Cp4.1LG04g07470 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g07470
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	hemK methyltransferase family member 2-like
Location	Cp4.1LG04: 4152815 .. 4153522 (-)
RNA-Seq Expression	Cp4.1LG04g07470
Synteny	Cp4.1LG04g07470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATTGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGATATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGATGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGCGTTATCGATAGAATGTTGCCTGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAAGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTTATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGATTCAAGTCACAAAACAGGAACAATTTCAACATCTTAA

mRNA sequence

ATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATTGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGATATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGATGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGCGTTATCGATAGAATGTTGCCTGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAAGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTTATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGATTCAAGTCACAAAACAGGAACAATTTCAACATCTTAA

Coding sequence (CDS)

ATGTCCCCATTCAAAACTGCGCAAATCCACCTTGTGAGTTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCATTTGCTTTGGTTGATGCACTTTTAGCTGACCGAGTTAACTTGTTAAATCATCACCCAACATTGTGCTTAGAAGTAGGCTGTGGAAGTGGGTATGTCATAACATCTCTTGCTCTTATGTTGGGAAAGGAAGCCTCTGGCACTCACTATATTGCTACAGACATCAATCCTCATGCAACGAGAGTCACTCAGGAGACACTAGAAGCACATGGGGTTCATGCGGAGTTGATATCCACTGACATAGCATATGGTTTGGAGAAACGACTGGCTTCATCTGTTGATGTGTTGATTGTGAATCCACCCTATGTTCCAACCCCTGAGGATGAAGTGGGTCTTGAAGGAATTGCCTCTGCTTGGGCAGGAGGGGAAAATGGCCGCAGCGTTATCGATAGAATGTTGCCTGTTGCTGATGCTCTTTTGTCTGACAAAGGCTGGTTGTACATGGTTTTTCTTGAAGCAAACAATCCCTCCCAGATATGCCTTCAAATGAGAGAAAAAGGGTATGCTAGTCGAATCGTTGTGCAGAGATCAACTGAAGAAGAGAGTCTTCATGTTATCAAGTTTTGGAAGAATGCTGATCTTCAAGTTCATGGAAAAGATTCAAGTCACAAAACAGGAACAATTTCAACATCTTAA

Protein sequence

MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS

Homology

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BLAST of Cp4.1LG04g07470 vs. ExPASy Swiss-Prot
Match: Q6SKR2 (Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.4e-42
Identity = 91/200 (45.50%), Postives = 122/200 (61.00%), Query Frame = 0


Query: 16  REVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYI 75
           R+VYEP +D+F L+DAL A    L      +CLEVG GSG V   LA M+G  A    Y+
Sbjct: 20  RDVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGAGSGVVSAFLASMIGPRAL---YM 79

Query: 76  ATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEVG 135
            TDINP A   T ET   + VH + + TD+ +GL  RL   VD+L+ NPPYV TP +EVG
Sbjct: 80  CTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVG 139

Query: 136 LEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASR 195
             GI +AWAGG NGR V+DR  P+A  LLS +G  Y+V ++ NNP +I   M+ +G    
Sbjct: 140 SRGIEAAWAGGRNGREVMDRFFPLAPELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGT 199

Query: 196 IVVQRSTEEESLHVIKFWKN 216
             + R   +E+L V++F K+
Sbjct: 200 TALCRQAGQEALSVLRFSKS 214

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BLAST of Cp4.1LG04g07470 vs. ExPASy Swiss-Prot
Match: Q9Y5N5 (Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4)

HSP 1 Score: 172.6 bits (436), Expect = 5.4e-42
Identity = 91/199 (45.73%), Postives = 119/199 (59.80%), Query Frame = 0


Query: 17  EVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHYIA 76
           +VYEP +D+F L+DAL A    L      +CLEVG GSG V   LA M+G +A    Y+ 
Sbjct: 21  DVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGSGSGVVSAFLASMIGPQAL---YMC 80

Query: 77  TDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEVGL 136
           TDINP A   T ET   + VH + + TD+  GL  RL   VD+L+ NPPYV TP  EVG 
Sbjct: 81  TDINPEAAACTLETARCNKVHIQPVITDLVKGLLPRLTEKVDLLVFNPPYVVTPPQEVGS 140

Query: 137 EGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYASRI 196
            GI +AWAGG NGR V+DR  P+   LLS +G  Y+V ++ NNP +I   M+ KG     
Sbjct: 141 HGIEAAWAGGRNGREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTT 200

Query: 197 VVQRSTEEESLHVIKFWKN 216
            + R   +E+L V+KF K+
Sbjct: 201 ALSRQAGQETLSVLKFTKS 214

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BLAST of Cp4.1LG04g07470 vs. ExPASy Swiss-Prot
Match: Q9UT94 (eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mtq2 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.8e-22
Identity = 73/221 (33.03%), Postives = 114/221 (51.58%), Query Frame = 0


Query: 6   TAQIHLVSSHREVYEPCDDSFALVDALLADRVNL---LNHHPTLCLEVGCGSGYVITSLA 65
           T+Q+ L    ++VYEP +D+FAL+DAL  D   L         L  E+GCGSG   + L 
Sbjct: 7   TSQLRL-KEFQDVYEPAEDTFALLDALEKDAKKLRQMAEMKNLLTAEIGCGSGCASSFLK 66

Query: 66  LMLGKEASGTHYIATDINPHATRVTQET-LEAHGVHAE------LISTDIAYGLEKRLAS 125
             + K     H++ +DI+  A R ++ T L    ++ +       + T    G+  RL +
Sbjct: 67  SGILKNKPIVHFM-SDISNSACRASKITALNNRELYKDDNGLFITVQTSFLDGI--RLGN 126

Query: 126 SVDVLIVNPPYVPTPEDEVGLEG--IASAWAGGENGRSVIDRMLPVADALLSDKGWLYMV 185
            VD+LI NPPYVPT  +E+  E   IASAWAGG +G  V   +L     +LS  G  YMV
Sbjct: 127 GVDILIFNPPYVPTEFEEIPSEAATIASAWAGGTDGMDVTSTLLNQLKDILSQDGVFYMV 186

Query: 186 FLEANNPSQICLQMREKGYASRIVVQRSTEEESLHVIKFWK 215
            +  N    IC  +++ G+     ++R    E+L +++ ++
Sbjct: 187 AVARNKLHSICEILQKDGFIVNETLKRKAGRETLSILRIYR 223

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BLAST of Cp4.1LG04g07470 vs. ExPASy Swiss-Prot
Match: Q58338 (Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0928 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.8e-16
Identity = 55/200 (27.50%), Postives = 94/200 (47.00%), Query Frame = 0


Query: 15  HREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLALMLGKEASGTHY 74
           H EVYEP +DS  L+        NL++      LE+G G+G +  + A    K+  G   
Sbjct: 13  HPEVYEPAEDSILLLK-------NLVDVKNKDVLEIGVGTGLISIACAKKGAKKIVG--- 72

Query: 75  IATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIVNPPYVPTPEDEV 134
              DINP+A ++ +E  + + V+     +D    L + +    DV++ NPPY+PT EDE 
Sbjct: 73  --VDINPYAVKLAKENAKLNNVNISFFESD----LFENVTGKFDVILFNPPYLPTSEDEK 132

Query: 135 GLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQICLQMREKGYAS 194
               +  A+ GG++GR ++DR +      L   G + ++        +   +++  G+  
Sbjct: 133 IDSYLNFAFDGGKDGREILDRFIYELPNYLKKGGVVQILQSSLTGEKETINKLKPLGFKV 192

Query: 195 RIVVQRSTEEESLHVIKFWK 215
            I  +     E L VI  W+
Sbjct: 193 EISARLKVPFEELMVINAWR 196

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BLAST of Cp4.1LG04g07470 vs. ExPASy Swiss-Prot
Match: Q03920 (eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MTQ2 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.3e-15
Identity = 56/180 (31.11%), Postives = 87/180 (48.33%), Query Frame = 0


Query: 17  EVYEPCDDSFALVDALLADRVNL---LNHHPTLCLEVGCGSGYVITSLALMLGKEASGTH 76
           +VYEP +DSF ++D L  +   L     +   +  E+G GSG V T L          + 
Sbjct: 13  KVYEPAEDSFLILDCLEKEHDFLKQKFGNRLAIVCEIGSGSGIVTTFLMQNKIIPQENSI 72

Query: 77  YIATDINPHATRVTQETLEAHGVHA---ELISTDIAYGLEKRLASSVDVLIVNPPYVPTP 136
           ++A DINP A   T +T + +   +   E+I  D+   +     + VDVLI NPPYVP  
Sbjct: 73  HLAVDINPWALEATLDTAKLNSCKSSFLEVIQADLNSSIRN---NQVDVLIFNPPYVPAE 132

Query: 137 --EDEVGLEGIASAW-----AGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQI 184
              D  G    A  W      GG++G ++ D++L   + +LS  G  Y++F   N P ++
Sbjct: 133 CVPDVPGSREEADQWLDLALLGGKDGMAITDKLLRQLEQILSPDGVAYILFCARNKPKEV 189

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BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: XP_023530050.1 (hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo] >XP_023530051.1 hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477 bits (1227), Expect = 8.64e-170
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235

[Close]

BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: XP_022988929.1 (hemK methyltransferase family member 2-like [Cucurbita maxima] >XP_022988930.1 hemK methyltransferase family member 2-like [Cucurbita maxima])

HSP 1 Score: 474 bits (1221), Expect = 7.10e-169
Identity = 234/235 (99.57%), Postives = 234/235 (99.57%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235
           SQICLQMREKGYASRIVVQR TEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS
Sbjct: 181 SQICLQMREKGYASRIVVQRLTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235

[Close]

BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: XP_022927839.1 (hemK methyltransferase family member 2-like [Cucurbita moschata] >XP_022927840.1 hemK methyltransferase family member 2-like [Cucurbita moschata])

HSP 1 Score: 473 bits (1217), Expect = 2.89e-168
Identity = 232/235 (98.72%), Postives = 234/235 (99.57%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAEL+STDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           IVNPPYVPTPE+EVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG ISTS
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGAISTS 235

[Close]

BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: KAG7022515.1 (HemK methyltransferase family member 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 452 bits (1163), Expect = 3.37e-159
Identity = 221/223 (99.10%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 22  MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 81

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAEL+STDIAYGLEKRLASSVDVL
Sbjct: 82  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 141

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           IVNPPYVPTPE+EVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 142 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 201

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 223
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK
Sbjct: 202 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGK 244

[Close]

BLAST of Cp4.1LG04g07470 vs. NCBI nr
Match: XP_023553679.1 (hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 433 bits (1113), Expect = 4.42e-152
Identity = 211/230 (91.74%), Postives = 220/230 (95.65%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLA+RVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASG HYIATDINPHA RVTQETLEAHGVHAEL+STDIA GLEKRLA SVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 230
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GK+S+HKTG
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKESTHKTG 230

[Close]

BLAST of Cp4.1LG04g07470 vs. ExPASy TrEMBL
Match: A0A6J1JKY8 (hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486137 PE=3 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 3.44e-169
Identity = 234/235 (99.57%), Postives = 234/235 (99.57%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235
           SQICLQMREKGYASRIVVQR TEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS
Sbjct: 181 SQICLQMREKGYASRIVVQRLTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235

[Close]

BLAST of Cp4.1LG04g07470 vs. ExPASy TrEMBL
Match: A0A6J1EJ44 (hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC111434608 PE=3 SV=1)

HSP 1 Score: 473 bits (1217), Expect = 1.40e-168
Identity = 232/235 (98.72%), Postives = 234/235 (99.57%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAEL+STDIAYGLEKRLASSVDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELVSTDIAYGLEKRLASSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           IVNPPYVPTPE+EVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP
Sbjct: 121 IVNPPYVPTPEEEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGTISTS 235
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG ISTS
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTGAISTS 235

[Close]

BLAST of Cp4.1LG04g07470 vs. ExPASy TrEMBL
Match: A0A6J1I5Y4 (hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470989 PE=3 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 7.15e-151
Identity = 210/230 (91.30%), Postives = 219/230 (95.22%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLA+RVNLLNHHPTLCLEVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASG HYIATDINPHA RVTQETLEAHGVHAEL+STDIA GLEKRLA SVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+L VAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILLVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 230
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GK+S+HKTG
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKESTHKTG 230

[Close]

BLAST of Cp4.1LG04g07470 vs. ExPASy TrEMBL
Match: A0A6J1GME1 (hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455633 PE=3 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 7.15e-151
Identity = 209/230 (90.87%), Postives = 218/230 (94.78%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLA+RVNLLNHHPTLC EVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCFEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASG HYIATDINPHA RVTQETLEAHGVHAEL+STDIA GLEKRLA SVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG +GIASAWAGGENGRSVIDR+LPVAD LLSDKGWLYMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 230
           SQICLQMREKGYASRIVVQRSTEEESLHVIKFWK+ADLQV GK+S+HK G
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKESTHKIG 230

[Close]

BLAST of Cp4.1LG04g07470 vs. ExPASy TrEMBL
Match: A0A6J1DPY1 (hemK methyltransferase family member 2 OS=Momordica charantia OX=3673 GN=LOC111022463 PE=3 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 4.28e-150
Identity = 207/230 (90.00%), Postives = 218/230 (94.78%), Query Frame = 0


Query: 1   MSPFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITS 60
           MS F+TAQIHLVSSHREVYEPCDDSFALVDALLADR NLLNH+PTLCLEVGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRANLLNHNPTLCLEVGCGSGYVITS 60

Query: 61  LALMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVL 120
           LALMLGKEASGTHYIATDINPHA RVTQETLEAHGVHAEL+STDIA G+EKRLA  VDVL
Sbjct: 61  LALMLGKEASGTHYIATDINPHALRVTQETLEAHGVHAELVSTDIASGMEKRLAGIVDVL 120

Query: 121 IVNPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNP 180
           +VNPPYVPTPEDEVG EGIASAWAGGENGRSVIDR+LPVAD LLSDKGW YMV L ANNP
Sbjct: 121 VVNPPYVPTPEDEVGREGIASAWAGGENGRSVIDRILPVADVLLSDKGWFYMVTLTANNP 180

Query: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSSHKTG 230
           SQICLQMREKGYASRI+VQRSTEEESLHVIKFWK+ADLQV GK+S+H+TG
Sbjct: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKESTHRTG 230

[Close]

BLAST of Cp4.1LG04g07470 vs. TAIR 10
Match: AT3G13440.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.0e-91
Identity = 157/224 (70.09%), Postives = 190/224 (84.82%), Query Frame = 0


Query: 3   PFKTAQIHLVSSHREVYEPCDDSFALVDALLADRVNLLNHHPTLCLEVGCGSGYVITSLA 62
           P + A I LVSSHREVYEPCDDSFALVDALLADR NL+ H+P +C+E+GCGSGYVITSL 
Sbjct: 34  PPRIAAIRLVSSHREVYEPCDDSFALVDALLADRTNLIEHNPKICMEIGCGSGYVITSLI 93

Query: 63  LMLGKEASGTHYIATDINPHATRVTQETLEAHGVHAELISTDIAYGLEKRLASSVDVLIV 122
           L+L  E  G HY+A D NP ATRVT+ETLEAHGV+A++I  D+A GLEKRLA SVDV++V
Sbjct: 94  LLLQNEVPGVHYLAIDTNPIATRVTKETLEAHGVNADVICADLATGLEKRLAGSVDVIVV 153

Query: 123 NPPYVPTPEDEVGLEGIASAWAGGENGRSVIDRMLPVADALLSDKGWLYMVFLEANNPSQ 182
           NPPYVPTPE EVG+EGIASAWAGGENGR+VID++LPV D LLS+KGW Y+V L +N P++
Sbjct: 154 NPPYVPTPEYEVGMEGIASAWAGGENGRTVIDKILPVVDLLLSEKGWFYLVTLTSNFPAE 213

Query: 183 ICLQMREKGYASRIVVQRSTEEESLHVIKFWKNADLQVHGKDSS 227
           ICL MR+KGYASRIVVQRSTEEE+L ++KFW++ D +   K++S
Sbjct: 214 ICLMMRKKGYASRIVVQRSTEEENLVILKFWRDKDEESQDKETS 257

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g07470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6SKR2	1.4e-42	45.50	Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1	[more]
Q9Y5N5	5.4e-42	45.73	Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4	[more]
Q9UT94	4.8e-22	33.03	eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (stra...	[more]
Q58338	8.8e-16	27.50	Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jan...	[more]
Q03920	4.3e-15	31.11	eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strai...	[more]

BLAST of Cp4.1LG04g07470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530050.1	8.64e-170	100.00	hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo] >XP_023...	[more]
XP_022988929.1	7.10e-169	99.57	hemK methyltransferase family member 2-like [Cucurbita maxima] >XP_022988930.1 h...	[more]
XP_022927839.1	2.89e-168	98.72	hemK methyltransferase family member 2-like [Cucurbita moschata] >XP_022927840.1...	[more]
KAG7022515.1	3.37e-159	99.10	HemK methyltransferase family member 2, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_023553679.1	4.42e-152	91.74	hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cp4.1LG04g07470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKY8	3.44e-169	99.57	hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1EJ44	1.40e-168	98.72	hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1I5Y4	7.15e-151	91.30	hemK methyltransferase family member 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1GME1	7.15e-151	90.87	hemK methyltransferase family member 2-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1DPY1	4.28e-150	90.00	hemK methyltransferase family member 2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Cp4.1LG04g07470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13440.1	1.0e-91	70.09	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 16..221 e-value: 2.8E-42 score: 146.2
None	No IPR available	PANTHER	PTHR45875	METHYLTRANSFERASE N6AMT1	coord: 5..226
None	No IPR available	PANTHER	PTHR45875:SF5	BNAC01G37640D PROTEIN	coord: 5..226
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 48..177 e-value: 1.61219E-8 score: 49.3507
IPR007848	Methyltransferase small domain	PFAM	PF05175	MTS	coord: 43..173 e-value: 1.2E-9 score: 38.0
IPR004557	Eukaryotic/archaeal PrmC-related	TIGRFAM	TIGR00537	TIGR00537	coord: 18..213 e-value: 4.2E-27 score: 93.0
IPR002052	DNA methylase, N-6 adenine-specific, conserved site	PROSITE	PS00092	N6_MTASE	coord: 120..126
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 26..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g07470.1	Cp4.1LG04g07470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006479	protein methylation
biological_process	GO:0032259	methylation
cellular_component	GO:0035657	eRF1 methyltransferase complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008276	protein methyltransferase activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity