Cp4.1LG04g15990 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g15990
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Protein-L-isoaspartate O-methyltransferase
Location	Cp4.1LG04: 12393908 .. 12397735 (-)
RNA-Seq Expression	Cp4.1LG04g15990
Synteny	Cp4.1LG04g15990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTAATTAATTTATAGAATAATAGTGATTTTGTAGAGAGAGAGAGAGGGAGGCTAACTGGAAAAAACGAGAAGGGCGGAGAGAGAAATGAGTGTTACTCAATTATCTTTCCTCCCATTTGCTTATGGTTGCCGCTACTACATCACTCCCCTTCTCTCTACCGCCGATTTCTCACCGCCCTACCTCCGATCAATTCTTCTGCCCCTAAAACCACCGCCACCAGTATCGTCTCCGTCTTACCTCGACTTCGGTCTGCGCGTAATTGTCTTCTTTCCAGGATGAAGGTACTCTCTCCTTCTCTCTCTATCTACCGCTAATTCCTTCCCACCCATTCTCTCTTCTTCTTTACAAAATTTTCGTTTTCTGAAATTGGAGATTCAAGGTATTCTGTGTTTGTATGTGCACGGACGACCCAAATCTTTTTATTTTTGTATAGGATTTTCACCTAGGAGAAGGCTTTGATTATTATTAGCTGGTAATATATCGCCTTAATTGGAATTCGTAATTCATCTTTCTCTGTTCTCTGGATTTTTAATTGTTCTTTCAATCTGATGTATATGATCTTTTGCTTGTAAATTGTCGTTTAGTCCTCTCTTATGATACGAATTTGGTCCGTAGAGAACTAAATTTTCCCTTTGAAACTGCTTGATAAATTCATCGATGTTAGTTTTAATCATTCAAGGTATTTGTTTCCAATACATGTGGTTTTATTTCGTGTGGTTTTGGTAGTATATCTTATGCTGATATGTTTTCGTCCCTAACCCGCTCTGCTTCTGAGGCTGCGGAGCTTGATAAGGTACTTGCGTTGATGAGAATGAACTAAGGATCAGTTGGAAGATGGATGGATTTATAAATTAATGTTTAGAGATGGGAAGATAGCTTTAGGAACTCCTTGTGAATTGTGCTCTATTGGTGTGTTTCGGCGTGTTTCTGTTGAGGAATTCATTTTCAATTTTTGTTTCATGTCTTTGTTTTTGGTTGAAAGTTTGATCGTTCCATGTCATTTTAAATCACACTGCAGAGGTTCTGGAGTGGAAGTGGCATAGCTACAAACAAGGAAATGGTGAAGAATCTCCAGAACTATGGAGTGGTTGGGTCGAAGAAGGTGTCTGAAGTAATGGAGAGTATAGATAGGGCCTATTTTGTGCCCGATGATGCTCCACCTTATGTTGATACTCCCTTGCCAATAGGTTACAATGCCACTATATCTGCACCTCACATGCATGCAACCTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCGCGCTTTGGACGTTGGCTCAGGTTGTGCATCTAACTAAAAACTTCTATGCTCTGAAGATTGAATTGGTAACTTTTTTCATTGCTGTTGAATTCCTAATTATGTTTGACTTCGAGACATTCTGAATCTGGATTTCTAATCAGCNGAGGGTGGAGGGCGAGGTTTGGGATTGGATGGGCTTCAACTGTTGAAAGATGGATGGGATTTGATATCACTTTGGACATTTCGTTGAAGGTTAGCATTATCCCCCATATCGGTTGAAGAAGAGAACGAAGCATTCCTCGTAAGGGTGTGGAAACCTATTCCTAGTAGATGCATTTTAAAAACCGTGAGGCTGACGGCGATACGTAACGAGCCAAAGCGGACAGTATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAAAGCTAGACACCGGGTGGCGTGCCAGTGAGGGCGTTGGCCCCCAAGGGGATGGATTGTGAGATCCCGCATCGGTTGGAGAAGAGAATGAAACATTTCTTATGAGAGTGAAAAGAAAAAGAAAAAAAATTGAAATGCAGAAGAATGATTCTGGTCTTTCCTGTAAGGGGTTTAGTGTGCTCTAAACATGCACTACCCAAGTGAAAGTGCAAGTTGGTTACTCTAAAAAGATACAACTCTATTACCTTTTCACATTTGAAATTTGCATTTTCAACTTCAAATGCCATTTCCTTGACCTTCCTCGGTCTTCCTATATACTTAAGTTGAATTTACATGTTTGATTTCTCCTGGGCAGGAACGGGATATTTGACTGCATGCTTTGCGTTAATGATTGGACCTGAAGGTCGTGCAGTTGGTGTGGAACATATTCCTGAGCTGGTGGCTTCATCAATCGAGAATATTAATAAAAGTGCTGCCGCTCCTTTACTGAAAGAAGGTTCCCTCTCTCTACACGTTGCTGGTATGTTTGTCTTCTTTCCCTTTTCTAGAGCCATAGATTTTTCTTTTGATTTAGAATAAAGAACTCTGTTCCTTAGTTTAATTGGCCGTAAAGAGATGAAGGATGTAGTGAAGTGACCGTTAAGCAATTTTCGTGCACCCGTTATGCACTGGAATGGTATATTCTTGAAAGTACAAGGTAAGTTCGAGATATTTTCTAAAAGTAGTGCTTTACCCTTTGAGCGAAACACTTAGAAAACAAAACACTGTAACTAGAGTTCTATTACTACAGAGTGCTTCGAATTTTTGTTTAAAATATCTCAAGCTACTTCTTGGGTAGATAACTTAAAGCCATTCTAAAAAATCCTTAAATTTAATTAAGAACTTCTTACTACTACGTAAGTCATGCTAAACCCGTCTAAAACGAGATTTCTCCCCTTTGAAACTAGATGAGGAGAAAATTTGAGGAGCTCAATTCTCCTTTAGAAATCCATTAGTGTCGCCTCCCTCCCCAGCGCTCACACTTTAATCTTAACAATGTTCTTCGGTGAACTGTTGTGGCTAATGAGGGTGAGAAGATCTAACATGGCAAAAGTAGAATGTGGGTATTAATTCTAGGGCGCGTTTGTCCTACTCGATGATGTGTGTTGTTAATGAAAGTTTGTGGTTACTTTGTTATTGGATGGTTGTAGATGGCAGGCAGGGCTGGCCAGAGTGTGCACCTTATGATGCTATTCATGTTGGGGCAGCAGCTGCTGAAATTCCGCCGGCTCTCATTGACCAGTTAAAGCCAGGTGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGATGGTTGTGGATAAAGATGCCGATGGCTCTGTTAACATCCACAACGAGACTTCTGTTCGTTATGTGCCTCTTACCAGCCGAGAAGCTCAGCTACGTGACAACTGATCGAGTTTACTCATCCCATGCAGATGCTGCTTTTATCAAGAACGTTGTATAGAAACTTTAAGAGATCTTTTGCGCATCTAATTGAATCATATCATCCTAGATGTGTGTACTTAGTCCAAATAAAGATGTTATATCCTTTGTTTTCGACAATCACAGACGATAAACAGAAGATGACTCGTTAGAACTTACAAAATTTCATTGACAGGGTTGATAATTCGATTGTATTCTTTTTGTTGTATCATTTGTTTTTGGTCACGAAAGACCCTCTCATAACCCCTCTCTCTCTAGTGAACGCCATATCTCTCTCAGAAATGCAATAACTTTAATTGAAAAAGACAATCCCATCCAGAGAGAGTAATAATTCAAGAAGAAGATGGACTACGAAACTTTTAACTCATACAAGATTCAAATTAAGAACAGACTTGAAATTTTAAAGGCATAAAGGACATCAAATTATACCTTTACGAAGGTGGCTAAACAATGGCACTACAAAAACTCACCTATACTTCCTGGAAACAAAAAAGGTAACACATAACAAGAGAAAAAGGTCCATGGATTCATCTTGGTTCACCTCAGAAGTTGCGCCTAGGTCTTTCCTCTGCAACATTTACTCTGATTGCCCTTCCATCTATACTCTGTACAATTCAAATTTCACAACCTTTTGTTAGCATTTGATGATGAGATTCAAGAACTGGAAGAGTATGTTTGGAAT

mRNA sequence

TTTAATTAATTTATAGAATAATAGTGATTTTGTAGAGAGAGAGAGAGGGAGGCTAACTGGAAAAAACGAGAAGGGCGGAGAGAGAAATGAGTGTTACTCAATTATCTTTCCTCCCATTTGCTTATGGTTGCCGCTACTACATCACTCCCCTTCTCTCTACCGCCGATTTCTCACCGCCCTACCTCCGATCAATTCTTCTGCCCCTAAAACCACCGCCACCAGTATCGTCTCCGTCTTACCTCGACTTCGGTCTGCGCGTAATTGTCTTCTTTCCAGGATGAAGAGGTTCTGGAGTGGAAGTGGCATAGCTACAAACAAGGAAATGGTGAAGAATCTCCAGAACTATGGAGTGGTTGGGTCGAAGAAGGTGTCTGAAGTAATGGAGAGTATAGATAGGGCCTATTTTGTGCCCGATGATGCTCCACCTTATGTTGATACTCCCTTGCCAATAGGTTACAATGCCACTATATCTGCACCTCACATGCATGCAACCTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCGCGCTTTGGACGTTGGCTCAGGAACGGGATATTTGACTGCATGCTTTGCGTTAATGATTGGACCTGAAGGTCGTGCAGTTGGTGTGGAACATATTCCTGAGCTGGTGGCTTCATCAATCGAGAATATTAATAAAAGTGCTGCCGCTCCTTTACTGAAAGAAGGTTCCCTCTCTCTACACGTTGCTGATGGCAGGCAGGGCTGGCCAGAGTGTGCACCTTATGATGCTATTCATGTTGGGGCAGCAGCTGCTGAAATTCCGCCGGCTCTCATTGACCAGTTAAAGCCAGGTGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGATGGTTGTGGATAAAGATGCCGATGGCTCTGTTAACATCCACAACGAGACTTCTGTTCGTTATGTGCCTCTTACCAGCCGAGAAGCTCAGCTACGTGACAACTGATCGAGTTTACTCATCCCATGCAGATGCTGCTTTTATCAAGAACGTTGTATAGAAACTTTAAGAGATCTTTTGCGCATCTAATTGAATCATATCATCCTAGATGTGTGTACTTAGTCCAAATAAAGATGTTATATCCTTTGTTTTCGACAATCACAGACGATAAACAGAAGATGACTCGTTAGAACTTACAAAATTTCATTGACAGGGTTGATAATTCGATTGTATTCTTTTTGTTGTATCATTTGTTTTTGGTCACGAAAGACCCTCTCATAACCCCTCTCTCTCTAGTGAACGCCATATCTCTCTCAGAAATGCAATAACTTTAATTGAAAAAGACAATCCCATCCAGAGAGAGTAATAATTCAAGAAGAAGATGGACTACGAAACTTTTAACTCATACAAGATTCAAATTAAGAACAGACTTGAAATTTTAAAGGCATAAAGGACATCAAATTATACCTTTACGAAGGTGGCTAAACAATGGCACTACAAAAACTCACCTATACTTCCTGGAAACAAAAAAGGTAACACATAACAAGAGAAAAAGGTCCATGGATTCATCTTGGTTCACCTCAGAAGTTGCGCCTAGGTCTTTCCTCTGCAACATTTACTCTGATTGCCCTTCCATCTATACTCTGTACAATTCAAATTTCACAACCTTTTGTTAGCATTTGATGATGAGATTCAAGAACTGGAAGAGTATGTTTGGAAT

Coding sequence (CDS)

ATGAAGAGGTTCTGGAGTGGAAGTGGCATAGCTACAAACAAGGAAATGGTGAAGAATCTCCAGAACTATGGAGTGGTTGGGTCGAAGAAGGTGTCTGAAGTAATGGAGAGTATAGATAGGGCCTATTTTGTGCCCGATGATGCTCCACCTTATGTTGATACTCCCTTGCCAATAGGTTACAATGCCACTATATCTGCACCTCACATGCATGCAACCTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCGCGCTTTGGACGTTGGCTCAGGAACGGGATATTTGACTGCATGCTTTGCGTTAATGATTGGACCTGAAGGTCGTGCAGTTGGTGTGGAACATATTCCTGAGCTGGTGGCTTCATCAATCGAGAATATTAATAAAAGTGCTGCCGCTCCTTTACTGAAAGAAGGTTCCCTCTCTCTACACGTTGCTGATGGCAGGCAGGGCTGGCCAGAGTGTGCACCTTATGATGCTATTCATGTTGGGGCAGCAGCTGCTGAAATTCCGCCGGCTCTCATTGACCAGTTAAAGCCAGGTGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGATGGTTGTGGATAAAGATGCCGATGGCTCTGTTAACATCCACAACGAGACTTCTGTTCGTTATGTGCCTCTTACCAGCCGAGAAGCTCAGCTACGTGACAACTGA

Protein sequence

MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN

Homology

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BLAST of Cp4.1LG04g15990 vs. ExPASy Swiss-Prot
Match: Q42539 (Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PIMT1 PE=1 SV=3)

HSP 1 Score: 357.1 bits (915), Expect = 1.5e-97
Identity = 173/228 (75.88%), Postives = 199/228 (87.28%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MK+FWS S I  NK MV+NLQN+G+V S +V++ ME++DR  FV D +  YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           N TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+M+G EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASS++NI  SAA+P LKEGSL++HV DGRQGW E APYDAIHVGAAA EIP ALIDQLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLR 229
           PGGR+VIPVGNIFQDL VVDK++DGSV+I +ETSVRYVPLTSREAQLR
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLR 228

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BLAST of Cp4.1LG04g15990 vs. ExPASy Swiss-Prot
Match: Q43209 (Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum OX=4565 GN=PCM PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-91
Identity = 158/230 (68.70%), Postives = 192/230 (83.48%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           M +FW+   +  N  +V+ L+ YGVV + KV+EVME+IDRA FVP+   PY D+P+PIGY
Sbjct: 1   MAQFWAEGSLEKNNALVEYLKQYGVVRTDKVAEVMETIDRALFVPEGFTPYTDSPMPIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCL+LL+ +LQPGM ALDVGSG+GYLTACFA+M+GPEGRAVG+EHIPEL
Sbjct: 61  NATISAPHMHATCLELLKDYLQPGMHALDVGSGSGYLTACFAMMVGPEGRAVGIEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           V +S EN+ +SAAA L+K+GSLS HV+DGR GWP+ APYDAIHVGAAA EIP  L++QLK
Sbjct: 121 VVASTENVERSAAAALMKDGSLSFHVSDGRLGWPDAAPYDAIHVGAAAPEIPRPLLEQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 231
           PGGRMVIPVG   QDL V+DK ADGS ++ N+ SVRYVPLTSR AQL+D+
Sbjct: 181 PGGRMVIPVGTYSQDLQVIDKSADGSTSVRNDASVRYVPLTSRSAQLQDS 230

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BLAST of Cp4.1LG04g15990 vs. ExPASy Swiss-Prot
Match: Q64J17 (Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PIMT2 PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-90
Identity = 161/227 (70.93%), Postives = 189/227 (83.26%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           M++F SG+G +  + MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGY
Sbjct: 80  MEQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGY 139

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPEL
Sbjct: 140 NATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPEL 199

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           V  SI+NI KS AA  LK+GSLSLHV DGR+GW E APYDAIHVGAAA+EIP  L+DQLK
Sbjct: 200 VDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLK 259

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQL 228
           PGGRMVIP+G  FQ+L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 260 PGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 306

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BLAST of Cp4.1LG04g15990 vs. ExPASy Swiss-Prot
Match: Q5F3N1 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus OX=9031 GN=PCMT1 PE=2 SV=3)

HSP 1 Score: 216.5 bits (550), Expect = 3.2e-55
Identity = 116/221 (52.49%), Postives = 150/221 (67.87%), Query Frame = 0


Query: 8   SGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYNATISAP 67
           SG A++ E++ NL+  G++ S KV EVM + DR ++   +  PY+D+P  IG+ ATISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKSDKVFEVMLATDRCHYAKYN--PYMDSPQSIGFQATISAP 64

Query: 68  HMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIEN 127
           HMHA  L+LL   L  G +ALDVGSG+G LTACF+ M+GP+G+ VG++HI ELV  SI N
Sbjct: 65  HMHAYALELLSDQLHEGAKALDVGSGSGILTACFSRMVGPKGQVVGIDHIKELVDDSINN 124

Query: 128 INKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVI 187
           + K     LL  G + L V DGR G+ E APYDAIHVGAAA  +P ALIDQLKPGGR+++
Sbjct: 125 VKKDDPT-LLSSGRVKLIVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLIL 184

Query: 188 PVGNIFQDLMV--VDKDADGSVNIHNETSVRYVPLTSREAQ 227
           PVG    + M+   DK  DGSV +     V YVPLT +E Q
Sbjct: 185 PVGPAGGNQMLEQYDKLEDGSVKMKPLMGVIYVPLTDKEKQ 222

[Close]

BLAST of Cp4.1LG04g15990 vs. ExPASy Swiss-Prot
Match: P80895 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa OX=9823 GN=PCMT1 PE=1 SV=3)

HSP 1 Score: 214.9 bits (546), Expect = 9.4e-55
Identity = 115/221 (52.04%), Postives = 149/221 (67.42%), Query Frame = 0


Query: 8   SGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYNATISAP 67
           SG A++ E++ NL+  G++ + KV EVM + DR+++      PY+D+P  IG+ ATISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYA--KCNPYMDSPQSIGFQATISAP 64

Query: 68  HMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIEN 127
           HMHA  L+LL   L  G +ALDVGSG+G LTACFA M+GP G+ +G++HI ELV  SI N
Sbjct: 65  HMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGPSGKVIGIDHIKELVDDSINN 124

Query: 128 INKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVI 187
           + K     LL  G + L V DGR G+ E APYDAIHVGAAA  +P ALIDQLKPGGR+++
Sbjct: 125 VRKDDPM-LLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLIL 184

Query: 188 PVGNIFQDLMV--VDKDADGSVNIHNETSVRYVPLTSREAQ 227
           PVG    + M+   DK  DGSV +     V YVPLT +E Q
Sbjct: 185 PVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

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BLAST of Cp4.1LG04g15990 vs. NCBI nr
Match: XP_023530766.1 (protein-L-isoaspartate O-methyltransferase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 466 bits (1198), Expect = 1.55e-165
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY
Sbjct: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL
Sbjct: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230

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BLAST of Cp4.1LG04g15990 vs. NCBI nr
Match: XP_023002159.1 (protein-L-isoaspartate O-methyltransferase 1 [Cucurbita maxima])

HSP 1 Score: 460 bits (1184), Expect = 2.12e-163
Identity = 226/230 (98.26%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRA+FVPDDAPPYVDTPLPIGY
Sbjct: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GPEGRAVGVEHIPEL
Sbjct: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDLMVVDKD DGSVNIHNETSVRYVPLTSREAQLRD+
Sbjct: 181 PGGRMVIPVGNIFQDLMVVDKDVDGSVNIHNETSVRYVPLTSREAQLRDS 230

[Close]

BLAST of Cp4.1LG04g15990 vs. NCBI nr
Match: XP_022931740.1 (protein-L-isoaspartate O-methyltransferase 1 [Cucurbita moschata])

HSP 1 Score: 460 bits (1183), Expect = 3.01e-163
Identity = 227/230 (98.70%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRA+FVPDDAPPYVDTPLPIGY
Sbjct: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL
Sbjct: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPEC+ YDAIHVGAAAAEIPPALIDQLK
Sbjct: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSSYDAIHVGAAAAEIPPALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230

[Close]

BLAST of Cp4.1LG04g15990 vs. NCBI nr
Match: KAG6587436.1 (Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 461 bits (1186), Expect = 3.03e-162
Identity = 227/229 (99.13%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 2   KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYN 61
           KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRA+FVPDDAPPYVDTPLPIGYN
Sbjct: 95  KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 154

Query: 62  ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 121
           ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV
Sbjct: 155 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 214

Query: 122 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKP 181
           ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPEC+PYDAIHVGAAAAEIPPALIDQLKP
Sbjct: 215 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 274

Query: 182 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 275 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 323

[Close]

BLAST of Cp4.1LG04g15990 vs. NCBI nr
Match: KAG7021419.1 (Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461 bits (1186), Expect = 8.21e-162
Identity = 227/229 (99.13%), Postives = 229/229 (100.00%), Query Frame = 0


Query: 2   KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYN 61
           KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRA+FVPDDAPPYVDTPLPIGYN
Sbjct: 123 KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 182

Query: 62  ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 121
           ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV
Sbjct: 183 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 242

Query: 122 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKP 181
           ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPEC+PYDAIHVGAAAAEIPPALIDQLKP
Sbjct: 243 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 302

Query: 182 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 303 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 351

[Close]

BLAST of Cp4.1LG04g15990 vs. ExPASy TrEMBL
Match: A0A6J1KPN1 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496101 PE=3 SV=1)

HSP 1 Score: 460 bits (1184), Expect = 1.02e-163
Identity = 226/230 (98.26%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRA+FVPDDAPPYVDTPLPIGY
Sbjct: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GPEGRAVGVEHIPEL
Sbjct: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDLMVVDKD DGSVNIHNETSVRYVPLTSREAQLRD+
Sbjct: 181 PGGRMVIPVGNIFQDLMVVDKDVDGSVNIHNETSVRYVPLTSREAQLRDS 230

[Close]

BLAST of Cp4.1LG04g15990 vs. ExPASy TrEMBL
Match: A0A6J1F0A7 (protein-L-isoaspartate O-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111437899 PE=3 SV=1)

HSP 1 Score: 460 bits (1183), Expect = 1.46e-163
Identity = 227/230 (98.70%), Postives = 229/230 (99.57%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRA+FVPDDAPPYVDTPLPIGY
Sbjct: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL
Sbjct: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPEC+ YDAIHVGAAAAEIPPALIDQLK
Sbjct: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSSYDAIHVGAAAAEIPPALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230

[Close]

BLAST of Cp4.1LG04g15990 vs. ExPASy TrEMBL
Match: A0A6J1JIJ8 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484770 PE=3 SV=1)

HSP 1 Score: 430 bits (1105), Expect = 1.69e-151
Identity = 209/230 (90.87%), Postives = 220/230 (95.65%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           +KRFWSGSGI TNKEMV+NLQ+YGVV SKKVSEVMES+DRA+FVPD  PPYVDTPLPIGY
Sbjct: 12  LKRFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGY 71

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GPEGRAVGVEHIPEL
Sbjct: 72  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 131

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSI+NI KSAAAPLLK+GSLS+HV DGRQGW ECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 132 VASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 191

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDL VVDKD DGS+NIH+ETSVRYVPLTSREAQL DN
Sbjct: 192 PGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGDN 241

[Close]

BLAST of Cp4.1LG04g15990 vs. ExPASy TrEMBL
Match: A0A6J1E441 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111429755 PE=3 SV=1)

HSP 1 Score: 427 bits (1099), Expect = 1.39e-150
Identity = 208/230 (90.43%), Postives = 219/230 (95.22%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           +KRFWSGSGI TNKEMV+NLQNYGVV SKKVSEVMES+DRA+FVPD  PPYVDTP+PIGY
Sbjct: 12  LKRFWSGSGIGTNKEMVENLQNYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPVPIGY 71

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GP+GRAVGVEHIPEL
Sbjct: 72  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPKGRAVGVEHIPEL 131

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSI+NI KSAAAPLLKEGSLS+HV DGRQGW ECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 132 VASSIKNIEKSAAAPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 191

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDL VVDKD DGS+NI +ETSVRYVPLTSREAQL DN
Sbjct: 192 PGGRMVIPVGNIFQDLKVVDKDTDGSINIRDETSVRYVPLTSREAQLGDN 241

[Close]

BLAST of Cp4.1LG04g15990 vs. ExPASy TrEMBL
Match: A0A6J1JD88 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484770 PE=3 SV=1)

HSP 1 Score: 429 bits (1104), Expect = 2.46e-150
Identity = 209/230 (90.87%), Postives = 220/230 (95.65%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           M+RFWSGSGI TNKEMV+NLQ+YGVV SKKVSEVMES+DRA+FVPD  PPYVDTPLPIGY
Sbjct: 77  MERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGY 136

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GPEGRAVGVEHIPEL
Sbjct: 137 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 196

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASSI+NI KSAAAPLLK+GSLS+HV DGRQGW ECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 197 VASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 256

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 230
           PGGRMVIPVGNIFQDL VVDKD DGS+NIH+ETSVRYVPLTSREAQL DN
Sbjct: 257 PGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGDN 306

[Close]

BLAST of Cp4.1LG04g15990 vs. TAIR 10
Match: AT3G48330.1 (protein-l-isoaspartate methyltransferase 1 )

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-98
Identity = 173/228 (75.88%), Postives = 199/228 (87.28%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MK+FWS S I  NK MV+NLQN+G+V S +V++ ME++DR  FV D +  YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           N TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+M+G EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASS++NI  SAA+P LKEGSL++HV DGRQGW E APYDAIHVGAAA EIP ALIDQLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLR 229
           PGGR+VIPVGNIFQDL VVDK++DGSV+I +ETSVRYVPLTSREAQLR
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLR 228

[Close]

BLAST of Cp4.1LG04g15990 vs. TAIR 10
Match: AT3G48330.2 (protein-l-isoaspartate methyltransferase 1 )

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-98
Identity = 173/228 (75.88%), Postives = 199/228 (87.28%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           MK+FWS S I  NK MV+NLQN+G+V S +V++ ME++DR  FV D +  YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           N TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+M+G EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           VASS++NI  SAA+P LKEGSL++HV DGRQGW E APYDAIHVGAAA EIP ALIDQLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLR 229
           PGGR+VIPVGNIFQDL VVDK++DGSV+I +ETSVRYVPLTSREAQLR
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLR 228

[Close]

BLAST of Cp4.1LG04g15990 vs. TAIR 10
Match: AT5G50240.1 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 333.6 bits (854), Expect = 1.3e-91
Identity = 161/227 (70.93%), Postives = 189/227 (83.26%), Query Frame = 0


Query: 1   MKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGY 60
           M++F SG+G +  + MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGY
Sbjct: 80  MEQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGY 139

Query: 61  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 120
           NATISAPHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPEL
Sbjct: 140 NATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPEL 199

Query: 121 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLK 180
           V  SI+NI KS AA  LK+GSLSLHV DGR+GW E APYDAIHVGAAA+EIP  L+DQLK
Sbjct: 200 VDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLK 259

Query: 181 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQL 228
           PGGRMVIP+G  FQ+L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 260 PGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 306

[Close]

BLAST of Cp4.1LG04g15990 vs. TAIR 10
Match: AT5G50240.2 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 329.3 bits (843), Expect = 2.4e-90
Identity = 159/222 (71.62%), Postives = 185/222 (83.33%), Query Frame = 0


Query: 6   SGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYNATIS 65
           SG+G +  + MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGYNATIS
Sbjct: 82  SGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYNATIS 141

Query: 66  APHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSI 125
           APHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPELV  SI
Sbjct: 142 APHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELVDMSI 201

Query: 126 ENINKSAAAPLLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKPGGRM 185
           +NI KS AA  LK+GSLSLHV DGR+GW E APYDAIHVGAAA+EIP  L+DQLKPGGRM
Sbjct: 202 KNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRM 261

Query: 186 VIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQL 228
           VIP+G  FQ+L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 262 VIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 303

[Close]

BLAST of Cp4.1LG04g15990 vs. TAIR 10
Match: AT5G50240.3 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 324.3 bits (830), Expect = 7.8e-89
Identity = 156/212 (73.58%), Postives = 179/212 (84.43%), Query Frame = 0


Query: 16  MVKNLQNYGVVGSKKVSEVMESIDRAYFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQ 75
           MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGYNATISAPHMHATCLQ
Sbjct: 1   MVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYNATISAPHMHATCLQ 60

Query: 76  LLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAP 135
           LLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPELV  SI+NI KS AA 
Sbjct: 61  LLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELVDMSIKNIEKSVAAS 120

Query: 136 LLKEGSLSLHVADGRQGWPECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQD 195
            LK+GSLSLHV DGR+GW E APYDAIHVGAAA+EIP  L+DQLKPGGRMVIP+G  FQ+
Sbjct: 121 FLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRMVIPLGTYFQE 180

Query: 196 LMVVDKDADGSVNIHNETSVRYVPLTSREAQL 228
           L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 181 LKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 212

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g15990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42539	1.5e-97	75.88	Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q43209	1.6e-91	68.70	Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum OX=4565 GN=PCM P...	[more]
Q64J17	1.8e-90	70.93	Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q5F3N1	3.2e-55	52.49	Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus OX=9031...	[more]
P80895	9.4e-55	52.04	Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa OX=9823 GN...	[more]

BLAST of Cp4.1LG04g15990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530766.1	1.55e-165	100.00	protein-L-isoaspartate O-methyltransferase 1-like isoform X1 [Cucurbita pepo sub...	[more]
XP_023002159.1	2.12e-163	98.26	protein-L-isoaspartate O-methyltransferase 1 [Cucurbita maxima]	[more]
XP_022931740.1	3.01e-163	98.70	protein-L-isoaspartate O-methyltransferase 1 [Cucurbita moschata]	[more]
KAG6587436.1	3.03e-162	99.13	Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma su...	[more]
KAG7021419.1	8.21e-162	99.13	Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma su...	[more]

BLAST of Cp4.1LG04g15990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KPN1	1.02e-163	98.26	Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1F0A7	1.46e-163	98.70	protein-L-isoaspartate O-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1JIJ8	1.69e-151	90.87	Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1E441	1.39e-150	90.43	Protein-L-isoaspartate O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JD88	2.46e-150	90.87	Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Cp4.1LG04g15990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48330.1	1.1e-98	75.88	protein-l-isoaspartate methyltransferase 1	[more]
AT3G48330.2	1.1e-98	75.88	protein-l-isoaspartate methyltransferase 1	[more]
AT5G50240.1	1.3e-91	70.93	protein-l-isoaspartate methyltransferase 2	[more]
AT5G50240.2	2.4e-90	71.62	protein-l-isoaspartate methyltransferase 2	[more]
AT5G50240.3	7.8e-89	73.58	protein-l-isoaspartate methyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 5..230 e-value: 2.6E-80 score: 271.4
None	No IPR available	PFAM	PF01135	PCMT	coord: 12..224 e-value: 7.9E-85 score: 283.9
None	No IPR available	PANTHER	PTHR11579:SF26	L-ISOASPARTATE METHYLTRANSFERASE	coord: 3..230
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 86..189 e-value: 9.48698E-7 score: 44.3431
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	TIGRFAM	TIGR00080	TIGR00080	coord: 12..226 e-value: 9.9E-66 score: 219.6
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	PANTHER	PTHR11579	PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE	coord: 3..230
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	PROSITE	PS01279	PCMT	coord: 147..162
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 12..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g15990.1	Cp4.1LG04g15990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006479	protein methylation
biological_process	GO:0006464	cellular protein modification process
molecular_function	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity