Cp4.1LG04g14290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g14290 vs. ExPASy Swiss-Prot
Match: Q61749 (Translation initiation factor eIF-2B subunit delta OS=Mus musculus OX=10090 GN=Eif2b4 PE=1 SV=2)

HSP 1 Score: 333.6 bits (854), Expect = 5.1e-90
Identity = 227/567 (40.04%), Postives = 308/567 (54.32%), Query Frame = 0

Query: 85  VRVGSHNPSEMLLRSSPMASSSTR--IAGEELEFSEVSSASVLLRRSGSGKLASSCPGVG 144
           V V   + SEM    SP   ++ R     E+L+  +        R+   G        V 
Sbjct: 6   VAVREESRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQEIGSAVS 65

Query: 145 FYMAAKRGDNLKTMPANSVRTLGTNKPAGATEKMGGASTEAKDHLPSSSKPLKEKTTKAE 204
              AA+R D ++ +P    +  GT     A EK+    ++A+               +AE
Sbjct: 66  ---AAQRQDPIRELPGPGSQLGGT-----AGEKLPAGRSKAE--------------LRAE 125

Query: 205 RRALQEAQRAAKVSAKGEANKSGTASGATAGKVGKQPSTVKGPATPSVASADNRGGDRIV 264
           RRA QEA+RA K + KGE               G  P      A PS A     G  R+ 
Sbjct: 126 RRAKQEAERALKQARKGEQG-------------GVPPQ-----ACPSTAGETTSGVKRV- 185

Query: 265 DKERKKDAPPPRMQFDDKHRVERAKKRAVFNQTEAR----NRVELFRHLPQYEQGTQLPD 324
                    P     DD   + R  ++    Q   R    ++V LF HLPQY + + L  
Sbjct: 186 ---------PEHTPADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQ 245

Query: 325 LVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDL 384
            +S       IHPA+ ++GLQY  G ISG NARCIA+LHA QQ I+DY+TPP + L RDL
Sbjct: 246 YMS--IPSSVIHPAMVRLGLQYSQGLISGSNARCIALLHALQQVIQDYTTPPSEELSRDL 305

Query: 385 TAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLSLDISRFINEKI 444
             K+  Y+SF  +CRP+S SM NAI+FL   +  +  S  E EAK+ L   + R++ EKI
Sbjct: 306 VNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALDRYVQEKI 365

Query: 445 MLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQAL 504
           +LA + I R A+TKI DGDV+L YG   +V  +L  A   G++FRVVVVDSRP+LEG+ +
Sbjct: 366 VLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHM 425

Query: 505 LRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVLANGTVYSRVGTAGIAMVADAFHV 564
           L  L+  G+  +Y  I A SYV+ EV +V LGA A+LANG+V SRVGTA +A+VA A +V
Sbjct: 426 LHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNV 485

Query: 565 PVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISYLDSLTSKEHLQLLNLMYD 624
           PVL CCE YKF ERVQ D+   NEL DPD +     R D   L +  S   L+LLNL+YD
Sbjct: 486 PVLVCCETYKFCERVQTDAFVSNELDDPDDLQ--CKRGDQVALANWQSHPSLRLLNLVYD 518

Query: 625 ATPSDYISMIVTDYGMIPPTSVPVIVR 646
            TP + + +++T+ GMIP +SVPV++R
Sbjct: 546 VTPPELVDLVITELGMIPCSSVPVVLR 518

BLAST of Cp4.1LG04g14290 vs. ExPASy Swiss-Prot
Match: Q63186 (Translation initiation factor eIF-2B subunit delta OS=Rattus norvegicus OX=10116 GN=Eif2b4 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 5.1e-90
Identity = 207/479 (43.22%), Postives = 278/479 (58.04%), Query Frame = 0

Query: 171 GATEKMGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGA 230
           G   ++GG + E    LP+      +   +AERRA QEA+RA K + KGE          
Sbjct: 78  GTGSQLGGTTGE---KLPAGR---SKAELRAERRAKQEAERALKQARKGEQG-------- 137

Query: 231 TAGKVGKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRA 290
                G  P      A PS A     G  R+          P   Q DD   + R  ++ 
Sbjct: 138 -----GPSPQ-----ACPSTAGEATSGVKRV----------PEHTQADDPTLLRRLLRKP 197

Query: 291 VFNQTEAR----NRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDIS 350
              Q   R    ++V LF HLPQY + + L   +S       IHPA+ ++GLQY  G +S
Sbjct: 198 DRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS--IPSSVIHPAMVRLGLQYSQGLVS 257

Query: 351 GGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFL 410
           G NARCIA+LHA QQ I+DY+TPP + L RDL  K+  Y+SF  +CRP+S SM NAI+F 
Sbjct: 258 GSNARCIALLHALQQVIQDYTTPPNEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFF 317

Query: 411 KNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPC 470
              +  +  S  E EAK+ L   I R++ EKI+LA + I R A+ KI DGDV+L YG   
Sbjct: 318 NKEVTGMSSSKREEEAKSELKEAIDRYVQEKIVLASQAISRFASKKISDGDVILVYGCSS 377

Query: 471 VVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDR 530
           +V  +L  A   G++FRVVVVDSRP+LEG+ +L  L+  G+  +Y  I A SYV+ EV +
Sbjct: 378 LVSRILQEAWVEGRRFRVVVVDSRPRLEGRHMLHCLVRAGVPTSYLLIPAASYVLPEVSK 437

Query: 531 VFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDP 590
           V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+   NEL DP
Sbjct: 438 VLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDP 497

Query: 591 DAISKISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVR 646
           D +     R D   L +  +   L+LLNL+YD TP + + +++T+ GMIP +SVPV++R
Sbjct: 498 DDLQ--CKRGDQVTLANWQNNSSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLR 518

BLAST of Cp4.1LG04g14290 vs. ExPASy Swiss-Prot
Match: P41111 (Translation initiation factor eIF-2B subunit delta OS=Oryctolagus cuniculus OX=9986 GN=EIF2B4 PE=2 SV=2)

HSP 1 Score: 332.8 bits (852), Expect = 8.8e-90
Identity = 210/498 (42.17%), Postives = 293/498 (58.84%), Query Frame = 0

Query: 148 KRGDNLKTMPANSVRTLGTNKPAGATEKMGGASTEAKDHLPSSSKPLKEKTTKAERRALQ 207
           ++G ++ T  A++V       PA      G  S+   + +P+      +   +AERRA Q
Sbjct: 53  EKGTDVDT--ASAVSAAQCPGPAREAPGPGSQSSTPGEKVPAGR---TKAELRAERRAKQ 112

Query: 208 EAQRAAKVSAKGEANKSGTASGATAGKVGKQPSTVKGPATPSVASADNRGGDRIVDKERK 267
           EA+RA K + KGE               G  P      A+PS A     GG R+ +  + 
Sbjct: 113 EAERALKQARKGEQG-------------GPPPQ-----ASPSTAGEAPAGGKRLTEHTQA 172

Query: 268 KDAPPPRMQFDDKHRVERAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLD 327
            D   P +    +  V +++++ V  + +  ++V LF HLPQY +   L   +S      
Sbjct: 173 DD---PTLL---RRLVRKSERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQYMS--IPSS 232

Query: 328 TIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVS 387
            IHPA+ ++GLQY  G ISG NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+ 
Sbjct: 233 VIHPAMVRLGLQYSQGLISGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYIC 292

Query: 388 FFNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVR 447
           F  +CRPLS SM NAI+FL   I  +  +  E EAKA L     R++ EKI+LA + I+R
Sbjct: 293 FLTQCRPLSASMYNAIKFLNKEITGVSSTKREEEAKAELQAAADRYVQEKIVLAAQAILR 352

Query: 448 HAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGL 507
            A+ KI +GDV+L YG   +V  +L  A   G++FRVVVVDSRP+LEG+ +LR L+  G+
Sbjct: 353 FASKKISNGDVILVYGCSSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRHMLRFLVRAGV 412

Query: 508 SCTYTHINAISYVMREVDRVFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAY 567
             +Y  I A SYV+ EV +V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE Y
Sbjct: 413 PASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETY 472

Query: 568 KFHERVQLDSICFNELGDPDAISKISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISM 627
           KF ERVQ D+   NEL DPD +     R D   L +  S   L+LLNL+YD TP + + +
Sbjct: 473 KFCERVQTDAFVSNELDDPDDLQ--CERGDHVALANWQSHPSLRLLNLVYDVTPPELVDL 517

Query: 628 IVTDYGMIPPTSVPVIVR 646
           ++T+ GMIP +SVPV++R
Sbjct: 533 VITELGMIPCSSVPVVLR 517

BLAST of Cp4.1LG04g14290 vs. ExPASy Swiss-Prot
Match: Q9UI10 (Translation initiation factor eIF-2B subunit delta OS=Homo sapiens OX=9606 GN=EIF2B4 PE=1 SV=2)

HSP 1 Score: 324.7 bits (831), Expect = 2.4e-87
Identity = 222/546 (40.66%), Postives = 303/546 (55.49%), Query Frame = 0

Query: 119 VSSASVLLRR-SGSGKLASSCPGVGFY-MAAKRGDNLKTMPANSVRTLGTNKPAGATEKM 178
           +++ +V +R  SGSG  A   PG G       + + L+       +     +  GA  + 
Sbjct: 1   MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPET 60

Query: 179 GGASTEAK------DHLPSSSKPL---KEKT--------TKAERRALQEAQRAAKVSAKG 238
           G A + A+        LP S   L   +EK          +AERRA QEA+RA K + KG
Sbjct: 61  GSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKG 120

Query: 239 EANKSGTASGATAGKVGKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDD 298
           E  + G    A+    G+ PS VK    P     D+    R+V K  ++  P        
Sbjct: 121 E--QGGPPPKASPSTAGETPSGVK--RLPEYPQVDDLLLRRLVKKPERQQVP-------- 180

Query: 299 KHRVERAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQ 358
                         + +  ++V LF HLPQY +   L   +S       IHPA+ ++GLQ
Sbjct: 181 -------------TRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLGLQ 240

Query: 359 YMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSM 418
           Y  G +SG NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+SF  +CRPLS SM
Sbjct: 241 YSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASM 300

Query: 419 GNAIRFLKNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVL 478
            NAI+FL   I  +  S  E EAK+ L   I R++ EKI+LA + I R A  KI +GDV+
Sbjct: 301 HNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVI 360

Query: 479 LTYGSPCVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISY 538
           L YG   +V  +L  A   G++FRVVVVDSRP LEG+  LR L+  G+  +Y  I A SY
Sbjct: 361 LVYGCSSLVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASY 420

Query: 539 VMREVDRVFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSIC 598
           V+ EV +V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+  
Sbjct: 421 VLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFV 480

Query: 599 FNELGDPDAISKISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTS 646
            NEL DPD +     R +   L +  +   L+LLNL+YD TP + + +++T+ GMIP +S
Sbjct: 481 SNELDDPDDLQ--CKRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIPCSS 517

BLAST of Cp4.1LG04g14290 vs. ExPASy Swiss-Prot
Match: Q3T058 (Translation initiation factor eIF-2B subunit delta OS=Bos taurus OX=9913 GN=EIF2B4 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.1e-87
Identity = 207/480 (43.12%), Postives = 275/480 (57.29%), Query Frame = 0

Query: 170 AGATEKMGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASG 229
           AG   ++G     AK+ +P+      +   +AERRA QEA+RA K + KG+  + G    
Sbjct: 78  AGPDSQLG----TAKEKVPAGR---SKAELRAERRAKQEAERAMKQARKGD--QGGPPPQ 137

Query: 230 ATAGKVGKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKR 289
           A     G+ PS VK                            P   Q DD   + R  K+
Sbjct: 138 ACPSTAGETPSGVK--------------------------RLPEHSQVDDPTLLRRLVKK 197

Query: 290 AVFNQTEAR----NRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDI 349
               Q   R    ++V LF HLPQY +   L   +S       IHPA+ ++GLQY  G +
Sbjct: 198 PERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLGLQYSQGLV 257

Query: 350 SGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRF 409
           SG NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y SF  +CRPLS SM NAI+ 
Sbjct: 258 SGSNARCIALLRALQQVIQDYTTPPSEELSRDLVNKLKPYFSFLTQCRPLSASMYNAIKL 317

Query: 410 LKNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSP 469
           L   I  +  S  E EAK+ L   I R+I EKI+LA + I R A  KI +GDV+L YG  
Sbjct: 318 LNKEITGVSGSKREEEAKSELRAAIDRYIKEKIVLAAQAISRFAYKKISNGDVILVYGCS 377

Query: 470 CVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVD 529
            +V  +L  A   G+QFRVVVVDSRP+LE +  LR L+  G+  +Y  I A SYV+ EV 
Sbjct: 378 SLVSHILQEAWAKGRQFRVVVVDSRPRLEAKHTLRSLVRAGVPASYLLIPAASYVLPEVS 437

Query: 530 RVFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGD 589
           +V LGA A+LANG+V SRVGTA +A+VA A +VPVL CCE YKF ERVQ D+   NEL D
Sbjct: 438 KVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDD 497

Query: 590 PDAISKISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVR 646
           PD +  +  R +   L +  +   L+LLNL+YD TP + + +++T+ GMIP +SVPV++R
Sbjct: 498 PDDL--LCERGEHVALANWQNHSSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLR 518

BLAST of Cp4.1LG04g14290 vs. NCBI nr
Match: XP_023531306.1 (translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1266 bits (3277), Expect = 0.0
Identity = 655/655 (100.00%), Postives = 655/655 (100.00%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVLGNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELEFSEVS 120
           PRHPSDNLSSRSAVLGNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELEFSEVS
Sbjct: 61  PRHPSDNLSSRSAVLGNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELEFSEVS 120

Query: 121 SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEKMGGAS 180
           SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEKMGGAS
Sbjct: 121 SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEKMGGAS 180

Query: 181 TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKVGKQPS 240
           TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKVGKQPS
Sbjct: 181 TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKVGKQPS 240

Query: 241 TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQTEARNR 300
           TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQTEARNR
Sbjct: 241 TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES 420
           QAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES
Sbjct: 361 QAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES 420

Query: 421 EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK 480
           EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK
Sbjct: 421 EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK 480

Query: 481 QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVLANGTV 540
           QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVLANGTV
Sbjct: 481 QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVLANGTV 540

Query: 541 YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY 600
           YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY
Sbjct: 541 YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY 600

Query: 601 LDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655
           LDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV
Sbjct: 601 LDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655

BLAST of Cp4.1LG04g14290 vs. NCBI nr
Match: XP_022933250.1 (translation initiation factor eIF-2B subunit delta-like [Cucurbita moschata])

HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 640/660 (96.97%), Postives = 645/660 (97.73%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASR+VIDPKVRQVGFFTPDAPHSPGG LPA PDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRIVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRHPSDNLSSRS VL     GNWRHPEDLVRVGSHNPSEMLLRSSPMAS S+RIAGEELE
Sbjct: 61  PRHPSDNLSSRSVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
           FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGATEK 180

Query: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKV 240
            GGASTEAKDH PSSSKPLKEKTTKAERRALQEAQR AKVSAKGEANKSGTASGATAGKV
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRVAKVSAKGEANKSGTASGATAGKV 240

Query: 241 GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300
           GKQPSTVKGPATPSVAS DNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT
Sbjct: 241 GKQPSTVKGPATPSVASTDNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300

Query: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360
           EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM
Sbjct: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360

Query: 361 LHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420
           LHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP
Sbjct: 361 LHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420

Query: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480
           SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA
Sbjct: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480

Query: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVL 540
           HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EV+RVFLGAAAVL
Sbjct: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAAVL 540

Query: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR 600
           ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKI GR
Sbjct: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIFGR 600

Query: 601 ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655
           ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV
Sbjct: 601 ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 660

BLAST of Cp4.1LG04g14290 vs. NCBI nr
Match: KAG6587629.1 (Translation initiation factor eIF-2B subunit delta, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1231 bits (3185), Expect = 0.0
Identity = 640/660 (96.97%), Postives = 645/660 (97.73%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASRVVIDPKVRQVGFFTPDAPHSPGG LPA PDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRHPSDNLSSR  VL     GNWRHPEDLVRVGSHNPSEMLLRSSPMAS S+RIAGEELE
Sbjct: 61  PRHPSDNLSSRLVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
           FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNL+TMPANSVRTLGTNKPAG TEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGTTEK 180

Query: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKV 240
            GGASTEAKDH PSSSKPLKEKTTKAERRALQEAQRAAKVS KGEANKSGTASGATAGKV
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRAAKVSGKGEANKSGTASGATAGKV 240

Query: 241 GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300
           GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT
Sbjct: 241 GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300

Query: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360
           EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM
Sbjct: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360

Query: 361 LHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420
           LHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP
Sbjct: 361 LHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420

Query: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480
           SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA
Sbjct: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480

Query: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVL 540
           HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EV+RVFLGAAAVL
Sbjct: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAAVL 540

Query: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR 600
           ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR
Sbjct: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR 600

Query: 601 ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655
           ADISYLDSLTSKE+LQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV
Sbjct: 601 ADISYLDSLTSKENLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 660

BLAST of Cp4.1LG04g14290 vs. NCBI nr
Match: XP_023001776.1 (translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1229 bits (3179), Expect = 0.0
Identity = 639/655 (97.56%), Postives = 644/655 (98.32%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASRVVIDPKVRQVGFFTPDAPHSPGG LP PPDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPTPPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVLGNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELEFSEVS 120
           PRHPSDNLSSRSA  GNWRHPEDLVRVGSHNPSEMLLRSSPMAS STRIAGEELE SEVS
Sbjct: 61  PRHPSDNLSSRSA--GNWRHPEDLVRVGSHNPSEMLLRSSPMASPSTRIAGEELESSEVS 120

Query: 121 SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEKMGGAS 180
           SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNL+TMPANSVRTLGTNKPA ATEKMGGAS
Sbjct: 121 SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAEATEKMGGAS 180

Query: 181 TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKVGKQPS 240
           TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSG+ASGATAGKVGKQPS
Sbjct: 181 TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGSASGATAGKVGKQPS 240

Query: 241 TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQTEARNR 300
           TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDK+RVERAKKRAVFNQTEARNR
Sbjct: 241 TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKNRVERAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ 360
           V LFRHLPQYEQGTQLPDLVSKFFQLDT HPAIYKVGLQYMAGDISGGNARCIAMLHAFQ
Sbjct: 301 VALFRHLPQYEQGTQLPDLVSKFFQLDTFHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES 420
           +AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES
Sbjct: 361 EAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES 420

Query: 421 EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK 480
           EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK
Sbjct: 421 EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK 480

Query: 481 QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVLANGTV 540
           QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV+RVFLGAAAVLANGTV
Sbjct: 481 QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVLANGTV 540

Query: 541 YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY 600
           YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY
Sbjct: 541 YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY 600

Query: 601 LDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655
           LDSLTSKEH QLLNLMYDATPSDYISMIVTDYGMIP TSVPVIVREYRREHLLWV
Sbjct: 601 LDSLTSKEHFQLLNLMYDATPSDYISMIVTDYGMIPATSVPVIVREYRREHLLWV 653

BLAST of Cp4.1LG04g14290 vs. NCBI nr
Match: KAG7021588.1 (Translation initiation factor eIF-2B subunit delta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1222 bits (3161), Expect = 0.0
Identity = 639/669 (95.52%), Postives = 644/669 (96.26%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASRVVIDPKVRQVGFFTPDAPHSPGG LPA PDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRHPSDNLSSR  VL     GNWRHPEDLVRVGSHNPSEMLLRSSPMAS S+RIAGEELE
Sbjct: 61  PRHPSDNLSSRLVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
           FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNL+TMPANSVRTLGTNKPAG TEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGTTEK 180

Query: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKV 240
            GGASTEAKDH PSSSKPLKEKTTKAERRALQEAQRAAKVS KGE NKSGTASGATAGKV
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRAAKVSGKGEVNKSGTASGATAGKV 240

Query: 241 GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300
           GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT
Sbjct: 241 GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300

Query: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360
           EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM
Sbjct: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360

Query: 361 LHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420
           LHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP
Sbjct: 361 LHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420

Query: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480
           SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA
Sbjct: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480

Query: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVL 540
           HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EV+RVFLGAAAVL
Sbjct: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAAVL 540

Query: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR 600
           ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR
Sbjct: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR 600

Query: 601 ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGM---------IPPTSVPVIVRE 655
           ADISYLDSLTSKE+LQLLNLMYDATPSDYISMIVTDYGM         IPPTSVPVIVRE
Sbjct: 601 ADISYLDSLTSKENLQLLNLMYDATPSDYISMIVTDYGMSLPKHGRLQIPPTSVPVIVRE 660

BLAST of Cp4.1LG04g14290 vs. ExPASy TrEMBL
Match: A0A6J1F4E1 (translation initiation factor eIF-2B subunit delta-like OS=Cucurbita moschata OX=3662 GN=LOC111440216 PE=3 SV=1)

HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 640/660 (96.97%), Postives = 645/660 (97.73%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASR+VIDPKVRQVGFFTPDAPHSPGG LPA PDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRIVIDPKVRQVGFFTPDAPHSPGGALPASPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRHPSDNLSSRS VL     GNWRHPEDLVRVGSHNPSEMLLRSSPMAS S+RIAGEELE
Sbjct: 61  PRHPSDNLSSRSVVLPVPASGNWRHPEDLVRVGSHNPSEMLLRSSPMASHSSRIAGEELE 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
           FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNL+TMPANSVRTLGTNKPAGATEK
Sbjct: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAGATEK 180

Query: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKV 240
            GGASTEAKDH PSSSKPLKEKTTKAERRALQEAQR AKVSAKGEANKSGTASGATAGKV
Sbjct: 181 TGGASTEAKDHFPSSSKPLKEKTTKAERRALQEAQRVAKVSAKGEANKSGTASGATAGKV 240

Query: 241 GKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300
           GKQPSTVKGPATPSVAS DNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT
Sbjct: 241 GKQPSTVKGPATPSVASTDNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQT 300

Query: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360
           EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM
Sbjct: 301 EARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAM 360

Query: 361 LHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420
           LHAFQ+AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP
Sbjct: 361 LHAFQEAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPP 420

Query: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480
           SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA
Sbjct: 421 SLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 480

Query: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVL 540
           HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVM EV+RVFLGAAAVL
Sbjct: 481 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMGEVNRVFLGAAAVL 540

Query: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGR 600
           ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKI GR
Sbjct: 541 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIFGR 600

Query: 601 ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655
           ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV
Sbjct: 601 ADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 660

BLAST of Cp4.1LG04g14290 vs. ExPASy TrEMBL
Match: A0A6J1KJK1 (translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495813 PE=3 SV=1)

HSP 1 Score: 1229 bits (3179), Expect = 0.0
Identity = 639/655 (97.56%), Postives = 644/655 (98.32%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARRASRVVIDPKVRQVGFFTPDAPHSPGG LP PPDCTTSLLPDSPTSNSLSPVMIPP
Sbjct: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGALPTPPDCTTSLLPDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVLGNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELEFSEVS 120
           PRHPSDNLSSRSA  GNWRHPEDLVRVGSHNPSEMLLRSSPMAS STRIAGEELE SEVS
Sbjct: 61  PRHPSDNLSSRSA--GNWRHPEDLVRVGSHNPSEMLLRSSPMASPSTRIAGEELESSEVS 120

Query: 121 SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEKMGGAS 180
           SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNL+TMPANSVRTLGTNKPA ATEKMGGAS
Sbjct: 121 SASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLETMPANSVRTLGTNKPAEATEKMGGAS 180

Query: 181 TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGKVGKQPS 240
           TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSG+ASGATAGKVGKQPS
Sbjct: 181 TEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGSASGATAGKVGKQPS 240

Query: 241 TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFNQTEARNR 300
           TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDK+RVERAKKRAVFNQTEARNR
Sbjct: 241 TVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKNRVERAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ 360
           V LFRHLPQYEQGTQLPDLVSKFFQLDT HPAIYKVGLQYMAGDISGGNARCIAMLHAFQ
Sbjct: 301 VALFRHLPQYEQGTQLPDLVSKFFQLDTFHPAIYKVGLQYMAGDISGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES 420
           +AIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES
Sbjct: 361 EAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKLPPSLLES 420

Query: 421 EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK 480
           EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK
Sbjct: 421 EAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYAHDLGK 480

Query: 481 QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVLANGTV 540
           QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV+RVFLGAAAVLANGTV
Sbjct: 481 QFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVNRVFLGAAAVLANGTV 540

Query: 541 YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY 600
           YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY
Sbjct: 541 YSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKISGRADISY 600

Query: 601 LDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLLWV 655
           LDSLTSKEH QLLNLMYDATPSDYISMIVTDYGMIP TSVPVIVREYRREHLLWV
Sbjct: 601 LDSLTSKEHFQLLNLMYDATPSDYISMIVTDYGMIPATSVPVIVREYRREHLLWV 653

BLAST of Cp4.1LG04g14290 vs. ExPASy TrEMBL
Match: A0A6J1BZ97 (translation initiation factor eIF-2B subunit delta-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007085 PE=3 SV=1)

HSP 1 Score: 1135 bits (2937), Expect = 0.0
Identity = 598/662 (90.33%), Postives = 618/662 (93.35%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARR SRVVIDPKVRQVGFFTPDAP SPG T  APPDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRHPSDNL+SR+AVL     GNWRHPED V VGSHNPSE LL SSP+AS S+R+ GEELE
Sbjct: 61  PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
           FSE S+ASVLLRRS SGKLASSCPGVGF+MAAK   NL+TMPANS+RT GTNKPAGATEK
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATEK 180

Query: 181 MGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATA-GK 240
           +GGAS E KDH  SSSKPLK KTTKAERRALQEAQRAAKVSAKGEANKSGTASG TA  K
Sbjct: 181 IGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTAPSK 240

Query: 241 VGKQPSTVKG-PATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300
           VGKQPST+KG PAT SVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRAVFN
Sbjct: 241 VGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRAVFN 300

Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360

Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
           AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
           PP+LLESEAKA L LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS C VEMLL 
Sbjct: 421 PPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLS 480

Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAA 540
           YAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLGAAA
Sbjct: 481 YAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLGAAA 540

Query: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600
           VLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISK+S
Sbjct: 541 VLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKVS 600

Query: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLL 655
           GR D++YLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHL 
Sbjct: 601 GRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHL- 660

BLAST of Cp4.1LG04g14290 vs. ExPASy TrEMBL
Match: A0A6J1BZT6 (translation initiation factor eIF-2B subunit delta-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007085 PE=3 SV=1)

HSP 1 Score: 1130 bits (2923), Expect = 0.0
Identity = 598/665 (89.92%), Postives = 618/665 (92.93%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARR SRVVIDPKVRQVGFFTPDAP SPG T  APPDCTTS L DSPTSNSLSPVMIPP
Sbjct: 1   MDARRGSRVVIDPKVRQVGFFTPDAPPSPGRTTTAPPDCTTSQLSDSPTSNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRHPSDNL+SR+AVL     GNWRHPED V VGSHNPSE LL SSP+AS S+R+ GEELE
Sbjct: 61  PRHPSDNLASRTAVLPVPASGNWRHPEDQVPVGSHNPSETLLGSSPLASPSSRLGGEELE 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGAT-- 180
           FSE S+ASVLLRRS SGKLASSCPGVGF+MAAK   NL+TMPANS+RT GTNKPAGAT  
Sbjct: 121 FSEDSAASVLLRRSSSGKLASSCPGVGFHMAAKSSANLQTMPANSMRTSGTNKPAGATAL 180

Query: 181 -EKMGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATA 240
            EK+GGAS E KDH  SSSKPLK KTTKAERRALQEAQRAAKVSAKGEANKSGTASG TA
Sbjct: 181 AEKIGGASAEVKDHHASSSKPLKTKTTKAERRALQEAQRAAKVSAKGEANKSGTASGTTA 240

Query: 241 -GKVGKQPSTVKG-PATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRA 300
             KVGKQPST+KG PAT SVASADNRGGDR+VDKERKKDAPPPRMQFDDK+RVE+AKKRA
Sbjct: 241 PSKVGKQPSTIKGGPATASVASADNRGGDRMVDKERKKDAPPPRMQFDDKNRVEKAKKRA 300

Query: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360
           VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA
Sbjct: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNA 360

Query: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420
           RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI
Sbjct: 361 RCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRI 420

Query: 421 AKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEM 480
           AKLPP+LLESEAKA L LDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGS C VEM
Sbjct: 421 AKLPPNLLESEAKAALCLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSSCAVEM 480

Query: 481 LLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLG 540
           LL YAH+LGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREV RVFLG
Sbjct: 481 LLSYAHELGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVTRVFLG 540

Query: 541 AAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600
           AAAVLANGTVYSRVGTAGIAMVA AFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS
Sbjct: 541 AAAVLANGTVYSRVGTAGIAMVAHAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAIS 600

Query: 601 KISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 655
           K+SGR D++YLD LT+KEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE
Sbjct: 601 KVSGRTDVNYLDGLTAKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 660

BLAST of Cp4.1LG04g14290 vs. ExPASy TrEMBL
Match: A0A6J1BZS4 (translation initiation factor eIF-2B subunit delta-like OS=Momordica charantia OX=3673 GN=LOC111007076 PE=3 SV=1)

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 526/662 (79.46%), Postives = 569/662 (85.95%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPDSPTSNSLSPVMIPP 60
           MDARR SR+V +PKVRQVGFFT DAP+ P  +L  P DCTTS    SP +NSLSPVMIPP
Sbjct: 1   MDARRGSRIV-NPKVRQVGFFTIDAPNPPSRSLSVPADCTTSPQSHSPLTNSLSPVMIPP 60

Query: 61  PRHPSDNLSSRSAVL-----GNWRHPEDLVRVGSHNPSEMLLRSSPMASSSTRIAGEELE 120
           PRH SDNL+SRS VL      NWR+  D + VGS+NPSE LL +SP+AS S+RI  EEL+
Sbjct: 61  PRHLSDNLASRSTVLPVPAPANWRNSADQIPVGSYNPSETLLGTSPVASPSSRIEDEELD 120

Query: 121 FSEVSSASVLLRRSGSGKLASSCPGVGFYMAAKRGDNLKTMPANSVRTLGTNKPAGATEK 180
            SE S+ SV  RRSGSGK+ASS PGVG ++AAK  +N + +P  + RT   +KP G  EK
Sbjct: 121 LSEDSATSVWYRRSGSGKIASSFPGVGIHVAAKSSENSQAVPLANARTSEPDKPTGVAEK 180

Query: 181 MGGASTEAKDH-LPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGATAGK 240
            GGASTE  +  LP SSKPLK KTTKAERRALQEAQRAAKVSAKGEANKS TA G    K
Sbjct: 181 NGGASTEVPNQQLPPSSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSSTAPG----K 240

Query: 241 VGKQPSTVK-GPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVERAKKRAVFN 300
             KQPS +K  PA PSVAS D RGGDR +DKERKKDAP PRMQFDDK+RVE+AKKRAVFN
Sbjct: 241 AVKQPSAIKDSPAAPSVASTDKRGGDRTLDKERKKDAPHPRMQFDDKNRVEKAKKRAVFN 300

Query: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDISGGNARCI 360
           QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDI GGNARCI
Sbjct: 301 QTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDICGGNARCI 360

Query: 361 AMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420
           AMLHAFQQAIKDYSTPPQKSL RDLTAKIG YVSFFNECRPLSVSMGNAIRFLKNRIAKL
Sbjct: 361 AMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKL 420

Query: 421 PPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLL 480
           P +L ESE KATL  DI+RFINEKI+LADEVIVRHAATKIRDGDVLLTYGS   VEMLLL
Sbjct: 421 PLNLPESEVKATLCSDINRFINEKIILADEVIVRHAATKIRDGDVLLTYGSSSAVEMLLL 480

Query: 481 YAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAA 540
           YAH+LGKQFRVVVVDSRPK+EGQALLRRLI +GLSCTYTHINAISYVM EV RVFLGAAA
Sbjct: 481 YAHELGKQFRVVVVDSRPKMEGQALLRRLITRGLSCTYTHINAISYVMHEVTRVFLGAAA 540

Query: 541 VLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGDPDAISKIS 600
           VL+NGTVYSRVGTAG+AMVA+AFHVPVL CCEAYKFHERVQLDSICFNELGDPDAISK+ 
Sbjct: 541 VLSNGTVYSRVGTAGVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVP 600

Query: 601 GRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLL 655
           GR +I+YLD+LT+KE LQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREH+ 
Sbjct: 601 GREEINYLDNLTAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHV- 656

BLAST of Cp4.1LG04g14290 vs. TAIR 10
Match: AT5G38640.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 711.1 bits (1834), Expect = 8.4e-205
Identity = 406/668 (60.78%), Postives = 484/668 (72.46%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAPHSPGGTLPAPPDCTTSLLPD----SPTSNSLSPV 60
           M++RR  R V+DPKVRQVGFFT   P S        P   TS        SP+ NSLSPV
Sbjct: 1   MESRRNPRAVVDPKVRQVGFFTSSQPDSSTQQQQPDPIAATSASSSSSTISPSGNSLSPV 60

Query: 61  MIPPPR-HPSDNLSSRSAVLGNWRHPEDLVRVGSHNPSEMLL--RSSPMASSSTRIAGEE 120
           MIPPPR + SD    R AV G            + +PS      R      S+T   G  
Sbjct: 61  MIPPPRLNSSDTFPLRVAVAG--------AAASAVSPSSFSYSSRRDFPDGSTTASPGRH 120

Query: 121 LEFSEVSSASVLLRRSGSGKLASSCPGVGF-YMAAKRGDNLKTMPANSVRTLG-TNKPAG 180
              S V S         S  +ASS PG+G   MAA +     ++PA+ + T+     P G
Sbjct: 121 SRGSFVKS---------SVPVASSLPGIGMDSMAAAKS---SSVPASGLTTVSVVTMPPG 180

Query: 181 ATEKMGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVSAKGEANKSGTASGA- 240
            +EK  G   ++        KPLKEKTTKAERRA+QEAQRAAK +AK +  K    S A 
Sbjct: 181 LSEKASGMEVQS-----DQKKPLKEKTTKAERRAIQEAQRAAKAAAKADGGKVAPVSSAS 240

Query: 241 ---TAGKVGK--QPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPPRMQFDDKHRVER 300
              T+ K  K  + ++ K     +  +A+ +GG    +K+RKKDAP PRMQ+DD+ RV +
Sbjct: 241 VASTSVKAAKPAKATSQKNDVAVATGAAEKKGGPS--EKDRKKDAPHPRMQYDDESRVVK 300

Query: 301 AKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAIYKVGLQYMAGDI 360
           AK+RAV  QTEA+NRVELFRHLPQYE GTQLPDL +KFFQLD +HPA+YKVGLQY++GDI
Sbjct: 301 AKRRAVVKQTEAKNRVELFRHLPQYEHGTQLPDLETKFFQLDPMHPAVYKVGLQYLSGDI 360

Query: 361 SGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECRPLSVSMGNAIRF 420
           SGGNARCIAML AFQ+ +KDYSTPP+KSL RD+TAKI  YVSF  ECRPLS+SMGNAIRF
Sbjct: 361 SGGNARCIAMLQAFQEVVKDYSTPPEKSLNRDMTAKISSYVSFLIECRPLSISMGNAIRF 420

Query: 421 LKNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKIRDGDVLLTYGSP 480
           +KNRIAKLP +L ESEAKATL  DI RFINEKI++AD VIV+HA TKIRDGDVLLTYGSP
Sbjct: 421 VKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIVKHAVTKIRDGDVLLTYGSP 480

Query: 481 CVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVD 540
             VEM++L+AH+L K+FRV+VVDSRPKLEGQ LLRRLI +G++CTYTHINAISY+M EV 
Sbjct: 481 TAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRGINCTYTHINAISYIMHEVT 540

Query: 541 RVFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLDSICFNELGD 600
           +VFLGA++VL+NGTVYSRVGTA +AMVA+AF VPVL CCEAYKFHERVQLDSIC NELGD
Sbjct: 541 KVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYKFHERVQLDSICSNELGD 600

Query: 601 PDAISKISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVR 654
           P+AIS++ GR DI+YLD LT+  +LQ LNLMYDATPSDYISMI+TDYGM+PPTSVPVIVR
Sbjct: 601 PNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYISMIITDYGMVPPTSVPVIVR 641

BLAST of Cp4.1LG04g14290 vs. TAIR 10
Match: AT1G48970.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 661.4 bits (1705), Expect = 7.7e-190
Identity = 384/681 (56.39%), Postives = 479/681 (70.34%), Query Frame = 0

Query: 1   MDARRASRVVIDPKVRQVGFFTPDAP------HSPGGTLPAPPDCTTSLLP--DSPTSNS 60
           MD RR +  V  PKVR+VGFFT   P        P  +   P D TTS  P  DSP+ N 
Sbjct: 1   MDTRRGALAV--PKVRRVGFFTSIEPPPESSLQRPNRSQSGPVDATTSSSPLSDSPSGNL 60

Query: 61  LSPVMIPPPRHPSDNLSSR-------------SAVLGNWRHPE-DLVRVGSHNPSEMLLR 120
           +SPV+IPP RH SDNL+SR              A    +  PE  L+ VGS+NP + LL 
Sbjct: 61  ISPVLIPPSRHHSDNLTSRVAAAAAAPVPVPGPAAFRRYLTPERTLLHVGSYNPPDSLLG 120

Query: 121 SSPMASSSTRIAGEELEFSEVSSASVLLRRSGSGKLASSCPGVGF--YMAAKRGDNLKTM 180
           +S  +S+           SE S++    +RS S KL++S P  GF   +A +     +T 
Sbjct: 121 TSSPSSNG--------GLSEDSASLFGFQRSDSTKLSASLPNGGFDLTLAVRAPQESETK 180

Query: 181 PANSVRTLGTNKPAGATEKMGGASTEAKDHLPSSSKPLKEKTTKAERRALQEAQRAAKVS 240
            A +  + G  K A  + K   A+T  K       K LK+KTTKAERRA+QEAQRAAK +
Sbjct: 181 IATTSASQGKKKIAEVSGKSESATT--KPQKEKEPKALKDKTTKAERRAIQEAQRAAKAA 240

Query: 241 AKGEANKSGTASG-ATAGK--VGKQPSTVKGPATPSVASADNRGGDRIVDKERKKDAPPP 300
           AKGE ++    SG A  GK  +  QP   + P T SV+          V+KE++ D P  
Sbjct: 241 AKGEGSRRADESGRANPGKAAIKPQPKKERLPVTSSVSEKTAVA----VEKEKRMDVPQT 300

Query: 301 RMQFDDKHRVERAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDTIHPAI 360
           +MQ+DDK RV++AK+RAV  QTE++N+VELF HLPQYE+  QLP+L S FF LD+IH A+
Sbjct: 301 QMQYDDKSRVDKAKRRAVVEQTESKNKVELFLHLPQYERSNQLPNLSSNFFTLDSIHHAV 360

Query: 361 YKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSFFNECR 420
           YKVGLQ++AGDISG NARCIAML AFQ+AI+DYSTPP K L  DLTAKI  YVSF  ECR
Sbjct: 361 YKVGLQHLAGDISGDNARCIAMLQAFQEAIEDYSTPPMKDLTMDLTAKINGYVSFLIECR 420

Query: 421 PLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRHAATKI 480
           PLS+SMGNAIRFLKN+I KLP +L ESEAK++L  DI RFI+EK ++AD+ IV+HA TKI
Sbjct: 421 PLSMSMGNAIRFLKNQIRKLPVNLSESEAKSSLCSDIGRFIDEKTIIADKAIVQHAVTKI 480

Query: 481 RDGDVLLTYGSPCVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTH 540
           RDG+VLLTYG  CVVEM+LLYAH++GK+FRVV+VDSRP LEGQ LLRRL+ +GL CTYTH
Sbjct: 481 RDGEVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLVTRGLDCTYTH 540

Query: 541 INAISYVMREVDRVFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERV 600
           INAISY+MRE  RVFLGA+++ +NGT+Y+RVGT+ IAMVA+AF VPV+ CCEAYKFHERV
Sbjct: 541 INAISYIMREATRVFLGASSIYSNGTLYARVGTSCIAMVANAFSVPVIVCCEAYKFHERV 600

Query: 601 QLDSICFNELGDPDAISKI-SGRADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDY 654
            LDSIC NELGDPDA++ I S R +  +  ++ + ++LQ LNLMYD+TPS+YISMIV+DY
Sbjct: 601 LLDSICSNELGDPDAVANIPSFRTNAKHSKTMDNNKNLQFLNLMYDSTPSEYISMIVSDY 660

BLAST of Cp4.1LG04g14290 vs. TAIR 10
Match: AT2G44070.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 491.5 bits (1264), Expect = 1.0e-138
Identity = 240/326 (73.62%), Postives = 285/326 (87.42%), Query Frame = 0

Query: 329 IHPAIYKVGLQYMAGDISGGNARCIAMLHAFQQAIKDYSTPPQKSLVRDLTAKIGVYVSF 388
           +H A+YKVGL Y++ DISGGNARCIAML AFQ+ +KDYSTPP+ +L RD+TA+I  YVSF
Sbjct: 1   MHSAVYKVGLHYLSWDISGGNARCIAMLQAFQEVVKDYSTPPENTLNRDMTAQISSYVSF 60

Query: 389 FNECRPLSVSMGNAIRFLKNRIAKLPPSLLESEAKATLSLDISRFINEKIMLADEVIVRH 448
             ECRPLS+SMGNAIRF+KNRIAKLP +L ESEAKA+L  DI RFINEKI+ AD VIV H
Sbjct: 61  LIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINEKIIGADTVIVEH 120

Query: 449 AATKIRDGDVLLTYGSPCVVEMLLLYAHDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLS 508
           A TKIRDGDVLLTYGS  V+EM+L++AH+LGK+FRV VVDSRPKL+G+ LLRRLI +G++
Sbjct: 121 AVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIKRGIN 180

Query: 509 CTYTHINAISYVMREVDRVFLGAAAVLANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYK 568
           CTYTHI AISY+M +V +VFLGA++V +NGTVYSRVGTA +AMVA+AF VPVL CCEAYK
Sbjct: 181 CTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYK 240

Query: 569 FHERVQLD-SICFNELGDPDAISKISGRADISYLDSLTSKEHLQLLNLMYDATPSDYISM 628
           FHE+VQLD SIC NELGDP+AISK+ GR DI+YL+ LT+   L+ LNLMYDATPSDYISM
Sbjct: 241 FHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKFLNLMYDATPSDYISM 300

Query: 629 IVTDYGMIPPTSVPVIVREYRREHLL 654
           I+TDYGM+PPTSVPVIVREY++EHLL
Sbjct: 301 IITDYGMVPPTSVPVIVREYQKEHLL 326

BLAST of Cp4.1LG04g14290 vs. TAIR 10
Match: AT3G07300.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 4.6e-17
Identity = 65/233 (27.90%), Postives = 107/233 (45.92%), Query Frame = 0

Query: 419 ESEAKATLSLDISRFIN---EKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 478
           +S     L  D+   +N   ++I    E I   A   I   +V+LT GS   V   L  A
Sbjct: 169 KSSLTRKLKHDVIEGVNQLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAA 228

Query: 479 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVL 538
            +  + FRV V +  P+ +G  L + L+A+GL  T    +A+  ++  V+ V +GA AV+
Sbjct: 229 KEKKRSFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVM 288

Query: 539 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLD-SICFNELGDPDAISKISG 598
           ANG V   VG    A+ A    VP +    ++K       +  +  NEL  P  +     
Sbjct: 289 ANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 348

Query: 599 RADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREY 648
            +D   LD       LQ++N  +D  P + +S+ +TD G   P+ +  ++ +Y
Sbjct: 349 FSDC--LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADY 399

BLAST of Cp4.1LG04g14290 vs. TAIR 10
Match: AT3G07300.2 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 4.6e-17
Identity = 65/233 (27.90%), Postives = 107/233 (45.92%), Query Frame = 0

Query: 419 ESEAKATLSLDISRFIN---EKIMLADEVIVRHAATKIRDGDVLLTYGSPCVVEMLLLYA 478
           +S     L  D+   +N   ++I    E I   A   I   +V+LT GS   V   L  A
Sbjct: 169 KSSLTRKLKHDVIEGVNQLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAA 228

Query: 479 HDLGKQFRVVVVDSRPKLEGQALLRRLIAKGLSCTYTHINAISYVMREVDRVFLGAAAVL 538
            +  + FRV V +  P+ +G  L + L+A+GL  T    +A+  ++  V+ V +GA AV+
Sbjct: 229 KEKKRSFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVM 288

Query: 539 ANGTVYSRVGTAGIAMVADAFHVPVLFCCEAYKFHERVQLD-SICFNELGDPDAISKISG 598
           ANG V   VG    A+ A    VP +    ++K       +  +  NEL  P  +     
Sbjct: 289 ANGGVIGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGE 348

Query: 599 RADISYLDSLTSKEHLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREY 648
            +D   LD       LQ++N  +D  P + +S+ +TD G   P+ +  ++ +Y
Sbjct: 349 FSDC--LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADY 399