Cp4.1LG04g00620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g00620
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Isoaspartyl peptidase/L-asparaginase
Location	Cp4.1LG04: 1432113 .. 1434898 (+)
RNA-Seq Expression	Cp4.1LG04g00620
Synteny	Cp4.1LG04g00620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTTACATCGAACAAAGCAAGCCTTCCAATAATCTCACGTCATGTCATCAAGACGACTAAGCGGCTCTCTCTGCCTTCATCCTCGACAGATTGGAGCAATTGCATGAACCCTCCCGTAAATCGTCGGCGATCGATCTTCTAAATTACCTAGGGTTGGTGGTTTGATCCATTGGCGCGGGACCTAAATGGGTTGGGCAATTGCTCTTCACGGCGGCGCCGGCGACATCCCACTGTCTTTGTCATTGGACCGTCGCCAACCCCGCGAGGAAACCCTCCGTCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAAGAGCAGAAACCTCCAGTGGATGTTGTTGAACTTGTGGTGAATATTATTTACTCCCTCTCTCTGTTTTTCTACCACTCTGGTTTAATATAAAATTGTTTCGATTAAGTTTGTGTAATCTTAGAGTTCGTAACGATTTTGTCTATTGATTTGTGTGCTTAACGATTTCTTTCACAATGTTGTTTAAAATTTGTAATGATTGATCTGTAATGTAAAAAATTCCAATAGGTTGTAATGGAATTTCTGTGAGTGTATAGATGGATGAATTGCTTGGAGAACAAATGTGTATTTCATTTTTGTAAGAAACCAAAATGAAATTCTCAATGACATACAAAAAATAAACATCGTGATTACAAGTACTGTAGTACAGATTATCTCGACTTGACGATCAATTCATGGTTATATATGGCTTCTTTTCCATGGTAAGATGGTCAGCTTTTGAACTGTAGGTTCTCTTACACTTTCCCGTGCTGTTGAATTTGTTCTTGTACAGGTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACCGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGTTAGAATTTCTACCTCGTTCCTATGTACACAGATAGATGCTTTGCGATTCATGACTGTTTTATTTGTGCACCAATTTCTTATCTGTTCAATTTTCTTAAAATTGTCAAGGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCCAACAGTGTGCAGGTTTGCTTCTTATCTTTGTTGGGAGATAGTCCCACATTAGCTAATTTAGTGAATGATCATGGGTTTATAAGTAAGGAATACATCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAGCCATTAGAGCTTATACTCAAACTGGACAACATCATACCATTGTGGTGAGTCGTTATTTCTAACATGGTATCAGAGTCATACCCTTTATTTAGTCATGTCAATAGAATCGTCAAATGTCGAACAAAGAAGTTGTGAGCCTCGAAGGTGTAGTCAAAAGTGACTCATGTGTCGAACAAAGGGTGTACTTTGTTTGAGGGCTCCCGAGAAAAGGAGTCAACCTCGATTAAGGGGAGGATATTCGAGGGCTATATAGAACTCAGGGGCTCTATGGTGTACTTTGTTCGATAGGAGGATTGTTGAAGATTGTTGGGAGAGAATTCCACATTGGCTAATTTAGGGAATGATCATTGGTTTATAAGTAAGGAATACATCTACCAGGGAAGCCCAAAGCAAAGCCATTAGAGCTTATACTCAAAGTGGACAATATCATACCGTGGTGGAGAGTCGTTATTCCTAACAATCTTCTATCCCAAACATGACTTTGACTGCTCTCTTTATGAACCAAAAGTTTATAGGTCTAGATATCCGTCTAATAGGGTTGAAAAAATCACATTATGGAGTTTCCATGAGATTTTTATGTTTTAGCATCGGTTGATTTCTATGGTACTTTTTGTATCTGCACATTGATAGCTGTTGTCATAAACACTTATAGATTGATTATACTCAACCAAGTCCCCAAGCCACAGGACAAGAAACTGAGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGATACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAATTCAAGGGTCTATCTCTTGAAGAAGCAGCAGCCTATGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCGGCCTCGGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCCAACATGACTGAATGAATGTTGTTGCCGCGAAGCCTGTACTGAAGTTTGGTTCTATCTTCAGCTGTCAGAGAAATCCTATGTTGTTTCCTTCCTAAAGTATTAAGAATAGCCTTCCAAAGGATCTTTCGTTTTCATTTATTTGGTTGGATTTCAATGGCTAGCTTTTCTTTAAACACCGTTAACAATATATATATATATATATATATATATATATATATGTTTTTTATTTTTGACCTAACTTTCAAAACCCAAGGCCAACTCATGTTCGTTTTCATGTCTGTTAAATGTCATTTTAAAGTAGAAGAACAAAAAC

mRNA sequence

TGGTTACATCGAACAAAGCAAGCCTTCCAATAATCTCACGTCATGTCATCAAGACGACTAAGCGGCTCTCTCTGCCTTCATCCTCGACAGATTGGAGCAATTGCATGAACCCTCCCGTAAATCGTCGGCGATCGATCTTCTAAATTACCTAGGGTTGGTGGTTTGATCCATTGGCGCGGGACCTAAATGGGTTGGGCAATTGCTCTTCACGGCGGCGCCGGCGACATCCCACTGTCTTTGTCATTGGACCGTCGCCAACCCCGCGAGGAAACCCTCCGTCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAAGAGCAGAAACCTCCAGTGGATGTTGTTGAACTTGTGGTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACCGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAATTGATTATACTCAACCAAGTCCCCAAGCCACAGGACAAGAAACTGAGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGATACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAATTCAAGGGTCTATCTCTTGAAGAAGCAGCAGCCTATGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCGGCCTCGGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCCAACATGACTGAATGAATGTTGTTGCCGCGAAGCCTGTACTGAAGTTTGGTTCTATCTTCAGCTGTCAGAGAAATCCTATGTTGTTTCCTTCCTAAAGTATTAAGAATAGCCTTCCAAAGGATCTTTCGTTTTCATTTATTTGGTTGGATTTCAATGGCTAGCTTTTCTTTAAACACCGTTAACAATATATATATATATATATATATATATATATATATGTTTTTTATTTTTGACCTAACTTTCAAAACCCAAGGCCAACTCATGTTCGTTTTCATGTCTGTTAAATGTCATTTTAAAGTAGAAGAACAAAAAC

Coding sequence (CDS)

ATGGGTTGGGCAATTGCTCTTCACGGCGGCGCCGGCGACATCCCACTGTCTTTGTCATTGGACCGTCGCCAACCCCGCGAGGAAACCCTCCGTCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAAGAGCAGAAACCTCCAGTGGATGTTGTTGAACTTGTGGTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACCGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAATTGATTATACTCAACCAAGTCCCCAAGCCACAGGACAAGAAACTGAGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGATACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAATTCAAGGGTCTATCTCTTGAAGAAGCAGCAGCCTATGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCGGCCTCGGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCCAACATGACTGAATGA

Protein sequence

MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFAREQIDYTQPSPQATGQETEIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIWPSNMTE

Homology

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BLAST of Cp4.1LG04g00620 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.1e-119
Identity = 231/322 (71.74%), Postives = 253/322 (78.57%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENC 61
           GW+IALHGGAGDIP SL  +RRQPREE LR CLQIGV+ALK QKPP+DVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
            HFNAG GSVLT +GTVEMEASIMDGK  +CGAVSGL+TV+N ISLARLVM+KTPHIYLA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFARE----------------------------------QIDYTQPSPQATGQET 181
           F GA+ FA++                                  Q +Y QP+     +E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKEL 182

Query: 182 EIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSA 241
            + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFN 288
           TGKGEAII  TVARDVAALMEFKGLSL+EAA Y V E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFN 302

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 428.3 bits (1100), Expect = 6.8e-119
Identity = 230/319 (72.10%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ L  +RR PRE  LR CL +G+ ALK  KPP+DV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYL 120
            P FNAG+GSVLT  GTVEMEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFARE-----------------------------QIDYTQPSPQATGQETEIGN 180
           AFD AEAFAR                              Q+DYT PSP+          
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPD-----NC 181

Query: 181 GDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKG 240
           GDSQIGTVGCVAVD+ GNLA+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 289
           E IIRGTVARDVAALME+KGLSL EAAAY V +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.0e-118
Identity = 231/322 (71.74%), Postives = 255/322 (79.19%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENC 61
           GW+IALHGGAGDIP SL  +RR+PREE LR CLQIGV+ALK QKPP+DVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
            HFNAG GSVLT +GTVEMEASIMDG   +CGAVSGL+TV+N ISLARLVM+KTPHIYLA
Sbjct: 63  EHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFARE-----------------------------QIDYTQ---PSPQATGQETE- 181
           F GA+ FA++                             Q+DY+Q   P P     E E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKEL 182

Query: 182 -IGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSA 241
            + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSA
Sbjct: 183 PLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFN 288
           TGKGE IIR TVARDVAALMEFKGLSL+EAA + + E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAGEIAMPFN 302

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.1e-116
Identity = 225/322 (69.88%), Postives = 248/322 (77.02%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENC 61
           GW+IALHGGAGDIP SL  +RR+PREE LR CLQIG++ALK Q PP+DVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
            HFNAG GSVLT +GTVEMEASIMDG   +CGAVSGL TV+N ISLAR VM+KTPHI+LA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLA 122

Query: 122 FDGAEAFARE----------------------------------QIDYTQPSPQATGQET 181
           F GA+   ++                                  Q +YTQP      +E 
Sbjct: 123 FQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKEL 182

Query: 182 EIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSA 241
            + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFN 288
           TGKGEAIIR TVARDVAALMEFKGLSL+EAA   V E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAGEIAMPFN 302

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy Swiss-Prot
Match: P30362 (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.7e-107
Identity = 213/302 (70.53%), Postives = 235/302 (77.81%), Query Frame = 0


Query: 21  DRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENCPHFNAGRGSVLTTNGTVEM 80
           +RR+PREE LR CLQIGV+ALK +K P+DVVELVVRELEN  HFNAG GSVLT +GTVEM
Sbjct: 3   ERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTVEM 62

Query: 81  EASIMDGKK-RCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFARE--------- 140
           EASIMDGK  +CGAVSGL+TV+N ISLARLVMEKTPH+YLAF GA+ FA++         
Sbjct: 63  EASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVDSS 122

Query: 141 -------------------------QIDYTQPSPQATGQETEIGNGDSQIGTVGCVAVDN 200
                                    Q +YTQP      +E  + NGDSQIGTVGCVAVD+
Sbjct: 123 HFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAVDS 182

Query: 201 HGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAAL 260
            GNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSATGKGEAII+ TVARDVAAL
Sbjct: 183 QGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVAAL 242

Query: 261 MEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAI 287
           MEFKGLSL+EAA Y V E  PKG VGLIAVSA+GE+AMPFNTTGMFRACATEDG SEIAI
Sbjct: 243 MEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGNSEIAI 302

[Close]

BLAST of Cp4.1LG04g00620 vs. NCBI nr
Match: XP_023530268.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555 bits (1430), Expect = 2.15e-198
Identity = 291/320 (90.94%), Postives = 291/320 (90.94%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAEAFAREQ                             IDYTQPSPQATGQETEIGNG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATGQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 291
           AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. NCBI nr
Match: KAG6588471.1 (Isoaspartyl peptidase/L-asparaginase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 546 bits (1407), Expect = 6.88e-195
Identity = 287/320 (89.69%), Postives = 289/320 (90.31%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAEAFAREQ                             IDYT+PSPQAT QET IGNG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPHNIERLKQAKEANSVQIDYTRPSPQATVQETGIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 291
           AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. NCBI nr
Match: XP_022933679.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita moschata])

HSP 1 Score: 543 bits (1399), Expect = 1.14e-193
Identity = 286/320 (89.38%), Postives = 288/320 (90.00%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAEAFAREQ                             IDYTQPSPQAT QETEIGNG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 291
           AIIRGTVARDVAALMEFK LSLEEAAA AVESVPKGNVGLIAVSASG+VAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. NCBI nr
Match: XP_022969815.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita maxima])

HSP 1 Score: 537 bits (1384), Expect = 2.20e-191
Identity = 284/320 (88.75%), Postives = 285/320 (89.06%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCL IGVQALKEQKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAEAFAREQ                             IDYTQPSPQAT QET IGNG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETGIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 291
           AIIRGTVARDVAALMEFK LSLE AAA AVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEAAAACAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. NCBI nr
Match: XP_038878292.1 (isoaspartyl peptidase/L-asparaginase isoform X2 [Benincasa hispida])

HSP 1 Score: 510 bits (1313), Expect = 8.34e-181
Identity = 264/302 (87.42%), Postives = 272/302 (90.07%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIP SL  +RRQPREETLRRCLQIGVQALK QKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GT+EMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ---------------IDYTQPSPQATGQETEIGNGDSQIGTVGCVAVDN 180
           FDGAEAFAREQ               IDYTQP PQAT QETE  NGDSQIGTVGCVAVDN
Sbjct: 121 FDGAEAFAREQNIERLKQAKEANRVQIDYTQPLPQATEQETENANGDSQIGTVGCVAVDN 180

Query: 181 HGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAAL 240
           HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA+SATGKGEAIIRGTVARDVAAL
Sbjct: 181 HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGEAIIRGTVARDVAAL 240

Query: 241 MEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIW 287
           MEFKGLSLEEA AY VESVPKGNVGL+AVSASGEVA+P+NTTGMFRACATEDGYSEIAIW
Sbjct: 241 MEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFRACATEDGYSEIAIW 300

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy TrEMBL
Match: A0A6J1F0F1 (isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC111441019 PE=4 SV=1)

HSP 1 Score: 543 bits (1399), Expect = 5.51e-194
Identity = 286/320 (89.38%), Postives = 288/320 (90.00%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAEAFAREQ                             IDYTQPSPQAT QETEIGNG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 291
           AIIRGTVARDVAALMEFK LSLEEAAA AVESVPKGNVGLIAVSASG+VAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy TrEMBL
Match: A0A6J1I105 (isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita maxima OX=3661 GN=LOC111468901 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 1.06e-191
Identity = 284/320 (88.75%), Postives = 285/320 (89.06%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCL IGVQALKEQKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAEAFAREQ                             IDYTQPSPQAT QET IGNG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETGIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 291
           AIIRGTVARDVAALMEFK LSLE AAA AVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEAAAACAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy TrEMBL
Match: A0A5A7UBC6 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00060 PE=4 SV=1)

HSP 1 Score: 504 bits (1299), Expect = 9.12e-179
Identity = 266/316 (84.18%), Postives = 271/316 (85.76%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSL  DRRQPREETLRRCLQIGVQALK QKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAE FAREQ                             IDYTQP PQAT QETE  NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA+SATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 287
           AIIR TVARDVAALMEFKGLSLEEAAA  VESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy TrEMBL
Match: A0A5D3CEE4 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003800 PE=4 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 5.27e-178
Identity = 265/316 (83.86%), Postives = 270/316 (85.44%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSL  DRRQPREETLRRCLQIGVQALK QKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAE FAREQ                             IDYTQP PQAT QETE  NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DS IGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA+SATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 287
           AIIR TVARDVAALMEFKGLSLEEAAA  VESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. ExPASy TrEMBL
Match: A0A1S3BPR4 (isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=4 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 5.27e-178
Identity = 265/316 (83.86%), Postives = 270/316 (85.44%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSL  DRRQPREETLRRCLQIGVQALK QKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GTVEMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQ-----------------------------IDYTQPSPQATGQETEIGNG 180
           FDGAE FAREQ                             IDYTQP PQAT QETE  NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240
           DS IGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA+SATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 287
           AIIR TVARDVAALMEFKGLSLEEAAA  VESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

[Close]

BLAST of Cp4.1LG04g00620 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 428.3 bits (1100), Expect = 4.8e-120
Identity = 230/319 (72.10%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ L  +RR PRE  LR CL +G+ ALK  KPP+DV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYL 120
            P FNAG+GSVLT  GTVEMEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFARE-----------------------------QIDYTQPSPQATGQETEIGN 180
           AFD AEAFAR                              Q+DYT PSP+          
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPD-----NC 181

Query: 181 GDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKG 240
           GDSQIGTVGCVAVD+ GNLA+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 289
           E IIRGTVARDVAALME+KGLSL EAAAY V +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

[Close]

BLAST of Cp4.1LG04g00620 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.3e-85
Identity = 174/240 (72.50%), Postives = 188/240 (78.33%), Query Frame = 0


Query: 80  MEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFARE-------- 139
           MEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYLAFD AEAFAR         
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 ---------------------QIDYTQPSPQATGQETEIGNGDSQIGTVGCVAVDNHGNL 199
                                Q+DYT PSP+          GDSQIGTVGCVAVD+ GNL
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPKVPD-----NCGDSQIGTVGCVAVDSAGNL 120

Query: 200 AAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAALMEFK 259
           A+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKGE IIRGTVARDVAALME+K
Sbjct: 121 ASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVAALMEYK 180

Query: 260 GLSLEEAAAYAV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIWPSN 289
           GLSL EAAAY V +SVP+G+ GL+AVSA+GEV MPFNTTGMFRACA+EDGYSEIAIWP+N
Sbjct: 181 GLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEIAIWPNN 235

[Close]

BLAST of Cp4.1LG04g00620 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.3e-84
Identity = 176/323 (54.49%), Postives = 215/323 (66.56%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENC 61
           GWAIA+HGGAG  P +L  +R++  ++ L RCL +G+ AL+     +DVVELV+RELE  
Sbjct: 3   GWAIAVHGGAGIDP-NLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETD 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDG-KKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
           P FN+GRGS LT  GTVEMEASIMDG K+RCGAVSG+TTV N ISLARLVM+K+PH YLA
Sbjct: 63  PLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLA 122

Query: 122 FDGAEAFAREQ-----------------------------IDYTQP-----SPQATGQET 181
           F GAE FAR+Q                              DY  P        AT    
Sbjct: 123 FSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPI 182

Query: 182 EIGNGDSQI---GTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 241
           ++      I    TVGCV VD  G+ AA TSTGGL+NKM+GRIGD+PLIGAGTYA+  C 
Sbjct: 183 QMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 242

Query: 242 VSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVE-SVPKGNVGLIAVSASGEVAM 286
           VS TG+GEAIIR T+ARDV+A+ME+KGL+L+EA  Y ++  + +G  GLIAVS  GEV  
Sbjct: 243 VSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVVC 302

[Close]

BLAST of Cp4.1LG04g00620 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 3.2e-15
Identity = 83/335 (24.78%), Postives = 134/335 (40.00%), Query Frame = 0


Query: 5   IALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENCPHF 64
           +A+H GAG      + + +  R    R CL       ++    +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTNGTVEMEASIMDGKK-RCGAVSGLTTVVNAISLARLVME----------K 124
           NAGRGS LT +G VE +AS+MDG     G V  +  V NAI +A L+++          +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 125 TPHIYLAFDGAEAFARE----------QIDYTQPSPQATGQ-------ETEIG------- 184
            P + L  +GA  + +           + D    + +A  Q        +E+G       
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 185 -----------------------NGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRI 244
                                  + D  + TVG + VDN G++A  +S+GG+  K+ GR+
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRV 254

Query: 245 GDTPLIGAGTYAN--------HLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAA 264
           G     G+G +A+         L     +G GE ++RG  AR+    +         AA 
Sbjct: 255 GLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAM 314

[Close]

BLAST of Cp4.1LG04g00620 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 3.0e-13
Identity = 83/322 (25.78%), Postives = 131/322 (40.68%), Query Frame = 0


Query: 5   IALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELENCPHF 64
           +A+H GAG      + + +  R    R CL       ++    +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAIS--LARLVM----------- 124
           NAGRGS LT +G VE +AS+MDG    G   G+  V + +S  L++L++           
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS--GMFGGVGAVPDQVSEMLSKLLLCWLLVGEGARR 134

Query: 125 -EKTPHIYLAFDGAEA-------FAREQ---------------IDYTQPSPQAT-GQET- 184
             K+  + +     EA        AR Q               I   +  P+ T   ET 
Sbjct: 135 WGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTENNETC 194

Query: 185 -------EIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYAN 244
                     + D  + TVG + VDN G++A  +S+GG+  K+ GR+G     G+G +A+
Sbjct: 195 EENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWAS 254

Query: 245 --------HLCAVSATGKGEAIIRGTVARDVAALMEFKGLSLEEAAAYAVESV------- 264
                    L     +G GE ++RG  AR+    +         AA   + SV       
Sbjct: 255 SKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSK 314

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g00620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30364	3.1e-119	71.74	Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1	[more]
P50287	6.8e-119	72.10	Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08...	[more]
Q9ZSD6	2.0e-118	71.74	Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1	[more]
P50288	4.1e-116	69.88	Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1	[more]
P30362	2.7e-107	70.53	Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2...	[more]

BLAST of Cp4.1LG04g00620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530268.1	2.15e-198	90.94	isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6588471.1	6.88e-195	89.69	Isoaspartyl peptidase/L-asparaginase 1, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022933679.1	1.14e-193	89.38	isoaspartyl peptidase/L-asparaginase 1 [Cucurbita moschata]	[more]
XP_022969815.1	2.20e-191	88.75	isoaspartyl peptidase/L-asparaginase 1 [Cucurbita maxima]	[more]
XP_038878292.1	8.34e-181	87.42	isoaspartyl peptidase/L-asparaginase isoform X2 [Benincasa hispida]	[more]

BLAST of Cp4.1LG04g00620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0F1	5.51e-194	89.38	isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1I105	1.06e-191	88.75	isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114689...	[more]
A0A5A7UBC6	9.12e-179	84.18	Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5D3CEE4	5.27e-178	83.86	Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BPR4	5.27e-178	83.86	isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=...	[more]

BLAST of Cp4.1LG04g00620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08100.1	4.8e-120	72.10	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	1.3e-85	72.50	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT3G16150.1	4.3e-84	54.49	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.1	3.2e-15	24.78	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	3.0e-13	25.78	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 157..288 e-value: 4.0E-45 score: 155.2
None	No IPR available	PANTHER	PTHR10188:SF33	ISOASPARTYL PEPTIDASE/L-ASPARAGINASE	coord: 131..285 coord: 1..131
None	No IPR available	CDD	cd04701	Asparaginase_2	coord: 4..278 e-value: 2.69771E-131 score: 371.409
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 3..285 e-value: 1.3E-97 score: 326.6
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 131..285 coord: 1..131
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 3..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g00620.1	Cp4.1LG04g00620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016540	protein autoprocessing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004067	asparaginase activity
molecular_function	GO:0008798	beta-aspartyl-peptidase activity
molecular_function	GO:0016787	hydrolase activity