGTTAAATGCATGTCAATGGGATTACTCGTGGAAAACAATGCTGCTCTTGTGTGGAGGGGTCCTATGGTGTCAAGTGCTCTGGAGAAAATGACAAGGGGAGTTGCTTGGGGAGATCTTGATATTCTTGTTGTTGATATGCCTCCTGGTACAGGCGATGTTCAGATTACAATGTCACAGAGATTGTTACTCTCTGGTGCATTGATTGTTTCAACGCCTCAGGATGTTGCATTAATGGATGCTCGTAGGGGAATTAAAATGTTTTCTAATGTCCATGTTCCGATTCTGGGAATTGTAGAGAACATGAGCTACTTTACATGTCCACATTGTGATGAACGCTCCTATATCTTTGGGAGGGGAGGAAGTCGCAAGGCAGCTGATGAGATGGGTATCCCATTCGTGGGTGAGGTACCATTAGAAGAGAAGAACAGGAAAGGTTGTGACGAAGGTGTCCCTATTGTAATGTCAGAACCAAATTCCCTGGTTTCTACAAAATATGTGGAAATTGCTGAGAAAGTGATGGAGCGAATTGAGGAAGTGGAGAGGCAAAAACTACTCTATCCAAAGATTCAATTATAACCACAGCAGCCCCGAGATTTCTTTTTCTTTTTAATATATAACAGGACAATAGATTATGTTTCATTCCTATCATTCCAGCTTCATTCCTTGCAAGATAGAAAAAAAAATATTGAATTATGCTCATTTTGGCTCCTTTTGCATCGTTCTTAATGAATGAGATATTTATTTATTTTAACTCTTATTAGTCCCTCCAAAAGATTCTATGATGACTTTGTTATTCAACACATACAATTCTATGATGACTTTGTTATTCAACACATACAATTCTATGATGACTTTGTTATTCAACACATACAATTCGATAGACAACCTATACATTTTTGGTCCAAATGCTATAGTAGAAGTGCAATTGCTTATTA

Coding sequence (CDS)

ATGAAGCGGTTGTCATGGTTCCTCTCCTGTAAACGTAGGGTTGGAGGTGTGAGAGGATATTCCGCCACCAAAAAGGAGCTGAAAATTGATGGAATTAAGCATACTATAGCCATTGCTTCTGGCAAAGGAGGTGTGGGCAAGTCCACCACTGCCGTAAATTTGGCCGTTGCACTAGCAAACAAGTGTCACCTGAATGTCGGATTACTTGATGCTGATGTATATGGACCCAATGTTCCAATTATGATGAAAATCCATCAAAAGCCAGAGTTGACTGAAGATAGAAAAATGGTTCCTGTTGAGAGCTATGGAGTTAAATGCATGTCAATGGGATTACTCGTGGAAAACAATGCTGCTCTTGTGTGGAGGGGTCCTATGGTGTCAAGTGCTCTGGAGAAAATGACAAGGGGAGTTGCTTGGGGAGATCTTGATATTCTTGTTGTTGATATGCCTCCTGGTACAGGCGATGTTCAGATTACAATGTCACAGAGATTGTTACTCTCTGGTGCATTGATTGTTTCAACGCCTCAGGATGTTGCATTAATGGATGCTCGTAGGGGAATTAAAATGTTTTCTAATGTCCATGTTCCGATTCTGGGAATTGTAGAGAACATGAGCTACTTTACATGTCCACATTGTGATGAACGCTCCTATATCTTTGGGAGGGGAGGAAGTCGCAAGGCAGCTGATGAGATGGGTATCCCATTCGTGGGTGAGGTACCATTAGAAGAGAAGAACAGGAAAGGTTGTGACGAAGGTGTCCCTATTGTAATGTCAGAACCAAATTCCCTGGTTTCTACAAAATATGTGGAAATTGCTGAGAAAGTGATGGAGCGAATTGAGGAAGTGGAGAGGCAAAAACTACTCTATCCAAAGATTCAATTATAA

Protein sequence

MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL
Homology
BLAST of Cp4.1LG05g01330 vs. ExPASy Swiss-Prot
Match: Q54F15 (Iron-sulfur protein NUBPL OS=Dictyostelium discoideum OX=44689 GN=nubpl PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 7.7e-78
Identity = 141/285 (49.47%), Postives = 210/285 (73.68%), Query Frame = 0

Query: 5   SWFLSCKRRVGGVRGYSATK-KELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALANKCH 64
           ++F + K ++ G  G+   +  ++ I+GIK+ IA++S KGGVGKST AVN+A+ L++  +
Sbjct: 28  NYFSNNKIQLHGGSGHRQPQVTKVAIEGIKNIIAVSSAKGGVGKSTCAVNIALGLSSH-N 87

Query: 65  LNVGLLDADVYGPNVPIMM--KIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALVW 124
           L+VGLLD DV+GP++P+MM  K H+KP   E  +M+P+++YG+KCMSMG LV  +  ++W
Sbjct: 88  LSVGLLDVDVFGPSIPLMMDLKNHEKPFTNELNQMIPLQNYGIKCMSMGFLVNEDDPIIW 147

Query: 125 RGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVALM 184
           RGPMV SALEK+ R   WG LD+LV D+PPGTGD  +TM QR+ L+GA+IVSTPQDVAL 
Sbjct: 148 RGPMVGSALEKLLRQTDWGHLDVLVCDLPPGTGDAILTMCQRVPLTGAVIVSTPQDVALA 207

Query: 185 DARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVPL 244
           D  RG+ MF  V VPILG+VENMSYF CPHC+E ++IFG  G++  A +MGI F+G+VP+
Sbjct: 208 DVVRGVNMFKKVEVPILGLVENMSYFNCPHCNESTHIFGNEGAKNTAKKMGINFLGDVPI 267

Query: 245 EEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQK 287
             + R+  D G PI +++P+S  +  Y +I++++++++E +   +
Sbjct: 268 HLQIRETSDSGKPITVTQPDSPQAKNYKDISKEIIKQLEIINNDE 311

BLAST of Cp4.1LG05g01330 vs. ExPASy Swiss-Prot
Match: Q8TB37 (Iron-sulfur protein NUBPL OS=Homo sapiens OX=9606 GN=NUBPL PE=1 SV=3)

HSP 1 Score: 290.0 bits (741), Expect = 2.9e-77
Identity = 141/256 (55.08%), Postives = 187/256 (73.05%), Query Frame = 0

Query: 25  KELKIDGIKHTIAIASGKGGVGKSTTAVNLAVAL-ANKCHLNVGLLDADVYGPNVPIMMK 84
           K+  I+G+K  I +ASGKGGVGKSTTAVNLA+AL AN     +GLLD DVYGP+VP MM 
Sbjct: 59  KQKPIEGVKQVIVVASGKGGVGKSTTAVNLALALAANDSSKAIGLLDVDVYGPSVPKMMN 118

Query: 85  IHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLD 144
           +   PEL++   M P+ +YG+ CMSMG LVE +  +VWRG MV SA+EK+ R V WG LD
Sbjct: 119 LKGNPELSQSNLMRPLLNYGIACMSMGFLVEESEPVVWRGLMVMSAIEKLLRQVDWGQLD 178

Query: 145 ILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVEN 204
            LVVDMPPGTGDVQ+++SQ + ++GA+IVSTPQD+ALMDA +G +MF  VHVP+LG+V+N
Sbjct: 179 YLVVDMPPGTGDVQLSVSQNIPITGAVIVSTPQDIALMDAHKGAEMFRRVHVPVLGLVQN 238

Query: 205 MSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSL 264
           MS F CP C  +++IFG  G+RK A  +G+  +G++PL    R+  D G PIV S+P S 
Sbjct: 239 MSVFQCPKCKHKTHIFGADGARKLAQTLGLEVLGDIPLHLNIREASDTGQPIVFSQPESD 298

Query: 265 VSTKYVEIAEKVMERI 280
            +  Y+ IA +V+ R+
Sbjct: 299 EAKAYLRIAVEVVRRL 314

BLAST of Cp4.1LG05g01330 vs. ExPASy Swiss-Prot
Match: Q9CWD8 (Iron-sulfur protein NUBPL OS=Mus musculus OX=10090 GN=Nubpl PE=1 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 1.9e-76
Identity = 141/257 (54.86%), Postives = 187/257 (72.76%), Query Frame = 0

Query: 25  KELKIDGIKHTIAIASGKGGVGKSTTAVNLAVAL-ANKCHLNVGLLDADVYGPNVPIMMK 84
           K+  I+G++  I +ASGKGGVGKSTTAVNLA+AL AN     VGLLD DVYGP++P MM 
Sbjct: 59  KQKPIEGVREVIVVASGKGGVGKSTTAVNLALALAANDSSKAVGLLDVDVYGPSIPKMMN 118

Query: 85  IHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLD 144
           +   PEL+ +  M P+ +YG+ CMSMG LVE  A LVWRG MV SA+EK+ R V WG LD
Sbjct: 119 LRGNPELSPNNLMRPLLNYGIACMSMGFLVEETAPLVWRGLMVMSAIEKLLRQVDWGQLD 178

Query: 145 ILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVEN 204
            LVVDMPPGTGDVQ+++SQ + +SGA+IVSTPQD+ALMDA +G +MF  V+VP+LG+V+N
Sbjct: 179 YLVVDMPPGTGDVQLSVSQNIPISGAVIVSTPQDIALMDAHKGAEMFRKVNVPVLGLVQN 238

Query: 205 MSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSL 264
           MS F CP C  +++IFG  G+RK A  + +  +G+VPL    R+  D G P+V S+P S 
Sbjct: 239 MSVFQCPKCKHKTHIFGADGARKLAQTLDLDVLGDVPLHLSIREASDMGQPVVFSQPGSD 298

Query: 265 VSTKYVEIAEKVMERIE 281
            +  Y+ IA +V+ R++
Sbjct: 299 EAKAYLHIASEVVRRLK 315

BLAST of Cp4.1LG05g01330 vs. ExPASy Swiss-Prot
Match: O24999 (Iron-sulfur cluster carrier protein OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=mrp PE=3 SV=2)

HSP 1 Score: 255.8 bits (652), Expect = 6.1e-67
Identity = 125/258 (48.45%), Postives = 179/258 (69.38%), Query Frame = 0

Query: 32  IKHTIAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIHQKPELT 91
           IKH + I+SGKGGVGKSTT+VNL++ALAN  +  VGLLDADVYGPN+P MM +     + 
Sbjct: 96  IKHVVMISSGKGGVGKSTTSVNLSIALAN-LNQKVGLLDADVYGPNIPRMMGLQSADVIM 155

Query: 92  E--DRKMVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLDILVVDM 151
           +   +K++P++++GV  MSMGLL +   +L+WRGPM+  A+E+M   + WGDLD+LVVDM
Sbjct: 156 DPSGKKLIPLKAFGVSVMSMGLLYDEGQSLIWRGPMLMRAIEQMLSDIIWGDLDVLVVDM 215

Query: 152 PPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVENMSYFTC 211
           PPGTGD Q+T++Q + LS  + V+TPQ V+L DA+R + MF  +H+PI GIVENM  F C
Sbjct: 216 PPGTGDAQLTLAQAVPLSAGITVTTPQIVSLDDAKRSLDMFKKLHIPIAGIVENMGSFVC 275

Query: 212 PHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSLVSTKYV 271
            HC + S IFG     +  +      + ++PLE K R G D G PIV+S PNS+ +  + 
Sbjct: 276 EHCKKESEIFGSNSMSELLEAYHTQILAKLPLEPKVRLGGDRGEPIVISHPNSVSAKIFE 335

Query: 272 EIAEKVMERIEEVERQKL 288
           ++A+ +   +E V+++KL
Sbjct: 336 KMAQDLSAFLERVKKEKL 352

BLAST of Cp4.1LG05g01330 vs. ExPASy Swiss-Prot
Match: Q9ZMM5 (Iron-sulfur cluster carrier protein OS=Helicobacter pylori (strain J99 / ATCC 700824) OX=85963 GN=mrp PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.0e-66
Identity = 125/258 (48.45%), Postives = 179/258 (69.38%), Query Frame = 0

Query: 32  IKHTIAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIHQKPELT 91
           IKH + I+SGKGGVGKSTT+VNL++ALAN  +  VGLLDADVYGPN+P MM +     + 
Sbjct: 96  IKHVVMISSGKGGVGKSTTSVNLSIALAN-LNQKVGLLDADVYGPNIPRMMGLQNADVIM 155

Query: 92  E--DRKMVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLDILVVDM 151
           +   +K++P++++GV  MSMGLL +   +L+WRGPM+  A+E+M   + WGDLD+LVVDM
Sbjct: 156 DPSGKKLIPLKAFGVSVMSMGLLYDEGQSLIWRGPMLMRAIEQMLSDIIWGDLDVLVVDM 215

Query: 152 PPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVENMSYFTC 211
           PPGTGD Q+T++Q + LS  + V+TPQ V+L DA+R + MF  +H+PI GIVENM  F C
Sbjct: 216 PPGTGDAQLTLAQAVPLSAGITVTTPQIVSLDDAKRSLDMFKKLHIPIAGIVENMGSFVC 275

Query: 212 PHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSLVSTKYV 271
            HC + S IFG        +      + ++PLE K R G D+G PIV+S P S+ +  + 
Sbjct: 276 EHCKKESEIFGSNSMSGLLEAYNTQILAKLPLEPKVRLGGDKGEPIVISHPTSVSAKIFE 335

Query: 272 EIAEKVMERIEEVERQKL 288
           ++A+ +   +++VER+KL
Sbjct: 336 KMAKDLSAFLDKVEREKL 352

BLAST of Cp4.1LG05g01330 vs. NCBI nr
Match: XP_023534279.1 (iron-sulfur protein NUBPL isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588 bits (1516), Expect = 7.23e-212
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV
Sbjct: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL
Sbjct: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294

BLAST of Cp4.1LG05g01330 vs. NCBI nr
Match: KAG6606394.1 (Iron-sulfur protein NUBPL, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585 bits (1508), Expect = 1.20e-210
Identity = 292/294 (99.32%), Postives = 293/294 (99.66%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWF SCKRRVGGVRGYSA+KKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWFFSCKRRVGGVRGYSASKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV
Sbjct: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL
Sbjct: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294

BLAST of Cp4.1LG05g01330 vs. NCBI nr
Match: XP_022931224.1 (iron-sulfur protein NUBPL isoform X1 [Cucurbita moschata])

HSP 1 Score: 583 bits (1503), Expect = 6.93e-210
Identity = 291/294 (98.98%), Postives = 293/294 (99.66%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWF SCKRRVGGVRGYSA+KKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWFFSCKRRVGGVRGYSASKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV
Sbjct: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLL+PKIQL
Sbjct: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLHPKIQL 294

BLAST of Cp4.1LG05g01330 vs. NCBI nr
Match: XP_022995177.1 (iron-sulfur protein NUBPL isoform X1 [Cucurbita maxima])

HSP 1 Score: 574 bits (1479), Expect = 3.04e-206
Identity = 288/294 (97.96%), Postives = 290/294 (98.64%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWF SCKRRVGGVRGYSA+KKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWFFSCKRRVGGVRGYSASKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KC LNVGLLDADVYGPNVPIMMKIHQKPEL EDRKMVP+ESYGVKCMSMGLLVENNAALV
Sbjct: 61  KCRLNVGLLDADVYGPNVPIMMKIHQKPELNEDRKMVPIESYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEE ERQKLLYPKIQL
Sbjct: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEE-ERQKLLYPKIQL 293

BLAST of Cp4.1LG05g01330 vs. NCBI nr
Match: XP_038888219.1 (iron-sulfur protein NUBPL isoform X1 [Benincasa hispida])

HSP 1 Score: 533 bits (1372), Expect = 2.08e-189
Identity = 265/294 (90.14%), Postives = 280/294 (95.24%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWF S + R+GGVRGYSA+KKEL+I GIKH IAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 33  MKRLSWFFSYECRLGGVRGYSASKKELQIHGIKHAIAIASGKGGVGKSTTAVNLAVALAN 92

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KC L VGLLDADVYGPNVPIMMKIHQKPE+TEDRKMVPVE+YGVKCMSMGLLVENNAALV
Sbjct: 93  KCQLKVGLLDADVYGPNVPIMMKIHQKPEVTEDRKMVPVENYGVKCMSMGLLVENNAALV 152

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGV+WGDLDILVVDMPPGTGDVQITMSQRL LSG L+VSTPQDVAL
Sbjct: 153 WRGPMVSSALEKMTRGVSWGDLDILVVDMPPGTGDVQITMSQRLSLSGVLVVSTPQDVAL 212

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHC ERSYIFG GGSRKAADEMGI F+GEVP
Sbjct: 213 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCGERSYIFGSGGSRKAADEMGITFLGEVP 272

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRK CDEG+PIV+SEPNSLVST YVEIA+KVM++IEEVERQKLL+PKIQL
Sbjct: 273 LEEKNRKCCDEGIPIVISEPNSLVSTAYVEIAQKVMKQIEEVERQKLLHPKIQL 326

BLAST of Cp4.1LG05g01330 vs. ExPASy TrEMBL
Match: A0A6J1ETQ4 (iron-sulfur protein NUBPL isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437465 PE=3 SV=1)

HSP 1 Score: 583 bits (1503), Expect = 3.36e-210
Identity = 291/294 (98.98%), Postives = 293/294 (99.66%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWF SCKRRVGGVRGYSA+KKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWFFSCKRRVGGVRGYSASKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV
Sbjct: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLL+PKIQL
Sbjct: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLHPKIQL 294

BLAST of Cp4.1LG05g01330 vs. ExPASy TrEMBL
Match: A0A6J1JY28 (iron-sulfur protein NUBPL isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490799 PE=3 SV=1)

HSP 1 Score: 574 bits (1479), Expect = 1.47e-206
Identity = 288/294 (97.96%), Postives = 290/294 (98.64%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSWF SCKRRVGGVRGYSA+KKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWFFSCKRRVGGVRGYSASKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KC LNVGLLDADVYGPNVPIMMKIHQKPEL EDRKMVP+ESYGVKCMSMGLLVENNAALV
Sbjct: 61  KCRLNVGLLDADVYGPNVPIMMKIHQKPELNEDRKMVPIESYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEE ERQKLLYPKIQL
Sbjct: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEE-ERQKLLYPKIQL 293

BLAST of Cp4.1LG05g01330 vs. ExPASy TrEMBL
Match: A0A5A7UNF7 (Iron-sulfur protein NUBPL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00620 PE=3 SV=1)

HSP 1 Score: 525 bits (1352), Expect = 3.48e-187
Identity = 261/294 (88.78%), Postives = 278/294 (94.56%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSW  S +R++G VRGYSA++KEL+I GIKH IAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWLFSGERKLGSVRGYSASRKELQIHGIKHAIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KC L VGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVE+YGVKCMSMGLLVENNAALV
Sbjct: 61  KCQLKVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVENYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPM+SSAL+KMTRGV+WGDLDILVVDMPPGTGDVQITMSQRL LSGALIVSTPQDVAL
Sbjct: 121 WRGPMISSALDKMTRGVSWGDLDILVVDMPPGTGDVQITMSQRLSLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHC ERSYIFG GGSRKAADEM I F+GEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCGERSYIFGNGGSRKAADEMCITFLGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRK CDEG+PIV+SEPNSLVS  YVEIA+KVME+IEEV+RQKLL+PKIQL
Sbjct: 241 LEEKNRKCCDEGIPIVISEPNSLVSIAYVEIAQKVMEQIEEVDRQKLLHPKIQL 294

BLAST of Cp4.1LG05g01330 vs. ExPASy TrEMBL
Match: A0A1S3BLW5 (iron-sulfur protein NUBPL OS=Cucumis melo OX=3656 GN=LOC103491273 PE=3 SV=1)

HSP 1 Score: 525 bits (1352), Expect = 3.48e-187
Identity = 261/294 (88.78%), Postives = 278/294 (94.56%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKRLSW  S +R++G VRGYSA++KEL+I GIKH IAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRLSWLFSGERKLGSVRGYSASRKELQIHGIKHAIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           KC L VGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVE+YGVKCMSMGLLVENNAALV
Sbjct: 61  KCQLKVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVENYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPM+SSAL+KMTRGV+WGDLDILVVDMPPGTGDVQITMSQRL LSGALIVSTPQDVAL
Sbjct: 121 WRGPMISSALDKMTRGVSWGDLDILVVDMPPGTGDVQITMSQRLSLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHC ERSYIFG GGSRKAADEM I F+GEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCGERSYIFGNGGSRKAADEMCITFLGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRK CDEG+PIV+SEPNSLVS  YVEIA+KVME+IEEV+RQKLL+PKIQL
Sbjct: 241 LEEKNRKCCDEGIPIVISEPNSLVSIAYVEIAQKVMEQIEEVDRQKLLHPKIQL 294

BLAST of Cp4.1LG05g01330 vs. ExPASy TrEMBL
Match: A0A6J1CW38 (iron-sulfur protein NUBPL OS=Momordica charantia OX=3673 GN=LOC111015332 PE=3 SV=1)

HSP 1 Score: 525 bits (1351), Expect = 4.94e-187
Identity = 263/294 (89.46%), Postives = 277/294 (94.22%), Query Frame = 0

Query: 1   MKRLSWFLSCKRRVGGVRGYSATKKELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALAN 60
           MKR SWF S K R+GGVRGYSATKKEL+IDGIKH IAIASGKGGVGKSTTAVNLAVALAN
Sbjct: 1   MKRFSWFFSAKCRLGGVRGYSATKKELQIDGIKHAIAIASGKGGVGKSTTAVNLAVALAN 60

Query: 61  KCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRKMVPVESYGVKCMSMGLLVENNAALV 120
           K  L VGLLDADVYGPNVP MM+I QKPELTEDRKM+PVE+YGVKCMSMGLLVENNAALV
Sbjct: 61  KWQLKVGLLDADVYGPNVPTMMQIQQKPELTEDRKMIPVENYGVKCMSMGLLVENNAALV 120

Query: 121 WRGPMVSSALEKMTRGVAWGDLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVAL 180
           WRGPMVSSALEKMTRGVAWG+LDIL+VDMPPGTGDVQIT+SQRL LSGALIVSTPQDVAL
Sbjct: 121 WRGPMVSSALEKMTRGVAWGNLDILLVDMPPGTGDVQITISQRLSLSGALIVSTPQDVAL 180

Query: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVP 240
           MDARRGIKMFSNVHVPILGIVENMSYFTCPHC ERSYIFG GGSRKAADEMGI FVGEVP
Sbjct: 181 MDARRGIKMFSNVHVPILGIVENMSYFTCPHCGERSYIFGNGGSRKAADEMGIIFVGEVP 240

Query: 241 LEEKNRKGCDEGVPIVMSEPNSLVSTKYVEIAEKVMERIEEVERQKLLYPKIQL 294
           LEEKNRK CDEGVPIV+SEPNS+VST YV+IA+KVM+RIEEVE QKLL+PKIQL
Sbjct: 241 LEEKNRKCCDEGVPIVISEPNSVVSTAYVDIAQKVMQRIEEVESQKLLHPKIQL 294

BLAST of Cp4.1LG05g01330 vs. TAIR 10
Match: AT4G19540.1 (IND1(iron-sulfur protein required for NADH dehydrogenase)-like )

HSP 1 Score: 366.7 bits (940), Expect = 1.7e-101
Identity = 175/257 (68.09%), Postives = 215/257 (83.66%), Query Frame = 0

Query: 26  ELKIDGIKHTIAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIH 85
           EL++ G+K  IA+ASGKGGVGKS+TAVNLAVALANKC L +GLLDADVYGP+VPIMM I+
Sbjct: 36  ELRLHGVKDIIAVASGKGGVGKSSTAVNLAVALANKCELKIGLLDADVYGPSVPIMMNIN 95

Query: 86  QKPELTEDRKMVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLDIL 145
           QKP++ +D KM+PVE+YGVKCMSMGLLVE +A LVWRGPMV SAL KMT+GV WGDLDIL
Sbjct: 96  QKPQVNQDMKMIPVENYGVKCMSMGLLVEKDAPLVWRGPMVMSALAKMTKGVDWGDLDIL 155

Query: 146 VVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVENMS 205
           VVDMPPGTGD QI++SQ L LSGA+IVSTPQDVAL DA RGI MF  V VPILG+VENMS
Sbjct: 156 VVDMPPGTGDAQISISQNLKLSGAVIVSTPQDVALADANRGISMFDKVRVPILGLVENMS 215

Query: 206 YFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSLVS 265
            F CPHC+E S+IFG+ G+R+ A + G+  +GE+PLE   R+G DEGVP+V+S P S+VS
Sbjct: 216 CFVCPHCNEPSFIFGKEGARRTAAKKGLKLIGEIPLEMSIREGSDEGVPVVVSSPGSIVS 275

Query: 266 TKYVEIAEKVMERIEEV 283
             Y ++A+ V++ ++E+
Sbjct: 276 KAYQDLAQNVVKGLKEL 292

BLAST of Cp4.1LG05g01330 vs. TAIR 10
Match: AT3G24430.1 (ATP binding )

HSP 1 Score: 189.1 bits (479), Expect = 5.0e-48
Identity = 102/256 (39.84%), Postives = 158/256 (61.72%), Query Frame = 0

Query: 32  IKHTIAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIHQK-PEL 91
           I + IA++S KGGVGKST AVNLA  LA      VG+ DADVYGP++P M+    +  E+
Sbjct: 175 ISNIIAVSSCKGGVGKSTVAVNLAYTLAG-MGARVGIFDADVYGPSLPTMVNPESRILEM 234

Query: 92  TEDRK-MVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRGVAWGDLDILVVDM 151
             ++K ++P E  GVK +S G   +  A  + RGPMVS  + ++     WG+LD LV+DM
Sbjct: 235 NPEKKTIIPTEYMGVKLVSFGFAGQGRA--IMRGPMVSGVINQLLTTTEWGELDYLVIDM 294

Query: 152 PPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGIVENMSYFTC 211
           PPGTGD+Q+T+ Q   L+ A+IV+TPQ +A +D  +G++MFS + VP + +VENM +F  
Sbjct: 295 PPGTGDIQLTLCQVAPLTAAVIVTTPQKLAFIDVAKGVRMFSKLKVPCVAVVENMCHFDA 354

Query: 212 PHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEPNSLVSTKYV 271
               +R Y FG+G   +   + GIP + ++P+        D G P V+S+P S V+  + 
Sbjct: 355 D--GKRYYPFGKGSGSEVVKQFGIPHLFDLPIRPTLSASGDSGTPEVVSDPLSDVARTFQ 414

Query: 272 EIAEKVMERIEEVERQ 286
           ++   V+++  ++ +Q
Sbjct: 415 DLGVCVVQQCAKIRQQ 425

BLAST of Cp4.1LG05g01330 vs. TAIR 10
Match: AT5G50960.1 (nucleotide binding protein 35 )

HSP 1 Score: 155.6 bits (392), Expect = 6.1e-38
Identity = 94/269 (34.94%), Postives = 148/269 (55.02%), Query Frame = 0

Query: 28  KIDGIKHTIAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIHQK 87
           ++  +KH I + SGKGGVGKST +  L+ ALA   H  VGL+D D+ GP++P M+ +  +
Sbjct: 54  RMSTVKHKILVLSGKGGVGKSTFSAQLSFALAGMDH-QVGLMDIDICGPSIPKMLGLEGQ 113

Query: 88  PELTEDRKMVPV---ESYGVKCMSMGLLVEN-NAALVWRGPMVSSALEKMTRGVAWGDLD 147
                +    PV   ++ GV  MS+G ++ N + A++WRGP  +  +++  + V WG++D
Sbjct: 114 EIHQSNLGWSPVYVEDNLGV--MSIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEID 173

Query: 148 ILVVDMPPGTGDVQITMSQRLL---LSGALIVSTPQDVALMDARRGIKMFSNVHVPILGI 207
            LVVD PPGT D  I++ Q LL   + GA+IV+TPQ+V+L+D R+ +     V VP+LG+
Sbjct: 174 YLVVDAPPGTSDEHISIVQYLLPTGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGV 233

Query: 208 VENMSYFTCPHCDER-----------------------------------SYIF--GRGG 253
           VENMS  + P  D +                                   S +F    GG
Sbjct: 234 VENMSGLSQPLKDVKFMKLATETGSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGG 293

BLAST of Cp4.1LG05g01330 vs. TAIR 10
Match: AT5G24020.1 (septum site-determining protein (MIND) )

HSP 1 Score: 56.2 bits (134), Expect = 5.1e-08
Identity = 56/258 (21.71%), Postives = 111/258 (43.02%), Query Frame = 0

Query: 36  IAIASGKGGVGKSTTAVNLAVALANKCHLNVGLLDADVYGPNVPIMMKIHQKPELTEDRK 95
           + I SGKGGVGK+TT  N+ ++LA +   +V  +DAD+   N+ +++ +  +   T    
Sbjct: 61  VVITSGKGGVGKTTTTANVGLSLA-RYGFSVVAIDADLGLRNLDLLLGLENRVNYT---- 120

Query: 96  MVPVESYGVKCMSMGLLVENNAALVWRGPMVSSALEKMTRG-----VAW----------G 155
              VE     C     LV +     +    +S    K+  G     + W          G
Sbjct: 121 --CVEVINGDCRLDQALVRDKRWSNFELLCISKPRSKLPMGFGGKALEWLVDALKTRPEG 180

Query: 156 DLDILVVDMPPGTGDVQITMSQRLLLSGALIVSTPQDVALMDARRGIKMFSNVHVPILGI 215
             D +++D P G     IT       + A++V+TP   AL DA R   +     +  + +
Sbjct: 181 SPDFIIIDCPAGIDAGFITAITP--ANEAVLVTTPDITALRDADRVTGLLECDGIRDIKM 240

Query: 216 VENMSYFTCPHCDERSYIFGRGGSRKAADEMGIPFVGEVPLEEKNRKGCDEGVPIVMSEP 275
           + N         ++   +          + +G+  +G +P + +  +  + G P+V+++P
Sbjct: 241 IVNRVRTDMIKGEDMMSVL------DVQEMLGLSLLGVIPEDSEVIRSTNRGFPLVLNKP 300

Query: 276 NSLVSTKYVEIAEKVMER 279
            +L    + + A +++E+
Sbjct: 301 PTLAGLAFEQAAWRLVEQ 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54F157.7e-7849.47Iron-sulfur protein NUBPL OS=Dictyostelium discoideum OX=44689 GN=nubpl PE=3 SV=... [more]
Q8TB372.9e-7755.08Iron-sulfur protein NUBPL OS=Homo sapiens OX=9606 GN=NUBPL PE=1 SV=3[more]
Q9CWD81.9e-7654.86Iron-sulfur protein NUBPL OS=Mus musculus OX=10090 GN=Nubpl PE=1 SV=2[more]
O249996.1e-6748.45Iron-sulfur cluster carrier protein OS=Helicobacter pylori (strain ATCC 700392 /... [more]
Q9ZMM51.0e-6648.45Iron-sulfur cluster carrier protein OS=Helicobacter pylori (strain J99 / ATCC 70... [more]
Match NameE-valueIdentityDescription
XP_023534279.17.23e-212100.00iron-sulfur protein NUBPL isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6606394.11.20e-21099.32Iron-sulfur protein NUBPL, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022931224.16.93e-21098.98iron-sulfur protein NUBPL isoform X1 [Cucurbita moschata][more]
XP_022995177.13.04e-20697.96iron-sulfur protein NUBPL isoform X1 [Cucurbita maxima][more]
XP_038888219.12.08e-18990.14iron-sulfur protein NUBPL isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1ETQ43.36e-21098.98iron-sulfur protein NUBPL isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114374... [more]
A0A6J1JY281.47e-20697.96iron-sulfur protein NUBPL isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490799... [more]
A0A5A7UNF73.48e-18788.78Iron-sulfur protein NUBPL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BLW53.48e-18788.78iron-sulfur protein NUBPL OS=Cucumis melo OX=3656 GN=LOC103491273 PE=3 SV=1[more]
A0A6J1CW384.94e-18789.46iron-sulfur protein NUBPL OS=Momordica charantia OX=3673 GN=LOC111015332 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G19540.11.7e-10168.09IND1(iron-sulfur protein required for NADH dehydrogenase)-like [more]
AT3G24430.15.0e-4839.84ATP binding [more]
AT5G50960.16.1e-3834.94nucleotide binding protein 35 [more]
AT5G24020.15.1e-0821.71septum site-determining protein (MIND) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 27..290
e-value: 1.1E-92
score: 312.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 34..287
IPR033756Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35PFAMPF10609ParAcoord: 32..276
e-value: 2.1E-99
score: 332.0
IPR044304Iron-sulfur protein NUBPL-likePANTHERPTHR42961IRON-SULFUR PROTEIN NUBPLcoord: 23..285
IPR019591Mrp/NBP35 ATP-binding proteinHAMAPMF_02040Mrp_NBP35coord: 27..276
score: 36.288048
NoneNo IPR availableCDDcd02037Mrp_NBP35coord: 34..242
e-value: 1.27654E-113
score: 324.84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g01330.1Cp4.1LG05g01330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0032981 mitochondrial respiratory chain complex I assembly
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0051536 iron-sulfur cluster binding