Cp4.1LG04g15950 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g15950
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCytochrome P450
LocationCp4.1LG04: 12371286 .. 12375150 (+)
RNA-Seq ExpressionCp4.1LG04g15950
SyntenyCp4.1LG04g15950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAACTCTTCTTCTTCTTCAATCATCACTTCATCCAATTTTGTTCGGTGGTTGCGTCCTTGTCCTGATCTTCCTTCTCCAAAAATGGCTGCTTTCCAATCAAAACTCATCAAAAGCCTCGCCGCCATCGCCGCCAAAGCATCCAATCTTCGGCCACCTTTTGAAGCTTGGCCGCCTTCCTCATATTACTCTCCAAAACTATGCTCGCCGCTATGGTCCTCTGTTTCTCCTTCACTTGGGCGCCAAGCCCACCATCGTGGTCTCTTCTCCCCAACTGGCTCGTGATATCATGAAGACCCACGACCTTAGCTTTGCCAATCGACCCAAATCAAGCATGGCTGATAAGCTTCTTTATGGGTCAAAAGATATAGCGATGTCTCCTTATGGTGAATATTGGAGGCGGATGAGAAGCATCGGCGTGCTCCGTCTTTTGAGCCACAACAGTGTCCAATCGTTTTGTTGTGTTAGAGAACAAGAAGTGAAGGCCATGATCGACAACATCCAACGCAATCCACTGTCGGTTAATTTCACTGAACTCTTCTCTGGACTGACCAACGACGTGATTTGCAGGGTGGCCTTTGGGAGAAAATATGGGATTGGTCAAGATGGGTCGAAGCTTAGGTCCCTTCTCTTGCAATTTGGGGAGTTAATGGCGAGTTTTATCATTCGAGACTACGTTCCATTGCTGGGTTGGATCGATGGCATTACTGGGTTCGACGCCAAAGCCAAGAGGGTAGCCAAAGAGCTGGACATTTTGTTAGATGAAGTGATTCAAGATCATATTCATTTACAGAACAGAGCAGAGCACAAGGACTTGGTGGATATTCTACTCTCTATCCAAGTAGAGAACTCCACTGGTTCCCCCCTTCAAATGGAGAGCATAAAAGCTTTAATCTTGGTAAGGCCCGGTTACTAATTACGAATCTCCACACCGGTATGATATTCTCCGCTTTGAGCATAAGCTCATGGCCTTGCTTTGGGCTTCCCCAAAACGGCTCATAGGAGATGTATTCTTTACTTATAGACCTATGATCGTTCCATAAATTATCCCCTCGAACAAAATACACTATAAAGCCTCTCTTGAAGCCCTCGAATAGCCTCCTCTTAATTGAGGCTCGACTCCTTCTTTAGAGTCCTCGAACGAAGTACACCCTTTGTTCGACACTACTATACCTTCGAGGCTCACAACTTCTTTGTTCGACACATGAGTATTATATTGACGTGAGGAAGTTAAGAGCATGACTCTCATAACATTTTAGGAATAACTATTCTCCACAATGGTACGATGTTGTCCACTTTGAGTATAAACTCTAGTGGGTTTGCTTTGCGTTTCCCCAAAATGACTCGTACCAATGGATCATTACTAAATTAGGCAATGTGGAACTCACTCCTAATAACAGGCCCAGCATTTGTTCTTCTCGAGTTATCAACGTTGTGATTTTGATTACAGGACATGTTTGCAGCAGGCGTAGACTCGACGTACGCAGCATTAGAATGGGCAATGTCAGAATTGTTGAAACACCCAGAAAGCATGAAGAAATTGAAGGGTGAGATGAGGGAAATAATGGGTGGGAAAGGCTATGCGGGGGAGGAGGATATAGAAAAGATGCCTTACTTGAAGGCAATAATCAAGGAAACGTTGCGGCTACACCCACCACTCCCGCTTCTGTTCAGAGAATCGAGTGAATACATTAAGGTGAAGGGATACGATATTAATCCCGGCACTCGGGTAATCATCAATGCATGGGCCATCGGAAGAGACCCAGAGGTGTGGGAAGAAGCAGAGGAGTTTCGACCGGAGAGGTTCATGAAGAGTTCGATGGATTTTAAGGGTCAGAGTTTGGAGTTGATCCCGTTCGGTGCAGGGCGAAGAGGGTGTCCTGGAATTGCGTTTGCAATGGCAATGATTGAGATGGCTTTGGCTAACGTGGTGTATAAATTTGAGTGGAAGTTGCCTAATGGAGCTGCCGGGGAGGATTTGGACATGTCTGGAGTGTTTGGGTTAGCCACTCATAGGGAGTATCCACTTGTGGCAATCGCAACTTCTTGTCAGAGGGACTCGACGTCGTACAACTCATAGTACGTGGACATCAACTGTTTATCTACTTCTCATCGACTATCTTCTTGTTTGATCTTCTTTCTATAAAATATTATTAAATAAAAGCGGATTGACGCTCTAGAACGACATTCCTCTGTGACAGTGCAGTTCCCGAGTACCTTCGCACCTCGACCGATACCTGAAAAAGATAGTGAAAGAATGGTGAGTACAAAAATATACTCAGTAATCAACCTACTTGTAGGCTCCTCTTCGAGTAGATTTCCACTGACTTTTCTTTGGTCTATAAGAAGATTGAGCTCACTCTTACATTGCTCTGAGCTATTGTGTAGGTCTTGAAATTCCAATTGATTGTCTATCCGTGTAGTCCCTAATCCTTCTTTGAGACTGGTTTACGGTGTCACGGCGTCGACCTTGACTTGTTCCTACACTTAATAGACGTGTTCATACTAAATCACCTACCTGCGTAGTCCCTAATCTCTTCCAACACCGATTTACGAAGTTGCAACGTCGGCGTTGACTCGTTTCTACACTCGATAGGGGCTCTCATGCTGACACCTTCTCGTGTAGCCCCTAGTCCAACCAAGACTAGTTTACAGGTTCGCATGTTTCTACACTCAATAGGAGTCCATGTCTTGAGCTAACTACCCTTGTAGTCACTAATTTCTTTCGAGAACTGGTTTAACATGATTAGAGCGTCAACCCTGTCCTGACTCTACACTCAATAGTTACTTAACTAGTTTCGTGATTCTGTAGGGTACTTAAATCATGTTAATACATTGTGGAGGTCTACTAGGTTCTTAGGGTGTTCATGCGGTTTAAATTCCATTTTCTCATTTACAGGACATTCTAGGTTAGCCCTCGGCTTAACCAATCCTTTGATGCACATAGGTCTAACCTCTAGTTCTGCATGTAAACTGACTAGATATAGCGCCCTAATCTTAACTGTGATCTTGATGCATTCTCTATTAGGAAAATATTTCTATCAATCCTCTCTAACATATAAATCATGTCTACCTGTTCACATATATTATTTAAAAAGGTATCTACCGTTGCATGAAAATTATAACATAAATCACACAATCAAACAAGCTCAACGGGGAGATAAAATCTATCAATCACCTAGAGCACATATCAGAACATCCCTACCTCCATCATACCACAAATGAATGGCGTTAGTTAAATTAGGCATCCTTGTCAAATCTCTTGTTCTAAGGACAATTTGACCCACGTAATACAAGCCATTAGCTAGATGGGATAAGGGTGAAGGGGTCGCGTCTTCAACATGGGTGACTTTATGAACCTAAGAGGTGCCAACTCACCCCTTAAGTAAGACACGTAAGACACCATATAGTCTCATAAGAGCGTTTATTATGGGGTGACCATTCTAAAGCCTAGATTCTCAATTTCCTCGGATTTGATTCCTTAAATCATTATATATATATATATATATAGACACACACACACACACAGCCACATCTTTCCATTTCTAAATCAGGCGAGGGAGAGAGAGAGCAATGGAGACTCTTCAAACTCTTCAGACTCTTCTTCTTCATCCAATTTCGTTCACTGCTTTCGTCCTTATCCTGATCTTCCTTCTCCAAAAATGGCTGCTTTCCAATCAAAACACATCAAAAGCCTCACCACCATCTCCGCCAAAGCATCCAATCTTCGGCCACCTTTTGAAGCTCGGCCGCCTTCCTCATATCACTCTCCAAAACTATGCTCGCCGCTATGGTCCTCTGTTTCTCCTTCACTTGGGCGCCAAGCCCACCATCGTGGTCTCTTCTCCCCAACTGGCTCGTGA

mRNA sequence

ATGGAAACTCTTCTTCTTCTTCAATCATCACTTCATCCAATTTTGTTCGGTGGTTGCGTCCTTGTCCTGATCTTCCTTCTCCAAAAATGGCTGCTTTCCAATCAAAACTCATCAAAAGCCTCGCCGCCATCGCCGCCAAAGCATCCAATCTTCGGCCACCTTTTGAAGCTTGGCCGCCTTCCTCATATTACTCTCCAAAACTATGCTCGCCGCTATGGTCCTCTGTTTCTCCTTCACTTGGGCGCCAAGCCCACCATCGTGGTCTCTTCTCCCCAACTGGCTCGTGATATCATGAAGACCCACGACCTTAGCTTTGCCAATCGACCCAAATCAAGCATGGCTGATAAGCTTCTTTATGGGTCAAAAGATATAGCGATGTCTCCTTATGGTGAATATTGGAGGCGGATGAGAAGCATCGGCGTGCTCCGTCTTTTGAGCCACAACAGTGTCCAATCGTTTTGTTGTGTTAGAGAACAAGAAGTGAAGGCCATGATCGACAACATCCAACGCAATCCACTGTCGGTTAATTTCACTGAACTCTTCTCTGGACTGACCAACGACGTGATTTGCAGGGTGGCCTTTGGGAGAAAATATGGGATTGGTCAAGATGGGTCGAAGCTTAGGTCCCTTCTCTTGCAATTTGGGGAGTTAATGGCGAGTTTTATCATTCGAGACTACGTTCCATTGCTGGGTTGGATCGATGGCATTACTGGGTTCGACGCCAAAGCCAAGAGGGTAGCCAAAGAGCTGGACATTTTGTTAGATGAAGTGATTCAAGATCATATTCATTTACAGAACAGAGCAGAGCACAAGGACTTGGTGGATATTCTACTCTCTATCCAAGCGAGGGAGAGAGAGAGCAATGGAGACTCTTCAAACTCTTCAGACTCTTCTTCTTCATCCAATTTCGTTCACTGCTTTCGTCCTTATCCTGATCTTCCTTCTCCAAAAATGGCTGCTTTCCAATCAAAACACATCAAAAGCCTCACCACCATCTCCGCCAAAGCATCCAATCTTCGGCCACCTTTTGAAGCTCGGCCGCCTTCCTCATATCACTCTCCAAAACTATGCTCGCCGCTATGGTCCTCTGTTTCTCCTTCACTTGGGCGCCAAGCCCACCATCGTGGTCTCTTCTCCCCAACTGGCTCGTGA

Coding sequence (CDS)

ATGGAAACTCTTCTTCTTCTTCAATCATCACTTCATCCAATTTTGTTCGGTGGTTGCGTCCTTGTCCTGATCTTCCTTCTCCAAAAATGGCTGCTTTCCAATCAAAACTCATCAAAAGCCTCGCCGCCATCGCCGCCAAAGCATCCAATCTTCGGCCACCTTTTGAAGCTTGGCCGCCTTCCTCATATTACTCTCCAAAACTATGCTCGCCGCTATGGTCCTCTGTTTCTCCTTCACTTGGGCGCCAAGCCCACCATCGTGGTCTCTTCTCCCCAACTGGCTCGTGATATCATGAAGACCCACGACCTTAGCTTTGCCAATCGACCCAAATCAAGCATGGCTGATAAGCTTCTTTATGGGTCAAAAGATATAGCGATGTCTCCTTATGGTGAATATTGGAGGCGGATGAGAAGCATCGGCGTGCTCCGTCTTTTGAGCCACAACAGTGTCCAATCGTTTTGTTGTGTTAGAGAACAAGAAGTGAAGGCCATGATCGACAACATCCAACGCAATCCACTGTCGGTTAATTTCACTGAACTCTTCTCTGGACTGACCAACGACGTGATTTGCAGGGTGGCCTTTGGGAGAAAATATGGGATTGGTCAAGATGGGTCGAAGCTTAGGTCCCTTCTCTTGCAATTTGGGGAGTTAATGGCGAGTTTTATCATTCGAGACTACGTTCCATTGCTGGGTTGGATCGATGGCATTACTGGGTTCGACGCCAAAGCCAAGAGGGTAGCCAAAGAGCTGGACATTTTGTTAGATGAAGTGATTCAAGATCATATTCATTTACAGAACAGAGCAGAGCACAAGGACTTGGTGGATATTCTACTCTCTATCCAAGCGAGGGAGAGAGAGAGCAATGGAGACTCTTCAAACTCTTCAGACTCTTCTTCTTCATCCAATTTCGTTCACTGCTTTCGTCCTTATCCTGATCTTCCTTCTCCAAAAATGGCTGCTTTCCAATCAAAACACATCAAAAGCCTCACCACCATCTCCGCCAAAGCATCCAATCTTCGGCCACCTTTTGAAGCTCGGCCGCCTTCCTCATATCACTCTCCAAAACTATGCTCGCCGCTATGGTCCTCTGTTTCTCCTTCACTTGGGCGCCAAGCCCACCATCGTGGTCTCTTCTCCCCAACTGGCTCGTGA

Protein sequence

METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTELFSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQARERESNGDSSNSSDSSSSSNFVHCFRPYPDLPSPKMAAFQSKHIKSLTTISAKASNLRPPFEARPPSSYHSPKLCSPLWSSVSPSLGRQAHHRGLFSPTGS
Homology
BLAST of Cp4.1LG04g15950 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.1e-68
Identity = 135/282 (47.87%), Postives = 196/282 (69.50%), Query Frame = 0

Query: 9   SSLHPILFGGCVLVLIFLLQKWLLSNQNSSKAS--PPSPPKHPIFGHLLKLGRLPHITLQ 68
           S L P+ F   + + + LL  W  S + +++ S  PPSPPK PI G+L ++G  PH +LQ
Sbjct: 16  SFLQPLAF-TLLAIFLVLLYTWYSSTKTTTQKSTQPPSPPKLPIIGNLHQIGSYPHRSLQ 75

Query: 69  NYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAM 128
             ++R+GPL LLH G+ P +VVSS + AR+I+KTHDL+F++RPKS++ +KLLY  KD+A 
Sbjct: 76  ALSQRHGPLMLLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVAS 135

Query: 129 SPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSV-NFTELFSGLT 188
           +PYGEYWR++RSI VL LLS+  V+SF  VRE+E K+MI NI+ +  SV N +E+F  LT
Sbjct: 136 APYGEYWRQVRSICVLNLLSNRRVRSFRSVREEETKSMIRNIKGSSSSVLNLSEMFVRLT 195

Query: 189 NDVICRVAFGRKYGIGQDGSK---LRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAK 248
           NDV+C+VA GRKY  G+ G      + +L +FG+L+ +  I DYVP L W+  + G  AK
Sbjct: 196 NDVVCKVALGRKYSDGEGGESGRMFKEILGEFGDLLGTVNIGDYVPWLSWLSHVNGLGAK 255

Query: 249 AKRVAKELDILLDEVIQDHIHLQNRA---EHKDLVDILLSIQ 282
             +VAK+LD  +D V+Q+H++  +R+   + KD +DILL+IQ
Sbjct: 256 LDKVAKQLDDFIDTVVQEHMNHSSRSGDDDQKDFLDILLAIQ 296

BLAST of Cp4.1LG04g15950 vs. ExPASy Swiss-Prot
Match: P37119 (Cytochrome P450 71A3 (Fragment) OS=Solanum melongena OX=4111 GN=CYP71A3 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 4.1e-63
Identity = 128/271 (47.23%), Postives = 182/271 (67.16%), Query Frame = 0

Query: 20  VLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYGPLFLLH 79
           + V I  L K   +  N++K  PPSP K PI G+L +LG  PH +L   +++YGP+ LLH
Sbjct: 4   LFVFILFLHKCFFTTSNNNKKLPPSPRKLPIIGNLHQLGLHPHRSLHKLSKKYGPVMLLH 63

Query: 80  LGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYWRRMRSI 139
           LG+KP IV SS +  RDIMKT+DL ++NRPKS MAD+L+YGSKD++ SP+GEYWR++RSI
Sbjct: 64  LGSKPVIVASSVEAVRDIMKTNDLVWSNRPKSKMADRLIYGSKDVSFSPHGEYWRQIRSI 123

Query: 140 GVLRLLSHNSVQSFCCVREQEVKAMIDNIQR----NPLSVNFTELFSGLTNDVICRVAFG 199
            VL LLS+  VQS+   RE+E   MI+ +++    +  + +  +LF  L  ++I RVA G
Sbjct: 124 TVLHLLSNKRVQSYRAAREEETSNMIEKLKQMSNSSSSATDLRDLFCWLAYNIINRVALG 183

Query: 200 RKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDILLD 259
           +KY    D    ++ L +F EL+ +F + DY+P L W++ ITGFD+K  +VAK+LD  L+
Sbjct: 184 KKY---NDEIDAKATLDKFVELLGTFNVGDYIPCLEWVNKITGFDSKVDKVAKDLDTFLE 243

Query: 260 EVIQDHI----HLQNRA-EHKDLVDILLSIQ 282
            VI+ H+      +NRA E KDLVD+LL IQ
Sbjct: 244 FVIEAHMIRNEKEENRAGESKDLVDVLLEIQ 271

BLAST of Cp4.1LG04g15950 vs. ExPASy Swiss-Prot
Match: P37118 (Cytochrome P450 71A2 OS=Solanum melongena OX=4111 GN=CYP71A2 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.5e-60
Identity = 128/272 (47.06%), Postives = 176/272 (64.71%), Query Frame = 0

Query: 20  VLVLIFLL--QKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYGPLFL 79
           + V IFLL    +  +++  +    PSP K PI G+L +LG LPH +L   +++YGP+ L
Sbjct: 14  LFVFIFLLIHHCFFTTSKKQNMLLLPSPRKLPIIGNLHQLGSLPHRSLHKLSQKYGPVML 73

Query: 80  LHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYWRRMR 139
           LH G+KP IV SS   ARDIMKTHD+ +A+RPKSS+ D+L YGSKD+  SP+GEYWRR +
Sbjct: 74  LHFGSKPVIVASSVDAARDIMKTHDVVWASRPKSSIVDRLSYGSKDVGFSPFGEYWRRAK 133

Query: 140 SIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLS--VNFTELFSGLTNDVICRVAFG 199
           SI VL LLS+  VQS+  VR +E   MI  I++   S  +N  E    LTN++I RVA G
Sbjct: 134 SITVLHLLSNTRVQSYRNVRAEETANMIGKIRQGCDSSVINLGEHLCSLTNNIISRVALG 193

Query: 200 RKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDILLD 259
           R Y   + G  +  ++ QF EL+  F + DY+P L W++  TG DAK K+VAKELD+ L+
Sbjct: 194 RTYDEKESG--IEHIIEQFVELLGIFNVGDYIPRLEWVNKFTGLDAKVKKVAKELDMFLE 253

Query: 260 EVIQDHIHLQNRAEH------KDLVDILLSIQ 282
            VI++HI  + + E+      KD VD+LL IQ
Sbjct: 254 IVIEEHIIRKKKEEYTSTGEAKDFVDVLLEIQ 283

BLAST of Cp4.1LG04g15950 vs. ExPASy Swiss-Prot
Match: P37117 (Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.9e-60
Identity = 122/270 (45.19%), Postives = 174/270 (64.44%), Query Frame = 0

Query: 20  VLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYGPLFLLH 79
           +L +  L+     +++  +   PPSP K PI G+L +LG  PH +L+  +++YGP+ LLH
Sbjct: 15  LLFIFLLIHHCFTTSKTQNMFLPPSPRKLPIIGNLHQLGSHPHRSLRKLSQKYGPVMLLH 74

Query: 80  LGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYWRRMRSI 139
           LG+KP IV SS   ARDI+KTHD  +A RPK S+AD LLYGSKD+  SP+GEYW ++RSI
Sbjct: 75  LGSKPVIVASSVDAARDILKTHDHVWATRPKYSIADSLLYGSKDVGFSPFGEYWWQVRSI 134

Query: 140 GVLRLLSHNSVQSFCCVREQEVKAMIDNIQR--NPLSVNFTELFSGLTNDVICRVAFGRK 199
            VL LLS+  VQS+  VRE+E   MI+ I++  +   +N  E    LTN++  RVA GR 
Sbjct: 135 VVLHLLSNKRVQSYRDVREEETANMIEKIRQGCDASVINLGEHLCFLTNNITSRVALGRT 194

Query: 200 YGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDILLDEV 259
           Y   + G   + +L QF +L+ +F + DY+P L W++ ITG D K +++AK+LD  LD V
Sbjct: 195 YDERESGIDAKDILEQFLQLLDTFNVGDYIPWLKWVNKITGLDTKVEKIAKKLDTFLDSV 254

Query: 260 IQDHIHLQNRAEH------KDLVDILLSIQ 282
           I++HI    + E+      KD VD+LL IQ
Sbjct: 255 IEEHIIRNKKEEYAITDEAKDFVDVLLEIQ 284

BLAST of Cp4.1LG04g15950 vs. ExPASy Swiss-Prot
Match: Q9STL2 (Cytochrome P450 71A21 OS=Arabidopsis thaliana OX=3702 GN=CYP71A21 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.5e-60
Identity = 126/280 (45.00%), Postives = 178/280 (63.57%), Query Frame = 0

Query: 3   TLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPH 62
           T+++LQS    I+F   + +L F  QK     +     +P SPP+ P+ G+L +LG  PH
Sbjct: 5   TMIILQSL---IIF---ITILFFKKQK-----RGKKSNTPRSPPRLPLIGNLHQLGHHPH 64

Query: 63  ITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSK 122
            +L + + RYGPL LLHLG  P +VVSS  +ARDI+KTHD  FA+RP+S + +KL Y  +
Sbjct: 65  RSLCSLSHRYGPLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGR 124

Query: 123 DIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQR-NPLSVNFTELF 182
           D+A +PYGEYWR+++S+ VLRLLS+  V SF  VR++E+  M++ IQ+ + L VN +EL 
Sbjct: 125 DVAFAPYGEYWRQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQVNVSELL 184

Query: 183 SGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDA 242
             LTNDVI R+A GRKY    D  +L   L+    LM  F +  YVP LGWID I+G D 
Sbjct: 185 GSLTNDVISRIALGRKYSGETDSKELMKRLMM---LMGEFSVGTYVPWLGWIDWISGLDG 244

Query: 243 KAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ 282
           +  +   +LD  L++V+QDH+      +  D VD+LL IQ
Sbjct: 245 QLNKTGNDLDEFLEKVVQDHV--DGDGQRTDFVDVLLRIQ 268

BLAST of Cp4.1LG04g15950 vs. NCBI nr
Match: XP_023530748.1 (cytochrome P450 71A6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 561 bits (1447), Expect = 1.91e-196
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL
Sbjct: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG
Sbjct: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL
Sbjct: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD
Sbjct: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ 281
           AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ
Sbjct: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ 281

BLAST of Cp4.1LG04g15950 vs. NCBI nr
Match: KAG6587439.1 (Cytochrome P450 71A3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 545 bits (1403), Expect = 4.09e-193
Identity = 272/282 (96.45%), Postives = 277/282 (98.23%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           METLLLLQSSLH ILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL
Sbjct: 1   METLLLLQSSLHTILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDL+FANRPKSSMADKLLYG
Sbjct: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLTFANRPKSSMADKLLYG 120

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSF CVREQEVKAMID+IQRNPLSVN TEL
Sbjct: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFRCVREQEVKAMIDSIQRNPLSVNLTEL 180

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD
Sbjct: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQA 282
            KAKRVAKELD+LLDEVI+DHIHLQNRAEHKDLVD+LL IQA
Sbjct: 241 VKAKRVAKELDVLLDEVIEDHIHLQNRAEHKDLVDVLLCIQA 282

BLAST of Cp4.1LG04g15950 vs. NCBI nr
Match: XP_022933687.1 (cytochrome P450 71A21-like [Cucurbita moschata])

HSP 1 Score: 536 bits (1382), Expect = 1.04e-186
Identity = 267/282 (94.68%), Postives = 274/282 (97.16%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           METLLLL SSLH ILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL
Sbjct: 1   METLLLLPSSLHTILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDI+KTHDL+FANRPKSSMADKLLYG
Sbjct: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDILKTHDLTFANRPKSSMADKLLYG 120

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWRRMRSIGV+RLLSHNSVQSF CVREQEVKAMIDNIQ NP+SVN TEL
Sbjct: 121 SKDIAMSPYGEYWRRMRSIGVVRLLSHNSVQSFRCVREQEVKAMIDNIQHNPISVNLTEL 180

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FSGLTNDVICRVAFGRKYGIGQDGS LR+LLLQFGELMASFIIRDYVPLLGWIDGITGFD
Sbjct: 181 FSGLTNDVICRVAFGRKYGIGQDGSNLRALLLQFGELMASFIIRDYVPLLGWIDGITGFD 240

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQA 282
            KAKRVAKELD+LLDEVI+DHIHLQNRAEHKDLVDILL IQA
Sbjct: 241 VKAKRVAKELDVLLDEVIEDHIHLQNRAEHKDLVDILLCIQA 282

BLAST of Cp4.1LG04g15950 vs. NCBI nr
Match: XP_022973389.1 (cytochrome P450 71A3-like [Cucurbita maxima])

HSP 1 Score: 519 bits (1337), Expect = 7.06e-180
Identity = 261/282 (92.55%), Postives = 270/282 (95.74%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           METLLL QS LHPILFGGCVLVLIFLL KWLLSNQN+SKASPPSPPK PIFGHLLKLGRL
Sbjct: 1   METLLL-QSCLHPILFGGCVLVLIFLLHKWLLSNQNTSKASPPSPPKLPIFGHLLKLGRL 60

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           PHITLQNYARRYGPLFLLHLG KPTIVVSSPQLARDIMKTHDL+FANRPKSSMADKLLYG
Sbjct: 61  PHITLQNYARRYGPLFLLHLGTKPTIVVSSPQLARDIMKTHDLTFANRPKSSMADKLLYG 120

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSF CVREQEVKAMIDNIQ NP+SVN TEL
Sbjct: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFRCVREQEVKAMIDNIQHNPISVNLTEL 180

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FS LTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGE +ASFIIRDYVPLLGWIDG++GFD
Sbjct: 181 FSRLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGESLASFIIRDYVPLLGWIDGVSGFD 240

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQA 282
            KAKRVAKELD+LLDEVI+DHIHLQNRAE KDL+DILLSIQA
Sbjct: 241 VKAKRVAKELDVLLDEVIEDHIHLQNRAELKDLMDILLSIQA 281

BLAST of Cp4.1LG04g15950 vs. NCBI nr
Match: XP_022933473.1 (cytochrome P450 71A3-like [Cucurbita moschata])

HSP 1 Score: 463 bits (1192), Expect = 7.41e-158
Identity = 233/282 (82.62%), Postives = 251/282 (89.01%), Query Frame = 0

Query: 1   METLLLLQSSL-HPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGR 60
           METL  LQ+ L HPI     VL+LIFLLQKWLLSNQN+SKASPPSPPKHPIFGHLLKLGR
Sbjct: 1   METLQTLQTLLLHPISCTAFVLILIFLLQKWLLSNQNTSKASPPSPPKHPIFGHLLKLGR 60

Query: 61  LPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLY 120
           LPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDL FANRPKSS+ADKLLY
Sbjct: 61  LPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLIFANRPKSSVADKLLY 120

Query: 121 GSKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTE 180
           GSKDI+MSPYGEYWR++RSIGVL LLS+N VQSF CVREQEVKAMID IQ NPLSVN TE
Sbjct: 121 GSKDISMSPYGEYWRQIRSIGVLHLLSNNRVQSFRCVREQEVKAMIDYIQHNPLSVNLTE 180

Query: 181 LFSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGF 240
           LFS LTNDVICRVA GRKYGIG+DG+K RSLLLQFGE MASF IRD++P LGWIDGI+G 
Sbjct: 181 LFSRLTNDVICRVALGRKYGIGEDGTKFRSLLLQFGESMASFSIRDFIPWLGWIDGISGL 240

Query: 241 DAKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ 281
           D +AKRVAKELD+ LD VIQDH+H +NR EH DLVD LL I+
Sbjct: 241 DVQAKRVAKELDVFLDRVIQDHVHPENRDEHNDLVDTLLCIK 282

BLAST of Cp4.1LG04g15950 vs. ExPASy TrEMBL
Match: A0A6J1F0I2 (cytochrome P450 71A21-like OS=Cucurbita moschata OX=3662 GN=LOC111441025 PE=3 SV=1)

HSP 1 Score: 536 bits (1382), Expect = 5.03e-187
Identity = 267/282 (94.68%), Postives = 274/282 (97.16%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           METLLLL SSLH ILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL
Sbjct: 1   METLLLLPSSLHTILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDI+KTHDL+FANRPKSSMADKLLYG
Sbjct: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDILKTHDLTFANRPKSSMADKLLYG 120

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWRRMRSIGV+RLLSHNSVQSF CVREQEVKAMIDNIQ NP+SVN TEL
Sbjct: 121 SKDIAMSPYGEYWRRMRSIGVVRLLSHNSVQSFRCVREQEVKAMIDNIQHNPISVNLTEL 180

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FSGLTNDVICRVAFGRKYGIGQDGS LR+LLLQFGELMASFIIRDYVPLLGWIDGITGFD
Sbjct: 181 FSGLTNDVICRVAFGRKYGIGQDGSNLRALLLQFGELMASFIIRDYVPLLGWIDGITGFD 240

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQA 282
            KAKRVAKELD+LLDEVI+DHIHLQNRAEHKDLVDILL IQA
Sbjct: 241 VKAKRVAKELDVLLDEVIEDHIHLQNRAEHKDLVDILLCIQA 282

BLAST of Cp4.1LG04g15950 vs. ExPASy TrEMBL
Match: A0A6J1IEF5 (cytochrome P450 71A3-like OS=Cucurbita maxima OX=3661 GN=LOC111471939 PE=3 SV=1)

HSP 1 Score: 519 bits (1337), Expect = 3.42e-180
Identity = 261/282 (92.55%), Postives = 270/282 (95.74%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           METLLL QS LHPILFGGCVLVLIFLL KWLLSNQN+SKASPPSPPK PIFGHLLKLGRL
Sbjct: 1   METLLL-QSCLHPILFGGCVLVLIFLLHKWLLSNQNTSKASPPSPPKLPIFGHLLKLGRL 60

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           PHITLQNYARRYGPLFLLHLG KPTIVVSSPQLARDIMKTHDL+FANRPKSSMADKLLYG
Sbjct: 61  PHITLQNYARRYGPLFLLHLGTKPTIVVSSPQLARDIMKTHDLTFANRPKSSMADKLLYG 120

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSF CVREQEVKAMIDNIQ NP+SVN TEL
Sbjct: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFRCVREQEVKAMIDNIQHNPISVNLTEL 180

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FS LTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGE +ASFIIRDYVPLLGWIDG++GFD
Sbjct: 181 FSRLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGESLASFIIRDYVPLLGWIDGVSGFD 240

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQA 282
            KAKRVAKELD+LLDEVI+DHIHLQNRAE KDL+DILLSIQA
Sbjct: 241 VKAKRVAKELDVLLDEVIEDHIHLQNRAELKDLMDILLSIQA 281

BLAST of Cp4.1LG04g15950 vs. ExPASy TrEMBL
Match: A0A6J1F4U4 (cytochrome P450 71A3-like OS=Cucurbita moschata OX=3662 GN=LOC111440879 PE=3 SV=1)

HSP 1 Score: 463 bits (1192), Expect = 3.59e-158
Identity = 233/282 (82.62%), Postives = 251/282 (89.01%), Query Frame = 0

Query: 1   METLLLLQSSL-HPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGR 60
           METL  LQ+ L HPI     VL+LIFLLQKWLLSNQN+SKASPPSPPKHPIFGHLLKLGR
Sbjct: 1   METLQTLQTLLLHPISCTAFVLILIFLLQKWLLSNQNTSKASPPSPPKHPIFGHLLKLGR 60

Query: 61  LPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLY 120
           LPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDL FANRPKSS+ADKLLY
Sbjct: 61  LPHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLIFANRPKSSVADKLLY 120

Query: 121 GSKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTE 180
           GSKDI+MSPYGEYWR++RSIGVL LLS+N VQSF CVREQEVKAMID IQ NPLSVN TE
Sbjct: 121 GSKDISMSPYGEYWRQIRSIGVLHLLSNNRVQSFRCVREQEVKAMIDYIQHNPLSVNLTE 180

Query: 181 LFSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGF 240
           LFS LTNDVICRVA GRKYGIG+DG+K RSLLLQFGE MASF IRD++P LGWIDGI+G 
Sbjct: 181 LFSRLTNDVICRVALGRKYGIGEDGTKFRSLLLQFGESMASFSIRDFIPWLGWIDGISGL 240

Query: 241 DAKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ 281
           D +AKRVAKELD+ LD VIQDH+H +NR EH DLVD LL I+
Sbjct: 241 DVQAKRVAKELDVFLDRVIQDHVHPENRDEHNDLVDTLLCIK 282

BLAST of Cp4.1LG04g15950 vs. ExPASy TrEMBL
Match: A0A6J1KQX1 (cytochrome P450 71A22-like OS=Cucurbita maxima OX=3661 GN=LOC111497450 PE=3 SV=1)

HSP 1 Score: 448 bits (1153), Expect = 7.06e-155
Identity = 228/284 (80.28%), Postives = 246/284 (86.62%), Query Frame = 0

Query: 1   METLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRL 60
           ++TLLLL     PI F   VL+LIFLLQKWLLSNQN+SKASPPSPPK PI GHLL LGRL
Sbjct: 4   LQTLLLL-----PISFTAFVLILIFLLQKWLLSNQNTSKASPPSPPKLPIVGHLLNLGRL 63

Query: 61  PHITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYG 120
           P ITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDL FANRPKSS+ADKLLYG
Sbjct: 64  PRITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLIFANRPKSSVADKLLYG 123

Query: 121 SKDIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTEL 180
           SKDIAMSPYGEYWR+MRSIGVL LLS+N VQSF  VREQEVKAMIDNIQ NPLSVN TEL
Sbjct: 124 SKDIAMSPYGEYWRQMRSIGVLHLLSNNRVQSFRYVREQEVKAMIDNIQHNPLSVNLTEL 183

Query: 181 FSGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFD 240
           FS LTNDVICRVA GRKYGIG+DG+K RSLLLQFGE MASF IRD++P LGWIDGI+G D
Sbjct: 184 FSRLTNDVICRVALGRKYGIGEDGTKFRSLLLQFGESMASFSIRDFIPWLGWIDGISGLD 243

Query: 241 AKAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQARE 284
            +AKR AKELD+ LD VIQDH+H +NR EH DLVD LL I+  +
Sbjct: 244 VQAKRAAKELDVFLDRVIQDHVHPENRDEHNDLVDTLLCIKTED 282

BLAST of Cp4.1LG04g15950 vs. ExPASy TrEMBL
Match: A0A6J1JG96 (cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1)

HSP 1 Score: 371 bits (952), Expect = 6.84e-122
Identity = 185/275 (67.27%), Postives = 225/275 (81.82%), Query Frame = 0

Query: 12  HPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPP-KHPIFGHLLKLGRLPHITLQNYAR 71
           HP+   G +++ I+LL +   S+Q   ++SPPSPP K PIFGHLLKLG  PHITLQ+YAR
Sbjct: 12  HPVSVAGFIVLFIWLLFRQKSSSQEE-QSSPPSPPTKLPIFGHLLKLGSHPHITLQSYAR 71

Query: 72  RYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYG 131
           R+GPLFLLHLG+KPTIVVSS ++ARD+MKTHDL FANRPKSS++DKLLY + DI+ +PYG
Sbjct: 72  RHGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYG 131

Query: 132 EYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQRNPLSVNFTELFSGLTNDVIC 191
           EYWR+MRS+GVL LLS+  VQSF  VRE+EVK MI+ IQRNP+SVN TE FSGLTN+V+C
Sbjct: 132 EYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTETFSGLTNNVVC 191

Query: 192 RVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKEL 251
           RVA G KYG+G+D  KLRSLLL+F EL+ SF +RD++P LGWIDGI+G DAKAKRVAKEL
Sbjct: 192 RVALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKEL 251

Query: 252 DILLDEVIQDHIHLQNRAE----HKDLVDILLSIQ 281
           D+ LD VIQDH+  +NR E    H DLVD+LL IQ
Sbjct: 252 DVFLDRVIQDHVDSENRDENSDKHMDLVDVLLWIQ 285

BLAST of Cp4.1LG04g15950 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 233.0 bits (593), Expect = 3.9e-61
Identity = 126/280 (45.00%), Postives = 178/280 (63.57%), Query Frame = 0

Query: 3   TLLLLQSSLHPILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPH 62
           T+++LQS    I+F   + +L F  QK     +     +P SPP+ P+ G+L +LG  PH
Sbjct: 5   TMIILQSL---IIF---ITILFFKKQK-----RGKKSNTPRSPPRLPLIGNLHQLGHHPH 64

Query: 63  ITLQNYARRYGPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSK 122
            +L + + RYGPL LLHLG  P +VVSS  +ARDI+KTHD  FA+RP+S + +KL Y  +
Sbjct: 65  RSLCSLSHRYGPLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGR 124

Query: 123 DIAMSPYGEYWRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQR-NPLSVNFTELF 182
           D+A +PYGEYWR+++S+ VLRLLS+  V SF  VR++E+  M++ IQ+ + L VN +EL 
Sbjct: 125 DVAFAPYGEYWRQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQVNVSELL 184

Query: 183 SGLTNDVICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDA 242
             LTNDVI R+A GRKY    D  +L   L+    LM  F +  YVP LGWID I+G D 
Sbjct: 185 GSLTNDVISRIALGRKYSGETDSKELMKRLMM---LMGEFSVGTYVPWLGWIDWISGLDG 244

Query: 243 KAKRVAKELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQ 282
           +  +   +LD  L++V+QDH+      +  D VD+LL IQ
Sbjct: 245 QLNKTGNDLDEFLEKVVQDHV--DGDGQRTDFVDVLLRIQ 268

BLAST of Cp4.1LG04g15950 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 232.6 bits (592), Expect = 5.1e-61
Identity = 121/269 (44.98%), Postives = 178/269 (66.17%), Query Frame = 0

Query: 14  ILFGGCVLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYG 73
           IL    + + I  L+K L   +     +PPSPP  P+ G+L +LGR  H +L + +RRYG
Sbjct: 6   ILLWSIIFMTILFLKKQLSGKKGK---TPPSPPGLPLIGNLHQLGRHTHRSLCDLSRRYG 65

Query: 74  PLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYW 133
           PL LLHLG  P ++VSS  +A++I+KTHD +FANRP+S ++ KLLY ++D+A +PYGEYW
Sbjct: 66  PLMLLHLGRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYW 125

Query: 134 RRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQR-NPLSVNFTELFSGLTNDVICRV 193
           R+M+S+ V+ LLS+  V+SF  VRE+E+  M+  I++ + L  N +++   LTNDVICRV
Sbjct: 126 RQMKSVCVIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLTNDVICRV 185

Query: 194 AFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDI 253
           A GRKYG   D  KL   L    EL+ +F I  +VP L W+D I G+DA+  ++ K+LD 
Sbjct: 186 ALGRKYGGETDFKKLTDRL---SELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDD 245

Query: 254 LLDEVIQDHIHLQNRAEHKDLVDILLSIQ 282
             ++V+QDH    +R +  DL+D LL ++
Sbjct: 246 FFEKVVQDH-EDGDRRDGTDLIDALLRVK 267

BLAST of Cp4.1LG04g15950 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 230.3 bits (586), Expect = 2.5e-60
Identity = 117/263 (44.49%), Postives = 172/263 (65.40%), Query Frame = 0

Query: 20  VLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYGPLFLLH 79
           V ++IF  QK     +   + + PSPP  P+ G+L +LGR PH +L + + RYGPL LLH
Sbjct: 15  VTIIIFRKQK-----RGKKRNTLPSPPGLPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLH 74

Query: 80  LGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYWRRMRSI 139
            G  P +VVSS +LARD++KTHD  FA+RP+S + +KLLY   D+A +PYGEYWR+M+S+
Sbjct: 75  FGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSV 134

Query: 140 GVLRLLSHNSVQSFCCVREQEVKAMIDNIQRN-PLSVNFTELFSGLTNDVICRVAFGRKY 199
            VL L S+  V+SF  VRE+E+  M++ I+++  L VN +++   LTNDVIC+VA GRKY
Sbjct: 135 CVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSLTNDVICKVALGRKY 194

Query: 200 GIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDILLDEVI 259
           G   D    + L+ +  +L+ +F +  YVP L WID I G D + ++ A ++D   + V+
Sbjct: 195 GGETD---FKELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVV 254

Query: 260 QDHIHLQNRAEHKDLVDILLSIQ 282
           QDH+      +  D VD+LL+IQ
Sbjct: 255 QDHV--DGNRDMTDFVDVLLAIQ 267

BLAST of Cp4.1LG04g15950 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 229.2 bits (583), Expect = 5.7e-60
Identity = 116/263 (44.11%), Postives = 172/263 (65.40%), Query Frame = 0

Query: 20  VLVLIFLLQKWLLSNQNSSKASPPSPPKHPIFGHLLKLGRLPHITLQNYARRYGPLFLLH 79
           + +L F+ QK     +     +P SPP+ P+ G+L +LGR PH +L + + RYGPL LL 
Sbjct: 16  ITILFFIKQK-----KGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRYGPLMLLR 75

Query: 80  LGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEYWRRMRSI 139
            G  P +VVSS  +ARDI+KT+D  FA+RP+S + +K+ Y ++D+A++PYGEYWR+M+S+
Sbjct: 76  FGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQMKSV 135

Query: 140 GVLRLLSHNSVQSFCCVREQEVKAMIDNIQR-NPLSVNFTELFSGLTNDVICRVAFGRKY 199
            VL LL++  V+SF  VR++E+  M++ IQ+ + L VN +EL   LTNDVI RVA GRKY
Sbjct: 136 CVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQVNLSELLGSLTNDVISRVALGRKY 195

Query: 200 GIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVAKELDILLDEVI 259
               D +  + L+ +  +L+  F +  YVP L WID I+G D + K+   +LD  L++V+
Sbjct: 196 ---SDETDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEKVV 255

Query: 260 QDHIHLQNRAEHKDLVDILLSIQ 282
           QD  H    A+  D VD+LL IQ
Sbjct: 256 QD--HEDGDAQRTDFVDVLLRIQ 268

BLAST of Cp4.1LG04g15950 vs. TAIR 10
Match: AT4G13310.2 (cytochrome P450, family 71, subfamily A, polypeptide 20 )

HSP 1 Score: 226.5 bits (576), Expect = 3.7e-59
Identity = 115/275 (41.82%), Postives = 175/275 (63.64%), Query Frame = 0

Query: 14  ILFGGCVLVLIFLLQKWLLSNQNSSKAS-PPSPPKHPIFGHLLKLGRLPHITLQNYARRY 73
           IL   C+  L+ LL K +L    +   + PPSP + P+ G+L +L    H +L++ + RY
Sbjct: 4   ILITLCLTTLLALLLKSILKRTATKNFNLPPSPWRLPVIGNLHQLSLHTHRSLRSLSLRY 63

Query: 74  GPLFLLHLGAKPTIVVSSPQLARDIMKTHDLSFANRPKSSMADKLLYGSKDIAMSPYGEY 133
           GPL LLH G  P ++VSS  +A D+MKTHDL  ANRPK+ + DK+L G +D+A +PYGEY
Sbjct: 64  GPLMLLHFGRTPVLIVSSADVAHDVMKTHDLVCANRPKTKVVDKILSGGRDVAFAPYGEY 123

Query: 134 WRRMRSIGVLRLLSHNSVQSFCCVREQEVKAMIDNIQR-----NPLSVNFTELFSGLTND 193
           WR+M+SI +  LL++  V+S+  +RE+E+K MI+ +++     +P  VN +++   LTND
Sbjct: 124 WRQMKSICIQNLLNNKMVRSYEKIREEEIKRMIEKLEKASCSSSPSPVNLSQILMTLTND 183

Query: 194 VICRVAFGRKYGIGQDGSKLRSLLLQFGELMASFIIRDYVPLLGWIDGITGFDAKAKRVA 253
           +ICRVA GRKY   +DG  + +++  F  L+  F + +Y+P L WID I G D K + V 
Sbjct: 184 IICRVALGRKYSGKKDGIDVENIVRTFAALLGEFPVGEYIPSLSWIDRIRGLDHKMEVVD 243

Query: 254 KELDILLDEVIQDHIHLQNRAEHKDLVDILLSIQA 283
           K  D  L+ V+++H    ++    DLVD LL+IQ+
Sbjct: 244 KRFDEFLERVVKEH-EEADKETRSDLVDKLLTIQS 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A068Q6L21.1e-6847.87Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
P371194.1e-6347.23Cytochrome P450 71A3 (Fragment) OS=Solanum melongena OX=4111 GN=CYP71A3 PE=2 SV=... [more]
P371181.5e-6047.06Cytochrome P450 71A2 OS=Solanum melongena OX=4111 GN=CYP71A2 PE=2 SV=1[more]
P371171.9e-6045.19Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1[more]
Q9STL25.5e-6045.00Cytochrome P450 71A21 OS=Arabidopsis thaliana OX=3702 GN=CYP71A21 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023530748.11.91e-196100.00cytochrome P450 71A6-like [Cucurbita pepo subsp. pepo][more]
KAG6587439.14.09e-19396.45Cytochrome P450 71A3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933687.11.04e-18694.68cytochrome P450 71A21-like [Cucurbita moschata][more]
XP_022973389.17.06e-18092.55cytochrome P450 71A3-like [Cucurbita maxima][more]
XP_022933473.17.41e-15882.62cytochrome P450 71A3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1F0I25.03e-18794.68cytochrome P450 71A21-like OS=Cucurbita moschata OX=3662 GN=LOC111441025 PE=3 SV... [more]
A0A6J1IEF53.42e-18092.55cytochrome P450 71A3-like OS=Cucurbita maxima OX=3661 GN=LOC111471939 PE=3 SV=1[more]
A0A6J1F4U43.59e-15882.62cytochrome P450 71A3-like OS=Cucurbita moschata OX=3662 GN=LOC111440879 PE=3 SV=... [more]
A0A6J1KQX17.06e-15580.28cytochrome P450 71A22-like OS=Cucurbita maxima OX=3661 GN=LOC111497450 PE=3 SV=1[more]
A0A6J1JG966.84e-12267.27cytochrome P450 71A25-like OS=Cucurbita maxima OX=3661 GN=LOC111484850 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48320.13.9e-6145.00cytochrome P450, family 71, subfamily A, polypeptide 21 [more]
AT3G48280.15.1e-6144.98cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
AT3G48270.12.5e-6044.49cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
AT3G48310.15.7e-6044.11cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
AT4G13310.23.7e-5941.82cytochrome P450, family 71, subfamily A, polypeptide 20 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 69..88
score: 37.42
coord: 93..114
score: 19.65
coord: 184..202
score: 24.42
IPR001128Cytochrome P450PFAMPF00067p450coord: 42..290
e-value: 2.0E-29
score: 102.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..303
e-value: 8.4E-51
score: 175.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 42..288
NoneNo IPR availablePANTHERPTHR47955CYTOCHROME P450 FAMILY 71 PROTEINcoord: 21..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g15950.1Cp4.1LG04g15950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen