Cp4.1LG04g06630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g06630
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionTransmembrane emp24 domain-containing protein
LocationCp4.1LG04: 4872675 .. 4878028 (+)
RNA-Seq ExpressionCp4.1LG04g06630
SyntenyCp4.1LG04g06630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAAATTGGGAGTATAAGTTAGCATGGCGGCGTACCACCGAAATTCTTCCAGTTTTCTAAAAACATCGTGCAAAAGAGAGTTGAATTGGAAACGAAGCAAGAGTCAAAGTAAAGATTCAAGAACAGTGCGAGAGAAATAGAGAGAATCGGAGAAGACAGCAATGGAGCGGCGGAGGATGATTCTGCCTTCTTTGCTGTTGATTTTGAGCTTTTTGGCGGCGGATCTTGTGCGCCCCACCCATGCTGTTTGGTTCAACCTTCCTTCTACGGGAACCAAGTGTGTGTCTGAGGAAATCCATAACAACGTCGTTGTTTTGGCTGATTATGTTGTGATCTCTGAGAATTATACTCATGCGCCCACCGTCTCCGTCAAGGTGAACTCCCTCTGCTATCTTTTTCCCCTGGATCGGTCAACGCTAGGGTTTTTCAATGATCTTATTTTATTTTCTTTCTGGATTTGTCTGGTTAATTGAAGAATCACTGCGATGAACTGAAATTACGGAATATTTTGATTGCTTATCTTGGACGGATCGAATACAGATCTTTTCGTTTTTCTCAGATTTTGTAGTGTTGTATTATGCACGAATATGGTGGAAAATGGATGTTATTGGTGCCGGGTGGGCGTTGGGTGCTGTTAGAAACATCCACGTTCACATACATTCAATAGTAGACTGACTGTTAAAGGACAATGTGATTGTTGGAACAGTGGGGCTTCTTCTTTGTGCCTGTTGGGTTCTCTTCACTTGAGTTTTAGTTCTTGTGATATAATTGATTTCGTTTTGTGGCTCTTCCCTTAGTCACTTGGGCTTTTGTAGATTTTAAAGTTAACTTCCACCCAGCTCGTTTGTTTCTCTTGAAATTAGAAGAATCACCAGATTTATTTTGTTTGATTGCCAAATATTTCACATTTATCTTGAACGGATTTGAAGGAATATTCGAAGACATGATAAATCAGATCTTTTCCCTGCCGATGTCTTGATCTATGTTTTTGTATCATTGGCTTAATATTCCATTTGTATATTTTCCCTCTCTAAGGTAACATCACCATACGGGAACAACCTCTACCACAAGGAGAATCAAACAAATGGGCAGTTTGCATTCACCACAGCAGAGGCTGGCAACCATTTGGCATGCTTTTGGATTGATGGCCATCATGAGCGTGGTGGTGATGTTAGCTTAAGTCTTGAATGGAGAACTGGAATTACAGCTAAGGACTGGGAATCAGTTGCAAAAAAGGAAAAAATTGAGGTGCGTTACACTCTTAAACTGAGGCATACACTTCCAAGATATGTTGACATTGACTTGCCATTTACTGGATGGTTGAAAAAAGTATCTATTTAGAGATTTACTTACTATAGTATTACAAAGCCACTGAAGTCTTATTGATATTTGTTTGCATATCAATCCAATTGTAACACATGGTTTATGTATGTGCCAAGCCCTTCGTTCTCTCACAAAATAGAATTGACGTGGTTAAAATGCCAACATTAAGACAGTGAACCTGCTGATTATGCAGCTCAGCTATGATATTTTTTTTGAAGTTGGTACACTTAAAAGGCTTTTAGAAAGGGACATAACAGAACTTCAGTGTTGAATATTTGGTCTTTAACTGAAGTTCTGAATCATGTATCATGACTGTAATAACTCTAAAGAACATCCAATGTATGGACTAAAGATAGAAAATCTTGGATTGAGGCTTTACTCTTCCTCTTTTAAATTTCATTCATTGATGAAATGTTTTGTTTCTTCTAAAAAGAAACGGCTAGCATCTTCGTATTTTTGTTTGTCACTCCTTTTTCATTTTCTGGAGTCATCTTTTGACTGGTCTAATTTTAGTTTATCCAATTGTTCAGTTTTAACGTAGGTTTCATTTGAATAATTTTGTGCTCGGTGGGAGTTAGTCGGTAAAGGGATGTTCTTACAAAAAATAAGCACATTTTTTTGGTTGCATATGGCTGAGTTAATTTTTTTGAGGTGGACTAGGATGCAATAGCTATTTGGAGTTTTGACTTGAGGTGTGATCAAATCAAGATTTAACAACTAATAGAATTTGTTTGCTTTTAATAATAATAAAATTGAGAGCTTGATTGTGTTGGGAATTAGATACTTGTGTCATACACGCCCCAAAAATGGATTTATAAAAAATCTCAATTGGAAAAAGAAAATATCACAGTACCATTATAGTTATACAAATCTTTTGACAAATTACACGAAGTAGAGAGGAGTCTAAATCCAAGATTCCAACCTCAGTGTTCCTCATACTGCTGTTCCACATACCACCGATCCACAAAGAAGCCAATGGAACCAACAATTTTGTTAAAGGCAACTGCAAGGGAATATCAAGGACCCAAAAATCCTCCTAAAACAAGATTATATCTCCCTTTACAAAGAAGAAAATTTTCACCTGAAACAGTGTTTCTTGAGACAGGGCTACATGCTTTTGGAGTTTGAAGCACCTCTAGCTTGCACTATCCAATTACCTGAGACTAACTAGTGTTTACTATCTTTGTAGCGACTAGGCCTGATTCTAGTGAGTAGGACCCTGTTTATAGTGAGTAGGACCCTTCTAGCTGCTGATTAGTTCCCCTTCACTTGTGAAGGAGAGGTTTCCATTCCACGGCAGTTTTGTCAACAAATATATTTTGGGTCGGAAACTTCGTTCTCAAAAGAGGGGATGCAGTCGTCATGATTTCCTTCTTAGGGCATTAATTTCTATCTAAAGTTTCTAATCTCAGTCATTTAGCAAGGGTGCTTAAAAATAGGGGGAGTTAAAAGTCCAGACCTCTCAAGTGCTAGTGGTTGGGTATTATTGTTTCATTTTATATTGTAAACAACTTTGAGTTTATTTTCAATATCGGAGTCCTATCAATTGCTGGTCTTCTTGGGCTTGTAATACCAATTTAAGAAGCGCCTTGTGTTCTTGGAAAGTGTGGCTACGAACAAGAATACCTTAGAAGAGTGCTAAAGCACTTGGTTTGAGACTTTAGAAGTAAATGATTTTATCCTTGTCCAACTTGTTGATCTCCTTTCCCCAGGTGCAATTAGGACAATGGACACTGACTTCCCTTGATATTGATATGTTCCATATTTAGGTAGACTATTCCTCTTTTCCATCCCGTGCACTGCCTGCATACTTAGTACCGGTCTGATTGGATCAAACATTCAAACCTTGACTTGTACTTCTACCTTCAACCATTCACAAAACTACTCTCCAAGACTTCCTTTGATATATCAGACAAAAACACGACAAAGATTTCAGAAAGCTTCGAATACTCTCTCTTTTTGGATAAGAAAATTTCATTGATAGTATGAAATTACAAAAGATGGGGAGAACCCAAAAGGATAGTTACAAAAGACCTCTCCAATTGGTTTAGAGGGTAGCAAAGCTATAGGAATGGAATGGAAGAGAGTATTTACACCAAGTCACGACTGTATTAATTATAGCCTAAAAAAATCTATAGTAGGATAATTCCTTTTCTTGAAAGATTCTTCTGTTTCTTTCTCACCAAATGTTCCAGCAAAAAGCTCTAATAGATTTCTCCCATAAAATCTTCTTCGTCCTTGTGAAAGAGTGTCCTCCATGGGTGAATTAAGGGAGAAGATGTGGATTTTCTGAGAGGACCATATGCCAGTTGAATGTCGAGCTTCCAATCCTCTCTCATGCTGCCTTCTTGTCATACAGCAACAACTTTCTGCACTGGGATTCTTCTTTGAGGAAGGAAATTGAGATAGTAGTAGAGCTTTAATTTCTACCCAAGTCTGAATTGCTCTTCCATAATCAGCCCATTGGAACCCTTTTGCTTCTGAAAAAAAAAAAAAAAAATCTCACATTCATTTCCAAGGAATCCTTTGGACAACATTCCGGAGGGCACTCAATCCTTATTGCACATCAGCTAGCACTACTGTTGTTTTGTGTTTGTGTGTGTGTGTGTGTTTACTTTATTTTTTTATTGGGATACAGAGAAAGGTACTATTAACAAGAGGAACCAAACAAAAGAAGGGAGATGAGGTATCATCCCCACAAGGCCAAAGGGTTAAAAAATGAGTCCGAATTGGCCAGAATCTATGCAAGATCGTAATTACAAAAAATTAATCCAAGGAACACAAGTGGAAGCATATAACATAGAGAAGCGAGCACCATTTCCTCCTCTGCCGTGGTTTCATCTTTTGTTCCTTTGTCAATACTAATGAATTTCTTAGCTTGCCTAACGTAACGAGGAGGGAGTTCAGGGTTCTCTAGTGAGCATAAACGAGAGAGAGAGGTTTAAGACTGTCTAACAAGCATGAACCAGAAAGAGATGGACATTCCAAGATGGATTGGAGGGATATGGTTTGAGGTCTAACGCTGAATCAGAGTAAGAGGATTAGATGAGAGCAATGGTTGAAGACCATGAGGGATGGAGACTGAAGACTAGGAGAGAACTCACTTGGATTAGGGTGGAAAATTATGTTTTCGACCAAGCATCATGTCCATAAACTTCAACCATTTTTTTTTTATAAAACGAACACCAATGCAGTTGCCTCAATAGCTTGTCAAAACATTTCGATAGGCGTCTTAGTGCGTGCTTTTGCAATGATTCCTCAACTGAATCAAAACAAGGCTCTGTATTTGCATGTGTATGCGCTTGACGTCTAAGCACATTTCTAAATCACTAACTAAGATGATAAGTTTCTATTCTTAGATTTACCACTGAGAATTTCTCTTATATTTCTGCATTTTATATATCTTCTCTTCCATAACCTCGAAGTCCTTTCCAAATCGCTATCTTGTAGGGCGTTGAGCTTGAGCTCCGGAAACTCGAAGGCGTGGTAGAGGCTATTCATGAGAACATTTTGTACCTAAAAGAAAGGTAATCTACGATGGAAAAACTATTCAAATTCTCTACAAAATTTTCTCCATGAATGTTTTGAGTAACTATTTGGTGTAATGTATATATGAACCACACAGGGAAGCAGAAATGAGGATAGTAAGTGAGAAAACAAATGGAAGAGTGGCGTGGTTTAGCATAATGTCTTTGGGTGTTTGTATTTTGGCTTCAACACTTCAACTGTGGTACTTGAAGCGATTCTTCCAGAAAAAGAAGCTCATTTAAAACAGTAATCCATCTTCTTTCTTGTTCTCTGAATTTCTTTTCTTACAATCTAGCATCACACAAATCTTATAGTTACAGAATTGTTTCATCACGGATTTTCGTTTAAATTCATTATCTTATGAGCCTTAGGTACCTCGATAGTCTGCGATGTCGAATATTACTTATTTGTTACTCATTCAAAACGCGTCTTATTTCTAAGCAATCTAACATATTACCAGTTTATCTATGATAGGATATACCAAATTTAGTTGTGTTGCTTG

mRNA sequence

TGAAAATTGGGAGTATAAGTTAGCATGGCGGCGTACCACCGAAATTCTTCCAGTTTTCTAAAAACATCGTGCAAAAGAGAGTTGAATTGGAAACGAAGCAAGAGTCAAAGTAAAGATTCAAGAACAGTGCGAGAGAAATAGAGAGAATCGGAGAAGACAGCAATGGAGCGGCGGAGGATGATTCTGCCTTCTTTGCTGTTGATTTTGAGCTTTTTGGCGGCGGATCTTGTGCGCCCCACCCATGCTGTTTGGTTCAACCTTCCTTCTACGGGAACCAAGTGTGTGTCTGAGGAAATCCATAACAACGTCGTTGTTTTGGCTGATTATGTTGTGATCTCTGAGAATTATACTCATGCGCCCACCGTCTCCGTCAAGGTAACATCACCATACGGGAACAACCTCTACCACAAGGAGAATCAAACAAATGGGCAGTTTGCATTCACCACAGCAGAGGCTGGCAACCATTTGGCATGCTTTTGGATTGATGGCCATCATGAGCGTGGTGGTGATGTTAGCTTAAGTCTTGAATGGAGAACTGGAATTACAGCTAAGGACTGGGAATCAGTTGCAAAAAAGGAAAAAATTGAGGGCGTTGAGCTTGAGCTCCGGAAACTCGAAGGCGTGGTAGAGGCTATTCATGAGAACATTTTGTACCTAAAAGAAAGGGAAGCAGAAATGAGGATAGTAAGTGAGAAAACAAATGGAAGAGTGGCGTGGTTTAGCATAATGTCTTTGGGTGTTTGTATTTTGGCTTCAACACTTCAACTGTGGTACTTGAAGCGATTCTTCCAGAAAAAGAAGCTCATTTAAAACAGTAATCCATCTTCTTTCTTGTTCTCTGAATTTCTTTTCTTACAATCTAGCATCACACAAATCTTATAGTTACAGAATTGTTTCATCACGGATTTTCGTTTAAATTCATTATCTTATGAGCCTTAGGTACCTCGATAGTCTGCGATGTCGAATATTACTTATTTGTTACTCATTCAAAACGCGTCTTATTTCTAAGCAATCTAACATATTACCAGTTTATCTATGATAGGATATACCAAATTTAGTTGTGTTGCTTG

Coding sequence (CDS)

ATGGAGCGGCGGAGGATGATTCTGCCTTCTTTGCTGTTGATTTTGAGCTTTTTGGCGGCGGATCTTGTGCGCCCCACCCATGCTGTTTGGTTCAACCTTCCTTCTACGGGAACCAAGTGTGTGTCTGAGGAAATCCATAACAACGTCGTTGTTTTGGCTGATTATGTTGTGATCTCTGAGAATTATACTCATGCGCCCACCGTCTCCGTCAAGGTAACATCACCATACGGGAACAACCTCTACCACAAGGAGAATCAAACAAATGGGCAGTTTGCATTCACCACAGCAGAGGCTGGCAACCATTTGGCATGCTTTTGGATTGATGGCCATCATGAGCGTGGTGGTGATGTTAGCTTAAGTCTTGAATGGAGAACTGGAATTACAGCTAAGGACTGGGAATCAGTTGCAAAAAAGGAAAAAATTGAGGGCGTTGAGCTTGAGCTCCGGAAACTCGAAGGCGTGGTAGAGGCTATTCATGAGAACATTTTGTACCTAAAAGAAAGGGAAGCAGAAATGAGGATAGTAAGTGAGAAAACAAATGGAAGAGTGGCGTGGTTTAGCATAATGTCTTTGGGTGTTTGTATTTTGGCTTCAACACTTCAACTGTGGTACTTGAAGCGATTCTTCCAGAAAAAGAAGCTCATTTAA

Protein sequence

MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Homology
BLAST of Cp4.1LG04g06630 vs. ExPASy Swiss-Prot
Match: Q6IDL4 (Transmembrane emp24 domain-containing protein p24delta3 OS=Arabidopsis thaliana OX=3702 GN=At1g09580 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-73
Identity = 138/213 (64.79%), Postives = 171/213 (80.28%), Query Frame = 0

Query: 3   RRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENY 62
           + R   P+  L+L FL   ++    AVW ++P TGTKCVSEEI +NVVVLADY++ISE++
Sbjct: 6   KMRREFPTAFLLL-FLVPVMIPVGEAVWLDVPPTGTKCVSEEIQSNVVVLADYLIISEDH 65

Query: 63  THAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLE 122
              PT+SVKVTSPYGNNL++ EN T+GQFAFTT E+GN+LACFW D       +VS++++
Sbjct: 66  EVMPTISVKVTSPYGNNLHNMENVTHGQFAFTTQESGNYLACFWADEKSHGNKNVSINID 125

Query: 123 WRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGR 182
           WRTGI AKDW S+AKKEKIEGVELE+RKLEG VEAIHENILYL+ REA+MR +SEKTN R
Sbjct: 126 WRTGIAAKDWASIAKKEKIEGVELEIRKLEGAVEAIHENILYLRNREADMRTMSEKTNSR 185

Query: 183 VAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
           VAW+SIMSLGVCI  S  Q+ YLK++F+KKKLI
Sbjct: 186 VAWYSIMSLGVCIAVSGFQVLYLKQYFEKKKLI 217

BLAST of Cp4.1LG04g06630 vs. ExPASy Swiss-Prot
Match: Q9FVU0 (Transmembrane emp24 domain-containing protein p24delta4 OS=Arabidopsis thaliana OX=3702 GN=CYB PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 3.9e-71
Identity = 133/212 (62.74%), Postives = 170/212 (80.19%), Query Frame = 0

Query: 4   RRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYT 63
           ++ ++P+ +L LS L   L     AVW  +P TG+KCVSEEI +NV+VLADY+VISE ++
Sbjct: 2   KKKMIPTTIL-LSALIFSLSPICEAVWLTVPHTGSKCVSEEIQSNVIVLADYLVISEEHS 61

Query: 64  HAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLEW 123
             PTVSVKVT+PYG  L+H+EN TNGQFAFTT E+G +LACF  D       D S++++W
Sbjct: 62  IFPTVSVKVTAPYGTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNKDFSINIDW 121

Query: 124 RTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRV 183
           +TGI AKDW+S+A+KEKIEGVELE +KLEG VEAIHEN++YL+ REAEMRIVSEKTN RV
Sbjct: 122 KTGIAAKDWDSIARKEKIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRV 181

Query: 184 AWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
           AW+SIMSLG+CI+ S LQ+ YLK++F+KKKLI
Sbjct: 182 AWYSIMSLGICIVVSGLQILYLKQYFEKKKLI 212

BLAST of Cp4.1LG04g06630 vs. ExPASy Swiss-Prot
Match: Q8RWM6 (Transmembrane emp24 domain-containing protein p24delta5 OS=Arabidopsis thaliana OX=3702 GN=At1g21900 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.0e-67
Identity = 132/207 (63.77%), Postives = 161/207 (77.78%), Query Frame = 0

Query: 11  LLLILSFLAADLVRPTHAVWFNLPST-GTKCVSEEIHNNVVVLADYVVISE-NYTHAPTV 70
           L ++L FL    V    A+W  +P+T GTKCVSEEI +NVVVLADY V+ E N  + P V
Sbjct: 13  LTVVLFFLT---VNYGEAIWLTIPTTGGTKCVSEEIQSNVVVLADYYVVDEHNPENTPAV 72

Query: 71  SVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLEWRTGIT 130
           S KVTSPYGNNL+H+EN T+GQFAFTT EAGN+LACFWID  H     ++L ++W+ GI 
Sbjct: 73  SSKVTSPYGNNLHHQENVTHGQFAFTTQEAGNYLACFWIDSSHHLANPITLGVDWKMGIA 132

Query: 131 AKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRVAWFSI 190
           AKDW+SVAKKEKIEGVEL+LR+LEG+V +I EN+ Y+K+REAEMR VSE TN RVAWFSI
Sbjct: 133 AKDWDSVAKKEKIEGVELQLRRLEGLVLSIRENLNYIKDREAEMREVSETTNSRVAWFSI 192

Query: 191 MSLGVCILASTLQLWYLKRFFQKKKLI 216
           MSLGVC++    Q+ YLKR+F KKKLI
Sbjct: 193 MSLGVCVVVVGSQILYLKRYFHKKKLI 216

BLAST of Cp4.1LG04g06630 vs. ExPASy Swiss-Prot
Match: F4J4Y0 (Transmembrane emp24 domain-containing protein p24delta6 OS=Arabidopsis thaliana OX=3702 GN=At3g10780 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.2e-49
Identity = 111/215 (51.63%), Postives = 140/215 (65.12%), Query Frame = 0

Query: 7   ILPSLLLILSFLA--ADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYTH 66
           I P L + L +LA    L     A+W  +P +G +CV EEI  NVVV+ DY+ I + +T 
Sbjct: 3   ISPVLFIGLIYLAGGGSLFPGVEAIWLTVPESGERCVYEEIQANVVVVLDYICIDDAFTQ 62

Query: 67  -APTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWI---DGHHERGGDVSLS 126
             PT+ V+VTSPYG  LY   N T+GQ AFTT+E+G  LAC  +     HH     V +S
Sbjct: 63  LGPTLDVRVTSPYGKELYKIANVTHGQAAFTTSESGTFLACLAMHHDQSHHSVNSSVIVS 122

Query: 127 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 186
           L+W+ GI AKDW+SVAKKEKIEGVELE+R+      AI  NILYL+ REA MR ++EKTN
Sbjct: 123 LDWKMGIRAKDWDSVAKKEKIEGVELEIRRSTEYASAIRANILYLRIREAYMREINEKTN 182

Query: 187 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
            RV    +MSLGV I+ S  Q+ YLKR+F KKKLI
Sbjct: 183 TRVNQLGLMSLGVAIVVSISQVLYLKRYFLKKKLI 217

BLAST of Cp4.1LG04g06630 vs. ExPASy Swiss-Prot
Match: Q9LQY3 (Transmembrane emp24 domain-containing protein p24delta9 OS=Arabidopsis thaliana OX=3702 GN=At1g26690 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 6.7e-39
Identity = 85/211 (40.28%), Postives = 138/211 (65.40%), Query Frame = 0

Query: 10  SLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYTH----A 69
           +L  IL FLA    + + ++ F L S  TKC+SE+I +N + +  Y V++ N  H    +
Sbjct: 7   NLCTILLFLAIS-SQVSQSLHFELQSGRTKCISEDIKSNSMTVGKYTVVNPNEAHPSPQS 66

Query: 70  PTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFW-IDGHHERGGDVSLSLEWR 129
             +S++VTS YGN  +H E+  +GQFAFT  E+G+++AC+  +D  H+    +S+  +WR
Sbjct: 67  HKISIRVTSSYGNTYHHAEDVESGQFAFTAVESGDYMACYTAVD--HKPEVTLSIDFDWR 126

Query: 130 TGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRVA 189
           TG+ +K W SVAKK ++E +E ++++L   V +IHE + YL+ERE EM+ ++  TN ++A
Sbjct: 127 TGVQSKSWSSVAKKSQVEVMEFDVKRLIETVNSIHEEMFYLREREEEMQNLNRATNSKMA 186

Query: 190 WFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
           W S +SL VC+  + +Q  +LK FF+KKK+I
Sbjct: 187 WLSFLSLFVCLGVAGMQFVHLKTFFEKKKVI 214

BLAST of Cp4.1LG04g06630 vs. NCBI nr
Match: XP_023529227.1 (transmembrane emp24 domain-containing protein p24delta4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427 bits (1099), Expect = 6.09e-151
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. NCBI nr
Match: KAG7022584.1 (Transmembrane emp24 domain-containing protein p24delta3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 426 bits (1095), Expect = 2.48e-150
Identity = 213/215 (99.07%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           MERR+MILPSLLLILSFLAADLVRPTHA+WFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MERRKMILPSLLLILSFLAADLVRPTHAIWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. NCBI nr
Match: XP_022927935.1 (transmembrane emp24 domain-containing protein p24delta4-like [Cucurbita moschata])

HSP 1 Score: 426 bits (1095), Expect = 2.48e-150
Identity = 213/215 (99.07%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           MERRRMILPSLLLILSFLAADLVRPTHA+WFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MERRRMILPSLLLILSFLAADLVRPTHAIWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVA+KEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVARKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. NCBI nr
Match: XP_022989723.1 (transmembrane emp24 domain-containing protein p24delta4-like [Cucurbita maxima])

HSP 1 Score: 424 bits (1091), Expect = 1.01e-149
Identity = 212/215 (98.60%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           ME+RRMILPSLLLILSFLAADLVRPTHA+WFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MEQRRMILPSLLLILSFLAADLVRPTHAIWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVA+KEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVARKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. NCBI nr
Match: KAG6588818.1 (Transmembrane emp24 domain-containing protein p24delta3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 398 bits (1023), Expect = 1.56e-138
Identity = 200/202 (99.01%), Postives = 201/202 (99.50%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           MERRRMILPSLLLILSFLAADLVR THA+WFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MERRRMILPSLLLILSFLAADLVRTTHAIWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQL 202
           GRVAWFSIMSLGVCILASTLQL
Sbjct: 181 GRVAWFSIMSLGVCILASTLQL 202

BLAST of Cp4.1LG04g06630 vs. ExPASy TrEMBL
Match: A0A6J1EQ97 (transmembrane emp24 domain-containing protein p24delta4-like OS=Cucurbita moschata OX=3662 GN=LOC111434689 PE=3 SV=1)

HSP 1 Score: 426 bits (1095), Expect = 1.20e-150
Identity = 213/215 (99.07%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           MERRRMILPSLLLILSFLAADLVRPTHA+WFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MERRRMILPSLLLILSFLAADLVRPTHAIWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVA+KEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVARKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. ExPASy TrEMBL
Match: A0A6J1JR63 (transmembrane emp24 domain-containing protein p24delta4-like OS=Cucurbita maxima OX=3661 GN=LOC111486723 PE=3 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 4.89e-150
Identity = 212/215 (98.60%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           ME+RRMILPSLLLILSFLAADLVRPTHA+WFNLPSTGTKCVSEEIHNNVVVLADYVVISE
Sbjct: 1   MEQRRMILPSLLLILSFLAADLVRPTHAIWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS
Sbjct: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGITAKDWESVA+KEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN
Sbjct: 121 LEWRTGITAKDWESVARKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. ExPASy TrEMBL
Match: A0A6J1HR23 (transmembrane emp24 domain-containing protein p24delta3-like OS=Cucurbita maxima OX=3661 GN=LOC111467003 PE=3 SV=1)

HSP 1 Score: 379 bits (972), Expect = 6.77e-132
Identity = 185/215 (86.05%), Postives = 203/215 (94.42%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           ME R+MILP LLL+L+FLA DLVRPTHA+W N+PSTGTKCVSEEI +NVVV+ADYVVISE
Sbjct: 1   MEPRKMILPCLLLVLTFLATDLVRPTHAIWLNIPSTGTKCVSEEIQSNVVVMADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTH PT+SVKVTSPYGNNL++KENQT+GQFAFTTAEAGNHLACFWIDGH++ GGD SL+
Sbjct: 61  NYTHTPTISVKVTSPYGNNLHYKENQTSGQFAFTTAEAGNHLACFWIDGHNQGGGDASLN 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGI+AKDWESVAKKEKIEGVELELRKLEG+VEAIH N+LYLK REA+MRIVSEKTN
Sbjct: 121 LEWRTGISAKDWESVAKKEKIEGVELELRKLEGLVEAIHGNLLYLKRREADMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILAS LQLWYL RFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASALQLWYLTRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. ExPASy TrEMBL
Match: A0A6J1HGJ9 (transmembrane emp24 domain-containing protein p24delta3-like OS=Cucurbita moschata OX=3662 GN=LOC111464101 PE=3 SV=1)

HSP 1 Score: 377 bits (967), Expect = 3.91e-131
Identity = 185/215 (86.05%), Postives = 203/215 (94.42%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           ME R+MILP LLLIL+FLA DLVRPT+A+W N+PSTGTKCVSEEI +NVVV+ADYVVISE
Sbjct: 1   MEPRKMILPCLLLILTFLATDLVRPTYAIWLNIPSTGTKCVSEEIQSNVVVMADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTH PT+SVKVTSPYGNNL++KENQT+GQFAFTTAEAGNHLACFWIDGH++ G D SL+
Sbjct: 61  NYTHTPTISVKVTSPYGNNLHYKENQTSGQFAFTTAEAGNHLACFWIDGHNQGGADASLN 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           LEWRTGI+AKDWESVAKKEKIEGVELELRKLEG+VEAIH N+LYLK REA+MRIVSEKTN
Sbjct: 121 LEWRTGISAKDWESVAKKEKIEGVELELRKLEGLVEAIHGNLLYLKRREADMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCILAS LQLWYLKRFFQKKKLI
Sbjct: 181 GRVAWFSIMSLGVCILASALQLWYLKRFFQKKKLI 215

BLAST of Cp4.1LG04g06630 vs. ExPASy TrEMBL
Match: A0A5D3CTD0 (Transmembrane emp24 domain-containing protein p24delta3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00250 PE=3 SV=1)

HSP 1 Score: 373 bits (958), Expect = 9.21e-130
Identity = 181/215 (84.19%), Postives = 198/215 (92.09%), Query Frame = 0

Query: 1   MERRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISE 60
           ME  RMILPS LLI +FL  DL+RPTHA+W NLPSTGTKCVSEEIH+NVVVLADYVVISE
Sbjct: 1   MEHWRMILPSFLLIFTFLVTDLLRPTHAIWLNLPSTGTKCVSEEIHSNVVVLADYVVISE 60

Query: 61  NYTHAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLS 120
           NYTH PTVSVKVTSPYGNNL+ KENQT GQFAFTTAEAGNHLACFW+DGHH+ GG+VSL+
Sbjct: 61  NYTHTPTVSVKVTSPYGNNLHQKENQTTGQFAFTTAEAGNHLACFWVDGHHQGGGEVSLN 120

Query: 121 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 180
           L+WRTGI+AKDW+SVAKKEKIEGV+LEL KLEG V+AIHEN++YLK REA+MRIVSEKTN
Sbjct: 121 LDWRTGISAKDWDSVAKKEKIEGVQLELLKLEGAVQAIHENLIYLKNREADMRIVSEKTN 180

Query: 181 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 215
           GRVAWFSIMSLGVCI AS LQLWYLKRFF KKKLI
Sbjct: 181 GRVAWFSIMSLGVCIFASALQLWYLKRFFHKKKLI 215

BLAST of Cp4.1LG04g06630 vs. TAIR 10
Match: AT1G09580.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-74
Identity = 138/213 (64.79%), Postives = 171/213 (80.28%), Query Frame = 0

Query: 3   RRRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENY 62
           + R   P+  L+L FL   ++    AVW ++P TGTKCVSEEI +NVVVLADY++ISE++
Sbjct: 6   KMRREFPTAFLLL-FLVPVMIPVGEAVWLDVPPTGTKCVSEEIQSNVVVLADYLIISEDH 65

Query: 63  THAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLE 122
              PT+SVKVTSPYGNNL++ EN T+GQFAFTT E+GN+LACFW D       +VS++++
Sbjct: 66  EVMPTISVKVTSPYGNNLHNMENVTHGQFAFTTQESGNYLACFWADEKSHGNKNVSINID 125

Query: 123 WRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGR 182
           WRTGI AKDW S+AKKEKIEGVELE+RKLEG VEAIHENILYL+ REA+MR +SEKTN R
Sbjct: 126 WRTGIAAKDWASIAKKEKIEGVELEIRKLEGAVEAIHENILYLRNREADMRTMSEKTNSR 185

Query: 183 VAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
           VAW+SIMSLGVCI  S  Q+ YLK++F+KKKLI
Sbjct: 186 VAWYSIMSLGVCIAVSGFQVLYLKQYFEKKKLI 217

BLAST of Cp4.1LG04g06630 vs. TAIR 10
Match: AT1G57620.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 269.2 bits (687), Expect = 2.8e-72
Identity = 133/212 (62.74%), Postives = 170/212 (80.19%), Query Frame = 0

Query: 4   RRMILPSLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYT 63
           ++ ++P+ +L LS L   L     AVW  +P TG+KCVSEEI +NV+VLADY+VISE ++
Sbjct: 2   KKKMIPTTIL-LSALIFSLSPICEAVWLTVPHTGSKCVSEEIQSNVIVLADYLVISEEHS 61

Query: 64  HAPTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLEW 123
             PTVSVKVT+PYG  L+H+EN TNGQFAFTT E+G +LACF  D       D S++++W
Sbjct: 62  IFPTVSVKVTAPYGTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNKDFSINIDW 121

Query: 124 RTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRV 183
           +TGI AKDW+S+A+KEKIEGVELE +KLEG VEAIHEN++YL+ REAEMRIVSEKTN RV
Sbjct: 122 KTGIAAKDWDSIARKEKIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRV 181

Query: 184 AWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
           AW+SIMSLG+CI+ S LQ+ YLK++F+KKKLI
Sbjct: 182 AWYSIMSLGICIVVSGLQILYLKQYFEKKKLI 212

BLAST of Cp4.1LG04g06630 vs. TAIR 10
Match: AT1G21900.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 256.9 bits (655), Expect = 1.4e-68
Identity = 132/207 (63.77%), Postives = 161/207 (77.78%), Query Frame = 0

Query: 11  LLLILSFLAADLVRPTHAVWFNLPST-GTKCVSEEIHNNVVVLADYVVISE-NYTHAPTV 70
           L ++L FL    V    A+W  +P+T GTKCVSEEI +NVVVLADY V+ E N  + P V
Sbjct: 13  LTVVLFFLT---VNYGEAIWLTIPTTGGTKCVSEEIQSNVVVLADYYVVDEHNPENTPAV 72

Query: 71  SVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWIDGHHERGGDVSLSLEWRTGIT 130
           S KVTSPYGNNL+H+EN T+GQFAFTT EAGN+LACFWID  H     ++L ++W+ GI 
Sbjct: 73  SSKVTSPYGNNLHHQENVTHGQFAFTTQEAGNYLACFWIDSSHHLANPITLGVDWKMGIA 132

Query: 131 AKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRVAWFSI 190
           AKDW+SVAKKEKIEGVEL+LR+LEG+V +I EN+ Y+K+REAEMR VSE TN RVAWFSI
Sbjct: 133 AKDWDSVAKKEKIEGVELQLRRLEGLVLSIRENLNYIKDREAEMREVSETTNSRVAWFSI 192

Query: 191 MSLGVCILASTLQLWYLKRFFQKKKLI 216
           MSLGVC++    Q+ YLKR+F KKKLI
Sbjct: 193 MSLGVCVVVVGSQILYLKRYFHKKKLI 216

BLAST of Cp4.1LG04g06630 vs. TAIR 10
Match: AT3G10780.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 196.4 bits (498), Expect = 2.3e-50
Identity = 111/215 (51.63%), Postives = 140/215 (65.12%), Query Frame = 0

Query: 7   ILPSLLLILSFLA--ADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYTH 66
           I P L + L +LA    L     A+W  +P +G +CV EEI  NVVV+ DY+ I + +T 
Sbjct: 3   ISPVLFIGLIYLAGGGSLFPGVEAIWLTVPESGERCVYEEIQANVVVVLDYICIDDAFTQ 62

Query: 67  -APTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFWI---DGHHERGGDVSLS 126
             PT+ V+VTSPYG  LY   N T+GQ AFTT+E+G  LAC  +     HH     V +S
Sbjct: 63  LGPTLDVRVTSPYGKELYKIANVTHGQAAFTTSESGTFLACLAMHHDQSHHSVNSSVIVS 122

Query: 127 LEWRTGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTN 186
           L+W+ GI AKDW+SVAKKEKIEGVELE+R+      AI  NILYL+ REA MR ++EKTN
Sbjct: 123 LDWKMGIRAKDWDSVAKKEKIEGVELEIRRSTEYASAIRANILYLRIREAYMREINEKTN 182

Query: 187 GRVAWFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
            RV    +MSLGV I+ S  Q+ YLKR+F KKKLI
Sbjct: 183 TRVNQLGLMSLGVAIVVSISQVLYLKRYFLKKKLI 217

BLAST of Cp4.1LG04g06630 vs. TAIR 10
Match: AT1G26690.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 162.2 bits (409), Expect = 4.8e-40
Identity = 85/211 (40.28%), Postives = 138/211 (65.40%), Query Frame = 0

Query: 10  SLLLILSFLAADLVRPTHAVWFNLPSTGTKCVSEEIHNNVVVLADYVVISENYTH----A 69
           +L  IL FLA    + + ++ F L S  TKC+SE+I +N + +  Y V++ N  H    +
Sbjct: 7   NLCTILLFLAIS-SQVSQSLHFELQSGRTKCISEDIKSNSMTVGKYTVVNPNEAHPSPQS 66

Query: 70  PTVSVKVTSPYGNNLYHKENQTNGQFAFTTAEAGNHLACFW-IDGHHERGGDVSLSLEWR 129
             +S++VTS YGN  +H E+  +GQFAFT  E+G+++AC+  +D  H+    +S+  +WR
Sbjct: 67  HKISIRVTSSYGNTYHHAEDVESGQFAFTAVESGDYMACYTAVD--HKPEVTLSIDFDWR 126

Query: 130 TGITAKDWESVAKKEKIEGVELELRKLEGVVEAIHENILYLKEREAEMRIVSEKTNGRVA 189
           TG+ +K W SVAKK ++E +E ++++L   V +IHE + YL+ERE EM+ ++  TN ++A
Sbjct: 127 TGVQSKSWSSVAKKSQVEVMEFDVKRLIETVNSIHEEMFYLREREEEMQNLNRATNSKMA 186

Query: 190 WFSIMSLGVCILASTLQLWYLKRFFQKKKLI 216
           W S +SL VC+  + +Q  +LK FF+KKK+I
Sbjct: 187 WLSFLSLFVCLGVAGMQFVHLKTFFEKKKVI 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6IDL41.9e-7364.79Transmembrane emp24 domain-containing protein p24delta3 OS=Arabidopsis thaliana ... [more]
Q9FVU03.9e-7162.74Transmembrane emp24 domain-containing protein p24delta4 OS=Arabidopsis thaliana ... [more]
Q8RWM62.0e-6763.77Transmembrane emp24 domain-containing protein p24delta5 OS=Arabidopsis thaliana ... [more]
F4J4Y03.2e-4951.63Transmembrane emp24 domain-containing protein p24delta6 OS=Arabidopsis thaliana ... [more]
Q9LQY36.7e-3940.28Transmembrane emp24 domain-containing protein p24delta9 OS=Arabidopsis thaliana ... [more]
Match NameE-valueIdentityDescription
XP_023529227.16.09e-151100.00transmembrane emp24 domain-containing protein p24delta4-like [Cucurbita pepo sub... [more]
KAG7022584.12.48e-15099.07Transmembrane emp24 domain-containing protein p24delta3 [Cucurbita argyrosperma ... [more]
XP_022927935.12.48e-15099.07transmembrane emp24 domain-containing protein p24delta4-like [Cucurbita moschata... [more]
XP_022989723.11.01e-14998.60transmembrane emp24 domain-containing protein p24delta4-like [Cucurbita maxima][more]
KAG6588818.11.56e-13899.01Transmembrane emp24 domain-containing protein p24delta3, partial [Cucurbita argy... [more]
Match NameE-valueIdentityDescription
A0A6J1EQ971.20e-15099.07transmembrane emp24 domain-containing protein p24delta4-like OS=Cucurbita moscha... [more]
A0A6J1JR634.89e-15098.60transmembrane emp24 domain-containing protein p24delta4-like OS=Cucurbita maxima... [more]
A0A6J1HR236.77e-13286.05transmembrane emp24 domain-containing protein p24delta3-like OS=Cucurbita maxima... [more]
A0A6J1HGJ93.91e-13186.05transmembrane emp24 domain-containing protein p24delta3-like OS=Cucurbita moscha... [more]
A0A5D3CTD09.21e-13084.19Transmembrane emp24 domain-containing protein p24delta3-like OS=Cucumis melo var... [more]
Match NameE-valueIdentityDescription
AT1G09580.11.3e-7464.79emp24/gp25L/p24 family/GOLD family protein [more]
AT1G57620.12.8e-7262.74emp24/gp25L/p24 family/GOLD family protein [more]
AT1G21900.11.4e-6863.77emp24/gp25L/p24 family/GOLD family protein [more]
AT3G10780.12.3e-5051.63emp24/gp25L/p24 family/GOLD family protein [more]
AT1G26690.14.8e-4040.28emp24/gp25L/p24 family/GOLD family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 141..161
NoneNo IPR availablePANTHERPTHR22811:SF127EMP24/GP25L/P24 FAMILY PROTEINcoord: 5..215
IPR009038GOLD domainSMARTSM01190EMP24_GP25L_2coord: 28..210
e-value: 1.9E-60
score: 216.8
IPR009038GOLD domainPFAMPF01105EMP24_GP25Lcoord: 29..210
e-value: 3.5E-50
score: 170.5
IPR009038GOLD domainPROSITEPS50866GOLDcoord: 38..150
score: 14.21346
IPR015720Transmembrane emp24 domain-containing proteinPANTHERPTHR22811TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEINcoord: 5..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g06630.1Cp4.1LG04g06630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process GO:0007030 Golgi organization
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0030134 COPII-coated ER to Golgi transport vesicle
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005793 endoplasmic reticulum-Golgi intermediate compartment
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane