Cp4.1LG05g00510 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g00510
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
LocationCp4.1LG05: 697732 .. 699873 (-)
RNA-Seq ExpressionCp4.1LG05g00510
SyntenyCp4.1LG05g00510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATGAAACTAAAATATTCCATATTTGTCCCCAACGAACATTGACGAGCACACTCCTCTTTCTTCATCTTCTATATCACCATCCTTCCTCAATCCCTACTCTCTTCATTTCCGCCATTGTTACTCAGCTTCTGCAACAATGGCGATTCCCTTCTCCTCTCTTCTCTTAGCATTCTACTTTCTCTCACTAACAAGTATTCTCACTCCCCTTCTTCATTCTTCATTCTTCCTCCATTTCTCTGTTTCTCTGTTTCTGTTTCTCTCTAATCTTCTCCACATTCCTTTCTTGCAGGTGCAAAGATCTCCACTCTCGTCGGAATCAACTACGGCCAGCTCGGCAACAACCTCCCTCCGCCGCCCGATTCCGTCGATTTGATCAAATCCCTAAACGCCCAAATCGTTAAAATCTATGATGTCAATCCCCAAATCCTCAAATCCCTAAACAACTCTGATATCAAGGTATCCGTCATGGTCCCTAACGATCTTATCATCAACATTTCGTCTAATCAGAACCTCGCCGATCACTGGGTTCGTACCAATATCCTCCCGTTTTACCCACAGATCAAAATCCGGTACCTCCTTGTCGGAAACGAAATCATCTCCTCCACCGGCAACCAAACCTGGATCAGTTTGGTGCCGGCGATGCGCCGAATCAAACGCTCCTTGAAAATTTATGGGATTCATAAAGTGAAAGTTGGAACCTCCTCCGCCATGGATGTTCTTCAATCGTCGTTTCCGCCGTCGAACGGGACTTTTCGGGCGGATATTTCCGATCGGGTCATGAAACCGATGTTGCAGTTTCTGAACCGGACGAAATCGTTCTTCTTCCTCGATGTTTACCCGTTTTTCCCTTGGGCTTCCGATCCTGTCAACATCAAACTCGATTACGCGCTATTTGAATCGAAGAACATCACGTATTCGGATCCGGGTTCGGGTTTGACCTACACGAATCTCTTCGATCAGATGGTGGATTCGGTGATTTTCGCGATGAAACGTCTTGGGTACCCGGATATTCGGATCTTCATCGCCGAAACCGGCTGGCCCAACGGTGGCGATTACGACCAAATCGGCGCCAACATCCACAACGCCGCCGTGTACAACCGCAATATCGTGAAGCGCGTCACTGCCAAGCCGCCAGTGGGGACTCCAGCTCGACCGGGTTGGGTTCTGCCGACGATTATATTCTCTCTGTATAACGAGAATCAGAAACCGGGTCTGGGTACAGAGAGGCATTTCGGCCTATTATACCCAAACCGGTCGGCCGTGTATGAGATGGATTTGAGTGGGAAGACGCCGGAGGCAGATTTTAAGCCGCTACCGGCGGCGGAGACGAACGAGCCGTACAAGGGGAAGATTTGGTGCATGGTATCAAGGGGGGCCAATAAGAGTGAGGTTGCAGGGGCATTAGCGTGGGCTTGTTTACAAGGGAATAAAACCTGTGAACCGATACAGAAAGGCGGTCCGTGTTACGAACCGGATTCTTTGATACGACATGCGAGCTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTTGGTGGGACCTGTTACTTTAACGGGCTGGCCACTCAGACCATCAAGGACCCAAGTAAGTTGATTTTATTTTATTTATTTATATTTTTAAAAATTCTAAATATTTTATTTATTAATTAAATTTTAAATTTTATTTCTCAGGTTATGGAAAGTGCAAGTTCCCGAGCGTCACTCTGCAAGGATGATGACGTAATAAAGAAATTTTAATGAGTTTTATTTTTCGTTTATGTTTTTATTTTTTATTTTTAAAATTTAAATTTCATGGTTGATTTAGGAGGGTAGTTTGGGCATCCCATTTCCCTAGAAGTATTAACTACGGAATGGCTGAAGACTAGTTTTATTTTATTTTATTTTATTTTTATTTTTATTTTGTTGGTAAGAAAGAAATGGGCGCCCAAATCACCTTTCCATTTTCACTAATTCCACTCACTATTTTTCTGACATCTTTTGCCACGCGTGCAACCACCTTTTCAACGCCATTTGTATGTCCACAACGGCTATTTTTTGTACCAGCAAATATGAAAAATTCAATATTGGTTAATATATATAATTAATAACCGTTTAATTTTTTAAAATTTTAGATAGGGGTATGAATAAATTTATAAAT

mRNA sequence

TAATGAAACTAAAATATTCCATATTTGTCCCCAACGAACATTGACGAGCACACTCCTCTTTCTTCATCTTCTATATCACCATCCTTCCTCAATCCCTACTCTCTTCATTTCCGCCATTGTTACTCAGCTTCTGCAACAATGGCGATTCCCTTCTCCTCTCTTCTCTTAGCATTCTACTTTCTCTCACTAACAAGTGCAAAGATCTCCACTCTCGTCGGAATCAACTACGGCCAGCTCGGCAACAACCTCCCTCCGCCGCCCGATTCCGTCGATTTGATCAAATCCCTAAACGCCCAAATCGTTAAAATCTATGATGTCAATCCCCAAATCCTCAAATCCCTAAACAACTCTGATATCAAGGTATCCGTCATGGTCCCTAACGATCTTATCATCAACATTTCGTCTAATCAGAACCTCGCCGATCACTGGGTTCGTACCAATATCCTCCCGTTTTACCCACAGATCAAAATCCGGTACCTCCTTGTCGGAAACGAAATCATCTCCTCCACCGGCAACCAAACCTGGATCAGTTTGGTGCCGGCGATGCGCCGAATCAAACGCTCCTTGAAAATTTATGGGATTCATAAAGTGAAAGTTGGAACCTCCTCCGCCATGGATGTTCTTCAATCGTCGTTTCCGCCGTCGAACGGGACTTTTCGGGCGGATATTTCCGATCGGGTCATGAAACCGATGTTGCAGTTTCTGAACCGGACGAAATCGTTCTTCTTCCTCGATGTTTACCCGTTTTTCCCTTGGGCTTCCGATCCTGTCAACATCAAACTCGATTACGCGCTATTTGAATCGAAGAACATCACGTATTCGGATCCGGGTTCGGGTTTGACCTACACGAATCTCTTCGATCAGATGGTGGATTCGGTGATTTTCGCGATGAAACGTCTTGGGTACCCGGATATTCGGATCTTCATCGCCGAAACCGGCTGGCCCAACGGTGGCGATTACGACCAAATCGGCGCCAACATCCACAACGCCGCCGTGTACAACCGCAATATCGTGAAGCGCGTCACTGCCAAGCCGCCAGTGGGGACTCCAGCTCGACCGGGTTGGGTTCTGCCGACGATTATATTCTCTCTGTATAACGAGAATCAGAAACCGGGTCTGGGTACAGAGAGGCATTTCGGCCTATTATACCCAAACCGGTCGGCCGTGTATGAGATGGATTTGAGTGGGAAGACGCCGGAGGCAGATTTTAAGCCGCTACCGGCGGCGGAGACGAACGAGCCGTACAAGGGGAAGATTTGGTGCATGGTATCAAGGGGGGCCAATAAGAGTGAGGTTGCAGGGGCATTAGCGTGGGCTTGTTTACAAGGGAATAAAACCTGTGAACCGATACAGAAAGGCGGTCCGTGTTACGAACCGGATTCTTTGATACGACATGCGAGCTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTTGGTGGGACCTGTTACTTTAACGGGCTGGCCACTCAGACCATCAAGGACCCAAGTTATGGAAAGTGCAAGTTCCCGAGCGTCACTCTGCAAGGATGATGACGTAATAAAGAAATTTTAATGAGTTTTATTTTTCGTTTATGTTTTTATTTTTTATTTTTAAAATTTAAATTTCATGGTTGATTTAGGAGGGTAGTTTGGGCATCCCATTTCCCTAGAAGTATTAACTACGGAATGGCTGAAGACTAGTTTTATTTTATTTTATTTTATTTTTATTTTTATTTTGTTGGTAAGAAAGAAATGGGCGCCCAAATCACCTTTCCATTTTCACTAATTCCACTCACTATTTTTCTGACATCTTTTGCCACGCGTGCAACCACCTTTTCAACGCCATTTGTATGTCCACAACGGCTATTTTTTGTACCAGCAAATATGAAAAATTCAATATTGGTTAATATATATAATTAATAACCGTTTAATTTTTTAAAATTTTAGATAGGGGTATGAATAAATTTATAAAT

Coding sequence (CDS)

ATGGCGATTCCCTTCTCCTCTCTTCTCTTAGCATTCTACTTTCTCTCACTAACAAGTGCAAAGATCTCCACTCTCGTCGGAATCAACTACGGCCAGCTCGGCAACAACCTCCCTCCGCCGCCCGATTCCGTCGATTTGATCAAATCCCTAAACGCCCAAATCGTTAAAATCTATGATGTCAATCCCCAAATCCTCAAATCCCTAAACAACTCTGATATCAAGGTATCCGTCATGGTCCCTAACGATCTTATCATCAACATTTCGTCTAATCAGAACCTCGCCGATCACTGGGTTCGTACCAATATCCTCCCGTTTTACCCACAGATCAAAATCCGGTACCTCCTTGTCGGAAACGAAATCATCTCCTCCACCGGCAACCAAACCTGGATCAGTTTGGTGCCGGCGATGCGCCGAATCAAACGCTCCTTGAAAATTTATGGGATTCATAAAGTGAAAGTTGGAACCTCCTCCGCCATGGATGTTCTTCAATCGTCGTTTCCGCCGTCGAACGGGACTTTTCGGGCGGATATTTCCGATCGGGTCATGAAACCGATGTTGCAGTTTCTGAACCGGACGAAATCGTTCTTCTTCCTCGATGTTTACCCGTTTTTCCCTTGGGCTTCCGATCCTGTCAACATCAAACTCGATTACGCGCTATTTGAATCGAAGAACATCACGTATTCGGATCCGGGTTCGGGTTTGACCTACACGAATCTCTTCGATCAGATGGTGGATTCGGTGATTTTCGCGATGAAACGTCTTGGGTACCCGGATATTCGGATCTTCATCGCCGAAACCGGCTGGCCCAACGGTGGCGATTACGACCAAATCGGCGCCAACATCCACAACGCCGCCGTGTACAACCGCAATATCGTGAAGCGCGTCACTGCCAAGCCGCCAGTGGGGACTCCAGCTCGACCGGGTTGGGTTCTGCCGACGATTATATTCTCTCTGTATAACGAGAATCAGAAACCGGGTCTGGGTACAGAGAGGCATTTCGGCCTATTATACCCAAACCGGTCGGCCGTGTATGAGATGGATTTGAGTGGGAAGACGCCGGAGGCAGATTTTAAGCCGCTACCGGCGGCGGAGACGAACGAGCCGTACAAGGGGAAGATTTGGTGCATGGTATCAAGGGGGGCCAATAAGAGTGAGGTTGCAGGGGCATTAGCGTGGGCTTGTTTACAAGGGAATAAAACCTGTGAACCGATACAGAAAGGCGGTCCGTGTTACGAACCGGATTCTTTGATACGACATGCGAGCTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTTGGTGGGACCTGTTACTTTAACGGGCTGGCCACTCAGACCATCAAGGACCCAAGTTATGGAAAGTGCAAGTTCCCGAGCGTCACTCTGCAAGGATGA

Protein sequence

MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDRVMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPLPAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Homology
BLAST of Cp4.1LG05g00510 vs. ExPASy Swiss-Prot
Match: Q06915 (Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=A6 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 4.8e-151
Identity = 244/451 (54.10%), Postives = 333/451 (73.84%), Query Frame = 0

Query: 13  YFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQILKSLNNSD 72
           Y    T   +++ +GINYG+ GNNLP P  S++ IKS+ A  VK+YD +P+ L  L+ ++
Sbjct: 29  YMQRKTMLDLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTN 88

Query: 73  IKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISSTGNQTWISL 132
           + V++ VPN  I  +SSNQ +AD WVRTNILP+YPQ +IR++LVGNEI+S       ++L
Sbjct: 89  LYVTITVPNHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNL 148

Query: 133 VPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDRVMKPMLQFLNRT 192
           VPAMR+I  SL+++GIH +KVGT  AMD L+SSFPPSNGTFR +I+  VM P+L+FLN T
Sbjct: 149 VPAMRKIVNSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGT 208

Query: 193 KSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQMVDSVIFAMK 252
            S+FFL+V+P+F W+ +P+N  LD+ALF+  + TY+DP +GL Y NL DQM+DSV+FAM 
Sbjct: 209 NSYFFLNVHPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMT 268

Query: 253 RLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVGTPARPGWVLP 312
           +LGYP +R+ I+ETGWPN GD D+ GANI NAA YNRN++K+++A PP+GTP+RPG  +P
Sbjct: 269 KLGYPHMRLAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIP 328

Query: 313 TIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPLPAAETNEPYKGK 372
           T +FSL+NENQK G GT+RH+G+L+P+ S +Y++D +G+TP   F PLP    N PYKG+
Sbjct: 329 TFVFSLFNENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQ 388

Query: 373 IWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHASFAFSSYWAQFR 432
           +WC+   GAN++E+   L  AC Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR
Sbjct: 389 VWCVPVEGANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFR 448

Query: 433 KVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
                C+FNGLA +T  +P   +CKFPSVTL
Sbjct: 449 NQSIQCFFNGLAHETTTNPGNDRCKFPSVTL 478

BLAST of Cp4.1LG05g00510 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 7.9e-93
Identity = 176/458 (38.43%), Postives = 266/458 (58.08%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MA  F  +      L L        VG+ YG+  ++LP P   V LI+  N + V+IYD 
Sbjct: 1   MARDFKLIFSISILLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDY 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           N Q+LK+  N+ I++ + VPN  +   S +Q+  D W++ ++LP+YP  KI Y+ VG E 
Sbjct: 61  NSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAES 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGI-HKVKVGTSSAMDVLQSSFPPSNGTFRADISD 180
                      +VPAM+ +  +L+  G+  ++KV T+ ++ +L  SFPPS G F +  + 
Sbjct: 121 TDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA- 180

Query: 181 RVMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNL 240
             ++PML+FL   KS F +D+YP++ +   P N+ LDY LFES +    DP +GL Y N+
Sbjct: 181 YFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSS-EVIDPNTGLLYKNM 240

Query: 241 FDQMVDSVIFAMKRLGYPDIRIFIAETGWP-NGGDYDQIGANIHNAAVYNRNIVKRVTAK 300
           FD  VD++ +A+  L +  I+I + ETGWP  G   ++  A+  NA  YN NI++ V   
Sbjct: 241 FDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTN 300

Query: 301 PPVGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFK 360
              GTPA+PG  +   IFSL+NEN+K GL +ER++GL YP++++VY++D +GK+      
Sbjct: 301 Q--GTPAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSN 360

Query: 361 PLPAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIR 420
              ++ TN       WC+ S  A++ ++ GAL WAC  GN  C  IQ   PC++PD+L+ 
Sbjct: 361 ---SSGTNSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVS 420

Query: 421 HASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKC 457
           HASF F+SY+ Q R     C F G   +  KDPSY KC
Sbjct: 421 HASFVFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKC 451

BLAST of Cp4.1LG05g00510 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 9.0e-89
Identity = 167/465 (35.91%), Postives = 270/465 (58.06%), Query Frame = 0

Query: 15  LSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQILKSLNNSDIK 74
           L+  +  +++ +GI YG+  +NLP P    +LI+ LN + V+IYD N  +LK+  N+ I+
Sbjct: 16  LAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIE 75

Query: 75  VSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISSTGNQTWISLVP 134
           + + VPN  ++  +  Q+  D W+  NILP+YP  KI  + VG E+  +  N T + ++P
Sbjct: 76  LMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGL-VLP 135

Query: 135 AMRRIKRSLKIYGI-HKVKVGTSSAMDVLQSSFPPSNGTFRADISDRVMKPMLQFLNRTK 194
           AMR I  +LK  G+  K+K+ +S ++ +L  SFPPS+ +F    S   +KPML+FL   +
Sbjct: 136 AMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHS-AFLKPMLEFLVENE 195

Query: 195 SFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQMVDSVIFAMKR 254
           S F +D+YP++ +      + L+YALFES +    DP +GL Y+N+FD  +D++ FA+  
Sbjct: 196 SPFMIDLYPYYAYRDSTEKVPLEYALFESSS-QVVDPATGLLYSNMFDAQLDAIYFALTA 255

Query: 255 LGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVGTPARPGWVLPT 314
           + +  +++ + E+GWP+ G   +  A   NA  YN N+++ V   P  GTPA+PG  +  
Sbjct: 256 MSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP--GTPAKPGEEIDV 315

Query: 315 IIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGK--------------TPEADFKP 374
            +FSL+NEN+KPG+ +ER++G+ Y N + VY +D +G+              +P     P
Sbjct: 316 YLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSP 375

Query: 375 LPAAETNEPYKG------KIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEP 434
           +    +     G      K WC+ S  A+ +E+  AL WAC  GN  C  +Q   PC+EP
Sbjct: 376 IINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEP 435

Query: 435 DSLIRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKF 459
           D+++ HAS+AF++Y+ Q       C FNG + +  KDPSYG C +
Sbjct: 436 DTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of Cp4.1LG05g00510 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 322.4 bits (825), Expect = 8.4e-87
Identity = 172/464 (37.07%), Postives = 274/464 (59.05%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MA+  S   L  +     S+     +G+NYGQ+ +NLPPP ++V L++S + Q V++Y  
Sbjct: 1   MALSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGA 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           +P I+K+L  + + + +   N  + +++S+ N A  W+ +N+LPFYP  KI  + VGNEI
Sbjct: 61  DPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGI-HKVKVGTSSAMDVLQSSFPPSNGTFRADISD 180
           + S        L+PAM+ ++++L+   +  K+KV T ++M VL SS PPS+G+F A    
Sbjct: 121 LMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQT 180

Query: 181 RVMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNL 240
             +K +LQFL+ T S F ++ YPFF + SDP    L + LFE  N    D  +G+ YTN+
Sbjct: 181 G-LKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFE-PNAGRVDSKTGIKYTNM 240

Query: 241 FDQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKP 300
           FD  VD+V  A+K +G+  + I +AETGW + GD +++GA++ NA  YN N++  +  + 
Sbjct: 241 FDAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHL--RS 300

Query: 301 PVGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKP 360
            VGTP  PG  + T IF+LY+EN KPG  +ER FGL   + S VY++ L+          
Sbjct: 301 MVGTPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLA---------- 360

Query: 361 LPAAETNEPYKGKI----WCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDS 420
             ++ +++   GK+    WC+  +GA   E+  +L WAC  G   C  IQ GG C+EP++
Sbjct: 361 -KSSSSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHG-IDCGAIQPGGACFEPNN 420

Query: 421 LIRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFP 460
           ++ HA++A + Y+ +  K    C F+  AT T ++PSY  C +P
Sbjct: 421 VVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of Cp4.1LG05g00510 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.6e-85
Identity = 172/459 (37.47%), Postives = 268/459 (58.39%), Query Frame = 0

Query: 6   SSLLLAFYFL--SLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQ 65
           SSLL   + L  +  SA   + +G+NYGQL +NLP    +V+L+KS   Q V+++   P 
Sbjct: 8   SSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPA 67

Query: 66  ILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISS 125
           ++K+  N+ +++ +   N  I  ++SN N+A  +V++N++ FYP   I  + VGNE+++S
Sbjct: 68  VIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTS 127

Query: 126 TGNQTWIS-LVPAMRRIKRSLKIYGI-HKVKVGTSSAMDVLQSSFPPSNGTFRADISDRV 185
            G+Q  IS L+PAM+ ++ +L    +  KVKV T  AM VL  S+PPS+G F   + D  
Sbjct: 128 -GDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGD-T 187

Query: 186 MKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFD 245
           MK +LQF +   + F +  YP+F + + P    L + LF+  N    D G+G  YTN+FD
Sbjct: 188 MKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQ-PNAGQVDSGNGHKYTNMFD 247

Query: 246 QMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPV 305
             VD+V  A+  +G+ DI I +AETGWP+GGD +++G ++ NA  Y  N++  + +K  V
Sbjct: 248 AQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSK--V 307

Query: 306 GTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPLP 365
           GTP  PG  + T +FSLY+E++K G  +E++FGL  P+ S  Y++ L   T        P
Sbjct: 308 GTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQN---PTTP 367

Query: 366 AAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHAS 425
           A  T  P     WC+   G +  ++ G + +AC QG   C PIQ GG C+EP+++  HA+
Sbjct: 368 ATPTPTPKAAGSWCVPKPGVSDDQLTGNINYACGQG-IDCGPIQPGGACFEPNTVKAHAA 427

Query: 426 FAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           +  + Y+    +    C F+  AT T  +PSYG C FPS
Sbjct: 428 YVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPS 457

BLAST of Cp4.1LG05g00510 vs. NCBI nr
Match: XP_023532592.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. NCBI nr
Match: XP_022931078.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata])

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 463/465 (99.57%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLK YGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. NCBI nr
Match: XP_022996249.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita maxima])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 459/465 (98.71%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVG+SSAMD+LQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGSSSAMDILQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIR+FI+ETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRVFISETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEP+KGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPFKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKC FPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCMFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. NCBI nr
Match: KAG6606297.1 (putative glucan endo-1,3-beta-glucosidase A6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 460/464 (99.14%), Postives = 462/464 (99.57%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQIL+SLNNSDIKVSVMVPNDLI+NISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILRSLNNSDIKVSVMVPNDLIVNISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLK YGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464
           SFAFSSYWAQFRKVGGTCYFNGLATQ IKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQIIKDPSYGKCKFPSVTLQ 464

BLAST of Cp4.1LG05g00510 vs. NCBI nr
Match: KAG7036237.1 (putative glucan endo-1,3-beta-glucosidase A6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 916 bits (2367), Expect = 0.0
Identity = 448/451 (99.33%), Postives = 450/451 (99.78%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLG+NLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGDNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILKSLNNSDIKVSVMVPNDLI+NISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIVNISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLK YGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP 451
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP 451

BLAST of Cp4.1LG05g00510 vs. ExPASy TrEMBL
Match: A0A6J1EXC8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 PE=3 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 463/465 (99.57%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLK YGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. ExPASy TrEMBL
Match: A0A6J1K875 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=3 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 459/465 (98.71%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVG+SSAMD+LQSSFPPSNGTFRADISDR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGSSSAMDILQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLGYPDIR+FI+ETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRVFISETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           PAAETNEP+KGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA
Sbjct: 361 PAAETNEPFKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKC FPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCMFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. ExPASy TrEMBL
Match: A0A5A7VIC4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00460 PE=3 SV=1)

HSP 1 Score: 830 bits (2143), Expect = 5.77e-302
Identity = 403/465 (86.67%), Postives = 431/465 (92.69%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           M IPF SLLL  +FLSL+SA+IS+LVGINYGQLGNNLP P  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQIL +L N+D++VSVMVPN+LIINISSNQNLAD WVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVGTSSAMDVLQSSFPPSNGTFR DI+DR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VM+PMLQFLNRTKSF+FLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+T+KPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPG VLPTIIFSLYNEN+K G GTERHFGLLYP+  AVYEMDLSG T E  FKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           P AETNEPYKG+IWC+V +G N SEV GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. ExPASy TrEMBL
Match: A0A1S3CCE3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV=1)

HSP 1 Score: 830 bits (2143), Expect = 5.77e-302
Identity = 403/465 (86.67%), Postives = 431/465 (92.69%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           M IPF SLLL  +FLSL+SA+IS+LVGINYGQLGNNLP P  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQIL +L N+D++VSVMVPN+LIINISSNQNLAD WVRTNILPFYPQIKIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVGTSSAMDVLQSSFPPSNGTFR DI+DR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VM+PMLQFLNRTKSF+FLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+T+KPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPG VLPTIIFSLYNEN+K G GTERHFGLLYP+  AVYEMDLSG T E  FKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           P AETNEPYKG+IWC+V +G N SEV GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of Cp4.1LG05g00510 vs. ExPASy TrEMBL
Match: A0A0A0LBR1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 8.52e-302
Identity = 403/464 (86.85%), Postives = 428/464 (92.24%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           M IPF SLL   +FLSL+SA+ISTLVGINYGQLGNNLPPP  SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           NPQILK+L N+D++VSVMVPN+LIINISS+QNLAD WVRTNILPFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180
           ISSTGNQTW SLVPAMRRIK SLK YGIHKVKVGT SAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240
           VM+PMLQFLNRTKSFFFLDVYP+FPW+SD VNIKLDYALFESKNITYSDP SGL YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYP   AVYEMDLSG   E  FKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420
           P AE NEPYKGKIWC+V +G N S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of Cp4.1LG05g00510 vs. TAIR 10
Match: AT3G07320.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 660.6 bits (1703), Expect = 9.3e-190
Identity = 315/461 (68.33%), Postives = 380/461 (82.43%), Query Frame = 0

Query: 7   SLLLAFYFLSL----TSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNP 66
           SLLL  + LSL    + AK S   GINYGQLGNNLP P DSV+LIKSLNA+ VK+YD NP
Sbjct: 2   SLLLHLFALSLLISVSGAKFSGRPGINYGQLGNNLPSPSDSVNLIKSLNAKRVKLYDANP 61

Query: 67  QILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIIS 126
           +IL +LN +DI VSVMVPN+L++NIS + +L+D W+R+NILPFYP  KIRYLLVGNEI+S
Sbjct: 62  KILAALNGTDITVSVMVPNELLVNISKSASLSDDWIRSNILPFYPTTKIRYLLVGNEILS 121

Query: 127 STGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDRVM 186
              ++   SLVPAMR+I+RSLK  G+ KVKVGT+ A DVLQSSFPPS+G FR DIS  +M
Sbjct: 122 LPDSELKSSLVPAMRKIQRSLKSLGVKKVKVGTTLATDVLQSSFPPSSGEFREDISGLIM 181

Query: 187 KPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQ 246
           KPMLQFLNRTKSF F+DVYP+F WA DP ++ LDYA+FES N+T +DP S LTY NLFDQ
Sbjct: 182 KPMLQFLNRTKSFLFVDVYPYFAWAQDPTHVDLDYAIFESTNVTVTDPVSNLTYHNLFDQ 241

Query: 247 MVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVG 306
           M+D+ +FAMKR+GYPDIRI++AETGWPN GDYDQIGANI+NAA YNRN+VK++ A PPVG
Sbjct: 242 MIDAFVFAMKRVGYPDIRIWVAETGWPNNGDYDQIGANIYNAATYNRNVVKKLAADPPVG 301

Query: 307 TPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPLPA 366
           TPARPG VLP  +F+LYNENQK G GTERHFGLL+PN + VY +DLSGKT   +   LPA
Sbjct: 302 TPARPGKVLPAFVFALYNENQKTGPGTERHFGLLHPNGTQVYGIDLSGKTEYKE--SLPA 361

Query: 367 AETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHASF 426
            E N+ YKGKIWC+V++GAN +++  AL++AC QGN TC+PIQ+GGPC +PD  + HAS+
Sbjct: 362 PENNDLYKGKIWCVVAKGANWTQLGDALSYACSQGNNTCDPIQRGGPCQKPDLTVLHASY 421

Query: 427 AFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
           AFSSYWAQFRK+GGTC FNGLATQTIKDPSYG+C+FPSVTL
Sbjct: 422 AFSSYWAQFRKIGGTCSFNGLATQTIKDPSYGRCEFPSVTL 460

BLAST of Cp4.1LG05g00510 vs. TAIR 10
Match: AT3G23770.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 545.0 bits (1403), Expect = 5.7e-155
Identity = 253/449 (56.35%), Postives = 340/449 (75.72%), Query Frame = 0

Query: 18  TSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQILKSLNNSDIKVSV 77
           T   +++ +GINYG+LGNNLP P  S++LIK++ A  VK+YD +P+ LK L+ +++ V++
Sbjct: 29  TVLALASRIGINYGKLGNNLPFPYQSINLIKTIKAGHVKLYDADPETLKLLSTTNLYVTI 88

Query: 78  MVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISSTGN---QTWISLVP 137
           MVPN+ II+I ++Q  AD+WV TN+LPF+PQ +IR++LVGNE++S + +   Q W +LVP
Sbjct: 89  MVPNNQIISIGADQAAADNWVATNVLPFHPQTRIRFVLVGNEVLSYSSDQDKQIWANLVP 148

Query: 138 AMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDRVMKPMLQFLNRTKS 197
           AMR++  SL+  GIH +KVGT  AMD L+SSFPPS+GTFR DI+  VM P+L+FLN T S
Sbjct: 149 AMRKVVNSLRARGIHNIKVGTPLAMDALRSSFPPSSGTFREDIAVPVMLPLLKFLNGTNS 208

Query: 198 FFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQMVDSVIFAMKRL 257
           FFFLDVYP+FPW++DPVN  LD+ALFES N TY+DP +GL YTNL DQM+DSVIFAM +L
Sbjct: 209 FFFLDVYPYFPWSTDPVNNHLDFALFES-NSTYTDPQTGLVYTNLLDQMLDSVIFAMTKL 268

Query: 258 GYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVGTPARPGWVLPTI 317
           GYP+I + I+ETGWPN GD  + GANI NAA YNRN++K++TA PP+GTPAR G  +PT 
Sbjct: 269 GYPNISLAISETGWPNDGDIHETGANIVNAATYNRNLIKKMTANPPLGTPARRGAPIPTF 328

Query: 318 IFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPLPAAETNEPYKGKIW 377
           +FSL+NENQKPG GTERH+G+L P+ + +Y++D SG+   + F  LP    N P+KG +W
Sbjct: 329 LFSLFNENQKPGSGTERHWGILNPDGTPIYDIDFSGRRSFSGFDSLPKPSNNVPFKGNVW 388

Query: 378 CMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHASFAFSSYWAQFRKV 437
           C+   GA+++E+  AL +AC + N TC  +  GG CY P ++  HAS+AFSSYWAQFR  
Sbjct: 389 CVAVDGADEAELGQALNFACGRSNATCAALAPGGECYAPVTVTWHASYAFSSYWAQFRNQ 448

Query: 438 GGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
              CYFNGLA +T  +P   +CKFPSVTL
Sbjct: 449 SSQCYFNGLARETTTNPGNERCKFPSVTL 476

BLAST of Cp4.1LG05g00510 vs. TAIR 10
Match: AT4G14080.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 535.8 bits (1379), Expect = 3.4e-152
Identity = 244/451 (54.10%), Postives = 333/451 (73.84%), Query Frame = 0

Query: 13  YFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQILKSLNNSD 72
           Y    T   +++ +GINYG+ GNNLP P  S++ IKS+ A  VK+YD +P+ L  L+ ++
Sbjct: 29  YMQRKTMLDLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTN 88

Query: 73  IKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISSTGNQTWISL 132
           + V++ VPN  I  +SSNQ +AD WVRTNILP+YPQ +IR++LVGNEI+S       ++L
Sbjct: 89  LYVTITVPNHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNL 148

Query: 133 VPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDRVMKPMLQFLNRT 192
           VPAMR+I  SL+++GIH +KVGT  AMD L+SSFPPSNGTFR +I+  VM P+L+FLN T
Sbjct: 149 VPAMRKIVNSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGT 208

Query: 193 KSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQMVDSVIFAMK 252
            S+FFL+V+P+F W+ +P+N  LD+ALF+  + TY+DP +GL Y NL DQM+DSV+FAM 
Sbjct: 209 NSYFFLNVHPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMT 268

Query: 253 RLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVGTPARPGWVLP 312
           +LGYP +R+ I+ETGWPN GD D+ GANI NAA YNRN++K+++A PP+GTP+RPG  +P
Sbjct: 269 KLGYPHMRLAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIP 328

Query: 313 TIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPLPAAETNEPYKGK 372
           T +FSL+NENQK G GT+RH+G+L+P+ S +Y++D +G+TP   F PLP    N PYKG+
Sbjct: 329 TFVFSLFNENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQ 388

Query: 373 IWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHASFAFSSYWAQFR 432
           +WC+   GAN++E+   L  AC Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR
Sbjct: 389 VWCVPVEGANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFR 448

Query: 433 KVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
                C+FNGLA +T  +P   +CKFPSVTL
Sbjct: 449 NQSIQCFFNGLAHETTTNPGNDRCKFPSVTL 478

BLAST of Cp4.1LG05g00510 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.1e-100
Identity = 189/464 (40.73%), Postives = 287/464 (61.85%), Query Frame = 0

Query: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60
           MA+ F    L   FLS   A  S +VG+NYG++ NNLP P   V+L+KS     +KI+D 
Sbjct: 1   MAVSFLPYFLILSFLSAIDAH-SGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDT 60

Query: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120
           +  +L +L NS IKV V +PN+L+ + +S+Q+ AD+W++T+I+P++P  +I  + VGNE+
Sbjct: 61  DKNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEV 120

Query: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHK-VKVGTSSAMDVLQSSFPPSNGTFRADISD 180
                   +  LV AM+ I  SL  Y + K +K+ +  A+  L +S+PPS+G+F+ ++ +
Sbjct: 121 FVDPTITPY--LVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIE 180

Query: 181 RVMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNL 240
            V+KPML  L +T S+  ++ YPFF +A++   I LDYALF+ +N    D G+GL Y +L
Sbjct: 181 PVVKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFK-ENAGNIDSGTGLKYNSL 240

Query: 241 FDQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRV-TAK 300
           FD  +D+V  A+  +G+  +++ + ETGWP+ GD ++IGA+  NAA YN  +VKRV T K
Sbjct: 241 FDAQIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGK 300

Query: 301 PPVGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFK 360
              GTP RP   L   +F+L+NENQKPG  +ER++GL YPN   VY +  + K+      
Sbjct: 301 ---GTPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKKST----T 360

Query: 361 PLPAAETNEP--YKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSL 420
           P+       P  ++G  WC+ +    K ++  AL +AC +G   C PIQ G  CY P+SL
Sbjct: 361 PVNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESL 420

Query: 421 IRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
             HAS+AF+SY+ +  +  GTC+F G A    + P YGKC+FP+
Sbjct: 421 EAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPT 453

BLAST of Cp4.1LG05g00510 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 353.6 bits (906), Expect = 2.4e-97
Identity = 182/468 (38.89%), Postives = 276/468 (58.97%), Query Frame = 0

Query: 8   LLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDVNPQILKS 67
           L+     LS  S   +  +G+NYG++ + LP     V L+KS     VKI+D +P +LK+
Sbjct: 7   LIFLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKA 66

Query: 68  LNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEIISSTGNQ 127
           L+ S IKV+V +PN+L+ + +   + A  WV+ N+  ++P  +I  + VGNE+   T N 
Sbjct: 67  LSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNT 126

Query: 128 TWISLVPAMRRIKRSLKIYGIHK-VKVGTSSAMDVLQSSFPPSNGTFRADISDRVMKPML 187
           T   L+PAMR I ++L  + +H  +K+ +  A+  LQ+S+P S+G+FR ++ D V+KPML
Sbjct: 127 TSF-LIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPML 186

Query: 188 QFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLFDQMVDS 247
            FL  T S   ++VYPFF +  +   I LDYAL   +N    D G+GL Y NLFD  +D+
Sbjct: 187 DFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLR-ENPGMVDSGNGLRYFNLFDAQIDA 246

Query: 248 VIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPPVGTPAR 307
           V  AM  L Y DI I + ETGWP+ GD +++GA + NAA YN N+++R+  +   GTP R
Sbjct: 247 VFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRG--GTPLR 306

Query: 308 PGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLS----------GKTP--- 367
           P   L   +F+L+NEN+K G  +ER++GL +P+   VY++  +          G TP   
Sbjct: 307 PKADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTPVTG 366

Query: 368 -EADFKPLPAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYE 427
            +   KP  +   ++   G  WC+ +  A +  + G L +AC +G   C PIQ G  CY 
Sbjct: 367 GDQVTKPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYS 426

Query: 428 PDSLIRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           PD+L  HASFAF+SY+ +  + GG+CYF G A    + P YG+C+FP+
Sbjct: 427 PDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPT 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q069154.8e-15154.10Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FJU97.9e-9338.43Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q8VYE59.0e-8935.91Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9M0698.4e-8737.07Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G864.6e-8537.47Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023532592.10.0100.00probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo][more]
XP_022931078.10.099.57probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata][more]
XP_022996249.10.098.71probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita maxima][more]
KAG6606297.10.099.14putative glucan endo-1,3-beta-glucosidase A6, partial [Cucurbita argyrosperma su... [more]
KAG7036237.10.099.33putative glucan endo-1,3-beta-glucosidase A6 [Cucurbita argyrosperma subsp. argy... [more]
Match NameE-valueIdentityDescription
A0A6J1EXC80.099.57(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 P... [more]
A0A6J1K8750.098.71(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=... [more]
A0A5A7VIC45.77e-30286.67(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3CCE35.77e-30286.67(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV... [more]
A0A0A0LBR18.52e-30286.85(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3... [more]
Match NameE-valueIdentityDescription
AT3G07320.19.3e-19068.33O-Glycosyl hydrolases family 17 protein [more]
AT3G23770.15.7e-15556.35O-Glycosyl hydrolases family 17 protein [more]
AT4G14080.13.4e-15254.10O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.13.1e-10040.73O-Glycosyl hydrolases family 17 protein [more]
AT2G05790.12.4e-9738.89O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 373..458
e-value: 1.7E-37
score: 140.6
IPR012946X8 domainPFAMPF07983X8coord: 374..445
e-value: 7.3E-17
score: 61.8
NoneNo IPR availableGENE3D1.20.58.1040coord: 369..462
e-value: 3.7E-6
score: 29.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..355
e-value: 1.0E-116
score: 391.4
NoneNo IPR availablePANTHERPTHR32227:SF341BNAC03G35190D PROTEINcoord: 13..456
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 26..347
e-value: 5.8E-104
score: 348.0
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 259..272
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 13..456
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g00510.1Cp4.1LG05g00510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds