Cp4.1LG04g01900 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g01900
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmitochondrial import inner membrane translocase subunit TIM23-2-like
LocationCp4.1LG04: 212033 .. 212584 (-)
RNA-Seq ExpressionCp4.1LG04g01900
SyntenyCp4.1LG04g01900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATTCCCCGCAATCAGCCGGTGATCGGAAGACCCGTTTCTACCACCCCTACCAAGACCTTCACGTCCCGATCACCAAGCTTTACGAGCTTCCCACCGCCCCAGAACATCTTTTCTTCGAAGAGGCGGCTAGGCCCCACCGTTCTTGGGGCGAAAATCTCCAGTACTACACCGGAATCGGCTACTTGTCCGGTGCCCTTTTTGGCGGCGCCAGGGGATCTCTCCAGGGCATTAGGGCGGCGGAGCCTGGCGACACCATGAAGCTTCGTCTTAATCGAGTTTTGAACTCTGGGGGTCAGGTCGGCCGCAGGGCTGGGAACTCTCTTGGGATTCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTACTCATTTGAGAGGCTCTGATGATGTTTTGAATAGTATTGTTGCTGGATTGGGGACTGGTGCAATTTATAAGGCGGCTTCAGGGCCGAGGTCGGCCGCCATCGCTGGTGCTATTGGAGGGATTGCTGCTGCAGCTGCGGTGGCGGGCAAGCAGGCAGCGAAGAGATATGTGCCAATATAG

mRNA sequence

ATGGAAGATTCCCCGCAATCAGCCGGTGATCGGAAGACCCGTTTCTACCACCCCTACCAAGACCTTCACGTCCCGATCACCAAGCTTTACGAGCTTCCCACCGCCCCAGAACATCTTTTCTTCGAAGAGGCGGCTAGGCCCCACCGTTCTTGGGGCGAAAATCTCCAGTACTACACCGGAATCGGCTACTTGTCCGGTGCCCTTTTTGGCGGCGCCAGGGGATCTCTCCAGGGCATTAGGGCGGCGGAGCCTGGCGACACCATGAAGCTTCGTCTTAATCGAGTTTTGAACTCTGGGGGTCAGGTCGGCCGCAGGGCTGGGAACTCTCTTGGGATTCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTACTCATTTGAGAGGCTCTGATGATGTTTTGAATAGTATTGTTGCTGGATTGGGGACTGGTGCAATTTATAAGGCGGCTTCAGGGCCGAGGTCGGCCGCCATCGCTGGTGCTATTGGAGGGATTGCTGCTGCAGCTGCGGTGGCGGGCAAGCAGGCAGCGAAGAGATATGTGCCAATATAG

Coding sequence (CDS)

ATGGAAGATTCCCCGCAATCAGCCGGTGATCGGAAGACCCGTTTCTACCACCCCTACCAAGACCTTCACGTCCCGATCACCAAGCTTTACGAGCTTCCCACCGCCCCAGAACATCTTTTCTTCGAAGAGGCGGCTAGGCCCCACCGTTCTTGGGGCGAAAATCTCCAGTACTACACCGGAATCGGCTACTTGTCCGGTGCCCTTTTTGGCGGCGCCAGGGGATCTCTCCAGGGCATTAGGGCGGCGGAGCCTGGCGACACCATGAAGCTTCGTCTTAATCGAGTTTTGAACTCTGGGGGTCAGGTCGGCCGCAGGGCTGGGAACTCTCTTGGGATTCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTACTCATTTGAGAGGCTCTGATGATGTTTTGAATAGTATTGTTGCTGGATTGGGGACTGGTGCAATTTATAAGGCGGCTTCAGGGCCGAGGTCGGCCGCCATCGCTGGTGCTATTGGAGGGATTGCTGCTGCAGCTGCGGTGGCGGGCAAGCAGGCAGCGAAGAGATATGTGCCAATATAG

Protein sequence

MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI
Homology
BLAST of Cp4.1LG04g01900 vs. ExPASy Swiss-Prot
Match: Q9LNQ1 (Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis thaliana OX=3702 GN=TIM23-1 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-51
Identity = 106/182 (58.24%), Postives = 131/182 (71.98%), Query Frame = 0

Query: 4   SPQSAGDRKTRFYHPYQDLHVPITK--LYELPTAPEHLFFEEAARPHRSWGENLQYYTGI 63
           S     D  TR YHPYQ+  VPI    LY+LPT+PE LF EE+ +  RSWGENL +YTG 
Sbjct: 6   SSDHESDENTRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLTFYTGT 65

Query: 64  GYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLE 123
           GYL+G++ G + G   GI++ E GDT KL++NR+LNS GQ GR  GN +GI+GLI+AG+E
Sbjct: 66  GYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLIYAGIE 125

Query: 124 SGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYV 183
           SGV  +   DDV  S+VAGLGTGA+++AA G RSAA+AGA GGIAA A VAGKQ  KRY 
Sbjct: 126 SGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQVFKRYA 185

BLAST of Cp4.1LG04g01900 vs. ExPASy Swiss-Prot
Match: Q38820 (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis thaliana OX=3702 GN=TIM23-2 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-51
Identity = 104/177 (58.76%), Postives = 131/177 (74.01%), Query Frame = 0

Query: 10  DRKTRFYHPYQDLHVPITK---LYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSG 69
           D  TR Y+PYQ+  VPI K   LY+LPT+PE LF EEA R  RSWGENL +YTG  YL G
Sbjct: 12  DENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYTGTAYLGG 71

Query: 70  ALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTH 129
           ++ G + G + G+++ E GDT KL++NR+LNS GQ GR  GN +GI+GL++AG+ESG+  
Sbjct: 72  SVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAGIESGIVA 131

Query: 130 LRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
               DDV  S+VAGLGTGA+ +AA G RSAA+AGA+GG+AA A VAGKQ  KRYVPI
Sbjct: 132 ATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKRYVPI 188

BLAST of Cp4.1LG04g01900 vs. ExPASy Swiss-Prot
Match: Q9S837 (Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis thaliana OX=3702 GN=TIM23-3 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 3.8e-51
Identity = 101/184 (54.89%), Postives = 139/184 (75.54%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           M  S      +K R Y+PYQ +++P  KLYELPT+PE LF EEA +   +WGENL ++TG
Sbjct: 5   MNHSTGHQQQQKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTG 64

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
            GY +G++ G  +G++ G+RAAE G+++K+R NR+LNSGG V RR GN LG +GL+FA +
Sbjct: 65  WGYCTGSVLGAFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAM 124

Query: 121 ESGVTHLR-GSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKR 180
           ESGVT++R G D  L +++AGL TG +Y+AASGPRSA +AGA+GG+AA AAVAG++  KR
Sbjct: 125 ESGVTYMRDGDDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKR 184

Query: 181 YVPI 184
           +VPI
Sbjct: 185 FVPI 188

BLAST of Cp4.1LG04g01900 vs. ExPASy Swiss-Prot
Match: Q9USM7 (Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tim23 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 57/160 (35.62%), Postives = 84/160 (52.50%), Query Frame = 0

Query: 17  HPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFGGARGSL 76
           HP  DL  P+  L     A   L  +  A P R W ++L Y TG  YLSG   GG  G  
Sbjct: 45  HPLADLDKPLDYLLIEEDALSTLPGDSMAIPSRGWQDDLCYGTGTSYLSGLAIGGLWGLN 104

Query: 77  QGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRGSDDVLNS 136
           +G++  +   + +LRLN +LN   + G   GNSLG+L L++ G+ S + + R      NS
Sbjct: 105 EGMKKTKDITSTRLRLNGILNGVTRRGPFVGNSLGVLALVYNGINSLIGYKRQKHGWENS 164

Query: 137 IVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQA 177
           + AG  TGA+YK+  G R+ AI+ ++   AA      K++
Sbjct: 165 VAAGALTGALYKSTRGLRAMAISSSLVATAAGIWTLAKRS 204

BLAST of Cp4.1LG04g01900 vs. ExPASy Swiss-Prot
Match: P32897 (Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIM23 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.5e-12
Identity = 50/125 (40.00%), Postives = 69/125 (55.20%), Query Frame = 0

Query: 47  PHRSWGENLQYYTGIGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRA 106
           P R W ++L Y TG  YL G   GG  G +QG++   P    KL+LN VLN   + G   
Sbjct: 89  PSRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQLNTVLNHITKRGPFL 148

Query: 107 GNSLGILGLIFAGLESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIA 166
           GN+ GIL L +  + S +  LRG  D   SI AG  TGA++K++ G +    + A+  +A
Sbjct: 149 GNNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGALFKSSKGLKPMGYSSAM--VA 208

Query: 167 AAAAV 172
           AA AV
Sbjct: 209 AACAV 211

BLAST of Cp4.1LG04g01900 vs. NCBI nr
Match: XP_023531226.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352 bits (904), Expect = 2.86e-122
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY
Sbjct: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. NCBI nr
Match: XP_022925513.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita moschata] >KAG7020879.1 Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349 bits (896), Expect = 4.74e-121
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGA+FGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGAIFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. NCBI nr
Match: XP_023002094.1 (mitochondrial import inner membrane translocase subunit TIM23-3-like [Cucurbita maxima])

HSP 1 Score: 349 bits (895), Expect = 6.74e-121
Identity = 182/183 (99.45%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MEDSPQSAG RKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSPQSAGYRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY
Sbjct: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. NCBI nr
Match: KAG6588317.1 (Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 348 bits (892), Expect = 1.93e-120
Identity = 180/183 (98.36%), Postives = 181/183 (98.91%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGA+FGGARGSLQGIR AEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGAIFGGARGSLQGIRTAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. NCBI nr
Match: XP_008462719.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-2 [Cucumis melo] >KAA0062574.1 mitochondrial import inner membrane translocase subunit TIM23-2 [Cucumis melo var. makuwa] >TYK28799.1 mitochondrial import inner membrane translocase subunit TIM23-2 [Cucumis melo var. makuwa])

HSP 1 Score: 329 bits (843), Expect = 5.71e-113
Identity = 169/183 (92.35%), Postives = 177/183 (96.72%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           ME+S +S  DRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGS+QG+RAAEPGD++KLRLNRVLNSGGQ+GRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQA KRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. ExPASy TrEMBL
Match: A0A6J1EFE6 (mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbita moschata OX=3662 GN=LOC111432787 PE=4 SV=1)

HSP 1 Score: 349 bits (896), Expect = 2.30e-121
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGA+FGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGAIFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. ExPASy TrEMBL
Match: A0A6J1KPG9 (mitochondrial import inner membrane translocase subunit TIM23-3-like OS=Cucurbita maxima OX=3661 GN=LOC111496064 PE=4 SV=1)

HSP 1 Score: 349 bits (895), Expect = 3.26e-121
Identity = 182/183 (99.45%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MEDSPQSAG RKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSPQSAGYRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY
Sbjct: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. ExPASy TrEMBL
Match: A0A0A0M0I6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690355 PE=4 SV=1)

HSP 1 Score: 330 bits (845), Expect = 1.37e-113
Identity = 169/183 (92.35%), Postives = 178/183 (97.27%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           ME+S +S  DRK+RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGALFGGARGS+QG+RAAEPGD++KLRLNRVLNSGGQ+GRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQA KRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. ExPASy TrEMBL
Match: A0A5A7V5P8 (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G001650 PE=4 SV=1)

HSP 1 Score: 329 bits (843), Expect = 2.76e-113
Identity = 169/183 (92.35%), Postives = 177/183 (96.72%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           ME+S +S  DRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGS+QG+RAAEPGD++KLRLNRVLNSGGQ+GRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQA KRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. ExPASy TrEMBL
Match: A0A1S3CJ69 (mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo OX=3656 GN=LOC103501018 PE=4 SV=1)

HSP 1 Score: 329 bits (843), Expect = 2.76e-113
Identity = 169/183 (92.35%), Postives = 177/183 (96.72%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           ME+S +S  DRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGS+QG+RAAEPGD++KLRLNRVLNSGGQ+GRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRY 180
           ESGV HLRGSDDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQA KRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 183
           VPI
Sbjct: 181 VPI 183

BLAST of Cp4.1LG04g01900 vs. TAIR 10
Match: AT1G17530.1 (translocase of inner mitochondrial membrane 23 )

HSP 1 Score: 203.8 bits (517), Expect = 1.2e-52
Identity = 106/182 (58.24%), Postives = 131/182 (71.98%), Query Frame = 0

Query: 4   SPQSAGDRKTRFYHPYQDLHVPITK--LYELPTAPEHLFFEEAARPHRSWGENLQYYTGI 63
           S     D  TR YHPYQ+  VPI    LY+LPT+PE LF EE+ +  RSWGENL +YTG 
Sbjct: 6   SSDHESDENTRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLTFYTGT 65

Query: 64  GYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLE 123
           GYL+G++ G + G   GI++ E GDT KL++NR+LNS GQ GR  GN +GI+GLI+AG+E
Sbjct: 66  GYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLIYAGIE 125

Query: 124 SGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYV 183
           SGV  +   DDV  S+VAGLGTGA+++AA G RSAA+AGA GGIAA A VAGKQ  KRY 
Sbjct: 126 SGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQVFKRYA 185

BLAST of Cp4.1LG04g01900 vs. TAIR 10
Match: AT1G72750.1 (translocase inner membrane subunit 23-2 )

HSP 1 Score: 203.8 bits (517), Expect = 1.2e-52
Identity = 104/177 (58.76%), Postives = 131/177 (74.01%), Query Frame = 0

Query: 10  DRKTRFYHPYQDLHVPITK---LYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSG 69
           D  TR Y+PYQ+  VPI K   LY+LPT+PE LF EEA R  RSWGENL +YTG  YL G
Sbjct: 12  DENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYTGTAYLGG 71

Query: 70  ALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTH 129
           ++ G + G + G+++ E GDT KL++NR+LNS GQ GR  GN +GI+GL++AG+ESG+  
Sbjct: 72  SVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAGIESGIVA 131

Query: 130 LRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
               DDV  S+VAGLGTGA+ +AA G RSAA+AGA+GG+AA A VAGKQ  KRYVPI
Sbjct: 132 ATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKRYVPI 188

BLAST of Cp4.1LG04g01900 vs. TAIR 10
Match: AT3G04800.1 (translocase inner membrane subunit 23-3 )

HSP 1 Score: 202.6 bits (514), Expect = 2.7e-52
Identity = 101/184 (54.89%), Postives = 139/184 (75.54%), Query Frame = 0

Query: 1   MEDSPQSAGDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           M  S      +K R Y+PYQ +++P  KLYELPT+PE LF EEA +   +WGENL ++TG
Sbjct: 5   MNHSTGHQQQQKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTG 64

Query: 61  IGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGL 120
            GY +G++ G  +G++ G+RAAE G+++K+R NR+LNSGG V RR GN LG +GL+FA +
Sbjct: 65  WGYCTGSVLGAFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAM 124

Query: 121 ESGVTHLR-GSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKR 180
           ESGVT++R G D  L +++AGL TG +Y+AASGPRSA +AGA+GG+AA AAVAG++  KR
Sbjct: 125 ESGVTYMRDGDDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKR 184

Query: 181 YVPI 184
           +VPI
Sbjct: 185 FVPI 188

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LNQ11.7e-5158.24Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis t... [more]
Q388201.7e-5158.76Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis t... [more]
Q9S8373.8e-5154.89Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis t... [more]
Q9USM72.0e-1535.63Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharom... [more]
P328973.5e-1240.00Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces c... [more]
Match NameE-valueIdentityDescription
XP_023531226.12.86e-122100.00mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita ... [more]
XP_022925513.14.74e-12198.91mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita ... [more]
XP_023002094.16.74e-12199.45mitochondrial import inner membrane translocase subunit TIM23-3-like [Cucurbita ... [more]
KAG6588317.11.93e-12098.36Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurb... [more]
XP_008462719.15.71e-11392.35PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-2 [Cucu... [more]
Match NameE-valueIdentityDescription
A0A6J1EFE62.30e-12198.91mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbit... [more]
A0A6J1KPG93.26e-12199.45mitochondrial import inner membrane translocase subunit TIM23-3-like OS=Cucurbit... [more]
A0A0A0M0I61.37e-11392.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690355 PE=4 SV=1[more]
A0A5A7V5P82.76e-11392.35Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo ... [more]
A0A1S3CJ692.76e-11392.35mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo ... [more]
Match NameE-valueIdentityDescription
AT1G17530.11.2e-5258.24translocase of inner mitochondrial membrane 23 [more]
AT1G72750.11.2e-5258.76translocase inner membrane subunit 23-2 [more]
AT3G04800.12.7e-5254.89translocase inner membrane subunit 23-3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF02466Tim17coord: 57..165
e-value: 1.2E-23
score: 83.7
NoneNo IPR availablePANTHERPTHR15371:SF24MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM23-3coord: 1..183
NoneNo IPR availablePANTHERPTHR15371TIM23coord: 1..183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g01900.1Cp4.1LG04g01900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:0005744 TIM23 mitochondrial import inner membrane translocase complex
molecular_function GO:0008320 protein transmembrane transporter activity