Cp4.1LG04g14280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g14280
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Transcription initiation factor IIE subunit alpha
Location	Cp4.1LG04: 11444540 .. 11450766 (-)
RNA-Seq Expression	Cp4.1LG04g14280
Synteny	Cp4.1LG04g14280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTGAAGAAAAATAAGCCCTAGACTGACGTATAACCCCACCATAACGCGCGTTTTCTTGTTTCGTTCTTCTCCCTCTCCACCGCTGCCATTGTCAGTCAGTCGTCGCCGTCGCCGACGCCTTTCTTCCCTTTCTTCCCTTTCTCCCTATCCACCACTGCCACGCCAACTTACCTTTCTTTCTGGAAAATTTGGATCGAGGCTAATTTTAGTTCAATTTGTTGCCAAATCAGACCTAATTTGGATGCAATTTTTTGGATCGATATATGATCCAAATCCGTTCTGATGATTTGAATCTTTCCAAATCCAGGGGGTTCAAACAGGAGTAATTATTTGATTGAGAAATTGAACTCCAATTTCATTTATCTTCGCCATGAGCATCGAACCTTTTAACAGGTACTTTTCTCTATTTACTCGTGAGCCCTCTCAAAACGATGCAATGGCAGTTTGGATGTCTTCTTTAGCCGTCTCTGAATGCTCGACAATGACGCTGTTTTCTGTTTTGGTTTACTCTCGATCAATTGGGCAGGTTGGTGAAGCTAGCTGCGAGAGCTTTCTATGATGATATTACGACTAAAGGGGATAATCAGCCCAAGACCGGTCGGAGTGATAATAGGGGAATCGCGGTTGTTGTTCTCGATGCTCTCACGAGGTACTGCTCTTTTGCATTTTTATTTACTTGGAAATCTTCAGTCATGGAAGAACACCGCCACTCAATTTGTGCTTACTAAAAAGCAACTGAAAGGAGAGGGGGAGCTAGTTTTATTAGTGGAACGTGTGCTTAAATAATATGATTGGACCTCTGGAAACCTCTTTCTTCATTTTAGAGTATTGTAGTATTAAGCCGATTCTTGTTAGTTATTTTCCTGACTAAGCTGGGCAATATATTTTCACCCCATTATGCTAGTGCTGTAGTGGAACAACTAAATCAATGCATATCATGTTTGTTATATATGTTCCTAAAAACTTATATTGTACGCTTGTTTCTTTAGTAAAGCTAAGTTGAAGAAGGGCAAATTTGATGTAGTTTACTCAATAGTTTAGTTGTACTTCTGTTTCTTTCAAATTGTTAGATTCTTTTGGATGGTCTGAACAAATAGCTAATTTAAAATGTCATTGCCATGTAATTTATTATCATAAAGGATTTGGGATATGGTCCTGCCTAAGGTTTAAAATTTTATGATGATGAGGCCATGCGTGTATGTATCTTCTTCTGTGAATCTGATGAGCTGTTCTACTGCCACATTTATGCTTTGATTATCACATTTGTTGTTGTATCCTGTCCTAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAGTTACGTCGAACCCTAAGGTTTTTAGAAGAGGAAAAACTGATTACTCGAGATCATAGAAGGGAGGTCAGTGCTGACTCTACCTGTGCATCTTGTTCTTGTTTCTATTAGGCTAGTAGCTTGGAATATCTTAGTATTTAATTTTCAGCATTTTAGCTTGACTGTTTATGGCATATAAGGTGGTATTTATGTTGAATGGAGTTTGTGTGTTATTTCGAGAAATTTGTCAAAAGAAACAGAGAAGTGTTTCAAGGAGCAGAAAGGCTGTTGGAGGGTCTTGGGAAGTTACTGTTTTTGTTGCCTCTTGGTGTATTTTTATTAAACTTGTTCGTAATTTCTCTATCTCTCATTTACTTGCCAATAGGAGAGCCTTTTTTCTAATTTGTTGGCTAGAGGGCAGACCGAATGGACCTGCTAAAGACAGATAGTAAAGGGATTTTTAGAGAAAAAAAAAAATGTTTTCTTTGAGAAAATCTCTAAAGTGAATGAATGAAAGGCTCTATGAGCTTTTGTTCAATCTAGACCTTAGATTTCATCAGTGGCCCTTGCACTTGGAGTACTGATAAATATAGTTCATGCTCATCCAGTGATTGACAACTCTACTGCAGTTACTAGTGCCTACATTGCTATACCTTCTTTCTTGGATTAGGCATGCGACAATCCACCTTTTTTCTCTTGTATGTATTACGCCATTTTAGATCTTCACTTGAGGAAGATTGAAAAAGAAGGATACAATTGGCATTAAGTTGAATGATGACCACAATCATCAACTGTGCTCTTTTTCCTCTCTCATCTTATCTCTTATCTTCTTGCATTTCTTCATTTGAGGCTGATTAACATACGGGGTATGTAATCTTGTCTAGTATTTTTCATAATGAAGATTCTATTTGAATTCTGTCAAATATATTGCTTGATAATTCATACTTTTTAATGTGGAGCCGCTGTCTCAATGAGTTACCCTTTTCTAACATGATACCATTATTTATTGGCATGCTCTTCATTTTTTTGTTCTATGTTTTTCCAGACAGCTAAGGGAGCAAAAATATTTAGTGCTGCAGTTGCTGCCACAGCTGATGGTCAACGCTCAACGAAGGAGGGGGATGAAAAGATTAAGATGCACACGCACTCTTATTGCTCGCTAGACTATGCACAGGTTTCTTTTCTATACGTAATTGTTATGGTCGCTTTACTAAATAATAATTGTATCTAAACTCAATGGAAACAGACTTAATTCTGATCAGCTACCTTATTAAAGCTAAACTTGGCTACAGATTTATGATGTGGTTAGATACAGAGTGCACCGCATGAGGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTTCAGGAGTATATATGCCCCAACTGTTCGAGAAGGTTTTTATGATATGTCCTTTCTCTCATAAAAGAATGTTCTTTCTAATATTTAAAACTGGAATCACAGTACACTTGTTATTGACTTCTTACGGTGCAAGCTTAAATAAATTTATGACATTGATTAGGTACACTGCTTTGGATGCCCTGCGATTAATCTCTCTTGAAGATGAATATTTCCACTGTGAAAATTGCAATGGAGAGCTGGTTGCAGAGAGTGACAAACTGGCTGCTGCTCAGGGAGGGGGTGATGGTGATGATAATGCCAGGAAGCGTCGACATGAAAAATTAAAGGACATGCTTCAGAAGATGGAGGTTTGATCACTTGAAATAGAATTTTATTTTCCAATGGGTCGTGCATTTTACTGTATTGGATTTTACATTTAGTTAGTACAATAACCAAAAAGAGATGTATTCTGAATCATGTTCAGTTTCATTTTTTTTACCATATTGATCTTGAAAAGAATTATGTACTGGATATGAATTATGCTAAATGTTGATGAATAAATTGAATTATTGCAGGTACAGCTTAAACCTTTAATGGAACAAGTCAGCAGAGTCAAAGATTTGGCAGTTCCAGAATTTGGAACTCTTTTAGCATGGGAAGCTCATGCAAGTGCAGCTGCACGTGCAAATGGTGATCTGAATGGCAATGATCCTTCAAAATCATCACAAGGTTTAGGATATGGGGGAGCACCAATGCCTTTTGTTGGTGAGACCAAGGTATCCCATTTTTTGCCTTATGCTTGTTATATTGTTGGATCTTGAAAGGCACCATTCTAACAGTCGATGAGTTCTTGAATGAACCTTTTAGGTTGAAGTTGCCTTTTCTGGGGCTGATGGCAAGGGAGCAGATGTTAAATCTGAATCTGAGACGAGTCTGAAAGTCTTACCTCCTTGGATGATCAAGAAAGGAATGAACCTCACGAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTTCTGCTCAGTTTTTTGATGATAAAAAGTCATCGGTCAATGATGATGATAAAACGAATATACAGGCCTGTTCAATTAGATTAGCTATTTTCATTTCTTACTCTTAACTCTTAAGTGCTTTTTTTATCCCAAAGTCAAATAAAAAGAGAATACATGATCTGATGTACTGGTTGACTTATTGTAGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAGCTTGAAGAAGCTGCTAAGAGTGAACAGAATATTTCCAGCACTGAGGTTACAGAAAATAACACAAATTCCAGTCGCCAGGTGGGAATGAAGGCAAAACGTGAAGAAGAGGATGATGATGATATTGAGTGGGAGGAGGCTCCAACAGGGGGTGAGACATTATTTTCTTTTTTTTTCCATGTTTGACATTTTAATTTTTTATGATGTTCATATCTGTTTATTACTCTGCCGTGCAGTATAACTGTATTAGTACCTTGACAGTACCATAAATTAGTAAATGACATAAACATGTCATATGCTTTATCAGTCTCCATATTCGGCATGGATTTAGGGAAAGAAATGTTTTAGTTTGAGAAATAGCTTTTTGGTAATCATCATTTTCTTTTGCGTATGAATTCTATCATCCTTTTGTAGGGGCCTTGCTAATCTTCCCTATATCGTTCCAAGTTTATCAGGGATTTTGTGTATGAATTTTATGTTTCATGTTAAATGTTTTACTCATTATTTATGTTTGGAACTGTGGACTGACTTTCTAGTTTCAAAACTTAATTGATTATTTCTATTAAAAAAGAGCTTGATTGGTTACAAATTGCAATCAAAATCTTATTGTCTTACGCCCTGTCATACCAGACTGGCTTTTCAAATGAATAGTGGCTTCAAAGTTGTGTGTCGACCTTACTTTGGTGTTTTTCGGAGGGCATTTAGTGACTAAATGCTTTATGTTCTCAATTATTGATTTTTCAGTCTTACATTAGATTTGATTTATGTTTCCTAGACCAAGTTTATAAAGATGTTGAAATCAACAGTTTAGTAGAACTTCTTCTTTAATTGGTTATGGCATATTTTGTGTGTTAAGATAAGTTGTGACAGGGAATAACTTCTTTTGGTTCGACTTTATTCAACTATCTATTACTAGATTATTCTTGCCTCAATCACCAAATCCCTTAACTTTCATTCCTTATTACAGGTAATGCAAATGAAAACTACAAGGTTGACTTGAACGTTGAAGCATCAGCTTCGGTAGAGGATGATGAAGAAGAAGATGATGTGGATTGGGAGGAAGGTTGAAGAGACAGAGAAATTTAAAACCCAAATCTGACCAAGGTCATTCCTTTATTCCATCTGCAAGGGCTTAATAAATTGAGGTAGGAGCTCTGCAATTAATCTTCAGCAGCCGTGTTCCTTATTATATTGGAGCCGATTGTAGAGATCCCTAGCCTAAATTGCTTGATTTTCTGCTGATATTTCATCTTGGGCACTCCGATGAATTGAAGTGTTGGAAGAAAAGATAAACGAAAATATATGAATGAAAGAAGAAAGGAATGTCAATCCATCTGTTACCAAATTATAAAGGGATGAACCCGTGTGCAAACTTGCTATTGGAGCAATACGGTATATTTTACAGTAGCTTTTACTTTTTTAGTTGATGGGGTGAAGCTTAGGAATAAAGACAGCGATTGCCAAACATTTTCTTCTTACTCTCCCTGTAGAAACAACTGAACATAATAAATTAGTATCTTTTTAAACATTGCATAACGCATGTGCTTCATTTGTATGTTTATATTTACATTGATTACAATAACAATTAACACTTATTGCCAGATCACAATTTCTTGCAACATTTCTTAGTGGGTCATTAGAGTTTCACCATGTTGTTTATCTGTAGATTGCAGCAGCATGGAACAATTTAGAAGGTAATCCTTTAAGAACGGTTTCTTTTTACATAGTTTAGTCTTAAGAAGACATTGTTTTAGTCATTTCAACAAATTACCTTGCTCTGGATCTTGTATATCTTCTTCATATGTTGCCATCTCTCCCTGCTTTATTCTTATTATCAACCTTCAGGACAGAGGGAGGTTGCTCAACTTCTCAGCCATGTAGTGGACTAGCTGGAAAGAACAGAAGGAATATGAGTAGGAAAGCCTCAAGCCATCAGGATCAAGGCTATCCCTTCTGACTCTGATATGCCAAATGTGAACTCCGAGTGTTATTAGGTGGAAGGTGTCTGTAGTTTAAGCAAAGTCGTCCTAGGTGTCTGTAGTTTTCCCAACCGATGCCGACATTTCTGCATAAGAATTGTCTTCCCCATTTACATCGTGGTGAATGATCGAATGGTCAATATTTTCTAGTGATAAGATATAAAGGCATGATATTGAGAGCTTAGCCGACAAAGTGATTTTGAAAGAAAGCCATTCAAAGGTCATTTTCCCACTTCCGGAAGAAAAGGGGCTGTGCTTTGTTTTTTTAACAAAAAGAAATATTTTGCTTGGGAACTTGGGTTTGCACACTCAATCAT

mRNA sequence

GGTGAAGAAAAATAAGCCCTAGACTGACGTATAACCCCACCATAACGCGCGTTTTCTTGTTTCGTTCTTCTCCCTCTCCACCGCTGCCATTGTCAGTCAGTCGTCGCCGTCGCCGACGCCTTTCTTCCCTTTCTTCCCTTTCTCCCTATCCACCACTGCCACGCCAACTTACCTTTCTTTCTGGAAAATTTGGATCGAGGCTAATTTTAGTTCAATTTGTTGCCAAATCAGACCTAATTTGGATGCAATTTTTTGGATCGATATATGATCCAAATCCGTTCTGATGATTTGAATCTTTCCAAATCCAGGGGGTTCAAACAGGAGTAATTATTTGATTGAGAAATTGAACTCCAATTTCATTTATCTTCGCCATGAGCATCGAACCTTTTAACAGGTTGGTGAAGCTAGCTGCGAGAGCTTTCTATGATGATATTACGACTAAAGGGGATAATCAGCCCAAGACCGGTCGGAGTGATAATAGGGGAATCGCGGTTGTTGTTCTCGATGCTCTCACGAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAGTTACGTCGAACCCTAAGGTTTTTAGAAGAGGAAAAACTGATTACTCGAGATCATAGAAGGGAGACAGCTAAGGGAGCAAAAATATTTAGTGCTGCAGTTGCTGCCACAGCTGATGGTCAACGCTCAACGAAGGAGGGGGATGAAAAGATTAAGATGCACACGCACTCTTATTGCTCGCTAGACTATGCACAGATTTATGATGTGGTTAGATACAGAGTGCACCGCATGAGGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTTCAGGAGTATATATGCCCCAACTGTTCGAGAAGGTACACTGCTTTGGATGCCCTGCGATTAATCTCTCTTGAAGATGAATATTTCCACTGTGAAAATTGCAATGGAGAGCTGGTTGCAGAGAGTGACAAACTGGCTGCTGCTCAGGGAGGGGGTGATGGTGATGATAATGCCAGGAAGCGTCGACATGAAAAATTAAAGGACATGCTTCAGAAGATGGAGGTACAGCTTAAACCTTTAATGGAACAAGTCAGCAGAGTCAAAGATTTGGCAGTTCCAGAATTTGGAACTCTTTTAGCATGGGAAGCTCATGCAAGTGCAGCTGCACGTGCAAATGGTGATCTGAATGGCAATGATCCTTCAAAATCATCACAAGGTTTAGGATATGGGGGAGCACCAATGCCTTTTGTTGGTGAGACCAAGGTTGAAGTTGCCTTTTCTGGGGCTGATGGCAAGGGAGCAGATGTTAAATCTGAATCTGAGACGAGTCTGAAAGTCTTACCTCCTTGGATGATCAAGAAAGGAATGAACCTCACGAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTTCTGCTCAGTTTTTTGATGATAAAAAGTCATCGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAGCTTGAAGAAGCTGCTAAGAGTGAACAGAATATTTCCAGCACTGAGGTTACAGAAAATAACACAAATTCCAGTCGCCAGGTGGGAATGAAGGCAAAACGTGAAGAAGAGGATGATGATGATATTGAGTGGGAGGAGGCTCCAACAGGGGGTAATGCAAATGAAAACTACAAGGTTGACTTGAACGTTGAAGCATCAGCTTCGGTAGAGGATGATGAAGAAGAAGATGATGTGGATTGGGAGGAAGATTGCAGCAGCATGGAACAATTTAGAAGGACAGAGGGAGGTTGCTCAACTTCTCAGCCATGTAGTGGACTAGCTGGAAAGAACAGAAGGAATATGAGTAGGAAAGCCTCAAGCCATCAGGATCAAGGCTATCCCTTCTGACTCTGATATGCCAAATGTGAACTCCGAGTGTTATTAGGTGGAAGGTGTCTGTAGTTTAAGCAAAGTCGTCCTAGGTGTCTGTAGTTTTCCCAACCGATGCCGACATTTCTGCATAAGAATTGTCTTCCCCATTTACATCGTGGTGAATGATCGAATGGTCAATATTTTCTAGTGATAAGATATAAAGGCATGATATTGAGAGCTTAGCCGACAAAGTGATTTTGAAAGAAAGCCATTCAAAGGTCATTTTCCCACTTCCGGAAGAAAAGGGGCTGTGCTTTGTTTTTTTAACAAAAAGAAATATTTTGCTTGGGAACTTGGGTTTGCACACTCAATCAT

Coding sequence (CDS)

ATGAGCATCGAACCTTTTAACAGGTTGGTGAAGCTAGCTGCGAGAGCTTTCTATGATGATATTACGACTAAAGGGGATAATCAGCCCAAGACCGGTCGGAGTGATAATAGGGGAATCGCGGTTGTTGTTCTCGATGCTCTCACGAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAGTTACGTCGAACCCTAAGGTTTTTAGAAGAGGAAAAACTGATTACTCGAGATCATAGAAGGGAGACAGCTAAGGGAGCAAAAATATTTAGTGCTGCAGTTGCTGCCACAGCTGATGGTCAACGCTCAACGAAGGAGGGGGATGAAAAGATTAAGATGCACACGCACTCTTATTGCTCGCTAGACTATGCACAGATTTATGATGTGGTTAGATACAGAGTGCACCGCATGAGGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTTCAGGAGTATATATGCCCCAACTGTTCGAGAAGGTACACTGCTTTGGATGCCCTGCGATTAATCTCTCTTGAAGATGAATATTTCCACTGTGAAAATTGCAATGGAGAGCTGGTTGCAGAGAGTGACAAACTGGCTGCTGCTCAGGGAGGGGGTGATGGTGATGATAATGCCAGGAAGCGTCGACATGAAAAATTAAAGGACATGCTTCAGAAGATGGAGGTACAGCTTAAACCTTTAATGGAACAAGTCAGCAGAGTCAAAGATTTGGCAGTTCCAGAATTTGGAACTCTTTTAGCATGGGAAGCTCATGCAAGTGCAGCTGCACGTGCAAATGGTGATCTGAATGGCAATGATCCTTCAAAATCATCACAAGGTTTAGGATATGGGGGAGCACCAATGCCTTTTGTTGGTGAGACCAAGGTTGAAGTTGCCTTTTCTGGGGCTGATGGCAAGGGAGCAGATGTTAAATCTGAATCTGAGACGAGTCTGAAAGTCTTACCTCCTTGGATGATCAAGAAAGGAATGAACCTCACGAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTTCTGCTCAGTTTTTTGATGATAAAAAGTCATCGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAGCTTGAAGAAGCTGCTAAGAGTGAACAGAATATTTCCAGCACTGAGGTTACAGAAAATAACACAAATTCCAGTCGCCAGGTGGGAATGAAGGCAAAACGTGAAGAAGAGGATGATGATGATATTGAGTGGGAGGAGGCTCCAACAGGGGGTAATGCAAATGAAAACTACAAGGTTGACTTGAACGTTGAAGCATCAGCTTCGGTAGAGGATGATGAAGAAGAAGATGATGTGGATTGGGAGGAAGATTGCAGCAGCATGGAACAATTTAGAAGGACAGAGGGAGGTTGCTCAACTTCTCAGCCATGTAGTGGACTAGCTGGAAAGAACAGAAGGAATATGAGTAGGAAAGCCTCAAGCCATCAGGATCAAGGCTATCCCTTCTGA

Protein sequence

MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKMHTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQVSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVEVAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFDDKKSSDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVGMKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEEDCSSMEQFRRTEGGCSTSQPCSGLAGKNRRNMSRKASSHQDQGYPF

Homology

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BLAST of Cp4.1LG04g14280 vs. ExPASy Swiss-Prot
Match: Q557M8 (General transcription factor IIE subunit 1 OS=Dictyostelium discoideum OX=44689 GN=gtf2e1-1 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.1e-14
Identity = 116/481 (24.12%), Postives = 189/481 (39.29%), Query Frame = 0


Query: 9   LVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQ 68
           LVK+  RAFY D                   AV++   L  ++ +++EDLA  L++  K 
Sbjct: 14  LVKMVIRAFYPD-----------------EYAVIIDGLLREKKRIKDEDLALRLRIQQKY 73

Query: 69  LRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKMHTHSYCSL 128
           +R+ L  L+ + ++     +  AKG              +R +          TH    +
Sbjct: 74  VRKILMDLKGDSMVKSSDVKVEAKG------------PNERGS----------THLLWYI 133

Query: 129 DYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHC 188
           DY  I D+V+Y+++  RKK++     K  VQ Y C  C + YTALD  +L++++     C
Sbjct: 134 DYKHIIDIVKYKLYMFRKKMESVKVQKIDVQTYKCQTCHKVYTALDIPKLLNMDTGALAC 193

Query: 189 ENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQVSRVKDLA 248
           E C+GEL  E +  +  Q      D                +  QL+ ++EQ+ + +   
Sbjct: 194 EICDGELEEELNNESLTQTAKHQSD----------------LFSQLRKIIEQLKKTEGHN 253

Query: 249 VPEFGTLLA---WEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVEVAF-- 308
           +P F   LA    +   S     N  L G  P  S+  +  G A    +  T   + F  
Sbjct: 254 IPLFARDLADLSADQGPSYTINTNSSL-GMGPKPSAFPVAQGAATSHHIDPTNENIEFHV 313

Query: 309 -----SGADGKGADVKSES-ETSLKVLPPWMIK----KGMNL---------------TKE 368
                 G +   A VK E+ +T L  LPPW++     K  N+               T E
Sbjct: 314 DILDTDGIEINKAVVKKENKKTGLASLPPWLLPSNSFKNRNVKSNSILNNNQQTQTSTNE 373

Query: 369 QRGEVKEESKMDAGSASAQFFDDKKSSDEYVKAYYAAILKKQQELEEAAKSEQNISSTEV 428
           Q   VKE+ K+D       F+ +      Y+K +Y       QE E A        S + 
Sbjct: 374 QPTAVKEQIKID-----QDFYIN------YIKTHY-------QEWESAP------DSGDA 403

Query: 429 TENNTNSSRQVGMKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDD 460
             N +NS                 IE  +   G + N+  K+D +   S+  + D++  D
Sbjct: 434 DGNGSNSG-----------SGGSTIEGNDGGNGEHQNKKMKLDDSQTVSSMSQSDDDGKD 403

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy Swiss-Prot
Match: A6QLI8 (General transcription factor IIE subunit 1 OS=Bos taurus OX=9913 GN=GTF2E1 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 7.7e-09
Identity = 61/235 (25.96%), Postives = 101/235 (42.98%), Query Frame = 0


Query: 42  VVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAV 101
           + LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA          
Sbjct: 32  LALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA---------- 91

Query: 102 AATADGQRSTKEGDEKIKMHTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEY 161
              ADG           K   H+Y  ++Y  + +VV+Y++  MR++++ +  D      +
Sbjct: 92  ---ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASF 151

Query: 162 ICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHE 221
            CP CS  +T L+A +L       F C  C  E+              + D++A  ++  
Sbjct: 152 KCPVCSSTFTDLEANQLFDPMTGTFRCTFCQTEV--------------EEDESAMPKK-- 211

Query: 222 KLKDMLQKMEVQLKPL------MEQVSRVKDLAVPEFGTLLAWEAHASAAARANG 271
             + +L +   Q++P+       E V+   ++  PE   + A +     AA A G
Sbjct: 212 DARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDRAATAAG 226

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy Swiss-Prot
Match: Q9D0D5 (General transcription factor IIE subunit 1 OS=Mus musculus OX=10090 GN=Gtf2e1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.9e-08
Identity = 60/236 (25.42%), Postives = 102/236 (43.22%), Query Frame = 0


Query: 41  VVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAA 100
           V+ LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA         
Sbjct: 31  VLALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA--------- 90

Query: 101 VAATADGQRSTKEGDEKIKMHTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQE 160
               ADG           K   H+Y  ++Y  + +VV+Y++  MR++++ +  +      
Sbjct: 91  ----ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERNSTNRAS 150

Query: 161 YICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRH 220
           + CP C   +T L+A +L       F C  C+ E+              + D++A  ++ 
Sbjct: 151 FKCPVCCSTFTDLEANQLFDPMTGTFRCTFCHTEV--------------EEDESAMPKK- 210

Query: 221 EKLKDMLQKMEVQLKPL------MEQVSRVKDLAVPEFGTLLAWEAHASAAARANG 271
              + +L +   Q++P+       E V+   ++  PE   + A +     AA A G
Sbjct: 211 -DARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDRAATAAG 226

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy Swiss-Prot
Match: P36100 (Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TFA1 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.6e-06
Identity = 101/502 (20.12%), Postives = 200/502 (39.84%), Query Frame = 0


Query: 38  GIAVVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIF 97
           G  V+VLDA+     + E+DL + L ++  +L   +  L  ++LI+   +RE    +K  
Sbjct: 23  GSFVLVLDAILFHSVLAEDDLKQLLSINKTELGPLIARLRSDRLISIHKQREYPPNSK-- 82

Query: 98  SAAVAATADGQRSTKEGDEKIKMHTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNT 157
                             E++      Y  + Y    D ++++VH++ ++LKD+L+  + 
Sbjct: 83  ----------------SVERV------YYYVKYPHAIDAIKWKVHQVVQRLKDDLDKNSE 142

Query: 158 VQEYICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARK 217
              Y+CP C  +YT L+A++L++ +   F C  C+  LV               +D++ K
Sbjct: 143 PNGYMCPICLTKYTQLEAVQLLNFDRTEFLCSLCDEPLV---------------EDDSGK 202

Query: 218 RRHEKLKDMLQKMEVQLKPLMEQVSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDP 277
           +  EK +D L ++  Q++P+++ + ++ D  + E    +A         +++     N  
Sbjct: 203 KNKEK-QDKLNRLMDQIQPIIDSLKKIDDSRIEENTFEIALARLIPPQNQSHAAYTYNPK 262

Query: 278 SKSSQGLGYGGAPMPFVGETKVEVAFSGADGKGADV---------------------KSE 337
             S+       AP+P +  T +    S   G  +                       ++E
Sbjct: 263 KGSTMFRPGDSAPLPNLMGTALGNDSSRRAGANSQATLHINITTASDEVAQRELQERQAE 322

Query: 338 SETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMD---AGSASAQFFDD------------ 397
            +     +P W   K   + K   G +  E + D     SA      D            
Sbjct: 323 EKRKQNAVPEW--HKQSTIGKTALGRLDNEEEFDPVVTASAMDSINPDNEPAQETSYQNN 382

Query: 398 ---------KKSSDEYVKAYYAAILKKQ----------QELEEAAKSEQNISSTEVTENN 457
                    ++ +++ +  YYAA+ KKQ          +E EE  + E+     +V ++N
Sbjct: 383 RTLTEQEMEERENEKTLNDYYAALAKKQAKLNKEEEEEEEEEEDEEEEEEEEMEDVMDDN 442

Query: 458 TNSSRQVGMK------------AKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASAS- 465
             ++R+  ++            AK E    +D++ E      + N+  +  +N  A+AS 
Sbjct: 443 DETARENALEDEFEDVTDTAGTAKTESNTSNDVKQE------SINDKTEDAVNATATASG 476

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy Swiss-Prot
Match: Q9P3W1 (Transcription initiation factor IIE subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tfa1 PE=1 SV=3)

HSP 1 Score: 54.3 bits (129), Expect = 4.7e-06
Identity = 111/495 (22.42%), Postives = 194/495 (39.19%), Query Frame = 0


Query: 4   EPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLK 63
           E   RL+K+  RAFY+                     ++ +DA+ R   + +E  A  + 
Sbjct: 6   EIVQRLIKMIMRAFYET------------------RHIIFMDAILRHSALTDEQTALLMG 65

Query: 64  LHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKMHTH 123
           +  K+ R     L E++L+    R E                      KEG ++ + HT 
Sbjct: 66  IPIKECRFIAGKLREDRLLAIQSRTE---------------------MKEGQQR-QYHT- 125

Query: 124 SYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLED 183
           +Y  +D+    D +++R+H++ K ++D + +    + Y+CP C++++++LD L L++ E 
Sbjct: 126 TYFYIDFCSTIDSIKWRMHQLVKTVEDRMRNDFDSKGYVCPFCNKKFSSLDVLSLVTNEG 185

Query: 184 EYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQVSR 243
             F C  C  EL              D +++A     +K    L K+  Q+  +++ + R
Sbjct: 186 T-FACNVCGTEL-------------KDDEESAEMMSSQK---RLGKLMGQVNGIIDALKR 245

Query: 244 VKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVEVAF 303
           V ++ VP+     A E HA   +    +L+  + SKS+  +              + V F
Sbjct: 246 VDEIVVPQNNFQSALE-HAVPVSLDTQNLSQQNLSKSNSDVRLS------TSSPSITVDF 305

Query: 304 SGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAG-----SASAQF 363
           S    K  D K E     +V     I    + T    G +      DA      + +   
Sbjct: 306 SA--DKETDEKRERNCDKQVKAAQNILPEWHATSTISGSITRAGAKDAALHSFRTETVNE 365

Query: 364 FDDKK----SSDEYVKAYYAAILKKQQELEEAAKSE-------------------QNIS- 423
             D K    S    + AYYA +  KQ+E  E   SE                   QN S 
Sbjct: 366 VQDTKTDITSEKSALDAYYATLRAKQKEESEFMDSENVDDEEDDDFLDVTTATSLQNKST 425

Query: 424 ---STEVTENNTNSSRQVGMKAKREEEDDDDIEWEEAPTG--GNANENYKVDLNVEASAS 464
              S +    N NS   +  K  +  E+++ +    +  G      E  +   NV  +  
Sbjct: 426 DYGSVKRKTENLNSDSDIQNKRTKSIEENNSLPPIVSTNGITDGDTEMQESKKNVIINGF 433

[Close]

BLAST of Cp4.1LG04g14280 vs. NCBI nr
Match: XP_023531309.1 (general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 463/473 (97.89%), Postives = 463/473 (97.89%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300
           VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE
Sbjct: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300

Query: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360
           VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD
Sbjct: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360

Query: 361 DKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420
           DKKSS          DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG
Sbjct: 361 DKKSSVNDDDKTNIQDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420

Query: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE
Sbjct: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 473

[Close]

BLAST of Cp4.1LG04g14280 vs. NCBI nr
Match: XP_023006507.1 (general transcription factor IIE subunit 1-like [Cucurbita maxima])

HSP 1 Score: 881 bits (2276), Expect = 0.0
Identity = 462/473 (97.67%), Postives = 462/473 (97.67%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQR TKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRPTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300
           VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE
Sbjct: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300

Query: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360
           VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD
Sbjct: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360

Query: 361 DKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420
           DKKSS          DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG
Sbjct: 361 DKKSSVNDDDKTNIQDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420

Query: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE
Sbjct: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 473

[Close]

BLAST of Cp4.1LG04g14280 vs. NCBI nr
Match: XP_022933237.1 (general transcription factor IIE subunit 1-like [Cucurbita moschata] >KAG6587625.1 General transcription factor IIE subunit 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021586.1 General transcription factor IIE subunit 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 460/473 (97.25%), Postives = 461/473 (97.46%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQR TKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRPTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300
           VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE
Sbjct: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300

Query: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360
           VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD
Sbjct: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360

Query: 361 DKKS----------SDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420
           DKKS           DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNS+RQVG
Sbjct: 361 DKKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSNRQVG 420

Query: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE
Sbjct: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 473

[Close]

BLAST of Cp4.1LG04g14280 vs. NCBI nr
Match: XP_038880289.1 (general transcription factor IIE subunit 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 811 bits (2094), Expect = 2.99e-293
Identity = 434/477 (90.99%), Postives = 446/477 (93.50%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ   KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYR+HRM+KKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
            EDEYFHCENCNGELVAESDKLAAAQGG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAAQGG-DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAAR-ANGDLNGNDPSKSSQGLGYGGAPMPFVGETKV 300
           +SRVKDL VPEFGTLLAWEA ASAAAR ANGDLNGNDPSKS+QGLGYGG PMPFVGETKV
Sbjct: 241 LSRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSAQGLGYGGTPMPFVGETKV 300

Query: 301 EVAFSGADGKGADVKSESE-TSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQF 360
           EVAFSGA+GKG DVKSESE T LKVLPPWMIKKGMNLTKEQRGEVK+ESKMDAGSASAQF
Sbjct: 301 EVAFSGAEGKGVDVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKDESKMDAGSASAQF 360

Query: 361 FDDKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTEN--NTNSS 420
            DDKKSS          DEYVKAYYAAILKKQQELEEAAK ++ + STEVTEN  N NSS
Sbjct: 361 SDDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQEELPSTEVTENVANRNSS 420

Query: 421 RQVGMKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           RQVGMKAKREE+++DDIEWEEAP GGNANENYKVDLNVEASASVED EEEDDVDWEE
Sbjct: 421 RQVGMKAKREEDEEDDIEWEEAPIGGNANENYKVDLNVEASASVED-EEEDDVDWEE 475

[Close]

BLAST of Cp4.1LG04g14280 vs. NCBI nr
Match: XP_038880290.1 (general transcription factor IIE subunit 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 804 bits (2076), Expect = 1.59e-290
Identity = 433/477 (90.78%), Postives = 445/477 (93.29%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ   KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYR+HRM+KKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
            EDEYFHCENCNGELVAESDKLAAAQGG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAAQGG-DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAAR-ANGDLNGNDPSKSSQGLGYGGAPMPFVGETKV 300
           +SRVKDL VPEFGTLLAWEA ASAAAR ANGDLNGNDPSKS+QGLGYGG PMPFVGETKV
Sbjct: 241 LSRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSAQGLGYGGTPMPFVGETKV 300

Query: 301 EVAFSGADGKGADVKSESE-TSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQF 360
           EVAFSGA+GKG DVKSESE T LKVLPPWMIKKGMNLTKEQRGEVK+ESKMDAGSASAQF
Sbjct: 301 EVAFSGAEGKGVDVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKDESKMDAGSASAQF 360

Query: 361 FDDKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTEN--NTNSS 420
            DDKKSS          DEYVKAYYAAILKKQQELEEAAK ++ + STEVTEN  N NSS
Sbjct: 361 SDDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQEELPSTEVTENVANRNSS 420

Query: 421 RQVGMKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           RQVGMKAKREE+++DDIEWEEAP G NANENYKVDLNVEASASVED EEEDDVDWEE
Sbjct: 421 RQVGMKAKREEDEEDDIEWEEAPIG-NANENYKVDLNVEASASVED-EEEDDVDWEE 474

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy TrEMBL
Match: A0A6J1KW18 (Transcription initiation factor IIE subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111499214 PE=3 SV=1)

HSP 1 Score: 881 bits (2276), Expect = 0.0
Identity = 462/473 (97.67%), Postives = 462/473 (97.67%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQR TKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRPTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300
           VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE
Sbjct: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300

Query: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360
           VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD
Sbjct: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360

Query: 361 DKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420
           DKKSS          DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG
Sbjct: 361 DKKSSVNDDDKTNIQDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420

Query: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE
Sbjct: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 473

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy TrEMBL
Match: A0A6J1EZ78 (Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111440157 PE=3 SV=1)

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 460/473 (97.25%), Postives = 461/473 (97.46%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQR TKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRPTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300
           VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE
Sbjct: 241 VSRVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVE 300

Query: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360
           VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD
Sbjct: 301 VAFSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFD 360

Query: 361 DKKS----------SDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSSRQVG 420
           DKKS           DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNS+RQVG
Sbjct: 361 DKKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTENNTNSNRQVG 420

Query: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE
Sbjct: 421 MKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 473

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy TrEMBL
Match: A0A6J1BZ86 (Transcription initiation factor IIE subunit alpha OS=Momordica charantia OX=3673 GN=LOC111007077 PE=3 SV=1)

HSP 1 Score: 797 bits (2058), Expect = 4.55e-288
Identity = 435/479 (90.81%), Postives = 447/479 (93.32%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ+  KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYR+HRM+KKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGG-DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAAR-ANGDLNGNDPSKSSQGLGYGGAPMPFVGETKV 300
           +SRVKDL VPEFGTLLAWEA ASAAAR ANGDLNGNDPSKSSQGLGYGG PMPFVGETKV
Sbjct: 241 LSRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKV 300

Query: 301 EVAFSGADGKGADVKSESE-TSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQF 360
           EVAFSGA+GKG DVKSE+E T LKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA AQF
Sbjct: 301 EVAFSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQF 360

Query: 361 FDDKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTEN--NTNSS 420
            DDKKSS          DEYVKAYYAAILKKQQELEE+AK  + +SSTEVTE+  N NSS
Sbjct: 361 SDDKKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSS 420

Query: 421 RQVGMKAKRE-EEDDDDIEWEEAPTGGNANENYKV-DLNVEASASVEDDEEEDDVDWEE 463
           RQVGMKAKRE +E+DDDIEWEEAP GGNANENYKV DLNVEASASVED EEEDDVDWEE
Sbjct: 421 RQVGMKAKREGDEEDDDIEWEEAPIGGNANENYKVGDLNVEASASVED-EEEDDVDWEE 476

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy TrEMBL
Match: A0A6J1E5J2 (Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111430131 PE=3 SV=1)

HSP 1 Score: 796 bits (2055), Expect = 1.35e-287
Identity = 431/479 (89.98%), Postives = 448/479 (93.53%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQ   KEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQ+YDVVRYR+HRM+KKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
           LEDEYFHCENCNGELVAESDKLAAAQGG DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ
Sbjct: 181 LEDEYFHCENCNGELVAESDKLAAAQGG-DGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAAR-ANGDLNGNDPSKSSQGLGYGGAPMPFVGETKV 300
           +SRVKDL VPEFGTLLAWEA ASAAAR ANGDLNGNDPSKSSQGLGYGG PMPFVGETKV
Sbjct: 241 LSRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKV 300

Query: 301 EVAFSGADGKGADVKSESE-TSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQF 360
           EVAFSGA+GKG +VKSESE T+LKVLPPWMIK+GMNLTKEQRGEVK +SKMDAGSASAQF
Sbjct: 301 EVAFSGAEGKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGDSKMDAGSASAQF 360

Query: 361 FDDKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTEN--NTNSS 420
            DDKKSS          DEYVKAYYAAILKKQQELEEAAK+++ +SSTEVTEN   TNSS
Sbjct: 361 SDDKKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKAQKELSSTEVTENLSYTNSS 420

Query: 421 RQVGMKAKREE-EDDDDIEWEEAPTGGNANENYKV-DLNVEASASVEDDEEEDDVDWEE 463
           RQVGMKAKREE E++DDIEWEEAP  GNANENYKV DLNVEASASVED EEEDDVDWEE
Sbjct: 421 RQVGMKAKREEDEEEDDIEWEEAPIEGNANENYKVGDLNVEASASVED-EEEDDVDWEE 477

[Close]

BLAST of Cp4.1LG04g14280 vs. ExPASy TrEMBL
Match: A0A5D3DYI4 (Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00230 PE=3 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 2.84e-286
Identity = 429/478 (89.75%), Postives = 441/478 (92.26%), Query Frame = 0


Query: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60
           MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK
Sbjct: 1   MSIEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAK 60

Query: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKM 120
           NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ  TKEGDEKIKM
Sbjct: 61  NLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHQTKEGDEKIKM 120

Query: 121 HTHSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180
           HTHSYCSLDYAQIYDVVRYR+HRM+KKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS
Sbjct: 121 HTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS 180

Query: 181 LEDEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQ 240
            EDEYFHCENCNGELVAESDKLAA QGG DGDDNARKRRHEKLKDMLQKME QLKPL+EQ
Sbjct: 181 FEDEYFHCENCNGELVAESDKLAAVQGG-DGDDNARKRRHEKLKDMLQKMEAQLKPLVEQ 240

Query: 241 VSRVKDLAVPEFGTLLAWEAHASAAARA-NGDLNGNDPSKSSQGLGYGGAPMPFVGETKV 300
           ++RVKDL VPEFGTLLAWEA ASAAAR  NGDLNGNDPSKSSQG  YGG PMPFVGETKV
Sbjct: 241 LTRVKDLPVPEFGTLLAWEARASAAARGVNGDLNGNDPSKSSQG--YGGTPMPFVGETKV 300

Query: 301 EVAFSGADGKGADVKSESE-TSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQF 360
           EVAFSGADGKG DVKSES+ T LKVLPPWMIK+GMNLTKEQRGEVK+ESK DAGSASAQF
Sbjct: 301 EVAFSGADGKGVDVKSESKNTGLKVLPPWMIKQGMNLTKEQRGEVKDESKTDAGSASAQF 360

Query: 361 FDDKKS----------SDEYVKAYYAAILKKQQELEEAAKSEQNISSTEVTEN--NTNSS 420
            DDKKS           DEYVKAYYAAILKKQQELEEAAK +Q +SSTEVTE+  NTNSS
Sbjct: 361 SDDKKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQQELSSTEVTESVANTNSS 420

Query: 421 RQVGMKAKREEEDDDD-IEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 463
           RQVGMKAKREE+DDDD IEWEEAP GGNANENYKVDLNVEASA VED EEEDDVDWEE
Sbjct: 421 RQVGMKAKREEDDDDDDIEWEEAPIGGNANENYKVDLNVEASALVED-EEEDDVDWEE 474

[Close]

BLAST of Cp4.1LG04g14280 vs. TAIR 10
Match: AT1G03280.1 (Transcription factor TFIIE, alpha subunit )

HSP 1 Score: 548.1 bits (1411), Expect = 7.3e-156
Identity = 312/482 (64.73%), Postives = 378/482 (78.42%), Query Frame = 0


Query: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++PF +LV+L ARAFYDD TTK DNQ K+ RSDNRGIA VVLDAL RRQWVREEDLAK+L
Sbjct: 13  LQPFVKLVRLVARAFYDDYTTKSDNQQKSARSDNRGIAAVVLDALARRQWVREEDLAKDL 72

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKMHT 122
           +LH+KQLR+ +R  EEEKLI RDHR+ETAKGAK++SAAVAAT DG+      ++K+K+HT
Sbjct: 73  QLHAKQLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAAVAATTDGR-----AEDKVKLHT 132

Query: 123 HSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYC LDYAQI DVVR+R+HRM+K+LKDELEDKNTVQEY CPNC R+Y ALDALRLIS+ 
Sbjct: 133 HSYCCLDYAQICDVVRFRLHRMKKRLKDELEDKNTVQEYGCPNCQRKYNALDALRLISMV 192

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQVS 242
           D+ FHCENCNGELV E +KL + +   DGDDNAR+RR E LK+MLQK+EVQ+KPLM+Q++
Sbjct: 193 DDSFHCENCNGELVVECNKLTSEE-VVDGDDNARRRRRENLKNMLQKLEVQMKPLMDQLN 252

Query: 243 RVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVEVA 302
           RVKDL +PEFG+ LAWEA A+ AAR NGDLN NDP + SQG GYG  PMPF+GETKVEV 
Sbjct: 253 RVKDLPIPEFGSFLAWEARAAMAARENGDLNPNDPLR-SQG-GYGSTPMPFLGETKVEVN 312

Query: 303 FSGADGKGADVKSE-SETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGS-ASAQFFD 362
               DG   DVKS+  ++SLKVLPPWMIK+GMNLT+EQRGE+++E+K+D G+ A+A+  D
Sbjct: 313 L--GDG-NEDVKSKGGDSSLKVLPPWMIKEGMNLTEEQRGEMRQEAKVDGGAGAAAKLSD 372

Query: 363 DKKSS----------DEYVKAYYAAILKKQQELEEAAKSEQNISSTEVT----ENNTNSS 422
           DKKS+          DEY+KAYYA ++K+Q   E AA+  Q  S+ E T         S 
Sbjct: 373 DKKSAIGNGDEKDLKDEYLKAYYAELMKQQ---ELAARRNQQESAGEPTSGIQSGTVYSG 432

Query: 423 RQVGMKAKREEEDDDD---IEWEE-APTGGNANENYKVDLNVEASAS-VEDDEEEDDVDW 464
           RQV MKAKREE++D+D   +EWEE AP    AN NYKVDLNVEA AS  E++EEEDDVDW
Sbjct: 433 RQVSMKAKREEDEDEDEEEVEWEEKAPV--TANGNYKVDLNVEAEASGGEEEEEEDDVDW 478

[Close]

BLAST of Cp4.1LG04g14280 vs. TAIR 10
Match: AT4G20340.1 (Transcription factor TFIIE, alpha subunit )

HSP 1 Score: 459.1 bits (1180), Expect = 4.5e-129
Identity = 262/480 (54.58%), Postives = 343/480 (71.46%), Query Frame = 0


Query: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           +EPF +LV+L  R FYD+ T + DNQ K+ + + +G AV+VLDALTRRQWVREEDLAK +
Sbjct: 14  LEPFVKLVRLLVRIFYDNYTPESDNQQKSVK-NVKGSAVIVLDALTRRQWVREEDLAKEV 73

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKMHT 122
           K ++K+LR+ +R  EE+K + R HR+ETAK AK++S AV  T DG+      ++ +K HT
Sbjct: 74  KRNAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAVGGTTDGR-----AEDNVKFHT 133

Query: 123 HSYCSLDYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYC LDYAQIYD+VRY++HR++KK KDELED+NTVQEY CPNC R+Y ALDALRLIS+E
Sbjct: 134 HSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCPNCKRKYNALDALRLISME 193

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQVS 242
           D+ FHCENCNGELV E +KL + +   D  DNAR+R+ EK+K  LQ +E +LKPLME ++
Sbjct: 194 DDSFHCENCNGELVMECNKLISEE-VVDRGDNARRRQREKVKVWLQDLEGELKPLMELIN 253

Query: 243 RVKDLAVPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVEVA 302
           RVKDL  P F    AWEA A+ AAR NGD N +DPS+S    GYG  PMPF+GETKVEV 
Sbjct: 254 RVKDLPFPAFEPFPAWEARAAKAARENGDFNPDDPSRSLG--GYGSTPMPFLGETKVEVN 313

Query: 303 FSGADGKGADVKSESETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFDDK 362
               +G      +  ++SLK+LPPWMIK+GM LT+EQRGE+++E+ +D    +A+  DDK
Sbjct: 314 L--GEGNEDVTSTGGDSSLKMLPPWMIKQGMKLTEEQRGEMRQEANVD--GEAAKLSDDK 373

Query: 363 KS-----------SDEYVKAYYAAILKKQQ------ELEEAAKSEQNISSTEVTENNTNS 422
           KS            DEY+KAYYAAI+++Q+      E E A +S    ++T++    T S
Sbjct: 374 KSVMENGDDNKDLKDEYLKAYYAAIMEQQKLAAKLNEQESAGES----TTTDIESATTYS 433

Query: 423 SRQVGMKAKREEEDDDDIEWEEAPTGGNANENYKVDLNVEA--SASVEDDEEEDDVDWEE 464
            RQVGMK+KREEE ++D+EWEE  +   AN NYKVDLNVEA  +   ED +E+DD+DWEE
Sbjct: 434 DRQVGMKSKREEE-EEDVEWEEGASVA-ANGNYKVDLNVEAEEAEEKEDGDEDDDIDWEE 474

[Close]

BLAST of Cp4.1LG04g14280 vs. TAIR 10
Match: AT4G20810.1 (transcription initiation factor IIE (TFIIE) alpha subunit family protein / general transcription factor TFIIE family protein )

HSP 1 Score: 388.3 bits (996), Expect = 9.7e-108
Identity = 232/461 (50.33%), Postives = 314/461 (68.11%), Query Frame = 0


Query: 9   LVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQ 68
           +VKL A+ FYD+ T K +NQ K+ ++ + GIAV+VLDALTRRQWVREEDLAK LKL++KQ
Sbjct: 1   MVKLVAKTFYDNYTPKNNNQKKSAKNGSGGIAVLVLDALTRRQWVREEDLAKELKLNTKQ 60

Query: 69  LRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQRSTKEGDEKIKMHTHSYCSL 128
           LR  LR+ EE++ I R HR+E +          +AT +G+     G++K+K+H +SYC L
Sbjct: 61  LRTILRYFEEQQFIMRVHRKEKS----------SATTNGR-----GEDKVKVHMYSYCCL 120

Query: 129 DYAQIYDVVRYRVHRMRKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHC 188
           DY+QIYDV+RY++HRM+K+ KD LEDK+ VQEY CPNC R+               +FHC
Sbjct: 121 DYSQIYDVIRYKLHRMKKEFKDVLEDKDNVQEYGCPNCKRKI--------------FFHC 180

Query: 189 ENCNGELVAESDKLAAAQGGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQVSRVKDLA 248
           ENCNGELV E +KL + +   DG DN R RR + LKD+LQ MEV+LKPLM+ ++R+KDL 
Sbjct: 181 ENCNGELVMECNKLTSEEVVVDGSDNPRSRR-DHLKDLLQNMEVRLKPLMDHINRIKDLP 240

Query: 249 VPEFGTLLAWEAHASAAARANGDLNGNDPSKSSQGLGYGGAPMPFVGETKVEVAFSGADG 308
           VP F +  AWE   + AAR NGDLN +D  +  QG GYG  PMPF+GET++EV       
Sbjct: 241 VPSFESFPAWETRVAKAARENGDLNPDDTLR-PQG-GYGSTPMPFLGETEIEV---NLGE 300

Query: 309 KGADVKSE---SETSLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSASAQFFDDKKS- 368
           +  DVKS+     +  K+ P W+IKKGMNL+ EQRGE++ E+K D G +S +  DD ++ 
Sbjct: 301 ENEDVKSDEVGDSSRRKLTPSWLIKKGMNLSDEQRGEIRHEAKADDGGSSMENGDDDRNL 360

Query: 369 SDEYVKAYYAAILKKQQELEEAAKSEQNIS--STEVTENNTNSSRQVGMKAKREEEDDDD 428
            DEY+KAYYAAIL ++QEL E    +++    +T++    ++S RQVGMK+KREEE++  
Sbjct: 361 KDEYLKAYYAAIL-EEQELAEKLNQQESAGKVTTDIELATSSSDRQVGMKSKREEEEE-- 415

Query: 429 IEWEEAPTGGNANENYKVDLNVEASASVEDDEEEDDVDWEE 464
              EEA     AN NYKVDLNVEA    E +++E+DVDW+E
Sbjct: 421 ---EEASVA--ANGNYKVDLNVEAE---EAEQDENDVDWQE 415

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g14280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q557M8	6.1e-14	24.12	General transcription factor IIE subunit 1 OS=Dictyostelium discoideum OX=44689 ...	[more]
A6QLI8	7.7e-09	25.96	General transcription factor IIE subunit 1 OS=Bos taurus OX=9913 GN=GTF2E1 PE=2 ...	[more]
Q9D0D5	2.9e-08	25.42	General transcription factor IIE subunit 1 OS=Mus musculus OX=10090 GN=Gtf2e1 PE...	[more]
P36100	3.6e-06	20.12	Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (s...	[more]
Q9P3W1	4.7e-06	22.42	Transcription initiation factor IIE subunit alpha OS=Schizosaccharomyces pombe (...	[more]

BLAST of Cp4.1LG04g14280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531309.1	0.0	97.89	general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006507.1	0.0	97.67	general transcription factor IIE subunit 1-like [Cucurbita maxima]	[more]
XP_022933237.1	0.0	97.25	general transcription factor IIE subunit 1-like [Cucurbita moschata] >KAG6587625...	[more]
XP_038880289.1	2.99e-293	90.99	general transcription factor IIE subunit 1 isoform X1 [Benincasa hispida]	[more]
XP_038880290.1	1.59e-290	90.78	general transcription factor IIE subunit 1 isoform X2 [Benincasa hispida]	[more]

BLAST of Cp4.1LG04g14280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KW18	0.0	97.67	Transcription initiation factor IIE subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1EZ78	0.0	97.25	Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1BZ86	4.55e-288	90.81	Transcription initiation factor IIE subunit alpha OS=Momordica charantia OX=3673...	[more]
A0A6J1E5J2	1.35e-287	89.98	Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A5D3DYI4	2.84e-286	89.75	Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX...	[more]

BLAST of Cp4.1LG04g14280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03280.1	7.3e-156	64.73	Transcription factor TFIIE, alpha subunit	[more]
AT4G20340.1	4.5e-129	54.58	Transcription factor TFIIE, alpha subunit	[more]
AT4G20810.1	9.7e-108	50.33	transcription initiation factor IIE (TFIIE) alpha subunit family protein / gener...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 59..79
None	No IPR available	COILS	Coil	Coil	coord: 220..240
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 388..409
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 447..464
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 388..509
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 472..489
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 147..200
IPR002853	Transcription initiation factor IIE subunit alpha, N-terminal	SMART	SM00531	tfiie3	coord: 38..209 e-value: 1.0E-61 score: 221.0
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 147..206 e-value: 1.6E-8 score: 35.9
IPR024550	TFIIEalpha/SarR/Rpc3 HTH domain	PFAM	PF02002	TFIIE_alpha	coord: 40..93 e-value: 3.7E-7 score: 30.0
IPR039997	Transcription factor E	PANTHER	PTHR13097	TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT	coord: 5..449
IPR017919	Transcription factor TFE/TFIIEalpha HTH domain	PROSITE	PS51344	HTH_TFE_IIE	coord: 6..138 score: 34.170559
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 42..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g14280.1	Cp4.1LG04g14280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001113	transcription open complex formation at RNA polymerase II promoter
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
cellular_component	GO:0005673	transcription factor TFIIE complex