Cp4.1LG04g14180 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g14180
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	protein MIS12 homolog
Location	Cp4.1LG04: 11458832 .. 11462196 (+)
RNA-Seq Expression	Cp4.1LG04g14180
Synteny	Cp4.1LG04g14180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGACAAAAGATGATTTTTTGCTTTTAATTTTATAGCATTGTTTAAATTTTTAATCCATACAAGACATTTTCCGGTTCAGAATACAAGTCCGGTCCGAGTCCCCATCACTCCCCACTACCATTTTGGCGGCAAAATTCCTCCATTTGTGCGAAAGCTTGGGAGCTTCCATTTGCTCTCGATTTAAATTTCTTCTCTAGTTTTTCATTGGGAAAGATGCATTTTTGAGGAAGGGAAAGGCATTTTTTCATGGAAGAAAGTAAGGGCGAGGTGGTATTCGATTCGCTGAATCTGGATCCACATCTCTTCATTAATGAAGCTCTCAACGTTGTTGACGATTTGGTTGGTGATGCTTTCGATTTCTATCAATCGTATGCTTCTCTCTGTCTCGTCTCTTCGATTTGGATTTACAATTTTGTTCGGTTAAGTTTAGTCCTGTGAATTCCTTTTCCCTTCAGACAAGCATCAGCCGCCTTGAAGACCGAGGGCTCGGATAGATCCCAGGATCTTATAATGGTAAGGTTTTGGTTATGTTACTTGACAGTTTCTTATCAGTCGTTAATTTCCGATTTCTTAAGTTGTTACTCTGAAGCACTACTTCGAAATTGTTCCGGTTGTCTTTGAATTAAATTGTTTATTCTACCTAGGGAATATCGCGGGTCCGAACTCTCGTCCAATCGGGTCTGGATAAGCGGTTAGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGTGGGTTCTTCTACTCTGTCTGTGAAGAGGCTCCTGCTATTTTTATTTGGCGAAATTGCTCTATTATTGTTGTTTTTGTAACTGAATTCATTTCACAAGTGATTAATTGAGCATGTAAAAATGACTGTTTGCTTATGCAGCGAGTTTCCCACTTCTAATGTATATATGTGTACTGGCTTGGGAGCTTACTCAATTTTTGGTTCTGGGACGAACATATTTTGAAATGTTGGAAAAGCTTACTCAATTTATGCATCATCAAGTTTAATTTAGATCTAAATCTTTTCTTTAAGTTGTCGTAGGATGAATCTCCTGCTGACACTTCGATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGGTATTGAGTGCTTCATATGTTGATTACTTCTTGTGTTTCTGTAATTTCATAAGAAAATATATTTTTCAGGTCAGAAAAGAGAATGTTGAACTGAACCTGGAATTCCTAGTTTTGGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCAAGGGTAATTAAGTTATGCTTAGAATCGATTGTTTCTTCATGCACTCCTGACCATACATGCGCCATTATCCTATTAGTATACTCATGAGGGGGAAGACTCGAAATGAAATATACAAATAATGTGTGGAGCTGATTTATTTTGTTTTTCTTGTAATAAATTTGTATCGTCATAGCCCGCTTGGTAGATTAATTTTTTTGCAGACGCTGACTTTCTCCTCTGATTGATCATCCTCGTCTGCATAACCACGTGATACAAACTCTAATACATCCAAATAATATTTTAGTGAAGACCTGCACATTCCTTTGTTTCTGGTTTTGTTTAGGCGACTTTATTTTGCATCTAGATTTTCACATCGATCAAGGAGGGAAACGAAATATTCCTTATAAGGGTGTGAACCTCTCCTCAGCAGATGCGTTTTAAAACCGTGAGGTTGGTGGCGATACGACCAAAGAGGACAATATTTGTTAGGGGTAGACTTGGACCATTACATGATATTTCTTTTTGTTTTCTACGGGGTTCATCCGAGGAACGCTAGTCCGTGAATAATGCAATTTCTCTCGGTGTTTTCATTTTGAAAGCCAGTTGAAACACAATTTATTCATTAGCATTAAATTTCATTATACCTAAATTTAAACAGTTGGCAGGTAAAAGATGTTCATGATTTTTGGAATCAGAATTCTAACAAATATCAAATTCTTGCAGAAATGATGGGAACTGCATCGGACCTGCGAGCGAAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTATGATTTTCATTTTTTCAAGCTACTTCAGCATCAATAAGCTTGTTTTAGCTTTGTGGAAGGTGAATATCATCTAACATCCTATTTAATGCTTGTTTCTATTCTCAGGTTTCTCTAATGCTAAGCTGGATGACATTCCAGAAATTTTAGCCTATTTGAACCTCTGAACTTCATTATGACATCAACCTTACTGTATATTATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCAATGTTTTAACTTGATTTAGCTGTGAGATATTCTAATCTGTATAAATATATCATTTGGACTGATGAGTCTCCTTAATAGTCTCCATAGAGAATTTGCAGATGGGCCATGAACAGGCGTTGCCTCCCCAGATAGATCCGTACATATTTTCTCATGAAATTGAATTGCAGTTCATATTATTTTGGAACAGCTGGTGTAGATATCAAAAGGAATCATTTCTGAGGCTCTTGAACAAAAGTAAGCCTAAACTACCAACTGCAAGCGTTAGTAAAAGTGGGTTTCTGATGGATTCAAAATCAGCAATCTTCCACATCCCCTCGTTGTCAGCTTTGCCCCGATGACTCCTCTCGCTCGAGTACTCCCGGAGAAGATTGCTCAAATTGTCAACTTGGAGGATAATTTGACGCTGCGCCCGTGCTATATTTTGTATCTGGAAAATGTGAATGGAAAGCACAAAAATCATACTTCTAAGTTTGAATCTTAAAACCAAAGAAGAAAAGAAGTCATACTCATCAAAATACCTCTTCTAGCAGGGGAGATTCCTTAGCCAATTGGGAGGACGACAAAGAATGAGATAGCAGCGATCCAGTTAAGGCACCATTACTCAAGCCAGATATGAAAATTGAAGTTGCCCCAGAGCCGTTGTTGCTCGCATCGGCTTGTGAAGACAAGTTCTGCTGGCTAGCAGAAACGTTTCTAGTAGAGATCTTGGAGTTCAGCTCATCAATACGAGTTGTGAACTCATCCATCCTCTCGCTGAGAGTAGAAATCTGTTCTGAAAGTTGTGTAATGGCTCCCTGAATGAATTTTGAGATGGATCAGCAAATTAAAAAGCTGAGGACTTCCTCGACTCATTCAGCAATACTGTTAACCAAGAAACTATATGAATGTTTTATCTCTTCTTTGGGTCAAAGTCTTTACCAACACGTTCAGCCT

mRNA sequence

AAGGACAAAAGATGATTTTTTGCTTTTAATTTTATAGCATTGTTTAAATTTTTAATCCATACAAGACATTTTCCGGTTCAGAATACAAGTCCGGTCCGAGTCCCCATCACTCCCCACTACCATTTTGGCGGCAAAATTCCTCCATTTGTGCGAAAGCTTGGGAGCTTCCATTTGCTCTCGATTTAAATTTCTTCTCTAGTTTTTCATTGGGAAAGATGCATTTTTGAGGAAGGGAAAGGCATTTTTTCATGGAAGAAAGTAAGGGCGAGGTGGTATTCGATTCGCTGAATCTGGATCCACATCTCTTCATTAATGAAGCTCTCAACGTTGTTGACGATTTGGTTGGTGATGCTTTCGATTTCTATCAATCACAAGCATCAGCCGCCTTGAAGACCGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGTCCAATCGGGTCTGGATAAGCGGTTAGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGATGAATCTCCTGCTGACACTTCGATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGGTCAGAAAAGAGAATGTTGAACTGAACCTGGAATTCCTAGTTTTGGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCAAGGAAATGATGGGAACTGCATCGGACCTGCGAGCGAAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCCAGAAATTTTAGCCTATTTGAACCTCTGAACTTCATTATGACATCAACCTTACTGTATATTATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCAATGTTTTAACTTGATTTAGCTGTGAGATATTCTAATCTGTATAAATATATCATTTGGACTGATGAGTCTCCTTAATAGTCTCCATAGAGAATTTGCAGATGGGCCATGAACAGGCGTTGCCTCCCCAGATAGATCCGTACATATTTTCTCATGAAATTGAATTGCAGTTCATATTATTTTGGAACAGCTGGTGTAGATATCAAAAGGAATCATTTCTGAGGCTCTTGAACAAAAGTAAGCCTAAACTACCAACTGCAAGCGTTAGTAAAAGTGGGTTTCTGATGGATTCAAAATCAGCAATCTTCCACATCCCCTCGTTGTCAGCTTTGCCCCGATGACTCCTCTCGCTCGAGTACTCCCGGAGAAGATTGCTCAAATTGTCAACTTGGAGGATAATTTGACGCTGCGCCCGTGCTATATTTTGTATCTGGAAAATGTGAATGGAAAGCACAAAAATCATACTTCTAAGTTTGAATCTTAAAACCAAAGAAGAAAAGAAGTCATACTCATCAAAATACCTCTTCTAGCAGGGGAGATTCCTTAGCCAATTGGGAGGACGACAAAGAATGAGATAGCAGCGATCCAGTTAAGGCACCATTACTCAAGCCAGATATGAAAATTGAAGTTGCCCCAGAGCCGTTGTTGCTCGCATCGGCTTGTGAAGACAAGTTCTGCTGGCTAGCAGAAACGTTTCTAGTAGAGATCTTGGAGTTCAGCTCATCAATACGAGTTGTGAACTCATCCATCCTCTCGCTGAGAGTAGAAATCTGTTCTGAAAGTTGTGTAATGGCTCCCTGAATGAATTTTGAGATGGATCAGCAAATTAAAAAGCTGAGGACTTCCTCGACTCATTCAGCAATACTGTTAACCAAGAAACTATATGAATGTTTTATCTCTTCTTTGGGTCAAAGTCTTTACCAACACGTTCAGCCT

Coding sequence (CDS)

ATGGAAGAAAGTAAGGGCGAGGTGGTATTCGATTCGCTGAATCTGGATCCACATCTCTTCATTAATGAAGCTCTCAACGTTGTTGACGATTTGGTTGGTGATGCTTTCGATTTCTATCAATCACAAGCATCAGCCGCCTTGAAGACCGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGTCCAATCGGGTCTGGATAAGCGGTTAGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGATGAATCTCCTGCTGACACTTCGATAAGTTATGGCTGCCTCTATGATGTGGATCTGGATACAGAGCTAGATTCTTTAAGGAATAAGCTCTCGGAGGTCAGAAAAGAGAATGTTGAACTGAACCTGGAATTCCTAGTTTTGGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCAAGGAAATGATGGGAACTGCATCGGACCTGCGAGCGAAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCCAGAAATTTTAGCCTATTTGAACCTCTGA

Protein sequence

MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIMGISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLDTELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEMMGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL

Homology

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BLAST of Cp4.1LG04g14180 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.2e-50
Identity = 116/244 (47.54%), Postives = 160/244 (65.57%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSD--RSQDL 60
           ME SK E VFDS+NL+P +FINEA+N V+D V  AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVD 120
             GI+RVR L+ S +D RL +WE Y L  CF+VP+ F LP ++ES   +S+    LYD++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFK 180
           LD ELDSLR+KL+ V K +VEL+ E   LER + S        NEAL+LY++SS++E+FK
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EMMGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFS----NAKLDDIPEI 239
           EM   AS+LRA + +LK R+M+ S+   V +      + +H K FS    + KL+D+ + 
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 235

[Close]

BLAST of Cp4.1LG04g14180 vs. NCBI nr
Match: XP_023530578.1 (protein MIS12 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468 bits (1205), Expect = 2.87e-166
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM
Sbjct: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD
Sbjct: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM
Sbjct: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL 240
           MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL
Sbjct: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL 240

[Close]

BLAST of Cp4.1LG04g14180 vs. NCBI nr
Match: XP_022931718.1 (protein MIS12 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 437 bits (1125), Expect = 4.50e-154
Identity = 225/240 (93.75%), Postives = 229/240 (95.42%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SK +VVFDSLNLDP LFINEALNVVDDLVGDA DFYQSQAS ALK+EGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTL QSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELDSLRNKLSEVRKENVELN EF  LERKTASSNSQANYFNEALQLYEQSSVNEMF+EM
Sbjct: 121 TELDSLRNKLSEVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL 240
           MGTASDLRA+IGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDI E LAYLNL
Sbjct: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 240

[Close]

BLAST of Cp4.1LG04g14180 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 391 bits (1005), Expect = 9.00e-136
Identity = 201/238 (84.45%), Postives = 216/238 (90.76%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ+QASA+LKTEGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT VQSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELD LRNKLSEVRKENV LN E   LER+TASSNSQ N FNEALQLYEQ+SVNEMF+EM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           MGTAS+LRAKIGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDI E LA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Cp4.1LG04g14180 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389 bits (999), Expect = 7.38e-135
Identity = 200/238 (84.03%), Postives = 215/238 (90.34%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ+QASA+LKTEGSDRSQDL +
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT VQSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELD LRNKLSEVRKENV LN E   LER+TASSNSQ N FNEALQLYEQ+SVNEMF+EM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           MGTAS+LRAKIGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDI E LA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Cp4.1LG04g14180 vs. NCBI nr
Match: KAG7023263.1 (Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 388 bits (996), Expect = 2.11e-134
Identity = 199/238 (83.61%), Postives = 215/238 (90.34%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ+QASA+LKTEGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT VQSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELD LRNKLSEVRKENV LN E   LER+ ASSNSQ N FNEALQLYE++SVNEMF+EM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           MGTAS+LRAKIGKLK+R+ME+SKLT V+K+HTNGDISHHHKGFSNAKLDDI E LA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Cp4.1LG04g14180 vs. ExPASy TrEMBL
Match: A0A6J1EZI4 (protein MIS12 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437868 PE=3 SV=1)

HSP 1 Score: 437 bits (1125), Expect = 2.18e-154
Identity = 225/240 (93.75%), Postives = 229/240 (95.42%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SK +VVFDSLNLDP LFINEALNVVDDLVGDA DFYQSQAS ALK+EGSDRSQDLIM
Sbjct: 1   MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIM 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRTL QSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD
Sbjct: 61  GISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELDSLRNKLSEVRKENVELN EF  LERKTASSNSQANYFNEALQLYEQSSVNEMF+EM
Sbjct: 121 TELDSLRNKLSEVRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYLNL 240
           MGTASDLRA+IGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDI E LAYLNL
Sbjct: 181 MGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL 240

[Close]

BLAST of Cp4.1LG04g14180 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 385 bits (990), Expect = 8.39e-134
Identity = 197/238 (82.77%), Postives = 214/238 (89.92%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ+QASA+LKTEGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT VQSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELD LRNKLSEVRKEN+ LN E   LER+ ASSNSQ N FNEALQLYE++SVNEMF+EM
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           MGTAS+LRAKIGKLK+R+ME+SKLT V+K+HTNGD SHHHKGFSNAKLDDI E LA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Cp4.1LG04g14180 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 384 bits (985), Expect = 4.85e-133
Identity = 197/238 (82.77%), Postives = 213/238 (89.50%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ+QASA+LKTEGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT VQSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELD LR  LSEVRKEN+ LN E   LER+TASSNSQ N FNEALQLYEQ+SVNEMF+EM
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           MGTAS+LRAKIGKLK+R+ME+SKLT V+K+HTN DISHHHKGFSNAKLDDI E LA L
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Cp4.1LG04g14180 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 379 bits (973), Expect = 3.38e-131
Identity = 197/239 (82.43%), Postives = 213/239 (89.12%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ+QASA+LKTEGSDRSQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVRT VQSGLDKRLAMWEKYCLNHCFSVPE FSLPSNDESP  TS+S  CLYDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFK-E 180
           TELD LR  LSEVRKEN+ LN E   LER+TASSNSQ N FNEALQLYEQ+SVNEMF+ E
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQAE 180

Query: 181 MMGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           MMGTAS+LRAKIGKLK+R+ME+SKLT V+K+HTN DISHHHKGFSNAKLDDI E LA L
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADL 239

[Close]

BLAST of Cp4.1LG04g14180 vs. ExPASy TrEMBL
Match: A0A6J1C2Q6 (protein MIS12 homolog OS=Momordica charantia OX=3673 GN=LOC111006900 PE=3 SV=1)

HSP 1 Score: 374 bits (959), Expect = 4.42e-129
Identity = 191/238 (80.25%), Postives = 213/238 (89.50%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSDRSQDLIM 60
           ME SKGE VFDSLNL+P LFINEALN+VDDLV DAFDFYQ QASA LKTEG D+SQDLIM
Sbjct: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60

Query: 61  GISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVDLD 120
           GISRVR+ V S LDKRLAMWEKYCLNHCF+VPE FSLPSNDESPADTSIS+ CLYDV LD
Sbjct: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120

Query: 121 TELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFKEM 180
           TELDSLRN+LSEVR+ENV LN E   LER+TASSNSQ N+FNEAL+LYE++SVNE F+E+
Sbjct: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180

Query: 181 MGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIPEILAYL 238
           M TAS+LRAKIGKLK+R++E+SKLT V+KVHTNGD+SHHHKGFSNAKLDDI E LA L
Sbjct: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADL 238

[Close]

BLAST of Cp4.1LG04g14180 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-51
Identity = 116/244 (47.54%), Postives = 160/244 (65.57%), Query Frame = 0


Query: 1   MEESKGEVVFDSLNLDPHLFINEALNVVDDLVGDAFDFYQSQASAALKTEGSD--RSQDL 60
           ME SK E VFDS+NL+P +FINEA+N V+D V  AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRTLVQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNDESPADTSISYGCLYDVD 120
             GI+RVR L+ S +D RL +WE Y L  CF+VP+ F LP ++ES   +S+    LYD++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNKLSEVRKENVELNLEFLVLERKTASSNSQANYFNEALQLYEQSSVNEMFK 180
           LD ELDSLR+KL+ V K +VEL+ E   LER + S        NEAL+LY++SS++E+FK
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EMMGTASDLRAKIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFS----NAKLDDIPEI 239
           EM   AS+LRA + +LK R+M+ S+   V +      + +H K FS    + KL+D+ + 
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 235

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g14180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	4.2e-50	47.54	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Cp4.1LG04g14180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530578.1	2.87e-166	100.00	protein MIS12 homolog [Cucurbita pepo subsp. pepo]	[more]
XP_022931718.1	4.50e-154	93.75	protein MIS12 homolog isoform X1 [Cucurbita moschata]	[more]
KAG6589575.1	9.00e-136	84.45	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515499.1	7.38e-135	84.03	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG7023263.1	2.11e-134	83.61	Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cp4.1LG04g14180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EZI4	2.18e-154	93.75	protein MIS12 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437868 P...	[more]
A0A6J1E0A1	8.39e-134	82.77	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	4.85e-133	82.77	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1JD43	3.38e-131	82.43	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1C2Q6	4.42e-129	80.25	protein MIS12 homolog OS=Momordica charantia OX=3673 GN=LOC111006900 PE=3 SV=1	[more]

BLAST of Cp4.1LG04g14180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	3.0e-51	47.54	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 116..150
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 10..141 e-value: 2.4E-19 score: 69.8
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..233

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g14180.1	Cp4.1LG04g14180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus