Cp4.1LG04g06100 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g06100 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 7.9e-129
Identity = 279/754 (37.00%), Postives = 449/754 (59.55%), Query Frame = 0

Query: 40  LLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTSFGTIFYFFLVG 99
           +LKP  QP ++S+ILGG+VLG S L     F  TIFP R  + L+ + + G +++ FLVG
Sbjct: 57  ILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVG 116

Query: 100 VQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNT---VGTRTAKSLLIVSAAES 159
           V++DI +++   K++L I    ++LP ++ + +S ++  +   +G  T   +L +  A S
Sbjct: 117 VEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGT--YILFLGVALS 176

Query: 160 FINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGAVADSVISL 219
              FP++A +L+EL L+N+E GRI++++++V+ ++    L +   +A  +     S+  +
Sbjct: 177 VTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVM 236

Query: 220 STFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEGFVVALLLGVLITAFSSKAFGLHIYFG 279
            +  V     +F+VR   +WII+  P GE   E  +  +L GV+I+ F + A G H  FG
Sbjct: 237 ISSAVFIAVCVFVVRPGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFG 296

Query: 280 PLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIK-LKNFLGVSFVI 339
             + G+ IP G P+G  L+E+L+   S + +P+FF   GL  N+ +I+    +L +  VI
Sbjct: 297 AFVFGLVIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVI 356

Query: 340 YVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGRIDRGTFAV 399
           +++  GK +G ++VA  +  MP+R+ ++LGL++ ++G  E+ +  + + +  +D  TFA 
Sbjct: 357 FLACAGKVIGTVIVAFFH-GMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFAT 416

Query: 400 MCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGSDLRVLICIHDQEDVPNAIN 459
           M +  +V+  VITPI+  L+ P ++   YKRRT+   KP S+LRVL+C+H   +VP  IN
Sbjct: 417 MVLVALVMTGVITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIIN 476

Query: 460 LLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKD----RISTSCPSEHIVNAFKY 519
           LLEA +PT+RS + +Y+LHLVEL GRA++ LI HN +K        T   S+HI+NAF+ 
Sbjct: 477 LLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFEN 536

Query: 520 FGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISLSNYDM 579
           +   +   VA+ P T+I+  +TMH+DV SLA +K VS I+IPFHK+   +G +  +N   
Sbjct: 537 Y-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAY 596

Query: 580 KMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFVGARMA 639
           ++VN  +L+ +PCSVG++VDRG LN +    +N  S QVAV+F GG DDREA+    RMA
Sbjct: 597 RLVNQNLLENSPCSVGILVDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMA 656

Query: 640 GHPDINLTLIRLLKKGN-----------------VERDDVKETRLDNEAVLQFRKATTNN 699
            HP I LT++R +   +                  + D  K+ +LD++ +  FR      
Sbjct: 657 QHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEY 716

Query: 700 RRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGE 759
             ++ IE++V +G  TV+ +RSM +S DL IVGR       L  GL  W+E  ELGAIG+
Sbjct: 717 ESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGD 776

Query: 760 VLASSDFMGNAIILVVQQHTKLANEEQNKNNEDS 769
           +LASSDF     +LVVQQ+     +E + +  +S
Sbjct: 777 LLASSDFAATVSVLVVQQYVGSWAQEDDMDFPES 804

BLAST of Cp4.1LG04g06100 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 6.1e-105
Identity = 249/754 (33.02%), Postives = 422/754 (55.97%), Query Frame = 0

Query: 38  HQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGF--VFLDVVTSFGTIFYF 97
           H  L+ LG     S +L G++L  S L      R   F    +  +   +  +   + ++
Sbjct: 73  HFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAAR-RFFSTEDYKEIVFSLTAACSYMMFW 132

Query: 98  FLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTR----TAKSL--L 157
           FL+GV++D  +++   +K++ IG  SV+L  ++ S+     +  VGT+    T  SL  +
Sbjct: 133 FLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYV 192

Query: 158 IVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACF-------------- 217
           ++ + +   +FP+V +LL EL L NSE GR+A++S+++S   T+                
Sbjct: 193 VIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQ 252

Query: 218 -----LMIGTLVAPQNGAVADSVISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEG 277
                + IG ++A     +   ++ L  F+ ++   I++ R    +IIK  P+G P+K  
Sbjct: 253 TRLGSVFIGDVIAGNRPLMRAGIVVL--FVCIA---IYVFRPLMFYIIKQTPSGRPVKAI 312

Query: 278 FVVALLLGVLITAFSSKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVF 337
           ++  +++ V  +A  +      I+ GP ILG+++P GPP+GSA++++ +      F+P F
Sbjct: 313 YLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFF 372

Query: 338 FVKLGLVINVYSI-KLKNFLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMA 397
                  I++ ++   +   G+  ++  S + KF+   + A+ Y  MP+ D  +L L+M+
Sbjct: 373 IASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFY-GMPMEDCFALSLIMS 432

Query: 398 SQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTV 457
            +G FELG + L  + G +   TF V C+++ +  A+I PI+RYL+DP+R Y  Y++R +
Sbjct: 433 FKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNM 492

Query: 458 MDLKPGSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPH 517
             LKP S+LR+L CI+  +D+   INLLEA+ P+R S +  Y+LHL+EL+G+AN   I H
Sbjct: 493 QHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISH 552

Query: 518 NLKKDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSL 577
            L+  R   +  S +++ +F+ F ++   +V +  +T+++   TMH D+  LALN T SL
Sbjct: 553 KLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSL 612

Query: 578 ILIPFHKKFYSNGVISLSNYDM-KMVNNEILDKAPCSVGLVVDR---GVLNLS------R 637
           IL+PFH+ + ++G   +SN +M + +N  +LD APCSVG+ V R   G  N+S       
Sbjct: 613 ILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTIN 672

Query: 638 STATNLHSFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLLKKGNVERDD-VKETR 697
            T  NL S+ + ++FLGG DDREA+ +  RMA  P IN+T++RL+      R++ V +  
Sbjct: 673 GTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKM 732

Query: 698 LDNEAVLQFRKATTNNRRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVE 753
           LD+E +   +  T  +  +   E+ + D   T SLLRSM + FD+ IVGR +    +  E
Sbjct: 733 LDDELLRDVKSNTLVD--IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTE 792

BLAST of Cp4.1LG04g06100 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 2.0e-103
Identity = 243/777 (31.27%), Postives = 430/777 (55.34%), Query Frame = 0

Query: 36  LFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTSFGTIFYF 95
           L ++LLKPL Q +I +Q+L G++LG S       +     P+ G + L  +++ G   + 
Sbjct: 66  LLYRLLKPLKQGMISAQVLAGIILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHL 125

Query: 96  FLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNT--VGTRTAKSLLIVSA 155
           FL+G+++D SI++    K++ IGT S  LP  L +L    L NT  +       +  V +
Sbjct: 126 FLLGLRIDASIIRKAGSKAILIGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVIS 185

Query: 156 AESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGAVAD-- 215
             +  +FP+  ++L+EL+++NS+ GR+A   S+V   ++    ++  +   ++G +A   
Sbjct: 186 LNAMTSFPVTTTVLAELNILNSDLGRLATNCSIVCEAFSWIVALVFRMFL-RDGTLASVW 245

Query: 216 SVISLSTFIVVSGTIIFIVRLATSWIIKNNPAG-EPLKEGFVVALLLGVLITAFSSKAFG 275
           S + ++  I+V   I F+ R A  W+ +      +   E     +++ +L  + +S+  G
Sbjct: 246 SFVWVTALILV---IFFVCRPAIIWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLG 305

Query: 276 LHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNFLG 335
           +H  FG   LGVS+P GPP+G+ L  +L+   + + +P F    GL  N + I   +   
Sbjct: 306 VHAAFGAFWLGVSLPDGPPLGTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKI 365

Query: 336 VSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGRIDR 395
           +  VI ++   KFLG    A  YC + + DA SL L+M  QG  E+    + + E  ++ 
Sbjct: 366 IEAVILITYGCKFLGT-AAASAYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNT 425

Query: 396 GTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGS-DLRVLICIHDQED 455
             F ++ I L+++  +   ++  L+DP++RY    +RT++D +  +   R+L+C+++ E+
Sbjct: 426 ECFNLLIITLLLVTGISRFLVVCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVEN 485

Query: 456 VPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIVNAF 515
           VP+ +NLLEA  P+R S + V+ LHLVEL GRA++ L+PH+        +  S HIVN F
Sbjct: 486 VPSMVNLLEASYPSRFSPISVFTLHLVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGF 545

Query: 516 KYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISLSNY 575
           + F + N+ T+    FT+    ++++DD+ +LAL+K  +LI+IPFHK++  +G +   N 
Sbjct: 546 QRFEQQNQGTLMAQHFTAAAPFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNP 605

Query: 576 DMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFVGAR 635
            ++ +N  +L+KAPCSVG+ +DRG     RS   +     VAV+F+ G DD EA+    R
Sbjct: 606 SIRNINLNVLEKAPCSVGIFIDRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMR 665

Query: 636 MAGHPDINLTLIRLLKKGNVERD---DVKETRLDNEAVLQFRKATTNNRRVMCIEEVVMD 695
           +A HP++++T+I    K +++++   DV+    ++  +  F+    +  ++   EE+V D
Sbjct: 666 IAEHPEVSVTMIHFRHKSSLQQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRD 725

Query: 696 GTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNAI 755
           G  T  ++ S+G+SFDLV+VGR H     ++ GL  W+E  ELG IG++ ASSDF  + +
Sbjct: 726 GVETTQVISSLGDSFDLVVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVL 785

Query: 756 ILVVQQHTKLANEEQNKNNEDSSQVRIPRSKMGSIVMEPGDNSIFNNFSSICVHVDR 804
           ++  Q+   LA +   K      +V  PR       + P   S+   F+S+ +H +R
Sbjct: 786 VIHQQEGDSLAMDNSYKLPASPHRVGDPR-------VHP-RFSVEEGFTSVDLHSNR 829

BLAST of Cp4.1LG04g06100 vs. ExPASy Swiss-Prot
Match: Q9FYC1 (Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 9.4e-98
Identity = 238/757 (31.44%), Postives = 421/757 (55.61%), Query Frame = 0

Query: 37  FHQLLKPLGQPLIVSQILGGLVLGSSGLSH---------MKTFRDTIFPLRGFVFLDVVT 96
           FH  L+ LG     S +L G++L  S L            + +++T+F L G        
Sbjct: 71  FHFFLRRLGMIRFTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLFGLVG-------- 130

Query: 97  SFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAK- 156
           +   + ++FL+GV++D+S++++  +K++ IG  SV+L + + +L    ++  VGT+  + 
Sbjct: 131 ACSYMMFWFLMGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEP 190

Query: 157 -----SLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVS----TIWTACFLM 216
                 ++ +   +   +FP++ +LL EL L NSE GR+A++S+++S    +I +A  + 
Sbjct: 191 VMSFFEIIFIYLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVF 250

Query: 217 IGTLVAPQNGA----VADSVIS------LSTFIVVSGTIIFIVRLATSWIIKNNPAGEPL 276
           +  L   ++      + D ++         T ++     I+I R    +IIK  P+G P+
Sbjct: 251 LKELKDDKSRLGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPV 310

Query: 277 KEGFVVALLLGVLITAFSSKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFM 336
           K+ ++ A+++ V  +A  +      I+ GP ILG+++P GPP+GSA++++ + +    F+
Sbjct: 311 KKFYIYAIIILVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFL 370

Query: 337 PVFFVKLGLVINVYSIK-LKNFLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGL 396
           P F       I+   ++   +   +  ++ VS + KF    L A +Y  MP +D ++L L
Sbjct: 371 PFFVATSAEEIDTSILQSWIDLKSIVILVSVSFIVKFALTTLPAFLY-GMPAKDCIALSL 430

Query: 397 VMASQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKR 456
           +M+ +G FE G +    + G I   TF V+ +++++  AVI P+++ ++DP+R Y  Y++
Sbjct: 431 IMSFKGIFEFGAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEK 490

Query: 457 RTVMDLKPGSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQL 516
           R ++ +KP S+LR+L CI+  +D+   INLLEA  P+R + +  Y+LHL+EL+G+AN  L
Sbjct: 491 RNMLHMKPNSELRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVL 550

Query: 517 IPHNLK-KDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNK 576
           I H L+ +   + S  SE++V +F+ F  +   +V +  +T+++    MH D+  LALN 
Sbjct: 551 ISHRLQTRKSENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMMHGDICMLALNN 610

Query: 577 TVSLILIPFHKKFYSNGVISLSNYDM-KMVNNEILDKAPCSVGLVVDRGV--LNLSRSTA 636
           T SLI++PFH+ + ++G   +S+  M + +N  +LD +PCSVG+ V R        + TA
Sbjct: 611 TTSLIILPFHQTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGRRTIKETA 670

Query: 637 TNLHSFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLL---KKGNVERDDVKETRL 696
            N  S+QV ++FLGG DDREA+ +  RMA    I +T++ L+   ++ N   D   +  L
Sbjct: 671 ANFSSYQVCMLFLGGKDDREALSLAKRMARDSRITITVVSLISSEQRANQATD--WDRML 730

Query: 697 DNEAVLQFRKATTNNRRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEG 756
           D E +   +        ++  EEVV D   T  LL+S+ N +DL IVGR      +  EG
Sbjct: 731 DLELLRDVKSNVLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVFTEG 790

BLAST of Cp4.1LG04g06100 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 1.0e-96
Identity = 237/755 (31.39%), Postives = 404/755 (53.51%), Query Frame = 0

Query: 36  LFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTSFGTIFYF 95
           L  ++L+PL Q +I +Q+L G+VLG S L H   + +   P  G + +  +++ G + + 
Sbjct: 68  LIFRVLQPLKQGMISAQVLTGVVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHL 127

Query: 96  FLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAKSLLIVSA-- 155
           FL+G+++D SI++    K++ IGT S   P  L +L    +  T+G  +       SA  
Sbjct: 128 FLLGLKIDGSIIRKAGSKAILIGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAIS 187

Query: 156 AESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGAVADSV 215
             S  +FP+  ++L+EL+++NSE GR+A   SMV  + +    +   L          S+
Sbjct: 188 LSSMTSFPVTTTVLAELNILNSELGRLATHCSMVCEVCSWFVALAFNLYT--RDRTMTSL 247

Query: 216 ISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKE---GFVVALLLGVLITAFSSKAFG 275
            +LS  I +   I F+ R    W+ +        K+    F V LLL   I + S +A G
Sbjct: 248 YALSMIIGLLLVIYFVFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLS--IASLSGEAMG 307

Query: 276 LHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLK---N 335
           +H  FG   LGVS+P GPP+G+ L  +L+   S +F+P F    GL  N + I      +
Sbjct: 308 VHAAFGAFWLGVSLPDGPPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHH 367

Query: 336 FLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGR 395
            + +  ++ ++   KFLG    A  YC+  + DA+ L  +M  QG  E+    + +    
Sbjct: 368 VVMIEIILLITYGCKFLGT-AAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQV 427

Query: 396 IDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGS-DLRVLICIHD 455
           +D   F ++ I ++ +  +   ++ YL+DP++RY    +RT+++ +  +  LR+L+ +++
Sbjct: 428 VDTECFNLVIITILFVTGISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYN 487

Query: 456 QEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIV 515
            E+VP+ +NLLEA  PTR + +  + LHLVEL GRA++ L PH+        +  S HIV
Sbjct: 488 VENVPSMVNLLEATYPTRFNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIV 547

Query: 516 NAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISL 575
           NAF+ F +  +  +    FT+    +++++D+ +LAL+K  +LI+IPFHK++  +G +  
Sbjct: 548 NAFQRFEQKYQGALMAQHFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQ 607

Query: 576 SNYDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFV 635
            N  ++ +N  +LD APCSV + +DRG     RS         VA++F+GG DD EA+ +
Sbjct: 608 VNGPIRTINLNVLDAAPCSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALAL 667

Query: 636 GARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMCIEEVVMD 695
             RMA  PD+N+T+I    K  ++ +D  +   +   +  F+    N  ++  +EE+V D
Sbjct: 668 CMRMAEKPDLNVTMIHFRHKSALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRD 727

Query: 696 GTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNAI 755
           G  T  ++ S+G+++D+V+VGR H     ++ GL  W+E  ELG IG++L S DF  + +
Sbjct: 728 GVETTQVISSLGDAYDMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVL 787

Query: 756 ILVVQQ---------HTKLANEEQNKNNEDSSQVR 773
           ++  QQ           KL N E  K  +   Q R
Sbjct: 788 VVHQQQGDDLLAMDDSYKLPNVEHQKIGDTGIQQR 816

BLAST of Cp4.1LG04g06100 vs. NCBI nr
Match: XP_023531606.1 (cation/H(+) antiporter 15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1513 bits (3918), Expect = 0.0
Identity = 788/788 (100.00%), Postives = 788/788 (100.00%), Query Frame = 0

Query: 778  MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 837
            MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL
Sbjct: 1    MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 838  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 897
            FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL
Sbjct: 61   FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 898  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 957
            IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF
Sbjct: 121  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 958  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 1017
            IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY
Sbjct: 181  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 1018 GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 1077
            GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI
Sbjct: 241  GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 300

Query: 1078 LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 1137
            LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA
Sbjct: 301  LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 360

Query: 1138 AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC 1197
            AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC
Sbjct: 361  AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC 420

Query: 1198 VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 1257
            VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA
Sbjct: 421  VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 480

Query: 1258 LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD 1317
            LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD
Sbjct: 481  LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD 540

Query: 1318 VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 1377
            VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI
Sbjct: 541  VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 600

Query: 1378 LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV 1437
            LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV
Sbjct: 601  LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV 660

Query: 1438 TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE 1497
            TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE
Sbjct: 661  TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE 720

Query: 1498 NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN 1557
            NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN
Sbjct: 721  NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN 780

Query: 1558 EDPETSKR 1565
            EDPETSKR
Sbjct: 781  EDPETSKR 788

BLAST of Cp4.1LG04g06100 vs. NCBI nr
Match: XP_022928208.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1496 bits (3874), Expect = 0.0
Identity = 776/788 (98.48%), Postives = 783/788 (99.37%), Query Frame = 0

Query: 778  MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 837
            MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL
Sbjct: 1    MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 838  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 897
            FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL
Sbjct: 61   FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 898  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 957
            IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF
Sbjct: 121  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 958  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 1017
            IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY
Sbjct: 181  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 1018 GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 1077
            GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI
Sbjct: 241  GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 300

Query: 1078 LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 1137
            LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA
Sbjct: 301  LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 360

Query: 1138 AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC 1197
            AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLM KDKVLDEETFVVMC+C
Sbjct: 361  AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKKDKVLDEETFVVMCIC 420

Query: 1198 VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 1257
            VMLVAAVITPILRYLFDPSRRFV EKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA
Sbjct: 421  VMLVAAVITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 480

Query: 1258 LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD 1317
            LKP HR+HLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSY SESIVNAFNYFGQSNSD
Sbjct: 481  LKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNSD 540

Query: 1318 VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 1377
            VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI
Sbjct: 541  VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 600

Query: 1378 LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV 1437
            LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPY+NV
Sbjct: 601  LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINV 660

Query: 1438 TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE 1497
            TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILR+L+
Sbjct: 661  TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTLD 720

Query: 1498 NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN 1557
            NNFDLI+VGRRHSPRSPLVQGLLLWNEQTELGS+GEVLASSDFIGNATI VVQQHRKVVN
Sbjct: 721  NNFDLILVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVN 780

Query: 1558 EDPETSKR 1565
            EDPETSKR
Sbjct: 781  EDPETSKR 788

BLAST of Cp4.1LG04g06100 vs. NCBI nr
Match: KAG6588887.1 (Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1492 bits (3862), Expect = 0.0
Identity = 773/788 (98.10%), Postives = 781/788 (99.11%), Query Frame = 0

Query: 778  MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 837
            MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL
Sbjct: 1    MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 838  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 897
            FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL
Sbjct: 61   FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 898  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 957
            IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF
Sbjct: 121  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 958  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 1017
            IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY
Sbjct: 181  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 1018 GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 1077
            GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMH+YFGPI
Sbjct: 241  GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHVYFGPI 300

Query: 1078 LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 1137
            LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA
Sbjct: 301  LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 360

Query: 1138 AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC 1197
            AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLM KDKVLDEETFVVMC+C
Sbjct: 361  AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKKDKVLDEETFVVMCIC 420

Query: 1198 VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 1257
            VMLVAA ITPILRYLFDPSRRFV EKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA
Sbjct: 421  VMLVAAFITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 480

Query: 1258 LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD 1317
            LKP HR+HLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSY SESIVNAFNYFGQSNSD
Sbjct: 481  LKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNSD 540

Query: 1318 VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 1377
            VVTIHP TAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI
Sbjct: 541  VVTIHPITAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 600

Query: 1378 LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV 1437
            LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPY+NV
Sbjct: 601  LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINV 660

Query: 1438 TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE 1497
            TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILR+L+
Sbjct: 661  TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTLD 720

Query: 1498 NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN 1557
            NNFDLI+VGRRHSPRSPLVQGLLLWNEQTELGS+GEVLASSDFIGNATI VVQQHRKVVN
Sbjct: 721  NNFDLILVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVN 780

Query: 1558 EDPETSKR 1565
            EDPETSKR
Sbjct: 781  EDPETSKR 788

BLAST of Cp4.1LG04g06100 vs. NCBI nr
Match: KAG7022651.1 (Cation/H(+) antiporter 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1489 bits (3855), Expect = 0.0
Identity = 771/788 (97.84%), Postives = 781/788 (99.11%), Query Frame = 0

Query: 778  MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 837
            MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL
Sbjct: 1    MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 838  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 897
            FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL
Sbjct: 61   FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 898  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 957
            IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF
Sbjct: 121  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 958  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 1017
            IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY
Sbjct: 181  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 1018 GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 1077
            GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMH+YFGPI
Sbjct: 241  GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHVYFGPI 300

Query: 1078 LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 1137
            LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA
Sbjct: 301  LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 360

Query: 1138 AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC 1197
            AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLM +DKVLDEETFVVMC+C
Sbjct: 361  AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKEDKVLDEETFVVMCIC 420

Query: 1198 VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 1257
            VMLVAA ITPILRYLFDPSRRFV EKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA
Sbjct: 421  VMLVAAFITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 480

Query: 1258 LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD 1317
            LKP HR+HLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSY SESIVNAFNYFGQSNSD
Sbjct: 481  LKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNSD 540

Query: 1318 VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 1377
            VVTIHP TAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI
Sbjct: 541  VVTIHPITAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 600

Query: 1378 LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV 1437
            LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPY+NV
Sbjct: 601  LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINV 660

Query: 1438 TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE 1497
            TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILR+L+
Sbjct: 661  TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTLD 720

Query: 1498 NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN 1557
            NNFDLI+VG+RHSPRSPLVQGLLLWNEQTELGS+GEVLASSDFIGNATI VVQQHRKVVN
Sbjct: 721  NNFDLILVGKRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVN 780

Query: 1558 EDPETSKR 1565
            EDPETSKR
Sbjct: 781  EDPETSKR 788

BLAST of Cp4.1LG04g06100 vs. NCBI nr
Match: XP_022989578.1 (cation/H(+) antiporter 15-like [Cucurbita maxima])

HSP 1 Score: 1465 bits (3793), Expect = 0.0
Identity = 759/783 (96.93%), Postives = 773/783 (98.72%), Query Frame = 0

Query: 783  MEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK 842
            ME  D+SIFNNFSSICVHVDRIHS+SVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK
Sbjct: 5    MESDDSSIFNNFSSICVHVDRIHSSSVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK 64

Query: 843  RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFLIGVQT 902
            RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAIS+LGQVFYFFLIGVQT
Sbjct: 65   RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISSLGQVFYFFLIGVQT 124

Query: 903  DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECFIHFAM 962
            DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLF+VGNAE FIHFAM
Sbjct: 125  DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFLVGNAESFIHFAM 184

Query: 963  VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVYGFIVL 1022
            VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVYGFIVL
Sbjct: 185  VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVYGFIVL 244

Query: 1023 AVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGIT 1082
            AVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGIT
Sbjct: 245  AVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGIT 304

Query: 1083 IPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVAAFGKF 1142
            IPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGL+FSIHTIKLLNFLCISFIIIVAAFGKF
Sbjct: 305  IPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLVFSIHTIKLLNFLCISFIIIVAAFGKF 364

Query: 1143 LGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLCVMLVA 1202
            LGALMMSIYYKLPTRDGVALGLILNSQGALEIGI+KLM KDKVLDEETFVVMC+CVM+VA
Sbjct: 365  LGALMMSIYYKLPTRDGVALGLILNSQGALEIGIYKLMKKDKVLDEETFVVMCICVMVVA 424

Query: 1203 AVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPMH 1262
            AVITPI+RYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPMH
Sbjct: 425  AVITPIIRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPMH 484

Query: 1263 RNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSDVVTIH 1322
            RNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRS  SESIVNAF YFGQSNSDVVTIH
Sbjct: 485  RNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSCVSESIVNAFKYFGQSNSDVVTIH 544

Query: 1323 PFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHILDKAP 1382
            PFTA+SPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKM NQHILDKAP
Sbjct: 545  PFTAISPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMFNQHILDKAP 604

Query: 1383 CSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNVTLIRL 1442
            CSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPY+NVTLIRL
Sbjct: 605  CSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINVTLIRL 664

Query: 1443 LEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLENNFDL 1502
            LEDTNSPSD GKEMKLNNEAVSKYHRL+ENNHRVRY+EEVAKDCTGTVAILRSLENNFDL
Sbjct: 665  LEDTNSPSDDGKEMKLNNEAVSKYHRLLENNHRVRYMEEVAKDCTGTVAILRSLENNFDL 724

Query: 1503 IMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVNEDPET 1562
            IMVGRRHSPRSPLVQGLLLWNEQTELGS+GEVLASSDFIGNATI VVQQHRKVVNEDPET
Sbjct: 725  IMVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVNEDPET 784

Query: 1563 SKR 1565
            SKR
Sbjct: 785  SKR 787

BLAST of Cp4.1LG04g06100 vs. ExPASy TrEMBL
Match: A0A6J1EN94 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435106 PE=4 SV=1)

HSP 1 Score: 1496 bits (3874), Expect = 0.0
Identity = 776/788 (98.48%), Postives = 783/788 (99.37%), Query Frame = 0

Query: 778  MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 837
            MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL
Sbjct: 1    MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 838  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 897
            FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL
Sbjct: 61   FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 898  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 957
            IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF
Sbjct: 121  IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 958  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 1017
            IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY
Sbjct: 181  IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 1018 GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 1077
            GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI
Sbjct: 241  GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPI 300

Query: 1078 LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 1137
            LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA
Sbjct: 301  LLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVA 360

Query: 1138 AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLC 1197
            AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLM KDKVLDEETFVVMC+C
Sbjct: 361  AFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKKDKVLDEETFVVMCIC 420

Query: 1198 VMLVAAVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 1257
            VMLVAAVITPILRYLFDPSRRFV EKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA
Sbjct: 421  VMLVAAVITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEA 480

Query: 1258 LKPMHRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSD 1317
            LKP HR+HLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSY SESIVNAFNYFGQSNSD
Sbjct: 481  LKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNSD 540

Query: 1318 VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 1377
            VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI
Sbjct: 541  VVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHI 600

Query: 1378 LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNV 1437
            LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPY+NV
Sbjct: 601  LDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINV 660

Query: 1438 TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLE 1497
            TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILR+L+
Sbjct: 661  TLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTLD 720

Query: 1498 NNFDLIMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVN 1557
            NNFDLI+VGRRHSPRSPLVQGLLLWNEQTELGS+GEVLASSDFIGNATI VVQQHRKVVN
Sbjct: 721  NNFDLILVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVN 780

Query: 1558 EDPETSKR 1565
            EDPETSKR
Sbjct: 781  EDPETSKR 788

BLAST of Cp4.1LG04g06100 vs. ExPASy TrEMBL
Match: A0A6J1JQQ2 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111486629 PE=4 SV=1)

HSP 1 Score: 1465 bits (3793), Expect = 0.0
Identity = 759/783 (96.93%), Postives = 773/783 (98.72%), Query Frame = 0

Query: 783  MEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK 842
            ME  D+SIFNNFSSICVHVDRIHS+SVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK
Sbjct: 5    MESDDSSIFNNFSSICVHVDRIHSSSVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK 64

Query: 843  RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFLIGVQT 902
            RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAIS+LGQVFYFFLIGVQT
Sbjct: 65   RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISSLGQVFYFFLIGVQT 124

Query: 903  DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECFIHFAM 962
            DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLF+VGNAE FIHFAM
Sbjct: 125  DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFLVGNAESFIHFAM 184

Query: 963  VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVYGFIVL 1022
            VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVYGFIVL
Sbjct: 185  VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVYGFIVL 244

Query: 1023 AVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGIT 1082
            AVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGIT
Sbjct: 245  AVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGIT 304

Query: 1083 IPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIVAAFGKF 1142
            IPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGL+FSIHTIKLLNFLCISFIIIVAAFGKF
Sbjct: 305  IPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLVFSIHTIKLLNFLCISFIIIVAAFGKF 364

Query: 1143 LGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMTKDKVLDEETFVVMCLCVMLVA 1202
            LGALMMSIYYKLPTRDGVALGLILNSQGALEIGI+KLM KDKVLDEETFVVMC+CVM+VA
Sbjct: 365  LGALMMSIYYKLPTRDGVALGLILNSQGALEIGIYKLMKKDKVLDEETFVVMCICVMVVA 424

Query: 1203 AVITPILRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPMH 1262
            AVITPI+RYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPMH
Sbjct: 425  AVITPIIRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPMH 484

Query: 1263 RNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYASESIVNAFNYFGQSNSDVVTIH 1322
            RNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRS  SESIVNAF YFGQSNSDVVTIH
Sbjct: 485  RNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSCVSESIVNAFKYFGQSNSDVVTIH 544

Query: 1323 PFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQHILDKAP 1382
            PFTA+SPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKM NQHILDKAP
Sbjct: 545  PFTAISPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMFNQHILDKAP 604

Query: 1383 CSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYVNVTLIRL 1442
            CSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPY+NVTLIRL
Sbjct: 605  CSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINVTLIRL 664

Query: 1443 LEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRSLENNFDL 1502
            LEDTNSPSD GKEMKLNNEAVSKYHRL+ENNHRVRY+EEVAKDCTGTVAILRSLENNFDL
Sbjct: 665  LEDTNSPSDDGKEMKLNNEAVSKYHRLLENNHRVRYMEEVAKDCTGTVAILRSLENNFDL 724

Query: 1503 IMVGRRHSPRSPLVQGLLLWNEQTELGSLGEVLASSDFIGNATIVVVQQHRKVVNEDPET 1562
            IMVGRRHSPRSPLVQGLLLWNEQTELGS+GEVLASSDFIGNATI VVQQHRKVVNEDPET
Sbjct: 725  IMVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVNEDPET 784

Query: 1563 SKR 1565
            SKR
Sbjct: 785  SKR 787

BLAST of Cp4.1LG04g06100 vs. ExPASy TrEMBL
Match: A0A6J1EQV6 (cation/H(+) antiporter 15-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435058 PE=4 SV=1)

HSP 1 Score: 1371 bits (3548), Expect = 0.0
Identity = 725/800 (90.62%), Postives = 744/800 (93.00%), Query Frame = 0

Query: 13  MSSILMEPDDLAVF--------------------------------------------FG 72
           MSSILMEPDDLAVF                                            FG
Sbjct: 1   MSSILMEPDDLAVFVGGSASRTSMNFTTLCTSAHRIHSAGVFSGVNPLEFSVPLLLLQFG 60

Query: 73  ICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTS 132
           ICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTS
Sbjct: 61  ICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTS 120

Query: 133 FGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAKSL 192
           FGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAKSL
Sbjct: 121 FGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAKSL 180

Query: 193 LIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGA 252
           LIVSAAESFINFPMVASLLSELHLMNSEFGRIAL+SSMVST+WT CF+MIG LVAPQNGA
Sbjct: 181 LIVSAAESFINFPMVASLLSELHLMNSEFGRIALSSSMVSTVWTFCFVMIGALVAPQNGA 240

Query: 253 VADSVISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEGFVVALLLGVLITAFSSKA 312
           VA+SVISLSTFI+VSGTIIF+VR A SWIIKN+P GEPLKEGFVVA+L+GVLITAFSSKA
Sbjct: 241 VAESVISLSTFIMVSGTIIFVVRQAMSWIIKNSPTGEPLKEGFVVAMLIGVLITAFSSKA 300

Query: 313 FGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNF 372
           FGLHIYFGPLILG+SIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNF
Sbjct: 301 FGLHIYFGPLILGISIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNF 360

Query: 373 LGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGRI 432
           LGVSFVIYVSALGKFLGAL+VAVV+CRMP+RDAVSLGLVMASQGAFELGMFKLMRKEGRI
Sbjct: 361 LGVSFVIYVSALGKFLGALVVAVVHCRMPVRDAVSLGLVMASQGAFELGMFKLMRKEGRI 420

Query: 433 DRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGSDLRVLICIHDQE 492
           DRGTFAVMCIFLMVL+AVITPIIRYLFDP+RRYTVYKRRTVMDLK GSDLRVLICIHDQE
Sbjct: 421 DRGTFAVMCIFLMVLMAVITPIIRYLFDPSRRYTVYKRRTVMDLKSGSDLRVLICIHDQE 480

Query: 493 DVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIVNA 552
           DVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIVNA
Sbjct: 481 DVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIVNA 540

Query: 553 FKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISLSN 612
           FKYFGRNNRQTVAIFPFTSITSSTTMHDDVY LALNK+VSLIL+PFHKKFYSNGVISLSN
Sbjct: 541 FKYFGRNNRQTVAIFPFTSITSSTTMHDDVYLLALNKSVSLILVPFHKKFYSNGVISLSN 600

Query: 613 YDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFVGA 672
           Y+MKMVNNEILDKAPCSVGLVVDRGVLNLSRS ATNLHSFQVAVVFLGGADDREAMFVGA
Sbjct: 601 YNMKMVNNEILDKAPCSVGLVVDRGVLNLSRSAATNLHSFQVAVVFLGGADDREAMFVGA 660

Query: 673 RMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMCIEEVVMDGT 732
           RMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVM IEEVVMDGT
Sbjct: 661 RMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMYIEEVVMDGT 720

Query: 733 GTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNAIIL 768
           GTVSLLRSMGNSF LVIVGRRHSCC  LV+GLVLWNEQTELGAIGEVLASSDFMGNAIIL
Sbjct: 721 GTVSLLRSMGNSFSLVIVGRRHSCCSTLVQGLVLWNEQTELGAIGEVLASSDFMGNAIIL 780

BLAST of Cp4.1LG04g06100 vs. ExPASy TrEMBL
Match: A0A6J1JMR6 (cation/H(+) antiporter 15-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486628 PE=4 SV=1)

HSP 1 Score: 1353 bits (3501), Expect = 0.0
Identity = 721/800 (90.12%), Postives = 737/800 (92.12%), Query Frame = 0

Query: 13  MSSILMEPDDLAVF--------------------------------------------FG 72
           MSS+LMEPDDLA F                                            FG
Sbjct: 1   MSSVLMEPDDLAAFVGGRASRTSMNFTTICTSAHRIHSTGVFSGVNPLEFSVPLLLLQFG 60

Query: 73  ICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTS 132
           ICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTS
Sbjct: 61  ICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTS 120

Query: 133 FGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAKSL 192
           FGTIFYFFLVGVQVDISIMK IDKKSLGIGTFSVILPLILTSLYSTA MN+V T+TAKSL
Sbjct: 121 FGTIFYFFLVGVQVDISIMKKIDKKSLGIGTFSVILPLILTSLYSTASMNSVDTKTAKSL 180

Query: 193 LIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGA 252
           LIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIG LVAPQNGA
Sbjct: 181 LIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGALVAPQNGA 240

Query: 253 VADSVISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEGFVVALLLGVLITAFSSKA 312
           VA+S+ISLS FIVVSGTIIFIVR ATSWII NNP GEPLKEGFVVALLLGVLITAFSSKA
Sbjct: 241 VAESMISLSIFIVVSGTIIFIVRQATSWIIMNNPTGEPLKEGFVVALLLGVLITAFSSKA 300

Query: 313 FGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNF 372
           FGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNF
Sbjct: 301 FGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNF 360

Query: 373 LGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGRI 432
           LGVSFVIYVSALGKFLGAL+VAVVYCRMP+RDAVSLGLVMASQGAFELGMFKLMRKEGRI
Sbjct: 361 LGVSFVIYVSALGKFLGALVVAVVYCRMPVRDAVSLGLVMASQGAFELGMFKLMRKEGRI 420

Query: 433 DRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGSDLRVLICIHDQE 492
           DRGTFAVMCIFLMVLVAVITPIIRYLFDP+RRYTVYKRRTVMDLKPGSDLRVLICIHDQE
Sbjct: 421 DRGTFAVMCIFLMVLVAVITPIIRYLFDPSRRYTVYKRRTVMDLKPGSDLRVLICIHDQE 480

Query: 493 DVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIVNA 552
           DVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTS PS+HIVNA
Sbjct: 481 DVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSRPSKHIVNA 540

Query: 553 FKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISLSN 612
           F YFGRNNRQTVAIFPFTSITSSTTMHDDVYSLAL KTVSLILIPFHKKFYSNGVISLSN
Sbjct: 541 FTYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALKKTVSLILIPFHKKFYSNGVISLSN 600

Query: 613 YDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFVGA 672
           YDMKMVNNEILDKAPCSVGLVVDRGVLNLSRS ATNLHSFQVAVVFLGGADDREAMFVGA
Sbjct: 601 YDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSAATNLHSFQVAVVFLGGADDREAMFVGA 660

Query: 673 RMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMCIEEVVMDGT 732
           RMAGHP+INLTLIRLLKK NV RDDVKETRLD+EAVLQFRKATTNN RVM IEEVVMDGT
Sbjct: 661 RMAGHPNINLTLIRLLKKVNVVRDDVKETRLDSEAVLQFRKATTNNHRVMYIEEVVMDGT 720

Query: 733 GTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNAIIL 768
           GTVS+LRSMGNSF+LVIVGRRHSCC MLV+GLVLWNEQTELGAIGEVLASSDFMGNAIIL
Sbjct: 721 GTVSVLRSMGNSFNLVIVGRRHSCCSMLVQGLVLWNEQTELGAIGEVLASSDFMGNAIIL 780

BLAST of Cp4.1LG04g06100 vs. ExPASy TrEMBL
Match: A0A6J1EJH8 (cation/H(+) antiporter 15-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435058 PE=4 SV=1)

HSP 1 Score: 1289 bits (3336), Expect = 0.0
Identity = 671/702 (95.58%), Postives = 690/702 (98.29%), Query Frame = 0

Query: 67  MKTFRDTIFPLRGFVFLDVVTSFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPL 126
           MKTFRDTIFPLRGFVFLDVVTSFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPL
Sbjct: 1   MKTFRDTIFPLRGFVFLDVVTSFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPL 60

Query: 127 ILTSLYSTALMNTVGTRTAKSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSM 186
           ILTSLYSTALMNTVGTRTAKSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIAL+SSM
Sbjct: 61  ILTSLYSTALMNTVGTRTAKSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALSSSM 120

Query: 187 VSTIWTACFLMIGTLVAPQNGAVADSVISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEP 246
           VST+WT CF+MIG LVAPQNGAVA+SVISLSTFI+VSGTIIF+VR A SWIIKN+P GEP
Sbjct: 121 VSTVWTFCFVMIGALVAPQNGAVAESVISLSTFIMVSGTIIFVVRQAMSWIIKNSPTGEP 180

Query: 247 LKEGFVVALLLGVLITAFSSKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVF 306
           LKEGFVVA+L+GVLITAFSSKAFGLHIYFGPLILG+SIPPGPPIGSALVERLDFITSWVF
Sbjct: 181 LKEGFVVAMLIGVLITAFSSKAFGLHIYFGPLILGISIPPGPPIGSALVERLDFITSWVF 240

Query: 307 MPVFFVKLGLVINVYSIKLKNFLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGL 366
           MPVFFVKLGLVINVYSIKLKNFLGVSFVIYVSALGKFLGAL+VAVV+CRMP+RDAVSLGL
Sbjct: 241 MPVFFVKLGLVINVYSIKLKNFLGVSFVIYVSALGKFLGALVVAVVHCRMPVRDAVSLGL 300

Query: 367 VMASQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKR 426
           VMASQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVL+AVITPIIRYLFDP+RRYTVYKR
Sbjct: 301 VMASQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVLMAVITPIIRYLFDPSRRYTVYKR 360

Query: 427 RTVMDLKPGSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQL 486
           RTVMDLK GSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQL
Sbjct: 361 RTVMDLKSGSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQL 420

Query: 487 IPHNLKKDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKT 546
           IPHNLKKDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVY LALNK+
Sbjct: 421 IPHNLKKDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYLLALNKS 480

Query: 547 VSLILIPFHKKFYSNGVISLSNYDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLH 606
           VSLIL+PFHKKFYSNGVISLSNY+MKMVNNEILDKAPCSVGLVVDRGVLNLSRS ATNLH
Sbjct: 481 VSLILVPFHKKFYSNGVISLSNYNMKMVNNEILDKAPCSVGLVVDRGVLNLSRSAATNLH 540

Query: 607 SFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQ 666
           SFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQ
Sbjct: 541 SFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQ 600

Query: 667 FRKATTNNRRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQ 726
           FRKATTNNRRVM IEEVVMDGTGTVSLLRSMGNSF LVIVGRRHSCC  LV+GLVLWNEQ
Sbjct: 601 FRKATTNNRRVMYIEEVVMDGTGTVSLLRSMGNSFSLVIVGRRHSCCSTLVQGLVLWNEQ 660

Query: 727 TELGAIGEVLASSDFMGNAIILVVQQHTKLANEEQNKNNEDS 768
           TELGAIGEVLASSDFMGNAIILVVQQHTKLANEEQNKNNEDS
Sbjct: 661 TELGAIGEVLASSDFMGNAIILVVQQHTKLANEEQNKNNEDS 702

BLAST of Cp4.1LG04g06100 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 463.8 bits (1192), Expect = 5.6e-130
Identity = 279/754 (37.00%), Postives = 449/754 (59.55%), Query Frame = 0

Query: 40  LLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTSFGTIFYFFLVG 99
           +LKP  QP ++S+ILGG+VLG S L     F  TIFP R  + L+ + + G +++ FLVG
Sbjct: 57  ILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVG 116

Query: 100 VQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNT---VGTRTAKSLLIVSAAES 159
           V++DI +++   K++L I    ++LP ++ + +S ++  +   +G  T   +L +  A S
Sbjct: 117 VEMDIMVVRKTGKRALTIAIGGMVLPFLIGAAFSFSMHRSEDHLGQGT--YILFLGVALS 176

Query: 160 FINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGAVADSVISL 219
              FP++A +L+EL L+N+E GRI++++++V+ ++    L +   +A  +     S+  +
Sbjct: 177 VTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVM 236

Query: 220 STFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEGFVVALLLGVLITAFSSKAFGLHIYFG 279
            +  V     +F+VR   +WII+  P GE   E  +  +L GV+I+ F + A G H  FG
Sbjct: 237 ISSAVFIAVCVFVVRPGIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFG 296

Query: 280 PLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIK-LKNFLGVSFVI 339
             + G+ IP G P+G  L+E+L+   S + +P+FF   GL  N+ +I+    +L +  VI
Sbjct: 297 AFVFGLVIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVI 356

Query: 340 YVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGRIDRGTFAV 399
           +++  GK +G ++VA  +  MP+R+ ++LGL++ ++G  E+ +  + + +  +D  TFA 
Sbjct: 357 FLACAGKVIGTVIVAFFH-GMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFAT 416

Query: 400 MCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGSDLRVLICIHDQEDVPNAIN 459
           M +  +V+  VITPI+  L+ P ++   YKRRT+   KP S+LRVL+C+H   +VP  IN
Sbjct: 417 MVLVALVMTGVITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIIN 476

Query: 460 LLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKD----RISTSCPSEHIVNAFKY 519
           LLEA +PT+RS + +Y+LHLVEL GRA++ LI HN +K        T   S+HI+NAF+ 
Sbjct: 477 LLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFEN 536

Query: 520 FGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISLSNYDM 579
           +   +   VA+ P T+I+  +TMH+DV SLA +K VS I+IPFHK+   +G +  +N   
Sbjct: 537 Y-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAY 596

Query: 580 KMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFVGARMA 639
           ++VN  +L+ +PCSVG++VDRG LN +    +N  S QVAV+F GG DDREA+    RMA
Sbjct: 597 RLVNQNLLENSPCSVGILVDRG-LNGATRLNSNTVSLQVAVLFFGGPDDREALAYAWRMA 656

Query: 640 GHPDINLTLIRLLKKGN-----------------VERDDVKETRLDNEAVLQFRKATTNN 699
            HP I LT++R +   +                  + D  K+ +LD++ +  FR      
Sbjct: 657 QHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEY 716

Query: 700 RRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGE 759
             ++ IE++V +G  TV+ +RSM +S DL IVGR       L  GL  W+E  ELGAIG+
Sbjct: 717 ESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGD 776

Query: 760 VLASSDFMGNAIILVVQQHTKLANEEQNKNNEDS 769
           +LASSDF     +LVVQQ+     +E + +  +S
Sbjct: 777 LLASSDFAATVSVLVVQQYVGSWAQEDDMDFPES 804

BLAST of Cp4.1LG04g06100 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 384.4 bits (986), Expect = 4.3e-106
Identity = 249/754 (33.02%), Postives = 422/754 (55.97%), Query Frame = 0

Query: 38  HQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGF--VFLDVVTSFGTIFYF 97
           H  L+ LG     S +L G++L  S L      R   F    +  +   +  +   + ++
Sbjct: 73  HFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAAR-RFFSTEDYKEIVFSLTAACSYMMFW 132

Query: 98  FLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTR----TAKSL--L 157
           FL+GV++D  +++   +K++ IG  SV+L  ++ S+     +  VGT+    T  SL  +
Sbjct: 133 FLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYV 192

Query: 158 IVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACF-------------- 217
           ++ + +   +FP+V +LL EL L NSE GR+A++S+++S   T+                
Sbjct: 193 VIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQ 252

Query: 218 -----LMIGTLVAPQNGAVADSVISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEG 277
                + IG ++A     +   ++ L  F+ ++   I++ R    +IIK  P+G P+K  
Sbjct: 253 TRLGSVFIGDVIAGNRPLMRAGIVVL--FVCIA---IYVFRPLMFYIIKQTPSGRPVKAI 312

Query: 278 FVVALLLGVLITAFSSKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVF 337
           ++  +++ V  +A  +      I+ GP ILG+++P GPP+GSA++++ +      F+P F
Sbjct: 313 YLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFF 372

Query: 338 FVKLGLVINVYSI-KLKNFLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMA 397
                  I++ ++   +   G+  ++  S + KF+   + A+ Y  MP+ D  +L L+M+
Sbjct: 373 IASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFY-GMPMEDCFALSLIMS 432

Query: 398 SQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTV 457
            +G FELG + L  + G +   TF V C+++ +  A+I PI+RYL+DP+R Y  Y++R +
Sbjct: 433 FKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNM 492

Query: 458 MDLKPGSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPH 517
             LKP S+LR+L CI+  +D+   INLLEA+ P+R S +  Y+LHL+EL+G+AN   I H
Sbjct: 493 QHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISH 552

Query: 518 NLKKDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSL 577
            L+  R   +  S +++ +F+ F ++   +V +  +T+++   TMH D+  LALN T SL
Sbjct: 553 KLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSL 612

Query: 578 ILIPFHKKFYSNGVISLSNYDM-KMVNNEILDKAPCSVGLVVDR---GVLNLS------R 637
           IL+PFH+ + ++G   +SN +M + +N  +LD APCSVG+ V R   G  N+S       
Sbjct: 613 ILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTIN 672

Query: 638 STATNLHSFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLLKKGNVERDD-VKETR 697
            T  NL S+ + ++FLGG DDREA+ +  RMA  P IN+T++RL+      R++ V +  
Sbjct: 673 GTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKM 732

Query: 698 LDNEAVLQFRKATTNNRRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVE 753
           LD+E +   +  T  +  +   E+ + D   T SLLRSM + FD+ IVGR +    +  E
Sbjct: 733 LDDELLRDVKSNTLVD--IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTE 792

BLAST of Cp4.1LG04g06100 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 379.4 bits (973), Expect = 1.4e-104
Identity = 243/777 (31.27%), Postives = 430/777 (55.34%), Query Frame = 0

Query: 36  LFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTSFGTIFYF 95
           L ++LLKPL Q +I +Q+L G++LG S       +     P+ G + L  +++ G   + 
Sbjct: 66  LLYRLLKPLKQGMISAQVLAGIILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHL 125

Query: 96  FLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNT--VGTRTAKSLLIVSA 155
           FL+G+++D SI++    K++ IGT S  LP  L +L    L NT  +       +  V +
Sbjct: 126 FLLGLRIDASIIRKAGSKAILIGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVIS 185

Query: 156 AESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGAVAD-- 215
             +  +FP+  ++L+EL+++NS+ GR+A   S+V   ++    ++  +   ++G +A   
Sbjct: 186 LNAMTSFPVTTTVLAELNILNSDLGRLATNCSIVCEAFSWIVALVFRMFL-RDGTLASVW 245

Query: 216 SVISLSTFIVVSGTIIFIVRLATSWIIKNNPAG-EPLKEGFVVALLLGVLITAFSSKAFG 275
           S + ++  I+V   I F+ R A  W+ +      +   E     +++ +L  + +S+  G
Sbjct: 246 SFVWVTALILV---IFFVCRPAIIWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLG 305

Query: 276 LHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLKNFLG 335
           +H  FG   LGVS+P GPP+G+ L  +L+   + + +P F    GL  N + I   +   
Sbjct: 306 VHAAFGAFWLGVSLPDGPPLGTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKI 365

Query: 336 VSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGRIDR 395
           +  VI ++   KFLG    A  YC + + DA SL L+M  QG  E+    + + E  ++ 
Sbjct: 366 IEAVILITYGCKFLGT-AAASAYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNT 425

Query: 396 GTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGS-DLRVLICIHDQED 455
             F ++ I L+++  +   ++  L+DP++RY    +RT++D +  +   R+L+C+++ E+
Sbjct: 426 ECFNLLIITLLLVTGISRFLVVCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVEN 485

Query: 456 VPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIVNAF 515
           VP+ +NLLEA  P+R S + V+ LHLVEL GRA++ L+PH+        +  S HIVN F
Sbjct: 486 VPSMVNLLEASYPSRFSPISVFTLHLVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGF 545

Query: 516 KYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISLSNY 575
           + F + N+ T+    FT+    ++++DD+ +LAL+K  +LI+IPFHK++  +G +   N 
Sbjct: 546 QRFEQQNQGTLMAQHFTAAAPFSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNP 605

Query: 576 DMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFVGAR 635
            ++ +N  +L+KAPCSVG+ +DRG     RS   +     VAV+F+ G DD EA+    R
Sbjct: 606 SIRNINLNVLEKAPCSVGIFIDRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMR 665

Query: 636 MAGHPDINLTLIRLLKKGNVERD---DVKETRLDNEAVLQFRKATTNNRRVMCIEEVVMD 695
           +A HP++++T+I    K +++++   DV+    ++  +  F+    +  ++   EE+V D
Sbjct: 666 IAEHPEVSVTMIHFRHKSSLQQNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRD 725

Query: 696 GTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNAI 755
           G  T  ++ S+G+SFDLV+VGR H     ++ GL  W+E  ELG IG++ ASSDF  + +
Sbjct: 726 GVETTQVISSLGDSFDLVVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVL 785

Query: 756 ILVVQQHTKLANEEQNKNNEDSSQVRIPRSKMGSIVMEPGDNSIFNNFSSICVHVDR 804
           ++  Q+   LA +   K      +V  PR       + P   S+   F+S+ +H +R
Sbjct: 786 VIHQQEGDSLAMDNSYKLPASPHRVGDPR-------VHP-RFSVEEGFTSVDLHSNR 829

BLAST of Cp4.1LG04g06100 vs. TAIR 10
Match: AT3G44900.1 (cation/H+ exchanger 4 )

HSP 1 Score: 360.5 bits (924), Expect = 6.7e-99
Identity = 238/757 (31.44%), Postives = 421/757 (55.61%), Query Frame = 0

Query: 37  FHQLLKPLGQPLIVSQILGGLVLGSSGLSH---------MKTFRDTIFPLRGFVFLDVVT 96
           FH  L+ LG     S +L G++L  S L            + +++T+F L G        
Sbjct: 71  FHFFLRRLGMIRFTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLFGLVG-------- 130

Query: 97  SFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAK- 156
           +   + ++FL+GV++D+S++++  +K++ IG  SV+L + + +L    ++  VGT+  + 
Sbjct: 131 ACSYMMFWFLMGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEP 190

Query: 157 -----SLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVS----TIWTACFLM 216
                 ++ +   +   +FP++ +LL EL L NSE GR+A++S+++S    +I +A  + 
Sbjct: 191 VMSFFEIIFIYLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVF 250

Query: 217 IGTLVAPQNGA----VADSVIS------LSTFIVVSGTIIFIVRLATSWIIKNNPAGEPL 276
           +  L   ++      + D ++         T ++     I+I R    +IIK  P+G P+
Sbjct: 251 LKELKDDKSRLGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPV 310

Query: 277 KEGFVVALLLGVLITAFSSKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFM 336
           K+ ++ A+++ V  +A  +      I+ GP ILG+++P GPP+GSA++++ + +    F+
Sbjct: 311 KKFYIYAIIILVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFL 370

Query: 337 PVFFVKLGLVINVYSIK-LKNFLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGL 396
           P F       I+   ++   +   +  ++ VS + KF    L A +Y  MP +D ++L L
Sbjct: 371 PFFVATSAEEIDTSILQSWIDLKSIVILVSVSFIVKFALTTLPAFLY-GMPAKDCIALSL 430

Query: 397 VMASQGAFELGMFKLMRKEGRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKR 456
           +M+ +G FE G +    + G I   TF V+ +++++  AVI P+++ ++DP+R Y  Y++
Sbjct: 431 IMSFKGIFEFGAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEK 490

Query: 457 RTVMDLKPGSDLRVLICIHDQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQL 516
           R ++ +KP S+LR+L CI+  +D+   INLLEA  P+R + +  Y+LHL+EL+G+AN  L
Sbjct: 491 RNMLHMKPNSELRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVL 550

Query: 517 IPHNLK-KDRISTSCPSEHIVNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNK 576
           I H L+ +   + S  SE++V +F+ F  +   +V +  +T+++    MH D+  LALN 
Sbjct: 551 ISHRLQTRKSENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMMHGDICMLALNN 610

Query: 577 TVSLILIPFHKKFYSNGVISLSNYDM-KMVNNEILDKAPCSVGLVVDRGV--LNLSRSTA 636
           T SLI++PFH+ + ++G   +S+  M + +N  +LD +PCSVG+ V R        + TA
Sbjct: 611 TTSLIILPFHQTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGRRTIKETA 670

Query: 637 TNLHSFQVAVVFLGGADDREAMFVGARMAGHPDINLTLIRLL---KKGNVERDDVKETRL 696
            N  S+QV ++FLGG DDREA+ +  RMA    I +T++ L+   ++ N   D   +  L
Sbjct: 671 ANFSSYQVCMLFLGGKDDREALSLAKRMARDSRITITVVSLISSEQRANQATD--WDRML 730

Query: 697 DNEAVLQFRKATTNNRRVMCIEEVVMDGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEG 756
           D E +   +        ++  EEVV D   T  LL+S+ N +DL IVGR      +  EG
Sbjct: 731 DLELLRDVKSNVLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVFTEG 790

BLAST of Cp4.1LG04g06100 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 357.1 bits (915), Expect = 7.4e-98
Identity = 237/755 (31.39%), Postives = 404/755 (53.51%), Query Frame = 0

Query: 36  LFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDVVTSFGTIFYF 95
           L  ++L+PL Q +I +Q+L G+VLG S L H   + +   P  G + +  +++ G + + 
Sbjct: 68  LIFRVLQPLKQGMISAQVLTGVVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHL 127

Query: 96  FLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTAKSLLIVSA-- 155
           FL+G+++D SI++    K++ IGT S   P  L +L    +  T+G  +       SA  
Sbjct: 128 FLLGLKIDGSIIRKAGSKAILIGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAIS 187

Query: 156 AESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQNGAVADSV 215
             S  +FP+  ++L+EL+++NSE GR+A   SMV  + +    +   L          S+
Sbjct: 188 LSSMTSFPVTTTVLAELNILNSELGRLATHCSMVCEVCSWFVALAFNLYT--RDRTMTSL 247

Query: 216 ISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKE---GFVVALLLGVLITAFSSKAFG 275
            +LS  I +   I F+ R    W+ +        K+    F V LLL   I + S +A G
Sbjct: 248 YALSMIIGLLLVIYFVFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLS--IASLSGEAMG 307

Query: 276 LHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKLK---N 335
           +H  FG   LGVS+P GPP+G+ L  +L+   S +F+P F    GL  N + I      +
Sbjct: 308 VHAAFGAFWLGVSLPDGPPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHH 367

Query: 336 FLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKEGR 395
            + +  ++ ++   KFLG    A  YC+  + DA+ L  +M  QG  E+    + +    
Sbjct: 368 VVMIEIILLITYGCKFLGT-AAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQV 427

Query: 396 IDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGS-DLRVLICIHD 455
           +D   F ++ I ++ +  +   ++ YL+DP++RY    +RT+++ +  +  LR+L+ +++
Sbjct: 428 VDTECFNLVIITILFVTGISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYN 487

Query: 456 QEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHIV 515
            E+VP+ +NLLEA  PTR + +  + LHLVEL GRA++ L PH+        +  S HIV
Sbjct: 488 VENVPSMVNLLEATYPTRFNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIV 547

Query: 516 NAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVISL 575
           NAF+ F +  +  +    FT+    +++++D+ +LAL+K  +LI+IPFHK++  +G +  
Sbjct: 548 NAFQRFEQKYQGALMAQHFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQ 607

Query: 576 SNYDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMFV 635
            N  ++ +N  +LD APCSV + +DRG     RS         VA++F+GG DD EA+ +
Sbjct: 608 VNGPIRTINLNVLDAAPCSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALAL 667

Query: 636 GARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMCIEEVVMD 695
             RMA  PD+N+T+I    K  ++ +D  +   +   +  F+    N  ++  +EE+V D
Sbjct: 668 CMRMAEKPDLNVTMIHFRHKSALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRD 727

Query: 696 GTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNAI 755
           G  T  ++ S+G+++D+V+VGR H     ++ GL  W+E  ELG IG++L S DF  + +
Sbjct: 728 GVETTQVISSLGDAYDMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVL 787

Query: 756 ILVVQQ---------HTKLANEEQNKNNEDSSQVR 773
           ++  QQ           KL N E  K  +   Q R
Sbjct: 788 VVHQQQGDDLLAMDDSYKLPNVEHQKIGDTGIQQR 816