Cp4.1LG04g10980 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g10980
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmitogen-activated protein kinase kinase 2-like
LocationCp4.1LG04: 9193846 .. 9200923 (+)
RNA-Seq ExpressionCp4.1LG04g10980
SyntenyCp4.1LG04g10980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGGGAGGCTTAAGCAACAATCTCGACCTCAAACTCAGTCTCCCTCAGGAAGACCAATCCATAGCCAAGTTCCTGTAATTTCTCTCTCTCTTTCGCTCTCTCTGTTTGTGTGTGTGAGTAATGGAATGTTCTGATGAATCTGGAATTTGGATTTGTTATTCTTGTGTAGAACGCAGAGCGGCACGTTTAAGGATGGTGATCTGCTTGTAAACAGAGATGGAGTTCGGATTGTTTCTCAGAGCGAAGTTGAAGCAGTAAGATATCTCAGTTTTAGAGCGTAGTATAGTATCTTCATTGGACGATAATGTGCTGTAGTTTTGATATTTTGCTTGCGGGTTTTAGGCTTTTAGCGTTTTAGCTTTTCGATTATGCGATTTCTTGTTACATAGGACGTCTTTGATTCTGTTTCTGTAGCATCAAGGTATTGATTATGGAATTATGAATCCTTATACTCGACCGTATGTGCTGTACTCATTTCAGTAACCAGGGAATGGATATCTAATTTGGCTTAGAGAGTTAGAGTTGTTACTATCTTCTCATCAACTAACGAATGAATGAAGTATATATATTCTTGAATATATGCTTACGTTATCTGCAAAATTTGGATTTCTCACGACTGACTTGTTCTTTAGGTTAGAACAAATGCAACCCAATTGTGACATCCCAGTTTTGCTTTGTTCGGGATGTGTTGTCCATGGTTGCATGCCCATTTCAAACCCCTTTTACATGCTTTTCTCTTAGATAGGTCTTTACCATTCCATATTGGGTCAGTATGAGGGTGTATGACCGTTCGCCAAAATCATGTCTACACCTGCGAGGGCTAAAATGCCCCAAAATGTACTATACGGGAATATGACTAGTTATCAACTTTTTCCTACCGATAGTTCTATGTAGTAAGTCGTTGTTGTTGCCTTTTTACTTACATTTCCATATAACACCATTTTCAATTTCTCAAATTTTACTTTCAAAACTCTCTTTCGAAATCTCTCTGTCCCCGTTTTCTAAAATAAAATTTCTTGAACCGAATGCAATTGTGACAAGTGGTATCACGTCAATATAATATTACAAAAAAGCGAAACAATTAATAAAAAAAATTAATATTTAAAAGTAACAAAACTAGTAATTTACAATTGTGTATAAAAGGAAAAAGCCAAAGCAAAATCGTCCAATATTAGTGAAACAAATATGGGGTTTTTGGTATCAGATAATCAAAAACAAAAATAAAGCCAAAACGAACCGTTTTATTGCGCAAAAGATATATTTTGAAGCGTAGCGGCCAACGCAATCCGTATCTTATCAAATATCACCAGCGACTGAACAATCACGCTCTATACGGGAAATCTGAACCGTACGTGGTACCACGTGAACTTATGGCGCGTGAGATGGGATTTTTGGTTCCTAAAGAGAGAGGTGACAGCGTGGAAAAAGTTGAACCACTGACAAGAAGAAGAATAGTGTGGGGCCCACCAAGTCTCAATCTAACGAAGGAGTGTCCCACTGGACAGCCTGTACCCTTTTTCTAATTTCCACATATATTTCTATTTATTTTTCCTTTTTTGACCCCTTTTTTTTTATTTACCCCTTTTTTATGTATAAATCAGAGTGGTTTTACCTTTTTTTTTTTTTTAACCTATTTTACCCAAGATTAAAGTTACTAGGTGAAATTCTATTCATTGATAGTGATTCAAATCAATTATATAAATAGATGAAAAGGGTAAATTCGTAATTTGACCTATCAACTAATTCAATAATTTTGATAACCAGAAACTCAAATATTACAATTTTTTTTTAATTATTTTTCATTTAAACTATTTTTAAAAAATAAGCTGCCTGTCACATTTAAAAAAAAATAATTGATATTTGGGCTCGTACATGGGCTGGGCTTGTGGCCCATTAGAACTCAGTCGACCACTGTCGACATTGCCGTCTTCGACACCCACACGGTTTCAATGTCGACTTTTGATATTTCGACGTGCAAAACTACGTCATTTTTATTTATAATTATGACGTCATAATTTTACGAAAAATCAATATTATAACTTTTTATTTATAAATTACAAAGATGGTAATTACATTATCCGCTAAATTATGTCTTAATAACTTTTTTATTCTAATATTTATCTCTATATTTTAAATAAATTTGATAATAAAAAAAAGGATAAGGCCCTTAAATAAATAATGGTCAAAATTTTGAAAACATTAAAAAAATGACAAAAATAAGTCAAATAGGTTTAGAAATTGGGATAATAATGACATGGAACGTAATTATATTTTTAAAATTTATTAATTATATCCCGATCACCGATCTTATGAGTCTATTTTTATTAACATTGTTAATTTGTACTATTTTGTACCTAGAAAGAGGAGGTAAAATGAGAGTTGAGTATGGTTACACGATGATTCCCAATTAAATTAAAATAATTTAATATATTTCAAAATTAAATTGAATTATGTTAAACTAGAAATGTTTTTATATTTTAATTTATGGACTAAAAAATATTTTTCCCTAATTGTTATTAATGTTGGTATATCTATCCATTTCAATTATACAGCTGGACAATTGAGCCATAAATTCATCATCATATATATATATATAAAGATGTGTTTGACGATTCTCTGAATTGAATCTCTCTCCCAGTCATCGAATCATCACAAGAAGCTTTGATTTGAGTATCGATTCTACGGGGGTTGGAGCCTGGAAGTGGAAGATATCCACCATCAATGAGGAAGGGAGGCTTAAGCAACAATCTCGACCTCAAACTCAGTCTCCCTCAGGAAGACCAATCCATAGCCAAGTTCCTGTAATTTCTCTCTCTCTTTCGCTCTCTCTGTTTGTGTGTGTGAGTAATGGAATGTTCTGATGAATCTGGAATTTGGATTTGTTATTCTTGTGTAGAACGCAGAGCGGCACGTTTAAGGATGGTGATCTGCTTGTAAACAGAGATGGAGTTCGGATTGTTTCTCAGAGCGAAGTTGAAGCAGTAAGATATCTCAGTTTTAGAGCGTAGTATAGTATCTTCATTGGACGATAATGTGCTGTAGTTTTGATATTTTGCTTGCGGGTTTTAGGCTTTTAGCGTTTTAGCTTTTCGATTATGCGATTTCTTGTTACATAGGACGTCTTTGATTCTGTTTCTGTAGCATCAAGGTATTGATTATGGAATTATGAATCCTTATACTCGACCGTATGTGCTGTACTCATTTCAGTAACCAGGGAATGGATATCTAATTTGGCTTAGAGAGTTAGAGTTGTTACTATCTTCTCATCAACTAACGAATGAATGAAGTATATATATGTAACAACTCAAGTCCATCGCTAGCGGATATTGTCCTCTTTGGCCCCTCAAGGTTTTTAAAATTCGTCTGCTAGGGAGAAGTTATAAAGAATGCCTCGTTCTTCTCCTCAGTCGATGTGGGATCTCACAATATCACACCAGATTTCTTCGTTAAACTTGTTTGTTTTTAGACACGGGGAGAAAATGGAAAAAGTGGTTGATTATGTTGTATTACTAACTATGTTGTGCGTAATGGGATACTAACTTGTTTTTTTTTGTCTGTCTACCTGTGCGTTCAGCCACCTCCGATTAAGCCTACAGATGATCAGTTGAGTTTAGCGGACATAGACACAATTAAAGTCATTGGAAAAGGAAATGGTGGAACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTATTGCACTGCACGGCTTAAACATTATCTTCTTCCCAAAATTTATGTGCTTCTGACTTGTGTGCCTCATAGACTATATAGTGTTGGTGGTTCAAAAAATGGCTAGATAGCATGATTTCTGTTTCAGTCTTTGTGATCTTCCAGTATCCTAATGATCATTATATTGGAAGTCTGATTTTTGAAGGACTAACTCCCCTTCATCTGTTTTTGCATTTTTCTGAATCTACCATATTCCTTAGCTACTTCAACATTGCTTTCAAATTATGTTTTTATCTCTGAGTTGTTTTAAGAAAGATCAAGTGCATATACATTTCAACAGAATCTAAGGATTTATTTCATTCCAAGGACTTTGACGCTATTTTTAGCAAGGTCTTATAATTTCATGGCAACTATAGCATTCGGTGATTTCTTTATAGAAGTTTAAGGACTTTAATAGATGGAATTTCTGGACATAACTTCATGTGAGCAAAAAGCTGTTTAATAAAAGAAGATATAATTTTCCTTCACAGTTTGAACCAAAAACCCAGTATCAAATCTACTCTACCATCTCAACATTTATGGAGTTGTTTTGGTGAACTAGTCAGTCAATGAGCGTTAAGACAAGCTGTTTCACACTCCTGTCTGTACTCTTCCCATTAGATATTTTAGAATCAAATTTTCAGCATTAATTAGCCCAAAACTAAGAAATCATCAATATGTTTTCATATCGTATCAGTTCCTATCCTCTTTCAGGTAATTGAGATGAAAATTGAGGAGTCTTACCGCAGGCAGATTGCTAAAGAACTCAAAATCAATCAATCAGCACAGTGTCCCTATATTGTTGTCTGTTACCAATCTTTCTATGATAATGGGTCAATATATATCATTTTTGAGTACATGGATGGTGGATCTTTAGCTGATTTTCTGAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCATTTGTAAGCAGGTAGGTGCAAAAAAGTCCAGCTTGGATATTGGGAATTTGTTCTTTTACTTCATTTACTTTTCCACTAAAGGAGATAATTGTAGACTTCCTTTTAACCCCTTTCCTCTCTTCTTCAGGTGCTGAAGGGGCTGATGTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCTAATTTATTAGTAAATCATAGAGGGGAAGTCAAGATTACCGACTTCGGTGTGAGTGCGATTATGGCAAGCACAACTGGAGAGGCTAATACTTTCGTTGGCACATATAACTATATGTCTGTGAGTATCAATTCTCAATATATTCACATAAATACAATGCTTTCGCTGTTTGTCTTCCTTTCATTTTTTCTGGTAAGAGCTGTACATTTTTTCCATCTATATAACCTATGTGTATAATAGCTTACTGGTGTGTCATTGTTATTTATAGCCAGAGAGAATTGTTGGAAATAGATATGGCAAAAAAAGTGACATTTGGAGCTTGGGGCTGATATTACTTGAATGTGCAACCGGCAAATTCCCTTATTCTCCACCCGAGAAAGATGGAGGATGGGGTAACTTTTATGATCTTATGGTAGCCATTGCTGAGGGCGAACCTCCTTCTGCTCCAGCCGACCAGTTTACTCCCAACTTCTGTTCCTTCATTTCTGCATGGTAAGGATCTGATTTTTCCTGGGGTCTTGACAGCCTAACACCAAACAACCCTTTTCTAAAGAATATGCCAGTTATTATATGAAATATGAGTAGGAATTTATATCTCTAAGTAGGCTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTGTACTCTAGCTTATTTCAATTCCTTTACGTTATGAGATTGCTCTTACCAAAAATAATTATATATAAAAAAAAAAAAAAAAACCGTTATGAGATTGCTAACTCAGACACTGATTATCTTTATGAATATCAGTTTGCAAACGGAGCCGAAGGATAGACTGTCAGCACGTGAACTTTTGGTATATATTTCTTCCTTCCACATGTTGATAAGAAAGATGTCAGCATAATAGAAAAGATAAATCTGAAATATTCTTCTCCTTTTGTGTATTTTCAGGAACACCCTTTCGTCAACATGTATGGAGATACAGATATTGACCTGTCATCTTACTTCAATGATGCAGGGACTCCACTTGCAACGTTCTAAATTTGATCTGGTATGATATGGAACTACATTCCATTTAAGACTTACTCTTGATATCTTTCTGTATCTGGTTGAGTGTAAGGCGATCTTTTCTTTGCGATGGAATCTATCATTTGGTGTAATTAATTCGGAATTTCTCGGATTTCATGTTTGCATGCCAAACATGTATTTAGCTATGTTTTCTCTTTTGGCCCTTCTATGATTTATTTCTGAAAACCAAGTATCTATCCCCCAATGCTAGTGAGAGATTCTTTAGCTCTGCCAGTTCACAATGTTGGGCATCTTGAATATTCAAATTTGATTCCCCAGCATAACAATGTGTATGATTTGTGTGTATATGCGATATGATTAAATCAAGGACGCCAGCTTTGTCCAGGACAAGTGGCTGAAGTAATATTTGTACAGCAGCATATGAGAAATGTATGAACTCGCTAGACGAGGTTTCAAAACCTGTGCATGAAAGCTGTTTAAAACTTGTATAATAGGTGAAAAGGGTTCAAAATTGATGAAAGTTAGAAACATGATAATGTCCCAAGATGGCCCACATAAGCTTCTCTATCTCTGTTTCTCGCTCTAGCTTAACCATTATCTGTTCATGCTAATGATGCATCATTTTTATTGTTTTCATTCATTCTCTGCCTCGTACACGATAATCTCAATTGTTGGCTGCTTTTAAGGCTTGCAGGGGATAGACAAGGATTACGCCGCCATCTCACATTCTCCTGAATGCTGCTTGTTTTACTTCAGTTTGTCTGATTGAAAGAGGTGTTTCAATCAGAACTGGATGATGGTTGTTCCTTTATTGGTCACATTTTAGATGGGGTTCTGATCCTCCTTGAGGATGATGATTCAGAAGGATACGGAAATGTTATGTTGTGCAAAGAGATTTCACATCGTGGCCTTTTCTTGTAAGATTTTGTAATCCCATTCTACTACTCACCATGTAAAATTTGGAGCATCAATGATATTAAATACATGTTATGACACAGAAGCACTGTGCATAAATTTTAATCCCAACAT

mRNA sequence

ATGAGGAAGGGAGGCTTAAGCAACAATCTCGACCTCAAACTCAGTCTCCCTCAGGAAGACCAATCCATAGCCAAGTTCCTAACGCAGAGCGGCACGTTTAAGGATGGTGATCTGCTTGTAAACAGAGATGGAGTTCGGATTGTTTCTCAGAGCGAAGTTGAAGCAGACGTCTTTGATTCTGTTTCTGTAGCATCAAGTCATCGAATCATCACAAGAAGCTTTGATTTGAGTATCGATTCTACGGGGGTTGGAGCCTGGAAGTGGAAGATATCCACCATCAATGAGGAAGGGAGGCTTAAGCAACAATCTCGACCTCAAACTCAGTCTCCCTCAGGAAGACCAATCCATAGCCAAGTTCCTAGCGGCACGTTTAAGGATGGTGATCTGCTTGTAAACAGAGATGGAGTTCGGATTGTTTCTCAGAGCGAAGTTGAAGCACCACCTCCGATTAAGCCTACAGATGATCAGTTGAGTTTAGCGGACATAGACACAATTAAAGTCATTGGAAAAGGAAATGGTGGAACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTAATTGAGATGAAAATTGAGGAGTCTTACCGCAGGCAGATTGCTAAAGAACTCAAAATCAATCAATCAGCACAGTGTCCCTATATTGTTGTCTGTTACCAATCTTTCTATGATAATGGGTCAATATATATCATTTTTGAGTACATGGATGGTGGATCTTTAGCTGATTTTCTGAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCATTTGTAAGCAGGTGCTGAAGGGGCTGATGTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCTAATTTATTAGTAAATCATAGAGGGGAAGTCAAGATTACCGACTTCGGTGTGAGTGCGATTATGGCAAGCACAACTGGAGAGGCTAATACTTTCGTTGGCACATATAACTATATGTCTCCAGAGAGAATTGTTGGAAATAGATATGGCAAAAAAAGTGACATTTGGAGCTTGGGGCTGATATTACTTGAATGTGCAACCGGCAAATTCCCTTATTCTCCACCCGAGAAAGATGGAGGATGGGGTAACTTTTATGATCTTATGGTAGCCATTGCTGAGGGCGAACCTCCTTCTGCTCCAGCCGACCAGTTTACTCCCAACTTCTGTTCCTTCATTTCTGCATGTTTGCAAACGGAGCCGAAGGATAGACTGTCAGCACGTGAACTTTTGGAACACCCTTTCGTCAACATGTATGGAGATACAGATATTGACCTGTCATCTTACTTCAATGATGCAGGGACTCCACTTGCAACGTTCTAAATTTGATCTGGCTTGCAGGGGATAGACAAGGATTACGCCGCCATCTCACATTCTCCTGAATGCTGCTTGTTTTACTTCAGTTTGTCTGATTGAAAGAGGTGTTTCAATCAGAACTGGATGATGGTTGTTCCTTTATTGGTCACATTTTAGATGGGGTTCTGATCCTCCTTGAGGATGATGATTCAGAAGGATACGGAAATGTTATGTTGTGCAAAGAGATTTCACATCGTGGCCTTTTCTTGTAAGATTTTGTAATCCCATTCTACTACTCACCATGTAAAATTTGGAGCATCAATGATATTAAATACATGTTATGACACAGAAGCACTGTGCATAAATTTTAATCCCAACAT

Coding sequence (CDS)

ATGAGGAAGGGAGGCTTAAGCAACAATCTCGACCTCAAACTCAGTCTCCCTCAGGAAGACCAATCCATAGCCAAGTTCCTAACGCAGAGCGGCACGTTTAAGGATGGTGATCTGCTTGTAAACAGAGATGGAGTTCGGATTGTTTCTCAGAGCGAAGTTGAAGCAGACGTCTTTGATTCTGTTTCTGTAGCATCAAGTCATCGAATCATCACAAGAAGCTTTGATTTGAGTATCGATTCTACGGGGGTTGGAGCCTGGAAGTGGAAGATATCCACCATCAATGAGGAAGGGAGGCTTAAGCAACAATCTCGACCTCAAACTCAGTCTCCCTCAGGAAGACCAATCCATAGCCAAGTTCCTAGCGGCACGTTTAAGGATGGTGATCTGCTTGTAAACAGAGATGGAGTTCGGATTGTTTCTCAGAGCGAAGTTGAAGCACCACCTCCGATTAAGCCTACAGATGATCAGTTGAGTTTAGCGGACATAGACACAATTAAAGTCATTGGAAAAGGAAATGGTGGAACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTAATTGAGATGAAAATTGAGGAGTCTTACCGCAGGCAGATTGCTAAAGAACTCAAAATCAATCAATCAGCACAGTGTCCCTATATTGTTGTCTGTTACCAATCTTTCTATGATAATGGGTCAATATATATCATTTTTGAGTACATGGATGGTGGATCTTTAGCTGATTTTCTGAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCATTTGTAAGCAGGTGCTGAAGGGGCTGATGTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCTAATTTATTAGTAAATCATAGAGGGGAAGTCAAGATTACCGACTTCGGTGTGAGTGCGATTATGGCAAGCACAACTGGAGAGGCTAATACTTTCGTTGGCACATATAACTATATGTCTCCAGAGAGAATTGTTGGAAATAGATATGGCAAAAAAAGTGACATTTGGAGCTTGGGGCTGATATTACTTGAATGTGCAACCGGCAAATTCCCTTATTCTCCACCCGAGAAAGATGGAGGATGGGGTAACTTTTATGATCTTATGGTAGCCATTGCTGAGGGCGAACCTCCTTCTGCTCCAGCCGACCAGTTTACTCCCAACTTCTGTTCCTTCATTTCTGCATGTTTGCAAACGGAGCCGAAGGATAGACTGTCAGCACGTGAACTTTTGGAACACCCTTTCGTCAACATGTATGGAGATACAGATATTGACCTGTCATCTTACTTCAATGATGCAGGGACTCCACTTGCAACGTTCTAA

Protein sequence

MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDSVSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVPSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQHKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYMDGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKITDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYSPPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHPFVNMYGDTDIDLSSYFNDAGTPLATF
Homology
BLAST of Cp4.1LG04g10980 vs. ExPASy Swiss-Prot
Match: Q9S7U9 (Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK2 PE=1 SV=2)

HSP 1 Score: 482.6 bits (1241), Expect = 4.7e-135
Identity = 225/325 (69.23%), Postives = 271/325 (83.38%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DGVRI+SQ E E   PIKP DDQLSL+D+D +KVIGKG+ G VQLVQ
Sbjct: 29  SGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQ 88

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWT QFFALKVI++ I+E+ R+ IA+ELKINQS+QCP +V  YQSFYDNG+I +I EYM
Sbjct: 89  HKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYM 148

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLK VK I + YL+AI +QVL+GL+YLHH+RHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 149 DGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKI 208

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS +M +T G ANTFVGTYNYMSPERIVGN+YG KSDIWSLGL++LECATGKFPY+
Sbjct: 209 TDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYA 268

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PP ++  W + ++LM AI +  PP+ P+  F+P   SFIS CLQ +P  R SA+EL+EHP
Sbjct: 269 PPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQKDPNSRSSAKELMEHP 328

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLAT 446
           F+N Y  + I+L+SYF DAG+PLAT
Sbjct: 329 FLNKYDYSGINLASYFTDAGSPLAT 353

BLAST of Cp4.1LG04g10980 vs. ExPASy Swiss-Prot
Match: Q94A06 (Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK1 PE=1 SV=2)

HSP 1 Score: 460.7 bits (1184), Expect = 1.9e-128
Identity = 221/327 (67.58%), Postives = 266/327 (81.35%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DG++ VS SE  APPPI+P D+QLSLAD++ IKVIGKG+ G VQLV+
Sbjct: 27  SGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVK 86

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HK T QFFALKVI++  EES  R I++EL+IN S+QCPY+V CYQSFY NG + II E+M
Sbjct: 87  HKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSFYHNGLVSIILEFM 146

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLAD LKKV K+ E  L+AICK+VL+GL Y+HHER IIHRDLKPSNLL+NHRGEVKI
Sbjct: 147 DGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKI 206

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS I+ ST+  AN+FVGTY YMSPERI G+ Y  KSDIWSLGL+LLECATGKFPY+
Sbjct: 207 TDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGLVLLECATGKFPYT 266

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPE   GW + Y+L+ AI E  PP AP++ F+P FCSFIS C+Q +P+DR SA+ELLEH 
Sbjct: 267 PPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDPRDRKSAKELLEHK 326

Query: 421 FVNMYGDTDIDLSSYFNDAGT---PLA 445
           FV M+ D+D +LS+YF DAG+   PLA
Sbjct: 327 FVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of Cp4.1LG04g10980 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.1e-115
Identity = 192/314 (61.15%), Postives = 253/314 (80.57%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPP--IKPTDDQLSLADIDTIKVIGKGNGGTVQL 180
           SGTFKDG+L +N+ G++++S+   + P    +K  D QLS+ D++ I+VIGKG+GG VQL
Sbjct: 28  SGTFKDGELRLNQRGLQLISEETADEPQSTNLKVEDVQLSMDDLEMIQVIGKGSGGIVQL 87

Query: 181 VQHKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFE 240
           V+HKW    +ALK I+M I+E+ R+QI +ELKINQ+ Q  +IV+C+QSFY NG IY++ E
Sbjct: 88  VRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHIVLCHQSFYHNGVIYLVLE 147

Query: 241 YMDGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEV 300
           YMD GSLAD +K+VK I EPYLA +CKQVL+GL+YLHHERH+IHRD+KPSNLLVN +GEV
Sbjct: 148 YMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHVIHRDIKPSNLLVNRKGEV 207

Query: 301 KITDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFP 360
           KITDFGVSA++AS+ G+ +TFVGTYNYM+PERI G+ Y  KSDIWSLGL++LECA G+FP
Sbjct: 208 KITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKSDIWSLGLVILECAIGRFP 267

Query: 361 YSPPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLE 420
           Y P E + GW +FY+L+ AI +  PPSAPADQF+P FC+FIS+C+Q +P +R+SA ELL 
Sbjct: 268 YIPSEGE-GWLSFYELLEAIVDQPPPSAPADQFSPEFCAFISSCIQKDPAERMSASELLN 327

Query: 421 HPFVNMYGDTDIDL 433
           HPF+  + D D+DL
Sbjct: 328 HPFIKKFEDKDLDL 340

BLAST of Cp4.1LG04g10980 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 5.4e-115
Identity = 191/315 (60.63%), Postives = 248/315 (78.73%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTF+DGD+++N++G+R+VSQ++   P      +  +SL+D++T+KV+GKG+GG VQLV+
Sbjct: 32  SGTFQDGDIMLNKEGIRVVSQTQTSTP----AANGNISLSDLETVKVLGKGSGGVVQLVR 91

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWT + +ALKVI M IEE+ R+QI +ELKIN ++QCPY+V+CY +FY+NG I I+ EYM
Sbjct: 92  HKWTNETYALKVIHMNIEETTRKQIVQELKINHASQCPYVVICYHAFYNNGLISIVLEYM 151

Query: 241 DGGSLADFLKKV--KKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEV 300
           DGGSLAD +K++  K I+EP L  +CKQVL GLMYLH  RHIIHRD+KPSNLLVNH+GEV
Sbjct: 152 DGGSLADIMKELEPKCIKEPNLGVVCKQVLLGLMYLHQTRHIIHRDIKPSNLLVNHKGEV 211

Query: 301 KITDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFP 360
           KI+DFGVSA++A++    +TFVGT  YMSPER++G  YG  SDIWSLGL LLECA GK+P
Sbjct: 212 KISDFGVSAVLANSMAVRDTFVGTCTYMSPERVLGGTYGFDSDIWSLGLTLLECALGKYP 271

Query: 361 YSPPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLE 420
           Y PP  + GW NFY+L+  I +  PP APADQF+P FCSFISACLQ +PK R +A ELL 
Sbjct: 272 YQPPGSEEGWMNFYELLQTIVDQPPPVAPADQFSPEFCSFISACLQKDPKCRPTAAELLN 331

Query: 421 HPFVNMYGDTDIDLS 434
           HPFVN Y + +  L+
Sbjct: 332 HPFVNKYDEEEYGLA 342

BLAST of Cp4.1LG04g10980 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-111
Identity = 186/313 (59.42%), Postives = 242/313 (77.32%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTF DGD L+N+ G+R+ S  +       K  D +++  D++T+KVIGKG+GG VQLV+
Sbjct: 29  SGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKELDFEITAEDLETVKVIGKGSGGVVQLVR 88

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQ-SAQCPYIVVCYQSFYDNGSIYIIFEY 240
           HKW  +FFA+KVI+M I+E  R+QI +ELKINQ S+QCP++VVCY SFY NG+  ++ EY
Sbjct: 89  HKWVGKFFAMKVIQMNIQEEIRKQIVQELKINQASSQCPHVVVCYHSFYHNGAFSLVLEY 148

Query: 241 MDGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVK 300
           MD GSLAD +++VK I EPYLA +CKQVL GL+YLH+ERH+IHRD+KPSNLLVNH+GEVK
Sbjct: 149 MDRGSLADVIRQVKTILEPYLAVVCKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVK 208

Query: 301 ITDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPY 360
           I+DFGVSA +AS+ G+ +TFVGTYNYMSPERI G+ Y   SDIWSLG+ +LECA G+FPY
Sbjct: 209 ISDFGVSASLASSMGQRDTFVGTYNYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPY 268

Query: 361 SPPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEH 420
              E      +FY+L+ AI E  PP+AP+DQF+P FCSF+SAC+Q +P  R S+ +LL H
Sbjct: 269 LESEDQQNPPSFYELLAAIVENPPPTAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSH 328

Query: 421 PFVNMYGDTDIDL 433
           PF+  + D DIDL
Sbjct: 329 PFIKKFEDKDIDL 341

BLAST of Cp4.1LG04g10980 vs. NCBI nr
Match: XP_023531041.1 (mitogen-activated protein kinase kinase 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023531042.1 mitogen-activated protein kinase kinase 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023531043.1 mitogen-activated protein kinase kinase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687 bits (1773), Expect = 2.06e-247
Identity = 355/446 (79.60%), Postives = 355/446 (79.60%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM
Sbjct: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           FVNMYGDTDIDLSSYFNDAGTPLATF
Sbjct: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 355

BLAST of Cp4.1LG04g10980 vs. NCBI nr
Match: KAG7021953.1 (Mitogen-activated protein kinase kinase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 682 bits (1759), Expect = 2.80e-245
Identity = 353/446 (79.15%), Postives = 353/446 (79.15%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYII EYM
Sbjct: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           FVNMY DTDIDLSSYFNDAGTPLATF
Sbjct: 421 FVNMYEDTDIDLSSYFNDAGTPLATF 355

BLAST of Cp4.1LG04g10980 vs. NCBI nr
Match: XP_022933979.1 (mitogen-activated protein kinase kinase 2-like [Cucurbita moschata] >XP_022933980.1 mitogen-activated protein kinase kinase 2-like [Cucurbita moschata])

HSP 1 Score: 678 bits (1749), Expect = 9.34e-244
Identity = 352/446 (78.92%), Postives = 352/446 (78.92%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYII EYM
Sbjct: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAI KQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAAISKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           FVNMY DTDIDLSSYFNDAGTPLATF
Sbjct: 421 FVNMYEDTDIDLSSYFNDAGTPLATF 355

BLAST of Cp4.1LG04g10980 vs. NCBI nr
Match: XP_022967709.1 (mitogen-activated protein kinase kinase 2-like [Cucurbita maxima] >XP_022967716.1 mitogen-activated protein kinase kinase 2-like [Cucurbita maxima])

HSP 1 Score: 670 bits (1729), Expect = 1.04e-240
Identity = 347/446 (77.80%), Postives = 350/446 (78.48%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNL+LKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 1   MRKGGLSNNLNLKLSLPQEDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNG IYII EYM
Sbjct: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGLIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERI+GNR+GKKSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIIGNRHGKKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGW NFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 361 PPEKDGGWDNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           FV MY DTDIDLSSYFNDAGTPLATF
Sbjct: 421 FVKMYEDTDIDLSSYFNDAGTPLATF 355

BLAST of Cp4.1LG04g10980 vs. NCBI nr
Match: KAG6588058.1 (Mitogen-activated protein kinase kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 639 bits (1648), Expect = 7.90e-228
Identity = 332/424 (78.30%), Postives = 332/424 (78.30%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 31  MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT---------------------------- 90

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 91  ------------------------------------------------------------ 150

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 151 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 210

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYII EYM
Sbjct: 211 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIILEYM 270

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 271 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 330

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS
Sbjct: 331 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 363

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 391 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 363

Query: 421 FVNM 424
           FVNM
Sbjct: 451 FVNM 363

BLAST of Cp4.1LG04g10980 vs. ExPASy TrEMBL
Match: A0A6J1F195 (mitogen-activated protein kinase kinase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111441227 PE=3 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 4.52e-244
Identity = 352/446 (78.92%), Postives = 352/446 (78.92%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYII EYM
Sbjct: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAI KQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAAISKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           FVNMY DTDIDLSSYFNDAGTPLATF
Sbjct: 421 FVNMYEDTDIDLSSYFNDAGTPLATF 355

BLAST of Cp4.1LG04g10980 vs. ExPASy TrEMBL
Match: A0A6J1HSX4 (mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111467153 PE=3 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 5.03e-241
Identity = 347/446 (77.80%), Postives = 350/446 (78.48%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGGLSNNL+LKLSLPQEDQSIAKFLTQSGT                            
Sbjct: 1   MRKGGLSNNLNLKLSLPQEDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNG IYII EYM
Sbjct: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGLIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMASTTGEANTFVGTYNYMSPERI+GNR+GKKSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIIGNRHGKKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPEKDGGW NFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP
Sbjct: 361 PPEKDGGWDNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           FV MY DTDIDLSSYFNDAGTPLATF
Sbjct: 421 FVKMYEDTDIDLSSYFNDAGTPLATF 355

BLAST of Cp4.1LG04g10980 vs. ExPASy TrEMBL
Match: A0A6J1H9J6 (mitogen-activated protein kinase kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC111461750 PE=3 SV=1)

HSP 1 Score: 625 bits (1612), Expect = 3.28e-223
Identity = 317/446 (71.08%), Postives = 340/446 (76.23%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGG SNNL+LKL+LPQ+DQSIAKFLTQSGT                            
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVS+SEVEAPPPIKPTDDQLSLADID IKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSKSEVEAPPPIKPTDDQLSLADIDIIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVI+MKIEES+R+QIA+ELKINQSAQCPYIVVCYQSFYDNGSIYII EYM
Sbjct: 181 HKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYIVVCYQSFYDNGSIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVK+++EPYLAA+CKQVLKGL YLHHERHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 241 DGGSLADFLKKVKQVQEPYLAALCKQVLKGLSYLHHERHIIHRDLKPSNLLINHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMAST GEANTFVGTYNYMSPERI+G +Y  KSDIWSLGLI LECATGKFPYS
Sbjct: 301 TDFGVSAIMASTYGEANTFVGTYNYMSPERIIGEKYDNKSDIWSLGLIFLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPE++GGW NFY+LM AI EGEPPSAPADQFTPNFCSFISAC+Q +P+DRLSARELLEHP
Sbjct: 361 PPEQEGGWVNFYELMEAIVEGEPPSAPADQFTPNFCSFISACVQKDPRDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           F+ MY DTDIDLSSYFNDAG+PLATF
Sbjct: 421 FIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cp4.1LG04g10980 vs. ExPASy TrEMBL
Match: A0A6J1JJS8 (mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485219 PE=3 SV=1)

HSP 1 Score: 624 bits (1609), Expect = 9.38e-223
Identity = 316/446 (70.85%), Postives = 340/446 (76.23%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGG SNNL+LKL+LPQ+DQSIAKFLTQSGT                            
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVS+SEVEAPPPIKPTDDQLSLADID IKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSKSEVEAPPPIKPTDDQLSLADIDIIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVI+MKIEES+R+QIA+ELKINQSAQCPYIVVCYQSFYDNGSIYII EYM
Sbjct: 181 HKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYIVVCYQSFYDNGSIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVK+++EPYLAA+CKQVLKGL YLHH+RHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 241 DGGSLADFLKKVKQVQEPYLAALCKQVLKGLSYLHHDRHIIHRDLKPSNLLINHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIMAST GEANTFVGTYNYMSPERI+G +Y  KSDIWSLGLI LECATGKFPYS
Sbjct: 301 TDFGVSAIMASTYGEANTFVGTYNYMSPERIIGEKYDNKSDIWSLGLIFLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPE++GGW NFY+LM AI EGEPPSAPADQFTPNFCSFISAC+Q +P+DRLSARELLEHP
Sbjct: 361 PPEQEGGWVNFYELMEAIVEGEPPSAPADQFTPNFCSFISACVQKDPRDRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           F+ MY DTDIDLSSYFNDAG+PLATF
Sbjct: 421 FIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cp4.1LG04g10980 vs. ExPASy TrEMBL
Match: A0A0A0LWW3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589750 PE=3 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 7.32e-220
Identity = 317/446 (71.08%), Postives = 335/446 (75.11%), Query Frame = 0

Query: 1   MRKGGLSNNLDLKLSLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSQSEVEADVFDS 60
           MRKGG SNNL+LKL+LP+EDQSIA FLTQSGT                            
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGT---------------------------- 60

Query: 61  VSVASSHRIITRSFDLSIDSTGVGAWKWKISTINEEGRLKQQSRPQTQSPSGRPIHSQVP 120
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
              FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADID IKVIGKGNGGTVQLVQ
Sbjct: 121 ---FKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDIIKVIGKGNGGTVQLVQ 180

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWTAQFFALKVI+MKIEES+R+QIA+ELKINQSAQCPY+VVCYQSFYDNGSIYII EYM
Sbjct: 181 HKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQSFYDNGSIYIILEYM 240

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLKKVKKIEEPYLAA+CKQVLKGL YLHHERHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 241 DGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLKPSNLLINHRGEVKI 300

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVSAIM +T  EANTFVGTYNYMSPERIVG  Y  KSDIWSLGLILLECATGKFPYS
Sbjct: 301 TDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLGLILLECATGKFPYS 355

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PP +DGGW NFY+LM AI EGEPPSAPADQFTP FCSFISAC+QT+PK+RLSARELLEHP
Sbjct: 361 PPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTDPKNRLSARELLEHP 355

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLATF 446
           F+ MY D DIDLSSYFNDAG+PLATF
Sbjct: 421 FIKMYEDKDIDLSSYFNDAGSPLATF 355

BLAST of Cp4.1LG04g10980 vs. TAIR 10
Match: AT4G29810.1 (MAP kinase kinase 2 )

HSP 1 Score: 482.6 bits (1241), Expect = 3.3e-136
Identity = 225/325 (69.23%), Postives = 271/325 (83.38%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DGVRI+SQ E E   PIKP DDQLSL+D+D +KVIGKG+ G VQLVQ
Sbjct: 29  SGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQ 88

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWT QFFALKVI++ I+E+ R+ IA+ELKINQS+QCP +V  YQSFYDNG+I +I EYM
Sbjct: 89  HKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYM 148

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLK VK I + YL+AI +QVL+GL+YLHH+RHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 149 DGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKI 208

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS +M +T G ANTFVGTYNYMSPERIVGN+YG KSDIWSLGL++LECATGKFPY+
Sbjct: 209 TDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYA 268

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PP ++  W + ++LM AI +  PP+ P+  F+P   SFIS CLQ +P  R SA+EL+EHP
Sbjct: 269 PPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQKDPNSRSSAKELMEHP 328

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLAT 446
           F+N Y  + I+L+SYF DAG+PLAT
Sbjct: 329 FLNKYDYSGINLASYFTDAGSPLAT 353

BLAST of Cp4.1LG04g10980 vs. TAIR 10
Match: AT4G29810.2 (MAP kinase kinase 2 )

HSP 1 Score: 482.6 bits (1241), Expect = 3.3e-136
Identity = 225/325 (69.23%), Postives = 271/325 (83.38%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DGVRI+SQ E E   PIKP DDQLSL+D+D +KVIGKG+ G VQLVQ
Sbjct: 38  SGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQ 97

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWT QFFALKVI++ I+E+ R+ IA+ELKINQS+QCP +V  YQSFYDNG+I +I EYM
Sbjct: 98  HKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYM 157

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLK VK I + YL+AI +QVL+GL+YLHH+RHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 158 DGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKI 217

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS +M +T G ANTFVGTYNYMSPERIVGN+YG KSDIWSLGL++LECATGKFPY+
Sbjct: 218 TDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYA 277

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PP ++  W + ++LM AI +  PP+ P+  F+P   SFIS CLQ +P  R SA+EL+EHP
Sbjct: 278 PPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQKDPNSRSSAKELMEHP 337

Query: 421 FVNMYGDTDIDLSSYFNDAGTPLAT 446
           F+N Y  + I+L+SYF DAG+PLAT
Sbjct: 338 FLNKYDYSGINLASYFTDAGSPLAT 362

BLAST of Cp4.1LG04g10980 vs. TAIR 10
Match: AT4G26070.2 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 460.7 bits (1184), Expect = 1.3e-129
Identity = 221/327 (67.58%), Postives = 266/327 (81.35%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DG++ VS SE  APPPI+P D+QLSLAD++ IKVIGKG+ G VQLV+
Sbjct: 27  SGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVK 86

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HK T QFFALKVI++  EES  R I++EL+IN S+QCPY+V CYQSFY NG + II E+M
Sbjct: 87  HKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSFYHNGLVSIILEFM 146

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLAD LKKV K+ E  L+AICK+VL+GL Y+HHER IIHRDLKPSNLL+NHRGEVKI
Sbjct: 147 DGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKI 206

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS I+ ST+  AN+FVGTY YMSPERI G+ Y  KSDIWSLGL+LLECATGKFPY+
Sbjct: 207 TDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGLVLLECATGKFPYT 266

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPE   GW + Y+L+ AI E  PP AP++ F+P FCSFIS C+Q +P+DR SA+ELLEH 
Sbjct: 267 PPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDPRDRKSAKELLEHK 326

Query: 421 FVNMYGDTDIDLSSYFNDAGT---PLA 445
           FV M+ D+D +LS+YF DAG+   PLA
Sbjct: 327 FVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of Cp4.1LG04g10980 vs. TAIR 10
Match: AT4G26070.3 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 460.7 bits (1184), Expect = 1.3e-129
Identity = 221/327 (67.58%), Postives = 266/327 (81.35%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DG++ VS SE  APPPI+P D+QLSLAD++ IKVIGKG+ G VQLV+
Sbjct: 27  SGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVK 86

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HK T QFFALKVI++  EES  R I++EL+IN S+QCPY+V CYQSFY NG + II E+M
Sbjct: 87  HKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSFYHNGLVSIILEFM 146

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLAD LKKV K+ E  L+AICK+VL+GL Y+HHER IIHRDLKPSNLL+NHRGEVKI
Sbjct: 147 DGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKI 206

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS I+ ST+  AN+FVGTY YMSPERI G+ Y  KSDIWSLGL+LLECATGKFPY+
Sbjct: 207 TDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGLVLLECATGKFPYT 266

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELLEHP 420
           PPE   GW + Y+L+ AI E  PP AP++ F+P FCSFIS C+Q +P+DR SA+ELLEH 
Sbjct: 267 PPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDPRDRKSAKELLEHK 326

Query: 421 FVNMYGDTDIDLSSYFNDAGT---PLA 445
           FV M+ D+D +LS+YF DAG+   PLA
Sbjct: 327 FVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of Cp4.1LG04g10980 vs. TAIR 10
Match: AT4G29810.3 (MAP kinase kinase 2 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.2e-124
Identity = 207/297 (69.70%), Postives = 248/297 (83.50%), Query Frame = 0

Query: 121 SGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDTIKVIGKGNGGTVQLVQ 180
           SGTFKDGDL VN+DGVRI+SQ E E   PIKP DDQLSL+D+D +KVIGKG+ G VQLVQ
Sbjct: 38  SGTFKDGDLRVNKDGVRIISQLEPEVLSPIKPADDQLSLSDLDMVKVIGKGSSGVVQLVQ 97

Query: 181 HKWTAQFFALKVIEMKIEESYRRQIAKELKINQSAQCPYIVVCYQSFYDNGSIYIIFEYM 240
           HKWT QFFALKVI++ I+E+ R+ IA+ELKINQS+QCP +V  YQSFYDNG+I +I EYM
Sbjct: 98  HKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQCPNLVTSYQSFYDNGAISLILEYM 157

Query: 241 DGGSLADFLKKVKKIEEPYLAAICKQVLKGLMYLHHERHIIHRDLKPSNLLVNHRGEVKI 300
           DGGSLADFLK VK I + YL+AI +QVL+GL+YLHH+RHIIHRDLKPSNLL+NHRGEVKI
Sbjct: 158 DGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRDLKPSNLLINHRGEVKI 217

Query: 301 TDFGVSAIMASTTGEANTFVGTYNYMSPERIVGNRYGKKSDIWSLGLILLECATGKFPYS 360
           TDFGVS +M +T G ANTFVGTYNYMSPERIVGN+YG KSDIWSLGL++LECATGKFPY+
Sbjct: 218 TDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWSLGLVVLECATGKFPYA 277

Query: 361 PPEKDGGWGNFYDLMVAIAEGEPPSAPADQFTPNFCSFISACLQTEPKDRLSARELL 418
           PP ++  W + ++LM AI +  PP+ P+  F+P   SFIS CLQ +P  R SA+EL+
Sbjct: 278 PPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQKDPNSRSSAKELM 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7U94.7e-13569.23Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q94A061.9e-12867.58Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q5QN754.1e-11561.15Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399... [more]
A9RWC95.4e-11560.63Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK... [more]
Q9FJV01.2e-11159.42Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
XP_023531041.12.06e-24779.60mitogen-activated protein kinase kinase 2-like isoform X1 [Cucurbita pepo subsp.... [more]
KAG7021953.12.80e-24579.15Mitogen-activated protein kinase kinase 2, partial [Cucurbita argyrosperma subsp... [more]
XP_022933979.19.34e-24478.92mitogen-activated protein kinase kinase 2-like [Cucurbita moschata] >XP_02293398... [more]
XP_022967709.11.04e-24077.80mitogen-activated protein kinase kinase 2-like [Cucurbita maxima] >XP_022967716.... [more]
KAG6588058.17.90e-22878.30Mitogen-activated protein kinase kinase 2, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1F1954.52e-24478.92mitogen-activated protein kinase kinase 2-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HSX45.03e-24177.80mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1H9J63.28e-22371.08mitogen-activated protein kinase kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JJS89.38e-22370.85mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A0A0LWW37.32e-22071.08Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589... [more]
Match NameE-valueIdentityDescription
AT4G29810.13.3e-13669.23MAP kinase kinase 2 [more]
AT4G29810.23.3e-13669.23MAP kinase kinase 2 [more]
AT4G26070.21.3e-12967.58MAP kinase/ ERK kinase 1 [more]
AT4G26070.31.3e-12967.58MAP kinase/ ERK kinase 1 [more]
AT4G29810.32.2e-12469.70MAP kinase kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 162..422
e-value: 1.2E-89
score: 313.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 164..422
e-value: 4.1E-71
score: 239.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 162..422
score: 48.371506
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 242..441
e-value: 1.2E-62
score: 213.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 138..241
e-value: 3.0E-28
score: 99.9
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 163..362
e-value: 7.6E-29
score: 98.2
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 104..399
e-value: 5.5E-15
score: 53.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..125
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..118
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 2..56
NoneNo IPR availablePANTHERPTHR24361:SF759MAP KINASE KINASEcoord: 2..56
NoneNo IPR availablePANTHERPTHR24361:SF759MAP KINASE KINASEcoord: 120..445
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 120..445
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 160..425
e-value: 7.65201E-152
score: 429.705
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 168..191
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 280..292
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 147..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g10980.1Cp4.1LG04g10980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity