Cp4.1LG04g08650 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g08650 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.0e-28
Identity = 132/464 (28.45%), Postives = 188/464 (40.52%), Query Frame = 0

Query: 81  KFLRLRTLSFDSSIFSTFQVLPKSIGKLKHLRYLNICLYELQFLPNSITKLYNLETLILD 140
           KF+ LR L+      STF  LP SIG L HLRYLN+    ++ LP  + KL NL+TL L 
Sbjct: 524 KFISLRVLNLGD---STFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQ 583

Query: 141 WCEHLQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDI 200
           +C  L  LP++      LR L L G+  +  +P  I SL  L+TL               
Sbjct: 584 YCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTL--------------- 643

Query: 201 KKCVNLKHLDLRGCNALTHLPKG--LGELTNLQTMNLCWLRDLSELNRLSKLRGSLTIKG 260
                           +    KG  LGEL NL                   L GS+ I  
Sbjct: 644 -------------GQFVVGRKKGYQLGELGNL------------------NLYGSIKISH 703

Query: 261 LEVCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRK 320
           LE     D +    NL  K     L   WN +   H   + ++E    L  L+PHSN+  
Sbjct: 704 LE-RVKNDKDAKEANLSAKGNLHSLSMSWN-NFGPH---IYESEEVKVLEALKPHSNLTS 763

Query: 321 IDICRYPEVMLCDWLSSNTLLHLV------------LPQFDQFPCLKCL----GLKSLPC 380
           + I  +  + L +W++ + L ++V            LP F   PCL+ L    G   +  
Sbjct: 764 LKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEY 823

Query: 381 IEYIDNDGYRS--SSIFFPSLEKLSIIDMHNLKGWWKVSLQLLNVIIEKT-------ANP 440
           +E +D D +    + I FPSL KL I D  +LKG  K   +    ++E+           
Sbjct: 824 VEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKKEGEEQFPVLEEMIIHECPFLTL 883

Query: 441 SSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNL----------------- 500
           SS    L+ + +         P+E+F +  NL +L I  C NL                 
Sbjct: 884 SSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLK 931

BLAST of Cp4.1LG04g08650 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 1.5e-27
Identity = 122/441 (27.66%), Postives = 204/441 (46.26%), Query Frame = 0

Query: 123 FLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNL 182
           + P+ + K  +L  L L    +L +LP    + + LRYL L+GN  I+ LP  +  LQNL
Sbjct: 516 YSPSLLQKFVSLRVLNLR-NSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNL 575

Query: 183 ETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWL---- 242
           +TL L  C  L  LPK   K  +L++L L GC +LT  P  +G LT L++++ C++    
Sbjct: 576 QTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGC-SLTSTPPRIGLLTCLKSLS-CFVIGKR 635

Query: 243 --RDLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMN 302
               L EL  L+ L GS++I  L+    +D +    NL  K     L   W+        
Sbjct: 636 KGHQLGELKNLN-LYGSISITKLD-RVKKDTDAKEANLSAKANLHSLCLSWD-------- 695

Query: 303 IVLDNECKGD---LNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHLV----------- 362
             LD + + D   L  L+PHSN++ ++I  +  + L DW++ + L ++V           
Sbjct: 696 --LDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCS 755

Query: 363 -LPQFDQFPCLKCLGLKSLPC-IEYIDNDGYRSSSIFFPSLEKLSIIDMHNLKGWWKVSL 422
            LP F + PCL+ L L +    +EY++++ +      FPSL KL I D  NLKG  K+  
Sbjct: 756 CLPPFGELPCLESLELHTGSADVEYVEDNVHPGR---FPSLRKLVIWDFSNLKGLLKMEG 815

Query: 423 QLLNVIIEKTA---NPSSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNLQ 482
           +    ++E+      P      LS V   K+   +        +   L  L I D  N++
Sbjct: 816 EKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISD--NVE 875

Query: 483 LSSALVQHPTNDVLWKEFQSLRVLFLRAIPKLEYLPNGMQHI----AFRFKMSPRIKDII 535
            +S        + ++K   +L+ L +     L+ LP  +  +    + +F+    ++ + 
Sbjct: 876 ATS------LPEEMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLP 929

BLAST of Cp4.1LG04g08650 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 4.4e-27
Identity = 124/445 (27.87%), Postives = 197/445 (44.27%), Query Frame = 0

Query: 123 FLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNL 182
           + P+   +  +L  L L   E  ++LP    + + LRYL L+GN +I  LP  +  LQNL
Sbjct: 526 YSPSLFKRFVSLRVLNLSNSE-FEQLPSSVGDLVHLRYLDLSGN-KICSLPKRLCKLQNL 585

Query: 183 ETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWLRD-- 242
           +TL L +C  L  LPK   K  +L++L L  C  LT +P  +G LT L+T+    + +  
Sbjct: 586 QTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC-PLTSMPPRIGLLTCLKTLGYFVVGERK 645

Query: 243 ---LSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQELKFVW---NYSKDEH 302
              L EL  L+ LRG+++I  LE     D+E    NL  K     L   W   N  + E 
Sbjct: 646 GYQLGELRNLN-LRGAISITHLE-RVKNDMEAKEANLSAKANLHSLSMSWDRPNRYESEE 705

Query: 303 MNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHLV------------ 362
           + +         L  L+PH N++ ++I  +    L DW++ + L ++V            
Sbjct: 706 VKV---------LEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSC 765

Query: 363 LPQFDQFPCLKCLGLKSLPC-IEYIDNDGYRSSSIFFPSLEKLSIIDMHNLKGWWKV--- 422
           LP F + PCL+ L L+     +EY+++ G+ +    FPSL KL I    NLKG  ++   
Sbjct: 766 LPPFGELPCLESLELQDGSVEVEYVEDSGFLTRR-RFPSLRKLHIGGFCNLKGLQRMKGA 825

Query: 423 -----------------------SLQLLNVIIEKTANPSSTPSKLSHVYLSKI---DDLE 482
                                  S++ L +  E  A   S+ S LS +   KI     + 
Sbjct: 826 EQFPVLEEMKISDCPMFVFPTLSSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVT 885

Query: 483 FLPKELFYHFTNLLFLVIE------------------DCKNLQLSSALVQHPTNDVLWKE 500
            L +E+F +  NL++L +                    C +++   AL   P   +  + 
Sbjct: 886 SLLEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGL--EG 945

BLAST of Cp4.1LG04g08650 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 7.5e-27
Identity = 130/457 (28.45%), Postives = 207/457 (45.30%), Query Frame = 0

Query: 109 KHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGNTE 168
           KH   +        + P+ + K  +L  L L + + L++LP    + + LRYL L+ N  
Sbjct: 504 KHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSYSK-LEQLPSSIGDLLHLRYLDLSCN-N 563

Query: 169 IKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELT 228
            + LP+ +  LQNL+TL + +C  L  LPK   K  +L+HL + GC  LT  P  +G LT
Sbjct: 564 FRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC-PLTSTPPRIGLLT 623

Query: 229 NLQTMNLCWL-----RDLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQEL 288
            L+T+    +       L EL  L+ L GS++I  LE    ++  ++  NL  K   Q L
Sbjct: 624 CLKTLGFFIVGSKKGYQLGELKNLN-LCGSISITHLE--RVKNDTDAEANLSAKANLQSL 683

Query: 289 KFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHLV- 348
              W+   ++  N     E K  L  L+PH N++ ++I  +       W++ + L  ++ 
Sbjct: 684 SMSWD---NDGPNRYESKEVK-VLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVIS 743

Query: 349 -----------LPQFDQFPCLKCLGLKSLPC-IEYIDNDGYR---SSSIFFPSLEKLSII 408
                      LP F + PCL+ L L++    +EY++ D      S+   FPSL+KL I 
Sbjct: 744 VRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIW 803

Query: 409 DMHNLKGWWKVSLQLLNVIIEKTA--------NPSSTPSKLSHVY-------LSKIDDLE 468
              +LKG  K   +    ++E+ A         P+ +  K   V+       LS I +L 
Sbjct: 804 FFRSLKGLMKEEGEEKFPMLEEMAILYCPLFVFPTLSSVKKLEVHGNTNTRGLSSISNLS 863

Query: 469 --------------FLPKELFYHFTNLLFLVIEDCKNLQ-LSSAL--------VQHPTND 500
                          LP+E+F   TNL FL   D KNL+ L ++L        +Q  + D
Sbjct: 864 TLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIESCD 923

BLAST of Cp4.1LG04g08650 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.8e-25
Identity = 137/523 (26.20%), Postives = 208/523 (39.77%), Query Frame = 0

Query: 82   FLRLRTLSFDSSIFSTFQVLPKSIGKLKHLRYLNICLYELQFLPNSITKLYNLETLILDW 141
            FL L   +   S      V  K +  L  LR L++  Y++  LP                
Sbjct: 551  FLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLP---------------- 610

Query: 142  CEHLQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIK 201
                   P   KN    R+L L+  TE++ LP S+  + NL+TL+L  CS L+ELP DI 
Sbjct: 611  -------PDFFKNISHARFLDLS-RTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDIS 670

Query: 202  KCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWL-----RDLSELNRLSKLRGSLTI 261
              +NL++LDL G   L  +P+  G L +LQT+   ++       +SEL  L  L G L I
Sbjct: 671  NLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKI 730

Query: 262  KGLE-VCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKGDLNC---LQP 321
              L+ V    D   +  NL  K   +E+ FVW        N    +  + +      L+P
Sbjct: 731  VELQRVVDVADAAEA--NLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRP 790

Query: 322  HSNVRKIDICRYPEVMLCDWLSSNTLLHLV------------LPQFDQFPCLKCLGLKSL 381
            H ++ K+ I RY      DWLS  +   +V            LP   Q PCLK L +  +
Sbjct: 791  HRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGM 850

Query: 382  PCIEYIDNDGYRSSSIF-------FPSLEKLSIIDMHNLKGWWKV---------SLQLLN 441
              ++ I    Y S           F SLE L   ++ + + W  V         SL+ L 
Sbjct: 851  VGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLF 910

Query: 442  VI--IEKTAN-PSSTPSKLS-HVYLSKIDDLEFLPKELFY-------------------- 501
            ++   E T   P+  PS +S H+Y  K   L+F P    Y                    
Sbjct: 911  ILRCPELTGTLPTFLPSLISLHIY--KCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKF 970

Query: 502  ---HFTNLLFLVIEDCKNLQLSSALVQHPTNDVLWKEFQSLRVLFLRAIPKLEYLPNGMQ 541
               HF NL  L ++ C +L       +H       +  +      L+ +PKL  LP  +Q
Sbjct: 971  PLNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLNALPQNLQ 1030

BLAST of Cp4.1LG04g08650 vs. NCBI nr
Match: XP_022931674.1 (putative disease resistance protein RGA1 [Cucurbita moschata] >XP_022931675.1 putative disease resistance protein RGA1 [Cucurbita moschata])

HSP 1 Score: 830 bits (2143), Expect = 5.15e-289
Identity = 434/519 (83.62%), Postives = 439/519 (84.59%), Query Frame = 0

Query: 47   LENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 106
            LENLKLLYEAKNL+TLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG
Sbjct: 544  LENLKLLYEAKNLRTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 603

Query: 107  KLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGN 166
            KLKHLRYLNICLYELQFLPNSITKLYNLETLILD CEHLQELPRDAKNWMKLRYLSLTGN
Sbjct: 604  KLKHLRYLNICLYELQFLPNSITKLYNLETLILDRCEHLQELPRDAKNWMKLRYLSLTGN 663

Query: 167  TEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE 226
            TEIKFLPDSIASL NLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE
Sbjct: 664  TEIKFLPDSIASLLNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE 723

Query: 227  LTNLQTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQ 286
            LTNLQTMNL  L      DLSELNRLSKLRGSLTIKGLEVCTTEDL+NSYFNLRLKLGFQ
Sbjct: 724  LTNLQTMNLFILNKDVGCDLSELNRLSKLRGSLTIKGLEVCTTEDLKNSYFNLRLKLGFQ 783

Query: 287  ELKFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHL 346
            ELKFVWNYSKDEHMNIVLDNECKG LNCLQPHSNV KIDIC YPEVMLCDWLSSNTLLHL
Sbjct: 784  ELKFVWNYSKDEHMNIVLDNECKGVLNCLQPHSNVGKIDICGYPEVMLCDWLSSNTLLHL 843

Query: 347  V------------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM 406
            V            LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM
Sbjct: 844  VSIKLSDCFQLQVLPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM 903

Query: 407  HNLKGWWK------------------------------------------------VSLQ 466
            HNLKGWWK                                                VSLQ
Sbjct: 904  HNLKGWWKGETSSEGSPNNASFPITMSCLCELKIDDCPQLASIPWHAPLKLLEICAVSLQ 963

Query: 467  LLNVIIEKTANPSSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNLQLSSA 500
            LLNVIIEKTANPSS   KLSHVYLSKIDD+EFLP+E FYHFTNLLFLVIEDCKNLQLSSA
Sbjct: 964  LLNVIIEKTANPSS---KLSHVYLSKIDDIEFLPRE-FYHFTNLLFLVIEDCKNLQLSSA 1023

BLAST of Cp4.1LG04g08650 vs. NCBI nr
Match: XP_023528691.1 (putative disease resistance protein RGA1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630 bits (1626), Expect = 5.09e-214
Identity = 333/524 (63.55%), Postives = 382/524 (72.90%), Query Frame = 0

Query: 47  LENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 106
           L++L+ L  AKNL+TLIIDAP  + Q VLN IP+ FLRLRTLS D S   T + L KS+G
Sbjct: 313 LKSLQSLLAAKNLRTLIIDAPGLEGQNVLNPIPSMFLRLRTLSLDLSCCGTVKALLKSVG 372

Query: 107 KLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGN 166
           KLKHLRYL IC Y+  FLPNSITKLYNLETLILDWC+ LQELP+DAKNWMKLRYLSLT N
Sbjct: 373 KLKHLRYLKICQYKHPFLPNSITKLYNLETLILDWCDCLQELPKDAKNWMKLRYLSLTRN 432

Query: 167 TEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE 226
            +IKFLPDSIA L NLETLILQ CS LRELPKDIKKCVNLKHLDL GCNALTHLPKG+GE
Sbjct: 433 WKIKFLPDSIAELHNLETLILQHCSRLRELPKDIKKCVNLKHLDLHGCNALTHLPKGIGE 492

Query: 227 LTNLQTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQ 286
           LT LQTMNL  L+     DLSELNRL+ L+GSLT+KGLE CTT+DLE S  NL LKLG Q
Sbjct: 493 LTRLQTMNLFVLKKDIGCDLSELNRLNNLKGSLTVKGLEACTTDDLEKSSLNLGLKLGVQ 552

Query: 287 ELKFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHL 346
           +LK VW YS+D+HMN VLDNECK  L CLQPHSNVR+I IC Y EV LCDWLSSNTL +L
Sbjct: 553 KLKLVWTYSEDQHMNNVLDNECKEVLKCLQPHSNVRRIHICGYREVRLCDWLSSNTLFNL 612

Query: 347 V------------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM 406
           V            LPQF+QFP LK LGLKSL  I+YIDN+ Y SSS+FFPSLEKLS++D+
Sbjct: 613 VSIKLSDCFKLQALPQFNQFPFLKHLGLKSLSNIDYIDNNEYHSSSMFFPSLEKLSMVDL 672

Query: 407 HNLKGWWK------------------------------------------------VSLQ 466
           HNLKGWWK                                                +S Q
Sbjct: 673 HNLKGWWKSENSSEASPNIASFPITMSCLCELEIDGCPHLASIPWQAPLKSLHLSAISSQ 732

Query: 467 LLNVIIEKTANP-----SSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNL 500
           LLNV+IE+T N      SST +K+S+ +LSKIDDLEFLP+ELFYHFTNL  L IEDC++L
Sbjct: 733 LLNVVIERTVNYCVEECSSTQTKVSYFHLSKIDDLEFLPEELFYHFTNLELLQIEDCQSL 792

BLAST of Cp4.1LG04g08650 vs. NCBI nr
Match: XP_023539563.1 (uncharacterized protein LOC111800203 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642 bits (1655), Expect = 6.20e-205
Identity = 340/521 (65.26%), Postives = 383/521 (73.51%), Query Frame = 0

Query: 45   EVLENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKS 104
            E LE+LKLL++AKNL+TLIIDA  +D QKV+NLI T+FL LRTL+ D    +T +VLP+S
Sbjct: 537  EKLESLKLLFKAKNLRTLIIDAVKEDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRS 596

Query: 105  IGKLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLT 164
            IGKLKHLRYL I   +L+FLPNSITKLYNLE LILD C+ LQELPRDAKNWMKLRYLSL 
Sbjct: 597  IGKLKHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLA 656

Query: 165  GNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGL 224
             N  IKFLP S+ +L NLETLILQDCS LRELPKDIKKCVNLKHLDL GCNALTH+ KGL
Sbjct: 657  RNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGL 716

Query: 225  GELTNLQTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLG 284
            GELT+LQTMNL  L+     DLSELNRL+KL+G LTIKGLEVCTT+DLE S FN+R KLG
Sbjct: 717  GELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLG 776

Query: 285  FQELKFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLL 344
             QEL  VWNYSKDEHMN ++DNEC G L+CLQPHS V+KIDIC+YP V LCDWLSSNTL+
Sbjct: 777  VQELNLVWNYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLV 836

Query: 345  HLV------------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSII 404
            HLV            LPQFDQFP LK LGLKSLP IEY DN+ Y SSS+FFPSLEKLSI+
Sbjct: 837  HLVSIKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIV 896

Query: 405  DMHNLKGWWK------------------------------------------------VS 464
            +M NLKGWWK                                                +S
Sbjct: 897  EMLNLKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAIS 956

Query: 465  LQLLNVIIEKTANPSSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNLQLS 500
             QLLNVI E+TAN        + V   KIDDLEFLP+  FYHFTNL  L IEDCKNL++S
Sbjct: 957  SQLLNVIFERTANHCVEECSSTFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMS 1016

BLAST of Cp4.1LG04g08650 vs. NCBI nr
Match: XP_023539490.1 (putative disease resistance protein RGA3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592 bits (1525), Expect = 2.36e-200
Identity = 320/520 (61.54%), Postives = 367/520 (70.58%), Query Frame = 0

Query: 47  LENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 106
           LE+LKLLYEAK L+TLIID+P +D +KV+ LI TKFLRLRTL+ + +I  T Q LPKSIG
Sbjct: 203 LESLKLLYEAKKLRTLIIDSPIRDFEKVVKLIFTKFLRLRTLNLNLAIEFTSQALPKSIG 262

Query: 107 KLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGN 166
           KLKHLRYL IC   L FLPNS+T+LYNLETLILD C  LQ+LPRDAKNW  LRYLSL  N
Sbjct: 263 KLKHLRYLKICQIGLCFLPNSMTELYNLETLILDGCMFLQKLPRDAKNWRNLRYLSLAEN 322

Query: 167 TEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE 226
             I+FLPDSI   +NLETLIL+ C LL+ELPKDIKKCV LKHLDL GC +LTH PKGLGE
Sbjct: 323 FRIEFLPDSIDEWKNLETLILRKCLLLKELPKDIKKCVKLKHLDLCGCESLTHSPKGLGE 382

Query: 227 LTNLQTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQ 286
           LT LQTMNL  L      DLSELNRLSKLRGSLTI GLE CTT+DLE   FNL+ KLG +
Sbjct: 383 LTRLQTMNLFVLNKDVGCDLSELNRLSKLRGSLTINGLEFCTTDDLEKHSFNLQQKLGVR 442

Query: 287 ELKFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHL 346
           +LK VWNYSKDE  NI  +++CKG L CLQP+SN + I IC Y EV LCDWL SN L HL
Sbjct: 443 KLKLVWNYSKDEPKNISANDDCKGVLECLQPNSNAQNIYICGYQEVKLCDWLCSNVLTHL 502

Query: 347 V------------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM 406
           V            LPQFDQFP LK LGL+SLPCIEY+DN+ Y SSS+F PSLEKL ++DM
Sbjct: 503 VSIKLSDCFKLEALPQFDQFPFLKHLGLQSLPCIEYVDNNEYPSSSMFLPSLEKLIMVDM 562

Query: 407 HNLKGWWK------------------------------------------------VSLQ 466
            NLKGWWK                                                V+LQ
Sbjct: 563 PNLKGWWKGETSSESSPNSASFPTTAPFLCQLKIDSCPKLASISWHAPLRKLQISDVTLQ 622

Query: 467 LLNVIIEKTAN-PSSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNLQLSS 500
           LLN +IE TAN  SST SK+S + L+++ DLEFLP+ELFY+FTNL  L I DCK+LQ+SS
Sbjct: 623 LLNTVIEMTANHSSSTQSKVSSLELNRMKDLEFLPRELFYNFTNLELLTITDCKSLQISS 682

BLAST of Cp4.1LG04g08650 vs. NCBI nr
Match: XP_022940308.1 (putative disease resistance protein RGA4 [Cucurbita moschata])

HSP 1 Score: 575 bits (1481), Expect = 6.98e-188
Identity = 335/623 (53.77%), Postives = 376/623 (60.35%), Query Frame = 0

Query: 47   LENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 106
            LE+LKLL++AKNL+TLIIDA  +D QKV+NLI T+FL LRTL  +    +T +VLP SIG
Sbjct: 592  LESLKLLFKAKNLKTLIIDAVNEDCQKVVNLISTQFLCLRTLKVEFCYTNTSKVLPSSIG 651

Query: 107  KLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGN 166
            KLKHLRYL IC  +L+FLPNSITKLYNLE LILDWCE LQELPRDAKNWMKLRYLSL  N
Sbjct: 652  KLKHLRYLRICQRKLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLRYLSLDRN 711

Query: 167  TEIKFLPDSIASLQNLETLIL--------------------------------------- 226
              IKFLPDSIA+L NLETLIL                                       
Sbjct: 712  VNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKFLPDSIGA 771

Query: 227  ---------------------------------------------------------QDC 286
                                                                     QDC
Sbjct: 772  LHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLETLILQDC 831

Query: 287  SLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWLR-----DLSELN 346
            S LRELPKDIKKCVNLKHL+L GC  LTH+PKGLGELT+LQTMNL  L+     DLSELN
Sbjct: 832  SELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIGCDLSELN 891

Query: 347  RLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKG 406
            RL KL GSLTI+GLEVCT + LE S   L LKLG QEL+ VWNYS+D+ MNIVLDNECKG
Sbjct: 892  RLDKLEGSLTIEGLEVCTFDVLEKS---LPLKLGVQELELVWNYSEDQDMNIVLDNECKG 951

Query: 407  DLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHLV------------LPQFDQFPCLK 466
                LQPHSN+R IDIC YPEV LCDWLSSNT +HLV            LPQFDQFP LK
Sbjct: 952  GFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFDQFPFLK 1011

Query: 467  CLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDMHNLKGWWK---------------- 500
             L L+SLP IEYIDN+ Y SSS+FFPSLE+LSI++M NLKGWWK                
Sbjct: 1012 YLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESSPNNASFPI 1071

BLAST of Cp4.1LG04g08650 vs. ExPASy TrEMBL
Match: A0A6J1EUW9 (putative disease resistance protein RGA1 OS=Cucurbita moschata OX=3662 GN=LOC111437812 PE=4 SV=1)

HSP 1 Score: 830 bits (2143), Expect = 2.49e-289
Identity = 434/519 (83.62%), Postives = 439/519 (84.59%), Query Frame = 0

Query: 47   LENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 106
            LENLKLLYEAKNL+TLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG
Sbjct: 544  LENLKLLYEAKNLRTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 603

Query: 107  KLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGN 166
            KLKHLRYLNICLYELQFLPNSITKLYNLETLILD CEHLQELPRDAKNWMKLRYLSLTGN
Sbjct: 604  KLKHLRYLNICLYELQFLPNSITKLYNLETLILDRCEHLQELPRDAKNWMKLRYLSLTGN 663

Query: 167  TEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE 226
            TEIKFLPDSIASL NLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE
Sbjct: 664  TEIKFLPDSIASLLNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGE 723

Query: 227  LTNLQTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQ 286
            LTNLQTMNL  L      DLSELNRLSKLRGSLTIKGLEVCTTEDL+NSYFNLRLKLGFQ
Sbjct: 724  LTNLQTMNLFILNKDVGCDLSELNRLSKLRGSLTIKGLEVCTTEDLKNSYFNLRLKLGFQ 783

Query: 287  ELKFVWNYSKDEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHL 346
            ELKFVWNYSKDEHMNIVLDNECKG LNCLQPHSNV KIDIC YPEVMLCDWLSSNTLLHL
Sbjct: 784  ELKFVWNYSKDEHMNIVLDNECKGVLNCLQPHSNVGKIDICGYPEVMLCDWLSSNTLLHL 843

Query: 347  V------------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM 406
            V            LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM
Sbjct: 844  VSIKLSDCFQLQVLPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDM 903

Query: 407  HNLKGWWK------------------------------------------------VSLQ 466
            HNLKGWWK                                                VSLQ
Sbjct: 904  HNLKGWWKGETSSEGSPNNASFPITMSCLCELKIDDCPQLASIPWHAPLKLLEICAVSLQ 963

Query: 467  LLNVIIEKTANPSSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNLQLSSA 500
            LLNVIIEKTANPSS   KLSHVYLSKIDD+EFLP+E FYHFTNLLFLVIEDCKNLQLSSA
Sbjct: 964  LLNVIIEKTANPSS---KLSHVYLSKIDDIEFLPRE-FYHFTNLLFLVIEDCKNLQLSSA 1023

BLAST of Cp4.1LG04g08650 vs. ExPASy TrEMBL
Match: A0A6J1FNX2 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111445964 PE=4 SV=1)

HSP 1 Score: 575 bits (1481), Expect = 3.38e-188
Identity = 335/623 (53.77%), Postives = 376/623 (60.35%), Query Frame = 0

Query: 47   LENLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIG 106
            LE+LKLL++AKNL+TLIIDA  +D QKV+NLI T+FL LRTL  +    +T +VLP SIG
Sbjct: 592  LESLKLLFKAKNLKTLIIDAVNEDCQKVVNLISTQFLCLRTLKVEFCYTNTSKVLPSSIG 651

Query: 107  KLKHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGN 166
            KLKHLRYL IC  +L+FLPNSITKLYNLE LILDWCE LQELPRDAKNWMKLRYLSL  N
Sbjct: 652  KLKHLRYLRICQRKLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLRYLSLDRN 711

Query: 167  TEIKFLPDSIASLQNLETLIL--------------------------------------- 226
              IKFLPDSIA+L NLETLIL                                       
Sbjct: 712  VNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKFLPDSIGA 771

Query: 227  ---------------------------------------------------------QDC 286
                                                                     QDC
Sbjct: 772  LHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLETLILQDC 831

Query: 287  SLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWLR-----DLSELN 346
            S LRELPKDIKKCVNLKHL+L GC  LTH+PKGLGELT+LQTMNL  L+     DLSELN
Sbjct: 832  SELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIGCDLSELN 891

Query: 347  RLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKG 406
            RL KL GSLTI+GLEVCT + LE S   L LKLG QEL+ VWNYS+D+ MNIVLDNECKG
Sbjct: 892  RLDKLEGSLTIEGLEVCTFDVLEKS---LPLKLGVQELELVWNYSEDQDMNIVLDNECKG 951

Query: 407  DLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHLV------------LPQFDQFPCLK 466
                LQPHSN+R IDIC YPEV LCDWLSSNT +HLV            LPQFDQFP LK
Sbjct: 952  GFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFDQFPFLK 1011

Query: 467  CLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDMHNLKGWWK---------------- 500
             L L+SLP IEYIDN+ Y SSS+FFPSLE+LSI++M NLKGWWK                
Sbjct: 1012 YLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESSPNNASFPI 1071

BLAST of Cp4.1LG04g08650 vs. ExPASy TrEMBL
Match: A0A6J1F802 (putative disease resistance RPP13-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111441703 PE=4 SV=1)

HSP 1 Score: 329 bits (843), Expect = 3.34e-103
Identity = 172/236 (72.88%), Postives = 194/236 (82.20%), Query Frame = 0

Query: 49  NLKLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIGKL 108
           + KLL+EAKNL+TLIIDAPT + +K LNLI TKFL+ RTL  + +  +  +VLPKSIGKL
Sbjct: 203 SFKLLFEAKNLRTLIIDAPTHNGKKALNLISTKFLQWRTLCLNFAYATPSEVLPKSIGKL 262

Query: 109 KHLRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGNTE 168
           KHLRYL I L+ L FLPNSITKL+NLETLI D C+ LQELPRDA+NWMKLRYLSL  N E
Sbjct: 263 KHLRYLKIMLFHLLFLPNSITKLHNLETLIFDGCKRLQELPRDAENWMKLRYLSLARNWE 322

Query: 169 IKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELT 228
           IK LPD IA+L NLET ILQDCSLLRELPK IKKCVN KHLDL GC+ALTHLPKGLGELT
Sbjct: 323 IKLLPDFIAALHNLETFILQDCSLLRELPKGIKKCVNFKHLDLHGCSALTHLPKGLGELT 382

Query: 229 NLQTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLG 279
           +LQTMNL  L+     DLSELNRL+KL+G LTIKGLEVCT +DLE + FNL LKLG
Sbjct: 383 SLQTMNLIVLKKDTGCDLSELNRLNKLKGKLTIKGLEVCTADDLEMNSFNLPLKLG 438

BLAST of Cp4.1LG04g08650 vs. ExPASy TrEMBL
Match: A0A0A0K5Z6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G425920 PE=4 SV=1)

HSP 1 Score: 303 bits (776), Expect = 3.03e-89
Identity = 212/521 (40.69%), Postives = 276/521 (52.98%), Query Frame = 0

Query: 51  KLLYEAKNLQTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPKSIGKLKH 110
           K L E KNL+TL    P         L+    LRLR+L+     +S FQ +PK I +L+H
Sbjct: 157 KSLTEVKNLRTL--HGPPF-------LLSENHLRLRSLNLG---YSKFQKIPKFISQLRH 216

Query: 111 LRYLNICLYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKNWMKLRYLSLTGNTEIK 170
           LRYL+I  ++++FLP  ITKLYNLETLIL                               
Sbjct: 217 LRYLDISDHDMKFLPKFITKLYNLETLIL------------------------------- 276

Query: 171 FLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNL 230
                            + CS LRELP DI   +NLKHLD+ GC  LTH+PKGLG LT+L
Sbjct: 277 -----------------RHCSDLRELPTDINNLINLKHLDVHGCYRLTHMPKGLGGLTSL 336

Query: 231 QTMNLCWLR-----DLSELNRLSKLRGSLTIKGLEVCTTEDLENSYFNLRLKLGFQELKF 290
           QTMNL  L      DLSELN L++LRGSL IKGLE+CTT DL+N+ + +  K G Q+LK 
Sbjct: 337 QTMNLFVLGKDKGCDLSELNELARLRGSLLIKGLELCTTTDLKNAKY-MEEKFGIQKLKL 396

Query: 291 VWNYSK-DEHMNIVLDNECKGDLNCLQPHSNVRKIDICRYPEVMLCDWLSSNTLLHLV-- 350
            WN    D   +   +N+ +  L+CL+PHSNV K+ I  Y  V LC+WLS + L  LV  
Sbjct: 397 RWNRDLYDAETDYASENDDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLSFDYLGGLVNI 456

Query: 351 ----------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEKLSIIDMHNL 410
                     LPQFDQFP LK L L++LP IEYIDN+   SSS FFPSLEKL+I+ M NL
Sbjct: 457 ELQSCEKLQHLPQFDQFPFLKHLLLENLPSIEYIDNNNSLSSSTFFPSLEKLTIMTMPNL 516

Query: 411 KGWWK------------------------------------------------VSLQLLN 470
           KGWWK                                                VS+QL +
Sbjct: 517 KGWWKGETPPESARYSALFPTILHHLSRLDISNCPQLASIPQHPPLRSLALNDVSVQLFD 576

Query: 471 VIIEKTANP----SSTPSKLSHVYLSKIDDLEFLPKELFYHFTNLLFLVIEDCKNLQLSS 500
           ++I+    P    SS  SKLS +++  ID LEFLP+ELF   T+L    + +CKNLQ+SS
Sbjct: 577 MVIKMATTPAADSSSALSKLSILHIQNID-LEFLPEELFGSTTDLEIFTVVNCKNLQMSS 615

BLAST of Cp4.1LG04g08650 vs. ExPASy TrEMBL
Match: A0A6J1DKY9 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021874 PE=4 SV=1)

HSP 1 Score: 306 bits (783), Expect = 8.13e-88
Identity = 217/550 (39.45%), Postives = 278/550 (50.55%), Query Frame = 0

Query: 31   LRKSRETISF-ICDDEVLENL-KLLYEAKNLQTLIIDAPTK----DAQKVLNLIPTKFLR 90
            L K    +SF +   E LE L + L EAKNL+T       +    +  ++   +    LR
Sbjct: 523  LSKRTHHVSFDLMSWEELERLPETLSEAKNLRTCFFHYNQRRTDNNFNEIFESVFYNLLR 582

Query: 91   LRTLSFDSSIFSTFQV-LPKSIGKLKHLRYLNICLYE----------------------- 150
            LRTL      F    V   K I KLKHLRYL I +Y                        
Sbjct: 583  LRTLDLQLLSFDKQNVKFLKCISKLKHLRYLEIQMYTKTEFFMNHITGLYNLEVFILRCT 642

Query: 151  -------------------------LQFLPNSITKLYNLETLILDWCEHLQELPRDAKNW 210
                                     ++FLPNS  +L NLETL+L  C  L+EL  D +NW
Sbjct: 643  RLKELPRDIKNWNELKHVYLYKNEIIEFLPNSFAELNNLETLVLHQCTKLKELLGDVRNW 702

Query: 211  MKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCN 270
            + LRYL L+ +  ++FLPDSI  L+NLETL+L+ CS L+ELP+D K  +NLKH+DL GC 
Sbjct: 703  INLRYLDLSSSG-VEFLPDSITQLKNLETLLLRFCSKLQELPRDTKNLINLKHVDLYGCW 762

Query: 271  ALTHLPKGLGELTNLQTMNLCWLRD------LSELNRLSKLRGSLTIKGLEVCTTEDLEN 330
            ALTH+PKGLGELT+LQ +N   L        LSELN L+KLRGSLTI  L  CTT DL  
Sbjct: 763  ALTHMPKGLGELTDLQILNFFVLEKDNNGGGLSELNELNKLRGSLTINRLNFCTTSDLPT 822

Query: 331  SYFNLRLKLGFQELKFVWNYSKDEHMNIV---LDNECKGDLNCLQPHSNVRKIDICRYPE 390
            +   L LK   QEL   W +  D         LD++C+  L CL PHSN+R + I +Y  
Sbjct: 823  NAKFLLLKSSLQELVLEWEWDNDSVNEPTIGDLDDDCESVLECLHPHSNLRNLCIDKYRG 882

Query: 391  VMLCDWLSSNTLLHLV------------LPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSS 450
            V LC+W+SSN L  LV            LPQFDQFP LK L L+ L  IEYI ND YR S
Sbjct: 883  VKLCNWVSSNFLGCLVTIKLSGCKRLQHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCS 942

Query: 451  SIFFPSLEKLSIIDMHNLKGWWKVSLQLLNVIIEKTANPSSTPSKLSHVYLSKIDDLEFL 500
            S  FPSLE+LSI  M NLK WWK           +T+  +S+ + L  +    +  LE  
Sbjct: 943  STLFPSLEELSIFCMPNLKRWWKKG---------QTSKATSSQNVLFPIIFPCLSKLEIW 1002

BLAST of Cp4.1LG04g08650 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.3e-26
Identity = 137/523 (26.20%), Postives = 208/523 (39.77%), Query Frame = 0

Query: 82   FLRLRTLSFDSSIFSTFQVLPKSIGKLKHLRYLNICLYELQFLPNSITKLYNLETLILDW 141
            FL L   +   S      V  K +  L  LR L++  Y++  LP                
Sbjct: 551  FLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLP---------------- 610

Query: 142  CEHLQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIK 201
                   P   KN    R+L L+  TE++ LP S+  + NL+TL+L  CS L+ELP DI 
Sbjct: 611  -------PDFFKNISHARFLDLS-RTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDIS 670

Query: 202  KCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWL-----RDLSELNRLSKLRGSLTI 261
              +NL++LDL G   L  +P+  G L +LQT+   ++       +SEL  L  L G L I
Sbjct: 671  NLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKI 730

Query: 262  KGLE-VCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKGDLNC---LQP 321
              L+ V    D   +  NL  K   +E+ FVW        N    +  + +      L+P
Sbjct: 731  VELQRVVDVADAAEA--NLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRP 790

Query: 322  HSNVRKIDICRYPEVMLCDWLSSNTLLHLV------------LPQFDQFPCLKCLGLKSL 381
            H ++ K+ I RY      DWLS  +   +V            LP   Q PCLK L +  +
Sbjct: 791  HRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGM 850

Query: 382  PCIEYIDNDGYRSSSIF-------FPSLEKLSIIDMHNLKGWWKV---------SLQLLN 441
              ++ I    Y S           F SLE L   ++ + + W  V         SL+ L 
Sbjct: 851  VGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTRGDLFPSLKKLF 910

Query: 442  VI--IEKTAN-PSSTPSKLS-HVYLSKIDDLEFLPKELFY-------------------- 501
            ++   E T   P+  PS +S H+Y  K   L+F P    Y                    
Sbjct: 911  ILRCPELTGTLPTFLPSLISLHIY--KCGLLDFQPDHHEYSYRNLQTLSIKSSCDTLVKF 970

Query: 502  ---HFTNLLFLVIEDCKNLQLSSALVQHPTNDVLWKEFQSLRVLFLRAIPKLEYLPNGMQ 541
               HF NL  L ++ C +L       +H       +  +      L+ +PKL  LP  +Q
Sbjct: 971  PLNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRINDCQNLQLLPKLNALPQNLQ 1030

BLAST of Cp4.1LG04g08650 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 109.8 bits (273), Expect = 7.9e-24
Identity = 75/181 (41.44%), Postives = 103/181 (56.91%), Query Frame = 0

Query: 83   LRLRTLSFDSSIFSTFQVLPKSIGKLKHLR--YLNICLYELQFLPNSITKLYNLETLILD 142
            + L+TL+   S  S+   LP SIG L +L+  YL+ C   L  LP+SI  L NL+ L L 
Sbjct: 932  INLKTLNL--SECSSLVELPSSIGNLINLQELYLSEC-SSLVELPSSIGNLINLKKLDLS 991

Query: 143  WCEHLQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDI 202
             C  L ELP    N + L+ L+L+  + +  LP SI +L NL+ L L +CS L ELP  I
Sbjct: 992  GCSSLVELPLSIGNLINLKTLNLSECSSLVELPSSIGNLINLQELYLSECSSLVELPSSI 1051

Query: 203  KKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWLRDLSELNRLSKLRGSLTIKGLE 262
               +NLK LDL GC++L  LP  +G L NL+T+N   L   S L  L    G+L +K L+
Sbjct: 1052 GNLINLKKLDLSGCSSLVELPLSIGNLINLKTLN---LSGCSSLVELPSSIGNLNLKKLD 1106

BLAST of Cp4.1LG04g08650 vs. TAIR 10
Match: AT2G14080.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 96.7 bits (239), Expect = 6.9e-20
Identity = 81/224 (36.16%), Postives = 113/224 (50.45%), Query Frame = 0

Query: 47  LENLKL--LYEAKNLQTLIIDAPTKDAQKVLNLIPTKFL---------RLRTLSFDSSIF 106
           L NLK   L+ +KNL+ L  D  +    +VLNL     L           + L  + S  
Sbjct: 673 LRNLKRMDLFSSKNLKEL-PDLSSATNLEVLNLNGCSSLVELPFSIGNATKLLKLELSGC 732

Query: 107 STFQVLPKSIGKLKHLRYLNICLYE-LQFLPNSITKLYNLETLILDWCEHLQELPRDAKN 166
           S+   LP SIG   +L+ ++    E L  LP+SI    NL+ L L  C  L+ELP    N
Sbjct: 733 SSLLELPSSIGNAINLQTIDFSHCENLVELPSSIGNATNLKELDLSCCSSLKELPSSIGN 792

Query: 167 WMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGC 226
              L+ L L   + +K LP SI +  NL+ L L  CS L +LP  I   +NL+ L L GC
Sbjct: 793 CTNLKKLHLICCSSLKELPSSIGNCTNLKELHLTCCSSLIKLPSSIGNAINLEKLILAGC 852

Query: 227 NALTHLPKGLGELTNLQTMNLCWLRDLSE-------LNRLSKLR 252
            +L  LP  +G+ TNL+ +NL +L  L E       L++LS+LR
Sbjct: 853 ESLVELPSFIGKATNLKILNLGYLSCLVELPSFIGNLHKLSELR 895

BLAST of Cp4.1LG04g08650 vs. TAIR 10
Match: AT3G25510.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 93.2 bits (230), Expect = 7.7e-19
Identity = 65/184 (35.33%), Postives = 94/184 (51.09%), Query Frame = 0

Query: 96  STFQVLPKSIGKLKHLRYLNIC-LYELQFLPNSITKLYNLETLILDWCEHLQELPRDAKN 155
           S+   LP SIG   +L+ L++     L  LP+ I    NLE L L  C  L E+P    +
Sbjct: 816 SSLVELPSSIGNAINLQNLDLSNCSSLVKLPSFIGNATNLEILDLRKCSSLVEIPTSIGH 875

Query: 156 WMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGC 215
              L  L L+G + +  LP S+ ++  L+ L L +CS L +LP       NL  LDL GC
Sbjct: 876 VTNLWRLDLSGCSSLVELPSSVGNISELQVLNLHNCSNLVKLPSSFGHATNLWRLDLSGC 935

Query: 216 NALTHLPKGLGELTNLQTMNLCWLRDLSELNRLSKLRGSL-TIKGLEVCTTEDLENSYFN 275
           ++L  LP  +G +TNLQ +NLC   + S L +L    G+L  +  L +   + LE    N
Sbjct: 936 SSLVELPSSIGNITNLQELNLC---NCSNLVKLPSSIGNLHLLFTLSLARCQKLEALPSN 995

Query: 276 LRLK 278
           + LK
Sbjct: 996 INLK 996

BLAST of Cp4.1LG04g08650 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 90.9 bits (224), Expect = 3.8e-18
Identity = 80/276 (28.99%), Postives = 125/276 (45.29%), Query Frame = 0

Query: 145 LQELPRDAKNWMKLRYLSLTGNTEIKFLPDSIASLQNLETLILQDCSLLRELPKDIKKCV 204
           +  LP+  K    LRYL L+ +T+IK LP+ + +L NL+TL+L +C  L  LPK I + +
Sbjct: 585 ITNLPKSLKGLKLLRYLDLS-STKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELI 644

Query: 205 NLKHLDLRGCNALTHLPKGLGELTNLQTMNLCWLRDLS-----ELNRLSKLRGSLTIKGL 264
           NL+ LDL G   L  +P G+ +L +LQ ++   +  LS     EL  LS LRG+L I  L
Sbjct: 645 NLRLLDLVG-TPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISEL 704

Query: 265 E-VCTTEDLENSYFNLRLKLGFQELKFVWNYSKDEHMN---IVLDNECKGDLNCLQPHSN 324
           + V    + +++   L+ K     L   W       +      L  + K  L  L+PH +
Sbjct: 705 QNVAFASEAKDA--GLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPH 764

Query: 325 VRKIDICRYPEVMLCDWLSSNTL------------LHLVLPQFDQFPCLKCLGLKSLPCI 384
           ++   I  Y       WL  ++             L + LP   Q P LK L ++    +
Sbjct: 765 LKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNIL 824

Query: 385 EYIDNDGY----RSSSIFFPSLEKLSIIDMHNLKGW 396
           + +  D +     S  + F SL+ L    M     W
Sbjct: 825 QKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEW 856