Cp4.1LG05g00460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g00460
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMethyltransferase
LocationCp4.1LG05: 741232 .. 746041 (+)
RNA-Seq ExpressionCp4.1LG05g00460
SyntenyCp4.1LG05g00460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAACTCTATTGACTTCAATTCCTTTTTTATTCTAGCAACCAACAAAATAAGAAACTGTATGGACAATAGGCATTTAGAAAAATAAAACATAAAGTAAATAGATAAAATAGATAAAGAAATAGAGTTATTTTGTCTCTCTATAGCCTGCAGTTTTTGCTATTTCATCATGAATTAGCTATTAATCAATCTCTACATACTATATACTATAGTTTTACCTATATAAACATTCTTAAAAAATGGGCATGAGGGACCGTAAATTTTACGGCGTATCTATCCTACTGTAAGAACTATTTTATTATTATTTGTGTAGGTACCCATACATCCTTAATTCGACCTCCACTGCCAGAGCTTAAATAACAATGAAAATCAATGGGAAGATAGATTTAAAGAAGTGAATGAAGATACAGACACCCCGTTGAGAGGAAGGAAGGGACGTGGAAGTGGGGACGGGCAAGGAGCTGACTTATTTAAAGCTTCACCTCCATGGACAGGGAGAAACACACACACACACACACACTCCTCTCTCTCATTCGCTGGTACAGTCCTCTCTTTCTCTGGGGACAGTGAAACCAGAGAGAGATTTGGGCCTGTTCTGTTCGCTCCCAGGTTTGGGTGAAGGCTTAACACACTCAAAAGGTGAACTCTTTCTTTCAGTTTCAGGTTAAAGCTCAGATCTCTCTCTTGTTTCCCTTTCTCTGGACAGCATTCTGTCTATTCTCTTCTTAAAACCTTCGTTTCATCTTGTTCTTTCTCTAAGTTTTGTCACATTTATCTCTTTGAAGCTCTCTAGTGTGTCCTATAAAATGGCCCTTCTATTCTATTTTCTTCCACAACAGATCTGACCATCGACGCTTCCTTCACCGTTCTTTTCTATTCTTCCTATTTTTAGATCTTTTTTTTTTTTTTACCCGTGTGTCTTAATTTTGACTATGTTTTTTTAACTCTTCTGGGTATTTTCCTCTTTTTTAACATTGTTGATTGATCTTGGGGGAATTACAGATGCTTTCTCTCGTCCATTTCTTTGGGTTTTGTTCTTTTTGAGCAAATACTTGGCTGATTTGCTTCATGTTCTGAGAAGCTCTAGTTCCTTTTATNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAATCACCATCTTGACATTGGCAAATCAAAGGGGAAGGGGGGAGGCAAAACGCTGAGGATTGAATTTCATTTGGTTTTCAATTTCTCTTATTGATACTTGGATTCAAGGGGTGGGTTTCCTTTGTGTGTTTGGGGGCCCAACACCATGTGTGAAAATTCCAATCAAGGGGGGCAAAAGAAAGTGGAAATTGTCGGTTGTTGTTGTCGGATGTTAATTTTACAGCTGCACTTTACATTCTTTCATGTTCTTCATTTTACAATTTGATTTCCTTAGCTTTTTTCTTTAATGTTCCCTTATCTGAAGATATGGTTTCAAGATTAAACTAACTGGATCTATTGTTTTCTCCATATTTTCATTTTGTAACTTGTAAGGGTAGCTTTTGCTTTCTGTTGAGTGTTTTAGCTGTTTGTAGATTCAATGGATTTGTTTCAGGCTTCAGTTTTTTGGGTGCATTTTCTAGCAAGGAAGTCGAAATTCTGTTTAGTTTCTGTTCTTAAGAATATGTTAAACATTTCAAAGCTAATCAATAGATGTAAGCATTAATATAACTCAGATAACAAATAATGAATGCATGATATAGACAGTGACTAATATTCTGGCCCCTTGGATTTTTTGCAGGGTGTTGGTTGTTAATAGGGAATCATGAAGTACAAAGATGGGAAGCTAGGACATAAGCAAGACAAGAATGCAAGAGTCCTCCCTATGACAATTCTGTTGTTTGTGTTATGTGGATTCTCATTCTATCTAGGTGGAATCTTTTGTTCTGAGAAAGAGGGAGTCAATGTCAATACAGCCATGGACATTGAAAACTTAGTCACCTCATCGAGGGACACGGTCGTGTCCCCTCTCCAACTTAAACCTGTTGTCTTTCAGGAATGCAGCAAGGAGTACCAAGATTACACTCCTTGCACAGATCCAAGGGTATATCCCTTTTTAACATTTTTGGCGCTTCCATAATTTTCATATCTAGAAATATCAGTAATCTGATTTGCACTTGTGAGTGCAGAAATGGAAGAAGTATGGTTTTTATCGGCTTACTTTCATGGAACGACATTGTCCACCCATGTTTGAAAGAAAGGAATGCTTAATTCCACCACCAGATGGGTATAAACCACCAATTAGATGGCCAAAAAGCAAGGATGAGTGCTGGTACAGGTAATATGTTCTTTTTTCTCATGAAGTTCTTTTTGCAAAGGACATCTTCTCCCTTATATAGATTTCGAACTTCCATCTCTTCTAAGTTTAACTAATTTCATGTTAACTGGACTTTAGGAACGTACCATATGATTGGATCAATAAACAGAAATCTAATCAAAATTGGCTGAGGAAAGAAGGTGAAAAGTTCCTCTTTCCTGGTGGGGGAACCATGTTTCCAAATGGTGTTAGTGCATACGTCGATCTGATGCAAGATCTTATACCAGAAATGAAGGATGGAACTATTCGAACAGCCATTGATACTGGCTGCGGGGTAGGTTCTACTTTCAATTCCATCTTGTGGTTTCTTATCATCTTTGCATTTAGAGGAAACTTTAACTGTTTACTTCAAAAAAAAATTCTAGCTTCAATTGATTATTTTCCATGGTCACCTAAGGATCATATTCTAAATTTTATCAAAGAGAGGCACAAAAAGTTTTGAACCCTAAGATGAATCACATTCTCTAGGTTGCAAGTTGGGGAGGAGATCTACTAGACCGTGGAATTTTAACAGTTTCTCTTGCACCAAGAGATAACCATGAAGCTCAAGTCCAATTTGCATTGGAACGTGGAATTCCAGCAATACTTGGCATCATATCGACACAACGACTTCCATTCCCATCAAGCTCCTTTGATATGGCTCATTGCTCAAGATGCCTTATCCCATGGACTGAGTTTGGTAAATTTCTATTTTTCAAAAAATTGAATCATGCCTAATTTCGTACAAGAACAAAGAGTTATTGCCTAACTGGGTTTTACCTATCATTAGAAACACATTGAACATAGATTATGAGAATGTTTTACCACTATATTCAATCGAGTAATTGAATAACTCGATTGTTATGTTTTTCTCAAATTTCTGATTATCACACTCTGTTTTCAGGTGGGATTTACTTGCTAGAAATCAACAGAATCCTTCGTCCTGGAGGCCTTTGGGTCCTATCTGGTCCCCCAGTCAACTATGAAAACCGTTGGAGAGGATGGAATACAACTGTAGAAGAACAAAGATCAGACTATGAAAAATTGCAGGAACTCCTAACTTCAATGTGCTTTACCCTTTACAACAAAAAAGATGATATTGCCGTTTGGCAGAAATCTACTGATCCTAATTGCTTCGACAAGATTGCTGCTGATGCTTACCCTCCTAAGTGTGACGACAGCCTTGAACCAGATTCAGCATGGTACAGTCCATTGCGCACTTGTGTCATTTCTCCCGACCCAAAACTGAAGAGAACAAGTTTGATGGCTATCCCCAAATGGCCAGATCGTTTGCACACCTCACCCGAACGAGTTTCAGATGTTTATGGTGGCAGTTCTGGCACTTTCAAGCACGATGATAGTAAATGGATGGTCCGAGCCAAACATTACAAGAAATTGCTTCCTGCAATTGGGACTGAAAAGATCAGAAATGTTATGGATATGAATACAGTTTATGGAGGTTTTGCTGCTGCTATTATTGATGATCCCTTGTGGGTTATGAATGTGGTTTCTTCCTACGCTGCTAATACTCTTCCTGTGGTCTATGATCGTGGCCTTATTGGAACTTATCATGACTGGTAAAACCAAAGCAACACTTCTTTTCCTTCATACTTTTTGGTTGGTCTTCATAGACTTACACGAATGAAACTTTTGGTTACCTTGCAATGGCTAATTTGGCAGGTGTGAAGCATTTTCAACATATCCTCGGACTTATGACCTGCTCCATCTTGATGGGCTTTTCACTGCAGAAGGCCATAGGTAATAAAAGTTGATGTGCTTTGAAATCTTTTTCGTTAAATGGATGATTGTAAAGGTAGTCCAATTAAGGGAGTTTCTAATCATTTGGAATTCCTGGCAGATGTGAGATGAAGTATGTGCTTTTGGAGATGGATCGAATCCTGCGTCCTAATGGGTATGCGATAATTCGTGAGTCAAGCTACTACGTAGATGCAGTAGCCTCAATGGCTAAAGGGATGAGATGGGGATGTCGTAAAGAAGAAACCGAATACAGTACTGAAAAAGAGAAGATGTTAATTTGCCATAAAAAACTCTGGTATTCTTCTAGCCGGAAATCAAGATGACAGAAGGAGTAATAGAAGCTGACCAAATCGAGGTACTTACGCTTAGAGAATCAAAGAGAAGAAAGTTCAATAGAAGCACAAAGAGATTAGTTTATTCTTACAAAATACTGGGAAAAGTACGATCTTTCGCCTCTTCGTCGATTGAAGAATCGGGTACCAAATAATGTGGTTATATTTATTTTACCGGGCCTCTTTCATATAGCTATTTTCTTTAGTAAGGGGGCAAATTGTGCGTCATTTTCCAACTTTTCCTCTGCATCTTTTTCTGGCCAATACTACTTTTTGTCGTCTAGTAATTCATGGGCTGCTGAGCTTGAAGGGTTCAAACCGCAAGCCCATTCTTGTGATTTGGCTTTTAATTATTGTTTTTGTTTTTGTTATTTTTAAATATAATCATTTCCTACTGTATAGCCGAA

mRNA sequence

AAAAAACTCTATTGACTTCAATTCCTTTTTTATTCTAGCAACCAACAAAATAAGAAACTGTATGGACAATAGGCATTTAGAAAAATAAAACATAAAGTAAATAGATAAAATAGATAAAGAAATAGAGTTATTTTGTCTCTCTATAGCCTGCAGTTTTTGCTATTTCATCATGAATTAGCTATTAATCAATCTCTACATACTATATACTATAGTTTTACCTATATAAACATTCTTAAAAAATGGGCATGAGGGACCGTAAATTTTACGGCGTATCTATCCTACTGTAAGAACTATTTTATTATTATTTGTGTAGGTACCCATACATCCTTAATTCGACCTCCACTGCCAGAGCTTAAATAACAATGAAAATCAATGGGAAGATAGATTTAAAGAAGTGAATGAAGATACAGACACCCCGTTGAGAGGAAGGAAGGGACGTGGAAGTGGGGACGGGCAAGGAGCTGACTTATTTAAAGCTTCACCTCCATGGACAGGGAGAAACACACACACACACACACACTCCTCTCTCTCATTCGCTGGTACAGTCCTCTCTTTCTCTGGGGACAGTGAAACCAGAGAGAGATTTGGGCCTGTTCTGTTCGCTCCCAGGTTTGGGTGAAGGCTTAACACACTCAAAAGGGTGTTGGTTGTTAATAGGGAATCATGAAGTACAAAGATGGGAAGCTAGGACATAAGCAAGACAAGAATGCAAGAGTCCTCCCTATGACAATTCTGTTGTTTGTGTTATGTGGATTCTCATTCTATCTAGGTGGAATCTTTTGTTCTGAGAAAGAGGGAGTCAATGTCAATACAGCCATGGACATTGAAAACTTAGTCACCTCATCGAGGGACACGGTCGTGTCCCCTCTCCAACTTAAACCTGTTGTCTTTCAGGAATGCAGCAAGGAGTACCAAGATTACACTCCTTGCACAGATCCAAGGAAATGGAAGAAGTATGGTTTTTATCGGCTTACTTTCATGGAACGACATTGTCCACCCATGTTTGAAAGAAAGGAATGCTTAATTCCACCACCAGATGGGTATAAACCACCAATTAGATGGCCAAAAAGCAAGGATGAGTGCTGGTACAGAATCCTTCGTCCTGGAGGCCTTTGGGTCCTATCTGGTCCCCCAGTCAACTATGAAAACCGTTGGAGAGGATGGAATACAACTGTAGAAGAACAAAGATCAGACTATGAAAAATTGCAGGAACTCCTAACTTCAATGTGCTTTACCCTTTACAACAAAAAAGATGATATTGCCGTTTGGCAGAAATCTACTGATCCTAATTGCTTCGACAAGATTGCTGCTGATGCTTACCCTCCTAAGTGTGACGACAGCCTTGAACCAGATTCAGCATGGTGTGAAGCATTTTCAACATATCCTCGGACTTATGACCTGCTCCATCTTGATGGGCTTTTCACTGCAGAAGGCCATAGATGTGAGATGAAGTATGTGCTTTTGGAGATGGATCGAATCCTGCGTCCTAATGGGTATGCGATAATTCGTGAGTCAAGCTACTACGTAGATGCAGTAGCCTCAATGGCTAAAGGGATGAGATGGGGATGTCGTAAAGAAGAAACCGAATACAGTACTGAAAAAGAGAAGATGTTAATTTGCCATAAAAAACTCTGGTATTCTTCTAGCCGGAAATCAAGATGACAGAAGGAGTAATAGAAGCTGACCAAATCGAGGTACTTACGCTTAGAGAATCAAAGAGAAGAAAGTTCAATAGAAGCACAAAGAGATTAGTTTATTCTTACAAAATACTGGGAAAAGTACGATCTTTCGCCTCTTCGTCGATTGAAGAATCGGGTACCAAATAATGTGGTTATATTTATTTTACCGGGCCTCTTTCATATAGCTATTTTCTTTAGTAAGGGGGCAAATTGTGCGTCATTTTCCAACTTTTCCTCTGCATCTTTTTCTGGCCAATACTACTTTTTGTCGTCTAGTAATTCATGGGCTGCTGAGCTTGAAGGGTTCAAACCGCAAGCCCATTCTTGTGATTTGGCTTTTAATTATTGTTTTTGTTTTTGTTATTTTTAAATATAATCATTTCCTACTGTATAGCCGAA

Coding sequence (CDS)

ATGAAGTACAAAGATGGGAAGCTAGGACATAAGCAAGACAAGAATGCAAGAGTCCTCCCTATGACAATTCTGTTGTTTGTGTTATGTGGATTCTCATTCTATCTAGGTGGAATCTTTTGTTCTGAGAAAGAGGGAGTCAATGTCAATACAGCCATGGACATTGAAAACTTAGTCACCTCATCGAGGGACACGGTCGTGTCCCCTCTCCAACTTAAACCTGTTGTCTTTCAGGAATGCAGCAAGGAGTACCAAGATTACACTCCTTGCACAGATCCAAGGAAATGGAAGAAGTATGGTTTTTATCGGCTTACTTTCATGGAACGACATTGTCCACCCATGTTTGAAAGAAAGGAATGCTTAATTCCACCACCAGATGGGTATAAACCACCAATTAGATGGCCAAAAAGCAAGGATGAGTGCTGGTACAGAATCCTTCGTCCTGGAGGCCTTTGGGTCCTATCTGGTCCCCCAGTCAACTATGAAAACCGTTGGAGAGGATGGAATACAACTGTAGAAGAACAAAGATCAGACTATGAAAAATTGCAGGAACTCCTAACTTCAATGTGCTTTACCCTTTACAACAAAAAAGATGATATTGCCGTTTGGCAGAAATCTACTGATCCTAATTGCTTCGACAAGATTGCTGCTGATGCTTACCCTCCTAAGTGTGACGACAGCCTTGAACCAGATTCAGCATGGTGTGAAGCATTTTCAACATATCCTCGGACTTATGACCTGCTCCATCTTGATGGGCTTTTCACTGCAGAAGGCCATAGATGTGAGATGAAGTATGTGCTTTTGGAGATGGATCGAATCCTGCGTCCTAATGGGTATGCGATAATTCGTGAGTCAAGCTACTACGTAGATGCAGTAGCCTCAATGGCTAAAGGGATGAGATGGGGATGTCGTAAAGAAGAAACCGAATACAGTACTGAAAAAGAGAAGATGTTAATTTGCCATAAAAAACTCTGGTATTCTTCTAGCCGGAAATCAAGATGA

Protein sequence

MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTSSRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECLIPPPDGYKPPIRWPKSKDECWYRILRPGGLWVLSGPPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIAADAYPPKCDDSLEPDSAWCEAFSTYPRTYDLLHLDGLFTAEGHRCEMKYVLLEMDRILRPNGYAIIRESSYYVDAVASMAKGMRWGCRKEETEYSTEKEKMLICHKKLWYSSSRKSR
Homology
BLAST of Cp4.1LG05g00460 vs. ExPASy Swiss-Prot
Match: Q94II3 (Probable methyltransferase PMT21 OS=Arabidopsis thaliana OX=3702 GN=ERD3 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.6e-114
Identity = 239/603 (39.64%), Postives = 281/603 (46.60%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKD K   K +K +R+LP T+LL +LCG SFYLGG++C    G N+    D+    +S
Sbjct: 1   MKYKDEKY-EKAEKGSRILPKTVLLILLCGLSFYLGGLYC----GKNIIEVSDVAKAESS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           S D V   LQ+K V F ECS +YQDYTPCTDPRKWKKYG +RLTFMERHCPP+F+RK+CL
Sbjct: 61  SLD-VDDSLQVKSVSFSECSSDYQDYTPCTDPRKWKKYGTHRLTFMERHCPPVFDRKQCL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWY-------------------------------------- 180
           +PPPDGYKPPIRWPKSKDECWY                                      
Sbjct: 121 VPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFIFPGGGTMFPHGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 -----------------------------------------------RILRPGGLWVLSG 300
                                                          RILRPGG WVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSNSFDMAHCSRCLIPWTEFGGVYLLEVHRILRPGGFWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRW+GW+TT+EEQRS+YEKLQELL+SMCF +Y KKDDIAVWQKS D  C++K++
Sbjct: 301 PPVNYENRWKGWDTTIEEQRSNYEKLQELLSSMCFKMYAKKDDIAVWQKSPDNLCYNKLS 360

BLAST of Cp4.1LG05g00460 vs. ExPASy Swiss-Prot
Match: Q9C6S7 (Probable methyltransferase PMT20 OS=Arabidopsis thaliana OX=3702 GN=At1g31850 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.3e-109
Identity = 222/605 (36.69%), Postives = 275/605 (45.45%), Query Frame = 0

Query: 4   KDGKLGHKQDK-NARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVT--- 63
           K GK   + +K  +R+L +T+L    CGFSFYLGGIFCSE++ +    A D+    T   
Sbjct: 2   KSGKQSSQPEKGTSRILSLTVLFIAFCGFSFYLGGIFCSERDKI---VAKDVTRTTTKAV 61

Query: 64  -SSRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKE 123
            S ++   +P+Q+K V F EC  E+QDYTPCTDP++WKKYG +RL+F+ERHCPP++E+ E
Sbjct: 62  ASPKEPTATPIQIKSVSFPECGSEFQDYTPCTDPKRWKKYGVHRLSFLERHCPPVYEKNE 121

Query: 124 CLIPPPDGYKPPIRWPKSKDECWY------------------------------------ 183
           CLIPPPDGYKPPIRWPKS+++CWY                                    
Sbjct: 122 CLIPPPDGYKPPIRWPKSREQCWYRNVPYDWINKQKSNQHWLKKEGDKFHFPGGGTMFPR 181

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 182 GVSHYVDLMQDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILSLSLAPRDNHEAQVQF 241

Query: 244 -------------------------------------------------RILRPGGLWVL 303
                                                            RI+RPGG WVL
Sbjct: 242 ALERGIPAILGIISTQRLPFPSNAFDMAHCSRCLIPWTEFGGIYLLEIHRIVRPGGFWVL 301

Query: 304 SGPPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDK 332
           SGPPVNY  RWRGWNTT+E+Q+SDY KLQ LLTSMCF  Y +KDDIAVWQK +D +C+DK
Sbjct: 302 SGPPVNYNRRWRGWNTTMEDQKSDYNKLQSLLTSMCFKKYAQKDDIAVWQKLSDKSCYDK 361

BLAST of Cp4.1LG05g00460 vs. ExPASy Swiss-Prot
Match: Q9SZX8 (Probable methyltransferase PMT17 OS=Arabidopsis thaliana OX=3702 GN=At4g10440 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.3e-28
Identity = 108/524 (20.61%), Postives = 159/524 (30.34%), Query Frame = 0

Query: 76  FQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECLIPPPDGYKPPIRWPK 135
           F+ C     +YTPC D ++ +++    + + ERHCP   E   CLIPPP  YK P +WP+
Sbjct: 93  FEPCELSLSEYTPCEDRQRGRRFDRNMMKYRERHCPVKDELLYCLIPPPPNYKIPFKWPQ 152

Query: 136 SKDECWY----------------------------------------------------- 195
           S+D  WY                                                     
Sbjct: 153 SRDYAWYDNIPHKELSVEKAVQNWIQVEGDRFRFPGGGTMFPRGADAYIDDIARLIPLTD 212

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 213 GGIRTAIDTGCGVASFGAYLLKRDIMAVSFAPRDTHEAQVQFALERGVPAIIGIMGSRRL 272

Query: 256 -------------------------------RILRPGGLWVLSGPPVNYENRWRGWNTTV 315
                                          R+LRPGG W+LSGPP+N++  WRGW  T 
Sbjct: 273 PYPARAFDLAHCSRCLIPWFKNDGLYLMEVDRVLRPGGYWILSGPPINWKQYWRGWERTE 332

Query: 316 EEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDP-NCFDKIAADAYPPKC------- 325
           E+ + + + ++++  S+C+    +K D+++WQK  +   C      +  PP C       
Sbjct: 333 EDLKKEQDSIEDVAKSLCWKKVTEKGDLSIWQKPLNHIECKKLKQNNKSPPICSSDNADS 392

BLAST of Cp4.1LG05g00460 vs. ExPASy Swiss-Prot
Match: Q9C884 (Probable methyltransferase PMT18 OS=Arabidopsis thaliana OX=3702 GN=At1g33170 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.0e-27
Identity = 107/529 (20.23%), Postives = 155/529 (29.30%), Query Frame = 0

Query: 76  FQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECLIPPPDGYKPPIRWPK 135
           F+ C     +YTPC D  + +++    + + ERHCP   E   CLIPPP  YK P +WP+
Sbjct: 110 FEPCDMSLSEYTPCEDRERGRRFDRNMMKYRERHCPSKDELLYCLIPPPPNYKIPFKWPQ 169

Query: 136 SKDECWY----------------------------------------------------- 195
           S+D  WY                                                     
Sbjct: 170 SRDYAWYDNIPHKELSIEKAIQNWIQVEGERFRFPGGGTMFPRGADAYIDDIARLIPLTD 229

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 230 GAIRTAIDTGCGVASFGAYLLKRDIVAMSFAPRDTHEAQVQFALERGVPAIIGIMGSRRL 289

Query: 256 -------------------------------RILRPGGLWVLSGPPVNYENRWRGWNTTV 315
                                          R+LRPGG W+LSGPP+N++  W+GW  + 
Sbjct: 290 PYPARAFDLAHCSRCLIPWFQNDGLYLTEVDRVLRPGGYWILSGPPINWKKYWKGWERSQ 349

Query: 316 EEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQK--------------STDPNC------- 329
           E+ + + + +++   S+C+    +K D+++WQK               T P C       
Sbjct: 350 EDLKQEQDSIEDAARSLCWKKVTEKGDLSIWQKPINHVECNKLKRVHKTPPLCSKSDLPD 409

BLAST of Cp4.1LG05g00460 vs. ExPASy Swiss-Prot
Match: O80844 (Probable methyltransferase PMT16 OS=Arabidopsis thaliana OX=3702 GN=At2g45750 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.0e-25
Identity = 106/537 (19.74%), Postives = 162/537 (30.17%), Query Frame = 0

Query: 68  PLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECLIPPPDGY 127
           P+    V F  C+    ++TPC D ++  K+   RL + +RHCP   E  +C IP P GY
Sbjct: 79  PVTETAVSFPSCAAALSEHTPCEDAKRSLKFSRERLEYRQRHCPEREEILKCRIPAPYGY 138

Query: 128 KPPIRWPKSKDECWY--------------------------------------------- 187
           K P RWP S+D  W+                                             
Sbjct: 139 KTPFRWPASRDVAWFANVPHTELTVEKKNQNWVRYENDRFWFPGGGTMFPRGADAYIDDI 198

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 199 GRLIDLSDGSIRTAIDTGCGVASFGAYLLSRNITTMSFAPRDTHEAQVQFALERGVPAMI 258

Query: 248 ---------------------------------------RILRPGGLWVLSGPPVNYENR 307
                                                  R+LRPGG W+LSGPP+N++ R
Sbjct: 259 GIMATIRLPYPSRAFDLAHCSRCLIPWGQNDGAYLMEVDRVLRPGGYWILSGPPINWQKR 318

Query: 308 WRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQK------------------- 325
           W+GW  T+++  ++  +++++  S+C+    ++DD+A+WQK                   
Sbjct: 319 WKGWERTMDDLNAEQTQIEQVARSLCWKKVVQRDDLAIWQKPFNHIDCKKTREVLKNPEF 378

BLAST of Cp4.1LG05g00460 vs. NCBI nr
Match: XP_023532722.1 (probable methyltransferase PMT21 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532723.1 probable methyltransferase PMT21 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532724.1 probable methyltransferase PMT21 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532725.1 probable methyltransferase PMT21 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 603 bits (1556), Expect = 1.76e-212
Identity = 332/602 (55.15%), Postives = 332/602 (55.15%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS
Sbjct: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL
Sbjct: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGGLWVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 360

BLAST of Cp4.1LG05g00460 vs. NCBI nr
Match: KAG7036230.1 (putative methyltransferase PMT21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600 bits (1546), Expect = 5.82e-211
Identity = 330/602 (54.82%), Postives = 331/602 (54.98%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIEN VTS
Sbjct: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENSVTS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPP+FERKECL
Sbjct: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGGLWVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 360

BLAST of Cp4.1LG05g00460 vs. NCBI nr
Match: XP_022931156.1 (probable methyltransferase PMT21 [Cucurbita moschata] >XP_022931157.1 probable methyltransferase PMT21 [Cucurbita moschata] >XP_022931158.1 probable methyltransferase PMT21 [Cucurbita moschata] >XP_022931159.1 probable methyltransferase PMT21 [Cucurbita moschata])

HSP 1 Score: 598 bits (1542), Expect = 2.36e-210
Identity = 329/602 (54.65%), Postives = 330/602 (54.82%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIEN VTS
Sbjct: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENSVTS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           SRDTV SPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPP+FERKECL
Sbjct: 61  SRDTVASPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGGLWVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 360

BLAST of Cp4.1LG05g00460 vs. NCBI nr
Match: XP_022995627.1 (probable methyltransferase PMT21 isoform X1 [Cucurbita maxima] >XP_022995628.1 probable methyltransferase PMT21 isoform X1 [Cucurbita maxima] >XP_022995629.1 probable methyltransferase PMT21 isoform X1 [Cucurbita maxima] >XP_022995630.1 probable methyltransferase PMT21 isoform X1 [Cucurbita maxima])

HSP 1 Score: 595 bits (1533), Expect = 5.49e-209
Identity = 327/602 (54.32%), Postives = 329/602 (54.65%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           M Y+DGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVT 
Sbjct: 1   MMYRDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTP 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPP+FERKECL
Sbjct: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGGLWVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 360

BLAST of Cp4.1LG05g00460 vs. NCBI nr
Match: KAG6606289.1 (putative methyltransferase PMT21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 556 bits (1434), Expect = 2.98e-194
Identity = 310/582 (53.26%), Postives = 311/582 (53.44%), Query Frame = 0

Query: 21  MTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTSSRDTVVSPLQLKPVVFQECS 80
           MTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIEN VTSSRDTVVSPLQLKPVVFQECS
Sbjct: 1   MTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENSVTSSRDTVVSPLQLKPVVFQECS 60

Query: 81  KEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECLIPPPDGYKPPIRWPKSKDEC 140
           KEYQDYTPCTDPRKWKKYGFYRLTFMERHCPP+FERKECLIPPPDGYKPPIRWPKSKDEC
Sbjct: 61  KEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPVFERKECLIPPPDGYKPPIRWPKSKDEC 120

Query: 141 WYR--------------------------------------------------------- 200
           WYR                                                         
Sbjct: 121 WYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGVSAYVDLMQDLIPEMKDGTIR 180

Query: 201 ------------------------------------------------------------ 260
                                                                       
Sbjct: 181 TAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFALERGIPAILGIISTQRLPFPS 240

Query: 261 ----------------------------ILRPGGLWVLSGPPVNYENRWRGWNTTVEEQR 320
                                       ILRPGGLWVLSGPPVNYENRWRGWNTTVEEQR
Sbjct: 241 SSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSGPPVNYENRWRGWNTTVEEQR 300

Query: 321 SDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIAADAYPPKCDDSLEPDSAW-- 332
           SDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIAADAYPPKCDDSLEPDSAW  
Sbjct: 301 SDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIAADAYPPKCDDSLEPDSAWYS 360

BLAST of Cp4.1LG05g00460 vs. ExPASy TrEMBL
Match: A0A6J1ETJ5 (Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111437422 PE=3 SV=1)

HSP 1 Score: 598 bits (1542), Expect = 1.14e-210
Identity = 329/602 (54.65%), Postives = 330/602 (54.82%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIEN VTS
Sbjct: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENSVTS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           SRDTV SPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPP+FERKECL
Sbjct: 61  SRDTVASPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGGLWVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 360

BLAST of Cp4.1LG05g00460 vs. ExPASy TrEMBL
Match: A0A6J1JZG3 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491107 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 2.66e-209
Identity = 327/602 (54.32%), Postives = 329/602 (54.65%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           M Y+DGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVT 
Sbjct: 1   MMYRDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTP 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPP+FERKECL
Sbjct: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPNGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGGLWVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGLWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 360

BLAST of Cp4.1LG05g00460 vs. ExPASy TrEMBL
Match: A0A0A0LE72 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G636940 PE=3 SV=1)

HSP 1 Score: 555 bits (1430), Expect = 1.15e-193
Identity = 305/602 (50.66%), Postives = 317/602 (52.66%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGK G+  DKNARVLPMTILLFVLCGFSFYLGGIFCSEKEG NVNT+MD+ + V S
Sbjct: 1   MKYKDGKPGYNPDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGFNVNTSMDVGDSVAS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           +RDT VSPLQLKPV FQECS +YQDYTPCTDPRKWKKYG +RLTFMERHCPP+FERKECL
Sbjct: 61  ARDTAVSPLQLKPVTFQECSSDYQDYTPCTDPRKWKKYGLHRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPHGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMTDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGG WVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGFWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKS+DPNCF+KIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSSDPNCFNKIA 360

BLAST of Cp4.1LG05g00460 vs. ExPASy TrEMBL
Match: A0A1S3CCF8 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499239 PE=3 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 3.27e-193
Identity = 304/602 (50.50%), Postives = 317/602 (52.66%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGK G+  DKNARVLPMTILLFVLCGFSFYLGGIFCSEKEG+NVNT++D+ + V S
Sbjct: 1   MKYKDGKPGYNPDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGLNVNTSLDVGDSVAS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
            RDT VSPLQLKPV FQECS +YQDYTPCTDPRKWKKYG +RLTFMERHCPP+FERKECL
Sbjct: 61  PRDTAVSPLQLKPVTFQECSSDYQDYTPCTDPRKWKKYGLHRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPHGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMTDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGG WVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGFWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKS+DPNCF+KIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSSDPNCFNKIA 360

BLAST of Cp4.1LG05g00460 vs. ExPASy TrEMBL
Match: A0A5D3DBT4 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00010 PE=3 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 3.27e-193
Identity = 304/602 (50.50%), Postives = 317/602 (52.66%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKDGK G+  DKNARVLPMTILLFVLCGFSFYLGGIFCSEKEG+NVNT++D+ + V S
Sbjct: 1   MKYKDGKPGYNPDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGLNVNTSLDVGDSVAS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
            RDT VSPLQLKPV FQECS +YQDYTPCTDPRKWKKYG +RLTFMERHCPP+FERKECL
Sbjct: 61  PRDTAVSPLQLKPVTFQECSSDYQDYTPCTDPRKWKKYGLHRLTFMERHCPPVFERKECL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWYR------------------------------------- 180
           IPPPDGYKPPIRWPKSKDECWYR                                     
Sbjct: 121 IPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFLFPGGGTMFPHGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMTDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 ------------------------------------------------ILRPGGLWVLSG 300
                                                           ILRPGG WVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSSSFDMAHCSRCLIPWTEFGGIYLLEINRILRPGGFWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKS+DPNCF+KIA
Sbjct: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSSDPNCFNKIA 360

BLAST of Cp4.1LG05g00460 vs. TAIR 10
Match: AT4G19120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.8e-115
Identity = 239/603 (39.64%), Postives = 281/603 (46.60%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKD K   K +K +R+LP T+LL +LCG SFYLGG++C    G N+    D+    +S
Sbjct: 1   MKYKDEKY-EKAEKGSRILPKTVLLILLCGLSFYLGGLYC----GKNIIEVSDVAKAESS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           S D V   LQ+K V F ECS +YQDYTPCTDPRKWKKYG +RLTFMERHCPP+F+RK+CL
Sbjct: 61  SLD-VDDSLQVKSVSFSECSSDYQDYTPCTDPRKWKKYGTHRLTFMERHCPPVFDRKQCL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWY-------------------------------------- 180
           +PPPDGYKPPIRWPKSKDECWY                                      
Sbjct: 121 VPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFIFPGGGTMFPHGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 -----------------------------------------------RILRPGGLWVLSG 300
                                                          RILRPGG WVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSNSFDMAHCSRCLIPWTEFGGVYLLEVHRILRPGGFWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRW+GW+TT+EEQRS+YEKLQELL+SMCF +Y KKDDIAVWQKS D  C++K++
Sbjct: 301 PPVNYENRWKGWDTTIEEQRSNYEKLQELLSSMCFKMYAKKDDIAVWQKSPDNLCYNKLS 360

BLAST of Cp4.1LG05g00460 vs. TAIR 10
Match: AT4G19120.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.8e-115
Identity = 239/603 (39.64%), Postives = 281/603 (46.60%), Query Frame = 0

Query: 1   MKYKDGKLGHKQDKNARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVTS 60
           MKYKD K   K +K +R+LP T+LL +LCG SFYLGG++C    G N+    D+    +S
Sbjct: 1   MKYKDEKY-EKAEKGSRILPKTVLLILLCGLSFYLGGLYC----GKNIIEVSDVAKAESS 60

Query: 61  SRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKECL 120
           S D V   LQ+K V F ECS +YQDYTPCTDPRKWKKYG +RLTFMERHCPP+F+RK+CL
Sbjct: 61  SLD-VDDSLQVKSVSFSECSSDYQDYTPCTDPRKWKKYGTHRLTFMERHCPPVFDRKQCL 120

Query: 121 IPPPDGYKPPIRWPKSKDECWY-------------------------------------- 180
           +PPPDGYKPPIRWPKSKDECWY                                      
Sbjct: 121 VPPPDGYKPPIRWPKSKDECWYRNVPYDWINKQKSNQNWLRKEGEKFIFPGGGTMFPHGV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 SAYVDLMQDLIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHEAQVQFAL 240

Query: 241 -----------------------------------------------RILRPGGLWVLSG 300
                                                          RILRPGG WVLSG
Sbjct: 241 ERGIPAILGIISTQRLPFPSNSFDMAHCSRCLIPWTEFGGVYLLEVHRILRPGGFWVLSG 300

Query: 301 PPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDKIA 332
           PPVNYENRW+GW+TT+EEQRS+YEKLQELL+SMCF +Y KKDDIAVWQKS D  C++K++
Sbjct: 301 PPVNYENRWKGWDTTIEEQRSNYEKLQELLSSMCFKMYAKKDDIAVWQKSPDNLCYNKLS 360

BLAST of Cp4.1LG05g00460 vs. TAIR 10
Match: AT1G31850.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.3e-110
Identity = 222/605 (36.69%), Postives = 275/605 (45.45%), Query Frame = 0

Query: 4   KDGKLGHKQDK-NARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVT--- 63
           K GK   + +K  +R+L +T+L    CGFSFYLGGIFCSE++ +    A D+    T   
Sbjct: 2   KSGKQSSQPEKGTSRILSLTVLFIAFCGFSFYLGGIFCSERDKI---VAKDVTRTTTKAV 61

Query: 64  -SSRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKE 123
            S ++   +P+Q+K V F EC  E+QDYTPCTDP++WKKYG +RL+F+ERHCPP++E+ E
Sbjct: 62  ASPKEPTATPIQIKSVSFPECGSEFQDYTPCTDPKRWKKYGVHRLSFLERHCPPVYEKNE 121

Query: 124 CLIPPPDGYKPPIRWPKSKDECWY------------------------------------ 183
           CLIPPPDGYKPPIRWPKS+++CWY                                    
Sbjct: 122 CLIPPPDGYKPPIRWPKSREQCWYRNVPYDWINKQKSNQHWLKKEGDKFHFPGGGTMFPR 181

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 182 GVSHYVDLMQDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILSLSLAPRDNHEAQVQF 241

Query: 244 -------------------------------------------------RILRPGGLWVL 303
                                                            RI+RPGG WVL
Sbjct: 242 ALERGIPAILGIISTQRLPFPSNAFDMAHCSRCLIPWTEFGGIYLLEIHRIVRPGGFWVL 301

Query: 304 SGPPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDK 332
           SGPPVNY  RWRGWNTT+E+Q+SDY KLQ LLTSMCF  Y +KDDIAVWQK +D +C+DK
Sbjct: 302 SGPPVNYNRRWRGWNTTMEDQKSDYNKLQSLLTSMCFKKYAQKDDIAVWQKLSDKSCYDK 361

BLAST of Cp4.1LG05g00460 vs. TAIR 10
Match: AT1G31850.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.3e-110
Identity = 222/605 (36.69%), Postives = 275/605 (45.45%), Query Frame = 0

Query: 4   KDGKLGHKQDK-NARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVT--- 63
           K GK   + +K  +R+L +T+L    CGFSFYLGGIFCSE++ +    A D+    T   
Sbjct: 2   KSGKQSSQPEKGTSRILSLTVLFIAFCGFSFYLGGIFCSERDKI---VAKDVTRTTTKAV 61

Query: 64  -SSRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKE 123
            S ++   +P+Q+K V F EC  E+QDYTPCTDP++WKKYG +RL+F+ERHCPP++E+ E
Sbjct: 62  ASPKEPTATPIQIKSVSFPECGSEFQDYTPCTDPKRWKKYGVHRLSFLERHCPPVYEKNE 121

Query: 124 CLIPPPDGYKPPIRWPKSKDECWY------------------------------------ 183
           CLIPPPDGYKPPIRWPKS+++CWY                                    
Sbjct: 122 CLIPPPDGYKPPIRWPKSREQCWYRNVPYDWINKQKSNQHWLKKEGDKFHFPGGGTMFPR 181

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 182 GVSHYVDLMQDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILSLSLAPRDNHEAQVQF 241

Query: 244 -------------------------------------------------RILRPGGLWVL 303
                                                            RI+RPGG WVL
Sbjct: 242 ALERGIPAILGIISTQRLPFPSNAFDMAHCSRCLIPWTEFGGIYLLEIHRIVRPGGFWVL 301

Query: 304 SGPPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDK 332
           SGPPVNY  RWRGWNTT+E+Q+SDY KLQ LLTSMCF  Y +KDDIAVWQK +D +C+DK
Sbjct: 302 SGPPVNYNRRWRGWNTTMEDQKSDYNKLQSLLTSMCFKKYAQKDDIAVWQKLSDKSCYDK 361

BLAST of Cp4.1LG05g00460 vs. TAIR 10
Match: AT1G31850.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.3e-110
Identity = 222/605 (36.69%), Postives = 275/605 (45.45%), Query Frame = 0

Query: 4   KDGKLGHKQDK-NARVLPMTILLFVLCGFSFYLGGIFCSEKEGVNVNTAMDIENLVT--- 63
           K GK   + +K  +R+L +T+L    CGFSFYLGGIFCSE++ +    A D+    T   
Sbjct: 2   KSGKQSSQPEKGTSRILSLTVLFIAFCGFSFYLGGIFCSERDKI---VAKDVTRTTTKAV 61

Query: 64  -SSRDTVVSPLQLKPVVFQECSKEYQDYTPCTDPRKWKKYGFYRLTFMERHCPPMFERKE 123
            S ++   +P+Q+K V F EC  E+QDYTPCTDP++WKKYG +RL+F+ERHCPP++E+ E
Sbjct: 62  ASPKEPTATPIQIKSVSFPECGSEFQDYTPCTDPKRWKKYGVHRLSFLERHCPPVYEKNE 121

Query: 124 CLIPPPDGYKPPIRWPKSKDECWY------------------------------------ 183
           CLIPPPDGYKPPIRWPKS+++CWY                                    
Sbjct: 122 CLIPPPDGYKPPIRWPKSREQCWYRNVPYDWINKQKSNQHWLKKEGDKFHFPGGGTMFPR 181

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 182 GVSHYVDLMQDLIPEMKDGTVRTAIDTGCGVASWGGDLLDRGILSLSLAPRDNHEAQVQF 241

Query: 244 -------------------------------------------------RILRPGGLWVL 303
                                                            RI+RPGG WVL
Sbjct: 242 ALERGIPAILGIISTQRLPFPSNAFDMAHCSRCLIPWTEFGGIYLLEIHRIVRPGGFWVL 301

Query: 304 SGPPVNYENRWRGWNTTVEEQRSDYEKLQELLTSMCFTLYNKKDDIAVWQKSTDPNCFDK 332
           SGPPVNY  RWRGWNTT+E+Q+SDY KLQ LLTSMCF  Y +KDDIAVWQK +D +C+DK
Sbjct: 302 SGPPVNYNRRWRGWNTTMEDQKSDYNKLQSLLTSMCFKKYAQKDDIAVWQKLSDKSCYDK 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94II32.6e-11439.64Probable methyltransferase PMT21 OS=Arabidopsis thaliana OX=3702 GN=ERD3 PE=2 SV... [more]
Q9C6S73.3e-10936.69Probable methyltransferase PMT20 OS=Arabidopsis thaliana OX=3702 GN=At1g31850 PE... [more]
Q9SZX88.3e-2820.61Probable methyltransferase PMT17 OS=Arabidopsis thaliana OX=3702 GN=At4g10440 PE... [more]
Q9C8847.0e-2720.23Probable methyltransferase PMT18 OS=Arabidopsis thaliana OX=3702 GN=At1g33170 PE... [more]
O808445.0e-2519.74Probable methyltransferase PMT16 OS=Arabidopsis thaliana OX=3702 GN=At2g45750 PE... [more]
Match NameE-valueIdentityDescription
XP_023532722.11.76e-21255.15probable methyltransferase PMT21 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023... [more]
KAG7036230.15.82e-21154.82putative methyltransferase PMT21 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931156.12.36e-21054.65probable methyltransferase PMT21 [Cucurbita moschata] >XP_022931157.1 probable m... [more]
XP_022995627.15.49e-20954.32probable methyltransferase PMT21 isoform X1 [Cucurbita maxima] >XP_022995628.1 p... [more]
KAG6606289.12.98e-19453.26putative methyltransferase PMT21, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
A0A6J1ETJ51.14e-21054.65Methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111437422 PE=3 SV=1[more]
A0A6J1JZG32.66e-20954.32Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491107 PE=3 SV=1[more]
A0A0A0LE721.15e-19350.66Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G636940 PE=3 SV=1[more]
A0A1S3CCF83.27e-19350.50Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499239 PE=3 SV=1[more]
A0A5D3DBT43.27e-19350.50Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00... [more]
Match NameE-valueIdentityDescription
AT4G19120.11.8e-11539.64S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G19120.21.8e-11539.64S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G31850.12.3e-11036.69S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G31850.32.3e-11036.69S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G31850.22.3e-11036.69S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 85..143
e-value: 3.5E-16
score: 58.9
coord: 232..321
e-value: 1.5E-32
score: 112.9
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePANTHERPTHR10108SAM-DEPENDENT METHYLTRANSFERASEcoord: 142..233
coord: 1..143
coord: 233..327
NoneNo IPR availablePANTHERPTHR10108:SF1117S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-RELATEDcoord: 142..233
coord: 1..143
coord: 233..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g00460.1Cp4.1LG05g00460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0008168 methyltransferase activity