Cp4.1LG04g12510 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g12510
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAmino acid permease family protein
LocationCp4.1LG04: 8017665 .. 8019110 (+)
RNA-Seq ExpressionCp4.1LG04g12510
SyntenyCp4.1LG04g12510
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGACAAAAAACAATCCCCGAACAAGTCCACCAACTCCCACCCCCTTGCTGATTCTTCTTCCCCTTCTCAAACCCTCCCCACCACCGCCGCCACCGCCACCTCCTCCGCCGCCGCCAGAAAGAAGCTCACTCTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGCGGCCCCTATGGCGAAGAGCCGGCTGTTCAAGCTGCCGGCCCTCTCCTCGCCATCGTTGGCTTCATCGTTTTCCCTTTCATATGGAGTGTCCCAGAGGCCCTAATCACGGCGGAGCTTTCCACCGCCTTCCCCGGAGACGGCGGTTTCGTTATCTGGGCTGACAGAGCCTTCGGACCCTTCTGGGGATCTTTAATGGGTACCTGGAAACTCCTCAGCGGCGTCATCAATATCGCTGCCTTCCCTGTTCTCTGCATCGATTACGTCAAGAAAATCGTTCCGCCGCTCGAATCTGGCTGGCCCCGCCGCATCGCCGTCTTCAGCTCCACCCTTCTCCTCGCCCTCCTTAACTACGTTGGCCTCACCATCGTCGGATACGTCGCCGTCGTCTTAGCCTTCTTGTCTCTCTTACCCTTTATTTTAATGACGTTCATCGCTATCCCCAAAATTCACCCCCACCGGTGGCTCAGTTTCGGCGACAAAGGCGTCAAAAAAGACTGGAATCTCTACCTCAATACTCTGTTTTGGAACCTCAATTTCTGGGATAATGTCTCCACTCTCGCCGGAGAAGTCGAAAACCCACAAAAAACATTCCCAATGGCTCTGTTTGTATCCGTGATTTTCACTTGTGTTTCTTATCTTATCCCACTTCTGGCAGTCATCGGCGCCGTCGATGTGGAACAATCCGCTTGGGGGTCTGGATTTCACGCACAAGCCGCCGGAATCATCGCCGGAAAATGGCTCAAAATCCTCCTGGAAATCGGGTCGTGCTTGTCGGGGATTGGATTATTCGAAGCCCAATTGAGTAGCAGTGCGTATCAGATTCTGGGTATGGCAGAAATCGGGATTTTGCCCAAATTCTTCGCGAGCAGAGCCAAATGGTTCAACACTCCATGGATTGGGATTGTGATCTGCACGGCGATTTCGCTGGCGGTGTCGTACATGAACTTCACCGACATTGTTGCGTCGGCGAATTTCATATACAGCTTGGGAATGTTGCTGGAATTTTCGGCGTTCATTTGGTTGAGATGGAAGCATCCGGGGATCAAAAGGCCATTCCAGGTGCCGTTGAGGTTGCCGGCGTTGATTGTTATGTGTTTGATTCCGTCGGCGTTTCTGGTGGTTCTGATGGTTTTCACGCACAAAATGGTGCTCGTGGTGAGTGCTATAATGACGGCAGCCGGGATTGTCTGGTTTGGGCTCATGAAGGTTTGTAAGAACAAGAAGATCTTGGAGTTCAATCCTCCGCTTGACGCCATTGAAGAATCCTGA

mRNA sequence

ATGGCGGACAAAAAACAATCCCCGAACAAGTCCACCAACTCCCACCCCCTTGCTGATTCTTCTTCCCCTTCTCAAACCCTCCCCACCACCGCCGCCACCGCCACCTCCTCCGCCGCCGCCAGAAAGAAGCTCACTCTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGCGGCCCCTATGGCGAAGAGCCGGCTGTTCAAGCTGCCGGCCCTCTCCTCGCCATCGTTGGCTTCATCGTTTTCCCTTTCATATGGAGTGTCCCAGAGGCCCTAATCACGGCGGAGCTTTCCACCGCCTTCCCCGGAGACGGCGGTTTCGTTATCTGGGCTGACAGAGCCTTCGGACCCTTCTGGGGATCTTTAATGGGTACCTGGAAACTCCTCAGCGGCGTCATCAATATCGCTGCCTTCCCTGTTCTCTGCATCGATTACGTCAAGAAAATCGTTCCGCCGCTCGAATCTGGCTGGCCCCGCCGCATCGCCGTCTTCAGCTCCACCCTTCTCCTCGCCCTCCTTAACTACGTTGGCCTCACCATCGTCGGATACGTCGCCGTCGTCTTAGCCTTCTTGTCTCTCTTACCCTTTATTTTAATGACGTTCATCGCTATCCCCAAAATTCACCCCCACCGGTGGCTCAGTTTCGGCGACAAAGGCGTCAAAAAAGACTGGAATCTCTACCTCAATACTCTGTTTTGGAACCTCAATTTCTGGGATAATGTCTCCACTCTCGCCGGAGAAGTCGAAAACCCACAAAAAACATTCCCAATGGCTCTGTTTGTATCCGTGATTTTCACTTGTGTTTCTTATCTTATCCCACTTCTGGCAGTCATCGGCGCCGTCGATGTGGAACAATCCGCTTGGGGGTCTGGATTTCACGCACAAGCCGCCGGAATCATCGCCGGAAAATGGCTCAAAATCCTCCTGGAAATCGGGTCGTGCTTGTCGGGGATTGGATTATTCGAAGCCCAATTGAGTAGCAGTGCGTATCAGATTCTGGGTATGGCAGAAATCGGGATTTTGCCCAAATTCTTCGCGAGCAGAGCCAAATGGTTCAACACTCCATGGATTGGGATTGTGATCTGCACGGCGATTTCGCTGGCGGTGTCGTACATGAACTTCACCGACATTGTTGCGTCGGCGAATTTCATATACAGCTTGGGAATGTTGCTGGAATTTTCGGCGTTCATTTGGTTGAGATGGAAGCATCCGGGGATCAAAAGGCCATTCCAGGTGCCGTTGAGGTTGCCGGCGTTGATTGTTATGTGTTTGATTCCGTCGGCGTTTCTGGTGGTTCTGATGGTTTTCACGCACAAAATGGTGCTCGTGGTGAGTGCTATAATGACGGCAGCCGGGATTGTCTGGTTTGGGCTCATGAAGGTTTGTAAGAACAAGAAGATCTTGGAGTTCAATCCTCCGCTTGACGCCATTGAAGAATCCTGA

Coding sequence (CDS)

ATGGCGGACAAAAAACAATCCCCGAACAAGTCCACCAACTCCCACCCCCTTGCTGATTCTTCTTCCCCTTCTCAAACCCTCCCCACCACCGCCGCCACCGCCACCTCCTCCGCCGCCGCCAGAAAGAAGCTCACTCTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGCGGCCCCTATGGCGAAGAGCCGGCTGTTCAAGCTGCCGGCCCTCTCCTCGCCATCGTTGGCTTCATCGTTTTCCCTTTCATATGGAGTGTCCCAGAGGCCCTAATCACGGCGGAGCTTTCCACCGCCTTCCCCGGAGACGGCGGTTTCGTTATCTGGGCTGACAGAGCCTTCGGACCCTTCTGGGGATCTTTAATGGGTACCTGGAAACTCCTCAGCGGCGTCATCAATATCGCTGCCTTCCCTGTTCTCTGCATCGATTACGTCAAGAAAATCGTTCCGCCGCTCGAATCTGGCTGGCCCCGCCGCATCGCCGTCTTCAGCTCCACCCTTCTCCTCGCCCTCCTTAACTACGTTGGCCTCACCATCGTCGGATACGTCGCCGTCGTCTTAGCCTTCTTGTCTCTCTTACCCTTTATTTTAATGACGTTCATCGCTATCCCCAAAATTCACCCCCACCGGTGGCTCAGTTTCGGCGACAAAGGCGTCAAAAAAGACTGGAATCTCTACCTCAATACTCTGTTTTGGAACCTCAATTTCTGGGATAATGTCTCCACTCTCGCCGGAGAAGTCGAAAACCCACAAAAAACATTCCCAATGGCTCTGTTTGTATCCGTGATTTTCACTTGTGTTTCTTATCTTATCCCACTTCTGGCAGTCATCGGCGCCGTCGATGTGGAACAATCCGCTTGGGGGTCTGGATTTCACGCACAAGCCGCCGGAATCATCGCCGGAAAATGGCTCAAAATCCTCCTGGAAATCGGGTCGTGCTTGTCGGGGATTGGATTATTCGAAGCCCAATTGAGTAGCAGTGCGTATCAGATTCTGGGTATGGCAGAAATCGGGATTTTGCCCAAATTCTTCGCGAGCAGAGCCAAATGGTTCAACACTCCATGGATTGGGATTGTGATCTGCACGGCGATTTCGCTGGCGGTGTCGTACATGAACTTCACCGACATTGTTGCGTCGGCGAATTTCATATACAGCTTGGGAATGTTGCTGGAATTTTCGGCGTTCATTTGGTTGAGATGGAAGCATCCGGGGATCAAAAGGCCATTCCAGGTGCCGTTGAGGTTGCCGGCGTTGATTGTTATGTGTTTGATTCCGTCGGCGTTTCTGGTGGTTCTGATGGTTTTCACGCACAAAATGGTGCTCGTGGTGAGTGCTATAATGACGGCAGCCGGGATTGTCTGGTTTGGGCTCATGAAGGTTTGTAAGAACAAGAAGATCTTGGAGTTCAATCCTCCGCTTGACGCCATTGAAGAATCCTGA

Protein sequence

MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEES
Homology
BLAST of Cp4.1LG04g12510 vs. ExPASy Swiss-Prot
Match: Q9LHN7 (Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g13620 PE=2 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 6.7e-180
Identity = 308/465 (66.24%), Postives = 383/465 (82.37%), Query Frame = 0

Query: 17  LADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLA 76
           ++++S  S  LP T A ++   A  KKLTLIPL+FLIYFEVAGGP+GEEPAVQAAGPLLA
Sbjct: 3   ISEASKSSHELPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLA 62

Query: 77  IVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINI 136
           I+GF++FPFIWS+PEALITAELSTAFPG+GGFVIWA RAFG F GS+MG+ K LSGVIN+
Sbjct: 63  ILGFLIFPFIWSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINV 122

Query: 137 AAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLP 196
           A+FPVLC+ Y+ K+ P LESGWPR + +F+ST++L+ LNY GL IVGY AVVL  +SL P
Sbjct: 123 ASFPVLCVTYLDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSP 182

Query: 197 FILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTF 256
           F++M+ +AIPKI PHRW S G K  KKDWNLY NTLFWNLNFWDNVSTLAGEV+ PQKTF
Sbjct: 183 FLVMSAMAIPKIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTF 242

Query: 257 PMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCL 316
           P+AL ++VIFTCV+YLIPL AV GAV V+QS W +GFHA+AA +IAGKWLKI +EIG+ L
Sbjct: 243 PLALLIAVIFTCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVL 302

Query: 317 SGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFT 376
           S IGLFEAQLSSSAYQ+ GMAE+G LPKFF  R+KWFNTPW+GI+I   +SL +SYMNFT
Sbjct: 303 SSIGLFEAQLSSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFT 362

Query: 377 DIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMV 436
           DI++SANF+Y+LGM LEF++FIWLR K P +KRP++VPL++P L+VMCLIPSAFLV+++V
Sbjct: 363 DIISSANFLYTLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILV 422

Query: 437 FTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEES 482
           F  K+V ++  +MT   I W+ L+   +  KI EFN  +D ++ +
Sbjct: 423 FATKIVYLICGVMTIGAIGWYFLINYFRKTKIFEFNEVIDDLDNN 465

BLAST of Cp4.1LG04g12510 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 3.2e-113
Identity = 219/476 (46.01%), Postives = 315/476 (66.18%), Query Frame = 0

Query: 7   SPN-KSTNSHPLADSSSPSQTLP------TTAATATSSAAA---RKKLTLIPLIFLIYFE 66
           SPN  S +  P   + +P    P      TT   ATS A      KK+T++PL+FLI++E
Sbjct: 6   SPNLDSASQKPRISTENPPPPPPHISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYE 65

Query: 67  VAGGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAF 126
           V+GGP+G E +V+AAGPLLAIVGFIVFPFIWS+PEALITAE+ T FP +GG+V+W   A 
Sbjct: 66  VSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAM 125

Query: 127 GPFWGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNY 186
           GP+WG   G  K LSGVI+ A +P+L +DY+K  +P L SG PR  A+   T+ L  LNY
Sbjct: 126 GPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNY 185

Query: 187 VGLTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNL 246
            GL+IVG  AV+L   S+LPF++M+F++IPK+ P RWL    K    +W+LYLNTLFWNL
Sbjct: 186 RGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNL 245

Query: 247 NFWDNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQ 306
           N+WD+VSTL GEVENP KT P ALF +++    SY+ P+L   GA+ ++Q  W  G+ A 
Sbjct: 246 NYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFAD 305

Query: 307 AAGIIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTP 366
              +I G WL   ++  +  S +G+F A++SS ++Q+LGMAE G+LP+ FA R++ + TP
Sbjct: 306 IGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTP 365

Query: 367 WIGIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLR 426
           W+GI+   +  + +S+++F +IVA+ N +Y  GM+LEF  F+ LR K+P   RPF++P+ 
Sbjct: 366 WVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVG 425

Query: 427 LPALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFN 473
           +   ++MC+ P+  + V+M FT+  V +VS      G+V    +K  + K  L+F+
Sbjct: 426 VLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFS 480

BLAST of Cp4.1LG04g12510 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.1e-112
Identity = 228/475 (48.00%), Postives = 311/475 (65.47%), Query Frame = 0

Query: 6   QSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEE 65
           +S   +  + P+ +  +  + LP   A     ++AR  +++IPLIFLI++EV+GGP+G E
Sbjct: 46  ESAQPANGAAPMGECGTEYRGLPDGDAGGPMPSSAR-TVSMIPLIFLIFYEVSGGPFGIE 105

Query: 66  PAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMG 125
            +V AAGPLLAI+GF+V P IWS+PEALITAEL   FP +GG+V+W   A GP+WG   G
Sbjct: 106 DSVGAAGPLLAIIGFLVLPVIWSIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQG 165

Query: 126 TWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYV 185
             K LSGVI+ A +PVL +DY+K  VP L  G PR  AV   T +L LLNY GLT+VG+V
Sbjct: 166 WMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWV 225

Query: 186 AVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTL 245
           A+ L   SLLPF +M  IA+PK+ P RWL      V  DWNLYLNTLFWNLN+WD++STL
Sbjct: 226 AICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSISTL 285

Query: 246 AGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKW 305
           AGEV+NP KT P ALF +VIF  V+YL PLLA  GAV +++  W  G+ A  A ++ G W
Sbjct: 286 AGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAW 345

Query: 306 LKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTA 365
           L   ++  + LS +G+F A++SS +YQ+LGMAE G+LP FFA+R++ + TP  GI+   +
Sbjct: 346 LMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSAS 405

Query: 366 ISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCL 425
             L +S M+F +IVA+ NF+Y  GMLLEF AFI  R + P   RP++VPL     + M +
Sbjct: 406 GVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLV 465

Query: 426 IPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEF--NPPLDAI 479
            P+A + V++  +   V VVS    A G+V    ++  + K+ L F  NP L  I
Sbjct: 466 PPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of Cp4.1LG04g12510 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.1e-112
Identity = 228/475 (48.00%), Postives = 311/475 (65.47%), Query Frame = 0

Query: 6   QSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEE 65
           +S   +  + P+ +  +  + LP   A     ++AR  +++IPLIFLI++EV+GGP+G E
Sbjct: 46  ESAQPANGAAPMGECGTEYRGLPDGDAGGPMPSSAR-TVSMIPLIFLIFYEVSGGPFGIE 105

Query: 66  PAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMG 125
            +V AAGPLLAI+GF+V P IWS+PEALITAEL   FP +GG+V+W   A GP+WG   G
Sbjct: 106 DSVGAAGPLLAIIGFLVLPVIWSIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQG 165

Query: 126 TWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYV 185
             K LSGVI+ A +PVL +DY+K  VP L  G PR  AV   T +L LLNY GLT+VG+V
Sbjct: 166 WMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWV 225

Query: 186 AVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTL 245
           A+ L   SLLPF +M  IA+PK+ P RWL      V  DWNLYLNTLFWNLN+WD++STL
Sbjct: 226 AICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSISTL 285

Query: 246 AGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKW 305
           AGEV+NP KT P ALF +VIF  V+YL PLLA  GAV +++  W  G+ A  A ++ G W
Sbjct: 286 AGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAW 345

Query: 306 LKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTA 365
           L   ++  + LS +G+F A++SS +YQ+LGMAE G+LP FFA+R++ + TP  GI+   +
Sbjct: 346 LMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSAS 405

Query: 366 ISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCL 425
             L +S M+F +IVA+ NF+Y  GMLLEF AFI  R + P   RP++VPL     + M +
Sbjct: 406 GVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLV 465

Query: 426 IPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEF--NPPLDAI 479
            P+A + V++  +   V VVS    A G+V    ++  + K+ L F  NP L  I
Sbjct: 466 PPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of Cp4.1LG04g12510 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 7.8e-112
Identity = 208/453 (45.92%), Postives = 308/453 (67.99%), Query Frame = 0

Query: 20  SSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIVG 79
           SS  +  +P++   AT      +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++G
Sbjct: 28  SSVGADEVPSSPPKATDKI---RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLG 87

Query: 80  FIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINIAAF 139
           F++FPFIWS+PEALITAE+ T +P +GG+V+W   A GPFWG   G  K LSGVI+ A +
Sbjct: 88  FVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALY 147

Query: 140 PVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLPFIL 199
           PVL +DY+K  VP L SG PR  ++   T+LL  LNY GLTIVG+VAV++   S+LPF +
Sbjct: 148 PVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAV 207

Query: 200 MTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMA 259
           M  I+IP++ P RWL   D G   +WNLYLNTLFWNLN+WD++STLAGEVENP  T P A
Sbjct: 208 MGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKA 267

Query: 260 LFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCLSGI 319
           LF  VI    SY+ PLLA IGA+ +E+  W  G+ +  A  + G WL+  ++  +  S +
Sbjct: 268 LFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNM 327

Query: 320 GLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFTDIV 379
           G+F A++SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  + +S+++F +IV
Sbjct: 328 GMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIV 387

Query: 380 ASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMVFTH 439
           A+ N +Y +GM+LEF AF+ +R KHP   RP+++P+     I+MC+ P+  +  ++  + 
Sbjct: 388 AAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSS 447

Query: 440 KMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFN 473
             V  VS +M   G +   L+     K+ ++F+
Sbjct: 448 LKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 474

BLAST of Cp4.1LG04g12510 vs. NCBI nr
Match: XP_023529220.1 (probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480

BLAST of Cp4.1LG04g12510 vs. NCBI nr
Match: XP_022933749.1 (probable polyamine transporter At3g13620 [Cucurbita moschata])

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 476/481 (98.96%), Postives = 479/481 (99.58%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MADKKQSPNKSTNS PLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAVFSSTLLLALLNYVGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFG+MK+CKNKKILEFNPPLDAIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAIEE 480

BLAST of Cp4.1LG04g12510 vs. NCBI nr
Match: KAG6588239.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022154.1 putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 927 bits (2395), Expect = 0.0
Identity = 472/481 (98.13%), Postives = 476/481 (98.96%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MADKKQS NKSTNS PLADSSSPSQTLPTT ATATSSAAARKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSLNKSTNSQPLADSSSPSQTLPTTVATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAVF STLLLALLNYVGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFGSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIPSAFLVVLMVFTHKMVLVVSA+MTAAGIVWFG+MK+CKNKKILEFNPPLDAIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGVMKICKNKKILEFNPPLDAIEE 480

BLAST of Cp4.1LG04g12510 vs. NCBI nr
Match: XP_023006107.1 (probable polyamine transporter At3g13620 [Cucurbita maxima])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 472/482 (97.93%), Postives = 477/482 (98.96%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAA-RKKLTLIPLIFLIYFEVAG 60
           MADKKQSPNKSTNS PLADSSS SQTLPTT+ATATSSAAA RKKLTLIPLIFLIYFEVAG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120
           GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGL 180
           WGSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAVFSSTLLLALLNYVGL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFW 240
           TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLS GDKGVKKDWNLYLNTLFWNLNFW
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300
           DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIG 360
           IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIG
Sbjct: 301 IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPWIG 360

Query: 361 IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA 420
           IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA
Sbjct: 361 IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA 420

Query: 421 LIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIE 480
           LI+MCLIPSAFLVVLMVFTHKMVLVVSA+MTAAGIVWFGLMKVCKNKKILEFNPPLDAIE
Sbjct: 421 LILMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDAIE 480

BLAST of Cp4.1LG04g12510 vs. NCBI nr
Match: XP_038878316.1 (probable polyamine transporter At3g13620 [Benincasa hispida])

HSP 1 Score: 807 bits (2085), Expect = 2.51e-292
Identity = 412/480 (85.83%), Postives = 441/480 (91.88%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MADKKQ    + NS+  A + S +Q LPTT  T  + AAARKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQQSLNNFNSNQPATADSSTQILPTT--TTPAPAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEPAVQAAGPLLAI+GFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KK+ PPLESGWPRRIA+ +STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKMAPPLESGWPRRIAILASTLILALLNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFI+IPKI PHRWL  GDK  K+DWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFISIPKIKPHRWLILGDK--KRDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAA  
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAET 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSSLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYM+F DIVASANFIYSLGMLLEFS+F+WLRWKHPG+KRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMDFADIVASANFIYSLGMLLEFSSFVWLRWKHPGMKRPFKVPLKLPGL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIPSAFLVV+MVFTH  V +VSA MT  GI+WFGLMK+CK KKILEFNP  +AI E
Sbjct: 421 IVMCLIPSAFLVVVMVFTHTNVFLVSAAMTVGGILWFGLMKICKKKKILEFNPETEAIVE 476

BLAST of Cp4.1LG04g12510 vs. ExPASy TrEMBL
Match: A0A6J1F0M1 (probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111441072 PE=3 SV=1)

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 476/481 (98.96%), Postives = 479/481 (99.58%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MADKKQSPNKSTNS PLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW
Sbjct: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAVFSSTLLLALLNYVGLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI
Sbjct: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL
Sbjct: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFG+MK+CKNKKILEFNPPLDAIEE
Sbjct: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGVMKICKNKKILEFNPPLDAIEE 480

BLAST of Cp4.1LG04g12510 vs. ExPASy TrEMBL
Match: A0A6J1KV06 (probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC111498946 PE=3 SV=1)

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 472/482 (97.93%), Postives = 477/482 (98.96%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAA-RKKLTLIPLIFLIYFEVAG 60
           MADKKQSPNKSTNS PLADSSS SQTLPTT+ATATSSAAA RKKLTLIPLIFLIYFEVAG
Sbjct: 1   MADKKQSPNKSTNSQPLADSSSTSQTLPTTSATATSSAAASRKKLTLIPLIFLIYFEVAG 60

Query: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120
           GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF
Sbjct: 61  GPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPF 120

Query: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGL 180
           WGSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAVFSSTLLLALLNYVGL
Sbjct: 121 WGSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVFSSTLLLALLNYVGL 180

Query: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFW 240
           TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLS GDKGVKKDWNLYLNTLFWNLNFW
Sbjct: 181 TIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSLGDKGVKKDWNLYLNTLFWNLNFW 240

Query: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300
           DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG
Sbjct: 241 DNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAG 300

Query: 301 IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIG 360
           IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFA+RAKWFNTPWIG
Sbjct: 301 IIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFATRAKWFNTPWIG 360

Query: 361 IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA 420
           IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA
Sbjct: 361 IVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPA 420

Query: 421 LIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIE 480
           LI+MCLIPSAFLVVLMVFTHKMVLVVSA+MTAAGIVWFGLMKVCKNKKILEFNPPLDAIE
Sbjct: 421 LILMCLIPSAFLVVLMVFTHKMVLVVSAVMTAAGIVWFGLMKVCKNKKILEFNPPLDAIE 480

BLAST of Cp4.1LG04g12510 vs. ExPASy TrEMBL
Match: A0A5A7UQT6 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00460 PE=3 SV=1)

HSP 1 Score: 798 bits (2061), Expect = 4.22e-289
Identity = 411/480 (85.62%), Postives = 439/480 (91.46%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MAD KQS   +++  P  DSSS  Q LPTT  T ++ AA RKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADDKQS---NSSLPPTTDSSS--QILPTT--TPSTPAAVRKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEP VQAAGPLLAIVGFI+FPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPTVQAAGPLLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAV +STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKI PHRWL  GDK  ++DWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFP ALFVSVIFTC+SYL+PLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIG+LPKFF SRAKWF TPWIGI
Sbjct: 301 IAGKWLKILLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYM+FTDIVASANFIYSLGMLLE S+F+WLRWKHPGIKRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMDFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPGL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIP+ FLVV+MVFTHK VL+VS  MT  GI+WFGLMK+CK KKILEFNP  +AI E
Sbjct: 421 IVMCLIPAGFLVVVMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPEAIVE 471

BLAST of Cp4.1LG04g12510 vs. ExPASy TrEMBL
Match: A0A1S3BNB6 (probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953 PE=3 SV=1)

HSP 1 Score: 798 bits (2061), Expect = 4.22e-289
Identity = 411/480 (85.62%), Postives = 439/480 (91.46%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MAD KQS   +++  P  DSSS  Q LPTT  T ++ AA RKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADDKQS---NSSLPPTTDSSS--QILPTT--TPSTPAAVRKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEP VQAAGPLLAIVGFI+FPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPTVQAAGPLLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAV +STL+LALLNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKI PHRWL  GDK  ++DWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFP ALFVSVIFTC+SYL+PLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLKILLEIGS LS IGLFEAQLSSSAYQILGMAEIG+LPKFF SRAKWF TPWIGI
Sbjct: 301 IAGKWLKILLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYM+FTDIVASANFIYSLGMLLE S+F+WLRWKHPGIKRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMDFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPGL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           IVMCLIP+ FLVV+MVFTHK VL+VS  MT  GI+WFGLMK+CK KKILEFNP  +AI E
Sbjct: 421 IVMCLIPAGFLVVVMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPEAIVE 471

BLAST of Cp4.1LG04g12510 vs. ExPASy TrEMBL
Match: A0A0A0M300 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 7.81e-288
Identity = 407/480 (84.79%), Postives = 436/480 (90.83%), Query Frame = 0

Query: 1   MADKKQSPNKSTNSHPLADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGG 60
           MAD KQ     +NS+  A + S SQ LPTT  T ++  + RKKLTLIPLIFLIYFEVAGG
Sbjct: 1   MADDKQQ----SNSNQPATTDSSSQILPTT--TPSTPPSVRKKLTLIPLIFLIYFEVAGG 60

Query: 61  PYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFW 120
           PYGEEP VQAAGPLLAI+GFIVFPFIWSVPEALITAELSTAFPG+GGFVIWA+RAFGPFW
Sbjct: 61  PYGEEPTVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFW 120

Query: 121 GSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLT 180
           GSLMGTWKLLSGVINIAAFPVLCIDY+KKI PPLESGWPRRIAV +STL+LA LNY+GLT
Sbjct: 121 GSLMGTWKLLSGVINIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILAALNYIGLT 180

Query: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWD 240
           IVGYVAVVLAFLSLLPFILMTFIAIPKI PHRWL  GDK  ++DWNLYLNTLFWNLNFWD
Sbjct: 181 IVGYVAVVLAFLSLLPFILMTFIAIPKIKPHRWLILGDK--ERDWNLYLNTLFWNLNFWD 240

Query: 241 NVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300
           NVSTLAGEVENPQKTFP ALFVSVIFTC+SYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI
Sbjct: 241 NVSTLAGEVENPQKTFPKALFVSVIFTCLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGI 300

Query: 301 IAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGI 360
           IAGKWLK LLEIGS LS IGLFEAQLSSSAYQILGMAEIG+LPKFF SRAKWF TPWIGI
Sbjct: 301 IAGKWLKFLLEIGSTLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGI 360

Query: 361 VICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPAL 420
           VICTAISLAVSYM+FTDIVASANFIYSLGMLLEFS+F+WLRW+HPGIKRPF+VPL+LP L
Sbjct: 361 VICTAISLAVSYMDFTDIVASANFIYSLGMLLEFSSFVWLRWRHPGIKRPFKVPLKLPGL 420

Query: 421 IVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEE 480
           I+MCLIPS FLVV+MVFTHK VL+VS  MT  GI+WFGLMK+CK KKILEFNP  +AI E
Sbjct: 421 IMMCLIPSGFLVVVMVFTHKNVLLVSGGMTVGGILWFGLMKICKKKKILEFNPEPEAIVE 472

BLAST of Cp4.1LG04g12510 vs. TAIR 10
Match: AT3G13620.1 (Amino acid permease family protein )

HSP 1 Score: 631.7 bits (1628), Expect = 4.8e-181
Identity = 308/465 (66.24%), Postives = 383/465 (82.37%), Query Frame = 0

Query: 17  LADSSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLA 76
           ++++S  S  LP T A ++   A  KKLTLIPL+FLIYFEVAGGP+GEEPAVQAAGPLLA
Sbjct: 3   ISEASKSSHELPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLA 62

Query: 77  IVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINI 136
           I+GF++FPFIWS+PEALITAELSTAFPG+GGFVIWA RAFG F GS+MG+ K LSGVIN+
Sbjct: 63  ILGFLIFPFIWSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINV 122

Query: 137 AAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLP 196
           A+FPVLC+ Y+ K+ P LESGWPR + +F+ST++L+ LNY GL IVGY AVVL  +SL P
Sbjct: 123 ASFPVLCVTYLDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSP 182

Query: 197 FILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTF 256
           F++M+ +AIPKI PHRW S G K  KKDWNLY NTLFWNLNFWDNVSTLAGEV+ PQKTF
Sbjct: 183 FLVMSAMAIPKIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTF 242

Query: 257 PMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCL 316
           P+AL ++VIFTCV+YLIPL AV GAV V+QS W +GFHA+AA +IAGKWLKI +EIG+ L
Sbjct: 243 PLALLIAVIFTCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVL 302

Query: 317 SGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFT 376
           S IGLFEAQLSSSAYQ+ GMAE+G LPKFF  R+KWFNTPW+GI+I   +SL +SYMNFT
Sbjct: 303 SSIGLFEAQLSSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFT 362

Query: 377 DIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMV 436
           DI++SANF+Y+LGM LEF++FIWLR K P +KRP++VPL++P L+VMCLIPSAFLV+++V
Sbjct: 363 DIISSANFLYTLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILV 422

Query: 437 FTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFNPPLDAIEES 482
           F  K+V ++  +MT   I W+ L+   +  KI EFN  +D ++ +
Sbjct: 423 FATKIVYLICGVMTIGAIGWYFLINYFRKTKIFEFNEVIDDLDNN 465

BLAST of Cp4.1LG04g12510 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 2.3e-114
Identity = 219/476 (46.01%), Postives = 315/476 (66.18%), Query Frame = 0

Query: 7   SPN-KSTNSHPLADSSSPSQTLP------TTAATATSSAAA---RKKLTLIPLIFLIYFE 66
           SPN  S +  P   + +P    P      TT   ATS A      KK+T++PL+FLI++E
Sbjct: 6   SPNLDSASQKPRISTENPPPPPPHISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYE 65

Query: 67  VAGGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAF 126
           V+GGP+G E +V+AAGPLLAIVGFIVFPFIWS+PEALITAE+ T FP +GG+V+W   A 
Sbjct: 66  VSGGPFGIEDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAM 125

Query: 127 GPFWGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNY 186
           GP+WG   G  K LSGVI+ A +P+L +DY+K  +P L SG PR  A+   T+ L  LNY
Sbjct: 126 GPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNY 185

Query: 187 VGLTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNL 246
            GL+IVG  AV+L   S+LPF++M+F++IPK+ P RWL    K    +W+LYLNTLFWNL
Sbjct: 186 RGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNL 245

Query: 247 NFWDNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQ 306
           N+WD+VSTL GEVENP KT P ALF +++    SY+ P+L   GA+ ++Q  W  G+ A 
Sbjct: 246 NYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFAD 305

Query: 307 AAGIIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTP 366
              +I G WL   ++  +  S +G+F A++SS ++Q+LGMAE G+LP+ FA R++ + TP
Sbjct: 306 IGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTP 365

Query: 367 WIGIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLR 426
           W+GI+   +  + +S+++F +IVA+ N +Y  GM+LEF  F+ LR K+P   RPF++P+ 
Sbjct: 366 WVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVG 425

Query: 427 LPALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFN 473
           +   ++MC+ P+  + V+M FT+  V +VS      G+V    +K  + K  L+F+
Sbjct: 426 VLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKKGWLKFS 480

BLAST of Cp4.1LG04g12510 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 405.6 bits (1041), Expect = 5.6e-113
Identity = 208/453 (45.92%), Postives = 308/453 (67.99%), Query Frame = 0

Query: 20  SSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIVG 79
           SS  +  +P++   AT      +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++G
Sbjct: 28  SSVGADEVPSSPPKATDKI---RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLG 87

Query: 80  FIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINIAAF 139
           F++FPFIWS+PEALITAE+ T +P +GG+V+W   A GPFWG   G  K LSGVI+ A +
Sbjct: 88  FVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALY 147

Query: 140 PVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLPFIL 199
           PVL +DY+K  VP L SG PR  ++   T+LL  LNY GLTIVG+VAV++   S+LPF +
Sbjct: 148 PVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAV 207

Query: 200 MTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMA 259
           M  I+IP++ P RWL   D G   +WNLYLNTLFWNLN+WD++STLAGEVENP  T P A
Sbjct: 208 MGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKA 267

Query: 260 LFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCLSGI 319
           LF  VI    SY+ PLLA IGA+ +E+  W  G+ +  A  + G WL+  ++  +  S +
Sbjct: 268 LFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNM 327

Query: 320 GLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFTDIV 379
           G+F A++SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  + +S+++F +IV
Sbjct: 328 GMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIV 387

Query: 380 ASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMVFTH 439
           A+ N +Y +GM+LEF AF+ +R KHP   RP+++P+     I+MC+ P+  +  ++  + 
Sbjct: 388 AAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSS 447

Query: 440 KMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFN 473
             V  VS +M   G +   L+     K+ ++F+
Sbjct: 448 LKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 474

BLAST of Cp4.1LG04g12510 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 405.6 bits (1041), Expect = 5.6e-113
Identity = 208/453 (45.92%), Postives = 308/453 (67.99%), Query Frame = 0

Query: 20  SSSPSQTLPTTAATATSSAAARKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIVG 79
           SS  +  +P++   AT      +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++G
Sbjct: 12  SSVGADEVPSSPPKATDKI---RKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLG 71

Query: 80  FIVFPFIWSVPEALITAELSTAFPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINIAAF 139
           F++FPFIWS+PEALITAE+ T +P +GG+V+W   A GPFWG   G  K LSGVI+ A +
Sbjct: 72  FVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALY 131

Query: 140 PVLCIDYVKKIVPPLESGWPRRIAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLPFIL 199
           PVL +DY+K  VP L SG PR  ++   T+LL  LNY GLTIVG+VAV++   S+LPF +
Sbjct: 132 PVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAV 191

Query: 200 MTFIAIPKIHPHRWLSFGDKGVKKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMA 259
           M  I+IP++ P RWL   D G   +WNLYLNTLFWNLN+WD++STLAGEVENP  T P A
Sbjct: 192 MGLISIPQLEPSRWLVM-DLG-NVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKA 251

Query: 260 LFVSVIFTCVSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCLSGI 319
           LF  VI    SY+ PLLA IGA+ +E+  W  G+ +  A  + G WL+  ++  +  S +
Sbjct: 252 LFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNM 311

Query: 320 GLFEAQLSSSAYQILGMAEIGILPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFTDIV 379
           G+F A++SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  + +S+++F +IV
Sbjct: 312 GMFIAEMSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIV 371

Query: 380 ASANFIYSLGMLLEFSAFIWLRWKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMVFTH 439
           A+ N +Y +GM+LEF AF+ +R KHP   RP+++P+     I+MC+ P+  +  ++  + 
Sbjct: 372 AAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSS 431

Query: 440 KMVLVVSAIMTAAGIVWFGLMKVCKNKKILEFN 473
             V  VS +M   G +   L+     K+ ++F+
Sbjct: 432 LKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFS 458

BLAST of Cp4.1LG04g12510 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 2.0e-110
Identity = 207/442 (46.83%), Postives = 302/442 (68.33%), Query Frame = 0

Query: 42  KKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIVGFIVFPFIWSVPEALITAELSTA 101
           +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++GF++FPFIW +PEALITAE+ST 
Sbjct: 34  QKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLALLGFVIFPFIWCIPEALITAEMSTM 93

Query: 102 FPGDGGFVIWADRAFGPFWGSLMGTWKLLSGVINIAAFPVLCIDYVKKIVPPLESGWPRR 161
           FP +GGFV+W   A G FWG  +G  K L GVI+ A +PVL +DY+K  VP L +G PR 
Sbjct: 94  FPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPALATGLPRV 153

Query: 162 IAVFSSTLLLALLNYVGLTIVGYVAVVLAFLSLLPFILMTFIAIPKIHPHRWLSFGDKGV 221
            ++   TLLL  LNY GLTIVG+ AV +   S+LPF +M+ ++IP++ P RWL   D G 
Sbjct: 154 ASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWLVM-DLG- 213

Query: 222 KKDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPMALFVSVIFTCVSYLIPLLAVIGA 281
             +WNLYLNTL WNLN+WD+VSTLAGEV NP+KT P AL   VIF  +S  +PLL+  GA
Sbjct: 214 NVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLLSGTGA 273

Query: 282 VDVEQSAWGSGFHAQAAGIIAGKWLKILLEIGSCLSGIGLFEAQLSSSAYQILGMAEIGI 341
           + +++  W  G+ A+ A  I G WL++ ++  +  S +G+F A++SS ++Q+LGMAE+GI
Sbjct: 274 IPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGMAELGI 333

Query: 342 LPKFFASRAKWFNTPWIGIVICTAISLAVSYMNFTDIVASANFIYSLGMLLEFSAFIWLR 401
           LP+ FA R++ + TP +GI+   +  L +S ++F +I+A+ N +Y  GM+LEF AF+ LR
Sbjct: 334 LPEIFAQRSR-YGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAFVRLR 393

Query: 402 WKHPGIKRPFQVPLRLPALIVMCLIPSAFLVVLMVFTHKMVLVVSAIMTAAGIVWFGLMK 461
            KHP   RP+++P+     I++C+ P   + +++V +   V +VS +M   G     LMK
Sbjct: 394 KKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGF----LMK 453

Query: 462 VCKN----KKILEFNPPLDAIE 480
            C N    KK ++F+   D  E
Sbjct: 454 PCLNHMDGKKWVKFSVCSDLAE 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN76.7e-18066.24Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9FFL13.2e-11346.01Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
A2X8M82.1e-11248.00Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D02.1e-11248.00Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Q9C6S57.8e-11245.92Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Match NameE-valueIdentityDescription
XP_023529220.10.0100.00probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo][more]
XP_022933749.10.098.96probable polyamine transporter At3g13620 [Cucurbita moschata][more]
KAG6588239.10.098.13putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] ... [more]
XP_023006107.10.097.93probable polyamine transporter At3g13620 [Cucurbita maxima][more]
XP_038878316.12.51e-29285.83probable polyamine transporter At3g13620 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1F0M10.098.96probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KV060.097.93probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A5A7UQT64.22e-28985.63Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BNB64.22e-28985.63probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953... [more]
A0A0A0M3007.81e-28884.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13620.14.8e-18166.24Amino acid permease family protein [more]
AT5G05630.12.3e-11446.01Amino acid permease family protein [more]
AT1G31830.15.6e-11345.92Amino acid permease family protein [more]
AT1G31830.25.6e-11345.92Amino acid permease family protein [more]
AT1G31820.12.0e-11046.83Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 25..479
e-value: 3.5E-62
score: 208.0
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 40..473
e-value: 5.9E-60
score: 205.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR45826:SF8BNAC05G39680D PROTEINcoord: 20..473
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 66..454
e-value: 2.5E-32
score: 112.3
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 20..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g12510.1Cp4.1LG04g12510.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity