Cp4.1LG05g00710 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG05g00710
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	AvrRpt-cleavage domain-containing protein
Location	Cp4.1LG05: 640818 .. 642964 (+)
RNA-Seq Expression	Cp4.1LG05g00710
Synteny	Cp4.1LG05g00710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAGCCACGTGTAATCTCTGTGGGAGAGGGTAGAGGAGACGAAGACTTCTACAGTTGCTACTGCTCTGGTCTTTTTTTCATTTTCTGCTTTATTTATTAACAATGGAGGTTCCTTCATCACGAAGCCAATTCTCTCTCTCTTTCTCTCTCTAAACTCATCGCTTCCGTATGTTCCTCTTCTTCCTCGGATTCCGGTGATCCTCTTCGCCGTGATTTCTCGTCGGAACTCGAGTCTGCTCGTAGTCCGGCAGTTTTTTCCCATTTCGAAGTAGTTCCCGCCGCCGCGCGCGTCTGACCCCGGTGGATAAACCACCATGGCTGTAAGCTTCCATTTCTCTTCTTTTGTTTATTTGAATGTTTCAGTGTGCGTTTTCTTTGTTATGTTCTGTTTGGCTGCTTGGAAAATATCATGGTTAGTGTGAAAACAGAGAATGAGAATTCAAAATTTGCTTTCCGACTGGAATTAAACGAGTTGCGTTTTCTCATTTCATTTGCCCTAAAGTGATGTGAGAGATCTAAATCAAATTTCAGGCTCTGTTGAATGGCGATGGAAATCGAGAAACGGTTATTGGAACCGAATAGTTTTTGAGGATTGAATCAATTTGGAGAACTGTTCAAAAGCTTTCTAGATGTTATTTAGTCTGAGCGATTTAAAAATGGTTTTCACTTTCATCGTTTTCATACGGACGAAATTATTGCTAGTTTTCAATGCTTTTTGCTTCTGTTTGTTCATATGCTAGTGGTGGGTGCCACTTTTTCTTGTAGAAATGTTCAATCACGAGCATCTGATTCACACTCAGAATTAAATTAATACTACTTTTGCCATTCTACTTAATCATTATAAGAAAGATGCATACATCCTTCCATATAAAACAATATCCTTGTACAGATGATAAACAAGTTGAGCGAGCTTATTTAGAATACATGTGGGGCTTTATTTTATTTTATTGAGCTCCCTTTTTGAGTATAGGGACATTCAAGTATTCTCTCAAACAATCATCTGTTCATTAATTCTTTCTAATTCTGCTTTATATTTTGAACTTTATGGAAAAGGTCTAGTCTCTGAATATGTGATTTTTGTCGAATTCTTAACAAATTCCTATAAACCAAGCTCTATTCTCCATCTTCTCAATGGCGTTTAAGTTTTTACATTGCCCATAACTTAGGGAAACACTAAAAAATCTCTGTTTCCTTTTTTGTTTTCATTTCACATTTGTTGTTTTATGATTGGACTCTTTATAATAGCTGTTTTGACATTTTGGTTCAAATTGCTAGATAGCGTAGATCCCATGAAGGCAAAGGAAAATCTGTTTGGATTAACATTATGCATTTCATCTCTAGCCTTAGGCACTAATTATGGATGGAATTCTTTTGAGTGCCCCCCACTTTTTCAAACAAATGAGCAATTTTCCAAACTTGTGAGGGAATGGAAACCTTCTTGTTTTTGCTGAAATTGTCAAAGTTGCCGTCTGTTCTTTTGGGATCAGTTGGTAAATATGGATATGGATATGGATGATATTGATTCTTATTCAAAGACTTATATTTGTGGTGCATCTTTTAGGTCAATTGTAGGTAATGATCATATATGGTTCATACATTAGAGCTATCTCAAGCACGTAGAAGTGAGGATTTAAAATCTTGAACATTTTGGTTGATAGTATAGGTGAGATCCCACGTTGGTTGGTTGGTTGGTTGGTTGGGGAGGAGGGTGAAACATTTTTTTATAAGAGTGTGGAAACCTCTCCCTACCAGATGCGTTTTAGAAACCTTGAGGAAAAATGACAATATGTTCTAGCGGTGGACTTGGGCTATTAGAGCATATGTCTGAACTAGAACTAGATGAATTAATGTTCAAATTGACATAGCTATATTTCTTTGTTCATAATCGATACGGAACTGAAATGGTTGCAAACTAACCAAAGGACAAAGAAGTTGCGGTTCCAAAATTTGGTGAATGGAACGACAGGGACGCCACACCACCAGACAACTACACAGCTATTTTCACCAAGGTGCGGGAAGAGAGACAAGACCAGGGAGCAAGGACGCCGACGCTGCCACAGAACAGTAACATCCCAGATAACCAAAATGATGAACAAAATCAGAAGGTAATTGAAGAACTCTCTTCCATTTCTCAGCCAACCACGTGA

mRNA sequence

TCAGCCACGTGTAATCTCTGTGGGAGAGGGTAGAGGAGACGAAGACTTCTACAGTTGCTACTGCTCTGGTCTTTTTTTCATTTTCTGCTTTATTTATTAACAATGGAGGTTCCTTCATCACGAAGCCAATTCTCTCTCTCTTTCTCTCTCTAAACTCATCGCTTCCGTATGTTCCTCTTCTTCCTCGGATTCCGGTGATCCTCTTCGCCGTGATTTCTCGTCGGAACTCGAGTCTGCTCGTAGTCCGGCAGTTTTTTCCCATTTCGAAGTAGTTCCCGCCGCCGCGCGCGTCTGACCCCGGTGGATAAACCACCATGGCTGACAAAGAAGTTGCGGTTCCAAAATTTGGTGAATGGAACGACAGGGACGCCACACCACCAGACAACTACACAGCTATTTTCACCAAGGTGCGGGAAGAGAGACAAGACCAGGGAGCAAGGACGCCGACGCTGCCACAGAACAGTAACATCCCAGATAACCAAAATGATGAACAAAATCAGAAGGTAATTGAAGAACTCTCTTCCATTTCTCAGCCAACCACGTGA

Coding sequence (CDS)

ATGGCTGACAAAGAAGTTGCGGTTCCAAAATTTGGTGAATGGAACGACAGGGACGCCACACCACCAGACAACTACACAGCTATTTTCACCAAGGTGCGGGAAGAGAGACAAGACCAGGGAGCAAGGACGCCGACGCTGCCACAGAACAGTAACATCCCAGATAACCAAAATGATGAACAAAATCAGAAGGTAATTGAAGAACTCTCTTCCATTTCTCAGCCAACCACGTGA

Protein sequence

MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQNQKVIEELSSISQPTT

Homology

[Close]

BLAST of Cp4.1LG05g00710 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.5e-04
Identity = 26/58 (44.83%), Postives = 35/58 (60.34%), Query Frame = 0


Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQ-----DQGARTPTLPQNSNIPDN 56
           +K   VPKFG+W++ + +  D YT IF KVREER         +RTPT  Q+S  P+N
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPT-HQSSRNPNN 199

[Close]

BLAST of Cp4.1LG05g00710 vs. NCBI nr
Match: KAG7036250.1 (hypothetical protein SDJN02_03052 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 134 bits (338), Expect = 4.67e-39
Identity = 64/68 (94.12%), Postives = 65/68 (95.59%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60
          MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ
Sbjct: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60

Query: 61 NQKVIEEL 68
          NQKV   +
Sbjct: 61 NQKVCSSM 68

[Close]

BLAST of Cp4.1LG05g00710 vs. NCBI nr
Match: KAG6606311.1 (RPM1-interacting protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 132 bits (333), Expect = 1.19e-38
Identity = 63/63 (100.00%), Postives = 63/63 (100.00%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60
          MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ
Sbjct: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60

Query: 61 NQK 63
          NQK
Sbjct: 61 NQK 63

[Close]

BLAST of Cp4.1LG05g00710 vs. NCBI nr
Match: XP_038888923.1 (RPM1-interacting protein 4-like [Benincasa hispida])

HSP 1 Score: 78.6 bits (192), Expect = 3.88e-17
Identity = 40/64 (62.50%), Postives = 47/64 (73.44%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQN-SNIPDNQNDE 60
          MAD E  VPKFGEW+DRDA  P+NYTAIF KVRE RQ+ G R  T P++ +N  +NQN E
Sbjct: 1  MADNEATVPKFGEWDDRDAKAPENYTAIFKKVREGRQEPGIRALTPPRSINNSTNNQNHE 60

Query: 61 QNQK 63
           NQK
Sbjct: 61 HNQK 64

[Close]

BLAST of Cp4.1LG05g00710 vs. NCBI nr
Match: KAF2284528.1 (hypothetical protein GH714_026145 [Hevea brasiliensis])

HSP 1 Score: 59.7 bits (143), Expect = 2.38e-09
Identity = 26/52 (50.00%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNI 52
          M DK  AVPKFGEW++ +    D YT IF KVREERQ +  + P +P  S++
Sbjct: 34 MPDKGAAVPKFGEWDENNPASADGYTHIFNKVREERQIEAGKVPGMPTESSM 85

[Close]

BLAST of Cp4.1LG05g00710 vs. NCBI nr
Match: XP_022887205.1 (RPM1-interacting protein 4-like [Olea europaea var. sylvestris] >CAA2935327.1 Hypothetical predicted protein [Olea europaea subsp. europaea])

HSP 1 Score: 59.3 bits (142), Expect = 2.57e-08
Identity = 26/45 (57.78%), Postives = 29/45 (64.44%), Query Frame = 0


Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLP 47
           D   AVPKFGEW+D D      Y+ IFTKVREERQ  G R P +P
Sbjct: 156 DDSPAVPKFGEWDDTDPAAAAGYSGIFTKVREERQSGGGRVPAMP 200

[Close]

BLAST of Cp4.1LG05g00710 vs. ExPASy TrEMBL
Match: A0A0A0L950 (AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G630240 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 8.59e-17
Identity = 43/69 (62.32%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGAR-TPTLPQNSNIPDNQNDE 60
          MAD E AVPKFGEW+D DA  P+NYTAIF KVREERQDQ  R TPT   +S   ++QN E
Sbjct: 1  MADNETAVPKFGEWDDGDAKAPENYTAIFNKVREERQDQTVRGTPTRLIDST--NSQNHE 60

Query: 61 QNQKVIEEL 68
          QNQK+ +++
Sbjct: 61 QNQKLGQKI 67

[Close]

BLAST of Cp4.1LG05g00710 vs. ExPASy TrEMBL
Match: A0A6A6K948 (AvrRpt-cleavage domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_026145 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.15e-09
Identity = 26/52 (50.00%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNI 52
          M DK  AVPKFGEW++ +    D YT IF KVREERQ +  + P +P  S++
Sbjct: 34 MPDKGAAVPKFGEWDENNPASADGYTHIFNKVREERQIEAGKVPGMPTESSM 85

[Close]

BLAST of Cp4.1LG05g00710 vs. ExPASy TrEMBL
Match: A0A2C9UP29 (AvrRpt-cleavage domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_13G048800 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 6.13e-08
Identity = 26/50 (52.00%), Postives = 32/50 (64.00%), Query Frame = 0


Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNI 52
           DK  AVPKFGEW++ +    D YT IF KVREERQ +  + P  P  S+I
Sbjct: 194 DKGAAVPKFGEWDENNPASADGYTHIFNKVREERQIEAGKVPGTPTRSSI 243

[Close]

BLAST of Cp4.1LG05g00710 vs. ExPASy TrEMBL
Match: A0A7I8L7E9 (Hypothetical protein OS=Spirodela intermedia OX=51605 GN=SI8410_11015916 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.01e-07
Identity = 24/53 (45.28%), Postives = 34/53 (64.15%), Query Frame = 0


Query: 3  DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDN 55
          +K   VPKFG+W++ D T  DN+T IF KVR+ER+D G + P +  +    DN
Sbjct: 6  EKRSTVPKFGDWDENDPTSADNFTGIFKKVRDERKD-GGKPPMISTDPEFEDN 57

[Close]

BLAST of Cp4.1LG05g00710 vs. ExPASy TrEMBL
Match: M1CPC8 (RIN4c protein OS=Solanum tuberosum OX=4113 GN=102596147 PE=4 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.08e-07
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0


Query: 1   MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQGARTPTLPQNSNIPDNQNDEQ 60
            +D+  AVP+FGEW++ D    DNYT IF KVREE+Q   + TP+   N+    N  ++Q
Sbjct: 189 QSDRGAAVPRFGEWDENDPQSADNYTHIFNKVREEKQGNPSGTPSRASNNTQKHNSEEKQ 248

[Close]

BLAST of Cp4.1LG05g00710 vs. TAIR 10
Match: AT3G25070.1 (RPM1 interacting protein 4 )

HSP 1 Score: 46.6 bits (109), Expect = 1.0e-05
Identity = 26/58 (44.83%), Postives = 35/58 (60.34%), Query Frame = 0


Query: 3   DKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQ-----DQGARTPTLPQNSNIPDN 56
           +K   VPKFG+W++ + +  D YT IF KVREER         +RTPT  Q+S  P+N
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPT-HQSSRNPNN 199

[Close]

BLAST of Cp4.1LG05g00710 vs. TAIR 10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQG 41
          MADK   +PKFGEW+  D +  + +T IF K R E++  G
Sbjct: 1  MADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGG 40

[Close]

BLAST of Cp4.1LG05g00710 vs. TAIR 10
Match: AT5G55850.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 41.6 bits (96), Expect = 3.3e-04
Identity = 17/40 (42.50%), Postives = 25/40 (62.50%), Query Frame = 0


Query: 1  MADKEVAVPKFGEWNDRDATPPDNYTAIFTKVREERQDQG 41
          M+DK   +PKFGEW+  D    + +T IF K R+E++  G
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGG 40

The following BLAST results are available for this feature:

BLAST of Cp4.1LG05g00710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYN5	1.5e-04	44.83	RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1	[more]

BLAST of Cp4.1LG05g00710 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7036250.1	4.67e-39	94.12	hypothetical protein SDJN02_03052 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6606311.1	1.19e-38	100.00	RPM1-interacting protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038888923.1	3.88e-17	62.50	RPM1-interacting protein 4-like [Benincasa hispida]	[more]
KAF2284528.1	2.38e-09	50.00	hypothetical protein GH714_026145 [Hevea brasiliensis]	[more]
XP_022887205.1	2.57e-08	57.78	RPM1-interacting protein 4-like [Olea europaea var. sylvestris] >CAA2935327.1 Hy...	[more]

BLAST of Cp4.1LG05g00710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L950	8.59e-17	62.32	AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G63...	[more]
A0A6A6K948	1.15e-09	50.00	AvrRpt-cleavage domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714...	[more]
A0A2C9UP29	6.13e-08	52.00	AvrRpt-cleavage domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_...	[more]
A0A7I8L7E9	1.01e-07	45.28	Hypothetical protein OS=Spirodela intermedia OX=51605 GN=SI8410_11015916 PE=4 SV...	[more]
M1CPC8	1.08e-07	43.33	RIN4c protein OS=Solanum tuberosum OX=4113 GN=102596147 PE=4 SV=1	[more]

BLAST of Cp4.1LG05g00710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25070.1	1.0e-05	44.83	RPM1 interacting protein 4	[more]
AT2G04410.1	2.0e-04	45.00	RPM1-interacting protein 4 (RIN4) family protein	[more]
AT5G55850.1	3.3e-04	42.50	RPM1-interacting protein 4 (RIN4) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008700	RIN4, pathogenic type III effector avirulence factor Avr cleavage site	PFAM	PF05627	AvrRpt-cleavage	coord: 3..37 e-value: 2.0E-13 score: 49.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..76
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 41..76
None	No IPR available	PANTHER	PTHR33159:SF26	RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN	coord: 2..63
IPR040387	RPM1-interacting protein 4/NOI4	PANTHER	PTHR33159	RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN	coord: 2..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG05g00710.1	Cp4.1LG05g00710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane