Cp4.1LG04g05030 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g05030
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMajor facilitator superfamily protein
LocationCp4.1LG04: 6224377 .. 6225568 (+)
RNA-Seq ExpressionCp4.1LG04g05030
SyntenyCp4.1LG04g05030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCATCTTTCACCCCCCTCCTCCGCCGTCCTCCTCCTCCGCCGTGAAGTGGCTCAGCCTAGTCTCCGCCGTCTGGGTCCAATCCATCGCAGGCAACAACTACACCTTCTCCAACTACTCCGCCGCCCTCAAATCCCTCATGAACCTCTCCCAACTCCAACTCAACAATCTCTCCGTCGCCAAAGACTTAGGCAAAGCCTTTGGCCTCCTTTCCGGCCTCGCCTCCGACCGCCTCCCCCCCTCCCTCCTCCTCCTCATCGGCTCCCTCGAAGGCCTTATCGGCTACGGCGGACAATGGCTCGTCGTCAGCAAAACAATCAACCCCCTCCCTTACTGGCAGGTCAATCTCTGTTTCAATTTCTGTAATGAAATGAAAATTTTCGGCTATTTCTTGATTTGGGTTGGTTTTAATTGTATAGATAAGTATTTTTATGTGCATGGGAGGGAATAGTAGCACTTGGATGAACACCGCCGTGCTCGTTACTTGCCTCAGAAACTTCCGGCGAAACATAGGCTCTGTTTCCGGCGTTCTCAAAGGCTACGTCGCTCTCAGCACCGCCATTTTCACCGATCTCTGTTTCGCCCTTTTCTCCAATGATCCCTCTTCCTTTCTCGCCCTTCTCGCCGTCGTCCCCTTCGCCGTTTGCCTCATTGCCATTCTCTTCCTCCGTGAAACCCCCTTCGCCGTCGATAATGAAACAGAGGATTCCCAATACTTCTGGGTCCTCAACGCTATCGCCGTCGCCGTCGCCGTCAGTCTCTTGGCCTTTGATTCGATCCCCAATCCCACCTCATCCCTATCGATAATTTTCTCCATTGTGTTATTGATTCTACTCGTTTCCCCTCTGGTAATCCCAATTCACTCATTCCTCAAGAACAGGGGCCGGTCCGGTGCGGCTGTAGAGGCGTTGTTGGCGGCTGAGGAAGTGGTGGAGGTGGAGGGAAATGGGAATCCGGTGATAGGAGAAGATCACACGATTGTGGAGGCGATGAAGACGGTTGAATTTTGGCTTCTGTTTGTGTCGTTTTTGTTTGGAGTGGGTACCGGATTGGCAGTGATGAACAACATGGGTCAAATCGGATTGGCTCTTGGATACGACGACGTTTCGAGCTTCATCTCTTTGATGAGCATTTGGGGTTTTTTTGGTCGGATTTTATCGGGATCGGCGTCGGAGTATTTCATCAAGTAA

mRNA sequence

ATGAGCATCTTTCACCCCCCTCCTCCGCCGTCCTCCTCCTCCGCCGTGAAGTGGCTCAGCCTAGTCTCCGCCGTCTGGGTCCAATCCATCGCAGGCAACAACTACACCTTCTCCAACTACTCCGCCGCCCTCAAATCCCTCATGAACCTCTCCCAACTCCAACTCAACAATCTCTCCGTCGCCAAAGACTTAGGCAAAGCCTTTGGCCTCCTTTCCGGCCTCGCCTCCGACCGCCTCCCCCCCTCCCTCCTCCTCCTCATCGGCTCCCTCGAAGGCCTTATCGGCTACGGCGGACAATGGCTCGTCGTCAGCAAAACAATCAACCCCCTCCCTTACTGGCAGATAAGTATTTTTATGTGCATGGGAGGGAATAGTAGCACTTGGATGAACACCGCCGTGCTCGTTACTTGCCTCAGAAACTTCCGGCGAAACATAGGCTCTGTTTCCGGCGTTCTCAAAGGCTACGTCGCTCTCAGCACCGCCATTTTCACCGATCTCTGTTTCGCCCTTTTCTCCAATGATCCCTCTTCCTTTCTCGCCCTTCTCGCCGTCGTCCCCTTCGCCGTTTGCCTCATTGCCATTCTCTTCCTCCGTGAAACCCCCTTCGCCGTCGATAATGAAACAGAGGATTCCCAATACTTCTGGGTCCTCAACGCTATCGCCGTCGCCGTCGCCGTCAGTCTCTTGGCCTTTGATTCGATCCCCAATCCCACCTCATCCCTATCGATAATTTTCTCCATTGTGTTATTGATTCTACTCGTTTCCCCTCTGGTAATCCCAATTCACTCATTCCTCAAGAACAGGGGCCGGTCCGGTGCGGCTGTAGAGGCGTTGTTGGCGGCTGAGGAAGTGGTGGAGGTGGAGGGAAATGGGAATCCGGTGATAGGAGAAGATCACACGATTGTGGAGGCGATGAAGACGGTTGAATTTTGGCTTCTGTTTGTGTCGTTTTTGTTTGGAGTGGGTACCGGATTGGCAGTGATGAACAACATGGGTCAAATCGGATTGGCTCTTGGATACGACGACGTTTCGAGCTTCATCTCTTTGATGAGCATTTGGGGTTTTTTTGGTCGGATTTTATCGGGATCGGCGTCGGAGTATTTCATCAAGTAA

Coding sequence (CDS)

ATGAGCATCTTTCACCCCCCTCCTCCGCCGTCCTCCTCCTCCGCCGTGAAGTGGCTCAGCCTAGTCTCCGCCGTCTGGGTCCAATCCATCGCAGGCAACAACTACACCTTCTCCAACTACTCCGCCGCCCTCAAATCCCTCATGAACCTCTCCCAACTCCAACTCAACAATCTCTCCGTCGCCAAAGACTTAGGCAAAGCCTTTGGCCTCCTTTCCGGCCTCGCCTCCGACCGCCTCCCCCCCTCCCTCCTCCTCCTCATCGGCTCCCTCGAAGGCCTTATCGGCTACGGCGGACAATGGCTCGTCGTCAGCAAAACAATCAACCCCCTCCCTTACTGGCAGATAAGTATTTTTATGTGCATGGGAGGGAATAGTAGCACTTGGATGAACACCGCCGTGCTCGTTACTTGCCTCAGAAACTTCCGGCGAAACATAGGCTCTGTTTCCGGCGTTCTCAAAGGCTACGTCGCTCTCAGCACCGCCATTTTCACCGATCTCTGTTTCGCCCTTTTCTCCAATGATCCCTCTTCCTTTCTCGCCCTTCTCGCCGTCGTCCCCTTCGCCGTTTGCCTCATTGCCATTCTCTTCCTCCGTGAAACCCCCTTCGCCGTCGATAATGAAACAGAGGATTCCCAATACTTCTGGGTCCTCAACGCTATCGCCGTCGCCGTCGCCGTCAGTCTCTTGGCCTTTGATTCGATCCCCAATCCCACCTCATCCCTATCGATAATTTTCTCCATTGTGTTATTGATTCTACTCGTTTCCCCTCTGGTAATCCCAATTCACTCATTCCTCAAGAACAGGGGCCGGTCCGGTGCGGCTGTAGAGGCGTTGTTGGCGGCTGAGGAAGTGGTGGAGGTGGAGGGAAATGGGAATCCGGTGATAGGAGAAGATCACACGATTGTGGAGGCGATGAAGACGGTTGAATTTTGGCTTCTGTTTGTGTCGTTTTTGTTTGGAGTGGGTACCGGATTGGCAGTGATGAACAACATGGGTCAAATCGGATTGGCTCTTGGATACGACGACGTTTCGAGCTTCATCTCTTTGATGAGCATTTGGGGTTTTTTTGGTCGGATTTTATCGGGATCGGCGTCGGAGTATTTCATCAAGTAA

Protein sequence

MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSLSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILSGSASEYFIK
Homology
BLAST of Cp4.1LG04g05030 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.5e-41
Identity = 116/373 (31.10%), Postives = 195/373 (52.28%), Query Frame = 0

Query: 17  KWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLAS 76
           KW  LV+A+W+Q+  G N+ FS YS+ LKS++ +SQ++LN L+VA DLGKAFG  SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 77  DRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVT 136
              P S++L   +  G +GYG QWLV++  I  LPY  + +   + G S  W NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIIT-LPYSLVFLCCLLAGLSICWFNTACFIL 162

Query: 137 CLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILF 196
           C+R+F  N      +   +  +S A+++    A+  +  + +L L ++VP  V   A+  
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYP 222

Query: 197 LRETPFA-----VDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSLSIIFSIVLLI 256
           +   P        D+   DS  F +LN +AV  +  LL   S    ++ L+ I ++VLL+
Sbjct: 223 VLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL-LSSSSTSSARLNFIGAVVLLV 282

Query: 257 L-LVSPLVI-------PIHSFLKNRGRSG-------------AAVEALLAAEEVVEVEGN 316
             L +PL++       P+ +   N   SG              +V +    E +   +  
Sbjct: 283 FPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEG 342

Query: 317 GNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLM 364
               +G++H+    +  +EFWL ++++  G   GL   NN+GQI  +LG +  ++ +++ 
Sbjct: 343 NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS-TTLVTIY 402

BLAST of Cp4.1LG04g05030 vs. NCBI nr
Match: XP_023529669.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 710 bits (1833), Expect = 2.87e-255
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT
Sbjct: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASEYFIK
Sbjct: 361 SGSASEYFIK 370

BLAST of Cp4.1LG04g05030 vs. NCBI nr
Match: KAG6589004.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 702 bits (1811), Expect = 2.88e-254
Identity = 365/370 (98.65%), Postives = 367/370 (99.19%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRL PSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLLPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASEYF+K
Sbjct: 361 SGSASEYFVK 370

BLAST of Cp4.1LG04g05030 vs. NCBI nr
Match: XP_022928343.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 706 bits (1822), Expect = 1.36e-253
Identity = 367/370 (99.19%), Postives = 368/370 (99.46%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASEYFIK
Sbjct: 361 SGSASEYFIK 370

BLAST of Cp4.1LG04g05030 vs. NCBI nr
Match: XP_022989043.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 694 bits (1792), Expect = 4.82e-249
Identity = 364/370 (98.38%), Postives = 365/370 (98.65%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSS AVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSS-AVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLP SLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFD IPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASEYFIK
Sbjct: 361 SGSASEYFIK 369

BLAST of Cp4.1LG04g05030 vs. NCBI nr
Match: KAG7015269.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675 bits (1741), Expect = 5.42e-242
Identity = 353/364 (96.98%), Postives = 357/364 (98.08%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRR+IGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRSIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWG--FFGR 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWG  + G 
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGSLYIGS 360

Query: 361 ILSG 362
           I+ G
Sbjct: 361 IIVG 364

BLAST of Cp4.1LG04g05030 vs. ExPASy TrEMBL
Match: A0A6J1ENR3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435199 PE=4 SV=1)

HSP 1 Score: 706 bits (1822), Expect = 6.56e-254
Identity = 367/370 (99.19%), Postives = 368/370 (99.46%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASEYFIK
Sbjct: 361 SGSASEYFIK 370

BLAST of Cp4.1LG04g05030 vs. ExPASy TrEMBL
Match: A0A6J1JP36 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486233 PE=4 SV=1)

HSP 1 Score: 694 bits (1792), Expect = 2.33e-249
Identity = 364/370 (98.38%), Postives = 365/370 (98.65%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           MSIFHPPPPPSSS AVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSS-AVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLLSGLASDRLP SLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC
Sbjct: 61  AKDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFD IPNPTSS
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSS 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           +SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAA EALLAAEEV EVEGNGNPVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASEYFIK
Sbjct: 361 SGSASEYFIK 369

BLAST of Cp4.1LG04g05030 vs. ExPASy TrEMBL
Match: A0A5A7U1S8 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00720 PE=4 SV=1)

HSP 1 Score: 567 bits (1461), Expect = 2.66e-201
Identity = 293/370 (79.19%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           M+IF   PP  SSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSL+NLSQLQLNNLSV
Sbjct: 1   MNIFRRSPP--SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLL+GL+SDRL  SLLL IGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C
Sbjct: 61  AKDLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFRR+ G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLA
Sbjct: 121 MGGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LL++VPF VCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FDSIPNP S 
Sbjct: 181 LLSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           LS IFSI+LL LL SPLVIP+HSFLKNRG SG   EALLA E   +    G P IGEDHT
Sbjct: 241 LSRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGY+DVS FISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASE+FIK
Sbjct: 361 SGSASEHFIK 368

BLAST of Cp4.1LG04g05030 vs. ExPASy TrEMBL
Match: A0A0A0K0K5 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G011810 PE=4 SV=1)

HSP 1 Score: 566 bits (1458), Expect = 7.62e-201
Identity = 293/370 (79.19%), Postives = 325/370 (87.84%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           M+ F   PP  S SA+KWL LV+A+WVQSI+GNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MNSFRRSPP--SPSALKWLGLVTAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLL+GLASDRL  SLLLLIGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C
Sbjct: 61  AKDLGKAFGLLAGLASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLC 120

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFR+N G+VSG+LKGY+ALSTAIFTD C ALFS++PSSFLA
Sbjct: 121 MGGNSTTWMNTGVLVTCLRNFRKNRGTVSGILKGYIALSTAIFTDFCSALFSDNPSSFLA 180

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LL++VPFAVCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FDSIPNP S 
Sbjct: 181 LLSLVPFAVCLAAILFLREVPRSADDETEEFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 240

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           LS IF IVLL LLVSPLVIP+HSFLKN+G SG   EALLA E   +    G PVIGEDHT
Sbjct: 241 LSRIFCIVLLSLLVSPLVIPLHSFLKNKGGSGEVAEALLAGESGADNAVEGKPVIGEDHT 300

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKT EFW++FVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISLMSIWGFFGRIL
Sbjct: 301 IVEAMKTFEFWIMFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRIL 360

Query: 361 SGSASEYFIK 370
           SGSASE+FIK
Sbjct: 361 SGSASEHFIK 368

BLAST of Cp4.1LG04g05030 vs. ExPASy TrEMBL
Match: A0A1S3BI29 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103490296 PE=4 SV=1)

HSP 1 Score: 567 bits (1461), Expect = 1.18e-198
Identity = 293/370 (79.19%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           M+IF   PP  SSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSL+NLSQLQLNNLSV
Sbjct: 25  MNIFRRSPP--SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSV 84

Query: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120
           AKDLGKAFGLL+GL+SDRL  SLLL IGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C
Sbjct: 85  AKDLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLC 144

Query: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFRR+ G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLA
Sbjct: 145 MGGNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLA 204

Query: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240
           LL++VPF VCL AILFLRE P + D+ETE+ +YFWVLNA++VAVAV+LL FDSIPNP S 
Sbjct: 205 LLSLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSF 264

Query: 241 LSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAVEALLAAEEVVEVEGNGNPVIGEDHT 300
           LS IFSI+LL LL SPLVIP+HSFLKNRG SG   EALLA E   +    G P IGEDHT
Sbjct: 265 LSRIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHT 324

Query: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360
           IVEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGY+DVS FISLMSIWGFFGRIL
Sbjct: 325 IVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRIL 384

Query: 361 SGSASEYFIK 370
           SGSASE+FIK
Sbjct: 385 SGSASEHFIK 392

BLAST of Cp4.1LG04g05030 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 474.9 bits (1221), Expect = 5.7e-134
Identity = 254/385 (65.97%), Postives = 299/385 (77.66%), Query Frame = 0

Query: 11  SSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL 70
           SSSSA+KWL  V+AVWVQSI+GNNYTFSNYS ALKSLMNL+QL+LNNLSVAKD+GKAFG+
Sbjct: 8   SSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVGKAFGI 67

Query: 71  LSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMN 130
           L+GLASDRLP  ++LLIG  EGL+GYG QWLVVS+TI P+PYWQ+ IF+CMGGNS+TWMN
Sbjct: 68  LAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNSTTWMN 127

Query: 131 TAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVC 190
           TAVLVTC+RNFRRN G VSG+LKGYV LSTAIFTDLC ALFSNDP+SFL LLAVVPFAVC
Sbjct: 128 TAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLAVVPFAVC 187

Query: 191 LIAILFLRETPFA--VDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSLSIIFSIV 250
           L A+ FLRE P A     E E+++YF + N +AV VAV L ++D I   T   S+ F+ +
Sbjct: 188 LTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVFSVAFASI 247

Query: 251 LLILLVSPLVIPIHSFLKN------------------RGRSGAA-----VEALLAAEEVV 310
           LL LL SP+ IP HSF+K+                  R    AA     V A +AAEE  
Sbjct: 248 LLFLLASPIAIPFHSFIKSLNYGEQDDLEGRIQEPLLRSEIAAAEKEVIVVAAVAAEE-- 307

Query: 311 EVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSS 370
           E +    PV+GEDHTI+EA+ TV+FW+LFVSFL GVGTGLAVMNNMGQIGLALGY +VS 
Sbjct: 308 EEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYTNVSI 367

BLAST of Cp4.1LG04g05030 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.5e-131
Identity = 254/404 (62.87%), Postives = 307/404 (75.99%), Query Frame = 0

Query: 11  SSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL 70
           SSSSA+KWL  V+AVWVQSI+GNNYTFSNYS ALKSLMNL+QL+LN+LSVAKD+GKAFG+
Sbjct: 10  SSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAFGI 69

Query: 71  LSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMN 130
           L+GLASDRL   ++LLIGS EGL+GYG QWLVVS+TI P+PYWQ+ +F+CMGGNS+TWMN
Sbjct: 70  LAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGNSTTWMN 129

Query: 131 TAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVC 190
           TAVLVTC+RNFRRN G VSG+LKGYV LSTAIFTDLC ALFS+DP+SFL LL+VVPFAVC
Sbjct: 130 TAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSVVPFAVC 189

Query: 191 LIAILFLRETP----FAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSSLSIIFS 250
           L A+ FLRE P    FA DN  E+S+YF V N +AV VAV L ++D I   T + SI F+
Sbjct: 190 LTAVFFLREIPPSTTFAEDN--EESKYFAVFNIVAVVVAVYLQSYDIIGIKTGAFSIAFA 249

Query: 251 IVLLILLVSPLVIPIHSFLKNRG---------------RSGAAVE-------ALLAAEEV 310
            +LLILL SP+ +P H+F++++                RSG+ +E       A  AA+  
Sbjct: 250 SILLILLASPVAVPFHAFIRSKVHDEQDVEGRIDEPLLRSGSEIEVEETIVGAAAAADNE 309

Query: 311 V----------EVEGNG--------NPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLA 370
           +          E E +G         PV+GE+HTI+EAM TV+FW+LFVSFL GVGTGLA
Sbjct: 310 LPPSLKPLSNEEEENHGTIVTTEKKRPVLGEEHTIMEAMLTVDFWVLFVSFLCGVGTGLA 369

BLAST of Cp4.1LG04g05030 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 212.2 bits (539), Expect = 6.9e-55
Identity = 136/414 (32.85%), Postives = 212/414 (51.21%), Query Frame = 0

Query: 17  KWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLAS 76
           +WL  V+A+W+QS AG  Y F + S  +KS +N +Q QL+ L VAKDLG + G L+G  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 77  DRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVT 136
           + LP    LL+GS++ L+GYG  WL+V+     LP W + I + +G N  T+ NTA LV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 137 CLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILF 196
            ++NF ++ G V G+LKG+  L  AI + +   + S+D +S + ++AV P  V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 197 LRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLA---FDSIPNPTSSLSIIFSIVLLILL 256
           +R         + D+  F V+ A+ + +A  L+A    +   + + S+ I F++VL  +L
Sbjct: 196 IRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLFAIL 255

Query: 257 VSPLVIPIHS--FLKNRGRSGAAVEALL-------------------------------- 316
           + P+ IPI +  F  +        E LL                                
Sbjct: 256 LVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEKPKE 315

Query: 317 -----------------------AAEEVVEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVS 371
                                  AAE  V V+    P  GED T+ +A+   +FWL+F S
Sbjct: 316 VDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLIFFS 375

BLAST of Cp4.1LG04g05030 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 206.1 bits (523), Expect = 5.0e-53
Identity = 127/409 (31.05%), Postives = 207/409 (50.61%), Query Frame = 0

Query: 17  KWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLSGLAS 76
           +WL  V+A+W+QS AG  Y F + S  +KS +N +Q +L+ L VAKDLG + G ++G  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 77  DRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNTAVLVT 136
           + LP    LL+G+++ LIGYG  WL+V+     LP W + + + +G N  T+ NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 137 CLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCLIAILF 196
            ++NF ++ G V G+LKG+  L  AI + +   + S++P+S + ++AV P  V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 197 LRETPFAVDNETEDSQYFWVLNAIAVAVA---VSLLAFDSIPNPTSSLSIIFSIVLLILL 256
           +R           D   F  +  + + +A   +S++    +   + ++  +F+IVL ++L
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVIL 255

Query: 257 VSPLVIPI-HSFLKNRGRSGAAVEALL--------------------------------- 316
           V P+++PI  SF          +E  L                                 
Sbjct: 256 VVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLLP 315

Query: 317 ------------------AAEEVVEVEGNGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGV 371
                             AAE  V V     P  GED T+ +A+   +FWL+F S L G 
Sbjct: 316 ASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLGS 375

BLAST of Cp4.1LG04g05030 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 2.1e-51
Identity = 134/400 (33.50%), Postives = 198/400 (49.50%), Query Frame = 0

Query: 12  SSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLL 71
           S S   W+ L +A WVQ  AG+  TF  YS+ALKS++  SQ Q+  L VA DLG+  GLL
Sbjct: 8   SGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDLGENMGLL 67

Query: 72  SGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCMGGNSSTWMNT 131
            G AS++LPP  +LLIG+    +G+G  WL VS+ +  LP+W + + + +  NS++W  T
Sbjct: 68  PGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATNSNSWFGT 127

Query: 132 AVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLALLAVVPFAVCL 191
           A LVT +RNF  + G V+G+LKGY+ +S A FT L   +  +     L  L V    +CL
Sbjct: 128 ASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTVGIPVICL 187

Query: 192 IAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNP---TSSLSIIFSIV 251
             + F+R    A   +  +  YF  L   ++  A  L+    +       S L  +   +
Sbjct: 188 TVMYFIRPCIPATGEDPSEPMYFAFLLVTSILFAAYLVVTTVLSEVFILPSILKYVLVAI 247

Query: 252 LLILLVSPLVIPIHS--FLKNRGRSGAAVEALLAAEE----------------------- 311
           +++LL+SPL +PI    F  N   S       LA EE                       
Sbjct: 248 MVLLLLSPLAVPIKMTLFRSNAKSSPLGSSDNLAKEEGTHEEPLLTPSTSASNLGPIFEG 307

Query: 312 ---------VVEVEG----NGNPVIGEDHTIVEAMKTVEFWLLFVSFLFGVGTGLAVMNN 371
                    + E EG       P  GED    +     +FWLL+  +  G+G+G+ V NN
Sbjct: 308 DDESDMEILLAEAEGAVKKKRKPRRGEDFKFGQVFVKADFWLLWFVYFLGMGSGVTVSNN 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E11.5e-4131.10Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_023529669.12.87e-255100.00protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
KAG6589004.12.88e-25498.65Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022928343.11.36e-25399.19protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_022989043.14.82e-24998.38protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
KAG7015269.15.42e-24296.98Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
A0A6J1ENR36.56e-25499.19protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JP362.33e-24998.38protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486... [more]
A0A5A7U1S82.66e-20179.19Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0K0K57.62e-20179.19Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G01181... [more]
A0A1S3BI291.18e-19879.19protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103490296 ... [more]
Match NameE-valueIdentityDescription
AT2G16660.15.7e-13465.97Major facilitator superfamily protein [more]
AT4G34950.14.5e-13162.87Major facilitator superfamily protein [more]
AT3G01930.26.9e-5532.85Major facilitator superfamily protein [more]
AT5G14120.15.0e-5331.05Major facilitator superfamily protein [more]
AT1G80530.12.1e-5133.50Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 17..261
e-value: 8.4E-86
score: 287.5
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 15..211
e-value: 3.8E-7
score: 31.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 8..368
NoneNo IPR availablePANTHERPTHR21576:SF106MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 10..277
NoneNo IPR availablePANTHERPTHR21576:SF106MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 289..370
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 289..370
coord: 10..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g05030.1Cp4.1LG04g05030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane